CN1250738C - 应用小麦胚芽无细胞蛋白质合成系统标记蛋白质的一般方法 - Google Patents
应用小麦胚芽无细胞蛋白质合成系统标记蛋白质的一般方法 Download PDFInfo
- Publication number
- CN1250738C CN1250738C CNB018131719A CN01813171A CN1250738C CN 1250738 C CN1250738 C CN 1250738C CN B018131719 A CNB018131719 A CN B018131719A CN 01813171 A CN01813171 A CN 01813171A CN 1250738 C CN1250738 C CN 1250738C
- Authority
- CN
- China
- Prior art keywords
- protein
- selenomethionine
- cell
- protein synthesis
- dhfr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
Abstract
Description
Km(μM) | Vmax(A560nm/μg/min | |
Met-DHFR | 11.2 | 0.143 |
SeMet-DHFR | 10.2 | 0.137 |
Km(μM) | Vmax(A560nm/μg/min) | |
天然型DHFR | 13.2 | 0.127 |
重氢标记DHFR | 14.7 | 0.261 |
Claims (2)
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP220127/2000 | 2000-07-21 | ||
JP220127/00 | 2000-07-21 | ||
JP2000220127 | 2000-07-21 | ||
JP306119/2000 | 2000-10-05 | ||
JP306119/00 | 2000-10-05 | ||
JP2000306119 | 2000-10-05 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1471585A CN1471585A (zh) | 2004-01-28 |
CN1250738C true CN1250738C (zh) | 2006-04-12 |
Family
ID=26596381
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018131719A Expired - Fee Related CN1250738C (zh) | 2000-07-21 | 2001-07-18 | 应用小麦胚芽无细胞蛋白质合成系统标记蛋白质的一般方法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US7074595B2 (zh) |
EP (1) | EP1310564A4 (zh) |
JP (1) | JPWO2002008443A1 (zh) |
CN (1) | CN1250738C (zh) |
AU (2) | AU7274801A (zh) |
CA (1) | CA2416684A1 (zh) |
WO (1) | WO2002008443A1 (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1221481A4 (en) * | 1999-10-15 | 2004-12-08 | Yaeta Dr Endo | MATRIX MOLECULE WITH WIDE APPLICATION AND HIGHLY EFFICIENT, FUNCTIONAL MEANS OF THE CELL-FREE SYNTHESIS OF PROTEINS BY USING THE SAME |
EP1314781A4 (en) * | 2000-08-30 | 2004-11-03 | Endo Yaeta Dr | DESIGN AND CONSTRUCTION OF A TRANSCRIPTION MODEL OF ACELLULAR PROTEIN SYNTHESIS, AND DISCONTINUOUS DILUTION METHOD FOR USE IN SYNTHESIS OF ACELLULAR WHEAT GERM PROTEIN |
CA2477440A1 (en) * | 2002-02-28 | 2003-09-04 | Yaeta Endo | Reaction solution for cell-free protein synthesis, method of preparing the same and protein synthesis method using the same |
WO2004070371A1 (ja) * | 2003-02-10 | 2004-08-19 | Mitsubishi Chemical Corporation | 蛋白質nmr測定方法 |
JP2006211902A (ja) * | 2003-07-29 | 2006-08-17 | Mitsubishi Chemicals Corp | アミノ酸選択的標識化蛋白質合成方法 |
EP1669448A4 (en) * | 2003-09-25 | 2007-10-31 | Cellfree Sciences Co Ltd | PROCESS FOR PRODUCING ANTIGENIC SUBSTANCE |
EP1995324A1 (en) * | 2007-05-22 | 2008-11-26 | ETH Zurich | Cell-free protein synthesis of deuterated proteins for structural and functional studies by NMR spectroscopy |
US20120301904A1 (en) | 2011-04-26 | 2012-11-29 | Prosetta Antiviral, Inc | Multiprotein assemblies |
CN104774801A (zh) * | 2015-04-14 | 2015-07-15 | 云南农业大学 | 一种核酸核糖体结合体系构建方法及应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1983000346A1 (en) * | 1981-07-15 | 1983-02-03 | Roger Kingdon Craig | Human calcitonin precursor polyprotein structural gene |
JP3145431B2 (ja) | 1991-08-20 | 2001-03-12 | 日本酸素株式会社 | 安定同位体標識蛋白質の製造法および試薬キット |
JP3255784B2 (ja) | 1994-01-26 | 2002-02-12 | 弥重太 遠藤 | 蛋白質の合成方法 |
KR0131166B1 (ko) | 1994-05-04 | 1998-04-11 | 최차용 | 무세포시스템에서 단백질을 제조하는 방법 |
JP3683282B2 (ja) * | 1996-10-17 | 2005-08-17 | 三菱化学株式会社 | 遺伝子型と表現型の対応付け分子及びその利用 |
JP3753358B2 (ja) * | 1999-02-24 | 2006-03-08 | 株式会社セルフリーサイエンス | 無細胞タンパク質合成用胚芽抽出液及びその製造方法並びにそれを用いるタンパク質の合成方法 |
AU765632B2 (en) | 1999-05-11 | 2003-09-25 | Cellfree Sciences Co., Ltd. | Preparation containing cell extract for synthesizing cell-free protein and means for synthesizing cell-free protein |
EP1221481A4 (en) * | 1999-10-15 | 2004-12-08 | Yaeta Dr Endo | MATRIX MOLECULE WITH WIDE APPLICATION AND HIGHLY EFFICIENT, FUNCTIONAL MEANS OF THE CELL-FREE SYNTHESIS OF PROTEINS BY USING THE SAME |
-
2001
- 2001-07-18 AU AU7274801A patent/AU7274801A/xx active Pending
- 2001-07-18 EP EP01951912A patent/EP1310564A4/en not_active Withdrawn
- 2001-07-18 CA CA002416684A patent/CA2416684A1/en not_active Abandoned
- 2001-07-18 JP JP2002513925A patent/JPWO2002008443A1/ja active Pending
- 2001-07-18 US US10/333,417 patent/US7074595B2/en not_active Expired - Fee Related
- 2001-07-18 WO PCT/JP2001/006226 patent/WO2002008443A1/ja not_active Application Discontinuation
- 2001-07-18 AU AU2001272748A patent/AU2001272748B2/en not_active Ceased
- 2001-07-18 CN CNB018131719A patent/CN1250738C/zh not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU7274801A (en) | 2002-02-05 |
US20030162245A1 (en) | 2003-08-28 |
US7074595B2 (en) | 2006-07-11 |
CA2416684A1 (en) | 2002-01-31 |
EP1310564A4 (en) | 2004-08-04 |
JPWO2002008443A1 (ja) | 2004-01-08 |
EP1310564A1 (en) | 2003-05-14 |
WO2002008443A1 (fr) | 2002-01-31 |
AU2001272748B2 (en) | 2006-08-31 |
CN1471585A (zh) | 2004-01-28 |
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C10 | Entry into substantive examination | ||
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ASS | Succession or assignment of patent right |
Owner name: WAKENYAKU CO., LTD. CELLFREE SCIENCES CO LTD Free format text: FORMER OWNER: WAKENYAKU CO., LTD. ENDO INDEMNITY JUTA Effective date: 20050225 |
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C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20050225 Address after: Tokyo, Japan, Japan Applicant after: Independent Administrative Corporation Industrial Comprehansive Technologles Institute Co-applicant after: Drug Research Corporation Co-applicant after: Cellfree Sciences Co., Ltd. Address before: Tokyo, Japan, Japan Applicant before: Independent Administrative Corporation Industrial Comprehansive Technologles Institute Co-applicant before: Drug Research Corporation Co-applicant before: Endo Yaeta |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |