CN1188518C - 利用阳离子表面活性剂稳定酶 - Google Patents

利用阳离子表面活性剂稳定酶 Download PDF

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Publication number
CN1188518C
CN1188518C CNB998088617A CN99808861A CN1188518C CN 1188518 C CN1188518 C CN 1188518C CN B998088617 A CNB998088617 A CN B998088617A CN 99808861 A CN99808861 A CN 99808861A CN 1188518 C CN1188518 C CN 1188518C
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CN
China
Prior art keywords
enzyme
reaction
tensio
polysaccharase
active agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CNB998088617A
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English (en)
Chinese (zh)
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CN1315996A (zh
Inventor
J·W·舒尔茨
F·黄
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Promega Corp
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Promega Corp
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Publication of CN1315996A publication Critical patent/CN1315996A/zh
Application granted granted Critical
Publication of CN1188518C publication Critical patent/CN1188518C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB998088617A 1998-06-24 1999-06-23 利用阳离子表面活性剂稳定酶 Expired - Lifetime CN1188518C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9053998P 1998-06-24 1998-06-24
US60/090,539 1998-06-24
US09/338,174 US6242235B1 (en) 1998-06-24 1999-06-22 Polymerase stabilization by polyethoxylated amine surfactants
US09/338,174 1999-06-22

Publications (2)

Publication Number Publication Date
CN1315996A CN1315996A (zh) 2001-10-03
CN1188518C true CN1188518C (zh) 2005-02-09

Family

ID=26782391

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB998088617A Expired - Lifetime CN1188518C (zh) 1998-06-24 1999-06-23 利用阳离子表面活性剂稳定酶

Country Status (12)

Country Link
US (1) US6242235B1 (fr)
EP (2) EP1918368A3 (fr)
JP (1) JP3673175B2 (fr)
CN (1) CN1188518C (fr)
AT (1) ATE363530T1 (fr)
AU (1) AU761757B2 (fr)
BR (1) BR9911396A (fr)
CA (1) CA2335153C (fr)
DE (1) DE69936201T2 (fr)
ES (1) ES2288317T3 (fr)
HK (1) HK1040262A1 (fr)
WO (1) WO1999067371A1 (fr)

Cited By (1)

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CN107075544A (zh) * 2014-07-22 2017-08-18 生物辐射实验室股份有限公司 与聚合酶联用的缓冲液

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DE19743518A1 (de) 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US20030134292A1 (en) * 2001-10-30 2003-07-17 Farchaus Joseph W. Thermostable DNA polymerases and methods of making same
US6814845B2 (en) * 2001-11-21 2004-11-09 University Of Kansas Method for depositing an enzyme on an electrically conductive substrate
EP1540009B1 (fr) * 2002-08-05 2011-09-28 Quanta Biosciences, Inc. Ameliorations apportees a des compositions pour l'amplification in vitro d'acides nucleiques
JP2006507818A (ja) * 2002-11-01 2006-03-09 プロメガ　コーポレイション 細胞溶解のための組成物、使用方法、装置およびキット
US20070054301A1 (en) * 2005-09-06 2007-03-08 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US20080050737A1 (en) * 2006-05-23 2008-02-28 Boaz Arieli Ambient Temperature Stable Kits for Molecular Diagnostics
WO2008013885A2 (fr) * 2006-07-25 2008-01-31 Stratagene California Détergents zwittérioniques pour le stockage et l'utilisation d'adn polymérases
US7846703B2 (en) * 2006-10-02 2010-12-07 Takara Bio Inc. Method for enhancing polymerase activity
CN101589157B (zh) * 2006-10-06 2014-04-02 万达利亚研究公司 用于连续快速热循环系统的方法
US7972828B2 (en) * 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
EP1970440A1 (fr) * 2007-03-06 2008-09-17 Qiagen GmbH Stabilisation de polymérase par des détergents ioniques
CA2689068A1 (fr) * 2007-06-13 2008-12-18 Ge Healthcare Bio-Sciences Corp. Stabilisation de polymerases
US20110014676A1 (en) * 2007-06-29 2011-01-20 Battelle Memorial Institute Protein stabilization
EP2075331A1 (fr) * 2007-12-28 2009-07-01 Qiagen GmbH Enzymes modifiées et leurs utilisations
US20090203022A1 (en) * 2008-02-07 2009-08-13 Arizona Board Of Regents For And On Behalf Of Arizona State University Analysis
US20120111727A1 (en) * 2009-01-26 2012-05-10 Vladislav Dolnik Capillary sieving electrophoresis with a cationic surfactant for size separation of proteins
EP2393941A2 (fr) * 2009-02-09 2011-12-14 Frederic Zenhausern Améliorations se rapportant à une analyse
GB0915796D0 (en) 2009-09-09 2009-10-07 Fermentas Uab Polymerase compositions and uses
CN102051352B (zh) * 2009-11-02 2012-07-04 刘萍 一种酶结合物稳定液
WO2012018638A2 (fr) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions de stabilisation d'adn, d'arn, de protéines dans le sang et d'autres échantillons biologiques lors du transport et du stockage à températures ambiantes
WO2012018639A2 (fr) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions de stabilisation d'adn, d'arn, de protéines salivaires et d'autres échantillons biologiques lors du transport et du stockage à températures ambiantes
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
WO2012170907A2 (fr) 2011-06-08 2012-12-13 Life Technologies Corporation Polymérisation d'acides nucléiques utilisant des protéines ayant des points isoélectriques faibles
CN103796987B (zh) 2011-06-08 2016-09-21 生命技术公司 用于pcr系统的新型去污剂的设计和开发
WO2013006793A2 (fr) * 2011-07-06 2013-01-10 Quest Diagnostics Investments Incorporated Amplification directe et détection d'agents pathogènes viraux et bactériens
CN102559647A (zh) * 2011-12-31 2012-07-11 湖南利尔康生物有限公司 提高液体酶应用效果的方法
KR102078892B1 (ko) * 2012-02-09 2020-02-19 라이프 테크놀로지스 코포레이션 접합된 중합체성 입자 및 그의 제조 방법
US8956816B2 (en) 2012-06-05 2015-02-17 Pacific Biosciences Of California, Inc. Methods and compositions for performing analytical operations
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CN107922974B (zh) 2015-07-02 2021-11-09 生命技术公司 羧基官能亲水微珠的偶合
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US11268084B2 (en) 2017-08-08 2022-03-08 Thermo Fisher Scientific Baltics, UAB Glycerol-free formulations for reverse transcriptases
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JP2021504546A (ja) * 2017-11-29 2021-02-15 ビーエイエスエフ・ソシエタス・エウロパエアＢａｓｆ Ｓｅ 組成物、その製造方法および使用方法

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107075544A (zh) * 2014-07-22 2017-08-18 生物辐射实验室股份有限公司 与聚合酶联用的缓冲液
CN107075544B (zh) * 2014-07-22 2021-01-29 生物辐射实验室股份有限公司 与聚合酶联用的缓冲液

Also Published As

Publication number Publication date
US6242235B1 (en) 2001-06-05
JP2004500005A (ja) 2004-01-08
ES2288317T3 (es) 2008-01-01
CA2335153A1 (fr) 1999-12-29
AU761757B2 (en) 2003-06-12
EP1918368A3 (fr) 2008-06-18
BR9911396A (pt) 2002-01-29
ATE363530T1 (de) 2007-06-15
DE69936201T2 (de) 2008-02-07
CA2335153C (fr) 2007-04-10
DE69936201D1 (de) 2007-07-12
AU4829399A (en) 2000-01-10
CN1315996A (zh) 2001-10-03
WO1999067371A1 (fr) 1999-12-29
JP3673175B2 (ja) 2005-07-20
EP1088060A1 (fr) 2001-04-04
EP1918368A2 (fr) 2008-05-07
EP1088060B1 (fr) 2007-05-30
HK1040262A1 (en) 2002-05-31
EP1088060A4 (fr) 2004-12-01

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Granted publication date: 20050209