CN1212112C - Skin care cosmetics containing recombinant human auxin - Google Patents
Skin care cosmetics containing recombinant human auxin Download PDFInfo
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- CN1212112C CN1212112C CN 03129040 CN03129040A CN1212112C CN 1212112 C CN1212112 C CN 1212112C CN 03129040 CN03129040 CN 03129040 CN 03129040 A CN03129040 A CN 03129040A CN 1212112 C CN1212112 C CN 1212112C
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- skin
- cosmetics
- recombinant human
- human somatropin
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- 229930192334 Auxin Natural products 0.000 title abstract 4
- 239000002363 auxin Substances 0.000 title abstract 4
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- 229960004532 somatropin Drugs 0.000 claims description 39
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
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Abstract
The present invention relates to a cosmetic containing recombinant human auxin for nursing skin, which is prepared by adding the recombinant human auxin of genetic engineering product into ordinary cosmetic sold in the market in the ratio of 1: (1000 to 100000). The cosmetic containing recombinant human auxin of the present invention is smeared on the skin of an old rat, and the experiment result shows that the cosmetic not only can activate the growth, the differentiation and the proliferation of epidermal cells, but also can increase and coarsen dermis intradermal collagenous fibers and elastic fibers, increase blood supply, improve skin microcirculation and promote the restoration and the proliferation of cells; therefore, the skin cells obtain sufficient nutrient and grow vigorously, the cell activity is enhanced, wrinkle of skin is prevented and eliminated, so the skin becomes smooth, fine and tender and has elasticity. The actual effect of the present invention used on the human body skin corresponds to the experimental result on the rat, which indicates that the cosmetic has the functions of nursing skin, reversing aging and resisting aging; the present invention is a new generational cosmetic containing genetic engineering products and has good development and market prospects.
Description
Technical field
The present invention relates to contain the cosmetics of recombinant product, specifically relate to the cosmetics of a kind of recombinant human somatropin of containing (rhGH, recombinant human Growth Hormone) nursing skin.
Background technology
As everyone knows, the human lives is subjected to inevitably from every aspect (society, environment, nutrition, disease etc.) influence in the complicated social environment, will play irreversible effect to people's health, progressively old and feeble, this is the mathematical fact.The skin of mammal (comprising the mankind) is made up of epidermis and corium.Epidermis mainly is made up of basal layer cell, granular cell and horny layer; Corium is made up of connective tissue, mainly contain abundant collagen fiber and elastic fiber and hair follicle and sebaceous gland etc. (chief editor such as Li Fuxiang, " histology's diagram ", nineteen ninety, P.287).
Yet, be accompanied by age growth, go through the hardships years for many years, very big variation has taken place in the cellularity of skin and function, and horny layer of epidermis thickens, and basal layer cell quantity reduces, and cell viability weakens; Equally, intradermal collagen fiber and elastic fiber quantity reduce, attenuate, the miopragia of their secretion collagen protein and elastin laminin.Originally their three-dimensional lattice of composition is supporting skin, makes skin plentiful, high resilience.Owing to old and feeble reason, its organizational structure neat, commissure mutually is destroyed, and the lines of skin has occurred, especially loose, sagging, the attenuation of facial skin, subcutaneous fat and loss of moist, and skin follows the string, and presents old Signs.
In the face of the aging phenomenon of skin, whether people feel simply helpless, delay, stop, reverse the aging of skin? this is the scientists question of common concern.Since last century end, " gene " cosmetics, be subjected to the favor of consumer, as if the trend that replaces traditional cosmetics and chemical toilet arranged, yet, so-called " gene " cosmetics, only refer to utilize the bioactie agent of technique for gene engineering development, reach the purpose of maintenance skin, as human fiber's trophic factors (aFGF, bFGF), human keratinocyte growth factor (KGF-2) etc.Chinese patent CN 1088088 disclosed " a kind of cosmetics that contain skin growth hormone ", described skin growth hormone is to extract from the fresh embryo skin of mammal, its main component is protein, polypeptide and polysaccharide and glycoprotein etc., still contains trace element in addition.This skin growth hormone also is a kind of bioactie agent in fact.In addition, be reported in addition and add the natural active protein component in the cosmetics, for example amount is the mixture of natural active protein of the artificial extraction more than at least two kinds of 15-20% in " cellular-restoring beauty cream " of CN 1037455 report, as human growth hormone etc., only be indicated as " the artificial natural active protein that extracts; and think that this reactive protein is that different activities protein more than at least two kinds mixes; " in the literary composition, but do not write the source and the performance of described human growth hormone in the literary composition clearly as human growth hormone etc.As everyone knows, the human growth hormone is if adopt the artificial method of extracting to obtain from people's hypophysis, and it is extremely difficult to originate.Undoubtedly, " skin growth hormone " of indication and " human growth hormone " in the above-mentioned binomial publication, the biological extraction mixture of only be that one kind of multiple components, composition are indeterminate, molecular structure and molecular weight being entirely ignorant of.
As far back as U.S. research on anti-senescence pioneer doctor Rudman.D in 1985, going up for the first time at " U.S.'s aged society magazine ", the raiser growth hormone can reverse old and feeble viewpoint, and test through clinical comparison, after the old people end user growth hormone in 60-80 year is provided, the physiological age youth clinical evidence in 10-20 year.(Rudman.D.，et?al，Journal?of?theAmerican?Geriatrics?Society，33(1985)：800-7)。
1998, U.S. Ronald doctor Klatz (president of U.S. research on anti-senescence institute), spell out in his monograph " Growth Young With hGH " book: the human growth hormone is that first kind in the world (also be so far unique a kind of) can reverse the material of old and feeble (just can make us getting back to health status at an early age), it can delay, stop, reverse old and feeble (Ronald Klatz et al., " Growth Young With hGH ", New York, Harper Perennial, 1998).
For this reason, the present invention utilizes the recombinant human somatropin, and effect is studied to senile skin, thereby develops and developed nursing of new generation, delay and reversed the cosmetics that contain the recombinant product recombinant human somatropin of skin aging.
Summary of the invention
The invention provides a kind of cosmetics of the recombinant human somatropin's of containing nursing skin, it has nursing skin, reverses old and feeble and antidotal function.
The recombinant human somatropin of containing one thousandth to ten ten thousand/part by weight amount in the cosmetics of nursing skin of the present invention.The cosmetics that the present invention contains the recombinant human somatropin be by the recombinant human somatropin with one thousandth to ten ten thousand/ the amount of part by weight mix in the component of commercial goods cosmetics and make.
Described recombinant human somatropin is the recombinant product that utilizes technique for gene engineering to produce, it is identical with the structure and the physiological function of the natural human growth hormone that is obtained by extraction in people's hypophysis cerebri, the protein that it is made up of 191 aminoacid, molecular weight are 22000 dalton.A large amount of both at home and abroad research datas prove, this protein has can control cell, skeleton, muscle is grown up and different physiological roles such as activate cell functions effectively.
Commercial goods cosmetics commonly used, its component generally comprises emulsifying agent, emollient, wetting agent, thickening agent, surfactant, antioxidant, nertralizer, antiseptic, pearling agent is transferred the pH agent, essence, water etc.Certainly, component is allowed a choice and adjusts along with the specific (special) requirements of concrete cosmetics kind.
The cosmetics that the present invention contains the recombinant human somatropin comprise: protective skin cream, milky lotion, bath gel, eye cream, surfactant, cleansing milk, nourishing pack, massage cream (frost), anti-wrinkle cream, hand cream and weight reducing cosmetics etc.
The present invention contains the preparation method of recombinant human somatropin's cosmetics, its routine operation is at first by the requirement of cosmetic product, its substrate component is weighed in proportion, stir down at 70-80 ℃, make it become the emulsifying shape, be cooled to 40 ℃ then, add antiseptic again, essence and with the recombinant human somatropin of one thousandth to ten ten thousand/part by weight, stir.
The cosmetic applications histology of nursing skin of the present invention and SABC method, senile rat is tested, observe the change that the cytology is taken place before and after the rat skin coating cosmetics of the present invention, with the effect of differentiating cosmetics of the present invention (see embodiment for details, protect to hold up frost be example) with coating.
At first observe senile rat skin applies 4 weeks of commercial goods protective skin cream substrate (not containing rhGH) (matched group (II)) every day, result of the test is: Skin Cell is learned to observe and is found, no matter be epidermis inner cell structure, or intradermal fibroblast, collagen fiber, elastic fiber, erythrocyte number, basic the same with matched group I (skin is without any processing), no change.Test shows, the commercially available cosmetic base of the simple coating of senile rat skin does not have any effect (seeing Fig. 1,3,5,7) to the nursing and the defying age of skin.
And the experimental result of experimental group senile rat skin coating protective skin cream of the present invention (adding the recombinant human somatropin in the commercially available protective skin cream substrate) shows, following significant change takes place skin texture:
1, the variation of skin surface cell
Be not difficult to find that the skin surface of old rats is very thin from Fig. 1, cuticle thickening, the cell level reduces, and basal layer cell presents " atrophy " state, and cell viability weakens, and spine cell and granular cell can't distinguish, Here it is typical geroderma.
Yet, 10 senile rats of experimental group are coated to contain (see figure 2) behind recombinant human somatropin's protective skin cream, nearly all mouse skin shows that the human growth hormone is by with after the surface of cell membrane growth hormone receptor combines, growth, differentiation, the propagation of epidermis cell have been activated, showing as the cell level significantly increases, epidermis thickens, especially basal layer cell is column, and with epidermis in vertical state, brought into play the propagation function of nutrient substance, recover the cell homergy, keep softness, delicacy, the high resilience of skin.
2, the variation of intradermal collagen fiber and elastic fiber
Corium mainly is made of compact connective, and wherein collagen fiber, elastic fiber and connective tissue occupy main component.Along with the growth in age (Mus age), collagen fiber and elastic fiber attenuate, the miopragia of their secretory protein (seeing Fig. 3 and Fig. 5).
Yet after experimental group senile rat skin contained recombinant human somatropin's protective skin cream in coating, collagen fiber and elastic fiber not only quantity increased, and fiber increases slightly, caused senile skin renew one's youth vigor and high resilience (seeing Fig. 4 and Fig. 6).
3, the variation of intradermal blood vessel
Histological observation exists abundant vasoganglion and lymph net in the corium, they provide the important channel of skin-nourishing.The aging of skin and endovascular nutrition supply have direct relation.
As if not only quantity is few can to find in the senile rat skin blood vessel from Fig. 7 matched group, and the blood flow supply is not enough, the Skin Cell source of nutrition is affected.Thereby, cause skin attenuation to occur, loose, the symptoms such as forfeiture of subcutaneous fat and moisture.The later (see figure 8) of protective skin cream that contains the recombinant human somatropin when the coating of experimental group senile rat skin, situation is greatly taken on a new look, and blood capillary quantity increases, and blood vessel significantly increases slightly, hemocyte increases, the vessel inner blood supply increases, and has improved skin microcirculation, and senile skin obtains abundant nutrition again, epidermal layer cells is bred rapidly, metabolism is vigorous, and the variation of amount and matter has taken place for intradermal collagen fiber and elastic fiber, thereby makes senile skin recover the youth.
The present invention contains the cosmetics of recombinant human somatropin's nursing skin after using on the human body skin, is subjected to vast the young and the middle aged and old friend's welcome deeply, and generally reflection is good; A middle-aged women, originally her skin of face was not only black but also thick, behind the use fortnight, found that pleasantly surprisedly it is white and delicate that skin becomes gradually; The another one elderly woman uses the wrinkle of rear face to reduce, and skin brightens, high resilience; Also have an old man, coating back pachylosis has disappeared, and presents smooth and flexible; People with after generally reflect instant effect, whiteness of skin, delicacy, ruddy.
In a word, the present invention contains the cosmetics of recombinant human somatropin's nursing skin, matches in actual effect on the human body skin and the experimental result on rat skin, has played nursing skin really and has reversed aging and function in delaying senility.
Advantage of the present invention: the cosmetics that the present invention contains recombinant human somatropin's nursing skin are cosmetics that a new generation contains recombinant product.The recombinant human somatropin that the present invention uses, not only its molecular structure and molecular weight are clear, and physiological function is clear and definite, and is the highly purified recombinant product of single component.The recombinant human somatropin is mixed in the cosmetics component of commercial goods and can make, and preparation method is easy, and cost is low, and the cosmetics result of use is splendid.Senile rat skin coating cosmetics experimental result of the present invention shows that the cosmetics that add the recombinant human somatropin can promote the vessel inner blood supply to increase, and improve skin microcirculation, strengthening skin-nourishing supplies with, make cell metabolism vigorous, the change colour of skin, gloss is ruddy; The recombinant human somatropin can combine with the cell surface specific receptor, stromal cell multiple fission in the regulation and control epidermis, and Growth and Differentiation, metabolism is vigorous, promotes protein synthesis, has delaying decrepitude of skin, functions such as repair cell; Cosmetics with recombinant human somatropin can promote intradermal fibroblast synthetic and secretion collagen protein and elastic fiber albumen, strengthen skin elasticity, stop and the elimination wrinkle of skin, make the skin smooth delicacy.The present invention's cosmetics of containing the recombinant human somatropin have and reverse old and feeble, nursing skin and antidotal function in a word, are subjected to the favor of consumer deeply.
Description of drawings
Fig. 1 is matched group senile rat skin texture (20 * 5, HE dyeing).
Fig. 2 is the skin texture (20 * 5, HE dyeing) after the coating of experimental group senile rat contains recombinant human somatropin's cosmetics.
Fig. 3 is matched group senile rat skin texture (20 * 5, dual staining),
Collagen fiber take on a red color, and elastic fiber is blue color.
Fig. 4 is the skin texture (20 * 5, dual staining) after the coating of experimental group senile rat contains recombinant human somatropin's cosmetics,
Collagen fiber take on a red color, and elastic fiber is blue color.
Fig. 5 is matched group senile rat skin texture (20 * 5, dual staining),
Elastic fiber is blue color.
Fig. 6 is the skin texture (20 * 5, dual staining) after the coating of experimental group senile rat contains recombinant human somatropin's cosmetics,
Elastic fiber is blue color.
Fig. 7 is matched group senile rat skin texture (4 * 5, the SABC method),
It is brown that erythrocyte is.
Fig. 8 is the skin texture (4 * 5, the SABC method) after the coating of experimental group senile rat contains recombinant human somatropin's cosmetics,
It is brown that erythrocyte is.
The specific embodiment
The preparation of embodiment 1 massage cream
Set of dispense ratio: 3.5%, No. 26 white oil 9% of glyceryl monostearate 6.5%, ten six octadecanol, sad triglyceride 4%, isopropyl palmitate 10%, dimethicone 2%, methyl hydroxybenzoate 0.2%, propylparaben 0.1%, triethanol ammonium 10%, sodium sulfite 0.1%, essence 0.1%, carbopol 940 5%, (rhGH is provided by Shanghai Sai Er biotech company rhGH0.1%, New Drug Certificate numbering (98) medicine authorized No. S-11 number), deionized water is added to 100%.Above-mentioned substrate component is heated to 70-80 ℃, after fully mixing, carries out emulsifying, treat to add essence and rhGH when temperature drops to 45 ℃ of left and right sides, stir evenly, promptly get massage cream.
The preparation of embodiment 2 protective skin cream
Set of dispense ratio: monoglyceride 3%, sad triglyceride 2%, synthetic squalane 5%, dimethicone 1%, 16 octadecanol 3%, methyl hydroxybenzoate 0.2%, propylparaben 0.1%, glycerol 5%, isoparaffin/laureth-7 1%, essence 0.1%, rhGh0.01%, deionized water is added to 100%.
Above-mentioned substrate component is heated to 70-80 ℃, after fully mixing, carries out emulsifying, treat to add essence when temperature drops to 45 ℃ of left and right sides, rhGH stirs evenly, and promptly gets protective skin cream (having another name called the essence cream of whitening).
The preparation of embodiment 3 bath gel
Set of dispense ratio: 1-isobutyl-3,5-dimethylhexylphosphoric acid potassium salt 15%, ammonium lauryl sulfate 4%, cocamidopropyl propyl amide hydroxyl sulphur betanin 6%, dodecyl alcohol ether ammonium sulfate 8%, lauric acid amide of ethanol 3%, Pearl Concentrate 4%, essence 0.1%, citric acid transfers pH to 6.5-7.5, rhGH0.02%, and deionized water is added to 100%.
Above-mentioned substrate component is heated while stirring, treat that temperature arrives 70-80 ℃, fully behind the mixed dissolution, stop heating, when being cooled to 50 ℃ of left and right sides, add Pearl Concentrate, essence, rhGH, citric acid transfer pH to 6.5-7.5, stir, and promptly get bath gel.
The preparation of embodiment 4 cleansing milk
The set of dispense ratio: 1-isobutyl-3,5-dimethylhexylphosphoric acid potassium salt 30%, ammonium lauryl sulfate 8%, cocamidopropyl propyl amide hydroxyl sulphur betanin 6%, citric acid pH to 5.0-5.6, hydroxyethyl-cellulose 1.00%, glycerol 2.00%, rhGH 0.05%, and deionized water is added to 100%.
Above-mentioned substrate component is mixed, and heating treats that temperature arrives 70-80 ℃ while stirring, fully behind the mixed dissolution, stops heating, when being cooled to 50 ℃ of left and right sides, adds rhGH, adds citric acid and transfers pH to 5.0-5.6, stirs, and promptly gets cleansing milk.
The preparation of embodiment 5 eye creams
The set of dispense ratio: acrylic copolymer 0.35%, propylene glycol 4%, essence 0.1%, imidazolidinyl urea 0.05%, triethanolamine 10%, rhGH 0.001%, and deionized water is added to 100%.
Acrylic copolymer is uniformly dispersed in water, adds propylene glycol, be heated to 70-80 ℃, treat to add triethanol ammonium when temperature drops to 45 ℃ of left and right sides, the back that stirs adds essence, imidazolidinyl urea, and rhGH, stirring promptly gets eye cream.
The test of embodiment 6 senile rat skins coating skin care item
1, laboratory animal
Senile rat, the Wister strain, 18 months ages of Mus, about body weight 700 grams, male and female half and half.
2, reagent and dyestuff
All reagent and dyestuff are all available from Shanghai examination company or by the said firm's import packing.
3, experiment grouping
Every group of 10 senile rats (5 male Mus, 5 female Mus)
(1) matched group (I): skin is without any processing;
(2) matched group (II): skin coating commercial goods protective skin cream substrate;
(3) experimental group: skin coating contains recombinant human somatropin's's (content be ten thousand/ part by weight) protective skin cream (seeing embodiment 2 preparations).
4, experimental implementation
Rat neck back wool is cut off, expose 1cm
2Above skin applies secondary every day and is tried thing on the skin that exposes.Must clean with warm water earlier before the coating and dry skin, experimental session should in time shave off the Mus hair that newly grows, after four week of coating, and the execution rat.Use warm water cleaning skin, get the above skin of the coating about 0.5cm * 0.5cm in centre, and remove fat.Histological section's program routinely: fixing-dehydration-transparent-embedding-section-film-making.
Then, according to the Skin observing content, the section that will make respectively is through different dyeing courses:
A, hematoxylin (Hematoxylin) Yihong (Eosin) staining (being called for short the HE staining)
Dyeing course: section dewaxing-variable concentrations ethanol water inlet-washing-hematoxylin-washing-acidify-washing-weak ammonia-Yihong-washing-dehydration of alcohol-dimethylbenzene is transparent-mounting (consulting " pathological technique ", People's Health Publisher, 2000 years).
The HE coloration result: nucleus is blue, Cytoplasm, connective tissue, erythrocyte take on a red color (seeing Fig. 1,2).
B, dual compound staining method
Dyeing course: make section-dewaxing-water-70% ethanol-Victoria blue (VictoriaBlue)-95% alcohol-water to wash-Ponceau S (Poncean S)-100% ethanol-dry slightly-dimethylbenzene one mounting (consults " zoology magazine ", 26 (3): 29,1993).
Coloration result: elastic fiber is blue, collagen fiber take on a red color (seeing Fig. 3,4,5,6).
C, Immunohistochemical Staining
Dyeing course: frozen section-phosphate buffer-phosphate buffer (containing Triton * 100)-peroxidase quality liquid-Tris buffer (being terminated to colour response)-dehydration of alcohol-dimethylbenzene-mounting.
Coloration result: erythrocyte is dark-brown (seeing Fig. 7,8).
Claims (8)
1, a kind of cosmetics of nursing skin is characterized in that containing the recombinant human somatropin of one thousandth to ten ten thousand/part by weight amount, and the protein that described recombinant human somatropin is made up of 191 aminoacid, molecular weight are 22000 dalton.
2, a kind of preparation method of cosmetics of nursing skin, it is characterized in that mixing the recombinant human somatropin in the component of commercial goods cosmetics with the amount of one thousandth to ten ten thousand/part by weight, the protein that described recombinant human somatropin is made up of 191 aminoacid, molecular weight are 22000 dalton.
3, the cosmetics of nursing skin according to claim 1 is characterized in that described cosmetics comprise: protective skin cream, bath gel, eye cream, cleansing milk and massage cream.
4, as the cosmetics of nursing skin as described in the claim 3, it is characterized in that the component of described cosmetics protective skin cream and weight % content are as follows: monoglyceride 3%, sad triglyceride 2%, synthetic squalane 5%, dimethicone 1%, ten six octadecanol 3%, methyl hydroxybenzoate 0.2%, propylparaben 0.1%, glycerol 5%, isoparaffin/laureth-71%, essence 0.1%, the recombinant human somatropin 0.01%, water surplus.
5, as the cosmetics of nursing skin as described in the claim 3, the component and the weight % content that it is characterized in that described cosmetics bath gel are as follows: 1-isobutyl-3,5-dimethylhexylphosphoric acid potassium salt 15%, ammonium lauryl sulfate 4%, cocamidopropyl propyl amide hydroxyl sulphur betanin 6%, dodecyl alcohol ether ammonium sulfate 8%, lauric acid amide of ethanol 3%, Pearl Concentrate 4%, essence 0.1%, citric acid transfer pH to 6.5-7.5, the recombinant human somatropin 0.02%, water surplus.
6, as the cosmetics of nursing skin as described in the claim 3, the component and the weight % content that it is characterized in that described cosmetics eye cream are as follows: acrylic copolymer 0.35%, propylene glycol 4%, essence 0.1%, imidazolidinyl urea 0.05%, triethanolamine 10%, the recombinant human somatropin 0.001%, water surplus.
7, as the cosmetics of nursing skin as described in the claim 3, the component and the weight % content that it is characterized in that described cosmetics cleansing milk are as follows: 1-isobutyl-3,5-dimethylhexylphosphoric acid potassium salt 30%, ammonium lauryl sulfate 8%, cocamidopropyl propyl amide hydroxyl sulphur betanin 6%, citric acid pH to 5.0-5.6, hydroxyethyl-cellulose 1.00%, glycerol 2.00%, the recombinant human somatropin 0.05%, water surplus.
8, as the cosmetics of nursing skin as described in the claim 3, it is characterized in that the component of described cosmetics massage cream and weight % content are as follows: glyceryl monostearate 6.5%, ten six octadecanol 3.5%, No. 26 white oils 9%, sad triglyceride 4%, isopropyl palmitate 10%, dimethicone 2%, methyl hydroxybenzoate 0.2%, propylparaben 0.1%, triethanol ammonium 10%, sodium sulfite 0.1%, essence 0.1%, carbopol 940 5%, the recombinant human somatropin 0.1%, water surplus.
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| CN 03129040 CN1212112C (en) | 2003-06-04 | 2003-06-04 | Skin care cosmetics containing recombinant human auxin |
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| CN101450033B (en) * | 2007-12-04 | 2010-12-15 | 北京医力健生物科技有限公司 | Cosmetic of senility resistant composition and preparation method thereof |
| CN102366377A (en) * | 2011-05-19 | 2012-03-07 | 大连九羊食品有限公司 | Bath liquid containing sheep milk fat and preparation method thereof |
| CN102614103B (en) * | 2012-04-24 | 2013-06-12 | 黑龙江农垦东北黑蜂开发有限公司 | Middle and old-aged nourishing and wrinkle-resisting cream and preparation method thereof |
| CN103735458B (en) * | 2013-12-25 | 2016-08-24 | 珠海伊斯佳科技股份有限公司 | A kind of skin antiallergic maintenance compositions |
| CN111568781A (en) * | 2020-06-23 | 2020-08-25 | 上海美月生物科技有限公司 | Skin care gel and preparation method thereof |
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