CN109258426B - Efficient seedling raising method for stachys sieboldii seeds - Google Patents
Efficient seedling raising method for stachys sieboldii seeds Download PDFInfo
- Publication number
- CN109258426B CN109258426B CN201811173593.6A CN201811173593A CN109258426B CN 109258426 B CN109258426 B CN 109258426B CN 201811173593 A CN201811173593 A CN 201811173593A CN 109258426 B CN109258426 B CN 109258426B
- Authority
- CN
- China
- Prior art keywords
- seeds
- stachys
- culture
- seedling raising
- raising method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a high-efficiency seedling raising method for stachys fimbriata seeds, relates to a seedling raising method for the seeds of a honey plant stachys fimbriata, and aims to solve the technical problems that the area of the stachys fimbriata is greatly reduced, the work of returning back to wet for ploughing is lack of industrial and technical support, and the seedling raising technology of the stachys fimbriata seeds is deficient at present. The seedling raising method comprises the following steps: firstly, collecting ripe seeds of stachys pubescens, and air-drying the seeds at a dry place; secondly, mixing peat soil and vermiculite according to the proportion of 1: 1, fully mixing and preparing to obtain a nutrient medium; thirdly, air-drying the seeds, soaking the seeds in distilled water, and then transferring the seeds into an ethanol solution for soaking; fourthly, soaking the disinfected seeds in warm water at the temperature of 30-60 ℃; fifthly, sowing the seeds into a culture cup; sixthly, placing the culture cup in water for culture; seventhly, managing in the later period. According to the invention, by adopting the temperature-variable germination accelerating method, the seedling emergence time is shortened, the seedling culture success rate is greatly improved, no hormone or pesticide is used in the seedling culture process, the cost is saved, the ecological environment is protected, the cultured seedlings are strong, and the method is suitable for greenhouse large-scale seedling culture.
Description
Technical Field
The invention relates to a seed seedling raising method for a honey source plant stachys fimbriata.
Background
The back-tillage and back-wet engineering can gradually restore the integrity, naturalness and diversity of the wetland ecosystem and improve the fragile ecological environment, and is one of specific measures for restoring the damaged wetland and ensuring that the existing wetland is not degraded any more. However, the natural ecological factors and the current social and economic conditions need to be considered when the cultivation is returned to the wet state, and the reasonable replacement of the birth schedule also influences the smooth implementation of the cultivation-returning and wet-returning policy. At present, China lacks good modes and technologies for returning back to wet and restricts the development of the work of returning back to wet.
Stachys basilica (Stachys basilica) belongs to Stachys of Labiatae, and is originated from wet grassland and river bank, and is produced in Heilongjiang, Jilin, Liaoning, inner Mongolia, etc. In the northeast black bee protection area, the honey yield of the stachys sieboldii is considerable, and once the stachys sieboldii is an important honey source plant in the area (Liu Sheng Jiang, stachys sieboldii, an important honey source plant in the northeast black bee protection area, bee journal, 2017, (2): 31-32.). Through detection, the fringe honey produced in the northeast black bee protection area meets various requirements of national first-grade honey. However, the area of the fringe is greatly reduced due to the influence of climate change and artificial reclamation of wetlands. According to the actual situation of the local, the maohuosu is taken as the main restoration plant in the land-returning wetland, the method has an important promoting effect on the local development of industries mainly based on maohuosu high-end bee products, and can bring comprehensive ecological, economic and social benefits for the land-returning wetland. Seedling transplantation is one of important measures for promoting rapid vegetation recovery and reconstruction, the yield of the stachys sieboldii seeds is large, and the method is suitable for a recovery mode of seedling transplantation, but the technology of efficient seedling culture of the stachys sieboldii seeds is rarely reported.
Disclosure of Invention
The invention provides a method for efficiently breeding stachys fimbriatus seeds, aiming at the technical problems that the area of the stachys fimbriatus is greatly reduced, the work of returning back to wet and returning back to the ploughing state is lack of industrial and technical support and the seedling breeding technology of the stachys fimbriatus seeds is deficient at present, and aims to promote the large-scale development of the stachys fimbriatus in the northeast China.
The method for efficiently breeding the stachys sieboldii seeds is realized according to the following steps:
firstly, seed collection and pretreatment: collecting mature seeds (black brown seeds) of the Teucrium verrucosa for multiple times between the late 7 th and the late 8 th of month according to the phenological period characteristics of the Teucrium verrucosa, removing impurities and shriveled particles, and placing the seeds in a cool and dry place for air drying to obtain air-dried seeds;
secondly, preparing nutrient soil: according to the characteristics of the wild habitat of the stachys pubescens and the requirements on nutrients, mixing peat soil and vermiculite according to the weight ratio of 1: 1, fully mixing and preparing to obtain a nutrient medium;
thirdly, seed disinfection: soaking the air-dried seeds obtained in the step one in distilled water to obtain soaked seeds, then transferring the seeds to an ethanol solution with the volume percentage of 70-75% for soaking for 10-20 s, and washing the seeds clean by sterile water to obtain disinfected seeds;
fourthly, accelerating germination: soaking the disinfected seeds in warm water at the temperature of 30-60 ℃ to obtain seeds subjected to germination accelerating treatment, and preserving in a moistening manner;
fifthly, sowing: filling the nutrient medium in the step two into a culture cup with a vent hole at the bottom, and sowing seeds subjected to germination accelerating treatment in the medium of the culture cup to finish the sowing of the stachys sorghii seeds;
sixthly, regulating and controlling water content: placing the culture cup sown with the stachys sieboldii seeds in water, enabling the water level conditions inside and outside the culture cup to be consistent through the air holes at the bottom, ensuring that the surface of a culture medium in the culture cup is 0-5 cm higher than the water surface, and culturing for 30-45 days to obtain the stachys sieboldii seedlings;
seventhly, post management: removing weeds in the culture cup, thinning out seedlings, and hardening seedlings outdoors to finish the high-efficiency seedling culture of the stachys sieboldii seeds.
The efficient seedling raising method for the stachys fimbriata seeds, disclosed by the invention, has the following beneficial effects:
1. the seed propagation is an important propagation mode of the tassel hirsuta, more mature seeds are obtained by a method of collecting the seeds for multiple times, meanwhile, the damage to the wild tassel hirsuta is avoided, and the method has scientificity and feasibility;
2. by adopting the temperature-variable germination accelerating method, the seedling emergence time is shortened from the original 55 days to 45 days, and the seedling culture success rate is greatly improved;
3. the invention does not use any hormone or pesticide in the whole process, saves cost, is ecological and environment-friendly, and the cultivated seedlings are robust and are suitable for greenhouse large-scale seedling cultivation.
Drawings
FIG. 1 is a histogram of the emergence rate of the seeds of Teucrium lansium of the examples, wherein each group of the immediately adjacent three columns represents 10min, 20min and 120min in order from left to right.
Detailed Description
The first embodiment is as follows: the method for efficiently breeding the stachys sieboldii seeds is implemented according to the following steps:
firstly, seed collection and pretreatment: collecting mature seeds (black brown seeds) of the Teucrium verrucosa for multiple times between the late 7 th and the late 8 th of month according to the phenological period characteristics of the Teucrium verrucosa, removing impurities and shriveled particles, and placing the seeds in a cool and dry place for air drying to obtain air-dried seeds;
secondly, preparing nutrient soil: according to the characteristics of the wild habitat of the stachys pubescens and the requirements on nutrients, mixing peat soil and vermiculite according to the weight ratio of 1: 1, fully mixing and preparing to obtain a nutrient medium;
thirdly, seed disinfection: soaking the air-dried seeds obtained in the step one in distilled water to obtain soaked seeds, then transferring the seeds to an ethanol solution with the volume percentage of 70-75% for soaking for 10-20 s, and washing the seeds clean by sterile water to obtain disinfected seeds;
fourthly, accelerating germination: soaking the disinfected seeds in warm water at the temperature of 30-60 ℃ to obtain seeds subjected to germination accelerating treatment, and preserving in a moistening manner;
fifthly, sowing: filling the nutrient medium in the step two into a culture cup with a vent hole at the bottom, and sowing seeds subjected to germination accelerating treatment in the medium of the culture cup to finish the sowing of the stachys sorghii seeds;
sixthly, regulating and controlling water content: placing the culture cup sown with the stachys sieboldii seeds in water, enabling the water level conditions inside and outside the culture cup to be consistent through the air holes at the bottom, ensuring that the surface of a culture medium in the culture cup is 0-5 cm higher than the water surface, and culturing for 30-45 days to obtain the stachys sieboldii seedlings;
seventhly, post management: removing weeds in the culture cup, thinning out seedlings, and hardening seedlings outdoors to finish the high-efficiency seedling culture of the stachys sieboldii seeds.
In the seventh step of the embodiment, after the stachys fimbriata seedlings grow to about 5cm high, thinning is performed on the culture cups with the number of seedlings more than or equal to 2, and the seedlings with the most vigorous growth are reserved.
The second embodiment is as follows: the difference between the first embodiment and the second embodiment is that each time the stachys fimbriatus seeds are collected in the first step, the inflorescence axis is not damaged, and immature seeds above the inflorescence are kept to be continuously mature.
The third concrete implementation mode: the first step is to collect mature seeds of the tassel hair twice between late 7 th and late 8 th months.
The fourth concrete implementation mode: the difference between this embodiment and the first to third embodiments is that the air-dried seeds in the third step are immersed in distilled water for 1 to 12 hours.
The fifth concrete implementation mode: the fourth difference between the present embodiment and the fourth embodiment is that the air-dried seeds in the third step are immersed in distilled water for 1 to 3 hours.
The sixth specific implementation mode: the difference between the first embodiment and the fifth embodiment is that the seeds sterilized in the fourth step are immersed in warm water at 40-60 ℃ for 10-15 min.
The seventh embodiment: the difference between the first embodiment and the sixth embodiment is that the seeds subjected to the germination accelerating treatment in the fifth step are sowed in the culture cup substrate, and the sowing depth is 0.2-0.8 cm.
The specific implementation mode is eight: the present embodiment is different from the first to seventh embodiments in that 3 to 5 germination-accelerating seeds are sown in each culture cup in the fifth step.
The specific implementation method nine: the difference between the first embodiment and the eighth embodiment is that the surface of the culture medium in the culture cup is 2-4 cm higher than the water surface in the sixth embodiment.
The detailed implementation mode is ten: the difference between the first embodiment and the ninth embodiment is that in the seventh embodiment, thinning is performed after the height of the stachys fimbriatus seedling is 4-6 cm.
Example (b): the method for efficiently breeding the stachys sieboldii seeds is implemented according to the following steps:
firstly, seed collection and pretreatment: according to the characteristic of the phenological period of the Teucrium verrucosa, collecting mature seeds (black brown seeds) of the Teucrium verrucosa twice in the late 7 month and late 8 month, not damaging the inflorescence axis during each collection, keeping the immature seeds above the inflorescence to be continuously mature, removing impurities and shrivelled grains, and placing the seeds in a cool and dry place for air drying to obtain air-dried seeds;
secondly, preparing nutrient soil: according to the characteristics of the wild habitat of the stachys pubescens and the requirements on nutrients, mixing peat soil and vermiculite according to the weight ratio of 1: 1, fully mixing and preparing to obtain a nutrient medium;
thirdly, seed disinfection: soaking the air-dried seeds obtained in the step one in distilled water for 2 hours to obtain soaked seeds, then transferring the seeds into 75% ethanol solution by volume percent to soak for 20 seconds, and washing the seeds clean by sterile water to obtain disinfected seeds;
fourthly, accelerating germination: soaking the disinfected seeds in warm water at 50 ℃ for 10min to obtain seeds subjected to germination accelerating treatment, and preserving in a moist manner;
fifthly, sowing: filling the nutrient medium in the step two into a culture cup with a vent hole at the bottom, wherein the diameter of the culture cup is 80mm, the height of the culture cup is 80mm (the size after spreading), the seeds subjected to germination accelerating treatment are sowed in the medium of the culture cup, the sowing depth is 0.4cm, and 3 seeds are sowed in each culture cup to complete the sowing of the stachys sorghii seeds;
sixthly, regulating and controlling water content: placing the culture cup with the stachys sieboldii seeds sowed in the step five in water, enabling the water level conditions inside and outside the culture cup to be consistent through a vent hole at the bottom, ensuring that the surface of a culture medium in the culture cup is 5cm higher than the water surface, and culturing for 45d to obtain the stachys sieboldii seedlings;
seventhly, post management: removing weeds in the culture cups, thinning the culture cups with the number of seedlings more than or equal to 2 after the stachys fimbriata seedlings grow to be about 5cm high, keeping the seedlings with the most vigorous growth, and hardening the seedlings outdoors.
The effect of the efficient seedling raising of the stachys sieboldii seeds in the embodiment is verified through the following experiments: and setting a test group and a control group for detecting the emergence rate of the stachys sieboldii. Test groups: the test was carried out by changing the temperature of distilled water and the time of germination accelerating treatment in the fourth step of this example. Control group: the difference from the treatment of the test group was that the seeds were placed in distilled water at room temperature (25 ℃ C.) in step four, and the other treatments were the same as those of the test group. The test results are shown in table 1, and a histogram of the emergence rate of the stachys floriduna seeds is plotted by the results in table 1, as shown in fig. 1.
TABLE 1 emergence rate of Stachys tomentosa seeds at different germination temperatures and times
Note: the seedling number of the same pot is more than or equal to 1, the seedling is successfully emerged, and the difference of different lower case letters in the same row reaches the significance level
The emergence rates of the stachys floribundum obtained in the test are shown in table 1 and figure 1, and it can be seen from a graph that the final emergence rate is higher when the seed soaking time is 10min than 20min or 120min when the germination accelerating water temperature is 30-50 ℃. When the germination accelerating water temperature is 60 ℃, the emergence of the stachys sieboldii seeds is obviously inhibited. The germination rate of the control group without warm water seed soaking is 48.8%, and the final germination rate of the stachys sieboldii seeds can be improved by soaking the seeds in warm water at the temperature of 30-50 ℃ for accelerating germination. Therefore, the process effect of soaking the seeds of the stachys sieboldii in warm water at 40/50 ℃ for 10min in the fourth step is the best.
The method provided by the test is simple and easy to implement, and can improve the final emergence rate of the stachys fimbriatus seeds to more than 80%.
Claims (7)
1. The efficient seedling raising method for the stachys sieboldii seeds is characterized by being realized according to the following steps:
firstly, seed collection and pretreatment: collecting mature seeds of the stachys sieboldii for multiple times between 7 and 8 late ten days, removing impurities and shrivelled grains, and placing the seeds in a cool and dry place for air drying to obtain air-dried seeds;
secondly, preparing nutrient soil: mixing peat soil and vermiculite according to the proportion of 1: 1, fully mixing and preparing to obtain a nutrient medium;
thirdly, seed disinfection: soaking the air-dried seeds obtained in the step one in distilled water for 1-3 hours to obtain soaked seeds, then transferring the seeds to an ethanol solution with the volume percentage of 70-75% for soaking for 10-20 s, and washing the seeds clean by sterile water to obtain disinfected seeds;
fourthly, accelerating germination: soaking the disinfected seeds in warm water at 40-60 ℃ for 10-15 min to obtain seeds subjected to germination accelerating treatment, and preserving in a wet manner;
fifthly, sowing: putting the nutrient medium in the step two into a culture cup with a vent hole at the bottom, and sowing seeds subjected to germination accelerating treatment in the medium of the culture cup to finish the sowing of the stachys sieboldii seeds;
sixthly, regulating and controlling water content: placing the culture cup sown with the stachys sieboldii seeds in the step five in water, ensuring that the surface of a culture medium in the culture cup is 0-5 cm higher than the water surface, and culturing for 30-45 days to obtain the stachys sieboldii seedlings;
seventhly, post management: removing weeds in the culture cup, thinning out seedlings, and hardening seedlings outdoors to finish the high-efficiency seedling culture of the stachys sieboldii seeds.
2. The efficient seedling raising method for the eriodictyon sempervirens seeds as claimed in claim 1, wherein each time the eriodictyon sempervirens seeds are collected in the first step, the inflorescence axis is not damaged, and immature seeds above the inflorescence are kept to be mature continuously.
3. The efficient seedling raising method for Mustachys sieboldii seeds according to claim 1, wherein the first step is to collect mature Mustachys sieboldii seeds twice between late 7 to late 8 months.
4. The efficient seedling raising method for the eriodictyon sempervirens seeds as claimed in claim 1, wherein the seeds subjected to the germination accelerating treatment in the fifth step are sowed in a culture cup substrate, and the sowing depth is 0.2-0.8 cm.
5. The efficient seedling raising method for the eriodictyon sempervirens seeds as claimed in claim 1, wherein 3-5 seeds subjected to germination accelerating treatment are sown in each culture cup in the fifth step.
6. The efficient seedling raising method for the eriodictyon sempervirens seeds as claimed in claim 1, wherein the surface of the culture medium in the culture cup is 2-4 cm higher than the water surface in the sixth step.
7. The efficient seedling raising method for the eriodictyon sempervirens seeds as claimed in claim 1, wherein in the seventh step, thinning is carried out after the height of the eriodictyon sempervirens is 4-6 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811173593.6A CN109258426B (en) | 2018-10-09 | 2018-10-09 | Efficient seedling raising method for stachys sieboldii seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811173593.6A CN109258426B (en) | 2018-10-09 | 2018-10-09 | Efficient seedling raising method for stachys sieboldii seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109258426A CN109258426A (en) | 2019-01-25 |
| CN109258426B true CN109258426B (en) | 2020-12-29 |
Family
ID=65195344
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811173593.6A Active CN109258426B (en) | 2018-10-09 | 2018-10-09 | Efficient seedling raising method for stachys sieboldii seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109258426B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113068574A (en) * | 2021-04-02 | 2021-07-06 | 中国科学院东北地理与农业生态研究所 | Method for planting stachys sieboldii |
| CN113597987A (en) * | 2021-09-07 | 2021-11-05 | 黑龙江省德旭农业高科技有限公司 | Cultivation method for artificially domesticated wild stachys narrowa |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173016A (en) * | 2017-06-15 | 2017-09-19 | 刘胜范 | Narrow leaf Herba Stachydis introduce a fine variety artificial purification and rejuvenation implantation methods |
-
2018
- 2018-10-09 CN CN201811173593.6A patent/CN109258426B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN109258426A (en) | 2019-01-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103782913B (en) | A kind of implantation methods of pinellia tuber excised tuber | |
| CN108552056B (en) | Method for rapidly cultivating Baishan ancestor fir seedlings through embryo rescue technology | |
| CN111374048B (en) | Chromosome doubling method of pepper or eggplant haploid plant | |
| CN105432464A (en) | Cultivation method for inducing autotetraploid of paulownia catalpifolia by colchicine | |
| CN104542274A (en) | Culture medium of induced haplobiont for culturing eggplant anther and method of culture medium | |
| CN109258426B (en) | Efficient seedling raising method for stachys sieboldii seeds | |
| CN103340046B (en) | Method for promoting halogeton sativus seeds to rapidly germinate | |
| CN103262741A (en) | Method for prompting growth and development of tobacco floating seedlings | |
| CN106879275B (en) | Method for rapidly and effectively improving germination rate of sophora tonkinensis seeds | |
| CN104957043A (en) | Rapid propagation method of Uncaria sessilifructus Roxb. | |
| CN105941155A (en) | Method for rapidly propagating fraxinus mandshurica by utilizing suspension culture technology | |
| CN105325241A (en) | Method for planting high-quality prunus davidiana rootstock seedlings | |
| CN103703891B (en) | Picriafel-terrae lour seed germination method | |
| CN102257919A (en) | Node cutting propagation technology for cotton | |
| CN105900564B (en) | A kind of method that rare or endangered species beet seeds is promoted efficiently to sprout | |
| CN104641999A (en) | Method for manufacturing celery floating seedling culturing substrate based on mushroom residue | |
| CN107593446A (en) | A kind of fast breeding method of the induction and regeneration of tree peony | |
| CN104160962A (en) | Method for culturing autotetraploid paris polyphylla plant | |
| CN102172218B (en) | Method for restoring activity of inactive lycoris seed | |
| CN104813933B (en) | Method for culturing yam seed tuber by using yam tissue culture seedling | |
| CN106171366A (en) | A kind of hair cuttage rapid propagating method | |
| CN107736210B (en) | High-efficiency breeding method for beautiful millettia root seedlings | |
| CN105145042A (en) | Soil cultivation method for potato virus-free basic seeds | |
| CN106134565A (en) | A kind of chinensis seed is i.e. adopted and is i.e. broadcast the method improving percentage of seedgermination | |
| CN105144912B (en) | A kind of method that largeflower-like honeysuckle flower seed is sprouted |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |