CN117899167A - Anti-inflammatory and antibacterial traditional Chinese medicine composition and preparation method thereof - Google Patents
Anti-inflammatory and antibacterial traditional Chinese medicine composition and preparation method thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention provides an anti-inflammatory and antibacterial traditional Chinese medicine composition and a preparation method thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises the following components: astragalus root, bletilla tuber, gentian root, rush, phellodendron bark, chinese gall and honeysuckle flower; the preparation method comprises (1) mixing radix astragali with alkaline organic solvent, and reflux extracting to obtain radix astragali extractive solution and radix astragali residue; (2) Mixing the radix astragali residue with rhizoma Bletillae, medulla Junci, and cortex Phellodendri, soaking, and decocting to obtain mixed extractive solution and water decoction residue; (3) Mixing the decoction dregs with the rest components, mixing with weak acid organic solvent, performing ultrasonic treatment, and reflux-extracting to obtain extractive solution and medicinal residue extracted by acid organic solvent solution; (4) Mixing the extractive solutions, concentrating or drying to obtain Chinese medicinal composition. The traditional Chinese medicine composition has obvious antibacterial and anti-inflammatory effects, is used for preparing preparations with anti-inflammatory and antibacterial effects, has obvious antibacterial and anti-inflammatory effects, and is safe and nontoxic.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an anti-inflammatory and antibacterial traditional Chinese medicine composition and a preparation method thereof.
Background
The skin has the important functions of resisting microbial invasion, excreting, preventing water evaporation, regulating question, maintaining the stable environment in the body, etc. However, the skin, the oral cavity and the scalp are inevitably damaged, and wounds are caused. However, if the wound is not treated in time, bacteria and fungi often grow, and severe wounds can even cause inflammation, ulceration, abscess, canker sores and the like. Therefore, in the process of wound healing, intervention is performed in time, medicines are smeared, the wound healing can be promoted, bacteria inhibition and sterilization can be realized, and wound infection is avoided or controlled.
Common bacteria that cause wound infections typically include staphylococcus aureus, staphylococcus epidermidis, escherichia coli, and pseudomonas aeruginosa. Staphylococcus aureus and pseudomonas aeruginosa are usually parasitic on the skin, nasal cavity, throat and other parts of human body, and if the wound part is damaged, the wound part can be infected, thereby causing local red swelling, pain, purulence and other symptoms. Coli is a bacterium parasitic in the human intestinal tract and usually causes diarrhea, abdominal pain, nausea and other symptoms. If the wound part of the patient is damaged, the infection of the escherichia coli can be caused, and the symptoms such as local skin redness, pain, purulence and the like are caused.
Currently, commonly used antibacterial agents include silver-based, iodine-based, organic molecular antibacterial agents, antibiotics, and the like. However, silver is expensive and tends to cause heavy metal residue; the stability of iodine is poor, and the irritation to skin and mucous membrane is strong; the organic micromolecular antibacterial agent has poor antibacterial broad spectrum and antibacterial effect; antibiotics have been increasingly limited in use in recent years. In addition, although the antibacterial agent can be used for disinfection treatment, the antibacterial agent cannot be used for oral administration or gargling, and damage to human bodies or animal bodies is caused after mistaking. In addition, when the wound is infected, inflammation and redness are often caused, and the above antibacterial agent does not have anti-inflammatory efficacy, but rather aggravates inflammation.
The traditional Chinese medicine and the active ingredients thereof have the advantages of natural taking, small toxic and side effects, no drug residue and drug resistance, no harm to health, high efficiency, safety and the like, can solve the problems of drug resistance, irritation, no anti-inflammatory effect or poor anti-inflammatory effect, allergy or destroyed microecological balance of pathogenic bacteria caused by chemical synthetic drugs such as antibiotics and the like, and have important application value in the aspect of preparation of anti-inflammatory bacteriostats. The anti-inflammatory and antibacterial studies of traditional Chinese medicines have been carried out for the past year, such as the Licorice heart-purging decoction in jin Kui Yao Lloyd, which is prepared from radix Scutellariae and rhizoma Bletillae, and can be used for treating canker sore. Chinese patent CN112870293A discloses a Chinese medicinal composition for trauma and its application, which is prepared from gallnut 10-30 parts, rhubarb 10-30 parts, bletilla tuber 10-15 parts, mirabilite 10-20 parts, humifuse euphorbia herb 10-15 parts, dragon's blood powder 2-5 parts, notoginseng powder 3-6 parts, and can achieve the effects of rapid hemostasis, pain relieving and bacteriostasis. The Chinese patent CN102114216A is an external application antibacterial and anti-inflammatory medicament and a preparation method thereof, and the medicament is prepared from raw materials of peppermint, schizonepeta, ledebouriella root, rhubarb, phellodendron bark, common andrographis herb, weeping forsythiae capsule, baical skullcap root, bunge corydalis herb, danshen root, lightyellow sophora root, dahurian angelica root, tree peony bark, aloe, astragalus root, dyers woad leaf, sweet wormwood herb, gentian, sinkiang arnebia root, liquorice, chinese gall, coptis root, cassia seed, indigowoad root, honeysuckle flower, chrysanthemum, hawthorn, stemona root, mugwort leaf, common cnidium fruit, borneol powder, borax powder, honey, glycerol, propylene glycol, tween 60, OP10, castor oil and turpentine. The obtained product has good antibacterial and anti-inflammatory effects, the bacteria can not generate antibodies and have no drug resistance, the bacteria are prevented from being mutated into super bacteria, no toxic or side effect is caused, and the use is safe. However, the components of the medicament are 39 kinds in total, the components are complex, the preparation is troublesome, and the medicament has no universality.
In addition, most of the traditional Chinese medicine components are used as anti-inflammatory bacteriostats at present, the effective components are simply overlapped or simply combined with active substances to be applied, the synergistic effect of the traditional Chinese medicine or the active components is ignored, and the influence of an extraction method on the technical effect is ignored. Therefore, the traditional Chinese medicine components and the preparation method of the anti-inflammatory and antibacterial preparation are researched, and the anti-inflammatory and antibacterial traditional Chinese medicine composition which has reasonable formula collocation, simple formula, reasonable preparation method, obvious synergistic anti-inflammatory and antibacterial effects, safety, no toxicity, capability of being used for external application and gargling and wide application range is developed, and has important significance.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides an anti-inflammatory and antibacterial traditional Chinese medicine composition and a preparation method thereof, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine components: the astragalus, bletilla, gentian, rush, phellodendron, chinese gall and honeysuckle are extracted by a specific extraction method, and the components are mutually matched in a synergistic way, so that the growth of escherichia coli, staphylococcus aureus and pseudomonas aeruginosa can be obviously inhibited, the swelling of mouse ears caused by dimethylbenzene is obviously inhibited, and the level of tumor necrosis factor TNF-alpha in rat serum is reduced. The traditional Chinese medicine composition is applied to the preparation of products such as lotion, toothpaste, mouthwash, cosmetics, traumatic pharmaceutical preparations and the like, and has remarkable anti-inflammatory and antibacterial effects.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
Firstly, the invention provides an anti-inflammatory and antibacterial traditional Chinese medicine composition, which comprises the following components: astragalus root, bletilla tuber, gentian root, rush, phellodendron bark, chinese gall and honeysuckle flower.
Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 10-16 parts of astragalus, 3-10 parts of bletilla, 3-8 parts of gentian, 1.5-6 parts of rush, 8-18 parts of phellodendron, 5-15 parts of gallnut and 3-8 parts of honeysuckle.
Further preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 11-15 parts of astragalus, 4-7 parts of bletilla, 4-7 parts of gentian, 2-5 parts of rush, 10-15 parts of phellodendron, 6-10 parts of gallnut and 4-6 parts of honeysuckle.
Still more preferably, the Chinese medicinal composition comprises the following components in parts by weight: 13 parts of astragalus, 6 parts of bletilla, 5 parts of gentian, 3 parts of rush, 12 parts of phellodendron, 8 parts of gallnut and 5 parts of honeysuckle.
Preferably, the weight ratio of the astragalus, the gentian, the gallnut and the honeysuckle is 10-16:3-8:5-15:3-8; further preferably, the weight ratio of the astragalus, the gentian, the gallnut and the honeysuckle is 11-15:4-7:6-10:4-6; still more preferably, the weight ratio of the astragalus, the gentian, the gallnut and the honeysuckle is 13:5:8:5.
Then, the invention provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
(1) Pulverizing radix astragali, mixing with alkaline organic solvent, and reflux extracting to obtain radix astragali extractive solution and radix astragali residue;
(2) Mixing the radix astragali residue with rhizoma Bletillae, medulla Junci, and cortex Phellodendri, soaking in water, and decocting to obtain decoction mixture and water decoction residue;
(3) Mixing the decoction dregs with gentian, chinese gall and honeysuckle, crushing, mixing with a weak acid organic solvent solution, then carrying out ultrasonic treatment, and finally carrying out reflux extraction to obtain an extracting solution extracted by the acid organic solvent solution and medicinal herb dregs;
(4) Mixing the astragalus extract in the step (1), the decoction mixed solution in the step (2) and the extract extracted by the acidic organic solvent solution in the step (3), and concentrating or drying to obtain the traditional Chinese medicine composition.
Preferably, in step (1), the pulverization is carried out with a particle size of 80 to 200 mesh.
Preferably, in step (1), the organic solvent is at least one selected from ethanol, methanol, butanol, and diethyl ether.
Further preferably, the organic solvent is at least one selected from ethanol and butanol.
Preferably, in step (1), the alkali in the alkaline organic solvent is at least one selected from sodium carbonate, sodium bicarbonate, food grade sodium hydroxide, and food grade potassium hydroxide, so as to adjust the pH of the organic solvent to be weak alkaline.
Preferably, in step (1), the basic organic solvent has a pH of 8.5 to 10.0.
Further preferably, the basic organic solvent has a pH of 8.8 to 9.5.
Still more preferably, the basic organic solvent has a pH of 9.0.
Preferably, in the step (1), the volume concentration of the organic solvent is 65-95%, and the solid-to-liquid ratio of the astragalus to the organic solvent is 1:8-16g/mL.
Further preferably, the volume concentration of the organic solvent is 75-85%, and the solid-liquid ratio of the astragalus to the organic solvent is 1:10-15g/mL.
Still more preferably, the volume concentration of the organic solvent is 82%, and the solid-to-liquid ratio of astragalus to the organic solvent is 1:12g/mL.
Preferably, in the step (1), the number of times of reflux extraction is 2-4, the temperature of each extraction is 55-80 ℃, the extraction time is 30-120min, filtering, combining the extracting solutions of each extraction, then adjusting the pH to be neutral, and concentrating under reduced pressure to remove the organic solvent, thus obtaining the astragalus extract and astragalus dregs.
Further preferably, the reflux extraction is carried out for 2-3 times, the temperature of each extraction is 58-70 ℃, the extraction time is 45-90min, the filtration is carried out, the extracting solutions of each extraction are combined, the pH is regulated to be neutral by citric acid, and the organic solvent is removed by decompression concentration, thus obtaining astragalus extract and astragalus dregs.
Still more preferably, the reflux extraction is carried out for 2 times, the temperature of each extraction is 65 ℃, the extraction time is 60min, the filtration is carried out, the extraction solutions obtained by 2 times of extraction are combined, then the pH is regulated to be neutral by 0.5-2% of citric acid, and the organic solvent is removed by decompression concentration, thus obtaining astragalus extract and astragalus dregs.
Preferably, in the step (2), soaking is carried out by adding water for 1-5h, and the solid-liquid ratio is 1:15-25g/mL; the solid-liquid ratio is the ratio of the total mass of the medicinal materials of astragalus, bletilla striata, rush and phellodendron bark to water.
Further preferably, the soaking time is 2-5h, and the solid-to-liquid ratio is 1:18-20g/mL.
Preferably, in the step (2), the times of decoction are 1-3 times, each time of decoction is 0.5-2 hours, the solid-liquid ratio of each time of decoction is the same, the decoction is filtered, and the decoction is combined to obtain a decoction mixed solution and water decoction dregs.
Further preferably, the number of times of decoction is 3, specifically: the first time of decoction is 1h, the second time of decoction is 1.5h, and the third time of decoction is 0.5h; filtering, and mixing the decoctions to obtain decoction mixture and water decoction dregs.
Preferably, in the step (3), the pulverization is carried out with a particle size of 50 to 100 mesh.
Preferably, in step (3), the organic solvent in the weakly acidic organic solvent solution is at least one selected from ethanol, acetone, methanol, and ethyl acetate.
Further preferably, the organic solvent is at least one selected from ethanol, acetone, and methanol.
Preferably, in the step (3), the acid in the weak acid organic solvent solution is at least one selected from citric acid, acetic acid, malic acid, tartaric acid and phosphoric acid, so as to adjust the pH of the organic solvent to be weak acid.
Further preferably, the acid in the weakly acidic organic solvent solution is at least one selected from the group consisting of citric acid, acetic acid, malic acid, and phosphoric acid.
Preferably, in step (3), the weak acid organic solvent solution has a pH of 4.5 to 6.8.
Further preferably, the pH of the weakly acidic organic solvent solution is 4.8-6.5.
Still more preferably, the weak acid organic solvent solution has a pH of 5 to 5.5.
Preferably, in the step (3), the volume concentration of the organic solvent solution is 65-85%, and the solid-to-liquid ratio of the traditional Chinese medicine to the organic solvent solution is 1:8-16g/mL; the Chinese medicine is all Chinese medicine components.
Further preferably, the volume concentration of the organic solvent solution is 70-80%, and the solid-to-liquid ratio of the traditional Chinese medicine to the organic solvent solution is 1:10-15g/mL.
Still more preferably, the volume concentration of the organic solvent solution is 75%, and the solid-to-liquid ratio of the traditional Chinese medicine to the organic solvent solution is 1:12g/mL.
Preferably, in the step (3), the ultrasonic treatment is carried out for 20-50min, and the ultrasonic frequency is 60-100kHz.
Further preferably, the ultrasonic treatment is carried out for 30-40min, and the ultrasonic frequency is 80kHz.
Preferably, in the step (3), the number of times of reflux extraction is 2-3, the temperature of each extraction is 55-80 ℃, the extraction time is 30-90min, filtering, combining the extracting solutions of each extraction, and concentrating under reduced pressure to remove the organic solvent, thereby obtaining the extracting solution extracted by the acidic organic solvent solution and medicinal herb residues.
Further preferably, the reflux extraction is carried out for 2 times, the temperature of each extraction is 60-70 ℃, the time of each extraction is 40-50min, the filtration is carried out, the extracting solutions of each extraction are combined, the organic solvent is removed by decompression concentration, and the extracting solution and the medicinal herb residue extracted by the acid organic solvent solution are obtained.
Still more preferably, the reflux extraction is carried out for 2 times, the temperature of each extraction is 60-70 ℃, the time of each extraction is 45min, the filtration is carried out, the extracting solutions of each extraction are combined, then the pH is regulated to 6-7 by 0.1-1% sodium hydroxide, and the organic solvent is removed by decompression concentration, thus obtaining the extracting solution extracted by the acid organic solvent solution and the medicinal herb residue.
Preferably, in the step (4), the mixing may be further performed with a low-temperature sterilization treatment after the mixing.
Furthermore, the invention provides application of the traditional Chinese medicine composition in preparation of a preparation with anti-inflammatory and antibacterial effects.
Finally, the invention provides a preparation with anti-inflammatory and antibacterial effects, which comprises the traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials.
Preferably, the formulation includes, but is not limited to, a lotion, toothpaste, mouthwash, cosmetic, and traumatic pharmaceutical spread.
In the invention, the astragalus is mainly used for treating warm diseases, upper respiratory tract inflammation, pneumonia, jaundice, dysentery and the like, and the astragalus can be used for external use to help relieve inflammation and infection symptoms of furuncle by anti-inflammation and antibacterial effects. The active ingredients such as saponin and flavonoid in radix astragali have antiinflammatory effect, and can reduce inflammation and relieve pain.
In the invention, the bletilla striata is bitter, sweet, astringent and slightly cold in taste. It enters lung, liver and stomach meridians. Has effects of astringing, stopping bleeding, detumescence and promoting granulation. The rhizoma bletillae can promote tissue regeneration and treat sores in the book of materia medica, and the modern pharmacological research also proves that the traditional Chinese medicine has good effects of resisting bacteria, diminishing inflammation and stopping bleeding. Can be used for treating hemoptysis, hematemesis, traumatic hemorrhage, pyocutaneous disease, toxic swelling, and chapped skin.
In the invention, the gentian is a rhizome part of gentian Gentiana scabra Bunge which is a gentian of gentiaceae, and the extract thereof has different degrees of inhibition effects on pseudomonas aeruginosa, bacillus proteus, typhoid bacillus, bacillus dysenteriae, staphylococcus aureus and the like.
In the invention, the rush is slightly cold in nature and taste, sweet and light; it is effective in clearing away heart fire, promoting urination, and reducing pathogenic fire. The rush is rich in dihydric phenols, dehydrorush diphenol and other antibacterial bioactive components.
In the invention, the phellodendron bark has an inhibitory effect on various pathogenic infectious bacteria, such as staphylococcus, hemolytic streptococcus, gonococcus, typhoid bacillus, shigella dysenteriae, pneumococcus, cholera solitary fungus, bacillus subtilis, meningococcus and the like.
In the invention, gallic acid, astringency and cold enter lung, large intestine and kidney meridian. Astringing lung to reduce fire, astringing intestine to check diarrhea, arresting sweating, stopping bleeding, astringing dampness to arrest sore. Can be used for treating cough due to lung deficiency, cough due to lung heat and phlegm, chronic diarrhea and dysentery, spontaneous perspiration and night sweat, diabetes, hematochezia, hemorrhoid, traumatic hemorrhage, carbuncle, swelling, and skin ulcer.
In the invention, the honeysuckle has an inhibiting effect on staphylococcus aureus, staphylococcus albus, streptococcus hemolyticus, bacillus pneumoniae, neisseria meningitidis, bacillus typhi and other proteus, pertussis bacillus and gram-positive and negative bacteria, and has the effects of clearing heat and detoxicating, dredging channels and activating collaterals, diminishing inflammation and detoxifying.
Although four traditional Chinese medicines of astragalus, gentian, gallnut and honeysuckle have certain antibacterial effect, the antibacterial effect is still limited when the four traditional Chinese medicines are singly or in a compound way of two or three; according to the invention, when the ratio of the four traditional Chinese medicines of astragalus, gentian, gallnut and honeysuckle is 10-16:3-8:5-15:3-8, especially 11-15:4-7:6-10:4-6, the traditional Chinese medicine composition and the preparation thereof extracted by a specific method have the best antibacterial effect on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, and have remarkable synergistic effect and antibacterial effect improvement effects; and has remarkable anti-inflammatory effect.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention performs screening and synergistic compatibility on the traditional Chinese medicine components to obtain the components: the traditional Chinese medicine composition of astragalus, bletilla, gentian, rush, phellodendron, chinese gall and honeysuckle is mutually synergistic, and can obviously improve the diameter of a bacteriostasis zone of escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, and the bacteria are extremely or highly sensitive to the traditional Chinese medicine composition; the traditional Chinese medicine composition has remarkable inhibition effect on mouse ear swelling caused by dimethylbenzene, can remarkably reduce the level of tumor necrosis factor TNF-alpha in rat serum, and has excellent antibacterial and anti-inflammatory effects.
2. The invention comprehensively utilizes the synergistic effect among the traditional Chinese medicines, has definite components of the traditional Chinese medicine composition, reasonable collocation, is beneficial to absorption, and plays the role of each active component with high efficiency; the traditional Chinese medicine composition obtained by the specific ingredient matching and extraction method has high activity and high ingredient utilization rate, and can efficiently inhibit bacteria and resist inflammation.
3. The traditional Chinese medicine composition is safe, nontoxic, delicious and wide in application range, and can be used in the fields of medicine and cosmetics; the raw materials have simple and easily obtained components, clear components and high-efficiency antibacterial and anti-inflammatory effects.
Detailed Description
The following non-limiting examples will enable those of ordinary skill in the art to more fully understand the invention and are not intended to limit the invention in any way. The following is merely exemplary of the scope of the invention as it is claimed and many variations and modifications of the invention will be apparent to those skilled in the art in light of the disclosure, which should be considered as falling within the scope of the invention as claimed. Where numerical ranges are provided in the examples, it is understood that unless otherwise stated herein, both endpoints of each numerical range and any number between the two endpoints are significant both in the numerical range. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
The invention is further illustrated by means of the following specific examples. The various chemical reagents used in the examples of the present invention were obtained by conventional commercial means unless otherwise specified. The components of the invention have no obvious influence on the effect of products of different factories.
Example 1
An anti-inflammatory and antibacterial traditional Chinese medicine composition comprises the following components in parts by weight: 13 parts of astragalus, 6 parts of bletilla, 5 parts of gentian, 3 parts of rush, 12 parts of phellodendron, 8 parts of gallnut and 5 parts of honeysuckle.
In this embodiment, the preparation method of the traditional Chinese medicine composition includes the following steps:
(1) Pulverizing radix astragali, mixing with alkaline ethanol solution containing sodium carbonate with pH of 9.0, and reflux extracting, wherein the volume concentration of ethanol in the ethanol solution is 82%, and the solid-liquid ratio of radix astragali to ethanol solution is 1:12g/mL; in the reflux extraction, the times of reflux extraction are 2 times, the temperature of each extraction is 65 ℃, the time of each extraction is 60min, filtering, combining the extracting solutions of 2 times, then adjusting the pH to be neutral by using 1.5% citric acid, and concentrating under reduced pressure to remove the organic solvent to obtain astragalus extract and astragalus dregs;
(2) Mixing the residue of radix astragali with rhizoma Bletillae, medulla Junci, and cortex Phellodendri, soaking in 20 times of water for 3 hr; then decocting for 3 times: the first time of decoction is 1h, the second time of decoction is 1.5h, and the third time of decoction is 0.5h; filtering after each decoction, and mixing the decoctions to obtain a decoction mixture and water decoction dregs;
(3) Mixing the decoction dregs with radix Gentianae, galla chinensis and flos Lonicerae, pulverizing into 100 mesh, mixing with ethanol solution containing citric acid and acetic acid (citric acid: acetic acid=1:1, volume ratio) with pH of 5.3, controlling solid-liquid ratio of the Chinese medicinal materials and ethanol solution to 1:12g/mL, and volume concentration of ethanol solution to 75%; after mixing, carrying out ultrasonic treatment with the frequency of 80kHz for 35min; finally, carrying out reflux extraction for 2 times, wherein the temperature of each extraction is 65 ℃, the time of each extraction is 45min, filtering after each reflux extraction, combining the extracting solutions of each extraction, then adjusting the pH to 7 by using 0.5% sodium hydroxide, and concentrating under reduced pressure to remove the organic solvent, thereby obtaining extracting solution extracted by the acid organic solvent solution and medicinal herb residues;
(4) Mixing the astragalus extract in the step (1), the decoction mixed solution in the step (2) and the extract extracted by the acidic organic solvent solution in the step (3), concentrating and drying at low temperature to obtain the traditional Chinese medicine composition.
Example 2
An anti-inflammatory and antibacterial traditional Chinese medicine composition comprises the following components in parts by weight: 11 parts of astragalus, 4 parts of bletilla, 4 parts of gentian, 2 parts of rush, 10 parts of phellodendron, 6 parts of gallnut and 4 parts of honeysuckle.
In this embodiment, the preparation method of the traditional Chinese medicine composition includes the following steps:
(1) Pulverizing radix astragali, with a particle size of 200 meshes, mixing with alkaline ethanol solution containing food-grade sodium hydroxide with a pH of 8.8, and reflux-extracting, wherein the volume concentration of ethanol in the ethanol solution is 85%, and the solid-liquid ratio of radix astragali to the ethanol solution is 1:10g/mL; in the reflux extraction, the times of reflux extraction are 2 times, the temperature of each extraction is 58 ℃, the time of each extraction is 90min, the extraction solutions of 2 times are filtered, the pH of the extraction solutions is regulated to be neutral by using 1.5 percent of citric acid, and the organic solvent is removed by decompression concentration, so as to obtain astragalus extract and astragalus dregs;
(2) Mixing the residue of radix astragali with rhizoma Bletillae, medulla Junci, and cortex Phellodendri, soaking in 18 times of water for 5 hr; then decocting for 3 times, each time for 1h; filtering after each decoction, and mixing the decoctions to obtain a decoction mixture and water decoction dregs;
(3) Mixing the decoction dregs with gentian, chinese gall and honeysuckle, crushing the mixture into particles with the particle size of 80 meshes, mixing the particles with an ethanol solution containing acetic acid with the pH of 5.0, and controlling the solid-to-liquid ratio of the traditional Chinese medicine to the ethanol solution to be 1:10g/mL and the volume concentration of the ethanol solution to be 70%; after mixing, carrying out ultrasonic treatment with the frequency of 60kHz for 40min; finally, carrying out reflux extraction for 2 times, wherein the temperature of each extraction is 60 ℃, the time of each extraction is 50min, filtering after each reflux extraction, combining the extracting solutions of each extraction, then adjusting the pH to 7 by using 0.5% sodium hydroxide, and concentrating under reduced pressure to remove the organic solvent, thereby obtaining extracting solution extracted by the acid organic solvent solution and medicinal herb residues;
(4) Mixing the astragalus extract in the step (1), the decoction mixed solution in the step (2) and the extract extracted by the acidic organic solvent solution in the step (3), concentrating and drying at low temperature to obtain the traditional Chinese medicine composition.
Example 3
An anti-inflammatory and antibacterial traditional Chinese medicine composition comprises the following components in parts by weight: 15 parts of astragalus, 7 parts of bletilla, 7 parts of gentian, 5 parts of rush, 15 parts of phellodendron, 10 parts of gallnut and 6 parts of honeysuckle.
In this embodiment, the preparation method of the traditional Chinese medicine composition includes the following steps:
(1) Pulverizing radix astragali, mixing with alkaline ethanol solution containing food-grade sodium hydroxide with pH of 9.5, and reflux extracting, wherein the volume concentration of ethanol in the ethanol solution is 75%, and the solid-liquid ratio of radix astragali to ethanol solution is 1:15g/mL; in the reflux extraction, the times of reflux extraction are 2 times, the temperature of each extraction is 70 ℃, the time of each extraction is 45min, the extraction is filtered, the extraction solutions of 2 times are combined, then the pH is regulated to be neutral by using 1.5 percent of citric acid, and the organic solvent is removed by decompression concentration, so as to obtain astragalus extract and astragalus dregs;
(2) Mixing the residue of radix astragali with rhizoma Bletillae, medulla Junci, and cortex Phellodendri, soaking in 25 times of water for 2 hr; then decocting for 2 times, each time for 2 hours; filtering after each decoction, and mixing the decoctions to obtain a decoction mixture and water decoction dregs;
(3) Mixing the decoction dregs with gentian, chinese gall and honeysuckle, crushing the mixture into particles with the particle size of 50 meshes, mixing the particles with an ethanol solution containing citric acid with the pH of 4.8, and controlling the solid-to-liquid ratio of the traditional Chinese medicine to the ethanol solution to be 1:16g/mL and the volume concentration of the ethanol solution to be 80%; after mixing, carrying out ultrasonic treatment with the frequency of 100kHz for 30min; finally, carrying out reflux extraction for 3 times, wherein the temperature of each extraction is 55 ℃, the time of each extraction is 40min, filtering after each reflux extraction, combining the extracting solutions of each extraction, and concentrating under reduced pressure to remove the organic solvent to obtain extracting solution extracted by the acidic organic solvent solution and medicinal herb residues;
(4) Mixing the astragalus extract in the step (1), the decoction mixed solution in the step (2) and the extract extracted by the acidic organic solvent solution in the step (3), concentrating and drying at low temperature to obtain the traditional Chinese medicine composition.
Example 4
An anti-inflammatory and antibacterial traditional Chinese medicine composition comprises the following components in parts by weight: 10 parts of astragalus, 3 parts of bletilla, 3 parts of gentian, 1.5 parts of rush, 8 parts of phellodendron, 5 parts of gallnut and 3 parts of honeysuckle.
In this example 4, the preparation method of the Chinese medicinal composition is different from example 1 in that in step (1), the ethanol solution is replaced with a butanol solution. The rest steps are the same, and the traditional Chinese medicine composition is prepared.
Example 5
An anti-inflammatory and antibacterial traditional Chinese medicine composition comprises the following components in parts by weight: 16 parts of astragalus, 10 parts of bletilla, 8 parts of gentian, 6 parts of rush, 18 parts of phellodendron, 15 parts of gallnut and 8 parts of honeysuckle.
In this example 5, the preparation method of the traditional Chinese medicine composition is different from example 1 in that the step (3) is different, specifically, the parameter settings of the ultrasonic treatment and the reflux extraction are different:
(1) - (2) as in example 1;
(3) Mixing the decoction dregs with radix Gentianae, galla chinensis and flos Lonicerae, pulverizing into 100 mesh, mixing with ethanol solution containing citric acid and acetic acid (citric acid: acetic acid=1:1, volume ratio) with pH of 6.5, controlling solid-liquid ratio of the Chinese medicinal materials and ethanol solution to 1:12g/mL, and volume concentration of ethanol solution to 85%; after mixing, carrying out ultrasonic treatment with the frequency of 80kHz for 35min; finally, carrying out reflux extraction for 2 times, wherein the temperature of each extraction is 65 ℃, the time of each extraction is 45min, filtering after each reflux extraction, combining the extracting solutions of each extraction, then adjusting the pH to 7 by using 0.5% sodium hydroxide, and concentrating under reduced pressure to remove the organic solvent, thereby obtaining extracting solution extracted by the acid organic solvent solution and medicinal herb residues;
(4) As in example 1.
Comparative example 1
Unlike example 1, the Chinese medicinal composition has different components, does not contain astragalus, and comprises the following components in parts by weight: 6 parts of rhizoma bletillae, 5 parts of gentian, 3 parts of rush, 12 parts of phellodendron, 8 parts of gallnut and 5 parts of honeysuckle.
The preparation method of the traditional Chinese medicine composition is the same as that of the example 1.
Comparative example 2
Unlike example 1, the Chinese medicinal composition is different in composition, does not contain gallnut, and comprises the following components in parts by weight: 13 parts of astragalus, 6 parts of bletilla, 5 parts of gentian, 3 parts of rush, 12 parts of phellodendron and 5 parts of honeysuckle.
The preparation method of the traditional Chinese medicine composition is the same as that of the example 1.
Comparative example 3
Unlike example 1, the composition of the Chinese medicine is different in composition, and does not contain astragalus and gallnut. Specifically, the composition comprises the following components in parts by weight: 6 parts of bletilla striata, 5 parts of gentian, 3 parts of rush, 12 parts of phellodendron and 5 parts of honeysuckle.
The preparation method of the traditional Chinese medicine composition is the same as that of the example 1.
Comparative example 4
Unlike example 1, the composition of the traditional Chinese medicine was different in that honeysuckle was replaced with pseudo-ginseng. Specifically, the composition comprises the following components in parts by weight: 13 parts of astragalus, 6 parts of bletilla, 5 parts of gentian, 3 parts of rush, 12 parts of phellodendron, 8 parts of gallnut and 5 parts of pseudo-ginseng.
The preparation method of the traditional Chinese medicine composition is the same as that of the example 1.
Comparative example 5
Unlike example 1, the traditional Chinese medicine composition comprises the following components in parts by weight: 24 parts of astragalus, 6 parts of bletilla, 2 parts of gentian, 3 parts of rush, 12 parts of phellodendron, 2 parts of gallnut and 2 parts of honeysuckle.
The preparation method of the traditional Chinese medicine composition is the same as that of the example 1.
Comparative example 6
Unlike example 1, the preparation method of the traditional Chinese medicine composition is different, and the method is only one-step reflux extraction after ultrasound, specifically: mixing radix astragali with rhizoma bletilla, radix Gentianae, medulla Junci, galla chinensis and flos Lonicerae, and mixing with ethanol solution containing citric acid and acetic acid (citric acid: acetic acid=1:1, volume ratio) with pH of 5.3, wherein the solid-liquid ratio of the Chinese medicinal materials and ethanol solution is controlled to be 1:12g/mL, and the volume concentration of ethanol solution is 75%; after mixing, carrying out ultrasonic treatment with the frequency of 80kHz for 35min; and finally, carrying out reflux extraction for 2 times, wherein the temperature of each extraction is 65 ℃, the time of each extraction is 45min, filtering after each reflux extraction, combining the extracting solutions of each extraction, then regulating the pH to 7 by using 0.5% sodium hydroxide, concentrating under reduced pressure to remove the organic solvent, concentrating/drying at low temperature, and obtaining the traditional Chinese medicine composition.
Comparative example 7
Unlike example 1, the preparation method of the traditional Chinese medicine composition is different, specifically, the step (3) is different:
(1) - (2) as in example 1;
(3) Mixing the decoction dregs with radix Gentianae, galla chinensis and flos Lonicerae, pulverizing into 100 mesh powder, mixing with ethyl acetate solution, controlling solid-to-liquid ratio of the Chinese medicinal materials and ethanol solution to 1:12g/mL, and controlling volume concentration of ethyl acetate solution to 75%; after mixing, carrying out ultrasonic treatment with the frequency of 50kHz for 10min; finally, carrying out reflux extraction for 1 time at 50 ℃ for 90min, filtering, and concentrating under reduced pressure to remove organic solvent to obtain extract and medicinal herb residue;
(4) Mixing the astragalus extract in the step (1), the decoction mixed solution in the step (2) and the extract in the step (3), concentrating and drying at low temperature to obtain the traditional Chinese medicine composition.
Comparative test 1 bacteriostatic Effect
The preparation of the traditional Chinese medicine composition comprises the following steps: the dried Chinese medicinal compositions of the examples and comparative examples were mixed with sterilized ultrapure water to obtain a Chinese medicinal composition (sterile) having a concentration of 25. Mu.g.mL -1.
The escherichia coli, staphylococcus aureus and pseudomonas aeruginosa are respectively prepared into bacterial solutions with the concentration of 10 7 CFU/mL, 15mL of LB agar medium and 0.15mL of bacterial solution are added into a culture dish, and the bacterial solutions are quickly and evenly mixed. After the culture medium was solidified, oxford cups were placed uniformly in a petri dish, 100. Mu.L of each of the Chinese medicinal composition preparations to be tested of each of the examples and comparative examples of the present invention was added to each well, and ampicillin (25. Mu.g. ML -1) was used as a positive control, and 3 parallel groups were set for each group. The petri dish was placed in a constant temperature incubator at 37 ℃ for 24 hours for observation, and the diameter of the inhibition zone was measured with a vernier caliper. The specific method comprises the following steps: antibacterial effect judgment standard: the diameter r of the inhibition zone is extremely sensitive more than 20mm, the sensitivity is high when the diameter r is more than 15mm and less than 20mm, the sensitivity is moderate when the diameter r is more than 10mm and less than 15mm, and the sensitivity is low when the diameter r is less than 10 mm.
The diameter of a bacteriostasis ring of 25 mug/mL -1 ampicillin and a liquid to be tested on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa at different concentrations is measured by adopting an oxford cup method, and the detection result is shown in the following table 1:
TABLE 1
In table 1, P < 0.05, compared to example 1.
As can be seen from table 1, the traditional Chinese medicine composition provided by the invention has remarkable antibacterial activity, and the diameter of the inhibition zone is remarkably higher than that of the control group.
Comparative experiment 2 anti-inflammatory Effect
1. Mouse auricle swelling degree
72 Kunming Mice (KM) were randomly divided into 9 groups of 8, male and female halves each of 18-22g.
Mice were divided into matrix groups (negative control group): coating vaseline;
triamcinolone acetonide econazole cream group (positive control group);
the traditional Chinese medicine composition comprises the following components: the traditional Chinese medicine compositions of the embodiment and the comparative example of the invention are respectively smeared.
Vaseline (0.1 g/dose), triamcinolone acetonide econazole cream (0.1 g/dose) and a group of Chinese medicinal compositions (0.05 g/dose) are respectively smeared on the right auricle of each group of mice, and the mice are fed 2 times a day and fed in a single cage for 7 days. The preparation method comprises the following steps of fasted for 12 hours before the last administration, smearing 20 mu L of dimethylbenzene on the right ear of a mouse for 45 minutes after the administration, taking left and right ear pieces of the same part by using a puncher with the diameter of 6mm after 20 minutes, weighing by using a ten-thousandth balance, and taking the difference of the mass of the two ear pieces as auricle swelling degree.
Swelling inhibition = (negative control swelling degree-ear swelling degree)/negative control swelling degree
The ear swelling degree and swelling inhibition rate results of the mice are shown in Table 2:
TABLE 2
| Ear swelling degree mg | Swelling inhibition ratio (%) | |
| Negative control group (0.1 g/piece) | 5.79±0.56 | - |
| Positive control group (0.1 g/piece) | 3.80±0.55 | 34.41 |
| Example 1 (0.05 g/min) | 1.52±0.41** | 73.80 |
| Comparative example 1 (0.05 g/min) | 3.45±0.43 | 40.49 |
| Comparative example 2 (0.05 g/d) | 3.02±0.38* | 47.85 |
| Comparative example 3 (0.05 g/d) | 3.97±0.33 | 31.53 |
| Comparative example 4 (0.05 g/d) | 2.60±0.42* | 55.10 |
| Comparative example 5 (0.05 g/min) | 2.89±0.34* | 50.03 |
| Comparative example 6 (0.05 g/min) | 2.44±0.30* | 57.63 |
In table 2, P < 0.05 compared to the negative control group.
From the table above, it can be seen that the traditional Chinese medicine composition has a remarkable inhibiting effect on mice ear swelling caused by dimethylbenzene, and the anti-inflammatory effect is remarkably superior to that of a comparative example and a positive control group, and has statistical significance.
2. Anti-inflammatory effect of bacterial vaginitis in rats
And (3) model preparation: after 104 SD rats are fasted and not water-inhibited for 16 hours, chloral hydrate is anesthetized, 8 rats are separated into a sham operation group (only the ovaries are not removed by operation), the ovaries on both sides of the rats are removed under the aseptic condition of the rest rats, 10mg/kg -1 of estradiol benzoate is injected subcutaneously on the 4 th day after operation, 1 time a day for 4 days continuously, and the rats enter into the sham estrus. Namely, the injection is injected into the rats except for the estradiol benzoate, and then the injection is injected into the hydrocortisone injection 60 mg.kg -1, 1 time a day, 3 days after a day, and the model of the hypoimmunity of the rats is replicated.
On the 9 th day after operation, 0.1mL (0.25X10 6. ML -1) gardnerella liquid is injected into the vagina of the rat, and stays in situ for 1-2min, so as to prevent bacterial liquid from overflowing. The same procedure was repeated 1 more time after 24 h. The rats were alternately subcutaneously injected with estradiol benzoate or hydrocortisone injection 1 time every other day after infection. After 4d injection of gardnerella, the vulva red swelling of the rat model is seen, a small amount of white secretion is accompanied, the secretion is smeared on the surface of a blood agar culture medium, the culture is carried out for 72 hours at 37 ℃, and the bubble-like colonies with different sizes are observed visually, namely the bacterial vaginitis rat model is successfully copied.
The bacterial vaginitis model rats were randomly divided into a model (equal volume physiological saline), a compound metronidazole vaginal suppository (positive control 216mg kg -1) and high, medium and low doses (600, 250, 125mg kg -1) of the traditional Chinese medicine composition prepared in example 1, a medium dose (300 mg kg -1) of example 2 and comparative examples 1-6, and 8 of each group. The vaginal administration was carried out 1 time per day for 7 days. The sham group was given an equal volume of physiological saline. On day 7 after the last treatment, rats were anesthetized and 1mL of rat vaginal rinse was counted microscopically.
The abdominal aorta was sampled and the collected blood samples were centrifuged at 4℃for 25min (2500 r. Min -1) to prepare serum, and the TNF-. Alpha.content was measured.
The number of bacteria and the TNF- α content in serum are shown in Table 3:
TABLE 3 Table 3
| Dosage (mg kg -1) | Bacterial count (. Times.10 6) | TNF-α(U·mL-1) | |
| False operation group | - | 1.35±0.15## | 476.18±50.33## |
| Model group | - | 6.10±0.47** | 614.93±41.29** |
| Positive control group | 216 | 1.82±0.31## | 497.82±30.62## |
| Example 1 Low dose group | 125 | 1.88±0.26## | 459.83±34.50## |
| Dose group in example 1 | 250 | 1.49±0.27## | 427.17±21.66## |
| Example 1 high dose group | 600 | 1.45±0.30## | 415.30±39.83## |
| Example 2 | 250 | 1.56±0.28## | 437.11±20.25## |
| Comparative example 1 | 250 | 3.83±0.38*# | 516.70±32.08*# |
| Comparative example 2 | 250 | 4.02±0.48*# | 509.60±30.30*# |
| Comparative example 3 | 250 | 4.47±0.76*# | 549.85±27.07* |
| Comparative example 4 | 250 | 3.06±0.50*# | 493.77±21.39# |
| Comparative example 5 | 250 | 2.89±0.43*# | 487.03±38.50# |
| Comparative example 6 | 250 | 2.72±0.26*# | 473.30±35.98# |
In table 3, P < 0.05, < P < 0.05 compared to the dose group in example 1; compared with the model group, the # is less than 0.05, and the # P is less than 0.05.
As can be seen from Table 3, the bacterial count of the model group was significantly higher than that of the sham operation group, the bacterial count of the medium dose group of examples 1-2 and the high dose group of example 1 was significantly lower than that of the model group, and significantly lower than that of the comparative example group; compared with the model group, the traditional Chinese medicine composition can obviously reduce the level of the tumor necrosis factor TNF-alpha in the rat serum, and the effect is obviously better than the anti-inflammatory effect of the traditional Chinese medicine composition of the comparative example.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention, and that the simple modification and equivalent substitution of the technical solution of the present invention can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present invention.
Claims (11)
1. An anti-inflammatory and antibacterial traditional Chinese medicine composition is characterized by comprising the following components: astragalus root, bletilla tuber, gentian root, rush, phellodendron bark, chinese gall and honeysuckle flower.
2. The traditional Chinese medicine composition according to claim 1, which is characterized by comprising the following components in parts by weight: 10-16 parts of astragalus, 3-10 parts of bletilla, 3-8 parts of gentian, 1.5-6 parts of rush, 8-18 parts of phellodendron, 5-15 parts of gallnut and 3-8 parts of honeysuckle.
3. The traditional Chinese medicine composition according to claim 2, which is characterized by comprising the following components in parts by weight: 11-15 parts of astragalus, 4-7 parts of bletilla, 4-7 parts of gentian, 2-5 parts of rush, 10-15 parts of phellodendron, 6-10 parts of gallnut and 4-6 parts of honeysuckle.
4. The traditional Chinese medicine composition according to claim 3, which is characterized by comprising the following components in parts by weight: 13 parts of astragalus, 6 parts of bletilla, 5 parts of gentian, 3 parts of rush, 12 parts of phellodendron, 8 parts of gallnut and 5 parts of honeysuckle.
5. The traditional Chinese medicine composition according to claim 1, wherein the weight ratio of astragalus, gentian, gallnut and honeysuckle is 10-16:3-8:5-15:3-8; preferably, the weight ratio of the astragalus, the gentian, the gallnut and the honeysuckle is 11-15:4-7:6-10:4-6; further preferably, the weight ratio of the astragalus, the gentian, the gallnut and the honeysuckle is 13:5:8:5.
6. The method for preparing the traditional Chinese medicine composition according to any one of claims 1 to 5, comprising the steps of:
(1) Pulverizing radix astragali, mixing with alkaline organic solvent, and reflux extracting to obtain radix astragali extractive solution and radix astragali residue;
(2) Mixing the radix astragali residue with rhizoma Bletillae, medulla Junci, and cortex Phellodendri, soaking in water, and decocting to obtain decoction mixture and water decoction residue;
(3) Mixing the decoction dregs with gentian, chinese gall and honeysuckle, crushing, mixing with a weak acid organic solvent solution, then carrying out ultrasonic treatment, and finally carrying out reflux extraction to obtain an extracting solution extracted by the acid organic solvent solution and medicinal herb dregs;
(4) Mixing the astragalus extract in the step (1), the decoction mixed solution in the step (2) and the extract extracted by the acidic organic solvent solution in the step (3), and concentrating or drying to obtain the traditional Chinese medicine composition.
7. The method according to claim 6, wherein in the step (1), the particle size of the pulverized powder is 80 to 200 mesh; the organic solvent is at least one of ethanol, methanol, butanol and diethyl ether, the volume concentration of the organic solvent is 65-95%, and the solid-liquid ratio of astragalus root to the organic solvent is 1:8-16g/mL; the alkali in the alkaline organic solvent is at least one selected from sodium carbonate, sodium bicarbonate, food-grade sodium hydroxide and food-grade potassium hydroxide, and the pH is adjusted to 8.5-10.0; the times of reflux extraction are 2-4 times, the temperature of each extraction is 55-80 ℃, the extraction time is 30-120min, filtering, mixing the extracting solutions of each extraction, adjusting pH to neutrality, concentrating under reduced pressure, and removing organic solvent to obtain radix astragali extracting solution and radix astragali residue.
8. The method according to claim 6, wherein in the step (2), the water is added for soaking for 1-5 hours, and the solid-liquid ratio is 1:15-25g/mL; the times of the decoction are 1-3 times, each time of the decoction is 0.5-2 hours, the solid-liquid ratio of each time of the decoction is the same, the decoction is filtered, and the decoction is combined to obtain a decoction mixed solution and water decoction dregs.
9. The method according to claim 6, wherein in the step (3), the pulverized particles have a particle size of 50 to 100 mesh;
The organic solvent in the weak acid organic solvent solution is at least one selected from ethanol, acetone, methanol and ethyl acetate, the acid is at least one selected from citric acid, acetic acid, malic acid, tartaric acid and phosphoric acid, and the pH value of the weak acid organic solvent solution is adjusted to 4.5-6.8; the volume concentration of the organic solvent solution is 65-85%, and the solid-liquid ratio of the traditional Chinese medicine to the organic solvent solution is 1:8-16g/mL;
the ultrasonic treatment is carried out for 20-50min, and the ultrasonic frequency is 60-100kHz;
The times of reflux extraction are 2-3 times, the temperature of each extraction is 55-80 ℃, the extraction time is 30-90min, the extraction solutions of each extraction are filtered, the extraction solutions of each extraction are combined, and the organic solvent is removed by decompression concentration, so as to obtain the extraction solution extracted by the acid organic solvent solution and medicinal herb residues.
10. The application of the traditional Chinese medicine composition in preparing a preparation with anti-inflammatory and antibacterial effects is characterized in that the traditional Chinese medicine composition is prepared by the traditional Chinese medicine composition according to any one of claims 1-5 or the preparation method according to any one of claims 6-9.
11. A preparation with anti-inflammatory and antibacterial effects is characterized by comprising a traditional Chinese medicine composition and medical acceptable auxiliary materials; the traditional Chinese medicine composition is prepared by the traditional Chinese medicine composition according to any one of claims 1 to 5 or the preparation method according to any one of claims 6 to 9.
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