CN119855824A - 核酸的制造方法、杂质的去除方法以及杂质少的核酸 - Google Patents

核酸的制造方法、杂质的去除方法以及杂质少的核酸 Download PDF

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Publication number
CN119855824A
CN119855824A CN202380064067.9A CN202380064067A CN119855824A CN 119855824 A CN119855824 A CN 119855824A CN 202380064067 A CN202380064067 A CN 202380064067A CN 119855824 A CN119855824 A CN 119855824A
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CN
China
Prior art keywords
nucleic acid
nucleophile
impurities
group
protecting group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380064067.9A
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English (en)
Chinese (zh)
Inventor
野村友美
四十庆佑
田中优贵
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Shokubai Co Ltd
Original Assignee
Nippon Shokubai Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Nippon Shokubai Co Ltd filed Critical Nippon Shokubai Co Ltd
Publication of CN119855824A publication Critical patent/CN119855824A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Saccharide Compounds (AREA)
CN202380064067.9A 2022-09-07 2023-09-01 核酸的制造方法、杂质的去除方法以及杂质少的核酸 Pending CN119855824A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022142150 2022-09-07
JP2022-142150 2022-09-07
PCT/JP2023/032085 WO2024053578A1 (ja) 2022-09-07 2023-09-01 核酸の製造方法、不純物の除去方法及び不純物が少ない核酸

Publications (1)

Publication Number Publication Date
CN119855824A true CN119855824A (zh) 2025-04-18

Family

ID=90191003

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380064067.9A Pending CN119855824A (zh) 2022-09-07 2023-09-01 核酸的制造方法、杂质的去除方法以及杂质少的核酸

Country Status (5)

Country Link
US (1) US20250361540A1 (https=)
EP (1) EP4585604A4 (https=)
JP (1) JPWO2024053578A1 (https=)
CN (1) CN119855824A (https=)
WO (1) WO2024053578A1 (https=)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6036493A (ja) * 1983-08-08 1985-02-25 Nippon Zeon Co Ltd 保護された核酸関連物質の分離法
JPH07227284A (ja) 1994-02-15 1995-08-29 Takaaki Oishi 合成ｄｎａの処理方法
DK1149109T3 (da) * 1999-02-05 2010-01-11 Ge Healthcare Bio Sciences Fremgangsmåde til afbeskyttelse af oligonukleotider
CN1643145A (zh) 2002-03-21 2005-07-20 艾夫西亚生物科技公司 寡核苷酸及其类似物的纯化方法
GB201115218D0 (en) 2011-09-02 2011-10-19 Glaxo Group Ltd Novel process
US10947262B2 (en) 2016-06-14 2021-03-16 Biogen Ma Inc. Hydrophobic interaction chromatography for purification of oligonucleotides
AU2020301419B2 (en) 2019-06-25 2026-03-19 Amgen Inc. Purification methods for carbohydrate-linked oligonucleotides
WO2021045141A1 (ja) * 2019-09-04 2021-03-11 神戸天然物化学株式会社 オリゴヌクレオチドの脱保護方法

Also Published As

Publication number Publication date
JPWO2024053578A1 (https=) 2024-03-14
EP4585604A1 (en) 2025-07-16
US20250361540A1 (en) 2025-11-27
WO2024053578A1 (ja) 2024-03-14
EP4585604A4 (en) 2026-04-08

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