CN108093737B - Method for propagating ricepaperplant pith seedlings and plant cultivation method thereof - Google Patents
Method for propagating ricepaperplant pith seedlings and plant cultivation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of plant cultivation, in particular to a method for propagating ricepaperplant pith seedlings, which comprises the following steps: and (3) carrying out magnetic and electric field composite induction treatment on the seeds of the ricepaperplant pith. Treating the seed of the ricepaperplant pith subjected to the compound induction treatment by the magnetic electric field with plant hormone. Culturing the seed of medulla Tetrapanacis treated by phytohormone on seedbed. Also relates to a method for cultivating the ricepaperplant pith, which carries out seedling propagation by utilizing the ricepaperplant pith seedling propagation method and carries out field planting on the germchit. The method provides a new method for the seedling propagation of the ricepaperplant pith, and has the advantages of low cost, high seedling rate and high field planting survival rate of the obtained seedlings.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for propagating ricepaperplant pith seedlings and a plant cultivation method thereof.
Background
The general name of the dahliang is ricepaperplant pith (Tetrapanax papyriferus) which is a dry stem pith of dahliang plant mandshurica wood and is a conventional and large variety of medicinal materials which are always recorded in the Chinese pharmacopoeia of the past year. According to the record of the traditional Chinese herbal medicine, the dried stem pith (datongcao) of Tongmai has the effects of clearing heat, promoting urination, ventilating and promoting lactation, and is used for treating damp-heat stranguria, edema, oliguria, agalactia and the like. Modern pharmaceutical research finds that the ricepaperplant pith contains effective components such as inositol, uronic acid, polypentaose, arabinose, lactose, galacturonic acid and the like; modern pharmacological research finds that the ricepaperplant pith has certain functions of immunoregulation, oxidation resistance, inflammation diminishing and fever relieving. The medulla Tetrapanacis is widely used in various formulas and Chinese patent medicines for promoting lactation, clearing heat and promoting diuresis, and is the main raw material of Chinese patent medicines such as lactation promoting granules, lactation promoting pills, milk increasing paste, oral liquid for dredging collaterals and promoting lactation, and the like. Has obvious curative effect on urinary system infection, unique effect on dredging channels and collaterals, greatly expands the aspects of treating apoplexy and hemiplegia, is often matched with bone and meat food to cook soup in folk, and has ever-rising market demand.
The sources of the medicinal materials of the ricepaperplant still mainly comprise wild resources, the annual yield is completely influenced by natural factors, and market investigation shows that the ricepaperplant specializers have no large stock, but few stocks of hundreds of kilograms are seen, and most stocks of dozens of kilograms or one or two hundred kilograms are found. The main reasons are that the production area resources are in short supply, the procedures of cutting, peeling, marrow extraction and airing are complicated, and the large goods are scattered and sold without waiting for processing. The production place presents the situation that the processing cannot be sold, and some special dealers even fall into the situation of goods break. Therefore, a propagation technique capable of efficiently propagating the high-quality seedlings of the ricepaperplant pith is lacking at present.
Disclosure of Invention
The first purpose of the invention is to provide a method for propagating the seedlings of the ricepaperplant pith, so as to obtain a new propagation method, effectively propagate the high-quality seedlings of the ricepaperplant pith and have high seedling rate.
The second purpose of the invention is to provide a cultivation method of a ricepaperplant pith plant, which lays a foundation for artificial large-scale planting by quickly breeding ricepaperplant pith high-quality seedlings.
The technical problem to be solved by the invention is realized by adopting the following technical scheme.
The method for propagating the seedlings of the ricepaperplant pith provided by the invention comprises the following steps: carrying out magnetic and electric field composite induction treatment on the seed of the clematis dioica. Treating the seed of the ricepaperplant pith subjected to the compound induction treatment by the magnetic electric field with plant hormone. Culturing the seed of medulla Tetrapanacis treated by phytohormone on seedbed.
The invention also relates to a method for cultivating the ricepaperplant pith, which is used for carrying out seedling propagation and field planting on the ricepaperplant pith.
Pollution and residual harm caused by chemical induction are avoided through magnetic-electric field composite induction and phytohormone treatment, the activity of seed enzyme can be well excited, the seed germination rate and the seed germination vigor are increased, the method provides a new method for seed propagation of the ricepaperplant pith, the cost is low, the seedling rate is high, and the obtained seed field planting survival rate is high.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below. Those whose specific conditions are not specified in the embodiment or examples are carried out according to the conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The method for propagating the germchit of the ricepaperplant and the method for cultivating the plant thereof according to the embodiment of the present invention will be specifically described below.
Some embodiments of the present invention provide a germchit propagation method of ricepaperplant pith, which comprises the following steps:
s1, performing magnetic and electric field composite induction treatment on the seed of the akebia quinata.
The magnetic-electric field composite induction is a process of simultaneously placing seeds in a magnetic field and an electric field to induce the seeds, and the magnetic-electric field composite induction of the ricepaperplant pith seeds can well excite the activity of ricepaperplant pith seed enzymes, increase the germination rate and the germination vigor of the seeds, enhance the disease and insect pest resistance, and make the seedling root system developed and the plants grow strong. The electric field induction also has the effects of disinfecting and sterilizing plant seeds and seedbeds and improving the quality of the plants. The magnetic field induction has small physiological damage to plant seeds and seedlings, the operation is simple and quick, the stress resistance of the seedlings is enhanced, and the water and fertilizer absorption capacity is improved, so that the purpose of increasing yield is achieved.
According to some embodiments, the magnetic field intensity of the seed of the lonicera japonica subjected to the magnetic field composite induction treatment is 800-1200 Gs, preferably 850-1100 Gs, and more preferably 900-1050 Gs; the electric field intensity is 40-60 KV, preferably 45-55 KV, and more preferably 45-50 KV. The magnetic field composite induction is carried out on the seed of the ricepaperplant pith within the range of the magnetic field intensity and the electric field intensity, the characteristics of the ricepaperplant pith seed are suitable, the induction capability of the ricepaperplant pith seed can reach the optimal state, and the vitality of the seed is excited.
According to some embodiments, the treatment time for performing the magnetic electric field composite induction is 5-10 min, so that a good excitation activity effect can be achieved within the time range, and the destructive effect on seeds due to overlong treatment time can be avoided.
S2, treating the ricepaperplant pith seeds subjected to the compound induction treatment by the magnetic electric field with plant hormone.
The growth performance of the seed of the clematis dioica can be positively influenced by the phytohormone, the growth of the leaf and the bud of the clematis dioica can be better stimulated, the growth environment can be better adapted in the propagation process, and the probability and the growth speed of the seedling are improved.
According to some embodiments, the seed of the dahlia speciosa is treated with the phytohormone by soaking the seed of the dahlia speciosa in a solution of the phytohormone in a manner that the seed of the dahlia speciosa can be more fully contacted with the phytohormone, and the phytohormone can uniformly enter the interior of the seed by infiltration to act on the seed.
According to some embodiments, the seed of the medulla Tetrapanacis is soaked for 2-4 hours. It allows the plant hormones to enter the seeds sufficiently without causing damage to the seeds due to the excessively long soaking time.
According to some embodiments, the plant hormone is gibberellin, whose chemical structure belongs to the class of diterpenoid acids, derived from the tetracyclic backbone. The growth of leaves and buds of the seed of the dahliae speciosa can be stimulated by gibberellins. According to some embodiments, the concentration of the gibberellin solution may be 200-400 mL/L. The gibberellin solution within the concentration range can fully act on the seed of the datonia dichotoma, so that the gibberellin in the solution can permeate into the interior of the seed.
S3, culturing the seed of the ricepaperplant pith after being treated by the phytohormone on a seedbed.
Specifically, the seed of the ricepaperplant pith is sowed in the matrix of the seedbed to the seed of the ricepaperplant pith of will broadcasting evenly when broadcasting the seed of the ricepaperplant pith, so that the matrix can fully provide nutrition for the seed of the ricepaperplant pith, improve the seedling rate, and the ricepaperplant pith seed is in the inside of matrix, its surface covers has one deck matrix promptly, makes the matrix can act on the seed comprehensively, in order to satisfy the nutritional demand of its germination and seedling. In addition, supply to take the film arched shed to cultivate on the seedbed, the film arched shed can control the growing environment of datonggao seed better, makes it grow in suitable temperature and environment, reaches higher seedling rate.
According to some embodiments, the thickness of the substrate covered by the seed surface of the Clitocybe maxima is 0.8-1.2 cm, preferably 1 cm. The thickness of the substrate covered by the method can meet the requirements of germination and growth nutrition of the ricepaperplant pith seeds, the ricepaperplant pith seeds have good air permeability, branches and leaves growing after germination can break the soil better, and the temperature and humidity of the ricepaperplant pith seeds can be controlled more favorably.
According to some embodiments, the inoculation membrane is ventilated for 1-2 hours every morning, and water is sprayed every 5-7 days during the seedling raising period. The operation can promote the germination and growth of seeds and meet the requirement on moisture in the seedling raising process.
According to some embodiments, the seedbed comprises a substrate layer, a non-woven fabric, a coarse sand layer and a heatable carbon fiber layer from top to bottom, wherein a probe of a temperature control switch is arranged in the coarse sand layer and is in direct contact with the non-woven fabric.
Through the setting of carbon fiber layer for can heat the matrix on upper portion through it, and then make the process of growing seedlings of datonggao seed go on under the suitable temperature condition, and then improve the seedling rate. The probe through the temperature control switch makes the probe directly contact with the non-woven fabric, so that the heating temperature of the probe can be more accurately controlled, and a better seedling raising effect is achieved.
According to some embodiments, the heating temperature is 23-27 ℃, at which the seed of the clematis dioica can germinate well and grow into seedlings.
According to some embodiments, the matrix layer is a mixture of grass carbon and perlite, the grass carbon can have a good moisturizing effect, so that the datoncao seeds can absorb sufficient moisture, and the perlite has good air permeability, so that the datoncao seeds can ventilate well with the outside. Specifically, the mass ratio of the turf to the perlite can be 8-12: 1, the grass carbon and the perlite are fully mixed according to the proportion, so that the requirements of moisture retention and ventilation can be met simultaneously, and the growth environment of the seed of the datonggao can be better.
According to some embodiments, the thickness of the substrate layer is 12-15 cm. This thickness can make the matrix layer can have fine moisturizing performance, also can satisfy the needs that the root system of growing seedlings of ricepaperplant pith seed grows simultaneously to this thickness can make the heat that produces after the carbon fiber heating of bottom can transmit the matrix near ricepaperplant pith seed well, makes ricepaperplant pith seed can germinate and grow better.
According to some embodiments, the seed bed is disinfected before the seed bed is cultured, and the seed bed can be better prevented from being affected by bacteria by disinfection, so that the probability of seedling is reduced. According to some embodiments, the sterilization mode is to wet the seedbed with a bactericide, so that the seedbed can be sterilized thoroughly by spraying, and meanwhile, the using amount of the bactericide can be saved. The seedbed is preferably wetted by the solution of the carbendazim wettable powder, so that the effect of preventing and treating diseases of various crops caused by fungi (such as adelomycete and polysaccharomyces) is achieved, and the diseases of the rice pith during seedling formation can be reduced. Further, the carbendazim wettable powder is 50% carbendazim wettable powder, and the mass ratio of the carbendazim wettable powder to the solvent is 1: 400-700, the bactericide prepared according to the above mode can kill harmful bacteria in the seedbed, and does not cause adverse effects on the seedling growing process of the seed of the clematis dioica. Of course, in other embodiments, other types of disinfectant solutions can be selected according to actual needs for sterilization and disinfection.
According to some embodiments, the ricepaperplant pith seeds subjected to the magnetic and electric field composite induction treatment are obtained by cleaning, peeling, removing floating seeds and drying in the shade of mature ricepaper pith fruits collected in November. Wherein, the floating seeds after peeling are removed because the floating seeds are shriveled bad seeds, and the integral seedling rate can be improved after the floating seeds are removed.
According to some embodiments, the method for the seed breeding of the ricepaper pith further comprises the following steps: in the whole seedling raising period, the temperature of the seedbed is controlled to be 20-28 ℃, the relative humidity is controlled to be 60-80%, and the germination and growth of the seed of the datongtao grass are facilitated under the conditions of the temperature and the humidity. In some embodiments, when the seedling grows to be more than or equal to 5 percent and a true leaf grows, spraying and applying additional fertilizer by using a humic acid potassium solution and a compound fertilizer aqueous solution, wherein the compound fertilizer refers to a general compound agricultural fertilizer which can be purchased from the market. In the humic acid potassium solution and the compound fertilizer water solution, the mass ratio of the solute to the solvent is 1: 350-450, for example, 400 times of potassium humate solution refers to a mass ratio of potassium humate to water of 1: 400 and mixing the resulting aqueous solution. The concentration can meet the growth requirement of the seedling raising process, and the seedling burning situation caused by overhigh concentration can be avoided. Transplanting the seedlings to a nutrition cup when three true leaves grow out of the seedlings, and topdressing the seedbed every time of transplanting.
Some embodiments of the present invention relate to a method for seedling propagation of ricepaper pith, comprising:
a. collecting mature fruits of medulla Tetrapanacis in November, manually kneading and cleaning, removing pericarp and floating seeds, and drying in the shade.
b. And (3) carrying out magnetic and electric field composite induction treatment on the dried seeds of the ricepaper pith in the shade for 5-10 min, wherein in the process, the magnetic field intensity is 800-1200 Gs, and the electric field intensity is 40-60 KV.
c. And (3) soaking the seeds of the ricepaperplant pith subjected to the compound induction treatment of the magnetic electric field in gibberellin with the concentration of 200-400 mL/L for 2-4 h.
d. Preparing a seedbed: laying a layer of carbon fiber on the lower layer of the seedbed, covering a layer of non-woven fabric to separate the carbon fiber from the matrix, covering a coarse sand layer and the matrix on the non-woven fabric, embedding a probe of a temperature control switch below the coarse sand layer to be in direct contact with the non-woven fabric, wherein the heating temperature is 23-27 ℃, the matrix is turf and perlite, and the proportion is 8-12: 1, the covering thickness of the substrate is 12-15 cm.
e. And (3) seedbed disinfection: and (3) wetting the seedbed by using 400-700 times of 50% carbendazim wettable powder.
f. Seeding in a seedbed: the seed of the ricepaper pith soaked by gibberellin is uniformly sown on a seedbed, a substrate with the thickness of 0.8-1.2 cm is covered on the surface of the seed, the seed is not exposed, a thin film arched shed is arranged on the seedbed, film uncovering and air exchange are carried out for 1-2 hours every morning, and water is sprayed every 5-7 days.
g. Seedling management: controlling the temperature in the greenhouse to be 20-28 ℃ and the relative humidity to be 60-80% in the whole seedling raising period; when the seedling grows to have more than or equal to 5 percent of one true leaf, spraying 350-450 times of potassium humate and 350-450 times of compound fertilizer aqueous solution for topdressing, transplanting the seedling to a nutrition cup when the seedling grows to have three true leaves, and topdressing the seedling bed after each transplanting.
The invention also relates to a method for cultivating the ricepaperplant pith plant, which comprises the following steps of carrying out germchit propagation by using the ricepaperplant pith propagation method and carrying out field planting on the obtained germchit.
Pollution and residual harm caused by chemical induction are avoided through magnetic-electric field composite induction and phytohormone treatment, the activity of seed enzyme can be well excited, the seed germination rate and the seed germination vigor are increased, the method provides a new method for seed propagation of the ricepaperplant pith, the cost is low, the seedling rate is high, and the obtained seed field planting survival rate is high.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
Firstly, mature fruits of the ricepaperplant pith in November are collected, manually rubbed and cleaned, and dried in the shade after removing pericarps and floating seeds.
And secondly, carrying out magnetic and electric field composite induction treatment on the medulla Tetrapanacis seeds dried in the shade for 5min, wherein in the process, the magnetic field intensity is 800Gs, and the electric field intensity is 4 KV.
Then, the seed of the ricepaperplant pith after the compound induction treatment of the magnetic electric field is soaked for 2 hours by gibberellin with the concentration of 200 mL/L.
Then, the seed of the ricepaperplant pith soaked by gibberellin is uniformly sown on a seedbed, a substrate with the thickness of 0.8cm is covered on the surface of the seed, the seed is not exposed, a thin film arched shed is arranged on the seedbed, film uncovering and air exchange are carried out for 1 hour every morning, and water is sprayed every 5 days.
And (5) carrying out field planting on the cultivated seedlings.
Wherein, the seedbed lower floor is laid the one deck carbon fiber and is covered one deck non-woven fabrics again, makes carbon fiber and matrix separate, covers one deck coarse sand layer and matrix again on the non-woven fabrics spins cloth to bury the probe of temperature detect switch below the coarse sand layer with non-woven fabrics direct contact, heating temperature is 23 ℃, the matrix is grass peat and pearlite, the proportion is 8: 1, the thickness of the substrate is 12 cm. And before the seed of the datongcao is cultured, the seedbed is drenched with 400 times of liquid of 50 percent carbendazim wettable powder.
In the whole seedling raising process, the temperature in the greenhouse is controlled to be 20 ℃, and the relative humidity is controlled to be 60%; when the seedling grows to have more than or equal to 5 percent of one true leaf, spraying 350 times of potassium humate and 350 times of compound fertilizer aqueous solution for topdressing, when the seedling grows to have three true leaves, transplanting the seedling to a nutrition cup, and topdressing the seedbed for each time of transplanting.
Example 2
Firstly, mature fruits of the ricepaperplant pith in November are collected, manually rubbed and cleaned, and dried in the shade after removing pericarps and floating seeds.
And secondly, carrying out magnetic and electric field composite induction treatment on the medulla Tetrapanacis seeds dried in the shade for 10min, wherein the magnetic field intensity is 1200Gs and the electric field intensity is 60KV in the process.
Then, the seed of the ricepaperplant pith after the compound induction treatment of the magnetic electric field is soaked for 4 hours by gibberellin with the concentration of 400 mL/L.
Then, the seed of the ricepaperplant pith soaked by gibberellin is uniformly sown on a seedbed, a substrate with the thickness of 1.2cm is covered on the surface of the seed, the seed is not exposed, a thin film arched shed is arranged on the seedbed, film uncovering and air exchange are carried out for 2 hours every morning, and water is sprayed every 7 days.
And (5) carrying out field planting on the cultivated seedlings.
Wherein, the seedbed lower floor is laid the one deck carbon fiber and is covered one deck non-woven fabrics again, makes carbon fiber and matrix separate, covers one deck coarse sand layer and matrix again on the non-woven fabrics spins cloth to bury the probe of temperature detect switch below the coarse sand layer with non-woven fabrics direct contact, heating temperature is 27 ℃, the matrix is grass peat and pearlite, the proportion is 12: 1, the thickness of the substrate is 15 cm. And before the seed of the datongcao is cultured, the seedbed is drenched by 700 times of liquid of 50 percent carbendazim wettable powder.
In the whole seedling raising process, the temperature in the greenhouse is controlled to be 28 ℃, and the relative humidity is 80%; when the seedling grows to have more than or equal to 5 percent of one true leaf, spraying and applying additional fertilizer by using 450 times of potassium humate and 400 times of compound fertilizer aqueous solution, when the seedling grows to have three true leaves, transplanting the seedling to a nutrition cup, and applying additional fertilizer to a seedbed every time of transplanting.
Example 3
Firstly, mature fruits of the ricepaperplant pith in November are collected, manually rubbed and cleaned, and dried in the shade after removing pericarps and floating seeds.
And secondly, carrying out magnetic and electric field composite induction treatment on the medulla Tetrapanacis seeds dried in the shade for 8min, wherein in the process, the magnetic field intensity is 1000Gs, and the electric field intensity is 50 KV.
Then, the seed of the ricepaperplant pith after the compound induction treatment of the magnetic electric field is soaked for 3 hours by gibberellin with the concentration of 300 mL/L.
Then, the seed of the ricepaperplant pith soaked by gibberellin is uniformly sown on a seedbed, a substrate with the thickness of 1cm is covered on the surface of the seed, the seed is not exposed, a thin film arched shed is arched on the seedbed, film uncovering and air exchange are carried out for 1.5 hours every morning, and water is sprayed every 6 days.
And (5) carrying out field planting on the cultivated seedlings.
Wherein, the seedbed lower floor is laid the one deck carbon fiber and is covered one deck non-woven fabrics again, makes carbon fiber and matrix separate, covers one deck coarse sand layer and matrix again on the non-woven fabrics spins cloth to bury the probe of temperature detect switch below the coarse sand layer with non-woven fabrics direct contact, heating temperature is 25 ℃, the matrix is grass peat and pearlite, the proportion is 10: 1, the thickness of the substrate was 13 cm. And before the seed of the datongcao is cultured, the seedbed is drenched by 600 times of liquid of 50 percent carbendazim wettable powder.
In the whole seedling raising process, the temperature in the greenhouse is controlled to be 24 ℃, and the relative humidity is controlled to be 70%; when the seedling grows to have more than or equal to 5 percent of one true leaf, spraying and applying additional fertilizer by 400 times of potassium humate and 400 times of compound fertilizer aqueous solution, when the seedling grows to have three true leaves, transplanting the seedling to a nutrition cup, and applying additional fertilizer to a seedbed every time of transplanting.
Example 4
Firstly, mature fruits of the ricepaperplant pith in November are collected, manually rubbed and cleaned, and dried in the shade after removing pericarps and floating seeds.
And secondly, carrying out magnetic and electric field composite induction treatment on the medulla Tetrapanacis seeds dried in the shade for 6min, wherein in the process, the magnetic field intensity is 900Gs, and the electric field intensity is 45 KV.
Then, the seed of the ricepaperplant pith after the compound induction treatment of the magnetic electric field is soaked for 2.5h by gibberellin with the concentration of 250 mL/L.
Then, the seed of the ricepaperplant pith soaked by gibberellin is uniformly sown on the seedbed, the surface of the seedbed is covered with 0.9 thick matrix, the seed is not exposed, a thin film arched shed is arranged on the seedbed, film uncovering and air exchange are carried out for 1.5 hours every morning, and water is sprayed once every 5.5 days.
And (5) carrying out field planting on the cultivated seedlings.
Wherein, the seedbed lower floor is laid the one deck carbon fiber and is covered one deck non-woven fabrics again, makes carbon fiber and matrix separate, covers one deck coarse sand layer and matrix again on the non-woven fabrics spins cloth to bury the probe of temperature detect switch below the coarse sand layer with non-woven fabrics direct contact, heating temperature is 24 ℃, the matrix is grass peat and pearlite, the proportion is 9: 1, the thickness of the substrate was 13 cm. And before the seed of the datongcao is cultured, the seedbed is drenched by 500 times of liquid of 50 percent carbendazim wettable powder.
In the whole seedling raising process, the temperature in the greenhouse is controlled to be 23 ℃, and the relative humidity is controlled to be 70%; when the seedling grows to have more than or equal to 5 percent of one true leaf, spraying 380 times of potassium humate and 380 times of compound fertilizer aqueous solution for topdressing, when the seedling grows to have three true leaves, transplanting the seedling to a nutrition cup, and topdressing the seedbed for each time of transplanting.
Example 5
Firstly, mature fruits of the ricepaperplant pith in November are collected, manually rubbed and cleaned, and dried in the shade after removing pericarps and floating seeds.
And secondly, carrying out magnetic and electric field composite induction treatment on the medulla Tetrapanacis seeds dried in the shade for 9min, wherein in the process, the magnetic field intensity is 1100Gs, and the electric field intensity is 55 KV.
Then, the seed of the ricepaperplant pith after the compound induction treatment of the magnetic electric field is soaked for 3.5 hours by gibberellin with the concentration of 350 mL/L.
Then, the seed of the ricepaperplant pith soaked by gibberellin is uniformly sown on a seedbed, a substrate with the thickness of 1.1cm is covered on the surface of the seed, the seed is not exposed, a thin film arched shed is arranged on the seedbed, film uncovering and air exchange are carried out for 1.5 hours every morning, and water is sprayed every 6 days.
And (5) carrying out field planting on the cultivated seedlings.
Wherein, the seedbed lower floor is laid the one deck carbon fiber and is covered one deck non-woven fabrics again, makes carbon fiber and matrix separate, covers one deck coarse sand layer and matrix again on the non-woven fabrics spins cloth to bury the probe of temperature detect switch below the coarse sand layer with non-woven fabrics direct contact, heating temperature is 26 ℃, the matrix is grass peat and pearlite, the proportion is 11: 1, the thickness of the substrate was 14 cm. And before the seed of the datongcao is cultured, the seedbed is drenched by 600 times of liquid of 50 percent carbendazim wettable powder.
In the whole seedling raising process, the temperature in the greenhouse is controlled to be 25 ℃, and the relative humidity is controlled to be 75%; when the seedling grows to have more than or equal to 5 percent of one true leaf, spraying 420 times of potassium humate and 420 times of compound fertilizer aqueous solution for topdressing, when the seedling grows to have three true leaves, transplanting the seedling to a nutrition cup, and topdressing the seedbed for each time of transplanting.
The results of calculating the seedling rate of examples 1 to 5 and the survival rate of the obtained seedlings in field planting are shown in table 1:
TABLE 1
| Numbering | Percent seedling rate (%) | Survival rate of field planting (%) |
| Example 1 | 91.3％ | 98.1 |
| Example 2 | 92.4％ | 98.5 |
| Example 3 | 94.7％ | 98.8 |
| Example 4 | 92.8％ | 98.4 |
| Example 5 | 93.5％ | 98.7 |
As can be seen from table 1, the survival rate of the germchits cultured by the method of the embodiment of the present invention can reach more than 90%, and the survival rate of the germchits after field planting can reach more than 98%.
In conclusion, the magnetic and electric field composite induction of the ricepaperplant pith seeds can well excite the activity of ricepaperplant pith seed enzymes, increase the germination rate and the germination vigor of the seeds, enhance the disease and insect resistance, and enable the seedlings to have developed root systems and the plants to grow vigorously. The electric field induction also has the effects of disinfecting and sterilizing plant seeds and seedbeds and improving the quality of the plants. The magnetic field induction has small physiological damage to plant seeds and seedlings, the operation is simple and quick, the stress resistance of the seedlings is enhanced, and the water and fertilizer absorption capacity is improved, so that the purpose of increasing yield is achieved, and the field planting survival rate of the obtained seedlings is high.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (18)
1. A method for reproducing the seedlings of the ricepaperplant pith is characterized by comprising the following steps:
carrying out magnetic field composite induction treatment on the seeds of the akebia quinata, wherein the magnetic field intensity of the seeds of the akebia quinata subjected to the magnetic field composite induction treatment is 850-1100 Gs, the electric field intensity is 45-55 KV, and the treatment time is 5-10 min;
treating the seed of the ricepaperplant pith subjected to the compound induction treatment by a magnetic electric field by using plant hormone, wherein the treatment of the seed of the ricepaperplant pith by using the plant hormone is to soak the seed of the ricepaperplant pith in a solution of the plant hormone; the soaking time is 2-4 hours; the phytohormone is gibberellin, and the concentration of the gibberellin solution is 200-400 mL/L;
culturing the ricepaperplant pith seeds treated by the phytohormone on a seedbed.
2. A method for cultivating a seed of medulla Tetrapanacis as claimed in claim 1, wherein the seed is sown in the matrix of the seedbed and cultured on the seedbed in a plastic film shed.
3. A method for producing seed-seedling of medulla Tetrapanacis as claimed in claim 2, wherein the thickness of the substrate covering the seed surface of medulla Tetrapanacis is 0.8-1.2 cm.
4. The method for propagating ricepaperplant pith seedlings according to claim 2, wherein membrane uncovering and air exchange are carried out for 1-2 hours every morning, and water is sprayed every 5-7 days.
5. The method for propagating ricepaperplant germchit as claimed in claim 1, characterized in that the seedbed comprises from top to bottom a substrate layer, a non-woven fabric, a coarse sand layer and a heatable carbon fiber layer, wherein a probe of a temperature control switch is arranged in the coarse sand layer and is in direct contact with the non-woven fabric.
6. A method for the propagation of ricepaperplant pith seedlings according to claim 5, wherein the heating temperature is 23-27 ℃.
7. The method of claim 5 wherein the matrix layer is a mixture of peat and perlite.
8. A method for seed seedling propagation of ricepaperplant pith as claimed in claim 7, wherein the mass ratio of the grass carbon to the perlite is 8-12: 1.
9. the method for propagating ricepaperplant pith seedlings according to claim 5, wherein the thickness of the matrix layer is 10-15 cm.
10. A method of seed propagation of medulla Tetrapanacis as claimed in claim 1, wherein the seedbed is sterilized before culturing the medulla Tetrapanacis seeds in the seedbed.
11. A method of seed reproduction of ricepaperplant pith as claimed in claim 10, wherein the seedbed is wetted with a bactericide.
12. A method of prometryn seedling reproduction as claimed in claim 11, wherein the seedbed is drenched with a solution of carbendazim wettable powder.
13. A method for seed and seedling propagation of ricepaperplant pith as claimed in claim 12, wherein the wettable carbendazim powder is 50% wettable carbendazim powder, and the mass ratio of the wettable carbendazim powder to the solvent is 1: 400-700.
14. A method for seed propagation of ricepaperplant pith according to any one of claims 1-13, wherein the ricepaperplant pith seeds subjected to magnetic and electric field composite induction treatment are obtained by cleaning, peeling, floating seed removal and drying in the shade of mature ricepaper pith collected in November.
15. A method of seed propagation of ricepaperplant pith as claimed in claim 14, further comprising the step of seed management: in the whole seedling raising period, the temperature of the seedbed is controlled to be 20-28 ℃, and the relative humidity is controlled to be 60-80%.
16. A method for propagating ricepaperplant pith seedlings according to claim 15, characterized in that when the seedlings grow one true leaf more than or equal to 5%, the potassium humate solution and the compound fertilizer aqueous solution are sprayed for topdressing, when the seedlings grow three true leaves, the seedlings are transplanted to a nutrition cup, and each transplantation is carried out for topdressing on a seedbed.
17. The method for propagating ricepaperplant germchit according to claim 16, characterized in that in the potassium humate solution, the mass ratio of potassium humate to the solvent thereof is 1: 350-450, wherein in the compound fertilizer solution, the ratio of the compound fertilizer to the solvent is 1: 350-450.
18. A method for cultivating a plant of medulla Tetrapanacis, comprising performing seedling propagation by the method for seedling propagation of medulla Tetrapanacis of any one of claims 1 to 17, and planting the obtained seedling in a field.
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Denomination of invention: The Method of Seedling Propagation and Plant Cultivation of Datong Grass Effective date of registration: 20230802 Granted publication date: 20210226 Pledgee: Guangxi Beibu Gulf Bank Co.,Ltd. Guilin Branch Pledgor: GUILIN ERASUN MODERN BIO-TECH Inc. Registration number: Y2023450000107 |
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