CN1215332C - 采用不溶性载体粒子的免疫测定方法、免疫测定试剂及其稳定化方法 - Google Patents
采用不溶性载体粒子的免疫测定方法、免疫测定试剂及其稳定化方法 Download PDFInfo
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- CN1215332C CN1215332C CNB018145825A CN01814582A CN1215332C CN 1215332 C CN1215332 C CN 1215332C CN B018145825 A CNB018145825 A CN B018145825A CN 01814582 A CN01814582 A CN 01814582A CN 1215332 C CN1215332 C CN 1215332C
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- insoluble carrier
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- Expired - Lifetime
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 141
- 239000002245 particle Substances 0.000 title claims abstract description 90
- 238000000034 method Methods 0.000 title claims abstract description 57
- 230000036039 immunity Effects 0.000 title claims abstract description 32
- 239000000872 buffer Substances 0.000 claims abstract description 67
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims abstract description 35
- -1 carbonic acid compound Chemical class 0.000 claims abstract description 35
- 238000006243 chemical reaction Methods 0.000 claims abstract description 35
- 239000012736 aqueous medium Substances 0.000 claims abstract description 20
- 239000006172 buffering agent Substances 0.000 claims description 112
- 238000003018 immunoassay Methods 0.000 claims description 38
- 229920000126 latex Polymers 0.000 claims description 38
- 239000004816 latex Substances 0.000 claims description 37
- 239000000463 material Substances 0.000 claims description 17
- 230000006641 stabilisation Effects 0.000 claims description 16
- 238000011105 stabilization Methods 0.000 claims description 16
- 239000000126 substance Substances 0.000 claims description 12
- 150000001339 alkali metal compounds Chemical class 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims 4
- 150000007513 acids Chemical class 0.000 claims 4
- 229910052799 carbon Inorganic materials 0.000 claims 4
- 229940126657 Compound 17 Drugs 0.000 claims 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract description 4
- 230000007935 neutral effect Effects 0.000 abstract 3
- 230000000087 stabilizing effect Effects 0.000 abstract 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 38
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 34
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 27
- 239000003814 drug Substances 0.000 description 22
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 18
- GAWIXWVDTYZWAW-UHFFFAOYSA-N C[CH]O Chemical group C[CH]O GAWIXWVDTYZWAW-UHFFFAOYSA-N 0.000 description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 17
- 239000004471 Glycine Substances 0.000 description 16
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 16
- 238000002360 preparation method Methods 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 14
- 239000011780 sodium chloride Substances 0.000 description 14
- 235000017557 sodium bicarbonate Nutrition 0.000 description 13
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 13
- 239000013504 Triton X-100 Substances 0.000 description 12
- 229920004890 Triton X-100 Polymers 0.000 description 12
- 239000000725 suspension Substances 0.000 description 12
- 238000013098 chemical test method Methods 0.000 description 11
- 238000004321 preservation Methods 0.000 description 11
- 102000008857 Ferritin Human genes 0.000 description 10
- 108050000784 Ferritin Proteins 0.000 description 10
- 238000008416 Ferritin Methods 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical class OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 7
- 241000283707 Capra Species 0.000 description 6
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 6
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000004793 Polystyrene Substances 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- CCIVGXIOQKPBKL-UHFFFAOYSA-N ethanesulfonic acid Chemical compound CCS(O)(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-N 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 3
- 125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 3
- 230000002421 anti-septic effect Effects 0.000 description 3
- 229940064004 antiseptic throat preparations Drugs 0.000 description 3
- 229960002319 barbital Drugs 0.000 description 3
- 229910021538 borax Inorganic materials 0.000 description 3
- 239000005515 coenzyme Substances 0.000 description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000004328 sodium tetraborate Substances 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 2
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 2
- 108010014663 Glycated Hemoglobin A Proteins 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- 230000004520 agglutination Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 235000013905 glycine and its sodium salt Nutrition 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 229910052809 inorganic oxide Inorganic materials 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 229920000620 organic polymer Polymers 0.000 description 2
- 238000005375 photometry Methods 0.000 description 2
- 239000002861 polymer material Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- KCXFHTAICRTXLI-UHFFFAOYSA-N propane-1-sulfonic acid Chemical compound CCCS(O)(=O)=O KCXFHTAICRTXLI-UHFFFAOYSA-N 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- ZIRURAJAJIQZFG-UHFFFAOYSA-N 1-aminopropane-1-sulfonic acid Chemical compound CCC(N)S(O)(=O)=O ZIRURAJAJIQZFG-UHFFFAOYSA-N 0.000 description 1
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
- MIJDSYMOBYNHOT-UHFFFAOYSA-N 2-(ethylamino)ethanol Chemical compound CCNCCO MIJDSYMOBYNHOT-UHFFFAOYSA-N 0.000 description 1
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 229940058020 2-amino-2-methyl-1-propanol Drugs 0.000 description 1
- LVQFQZZGTZFUNF-UHFFFAOYSA-N 2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazine-1,4-diium-1-yl]propane-1-sulfonate Chemical compound OS(=O)(=O)CC(O)CN1CCN(CC(O)CS(O)(=O)=O)CC1 LVQFQZZGTZFUNF-UHFFFAOYSA-N 0.000 description 1
- IKRZCYCTPYDXML-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;hydrochloride Chemical compound Cl.OC(=O)CC(O)(C(O)=O)CC(O)=O IKRZCYCTPYDXML-UHFFFAOYSA-N 0.000 description 1
- HSXUNHYXJWDLDK-UHFFFAOYSA-N 2-hydroxypropane-1-sulfonic acid Chemical compound CC(O)CS(O)(=O)=O HSXUNHYXJWDLDK-UHFFFAOYSA-N 0.000 description 1
- INEWUCPYEUEQTN-UHFFFAOYSA-N 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CNC1CCCCC1 INEWUCPYEUEQTN-UHFFFAOYSA-N 0.000 description 1
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 description 1
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 description 1
- XCBLFURAFHFFJF-UHFFFAOYSA-N 3-[bis(2-hydroxyethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCCN(CCO)CC(O)CS(O)(=O)=O XCBLFURAFHFFJF-UHFFFAOYSA-N 0.000 description 1
- 239000007991 ACES buffer Substances 0.000 description 1
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 239000008000 CHES buffer Substances 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 description 1
- GIZQLVPDAOBAFN-UHFFFAOYSA-N HEPPSO Chemical compound OCCN1CCN(CC(O)CS(O)(=O)=O)CC1 GIZQLVPDAOBAFN-UHFFFAOYSA-N 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- MKWKNSIESPFAQN-UHFFFAOYSA-N N-cyclohexyl-2-aminoethanesulfonic acid Chemical compound OS(=O)(=O)CCNC1CCCCC1 MKWKNSIESPFAQN-UHFFFAOYSA-N 0.000 description 1
- 239000007990 PIPES buffer Substances 0.000 description 1
- 108010077895 Sarcosine Proteins 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical group C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 1
- 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 description 1
- YVZLYNHKJASIHA-UHFFFAOYSA-L [Na+].[K+].OP(O)([O-])=O.OP(O)([O-])=O Chemical compound [Na+].[K+].OP(O)([O-])=O.OP(O)([O-])=O YVZLYNHKJASIHA-UHFFFAOYSA-L 0.000 description 1
- PICKAGXPNZYMPX-UHFFFAOYSA-N [Na].Cl.CN(C)CC(O)=O Chemical compound [Na].Cl.CN(C)CC(O)=O PICKAGXPNZYMPX-UHFFFAOYSA-N 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- CBTVGIZVANVGBH-UHFFFAOYSA-N aminomethyl propanol Chemical compound CC(C)(N)CO CBTVGIZVANVGBH-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- ZRBROGSAUIUIJE-UHFFFAOYSA-N azanium;azane;chloride Chemical compound N.[NH4+].[Cl-] ZRBROGSAUIUIJE-UHFFFAOYSA-N 0.000 description 1
- 229960000796 barbital sodium Drugs 0.000 description 1
- AENYAMPVQFAKHY-UHFFFAOYSA-N boric acid;potassium Chemical compound [K].OB(O)O AENYAMPVQFAKHY-UHFFFAOYSA-N 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 description 1
- 229910000020 calcium bicarbonate Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- HXOLFXRMWWHLMH-UHFFFAOYSA-L disodium boric acid carbonate Chemical compound [Na+].[Na+].OB(O)O.[O-]C([O-])=O HXOLFXRMWWHLMH-UHFFFAOYSA-L 0.000 description 1
- QRYVOMJIXGQPMF-UHFFFAOYSA-L disodium diacetate hydrochloride Chemical compound [Na+].[Na+].Cl.CC([O-])=O.CC([O-])=O QRYVOMJIXGQPMF-UHFFFAOYSA-L 0.000 description 1
- HDFXRQJQZBPDLF-UHFFFAOYSA-L disodium hydrogen carbonate Chemical class [Na+].[Na+].OC([O-])=O.OC([O-])=O HDFXRQJQZBPDLF-UHFFFAOYSA-L 0.000 description 1
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 description 1
- CSVGEMRSDNSWRF-UHFFFAOYSA-L disodium;dihydrogen phosphate Chemical compound [Na+].[Na+].OP(O)([O-])=O.OP(O)([O-])=O CSVGEMRSDNSWRF-UHFFFAOYSA-L 0.000 description 1
- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hcl hcl Chemical compound Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 229910000765 intermetallic Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- QWDJLDTYWNBUKE-UHFFFAOYSA-L magnesium bicarbonate Chemical compound [Mg+2].OC([O-])=O.OC([O-])=O QWDJLDTYWNBUKE-UHFFFAOYSA-L 0.000 description 1
- 229910000022 magnesium bicarbonate Inorganic materials 0.000 description 1
- 235000014824 magnesium bicarbonate Nutrition 0.000 description 1
- 239000002370 magnesium bicarbonate Substances 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
试剂开封后的天数(天) | 实施例1 | 实施例2 | 实施例3 | 比较例1 |
0 | 61 | 62 | 62 | 62 |
14 | 66 | 62 | 60 | 74 |
28 | 69 | 62 | 57 | 83 |
R1试剂开封后天数(天) | 实施例4 | 实施例5 | 比较例2 |
0 | 6.0 | 6.1 | 6.0 |
14 | 6.0 | 6.0 | 6.4 |
28 | 6.1 | 5.8 | 6.8 |
R1试剂开封后天数(天) | 实施例6 | 实施例7 | 比较例3 |
0 | 6.0 | 6.1 | 6.1 |
14 | 6.4 | 6.1 | 6.8 |
28 | 6.5 | 6.1 | 7.5 |
Claims (21)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP226805/2000 | 2000-07-27 | ||
JP2000226805 | 2000-07-27 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1449494A CN1449494A (zh) | 2003-10-15 |
CN1215332C true CN1215332C (zh) | 2005-08-17 |
Family
ID=18720354
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018145825A Expired - Lifetime CN1215332C (zh) | 2000-07-27 | 2001-07-27 | 采用不溶性载体粒子的免疫测定方法、免疫测定试剂及其稳定化方法 |
Country Status (8)
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US (2) | US20040053426A1 (zh) |
EP (1) | EP1304571A4 (zh) |
JP (1) | JP4950406B2 (zh) |
KR (1) | KR20030022314A (zh) |
CN (1) | CN1215332C (zh) |
AU (1) | AU2002227509A1 (zh) |
CA (1) | CA2417128A1 (zh) |
WO (1) | WO2002010760A1 (zh) |
Families Citing this family (7)
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US7324861B1 (en) * | 2006-11-30 | 2008-01-29 | Chun Bin Zheng | Numerical control system and method for laser cutting SMT stencil |
JP4874827B2 (ja) * | 2007-02-02 | 2012-02-15 | ベックマン コールター, インコーポレイテッド | 分析装置 |
CN101796420B (zh) | 2007-10-04 | 2013-09-11 | 松下电器产业株式会社 | 分析用仪器和使用该分析用仪器的分析装置及分析方法 |
JP6168497B2 (ja) * | 2012-03-30 | 2017-07-26 | 国立大学法人 岡山大学 | 抗体検出用試薬の製造方法、及びその用途 |
CN104237522B (zh) * | 2012-12-03 | 2016-12-21 | 武汉生之源生物科技股份有限公司 | 脂联素含量检测试剂盒及其制备方法 |
CN106198415A (zh) * | 2016-07-12 | 2016-12-07 | 安徽伊普诺康生物技术股份有限公司 | 一种测定糖化血红蛋白的试剂盒及其制备方法 |
WO2020167411A1 (en) | 2019-02-15 | 2020-08-20 | Siemens Healthcare Diagnostics Inc. | Calibrators and controls for the determination of percent glycated hemoglobin in a patient's liquid test sample |
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US4367279A (en) * | 1974-09-06 | 1983-01-04 | Eastman Kodak Company | Silver halide complexing agents of sulfones, nitriles, and onium salts |
JPS5587047A (en) * | 1978-12-25 | 1980-07-01 | Teikoku Hormone Mfg Co Ltd | Immunological measurement of hapten |
US4331862A (en) * | 1979-02-23 | 1982-05-25 | Ryan Wayne L | Method for calibrating a particle counting machine and a calibration standard therefor |
US4395524A (en) * | 1981-04-10 | 1983-07-26 | Rohm And Haas Company | Acrylamide copolymer thickener for aqueous systems |
US4847209A (en) * | 1987-11-09 | 1989-07-11 | Miles Inc. | Latex agglutination immunoassay in the presence of hemoglobin |
US5162237A (en) * | 1988-04-11 | 1992-11-10 | Miles Inc. | Reaction cassette for preforming sequential analytical assays by noncentrifugal and noncapillary manipulations |
CA2061371A1 (en) * | 1991-03-15 | 1992-09-16 | Edna Antonian | Agglutination immunoassay |
US5470759A (en) * | 1992-06-10 | 1995-11-28 | Fujirebio, Inc. | Anti-glycated hemoglobin monoclonal antibody and method for measuring glycated hemoglobin |
JP2596321B2 (ja) * | 1992-06-16 | 1997-04-02 | 富士レビオ株式会社 | 糖化ヘモグロビンの測定方法 |
JPH06167495A (ja) * | 1992-07-14 | 1994-06-14 | S R L:Kk | 凝集イムノアッセイ法 |
TW378213B (en) * | 1992-11-04 | 2000-01-01 | Shionogy Seiyaku Kk | Basophil-binding monoclonal antibody, method for separation of basophils, method for chemical mediator released from basophils, and testing method for release of basophil-derived chemical mediators |
US5491071A (en) * | 1993-08-03 | 1996-02-13 | Abbott Laboratories | Reagents and methods for the detection and quantification of testosterone in fluid samples |
JPH0862218A (ja) * | 1994-08-25 | 1996-03-08 | S R L:Kk | リウマチ因子の測定方法 |
JPH08193999A (ja) * | 1995-01-13 | 1996-07-30 | Sekisui Chem Co Ltd | 免疫測定法 |
JPH1010127A (ja) * | 1996-06-25 | 1998-01-16 | Sekisui Chem Co Ltd | 凝集イムノアッセイ法 |
US5858648A (en) * | 1996-11-04 | 1999-01-12 | Sienna Biotech, Inc. | Assays using reference microparticles |
US5981296A (en) * | 1997-02-18 | 1999-11-09 | Dade Behring Inc. | Stabilization of particle reagents |
EP0983509B1 (en) * | 1997-05-14 | 2005-08-17 | Biosite Diagnostics Inc. | Rapid evaluation of the ratio of biological molecules |
US6174728B1 (en) * | 1998-04-03 | 2001-01-16 | Avl Medical Instruments Ag | Control or calibration standard for use with instruments for optical measurement of hemoglobin concentration in blood samples |
DE19857897A1 (de) * | 1998-12-15 | 2000-06-21 | Basf Ag | Verfahren zur Herstellung wäßriger Polymerdispersionen |
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2001
- 2001-07-27 EP EP01984389A patent/EP1304571A4/en not_active Withdrawn
- 2001-07-27 US US10/333,785 patent/US20040053426A1/en not_active Abandoned
- 2001-07-27 WO PCT/JP2001/006500 patent/WO2002010760A1/ja active Application Filing
- 2001-07-27 KR KR10-2003-7001106A patent/KR20030022314A/ko not_active Application Discontinuation
- 2001-07-27 JP JP2002516636A patent/JP4950406B2/ja not_active Expired - Lifetime
- 2001-07-27 CN CNB018145825A patent/CN1215332C/zh not_active Expired - Lifetime
- 2001-07-27 CA CA002417128A patent/CA2417128A1/en not_active Abandoned
- 2001-07-27 AU AU2002227509A patent/AU2002227509A1/en not_active Abandoned
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Also Published As
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KR20030022314A (ko) | 2003-03-15 |
CA2417128A1 (en) | 2003-01-24 |
US8431415B2 (en) | 2013-04-30 |
EP1304571A4 (en) | 2007-06-27 |
WO2002010760A1 (fr) | 2002-02-07 |
CN1449494A (zh) | 2003-10-15 |
US20040053426A1 (en) | 2004-03-18 |
AU2002227509A1 (en) | 2002-02-13 |
EP1304571A1 (en) | 2003-04-23 |
JP4950406B2 (ja) | 2012-06-13 |
US20090286330A1 (en) | 2009-11-19 |
JPWO2002010760A1 (ja) | 2004-01-15 |
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