CN107135705A - Seed priming processing method based on HEHA - Google Patents
Seed priming processing method based on HEHA Download PDFInfo
- Publication number
- CN107135705A CN107135705A CN201710379471.1A CN201710379471A CN107135705A CN 107135705 A CN107135705 A CN 107135705A CN 201710379471 A CN201710379471 A CN 201710379471A CN 107135705 A CN107135705 A CN 107135705A
- Authority
- CN
- China
- Prior art keywords
- seed
- heha
- processing method
- electric field
- irradiation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to a kind of seed priming processing method based on HEHA, including damp and hot pretreatment seed, pending seed moisture content is lifted, then carries out standing after electric field treatment with irradiation, last withering step.Described pretreating method for seed and step are specific as follows：Soak seed 1 8h at 15 50 DEG C；In the electric field treatment with irradiation, electric field is high-voltage electrostatic field, and the condition for the treatment of with irradiation is：1 20kv/cm electric-field intensity, irradiates 1 60min.The present invention triggers the method being combined using damp and hot trigger with high-voltage electrostatic field, accelerate the overall physiology course of seed sprouting, activationary time is foreshortened within 3 17h, and activation effect is embedded by seed re-dry, the purpose for improving percentage of seedgermination and regularity is reached.After HEHA triggers, the vigor of seed is obviously improved, and triggers effect under normal the storage of seeds condition (5 DEG C), can be maintained for more than year.
Description
Technical field
The present invention relates to agricultural technology field, and in particular to seed treatment and Seed germination techniques field, more particularly to one
Plant the seed priming processing method based on HEHA (Hydro-Electricfield Hybrid Activation).
Background technology
Seed is the most basic means of production in agricultural production, and seed quality has very big shadow to the yield and quality of crop
Ring, seed vitality is to weigh the reliability index of seed quality.Crop seed often because of the production of hybrid seeds when maturity is inconsistent, Storing-year mistake
Length is in deep sleep state and causes bud ratio too low or even disappear, so as to influence germination and quality of emerging, and shows
Requirement more and more higher for agricultural production to the germination percentage of seed, emergence rate, speed of emergence and Phase ofsprouting.Seed enterprises
Every year substantial amounts of seed is scrapped because bud ratio is unable to reach national standard.According to incompletely statistics, what China scrapped every year is various
Vegetable seeds total amount causes more than 40,000,000 yuan of direct economic loss more than 200 tons, about.This is not only caused to Seed enterprises
Great economic loss, also influences normal agricultural production order because of seed shortage, aggravates peasant economy burden.Research card
Bright, the vigor of seed can be by some processing and be recovered or be improved, before numerous broadcast in method for treating seeds, kind
It is a kind of effective way for recovering seed vitality that son, which triggers,.Traditional elicitation technique, which has, damp and hot to be triggered, oozes and regulate and guide hair, matrix and draw
Hair, chemistry initiation etc..The principle of these technologies is all that seed is placed in the liquid environment of certain osmotic pressure it is slowly absorbed water
To a certain degree, recycle the biologos of seeds self to carry out the reparation of the organelles such as cell membrane, DNA, and remove free radical,
Active oxygen, abscisic acid etc. press down biomass, so as to improve the constitution and vigor of seed.The initiation effect of these elicitation techniques can be kept
More than year, but trigger the operating time longer (12-120 hours), and in the critical moisture content to germination
Control difficulty larger, the cost that oozing needed for triggering adjusts liquid, matrix, chemical reagent etc. to trigger consumptive material is higher, and configuration difficulty is larger.
Electric field, which triggers, (2-60 minutes) to be obviously improved the activity of seed sprouting key enzyme within the extremely short time, so as to carry
Seed vitality is risen, but the duration for the treatment of effect is shorter (1-4 weeks).
The content of the invention
There is provided a kind of seed priming processing method based on HEHA for defect of the invention for prior art.The present invention
Will using HEHA [(Hydro-Electricfield Hybrid Activation), damp and hot-electric field seed mixture activation] technology
The damp and hot Dominant Facies with two kinds of elicitation techniques of electric field are combined, the effect triggered using the operating procedure embedding electric field of damp and hot elicitation technique
Really, make the long-term effect of its existing damp and hot initiation, the initiation time is highly shortened again, and improve germinating energy and the germination of seed
Rate.
The purpose of the present invention is achieved through the following technical solutions：
The invention provides a kind of seed for being based on HEHA (Hydro-Electricfield Hybrid Activation)
Trigger processing method, including pretreatment seed, to lift pending seed relative water content, then carry out electric field treatment with irradiation,
Last withering step.
Preferably, the step of pretreatment seed specifically uses following methods：Soaked seed at 15-30 DEG C 1-8 hour.
It is described it is too high or too low for temperature can reduce later stage electric field initiation effect.
Preferably, it is 10-50% that the seed, which carries out pretreated water content,.
Preferably, in the electric field treatment with irradiation, electric field is high-voltage electrostatic field, and the condition for the treatment of with irradiation is：1-20kv/cm
High-voltage electrostatic field irradiation 1-60min.Electricity of amylase, protease and the lipase related to seed sprouting in 1-20kv/cm
Its three-level, quaternary structure are in the change of positive effect under field intensity, and enzymatic activity is improved；Electric-field intensity is less than this scope then
The structure of enzyme can not be caused to change, inactive raising effect；Damage then is produced to structure higher than this scope, causes enzymatic activity
Reduction.
Preferably, the processing method, which is additionally included in after electric field treatment with irradiation, carries out stewing process；The stewing process
Condition is：Environment relative air humidity 90-99% is stood, 15-28 DEG C of environment temperature, time of repose 2-9h is stood.
Preferably, the condition of the drying process is：40-45 DEG C of drying temperature, drying time 30-180min.
Preferably, seed moisture content is 5-10% after the drying.
Compared with prior art, the present invention has following beneficial effect：
The present invention is mutually tied being relatively easy to the initiation of damp and hot initiation leading portion and high-voltage electrostatic field using by seed moisture control
Close, the physiological activity related to seed sprouting is started by damp and hot initiation leading portion, and sprouted with seed by high-voltage electrostatic field raising
The activity of related enzyme is sent out, accelerates various gene expressions, makes relevant enzyme in the common lifting of quantity and activity, accelerates cell membrane, DNA
The removing of the suppression biomass such as reparation and free radical, active oxygen, abscisic acid Deng organelle, so that the entirety for accelerating seed to sprout is raw
Reason process, activationary time is foreshortened within 2-10h, and reaches final draw by seed re-dry to embed activation effect
Send out purpose.Seed after HEHA triggers, it triggers effect to be maintained for more than one under normal the storage of seeds condition (5 DEG C)
Year time, and the germination percentage of seed is obviously improved.
Brief description of the drawings
By reading the detailed description made with reference to the following drawings to non-limiting example, further feature of the invention,
Objects and advantages will become more apparent upon：
Fig. 1 is the schematic diagram that high-voltage electrostatic field seed priming processing equipment is set up；
Wherein：1st, electric field positive plate；2nd, electric field negative plate；3rd, seed；4th, direction of an electric field；5th, field generator power supply；6、
Earth atmosphere direction of an electric field.
Embodiment
With reference to specific embodiment, the present invention is described in detail.Following examples will be helpful to the technology of this area
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that to the ordinary skill of this area
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
Embodiment 1
Present embodiments provide a kind of onion seed based on HEHA and trigger processing method, using high pressure as shown in Figure 1
Electrostatic field seed priming processing equipment component, including electric field positive plate 1, electric field negative plate 2, seed 3, direction of an electric field 4, electric field hair
Raw device power supply 5, earth atmosphere direction of an electric field 6.
Comprise the following steps that：
" Supreme Being is yellow " onion seed is used (to originate in 2014, natural aging 1 year, bud rate 75% under glasshouse environment.) enter
Row HEHA mixing triggers.Onion seed is soaked seed in 25 DEG C of warm water after 4h, takes out and drain to remove the surface of the seed residual moisture；
Be placed in 5-15kv/cm high-voltage electrostatic field and handle after seed 10-60min, by seed be positioned over 98% relative humidity, 25 DEG C
9h is stood in growth cabinet.Using 43 DEG C of oven drying seed 30-180min, 5-10% is dropped to its relative water content.
The dry seed of completion is placed under room temperature environment is seeded in culture dish progress Seed germination after 7d：Culture dish is positioned over artificial gas
Wait in case, maintain 25 DEG C of room temperature and humidity 90%.It is 97% that bud rate is measured after 10d, illustrates that HEHA mixing elicitation technique can show
Write the bud ratio for the onion seed for recovering natural aging.
High-quality " Supreme Being yellow " onion seed (is originated in 2014,5 DEG C, store under 10% relative humidity conditions, bud rate is
95%) high-quality " Supreme Being the is yellow " onion seed and triggered through HEHA is positioned in 45 DEG C of growth cabinets with humidity 90% of room temperature
7d, carries out manual simulation's Seed Aging.Above-mentioned Seed germination is repeated, and measures the high-quality onion seed 10d after burin-in process
When bud ratio deteriorate to 79%, and bud ratio of the high-quality onion seed triggered through HEHA after burin-in process is 86%, is said
Bright HEHA mixing elicitation technique can significantly inhibit influence of the onion seed aging to its bud ratio.By determining, after planting
24h, aging onion seed SOD (superoxide dismutase) activity triggered by HEHA reaches peak 414.219U/g, high
In without the 23.8% of initiation seed.
Embodiment 2
A kind of crowndaisy chrysanthemum seed priming processing method based on HEHA is present embodiments provided, is comprised the following steps that：
(originated in 2016, bud rate 60%, 5 DEG C, under 10% relative humidity conditions using high-quality " fragrant chrysanthemum No. three " crowndaisy chrysanthemum seed
Storage) carry out HEHA mixing initiations.Crowndaisy chrysanthemum seed is soaked seed in 25 DEG C of warm water after 2h, and seed is taken out and drained to remove seed
Remained on surface moisture.After 5-15kv/cm high-voltage electrostatic field processing seed 10-60min, seed is positioned over 98% relatively wet
9h is stood in degree, 25 DEG C of growth cabinet.Using 43 DEG C of oven drying seeds, 5-10% is dropped to its relative water content.
The dry seed of completion is placed under room temperature environment is seeded in culture dish progress Seed germination after 7d.Culture dish is positioned over artificial gas
Wait in case, it is 71% to maintain to measure bud ratio after 25 DEG C of room temperature and humidity 90%, 10d, illustrate that HEHA mixing elicitation techniques can
It is obviously improved the bud ratio of the crowndaisy chrysanthemum seed of high-quality.High-quality " fragrant chrysanthemum No. three " crowndaisy chrysanthemum seed (is originated in 2016, bud rate is
60%, 5 DEG C, store under 10% relative humidity conditions) be positioned over completing high-quality " fragrant chrysanthemum No. three " crowndaisy chrysanthemum seed of HEHA initiations
7d in 45 DEG C of growth cabinets with humidity 90% of room temperature, carries out manual simulation's Seed Aging.Above-mentioned Seed germination is repeated, and is surveyed
High-quality crowndaisy chrysanthemum seed bud rate that must be after burin-in process deteriorates to 49%, and the aging crowndaisy chrysanthemum seed bud rate triggered through HEHA is
63%, illustrate that HEHA mixing elicitation technique can significantly inhibit influence of the crowndaisy chrysanthemum Seed Aging to its bud ratio.By determining, broadcast
24h after kind, crowndaisy chrysanthemum seed SOD (superoxide dismutase) activity triggered by HEHA reaches peak 486.771U/g, high
In without the 42.6% of initiation seed.
Comparative example 1
Present embodiments provide a kind of onion seed based on HEHA and trigger processing method, comprise the following steps that：
" Supreme Being is yellow " onion seed (originating in 2014, natural aging 1 year, bud rate 75% under glasshouse environment) is used to carry out
Conventional water triggers, i.e., respectively at after seed soaking 4h, 24h, 72h in 25 DEG C of warm water, by seed be positioned over 98% relative humidity, 25 DEG C
9h in growth cabinet.Using 43 DEG C of oven drying seeds, 5-10% is dropped to its relative water content.Complete dry seed
Placed under room temperature environment and culture dish progress Seed germination is seeded in after 7d.Culture dish is positioned in growth cabinet, maintains room
25 DEG C of temperature and humidity 90%.It is respectively 81%, 82%, 73% that bud rate is measured after 10d.
Comparative example 2
Present embodiments provide a kind of onion seed based on HEHA and trigger processing method, comprise the following steps that：
" Supreme Being is yellow " onion seed (originating in 2014, natural aging 1 year, bud rate 60% under glasshouse environment) is used to carry out
Conventional electric field triggers, i.e., with 5-15kv/cm high-voltage electrostatic field processing seed 10-60min.The seed of electric field initiation is completed in room
Placed under warm environment and culture dish progress Seed germination is seeded in after 7d.Culture dish is positioned in growth cabinet, maintains room temperature 25
DEG C with humidity 90%.It is 87% that bud rate is measured after 10d.
Concrete application approach of the present invention is a lot, and described above is only the preferred embodiment of the present invention.It should be pointed out that more than
Embodiment is merely to illustrate the present invention, and the protection domain being not intended to limit the invention.For the common skill of the art
For art personnel, under the premise without departing from the principles of the invention, some improvement can also be made, these improvement also should be regarded as this hair
Bright protection domain.
Claims (7)
1. one kind is based on HEHA seed priming processing methods, it is characterised in that including pretreatment seed, then carry out electric field irradiation
Processing, last withering step.
2. the seed priming processing method according to claim 1 based on HEHA, it is characterised in that the pretreatment seed
The step of specifically use following methods：Soaked seed at 15-50 DEG C 1-8 hour.
3. the seed priming processing method according to claim 1 or 2 based on HEHA, it is characterised in that the seed is pre-
Relative water content after processing is 10-50%.
4. the seed priming processing method according to claim 1 based on HEHA, it is characterised in that at the electric field irradiation
In reason, electric field is high-voltage electrostatic field, and the condition for the treatment of with irradiation is：1-20kv/cm high-voltage electrostatic field irradiation 1-60min.
5. the seed priming processing method according to claim 1 based on HEHA, it is characterised in that the processing method is also
It is included in after electric field treatment with irradiation and carries out stewing process；The condition of the stewing process is：Environment relative air humidity 90-
99%, 15-28 DEG C of environment temperature, time of repose 2-9h.
6. the seed priming processing method according to claim 1 based on HEHA, it is characterised in that the drying process
Condition is：40-45 DEG C of drying temperature, drying time 30-180min.
7. the seed priming processing method according to claim 1 based on HEHA, it is characterised in that seed after the drying
Water content is 5-10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710379471.1A CN107135705A (en) | 2017-05-25 | 2017-05-25 | Seed priming processing method based on HEHA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710379471.1A CN107135705A (en) | 2017-05-25 | 2017-05-25 | Seed priming processing method based on HEHA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107135705A true CN107135705A (en) | 2017-09-08 |
Family
ID=59779795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710379471.1A Pending CN107135705A (en) | 2017-05-25 | 2017-05-25 | Seed priming processing method based on HEHA |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107135705A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031168A (en) * | 1987-08-14 | 1989-02-22 | 鞍山市静电技术研究所 | Apparatus for treating seeds by electrostatic field |
| CN1295785A (en) * | 2000-12-07 | 2001-05-23 | 西安电子科技大学 | Method for treating seed in ac electric field |
| JP2007117066A (en) * | 2005-09-30 | 2007-05-17 | Techno Giken Kk | Method for physically treating plant seed, and methods for breeding and growing seedling |
| CN102577691A (en) * | 2011-11-08 | 2012-07-18 | 西安理工大学 | System using extremely low frequency pulsed electrical field to treat seeds and treatment method |
| CN103109619A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Osmoregulation initiation method of cotton seeds under low-temperature stress |
-
2017
- 2017-05-25 CN CN201710379471.1A patent/CN107135705A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031168A (en) * | 1987-08-14 | 1989-02-22 | 鞍山市静电技术研究所 | Apparatus for treating seeds by electrostatic field |
| CN1295785A (en) * | 2000-12-07 | 2001-05-23 | 西安电子科技大学 | Method for treating seed in ac electric field |
| JP2007117066A (en) * | 2005-09-30 | 2007-05-17 | Techno Giken Kk | Method for physically treating plant seed, and methods for breeding and growing seedling |
| CN102577691A (en) * | 2011-11-08 | 2012-07-18 | 西安理工大学 | System using extremely low frequency pulsed electrical field to treat seeds and treatment method |
| CN103109619A (en) * | 2012-11-16 | 2013-05-22 | 高润宝 | Osmoregulation initiation method of cotton seeds under low-temperature stress |
Non-Patent Citations (6)
| Title |
|---|
| 吴敏 等: ""种子吸湿-回干处理研究综述"", 《种子》 * |
| 周盛祥 等: ""高压静电场处理种子研究现状与发展对策"", 《农业工程》 * |
| 徐伟忠 等: "《物理农业》", 31 August 2016, 解放军出版社 * |
| 沈颖 等: ""蔬菜种子处理技术研究进展"", 《中国种业》 * |
| 邓红梅 等: ""高压静电场处理干湿黄瓜种子的生物效应及机理"", 《农机化研究》 * |
| 鲍重光 主编: "《现代静电技术》", 31 October 1988, 万国学术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102487627B (en) | Method for effectively breaking dormancy of Paris polyphylla seeds | |
| CN102002474B (en) | Rapid bulb multiplication method by using fritillaria cirrhosa leaves as explant | |
| CN105027733B (en) | A kind of method for promoting Germination of Taxus mairei Seeds | |
| CN103609223A (en) | Method Paris polyphylla var.chinensis through gibberellin and wet sand stratification | |
| CN101663934B (en) | Regulating and controlling method for improving germination rate of woody seeds | |
| CN103444309B (en) | Gibberellin and filter paper combine the method making paris polyphylla seed quick-speed germination | |
| CN104982116B (en) | The germination accelerating method of gingko seedses | |
| CN109258426B (en) | Efficient seedling raising method for stachys sieboldii seeds | |
| CN107135705A (en) | Seed priming processing method based on HEHA | |
| CN107278414A (en) | A kind of method for promoting rare or endangered species RHIZOMA ET RADIX VALERIANAE JATNANSI seed to sprout | |
| CN103460855B (en) | Method for facilitating germination of sassafras seeds | |
| CN103283604B (en) | Method for removing endophytic bacteria of explants in lily tissue culture | |
| CN105144912B (en) | A kind of method that largeflower-like honeysuckle flower seed is sprouted | |
| CN104472055A (en) | Method for improving germination rate of xanthoceras sorbifolia bunge seeds by utilizing rare earth elements | |
| CN105123018B (en) | The germination accelerating method of Chinese yew seed | |
| CN103141177A (en) | Hard seed processing method | |
| CN103529037B (en) | A kind of Chinese pistache plant sex appraisal method | |
| CN106305113A (en) | Planting method capable of improving onion germination rate | |
| CN102845513A (en) | Method for accelerating wound healing of sweet potatoes | |
| CN102792803B (en) | Method for promoting germination of seeds of Tulipa edulis (Miq.) Baker | |
| CN113179704A (en) | Method for improving germination rate of Chinese medicinal material astragalus seeds | |
| CN207653092U (en) | A kind of jujube tree seedling culture slot | |
| CN106856709B (en) | Method for rapid germination of acer sanguinea seeds and seedling raising method | |
| CN103718699A (en) | Flowering dogwood germination accelerating method | |
| CN103931477A (en) | Novel acacia melanoxylon tissue culture seedling rooting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |