CN1295785A - Method for treating seed in ac electric field - Google Patents
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- CN1295785A CN1295785A CN 00135603 CN00135603A CN1295785A CN 1295785 A CN1295785 A CN 1295785A CN 00135603 CN00135603 CN 00135603 CN 00135603 A CN00135603 A CN 00135603A CN 1295785 A CN1295785 A CN 1295785A
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Abstract
The seed-pretreating method combining D.C. Field (electrostatic field) with conventional seed treatment process includes the following steps: soaking seed in a nutrient solution consisting of ABT rooting powder, GA gibberellin, PEG regulator and any other chemical elements, using D.C field to treat seed, then dressing seed with ash powder Ca(OH)2. Said invented method possesses the double effects of D.C. field and chemical, hormone and trace element treatment so as to improve physical and chemical indexes of biological electric field, electric conductivity, enzyme activity and ionic activity of tree seed, and at the same time, can greatly raise vitality of seed. For Chinese pine and masson pine, the seedling rate can be raised by 15-57% and the plant growth rate can be raised by 10-30%. It also is applicable to pretreatment of other plant seeds.
Description
The present invention relates to a kind of scattered seeds by airplane for afforestation, the middle seed processing technology of growing seedlings, particularly utilize DC electric field and chemistry, hormone, trace element and medicine etc. seed to be carried out the method for comprehensive pretreatment.
As everyone knows, in any type of plantation is produced, all relate to the process that seed is converted into seedling, especially aerial seeding, breeding and afforestation and agricultural kind, herbage kind, medicinal material kind, vegetables kind etc. all will be carried out strict seed treatment prior to seeding.Because all seeds reduce from collecting the moisture that exists between sowing time, each organ contraction, breathing and enzymic activity reduce or plant skin depth, and saturating property is poor, reasons such as germination inhibitor, thereby just caused the germination period of seed to reach tens days even situation for more time.The machine that this situation has just caused harm for mouse, bird, insect pest etc., a tree name are added up its loss and hazard ratio up to 50-70%.Particularly under field conditions (factors), after the seeds of plant seeds by airplane land, become seedling and need experience for a long time to taking root by absorbing water, expand, sprout, germinateing, this is very unfavorable to one-tenth seedling of building extensive forest and other plant husbandry.Thereby prior to seeding, particularly before the machine sowing seeds seeds are handled the important means that becomes promotion seed germination and growth.As sowing Chinese pine (Pinus tabulaeformis Carr.), masson pine seeds such as (P.massoniana Lamb.), all at first handle seed.The method of preliminary treatment seed roughly has four kinds both at home and abroad at present: the one, soak seed dressing with chemistry, medicine; The 2nd, with hormone such as ABT, GA, PEG and trace element seed dressing etc.; The 3rd, with the water logging kind or the employing Pelleting coating technology of different temperatures; The 4th, handle with physical agent list factor, as illumination penetrate, magnetic field method, low, high frequency, microwave treatment and DC electric field (electrostatic field) handle.Though these processing methods all have certain effect to changing aspects such as seed physiological status, promotion seed germination, raising Cheng Miao.But, but exist processing method single, process is loaded down with trivial details, the time is long, and the produce effects phase is short; Handled uniformity, the poor repeatability of seed, it is slow to improve the sprouting characteristic; Each seed of handling needs to buy medicine; People, animal etc. there is side effect and handles the high deficiency of seed costs.For example, patent of invention CN87105063.3 discloses a kind of electrostatic field methods for the treatment of seed of using, it is that the seed individual layer is sprinkling upon on the grounding plate, and certain altitude sets up many lamettas above metallic plate, is applied to high-tension electricity between metal wire and metallic plate and forms the electric field of certain field intensity.Seed improves activation capacity through electric field treatment, promotes to sprout and growth of seedling.But this processing method needs to add higher voltage because of being placed on seed on the metallic plate away from electrode, makes troubles to safety operation; Because the field intensity that forms because of energising between metal wire and the metallic plate is inhomogeneous, be difficult to guarantee that every seed obtains handling, thereby the uniformity and the repeatability of seed are handled in influence simultaneously; Because individual layer is placed on seed on the metallic plate, be difficult to guarantee to handle the quality of seed in addition.Patent report and product that Electrostatic Treatment seed device aspect is also arranged in recent years.As the described variable electric field seed processing machine of 96215980 patents, described electrostatic field of 92218112 patents and compound field seed processing machine; Described hf and hv pulse seed treatment instrument of 91201363 patents or the like.The equal processing method of these patented products is single and be difficult to improve significantly the sprouting index.Disclosed prepreparing tree-seeds for aerosowing preprocess method of patent of invention ZL93121206.5 and device ", also be because the single factor of employing DC electric field is handled, thereby still have seed germination amplitude and the low defective of physical and chemical index.
The objective of the invention is to overcome the deficiencies in the prior art, empirical theory and conventional method according to seed electrobiophysics effect, the integrated treatment kind submethod that a kind of DC electric field combines with routine techniques is proposed, with performance electric field, chemistry, hormone-treated double action and effect, improve seed germination index amplitude.
The technical scheme that realizes the object of the invention is, at first adopts certain density ABT root-inducing powder, GA gibberellin, PEG conditioning agent or composite fertilizer's solution seed soaking, or the seed dressing certain hour, applies electric field to seed then and handles.Its concrete steps are:
1. the nutritive solution of forming with ABT root-inducing powder, GA gibberellin, PEG conditioning agent, medicament and other any chemical element of 25-100ppm, trace element etc., is 1 in seed with the solution weight ratio: 0.1-3 ratio doubly, seed stirred soaked 3-24 hour, carry out preliminary treatment;
2. pretreated seed is put into plate electrode, applying electric-field intensity is 100-500kV/m, DC electric field, handled 10-30 minute, to change the saturating property of bio-electric field, ion concentration distribution, kind skin of seed, electrical conductivity is breathed and enzymic activity;
3. in the seed that electric field treatment is crossed, admix 1-5%Ca (OH)
2Powder is to increase immunising seed power.
The above-mentioned DC electric field of the present invention is handled, and is divided into positive electric field seed treatment and negative electric field seed treatment, as shown in the figure.
Fig. 1 is a positive electric field seed treatment schematic diagram
Fig. 2 is a negative electric field seed treatment schematic diagram
In Fig. 1, Fig. 2, the 1st, the positive electricity pole plate; The 2nd, negative electrode plate; The 3rd, the high direct voltage power supply, its output is linear adjustable, and amplitude is 5-20kV; 4 and 5 is high-tension bus-bar; 6 is earth connection, and 2 and 1 forms parallel-plate processes electrode.
Choose any one kind of them first before the electric field treatment and be diluted in the water such as ABT, PEG, GA or other nutriment, its concentration is 25-100ppm, then the ratio of seed and solution weight by 1: 0.1-3 3-24 hour (the deciding on condition) of soaking seed, pull seed out and after running water flushing, the seed that will wet is placed directly in negative electrode plate or the positive electricity pole plate. The DC electric field that then adds 100-500kV/m between pole plate continues 10-30 minutes. If use the mode of Fig. 1 to process seed, be called the positive electric field seed and process. If use the mode of Fig. 2 to process seed, then be called the negative electric field seed and process. Be disposed at every turn, with lucite or pvc material rod, its diameter is that 2-3 centimetre, length are 0.5-1 rice, and an end is 0.5-1W with wire serial connection power, resistance is that resistance and the earth of 500-800 ohm joins, and the other end is with holding discharging rod the moment short circuit of positive and negative electrode plate, to bleed off the electric charge on the pole plate, prevent electric shock, guarantee handling safety.
The present invention has been owing to adopted integrated conduct method, thereby reached germinations of sprouting in advance of promotion seed, improved germinate fast, germination percentage and seedling increment, reduced the effect of seed in the ground time of staying; Simultaneously, can effectively improve seedling quality and planting percent, such as table 1, such as table 2, such as table 3, such as table 5, as shown in table 6 such as table 4. Wherein, table 1 is to utilize 25ppm ABT immersion oil pine seed in the laboratory, through the sprouting result of positive electric field processing at 11 days. Table 2 is to utilize 50ppm GA immersion oil pine seed in the laboratory, processes 8 days sprouting results with positive electric field. Table 3 is to utilize 100ppm GA to soak European loose seed in the laboratory, processes 10 days sprouting results with negative electric field. Table 4 is to utilize 50ppm ABT immersion oil pine seed in the laboratory, processes the long result of the root of measuring afterwards seedling at 10 days with positive electric field. Table 5 is to sprinkle water in Chinese pine seeds, mixes Ca (OH)2Powder, with after the electric field treatment in mountain area, Danfeng County, Shaanxi sample prescription germination result. Table 6 is waterings and be mixed with Ca (OH) in Chinese pine seeds2Powder uses positive electric field 100kV/m 10 ' to process, and broadcasts the seedling result who distinguishes difference plant seeds by airplane masson pine and Chinese pine kind at temple, the Yuanshan mountain, Hanzhong and large mountain top. Two broadcast 800～1700 meters of district's height above sea level, 800～1000 millimeters of annual rainfalls. Masson pine seedling contrast is high by 67.3% then; In the situation of the minimizing amount of broadcasting 10%, treatment region Chinese pine then seedling contrast is high by 24.0%, and seedling increment contrast is high by 11.1%.
Table 1
| The test group | Average germination percentage (%) | With the contrast difference |
| 300kv/m 10 ' 300kv/m 20 ' 500kv/m 10 ' 500kv/m 20 ' control group | 57.0 55.7 61.7 60.3 52.7 | 4.3 3.0 9.0 ** 7.7 * 0 |
Table 1 is the impact (sprouting 11 days) on sprouting behind the DC electric field processing preimpregnation 25ppmABT Chinese pine seeds
Table 2
| The test group | Average germination percentage (%) | With the contrast difference |
| 500kv/m 10 ' 500kv/m 20 ' 500kv/m 30 ' control group | 40.7 38.0 35.7 22.0 | 18.7 ** 16.0 ** 13.7 ** 0 |
Influence (sprouting 8 days) to sprouting behind the table 2 DC electric field processing preimpregnation 50ppmGA Chinese pine seed
Table 3
| 6 days germination rates | 8 days germination rates | 10 days germination rates | Sprout index | Sprout time | |
| Control group-300kV/m 10 '-500kV/m 10 ' | ?41.7±5.7 ?54.7±4.7 ?54.0±17.4 | ?58.0±6.0 ?69.0±3.6 ?69.3±14 | ?68.33±6.7 ?78.33±4.2 ?79.0±9.17 | ?12.8±1.5 ?15.3±1.3 ?15.2±3.3 | ?6.1±0.5 ?5.7±0.2 ?5.9±0.6 |
Table 3 DC electric field is handled behind the loose seed in preimpregnation 100ppmPEG Europe the influence (sprouting 10 days) to germination rate
Table 4
| Experimental group | Average seedling root long (cm) | With the contrast difference |
| 500kv/m 10 ' 500kv/m 20 ' 500kv/m 30 ' control group | ?7.8 ?10.9 ?8.7 ?5.0 | 2.8 *5.9 **3.7 **0 |
Table 4 is influences (sprout 10 day) high to seedling behind the DC electric field processing preimpregnation 50ppmABT Chinese pine seed
Table 5
| Test | Sample prescription number (individual) | On average emerge and count (strain) | Average germination vigor (%) | Average germination percentage (%) | The germination potential difference | Germination rate increases |
| The electricity group | 20 | 515 | 19.9 | 51.5 | 6.5％ | 11.9％ |
| Control group | 20 | 396 | 13.4 | 39.6 |
Table 5 is that the Chinese pine seed is mixed with Ca (OH) through spreading water
2Behind the powder in Dan Fengxian mountain area, Shaanxi sample prescription germination test result.
Each sample prescription is broadcast 1000 in the table, 20 repetitions.After arranging sample prescription, observed once in per three days.Throw in continuous drought behind the seed, germination period continues 73 days, and the result shows: the processed group 5l5 strain of on average emerging, control group is 395 strains.
Table 6
| Test name | The plant seeds by airplane sortie | Plant seeds by airplane area (mu) | The amount of broadcasting jin/mu | The average strain number in sample garden | Average every mu of strain number | Average height of seedling (cm) | Leading thread (cm) | Seeds |
| Check plot, electric treatment district | ?2 ?1 | ?6390 ?3210 | ?0.5 ?0.5 | ?9.7 ?5.7 | ?637 ?380 | ?5.8 ?5.9 | ?0.1 ?0.1 | The masson pine masson pine |
| Electric treatment district check plot R～8 medicaments electricity+R8 medicament | ?1 ?1 ?1 ?1 | ?1530 ?1710 ?1710 ?1710 | ?0.63 ?0.7 ?0.7 ?0.7 | ?11 ?9 ?11 ?12 | ?744 ?600 ?734 ?800 | ?5.0 ?4.5 ?5.0 ?5.0 | ?0.2 ?0.2 ?0.2 ?0.2 | Chinese pine Chinese pine Chinese pine Chinese pine |
Table 6 is that Chinese pine, masson pine kind are mixed Ca (OH)
2Handle in Hanzhong area scattered seeds by airplane for afforestation result of the test with DC electric field.
When the data shown in the table 6 were at temple, the Yuanshan mountain, Hanzhong and the experiment of district difference plant seeds by airplane masson pine and Chinese pine kind is broadcast on the mountain top greatly, two broadcast 800～1700 meters of district's height above sea level, 800～1000 millimeters of annual rainfalls.Masson pine becomes the seedling comparison according to high by 67.3% then; Under the situation of the minimizing amount of broadcasting 10%, the treatment region Chinese pine is compared according to high by 24.0% when year's harvest seedling, and the comparison of growth of seedling amount is 11.1% according to height.
Embodiment 1: the positive direct-current electric field treatment was soaked the Chinese pine seed of (ABT) root-inducing powder: it is that its concentration of 1.5kg is in the root-inducing powder aqueous solution of 25ppm that the 0.5kg seed is put into weight, soaking seed 24 hours, after pulling out, is the field intensity of 300kV/m with dosage, handled 10 minutes, and recorded germination rate 57.0%; With dosage is the field intensity of 300kV/m, handles germination rate 55.7% 20 minutes; With dosage is the field intensity of 500kV/m, handles germination rate 61.7% 10 minutes; With dosage is the field intensity of 500kV/m, handles germination rate 60.7% 20 minutes.Contrast is 52.7%.
Embodiment 2: the positive direct-current electric field treatment was soaked the masson pine seed of (GA) gibberellin: it is that its concentration of 1.5kg is seed soaking 24 hours in the gibberellin aqueous solution of 50ppm (GA) that the 0.5kg seed is put into weight, after pulling seed out, with dosage is the 500kV/m field intensity, handled 10 minutes, and recorded germination rate 40.7%; With dosage is the field intensity of 500kV/m, handles germination rate 38.0% 20 minutes; With dosage is the field intensity of 500kV/m, handles germination rate 35.7% 30 minutes.Control group is 22.0%.
Embodiment 3: negative DC electric field is handled and soaked the European loose seed of (PEG) conditioning agent (extensive use in the world): it is that its concentration of 1kg is in 100ppm (PEG) the conditioning agent aqueous solution that the loose seed in 0.5kg Europe is put into weight, soaked seed 12 hours, after pulling out, with negative DC electric field, its dosage is-field intensity of 300kV/m, handled 10 minutes, and sprouted, record germination rate 78.3% through 10 days; With dosage is-field intensity of 500kV/m to handle germination rate 79.0% 10 minutes.Control group is 68.0%.
Implementation column 4: the positive direct-current electric field treatment was soaked the Chinese pine seed of composite fertilizer: 0.5kg Chinese pine seed was put in the 50ppm composite fertilizer aqueous solution of 1.5kg seed soaking 12 hours, after pulling seed out, with positive DC electric field, its dosage is the field intensity of 300kV/m, handled 10 minutes, sprouted the back and measure germination rate 49.0% in 10 days; With dosage is the field intensity of 500kV/m, handles germination rate 56.0% 10 minutes; With dosage is the field intensity of 700kV/m, handles germination rate 52.7% 10 minutes.Control group is 45.0%.
Embodiment 5: sample and plant seeds by airplane test is established in the mountain area: after being sprinkled into the running water of 80kg and mixing thoroughly in 800kg Chinese pine seed, mix 5%Ca (OH)
2Powder, spread 3 hours out after, with the field intensity of seed, handled 10 minutes with its dosage of positive direct-current electric field 100kV/m.Most of seed is by air seeding, and the processing of sampling and control sample are set to 20 processing and contrast respectively, and every side broadcasts 1000, through 73 sprouting test of Tianshan District sample prescription and observations, records processed group average germination percentage 51.5%, and control group is 39.6%.But, under laboratory condition, if add 1%Ca (OH)
2Powder is sprouted and is reduced.
Embodiment 6: scattered seeds by airplane for afforestation test: in the 1600kg of Hanzhong masson pine seed, be sprinkled into the running water of 160kg and mix thoroughly, then, evenly admix 3%Ca (OH) therein
2Powder, spread 3 hours out after, use the positive direct-current electric field, its dosage is the field intensity of 100kV/m, handles 10 minutes.After the plant seeds by airplane in spring, go into the winter then and investigate into seedling, every mu of average one-tenth seedling 637 strains of electric treatment district, the check plot is 380 strains.
Embodiment 7: scattered seeds by airplane for afforestation test: be sprinkled into the running water of 48kg and mix thoroughly in the 480kg of Hanzhong Chinese pine seed, then, evenly admix 1%Ca (OH) in seed
2Powder and vexed kind were used the positive direct-current electric field after 3 hours, and its dosage is the field intensity of 100kV/m, handles 10 minutes.After the plant seeds by airplane, go into winter one-tenth seedling investigation then, every mu of average one-tenth seedling 744 strains of electric treatment district, soaking water absorbing agent is check plot 600 strains.
Embodiment 8: scattered seeds by airplane for afforestation test: admix the R-8 repellent 10kg of popularization in the 598kg of Hanzhong Chinese pine kind, dry on request after mixing thoroughly.Then, use the positive direct-current electric field, its dosage is the field intensity of 100kV/m, handles 10 minutes.After the air seeding, go into winter one-tenth seedling investigation then, the R-8 district of electric treatment, 800 strains of every mu of average one-tenth seedling, the R-8 check plot is 734 strains, soaking water absorbing agent is check plot 600 strains.
Claims (5)
1. the method for comprehensive pretreatment seeds is characterized in that DC electric field is combined with routine techniques, at first adopts nutrient solution seed soaking or seed dressing, applies electric field to seed then, and concrete steps are:
1. using the nutritive solution of 25-100ppm, is 1 in seed with the solution weight ratio: 0.1-3 ratio doubly, and seed stirred soaked 3-24 hour, carry out preliminary treatment;
2. pretreated seed is put into plate electrode, applying electric-field intensity is 100-500kV/m, DC electric field, handled 10-30 minute, to change the saturating property of bio-electric field, ion concentration distribution, kind skin of seed, electrical conductivity is breathed and enzymic activity;
3. in the seed that electric field treatment is crossed, admix 1-5%Ca (OH)
2Powder is to increase immunising seed power.
2. method for treating seeds according to claim 1 is characterized in that nutrient solution, is ABT root-inducing powder, GA gibberellin, PEG conditioning agent, medicament and other any chemical element, trace element are dissolved in the solution of water composition.
3. method for treating seeds according to claim 1 is characterized in that using parallel-plate electrode, applies DC electric field, adopt positive polarity or negative polarity to handle to soak, spread any chemical element seed.
4. method for treating seeds according to claim 1, it is characterized in that adopting the seed dressing mode to handle prepreparing tree-seeds for aerosowing, calculate by per 100 kilograms of seeds, be spilled into 25-100ppm nutrient solution 5-15 kilogram, mix thoroughly and vexed kind 3-24 hour, then handled 10-30 minute with DC electric field parallel-plate electrode, the lead electrode of electric-field intensity 100-500kV/m.
5. method for treating seeds according to claim 1, it is characterized in that adopting the seed soaking mode to handle prepreparing tree-seeds for aerosowing, calculate by per 100 kilograms of seeds, be spilled into 25-100ppm nutrient solution 10-45 kilogram, soaking seed 3-24 hour, is that the DC electric field parallel-plate electrode, lead electrode of 100-500kV/m handled 10-30 minute with electric-field intensity again.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104025753A (en) * | 2014-05-16 | 2014-09-10 | 和县绿丰种业有限公司 | Suzhouqing seed germination accelerating method |
| RU2532043C1 (en) * | 2013-05-07 | 2014-10-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Поволжский государственный технологический университет" | Method of presowing treatment of scotch pine seeds |
| CN105359665A (en) * | 2015-11-02 | 2016-03-02 | 和县正林苗木专业合作社 | Method for weak current accelerating germination of huangguan pear trees |
| CN107135705A (en) * | 2017-05-25 | 2017-09-08 | 上海交通大学 | Seed priming processing method based on HEHA |
| CN108093737A (en) * | 2017-12-08 | 2018-06-01 | 桂林亦元生现代生物技术有限公司 | The method and its plant cultivating method of MEDULLA TETRAPANACIS sapling multiplication |
| CN108966738A (en) * | 2018-07-27 | 2018-12-11 | 贵州省茗之源种植专业合作社 | A kind of method for treating seeds preventing grey mould fruit rot of strawberry |
| CN110012827A (en) * | 2019-03-20 | 2019-07-16 | 广东东阳光药业有限公司 | A kind of serpentgrass method for culturing seedlings |
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2000
- 2000-12-07 CN CNB001356038A patent/CN1149010C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2532043C1 (en) * | 2013-05-07 | 2014-10-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Поволжский государственный технологический университет" | Method of presowing treatment of scotch pine seeds |
| CN104025753A (en) * | 2014-05-16 | 2014-09-10 | 和县绿丰种业有限公司 | Suzhouqing seed germination accelerating method |
| CN104025753B (en) * | 2014-05-16 | 2015-12-02 | 和县绿丰种业有限公司 | A kind of SUZHOUQING(sic) seed accelerating germination method |
| CN105359665A (en) * | 2015-11-02 | 2016-03-02 | 和县正林苗木专业合作社 | Method for weak current accelerating germination of huangguan pear trees |
| CN107135705A (en) * | 2017-05-25 | 2017-09-08 | 上海交通大学 | Seed priming processing method based on HEHA |
| CN108093737A (en) * | 2017-12-08 | 2018-06-01 | 桂林亦元生现代生物技术有限公司 | The method and its plant cultivating method of MEDULLA TETRAPANACIS sapling multiplication |
| CN108093737B (en) * | 2017-12-08 | 2021-02-26 | 桂林亦元生现代生物技术有限公司 | Method for propagating ricepaperplant pith seedlings and plant cultivation method thereof |
| CN108966738A (en) * | 2018-07-27 | 2018-12-11 | 贵州省茗之源种植专业合作社 | A kind of method for treating seeds preventing grey mould fruit rot of strawberry |
| CN110012827A (en) * | 2019-03-20 | 2019-07-16 | 广东东阳光药业有限公司 | A kind of serpentgrass method for culturing seedlings |
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| CN1149010C (en) | 2004-05-12 |
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