CN117598065B - Method for promoting quick germination of curculigo seeds - Google Patents

Method for promoting quick germination of curculigo seeds Download PDF

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CN117598065B
CN117598065B CN202410078201.7A CN202410078201A CN117598065B CN 117598065 B CN117598065 B CN 117598065B CN 202410078201 A CN202410078201 A CN 202410078201A CN 117598065 B CN117598065 B CN 117598065B
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seed
curculigo
seeds
spraying
soaking liquid
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CN117598065A (en
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杨美权
郭乔仪
张金渝
曾祥飞
起明菊
杨天梅
左应梅
杨维泽
许宗亮
王丽
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Chuxiong Technician College
Yunnan Plant Insect Medicine Biotechnology Co ltd
Institute of Medicinal Plants Yunnan Academy of Agricultural Sciences
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Chuxiong Technician College
Yunnan Plant Insect Medicine Biotechnology Co ltd
Institute of Medicinal Plants Yunnan Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
    • A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
    • A01N39/04—Aryloxy-acetic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
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  • Botany (AREA)
  • Inorganic Chemistry (AREA)
  • Soil Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention relates to the technical field of Chinese herbal medicine planting, and particularly discloses a method for promoting quick germination of curculigo seed, which comprises the following steps: selecting mature curculigo seeds; placing curculigo seed in seed soaking liquid A, wherein the volume ratio of the seed to the seed soaking liquid A is 1:5, standing and soaking for 40-45 min, and the seed soaking liquid A is 85-88% sulfuric acid water solution; then placing the seeds into seed soaking liquid B, wherein the volume ratio of the seeds to the seed soaking liquid B is 1-2:3, and shaking the seeds for 36-48 h on a shaking table at the frequency of 30-40 r/min, and the seed soaking liquid B is GA 3 100-150 mg/L, CPPU-8 mg/L and 2, 4-D100-120 mg/L, and the invention improves the curculigo seed by the technical proposalThe seed emergence rate shortens the seed emergence period.

Description

Method for promoting quick germination of curculigo seeds
Technical Field
The invention relates to the technical field of Chinese herbal medicine planting, in particular to a method for quickly germinating Chinese herbal medicine curculigo seed.
Background
Curculigo (Cruculigo orchioides Gaertn.) is a plant of Curculigo of Amaryllidaceae, and is used as a drug with rhizome, has effects of invigorating kidney yang, strengthening tendons and bones, dispelling dampness and cold, and is mainly used for treating symptoms such as impotence and cold sperm, flaccidity of tendons and bones, cold pain of waist and knees, yang deficiency and diarrhea, and with increase of market demand of Curculigo medicinal materials, wild resources of Curculigo is collected year by year, so that wild resources of Curculigo are increasingly reduced, and simultaneously, because wild Curculigo Mao Yiban grows under forest, damage to root systems of forest and water and soil loss may be caused when wild resources are collected and dug, so that ecological environment is destroyed. In recent years, artificial planting has gradually become trend and initially forms scale, a tissue culture mode is mainly adopted at present to quickly obtain the curculigo rhizome Mao Miaochong in a large scale, but compared with a seedling (seedling growing out by sowing seeds), the tissue culture seedling has the defects of low environmental tolerance, easiness in being influenced by diseases and insect pests in the later culture process because of a sterile environment, and high seedling raising period and serious limitation of high-quality seedling culture process because of unpredictable complex gene mixing of the seedling in the new variety seedling culture process, the seedling culture is still a main technical means in practice, generally the curculigo rhizome Mao Huaqi is 6-10 months, the seed collection date is 8-11 months, one fruit pod contains 10-15 seeds, 3-5 fruit pods can be collected in one year, the curculigo rhizome seeds have the defects of low seedling emergence rate, long bud emergence time and the like, when the curculigo rhizome is used for seedling culture, the seedling raising period is long, and the seedling raising cost is high.
Disclosure of Invention
Aiming at the defects of low seedling emergence rate, long seedling emergence time and the like of curculigo rhizome seeds in the seed seedling raising process in the prior art, the invention particularly provides a method for promoting the rapid germination of curculigo rhizome seeds so as to improve the seedling emergence rate of the curculigo rhizome seeds, shorten the seedling emergence period of the seeds, lay a foundation for cultivating new variety seedlings and reduce the cultivation cost.
The invention is realized by the following scheme:
a method for promoting rapid germination of curculigo seeds, comprising the steps of:
(1) Selecting mature curculigo seeds, removing pericarps, cleaning and drying in the shade for later use;
(2) Placing curculigo seed in seed soaking liquid A, wherein the volume ratio of the seed to the seed soaking liquid A is 1:5, standing and soaking for 40-45 min, and stirring once every 5min during the soaking, wherein the seed soaking liquid A is 85-88% sulfuric acid water solution;
(3) Washing the curculigo seed treated in the step (2) with clear water for 5-6 times, placing the curculigo seed in seed soaking liquid B, placing the curculigo seed and seed soaking liquid B on a shaking table in a volume ratio of 1-2:3, and applying frequency of 30-40 r/minShaking for 36-48 h, wherein the seed soaking liquid B is GA 3 100-150 mg/L, CPPU-8 mg/L and 2, 4-D100-120 mg/L;
(4) Uniformly mixing the seeds treated in the step (3) with fine soil according to the mass ratio of 1:10, and then broadcasting the mixture on the soil surface, and covering the fine soil with the thickness of 1-2 cm;
(5) Spraying water by an atomization nozzle after sowing, and covering a mulching film for moisturizing;
(6) After 10 days of sowing, spraying nutrient solution by an atomization spray nozzle for uncovering the film, coating the film after spraying, and spraying once every 15-20 days, wherein the spraying amount is 100-150 kg/mu each time, and spraying for 6-8 times.
Further, in the step (3), when the seed soaking liquid B is used, 3 to 5 drops of Tween 80 are added dropwise per liter to serve as a surfactant.
Further, the nutrient solution in the step (6) is GA 3 400-500 mg/L, CPPU-40 mg/L and 1.0-1.2 g/L of ammonium nitrate.
The invention achieves the effects of
Generally, seeds which are difficult to emerge are treated by adopting a single method such as warm water seed soaking, hormone seed soaking, low-temperature lamination, friction, concentrated sulfuric acid and the like, and the seed emergence rate of curculigo rhizome seeds cannot be obviously improved by adopting the methods such as warm water seed soaking, low-temperature lamination and the like through experiments, the seedling emergence period cannot be shortened, and the seed emergence rate or the seedling emergence period can be improved to a certain extent when the seeds are treated by adopting the concentrated sulfuric acid, part of the seed soaking, friction and the like, wherein the seed emergence period can be shortened from 8 months to about 6 months without treatment by adopting the concentrated sulfuric acid and the friction treatment, and the seed emergence rate can be improved to more than 10.3% from about 0.5% without treatment by adopting the friction treatment, so that the seed coat possibly has a layer of hard substances which prevent water absorption. However, in the actual operation process, when concentrated sulfuric acid is used for treatment, the seeds are usually burnt and dead when the time is too long, so that the emergence period of the seeds is shortened, but the emergence rate is reduced due to the fact that part of the seeds are burnt and dead, but the effect of passivating the seed coats cannot be achieved due to too short time, and the friction treatment is extremely easy to take labor, and meanwhile, the operation difficulty is high and the data repetition stability is poor. Therefore, on the basis of concentrated sulfuric acid basic test data, a large number of tests are carried out by reducing the sulfuric acid concentration, controlling the seed soaking time, controlling the volume ratio of seeds to sulfuric acid and the like, and the invention discovers that after 85-88% sulfuric acid solution and seeds are uniformly mixed according to the proportion of 5:1, standing and soaking for 40-45 min, the seed emergence rate can be improved to more than 55%, and the seed emergence time is shortened to about 5 months. Then soaking seeds treated by 85-88% sulfuric acid in mixed solution of GA3, NAA and 2,4-D with a certain concentration, and then spraying mixed solution of GA3, NAA and ammonium nitrate with a certain concentration on a soil surface at regular intervals, so that the emergence rate of seeds can be improved to more than 80%, and the emergence period can be shortened to less than 4 months. And the data reproducibility is good, the operation is simple and convenient, and the time and the labor are saved.
Drawings
FIG. 1 is a graph showing the effect of 8 months after sowing seeds in different treatment modes; a is the treatment of comparative example 1; b is the treatment of example 1; c is the treatment of example 2;
FIG. 2 example 1 shows a plot of seed emergence 4 months after sowing;
FIG. 3 is a plot of the emergence of seeds 4 months after sowing in comparative example 1.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. Plant growth regulator GA used in the present invention 3 (gibberellin), CPPU (forchlorfenuron) and 2,4-D (2, 4-dichlorophenoxyacetic acid) are commercially available.
Example 1
1. Harvesting mature curculigo rhizome fruits, cleaning soil on the surface of the curculigo rhizome fruits with clear water, peeling seeds, cleaning the curculigo rhizome fruits with clear water, and airing the curculigo rhizome fruits on a dry gauze in a ventilated and shady place for later use;
2. the curculigo seeds after being dried in the shade are placed in 85% sulfuric acid water solution (seed soaking solution A), the volume ratio of the seeds to the 85% sulfuric acid water solution is 1:5, the curculigo seeds are placed still for soaking for 45min, and the curculigo seeds are stirred for 1 time with a glass rod every 5min during the soaking.
3. Taking out seeds immersed in 85% sulfuric acid water solution, washing with clear water for 6 times to remove sulfuric acid, and dripping GA with 5 drops of Tween 80 3 In seed soaking liquid B with the concentration of 100mg/L, CPPU mg/L and 2,4-D of 100mg/L, the volume ratio of the seeds to the seed soaking liquid B is 1:3, then placing the mixture on a shaking table, and shaking the mixture for 48 hours at the frequency of 30 r/min.
4. The seeds treated by the steps are uniformly mixed with fine soil in a mass ratio of 1:10 after being drained, and then are sowed on the soil surface, and the fine soil with the mass ratio of 1-2 cm is covered.
5. After sowing, water is sprayed by an atomization spray nozzle and a mulching film is coated for moisturizing.
6. After 10 days of sowing, spraying GA by an atomization nozzle for uncovering the film 3 400mg/L, CPPU mg/L and 1.0g/L of ammonium nitrate, and then coating the film after spraying, and spraying once every 15 days, wherein the spraying amount is 100 kg/mu, and the total spraying is 8 times.
Example 2
1. Harvesting mature curculigo rhizome fruits, cleaning soil on the surface of the curculigo rhizome fruits with clear water, peeling seeds, cleaning the curculigo rhizome fruits with clear water, and airing the curculigo rhizome fruits on a dry gauze in a ventilated and shady place for later use;
2. the curculigo seeds after being dried in the shade are placed in 88% sulfuric acid water solution (seed soaking solution A), the volume ratio of the seeds to the 88% sulfuric acid water solution is 1:5, the curculigo seeds are placed still for soaking for 40min, and the curculigo seeds are stirred for 1 time with a glass rod every 5min during the soaking.
3. Taking out the seeds immersed in 88% sulfuric acid water solution, washing the seeds with clear water for 6 times to remove sulfuric acid, and then placing the seeds in a seed immersing solution B in which 5 drops of GA3 150mg/L, CPPU mg/L and 2, 4-D120 mg/L of Tween 80 are added dropwise, wherein the volume ratio of the seeds to the seed immersing solution B is 2:3, then placing the mixture on a shaking table, and shaking the mixture for 36 hours at the frequency of 40 r/min.
4. The seeds treated by the steps are uniformly mixed with fine soil in a mass ratio of 1:10 after being drained, and then are sowed on the soil surface, and the fine soil with the mass ratio of 1-2 cm is covered.
5. After sowing, water is sprayed by an atomization spray nozzle and a mulching film is coated for moisturizing.
6. After 10 days of sowing, spraying mixed nutrient solution of GA3 500mg/L, CPPU mg/L and ammonium nitrate 1.2g/L by using an atomization spray nozzle, coating the film after spraying, and spraying once every 20 days, wherein the spraying amount is 150 kg/mu each time, and spraying 6 times.
Comparative example 1
1. Harvesting mature curculigo rhizome fruits, cleaning soil on the surface of the curculigo rhizome fruits with clear water, peeling seeds, cleaning the curculigo rhizome fruits with clear water, and airing the curculigo rhizome fruits on a dry gauze in a ventilated and shady place for later use;
2. uniformly mixing seeds and fine soil according to the mass ratio of 1:10, and then sowing the mixture on the soil surface, and covering the fine soil with the thickness of 1-2 cm.
3. After sowing, water is sprayed by an atomization spray nozzle, and a mulching film is covered for moisturizing.
4. After 10 days of sowing, spraying clear water by an atomization spray head, coating the film after spraying, and spraying once every 15 days, wherein the spraying amount is 100 kg/mu each time.
Comparative example 2
1. Harvesting mature curculigo rhizome fruits, cleaning soil on the surface of the curculigo rhizome fruits with clear water, peeling seeds, cleaning the curculigo rhizome fruits with clear water, and airing the curculigo rhizome fruits on a dry gauze in a ventilated and shady place for later use;
2. placing the curculigo seed after drying in the shade in 85% sulfuric acid water solution, wherein the volume ratio of the seed to the 85% sulfuric acid water solution is 1:5, standing and soaking the seed for 45min, and stirring the seed with a glass rod for 1 time every 5min during soaking.
3. The seeds treated by 85% sulfuric acid aqueous solution are washed by clear water for 6 times, drained, evenly mixed with fine soil according to the mass ratio of 1:10, and then sowed on the soil surface, and the fine soil with the mass ratio of 1-2 cm is covered.
5. After sowing, water is sprayed by an atomization spray nozzle, and a mulching film is covered for moisturizing.
6. After 10 days of sowing, spraying clear water by an atomization spray head, coating the film after spraying, and spraying once every 15 days, wherein the spraying amount is 100 kg/mu each time.
Comparative example 3
1. Harvesting mature curculigo rhizome fruits, cleaning soil on the surface of the curculigo rhizome fruits with clear water, peeling seeds, cleaning the curculigo rhizome fruits with clear water, and airing the curculigo rhizome fruits on a dry gauze in a ventilated and shady place for later use;
2. placing the curculigo seed after drying in the shade in 85% sulfuric acid water solution, wherein the volume ratio of the seed to the 85% sulfuric acid water solution is 1:5, standing and soaking the seed for 45min, and stirring the seed with a glass rod for 1 time every 5min during soaking.
3. Taking out seeds immersed in 85% sulfuric acid water solution, washing with clear water for 6 times to remove sulfuric acid, and dripping GA with 5 drops of Tween 80 3 In seed soaking liquid B with the concentration of 100mg/L, CPPU mg/L and 2,4-D of 100mg/L, the volume ratio of the seeds to the seed soaking liquid B is 1:3, then placing the mixture on a shaking table, and shaking the mixture for 48 hours at the frequency of 30 r/min.
4. The seeds treated by the steps are uniformly mixed with fine soil in a mass ratio of 1:10 after being drained, and then are sowed on the soil surface, and the fine soil with the mass ratio of 1-2 cm is covered.
5. After sowing, water is sprayed by an atomization spray nozzle, and a mulching film is covered for moisturizing.
6. After 10 days of sowing, spraying clear water by an atomization spray head, coating the film after spraying, and spraying once every 15 days, wherein the spraying amount is 100 kg/mu each time.
Comparative example 4
1. Harvesting mature curculigo rhizome fruits, cleaning soil on the surface of the curculigo rhizome fruits with clear water, peeling seeds, cleaning the curculigo rhizome fruits with clear water, and airing the curculigo rhizome fruits on a dry gauze in a ventilated and shady place for later use;
2. placing curculigo seed after drying in the shade in seed soaking liquid B with GA3 100mg/L, CPPU 5mg/L and 2, 4-D100 mg/L of Tween 80 dropwise, wherein the volume ratio of the seed to the seed soaking liquid B is 1:3, then placing the mixture on a shaking table, and shaking the mixture for 36 hours at the frequency of 30 r/min.
3. The seeds soaked by the seeds are uniformly mixed with fine soil according to the mass ratio of 1:10 after being drained, and then are sowed on the soil surface, and the fine soil with the mass ratio of 1-2 cm is covered.
5. After sowing, water is sprayed by an atomization spray nozzle, and a mulching film is covered for moisturizing.
6. After 10 days of sowing, spraying mixed nutrient solution of GA3 400mg/L, CPPU mg/L and ammonium nitrate 1.0g/L by using an atomization spray nozzle, coating the film after spraying, and spraying once every 15 days, wherein the spraying amount is 100 kg/mu, and the total spraying is 8 times.
Comparative example 5
1. Harvesting mature curculigo rhizome fruits, cleaning soil on the surface of the curculigo rhizome fruits with clear water, peeling seeds, cleaning the curculigo rhizome fruits with clear water, and airing the curculigo rhizome fruits on a dry gauze in a ventilated and shady place for later use;
2. placing the curculigo seed after drying in the shade in GA with 5 drops of Tween 80 3 In seed soaking liquid B with the concentration of 100mg/L, CPPU mg/L and 2,4-D of 100mg/L, the volume ratio of the seeds to the seed soaking liquid B is 1:3, then placing the mixture on a shaking table, and shaking the mixture for 36 hours at the frequency of 30 r/min.
3. And (3) airing the seeds subjected to seed soaking, placing the seeds in 85% sulfuric acid aqueous solution after surface moisture is dried, wherein the volume ratio of the seeds to the 85% sulfuric acid aqueous solution is 1:5, standing and soaking the seeds for 40-45 min, and stirring the seeds with a glass rod for 1 time every 5min during seed soaking.
4. The seeds after the sulfuric acid treatment are washed by clear water for 6 times, drained, evenly mixed with fine soil according to the mass ratio of 1:10, and then sowed on the soil surface, and the fine soil with the mass ratio of 1-2 cm is covered.
5. After sowing, water is sprayed by an atomization spray nozzle, and a mulching film is covered for moisturizing.
6. After 10 days of sowing, spraying GA by an atomization nozzle for uncovering the film 3 400mg/L, CPPU mg/L and 1.0g/L of ammonium nitrate, and then coating the film after spraying, and spraying once every 15 days, wherein the spraying amount is 100 kg/mu, and the total spraying is 8 times.
The seed emergence times and emergence rates after treatment using examples 1 and 2 and comparative examples 1 to 5 are shown in table 1 below, while referring to fig. 1 to 3, the emergence times are shortened by about 2 months and the emergence rates reach 84.67% compared with comparative examples 1 to 5 in the treatment mode of the present invention, and the seedling time and seedling cost are remarkably reduced.
TABLE 1 Effect of different treatments on Curculigo seed
Treatment of First seed emergence time Seed emergence rate of 4 months (120 d) Seed emergence rate of 6 months (180 d) Seed emergence rate of 8 months (240 d)
Example 1 For 86 days 84.67% 87.04% 87.12%
Example 2 81 days 82.35% 83.49% 85.10%
Comparative example 1 For 143 days 0 1.89% 4.73%
Comparative example 2 120 days 0.19% 41.65% 49.76%
ComparisonExample 3 97 days 33.90% 67.94% 70.34%
Comparative example 4 135 days 0 10.67% 24.55%
Comparative example 5 108 days 0.38% 51.60% 68.13%
Finally, it is noted that the above-mentioned embodiments are only intended to illustrate rather than limit the invention, and that, although the above-mentioned examples have been described in detail with reference to the method according to the invention, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (1)

1. A method for promoting quick germination of curculigo seeds, which is characterized by comprising the following steps:
(1) Selecting mature curculigo seeds, removing pericarps, cleaning and drying in the shade for later use;
(2) Placing curculigo seed in seed soaking liquid A, wherein the volume ratio of the seed to the seed soaking liquid A is 1:5, standing and soaking for 40-45 min, and stirring once every 5min during the soaking, wherein the seed soaking liquid A is 85-88% sulfuric acid water solution;
(3) Washing the curculigo seed treated in the step (2) with clear water for 5-6 times, and then placing the curculigo seed in seed soaking liquid B, wherein the volume ratio of the seed to the seed soaking liquid B is 1-to-over2:3 shaking for 36-48 h on a shaking table at the frequency of 30-40 r/min, wherein the seed soaking liquid B is GA 3 100-150 mg/L, CPPU-8 mg/L and 2, 4-D100-120 mg/L;
(4) Uniformly mixing the seeds treated in the step (3) with fine soil according to the mass ratio of 1:10, and then broadcasting the mixture on the soil surface, and covering the fine soil with the thickness of 1-2 cm;
(5) Spraying water by an atomization nozzle after sowing, and covering a mulching film for moisturizing;
(6) After 10 days of sowing, spraying nutrient solution by an atomization spray nozzle, coating the film after spraying, and spraying once every 15-20 days, wherein the spraying amount is 100-150 kg/mu each time, and spraying for 6-8 times;
step (3), when the seed soaking liquid B is used, 3-5 drops of Tween 80 are added dropwise per liter to serve as a surfactant;
the nutrient solution in the step (6) is GA 3 400-500 mg/L, CPPU-40 mg/L and 1.0-1.2 g/L of ammonium nitrate.
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