CN105123018B - The germination accelerating method of Chinese yew seed - Google Patents
The germination accelerating method of Chinese yew seed Download PDFInfo
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- CN105123018B CN105123018B CN201510454662.0A CN201510454662A CN105123018B CN 105123018 B CN105123018 B CN 105123018B CN 201510454662 A CN201510454662 A CN 201510454662A CN 105123018 B CN105123018 B CN 105123018B
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Abstract
The present invention provides a kind of germination accelerating method of Chinese yew seed, including：Chinese yew seed is carried out disinfection processing；Chinese yew seed is soaked with Gibberellins solution；The rice bran of 50 parts by weight is mixed with the sawdust of 50 parts by weight, and is mixed into fermenting agent, piles after bar pile ferments 57 days, Chinese yew seed is embedded in bar pile；Vermiculite is soaked with Gibberellins solution, is then charged into a polybag, is also placed in after Chinese yew the surface of the seed is polished with emery cloth in polybag, by plastic bag sealing, the polybag was rubbed once every 23 hours；By Chinese yew seed sponge wrapping, and soaked sponge with heteroauxin solution, it is then placed in pressurized tank, is kept for 15 20 minutes under the conditions of 8MPa, 20 DEG C, then keep 10 15 minutes, finally kept for 10 15 minutes under the conditions of 1MPa, 40 DEG C under the conditions of 5MPa, 30 DEG C.The present invention can break the dormancy of Chinese yew seed, improve germination percentage.
Description
Technical field
The present invention relates to Chinese yew artificial culture field.It is more particularly related to the vernalization side of Chinese yew seed
Method.
Background technology
Containing anti-cancer active matters such as taxols in the branches and leaves of Chinese yew, bark, with good medicinal Development volue and
Economic value.For the abundance for ensureing taxol and stable supply, the destruction to wild red bean China fir is also reduced, it is necessary to carry out red bean
The artificial culture of China fir.
But, the rest period of Chinese yew seed is longer, needs the summer in two winter one to sprout under field conditions (factors), a large amount of seeds
The meeting devitalization within rest period, thus directly sowed with Chinese yew seed, germination percentage is very low, also limits the people of Chinese yew
Work is cultivated.Therefore, need badly and design a kind of dormancy for abolishing Chinese yew seed, promote Chinese yew germination, improve Chinese yew kind
The method of sub- germination percentage.
The content of the invention
It is an object of the invention to solve at least the above and/or defect, and provide at least will be described later excellent
Point.
It is a still further object of the present invention to provide a kind of germination accelerating method of Chinese yew seed, it can break Chinese yew seed
Dormancy, improve the germinating energy of Chinese yew seed, improve germination percentage.
In order to realize that there is provided a kind of vernalization side of Chinese yew seed according to object of the present invention and further advantage
Method, comprises the following steps：
Soaked 20-40 seconds Step 1: Chinese yew seed is put into the mercuric chloride solution that mass fraction is 0.1%, Ran Houyong
Water is cleaned 3-5 times, then Chinese yew seed mass fraction is soaked 10-15 minutes for 0.1% liquor potassic permanganate, Ran Houyong
Water is cleaned 4-6 times；
Step 2: Gibberellins solution immersion 24-48 of the Chinese yew seed mass fraction that step one is obtained for 0.3%
Hour；
Step 3: the rice bran of 50 parts by weight is mixed with the sawdust of 50 parts by weight, the zymophyte of 1-2 parts by weight is then mixed into
Agent, piles bar pile and is fermented, after fermenting 5-7 days, and the Chinese yew seed that step 2 is obtained is embedded in bar pile, is taken after 5-7 days
Go out；Wherein, fermenting agent by bacillus subtilis, brown sugar and water with 1:1-1.5:10-15 weight ratio is mixed；
Step 4: taking vermiculite, soaked with mass fraction for 0.05% Gibberellins solution, be then charged into a polybag
In, the Chinese yew the surface of the seed that step 3 is obtained is also placed in polybag after being polished 3-5 minutes with emery cloth, and is mixed with vermiculite
Uniformly, plastic bag sealing was rubbed into the polybag once every 2-3 hours with rope, rubs every time 5-10 minutes, rub 5-8
Chinese yew seed is taken out after secondary；
Step 5: step 4 is obtained into each Chinese yew seed uses sponge wrapping, and the Yin for being 80mg/L with concentration
Indolylbutyric acid solution soaks sponge, is then placed in pressurized tank, is kept for 15-20 minutes, then existed under the conditions of 8MPa, 20 DEG C
5MPa, keep 10-15 minute under the conditions of 30 DEG C, finally holding 10-15 minutes under the conditions of 1MPa, 40 DEG C.
Preferably, the germination accelerating method of described Chinese yew seed, before the step one, in addition to：
Chinese yew seed is placed in clear water, after standing 10-20 minutes, the Chinese yew seed floated is removed, taking-up sinks to
Water-bed Chinese yew seed, dries.
Preferably, the germination accelerating method of described Chinese yew seed, in the step one, matter is put into by Chinese yew seed
Fraction is measured to be soaked 30 seconds in 1% mercuric chloride solution, Chinese yew seed mass fraction is soaked for 0.1% liquor potassic permanganate
Bubble 12 minutes.
Preferably, the germination accelerating method of described Chinese yew seed, in the step 2, Chinese yew seed and quality point
The weight ratio for the Gibberellins solution that number is 0.3% is 1:17, soak time is 36 hours.
Preferably, the germination accelerating method of described Chinese yew seed, in the step 3, the cross section of the bar pile is
Isosceles trapezoid, the height of the bar pile is 1 meter, and Chinese yew seed is embedded to the depth of the 0.4-0.6 rice of bar pile.
Preferably, the germination accelerating method of described Chinese yew seed, in the step 3, the weight for the fermenting agent being mixed into
It is 1.2 parts to measure part, and fermenting agent is by bacillus subtilis, brown sugar and water with 1:1.2:12 weight ratio is mixed.
Preferably, the germination accelerating method of described Chinese yew seed, in the step 4, the average grain diameter of vermiculite is 5-
8 millimeters, the weight ratio of vermiculite and Chinese yew seed is 13:1.
The present invention at least includes following beneficial effect：
(1) present invention is stimulated by plant hormone, biological thermostimulation, mechanical stimulus and alternating temperature transformation stimulation be in red bean
After China fir seed, the dormancy of Chinese yew seed can be abolished, the germination percentage of Chinese yew seed is improved to 90-95% by 40-50%.
(2) step one of the invention carries out sterilizing with mercuric chloride and liquor potassic permanganate to Chinese yew seed, it is to avoid red
Beans China fir seed is mouldy by courses of infection, rotten, and the soak time of potassium permanganate was advisable with 10-15 minutes, more than 15 minutes easily
Cause Chinese yew Interior Seed to be oxidized destruction, cause seed dead.
(3) step two of the invention soaks Chinese yew seed with 0.3% Gibberellins solution, is stimulated by plant hormone
Seed is sprouted, and tests below finding soak time at 24, and the germination percentage of Chinese yew seed increases very fast, 24-36 hours, germination
Rate increasess slowly, but when more than 36 small, it is impossible to further improve germination percentage.
(4) step three of the invention stimulates Chinese yew seed with the biological heat of rice bran fermentation generation, and fermentation is produced
Ferment also can further promote the metabolism of Chinese yew seed so that promote Chinese yew seed sprout.When fermentation 5-7 days
Afterwards, the temperature inside bar pile can reach 40-50 degree, and the amount of the ferment produced is more, and Chinese yew seed now is embedded into bar
Pile can effective stimulus Chinese yew seed sprout, the time buried of Chinese yew seed was advisable with 3-5 days, more than 5 days, in bar pile
The temperature in portion can be very high, and seed can be made dead.
(5) step four of the invention makes vermiculite friction Chinese yew seed, that is, uses mechanical stimulus red bean by rubbing polybag
China fir seed is sprouted, and gauze polishing and vermiculite friction make the kind skin of Chinese yew seed thinning, and the gibberellin in such vermiculite holds
Easily enter Interior Seed, it is possible to effective stimulus Chinese yew seed.Experiment finds that certain interval of time is just, it is necessary to rub once
The sprouting of Chinese yew seed can be effectively facilitated, interval time was advisable with 2-3 hours.
(6) step five of the invention is further promoted by carrying out pressure and temperature varying stimulation to the Chinese yew seed in pressurized tank
Enter the sprouting of Chinese yew seed.When pressure is 8MPa, temperature is preferably 20 DEG C, and is kept for 15-20 minutes, when pressure is 5MPa,
Temperature is preferably 30 DEG C, and is kept for 10-15 minutes, and when pressure is 1MPa, temperature is preferably 40 DEG C, and is kept for 10-15 minutes, is so dropped
Pressure heating, is capable of the metabolism of optimal stimulation Chinese yew seed, improves the germination percentage of Chinese yew seed, pressure no more than
8MPa, easily bad influence is caused to Chinese yew seed more than 8MPa.In addition, at a higher pressure, the indoles second in sponge
Acid can also enter Chinese yew Interior Seed, further promote Chinese yew seed to sprout.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to example, the present invention is described in further detail, to make those skilled in the art with reference to specification text
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or many
The presence or addition of individual other elements or its combination.
<Example 1>
A kind of germination accelerating method of Chinese yew seed, comprises the following steps：
Soaked 20 seconds Step 1: Chinese yew seed is put into the mercuric chloride solution that mass fraction is 0.1%, it is then clear with water
Wash 3 times, then Chinese yew seed mass fraction is soaked 10 minutes for 0.1% liquor potassic permanganate, then clean 4 with water
It is secondary；
Step 2: the Chinese yew seed mass fraction that step one is obtained is small for 0.3% Gibberellins solution immersion 24
When；
Step 3: the rice bran of 50 parts by weight is mixed with the sawdust of 50 parts by weight, the zymophyte of 1 parts by weight is then mixed into
Agent, piles bar pile and is fermented, after fermenting 5 days, and the Chinese yew seed that step 2 is obtained is embedded in bar pile, is taken out after 5 days；Its
In, fermenting agent is by bacillus subtilis, brown sugar and water with 1:1:10 weight ratio is mixed；
Step 4: taking vermiculite, soaked with mass fraction for 0.05% Gibberellins solution, be then charged into a polybag
In, the Chinese yew the surface of the seed that step 3 is obtained is also placed in polybag after being polished 3 minutes with emery cloth, and is mixed with vermiculite
It is even, with rope by plastic bag sealing, the polybag was rubbed once every 2 hours, is rubbed every time 5 minutes, rubs and takes out red after 5 times
Beans China fir seed；
Step 5: step 4 is obtained into each Chinese yew seed uses sponge wrapping, and the Yin for being 80mg/L with concentration
Indolylbutyric acid solution soaks sponge, is then placed in pressurized tank, under the conditions of 8MPa, 20 DEG C keep 15 minutes, then 5MPa,
Keep 10 minutes, finally kept for 10 minutes under the conditions of 1MPa, 40 DEG C under the conditions of 30 DEG C.
The germination accelerating method of described Chinese yew seed, before the step one, in addition to：
Chinese yew seed is placed in clear water, after standing 10 minutes, the Chinese yew seed floated is removed, taking-up sinks to the bottom
Chinese yew seed, dry.
The germination accelerating method of described Chinese yew seed, in the step 2, Chinese yew seed is 0.3% with mass fraction
Gibberellins solution weight ratio be 1:17.
The germination accelerating method of described Chinese yew seed, in the step 3, the cross section of the bar pile is isosceles trapezoid,
The height of the bar pile is 1 meter, and Chinese yew seed is embedded to 0.4 meter of depth of bar pile.
The germination accelerating method of described Chinese yew seed, in the step 4, the average grain diameter of vermiculite is 5-8 millimeters, leech
The weight ratio of stone and Chinese yew seed is 13:1.
<Example 2>
A kind of germination accelerating method of Chinese yew seed, comprises the following steps：
Soaked 40 seconds Step 1: Chinese yew seed is put into the mercuric chloride solution that mass fraction is 0.1%, it is then clear with water
Wash 5 times, then Chinese yew seed mass fraction is soaked 15 minutes for 0.1% liquor potassic permanganate, then clean 6 with water
It is secondary；
Step 2: the Chinese yew seed mass fraction that step one is obtained is small for 0.3% Gibberellins solution immersion 48
When；
Step 3: the rice bran of 50 parts by weight is mixed with the sawdust of 50 parts by weight, the zymophyte of 2 parts by weight is then mixed into
Agent, piles bar pile and is fermented, after fermenting 7 days, and the Chinese yew seed that step 2 is obtained is embedded in bar pile, is taken out after 7 days；Its
In, fermenting agent is by bacillus subtilis, brown sugar and water with 1:1.5:15 weight ratio is mixed；
Step 4: taking vermiculite, soaked with mass fraction for 0.05% Gibberellins solution, be then charged into a polybag
In, the Chinese yew the surface of the seed that step 3 is obtained is also placed in polybag after being polished 5 minutes with emery cloth, and is mixed with vermiculite
It is even, with rope by plastic bag sealing, the polybag was rubbed once every 3 hours, is rubbed every time 10 minutes, rubs and is taken out after 8 times
Chinese yew seed；
Step 5: step 4 is obtained into each Chinese yew seed uses sponge wrapping, and the Yin for being 80mg/L with concentration
Indolylbutyric acid solution soaks sponge, is then placed in pressurized tank, under the conditions of 8MPa, 20 DEG C keep 20 minutes, then 5MPa,
Keep 15 minutes, finally kept for 15 minutes under the conditions of 1MPa, 40 DEG C under the conditions of 30 DEG C.
The germination accelerating method of described Chinese yew seed, before the step one, in addition to：
Chinese yew seed is placed in clear water, after standing 20 minutes, the Chinese yew seed floated is removed, taking-up sinks to the bottom
Chinese yew seed, dry.
The germination accelerating method of described Chinese yew seed, in the step 2, Chinese yew seed is 0.3% with mass fraction
Gibberellins solution weight ratio be 1:17.
The germination accelerating method of described Chinese yew seed, in the step 3, the cross section of the bar pile is isosceles trapezoid,
The height of the bar pile is 1 meter, and Chinese yew seed is embedded to 0.6 meter of depth of bar pile.
The germination accelerating method of described Chinese yew seed, in the step 4, the average grain diameter of vermiculite is 5-8 millimeters, leech
The weight ratio of stone and Chinese yew seed is 13:1.
<Example 3>
A kind of germination accelerating method of Chinese yew seed, comprises the following steps：
Soaked 30 seconds Step 1: Chinese yew seed is put into the mercuric chloride solution that mass fraction is 0.1%, it is then clear with water
Wash 4 times, then Chinese yew seed mass fraction is soaked 12 minutes for 0.1% liquor potassic permanganate, then clean 5 with water
It is secondary；
Step 2: the Chinese yew seed mass fraction that step one is obtained is small for 0.3% Gibberellins solution immersion 36
When；
Step 3: the rice bran of 50 parts by weight is mixed with the sawdust of 50 parts by weight, the zymophyte of 1.2 parts by weight is then mixed into
Agent, piles bar pile and is fermented, after fermenting 6 days, and the Chinese yew seed that step 2 is obtained is embedded in bar pile, is taken out after 6 days；Its
In, fermenting agent is by bacillus subtilis, brown sugar and water with 1:1.2:12 weight ratio is mixed；
Step 4: taking vermiculite, soaked with mass fraction for 0.05% Gibberellins solution, be then charged into a polybag
In, the Chinese yew the surface of the seed that step 3 is obtained is also placed in polybag after being polished 4 minutes with emery cloth, and is mixed with vermiculite
It is even, with rope by plastic bag sealing, the polybag was rubbed once every 2 hours, is rubbed every time 8 minutes, rubs and takes out red after 7 times
Beans China fir seed；
Step 5: step 4 is obtained into each Chinese yew seed uses sponge wrapping, and the Yin for being 80mg/L with concentration
Indolylbutyric acid solution soaks sponge, is then placed in pressurized tank, under the conditions of 8MPa, 20 DEG C keep 18 minutes, then 5MPa,
Keep 12 minutes, finally kept for 13 minutes under the conditions of 1MPa, 40 DEG C under the conditions of 30 DEG C.
The germination accelerating method of described Chinese yew seed, before the step one, in addition to：
Chinese yew seed is placed in clear water, after standing 15 minutes, the Chinese yew seed floated is removed, taking-up sinks to the bottom
Chinese yew seed, dry.
The germination accelerating method of described Chinese yew seed, in the step 2, Chinese yew seed is 0.3% with mass fraction
Gibberellins solution weight ratio be 1:17.
The germination accelerating method of described Chinese yew seed, in the step 3, the cross section of the bar pile is isosceles trapezoid,
The height of the bar pile is 1 meter, and Chinese yew seed is embedded to 0.5 meter of depth of bar pile.
The germination accelerating method of described Chinese yew seed, in the step 4, the average grain diameter of vermiculite is 5-8 millimeters, leech
The weight ratio of stone and Chinese yew seed is 13:1.
In order to illustrate the effect of the present invention, it is as follows that inventor provides comparative experiments：
<Comparative example 1>
When implementing the present invention, without step 3, directly carry out in step 4 and step 5, remaining parameter and example 3
It is identical, technical process is also identical.
<Comparative example 2>
When implementing the present invention, without step 5, remaining parameter with it is identical in example 3, technical process is also complete
It is exactly the same.
[germination percentage of the Chinese yew seed with same procedure after planting in each example of table 1 and comparative example]
| Germination percentage | |
| Example 1 | 90% |
| Example 2 | 90% |
| Example 3 | 95% |
| Comparative example 1 | 70% |
| Comparative example 2 | 71% |
From upper table 1, it can be seen that due to having carried out step 3 in example 3, the germination percentage of its Chinese yew seed handled shows
Write higher than the situation in comparative example 1 without step 3.Due to having carried out step 5 in example 3, its Chinese yew seed handled
Germination percentage be significantly higher than situation in comparative example 2 without step 5.It can be seen that, the biological heat and ferment of the step three in the present invention
The alternating temperature transformation that sprouting of the element to Chinese yew seed greatly facilitates step 5 in effect, the present invention is stimulated also to Chinese yew kind
Son, which is sprouted, larger facilitation.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With.It can be applied to various suitable the field of the invention completely., can be easily for those skilled in the art
Realize other modification.Therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the example with description.
Claims (1)
1. a kind of germination accelerating method of Chinese yew seed, it is characterised in that comprise the following steps：
Soaked 20-40 seconds Step 1: Chinese yew seed is put into the mercuric chloride solution that mass fraction is 0.1%, it is then clear with water
Wash 3-5 times, then Chinese yew seed mass fraction is soaked 10-15 minutes for 0.1% liquor potassic permanganate, it is then clear with water
Wash 4-6 times；
Step 2: the Chinese yew seed mass fraction that step one is obtained is small for 0.3% Gibberellins solution immersion 24-48
When；
Step 3: the rice bran of 50 parts by weight is mixed with the sawdust of 50 parts by weight, the fermenting agent of 1-2 parts by weight is then mixed into,
Pile bar pile to be fermented, after fermenting 5-7 days, the Chinese yew seed that step 2 is obtained is embedded in bar pile, is taken out after 5-7 days；
Wherein, fermenting agent by bacillus subtilis, brown sugar and water with 1:1-1.5:10-15 weight ratio is mixed；
Step 4: taking vermiculite, soaked, be then charged into a polybag for 0.05% Gibberellins solution with mass fraction, will
The Chinese yew the surface of the seed that step 3 is obtained is also placed in polybag after being polished 3-5 minutes with emery cloth, and is well mixed with vermiculite,
With rope by plastic bag sealing, the polybag was rubbed once every 2-3 hours, rubs 5-10 minutes, is taken after rubbing 5-8 times every time
Go out Chinese yew seed；
Step 5: step 4 is obtained into each Chinese yew seed uses sponge wrapping, and the indoles second for being 80mg/L with concentration
Acid solution soaks sponge, is then placed in pressurized tank, under the conditions of 8MPa, 20 DEG C keep 15-20 minutes, then 5MPa,
Keep 10-15 minutes, finally kept for 10-15 minutes under the conditions of 1MPa, 40 DEG C under the conditions of 30 DEG C；
Before the step one, in addition to：
Chinese yew seed is placed in clear water, after standing 10-20 minutes, the Chinese yew seed floated is removed, taking-up sinks to the bottom
Chinese yew seed, dry；
In the step one, Chinese yew seed is put into the mercuric chloride solution that mass fraction is 1% and soaked 30 seconds, by Chinese yew
Seed mass fraction soaks 12 minutes for 0.1% liquor potassic permanganate；
In the step 2, the weight ratio for the Gibberellins solution that Chinese yew seed is 0.3% with mass fraction is 1:17, immersion
Time is 36 hours；
In the step 3, the cross section of the bar pile is isosceles trapezoid, and the height of the bar pile is 1 meter, by Chinese yew kind
The depth of the 0.4-0.6 rice of son embedment bar pile；
In the step 3, the parts by weight for the fermenting agent being mixed into are 1.2 parts, and fermenting agent is by bacillus subtilis, brown sugar
With water with 1:1.2:12 weight ratio is mixed；
In the step 4, the average grain diameter of vermiculite is 5-8 millimeters, and the weight ratio of vermiculite and Chinese yew seed is 13:1.
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| CN201510454662.0A CN105123018B (en) | 2015-07-30 | 2015-07-30 | The germination accelerating method of Chinese yew seed |
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| CN105123018B true CN105123018B (en) | 2017-07-25 |
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| CN106258080B (en) * | 2016-08-23 | 2019-07-26 | 南阳师范学院 | A method of utilizing biotechnology vernalization Chinese yew seed |
| CN109429614A (en) * | 2018-11-16 | 2019-03-08 | 马步华 | A method of improving Taxus chinensis var. mairei Seeds vigor |
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| US4780987A (en) * | 1983-10-25 | 1988-11-01 | Plant Genetics, Inc. | Method for the preparation of hydrated, pregerminated seeds in gel capsules |
| CN100384317C (en) * | 2005-10-28 | 2008-04-30 | 林敏� | Method for planting southerm yew |
| CN101358176B (en) * | 2007-08-01 | 2011-08-24 | 武汉天惠生物工程有限公司 | Bacillus subtilis, seed-dressing agent and use thereof |
| CN101773031A (en) * | 2009-12-22 | 2010-07-14 | 株洲市神舟红豆杉苗圃 | Chinese yew seedling cultivation technology |
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