CN1259821C

CN1259821C - Method for producing fiberflax sterile line and hybrid species

Info

Publication number: CN1259821C
Application number: CN 02153988
Authority: CN
Inventors: 张辉; 陈鸿山; 丁维; 张慧敏
Original assignee: AGRICULTURAL SCIENCES INST OF
Current assignee: AGRICULTURAL SCIENCES INST OF
Priority date: 2002-12-09
Filing date: 2002-12-09
Publication date: 2006-06-21
Anticipated expiration: 2022-12-09
Also published as: CN1418538A

Abstract

The present invention relates to a method for producing a plant species, namely a flax hybrid. Dominant nucleus sterility flax flower blooms sufficiently by nuclear radiation, the fertility keeps stable, and the mark trait is obvious, and thereby, a dual-purpose series material is obtained. The flax hybrid can be finally obtained through the selection of a proportion of a male parent with a high recovery rate and parents of different proportions.

Description

Flax male sterile line and producing method of hybrid

Invention field

The present invention relates to the production method that a kind of plant variety is flax crossbreed, comprise by nuclear radiation making the abundant expansion of spending of dominant genic male sterile flax, it is stable that fertility keeps, and mark property is more obvious, thereby obtain " dual-purpose system " material.Male parent by selecting high recovery rate and this proportioning of father and mother of different proportion finally obtain flax crossbreed.

Background technology

Plants male sterility and heterosis utilization research are the research fields that enjoys attention in China's agricultural science.Entered since the sixties, in flax breeding, the breeding method that the various countries expert pays close attention to the most is the hybrid technology, flax is in having the crop of commodity value, it is one of crop that identifies in the first batch cytoplasmic male sterility, but because the flower of sterile strain can not fully launch, there is not direct value, we have found first strain dominant genic male sterile flax on flax history in 1977, but because a part sterile strain in bud always appears in the offspring of this nuclear sterile material, influenced outcrossing seed-setting rate, thereby made this valuable material fail to be directly used in the production of crossbreed, caused and also do not produce flax crossbreed at present in the world.

Summary of the invention

The objective of the invention is to find a kind of all methods of opening, stable fertility of the sterile flax bud of nuclear that can obtain to make, find the method for producing flax crossbreed simultaneously.

This method is realized by following technical scheme:

(1) adopts the method for nuclear radiation to handle the sterile flax sterile seed of nuclear, make the sterile flax sterile material of nuclear that petal all launches, the petal color is white (can educate strain for blue), obvious, the sterile rate of mark property is stable in its offspring, to have occurred.

Described nuclear radiation is handled, and for example can be treatment with irradiation such as gamma-rays, degree of depth X ray, as using ¹⁸⁷Cs, Co ⁶⁰Gamma-rays shines sterile linseed.

Described nuclear radiation is handled, for example can adopt dosage be 4-20 ten thousand human relations, dose rate be the 50-200 human relations/minute, preferred about 120 human relations/minute gamma-rays (Co for example ⁶⁰Gamma-rays), sterile linseed is shone.

(2) the sterile strain of the open petal that radiation is obtained is carried out brother and sister's friendship with the educated strain that separates, and can obtain that petal is open, the tangible sterile strain female parent of mark property, with the paternal hybrid with strong advantage, can obtain flax crossbreed.

Described brother and sister hand over, and are to produce fertility by the sterile strain after the radiation to separate, and isolate by 1: 1 and can educate strain, can be used as the maintenance line of this male sterile line.The sterile of acquisition is " dual-purpose system ".

Described and paternal hybrid, be meant " dual-purpose system " and male parent by 1: 2: a kind in isolated area, pull out the educated strain (sterile white bud can be educated blue bud) of maternal row squaring period according to mark property, make the sterile strain and the abundant hybridization pollination of male parent row of maternal row, thereby obtain flax crossbreed.

Description of drawings

Fig. 1 is the photo of the sterile flax of nuclear in bud.

Fig. 2 all launches to examine the photo of sterile flax for petal.

Fig. 3 keeps the sterile flax example gene map of nuclear for utilizing brother and sister to hand over.

Fig. 4 is for obtaining " dual-purpose system " sterile material legend.

Fig. 5 is for obtaining flax crossbreed legend.

Embodiment

Introduce example below in conjunction with accompanying drawing.It should be understood that these examples only as an illustration purpose provide, do not limit the scope of the invention.

Example I

Select nuclear sterile flax material (petal can not fully launch) for use, its seed is carried out Co ⁶⁰Gamma-rays is handled, and dosage is 4-20 ten thousand human relations, dose rate be 120 human relations/minute, after seeds treated is sowed, will isolate sterile strain, its petal fully launches, petal is white (can educate strain for blue), mark property is obvious, stigma development is normal.

In isolated area, this sterile material will be isolated by 1: 1 can educate strain with the fully extended sterile material kind of the petal that obtains after the irradiation treatment, carries out keeping the sterility of sterile strain and the opening of flower after brother and sister hand over, and obtains the sterile flax material of available nuclear.Its process as shown in Figure 3.

Example II

Flax sterile material and disease-resistant, more than the high yield improved seeds continuous backcross three generations, can obtain high yield, disease-resistant, sterile rate is stable, sterile flax of nuclear " dual-purpose system " that petal all launches, its process is seen shown in Figure 4.

" dual-purpose system " pressed 1: 2: 1 (1 row male parent with male parent, 2 row are maternal, 1 row male parent) ratio plantation, maternal thickness of sowing is 0.5-3 a times of male parent, the educated strain of pulling out maternal row in squaring period according to mark property (sterile strain is white bud, and can educate strain is blue bud), the fully extended sterile strain of petal can with the male parent hybridization of fully pollinating, because sterile strain stigma development is normal, outcrossing seed-setting rate is very high, so can obtain the crossbreed of strong advantage.Its process as shown in Figure 5.

Claims

1, flax producing method of hybrid is characterized in that:

Adopt dosage be 4-20 ten thousand human relations, dose rate be the 50-300 human relations/minute nuclear radiation handle the sterile flax material of nuclear, the sterile strain that sterile offspring is produced spend all be style of opening and also stable fertility, mark property is more obvious, wherein the sterile strain that the offspring produces in the method be meant the seed of handling after planting, in the whole openings of spending of sterile strain in flowering stage, the color of flower is a white, and stigma development is normal, the outcrossing seed-setting rate height;

Obtain good kernel sterility dual serial and male parent is planted by a certain percentage by continuous backcross, pull out the educated strain that separates in the male sterile line in squaring period, obtain flax crossbreed according to mark property.

2, in accordance with the method for claim 1, it is characterized in that: it is to use Co that the nuclear radiation described in the method is handled ⁶⁰Gamma-rays carries out treatment with irradiation to examining sterile linseed.

3, in accordance with the method for claim 1, wherein said dose rate be 120 human relations/minute.

4, in accordance with the method for claim 1, it is characterized in that: in the method density of kernel sterility dual serial for the 0.5-3 of the normal amount of broadcasting doubly.

5, in accordance with the method for claim 1, it is characterized in that the planting proportion of male parent and kernel sterility dual serial is 1: 2: 1 in the method.