CN117567201B

CN117567201B - Method for improving domestication survival rate and growth rate of seedling of Xian Mao Zupei

Info

Publication number: CN117567201B
Application number: CN202410067084.4A
Authority: CN
Inventors: 杨天梅; 曾祥飞; 杨美权; 张金渝; 许宗亮; 杨维泽; 左应梅; 王丽
Original assignee: Yunnan Plant Insect Medicine Biotechnology Co ltd; Institute of Medicinal Plants Yunnan Academy of Agricultural Sciences
Current assignee: Yunnan Plant Insect Medicine Biotechnology Co ltd; Institute of Medicinal Plants Yunnan Academy of Agricultural Sciences
Priority date: 2024-01-17
Filing date: 2024-01-17
Publication date: 2024-03-15
Anticipated expiration: 2044-01-17
Also published as: CN117567201A

Abstract

The invention relates to the technical field of traditional Chinese medicine seedling raising, and particularly discloses a method for improving domestication survival rate and growth rate of a seedling of Xian Mao Zupei, wherein the nutrient solution is at least one of nutrient solution A, nutrient solution B or nutrient solution C, and the nutrient solution A comprises the following components: 25-40 mg/L methyl jasmonate and 20-30 mg/L naphthylacetic acid; the nutrient solution B comprises the following components: 80-120 mg/L methyl jasmonate and 40-60 mg/L naphthylacetic acid; the nutrient solution C comprises the following components: aiming at the defects of the prior art, the invention applies relevant nutrient solution in the domestication period, so that the survival rate of the Xian Mao Xunhua reaches more than 95 percent, the leaves are hardly dehydrated in the domestication process, and the domestication and seedling time is shortened.

Description

Method for improving domestication survival rate and growth rate of seedling of Xian Mao Zupei

Technical Field

The invention relates to the technical field of traditional Chinese medicine seedling raising, in particular to a method for improving domestication survival rate and growth rate of seedling of Xian Mao Zupei.

Background

Rhizoma Curculiginis (Cruculigo orchioides Gaertn.) is plant of Curculigo belonging to Amaryllidaceae, has effects of invigorating kidney yang, strengthening tendons and bones, and dispelling dampness and cold, and is mainly used for treating sexual impotence, cold sperm, flaccidity of tendons and bones, lumbago, knee pain, yang deficiency, diarrhea, etc.

Along with the increase of the market demand of curculigo medicinal materials, the wild resources of curculigo are collected and increased year by year, so that the wild resources of curculigo are increasingly reduced, and meanwhile, as wild curculigo Mao Yiban grows under the forest, the wild resources may cause the damage of the root system of the forest and the water and soil loss when being collected and dug, so that the ecological environment is damaged. In recent years, artificial planting has gradually become a trend and is initially scaled up.

Because the curculigo seed is difficult to collect, the seed germination rate is extremely low, the seed quantity is small, and the like, the seedling of the curculigo Mao Zupei becomes the immortal Mao Chongmiao to obtain the most important propagation mode, and the technical development and production of the seedling of the curculigo Mao Zupei are carried out in the early stage of the team, but the root rot and the leaf water stain and serious are found in the domestication and seedling hardening process of the seedling of the curculigo Mao Zupei, the survival rate can only reach about 40% at most, and the leaf is dehydrated within 1-2 days when the moisture of the small arch is not adopted, the survival rate can reach about 60%, but even if the surviving seedling of the curculigo Mao Miao is withered, the new leaf can be sent out in 20-30 days, the domestication period of the seedling of the curculigo Mao Zupei is prolonged for at least 3-4 months, the seedling raising cost is increased, and the large-scale curculigo is unfavorable for raising seedlings.

Disclosure of Invention

The invention aims to provide a method for improving the domestication survival rate and the growth rate of the seedling of Xian Mao Zupei, which solves the problems of high death rate and long growth period in the process of cultivating tissue culture seedlings.

In order to achieve the above purpose, the invention adopts the following technical scheme:

the invention provides a nutrient solution for improving domestication survival rate of seedling of Xian Mao Zupei, which is at least one of nutrient solution A, nutrient solution B or nutrient solution C,

the nutrient solution A comprises the following components: 25-40 mg/L methyl jasmonate and 20-30 mg/L naphthylacetic acid;

the nutrient solution B comprises the following components: 80-120 mg/L methyl jasmonate and 40-60 mg/L naphthylacetic acid;

the nutrient solution C comprises the following components: 80 to 120mg/L of naphthylacetic acid, 20 to 30mg/L of kinetin, 1.0 to 1.5g/L of monopotassium phosphate and 0.5 to 1.0g/L of alginic acid.

The invention also provides a method for improving the domestication survival rate of the seedling of the Xian Mao Zupei, which comprises the following steps:

(1) Cleaning a basal culture medium of the seedling of the Chinese character Xian Mao Zupei, and planting the culture medium in a mixed matrix, wherein the mixed matrix is formed by mixing coconut husk, humus soil and garden soil according to a mass ratio of 3:3:5;

(2) After planting, spraying nutrient solution A on the leaves according to the usage amount of 30-40 kg per mu, and building a small arch shed for moisturizing after spraying;

(3) After 10-15 days of planting, spraying the nutrient solution B on the leaves according to the usage amount of 30-40 kg per mu;

(4) After planting for 25-35 days, spraying a nutrient solution C on the leaves according to the usage amount of 30-40 kg per mu;

(5) At 45 days after planting, the small arch shed is completely uncovered, and 0.1% of water-soluble compound fertilizer is poured and applied once every 15 days; the compound fertilizer N: p: k=15:15:20.

The beneficial technical effects achieved by the invention are as follows:

1. according to the invention, the domestication matrix adopts the mixed matrix of coconut husk, humus soil and garden soil in a ratio of 3:3:5, so that on one hand, the contradiction between air and moisture in the matrix can be solved to the greatest extent, the base part has enough moisture supply during domestication of the seedling Mao Zupei, meanwhile, oxygen deficiency caused by excessive moisture can be avoided, and the occurrence of root rot in the domestication process of the seedling Mao Zupei can be reduced to a certain extent.

2. In the domestication process of the seedling of the curculigo orchioides Mao Zupei, nutrient solution A and nutrient solution B are sprayed in the initial stage of domestication, nutrient solution C with a certain concentration is sprayed in the early stage of domestication, the domestication survival rate of the seedling of the curculigo orchioides Mao Zupei can be improved to more than 95%, and the mechanism is hypothesized that the methyl jasmonate can effectively induce the closing of pores of the seedling of the curculigo orchioides Mao Shepian in the initial stage, so that the transpiration effect of leaves is reduced, the stress reaction of the seedling of the curculigo orchioides Mao Zupei to drought stress is induced, and the naphthylacetic acid can promote the growth of the root system of the curculigo orchioides, so that the root system absorption capacity is increased, the growth of the seedling of the curculigo orchioides is regulated, the absorption capacity of the nutrient components of the curculigo orchioides is enhanced, and then potassium dihydrogen phosphate with a certain concentration and alginic acid are matched, so that the seedling of the curculigo orchioides Mao Miao can grow fast, and the seedling is more robust, and the seedling of the stress resistance to hypoxia, water shortage, root rot and the seedling is enhanced.

3. According to the invention, the corresponding nutrient solution is sprayed on the leaf surfaces within 30 days before domestication of the curculigo orchioides, so that the problem of leaf withering is avoided, the occurrence of root rot is reduced, the curculigo orchioides Mao Xunsu is promoted to be converted from heterotrophic into autotrophic and nutrient is accumulated, so that the formation of fleshy roots at the base of the stems is accelerated, and the sprayed nutrient solution has certain nitrogen, phosphorus and potassium, so that the curculigo orchioides Mao Zupei seedlings can be promoted to expand and gradually form fleshy roots within 2 months, and compared with the control of being sprayed by only assisting the same chemical fertilizer but not adopting the nutrient solution disclosed by the invention, the fleshy roots are promoted to be fast for 2-3 months.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention.

Fig. 1: root rot pattern of the seedling of xian Mao Zupei in comparative example 1;

fig. 2: building a small arch shed and then preserving a moisturizing field map;

fig. 3: example 1 a 2 month old acclimatization survival effect graph of tissue culture seedlings;

fig. 4: a graph of the survival effect of 2 months old domestication of the seedling of the Xian Mao Zupei in comparative example 2;

fig. 5: in the embodiment 1, the expansion effect of the 2 month old fleshy root domesticated by the tissue culture seedling is shown;

fig. 6: the effect of domesticating 6 month-old fleshy root expansion of the tissue culture seedling in the example 1 is shown (commercial seedling is already adopted);

fig. 7: overall effect of acclimatizing the tissue culture seedlings to 6 month old seedlings in example 2.

Detailed Description

The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Example 1

The tissue culture seedling adopts the invention with the patent number: ZL201810969410.5; the name is: the tissue culture seedling produced in example 1 of the method for in vitro culture and plant regeneration of the Xian Mao Shepian is subjected to domestication and seedling hardening, and the method comprises the following steps:

(1) Cleaning a basal culture medium of the seedling of the immortal Mao Zupei, and planting the seedling in a mixed matrix of coconut husk, humus soil and garden soil in a mass ratio of 3:3:5.

(2) After planting, spraying nutrient solution A consisting of 25mg/L methyl jasmonate and 20mg/L naphthylacetic acid on leaf surfaces, wherein the spraying amount is 30 kg/mu, and after spraying, building a small arch shed for moisturizing.

(3) After 10 days of planting, uncovering the small arch shed, spraying nutrient solution B consisting of 80mg/L of methyl jasmonate and 40mg/L of naphthylacetic acid on leaf surfaces, wherein the spraying amount is 35kg, covering the small arch shed after the spraying is finished, and opening two ends to start reducing the air humidity.

(4) After planting for 25 days, uncovering the small arch shed, spraying a nutrient solution C consisting of 80mg/L of naphthalene acetic acid, 20mg/L of kinetin, 1.0g/L of monopotassium phosphate and 0.5g/L of alginic acid on leaf surfaces, wherein the spraying amount is 40kg, covering the small arch shed after spraying, and opening two ends and uncovering two sides to 30cm high.

(5) At 45 days after planting, the small arch shed is completely uncovered, and 0.1% water-soluble fertilizer (N: P: K=15:15:20) is applied at intervals of 15 days.

(6) And respectively measuring survival rate, seedling height and stem basal fleshy root expansion rate on 60 days, 120 days and 180 days after planting.

Example 2

(2) After planting, spraying a nutrient solution A consisting of 30mg/L methyl jasmonate and 30mg/L naphthylacetic acid on leaf surfaces, wherein the spraying amount is 30 kg/mu, and after spraying, building a small arch shed for moisturizing.

(3) After 15 days of planting, uncovering the small arch shed, spraying nutrient solution B consisting of 100mg/L of methyl jasmonate and 60mg/L of naphthylacetic acid on leaf surfaces, wherein the spraying amount is 35kg, covering the small arch shed after the spraying is finished, and opening two ends to start reducing the air humidity.

(4) After 35 days of planting, the small arch shed is uncovered, and the foliage is sprayed with nutrient solution C consisting of 100mg/L of naphthalene acetic acid, 25mg/L of kinetin, 1.5g/L of monopotassium phosphate and 0.8g/L of alginic acid, wherein the spraying amount is 35kg, and after spraying, the small arch shed is covered, but both ends are opened and both sides are uncovered to 30cm high.

Example 3

(2) After planting, spraying nutrient solution A consisting of 40mg/L methyl jasmonate and 25mg/L naphthylacetic acid on leaf surfaces, wherein the spraying amount is 40 kg/mu, and after spraying, building a small arch shed for moisturizing.

(3) After 10 days of planting, the small arch shed is uncovered, the foliage is sprayed with nutrient solution B consisting of 120mg/L of methyl jasmonate and 50mg/L of naphthylacetic acid, the spraying amount is 40kg, after the spraying is finished, the small arch shed is covered, and two ends are opened to start reducing the air humidity.

(4) After planting for 30 days, uncovering the small arch shed, spraying a nutrient solution C consisting of 120mg/L of naphthylacetic acid, 30mg/L of kinetin, 1.0g/L of monopotassium phosphate and 1.0g/L of alginic acid on leaf surfaces, wherein the spraying amount is 40kg, covering the small arch shed after spraying, and opening two ends and uncovering two sides to 30cm high.

Comparative example 1

The tissue culture seedling adopts the invention with the patent number: ZL201810969410.5, name: the tissue culture seedling produced in the example 1 in the method for the in vitro culture and plant regeneration of the Xian Mao Shepian is subjected to domestication and seedling hardening, and the steps comprise:

(1) The basal culture medium of the seedling of the immortal Mao Zupei is cleaned and planted in the field soil.

(2) After planting, a small arch shed is built, the humidity of a matrix is kept at about 60%, the air humidity is kept at more than 90% in the early stage, and the air humidity is gradually reduced to be the same as that of a large environment in the later stage.

(3) Opening two ends of the small arch shed after 10 days, and completely uncovering the small arch shed after 25 days until the two sides of the small arch shed reach 30cm in height and 45 days.

(4) 45 days after planting, 0.1% water-soluble fertilizer (N: P: K=15:15:20) is applied at 30kg per mu, and then applied once every 15 days.

(5) And respectively measuring survival rate, seedling height and stem basal fleshy root expansion rate on 60 days, 120 days and 180 days after planting.

Comparative example 2

(2) After planting, the humidity of the matrix is kept at about 60 percent.

(3) 45 days after planting, 0.1% water-soluble fertilizer (N: P: K=15:15:20) is applied at 30kg per mu, and then applied once every 15 days.

(4) And respectively measuring survival rate, seedling height and stem basal fleshy root expansion rate on 60 days, 120 days and 180 days after planting.

Comparative example 3

(1) Cleaning a basal culture medium of the seedling of the immortal Mao Zupei, and planting the seedling in a mixed matrix of coconut husk, humus soil and garden soil in a ratio of 3:3:5.

Comparative example 4

Comparative example 5

(3) 10 days after planting, uncovering the small arch shed, spraying nutrient solution B consisting of 80mg/L of methyl jasmonate and 40mg/L of naphthylacetic acid on leaf surfaces, wherein the spraying amount is 30kg, covering the small arch shed after spraying is finished, and opening two ends to start reducing air humidity.

(4) And when the seedlings are planted, the two sides of the small arch shed are uncovered to 30cm high, and when the seedlings are planted for 45 days, the small arch shed is completely uncovered.

In the above examples and comparative examples, the five-point sampling method was used for measurement and calculation, and the survival rate=the number of survival seedlings counted in the counting time/the total number of seedlings in the counting area×100%; then, from the respective treatment areas, the average seedling height of Curculigo orchioides in the statistical interval at the statistical time, the fleshy root expansion ratio=the number of fleshy root expanded seedlings of the statistical time/the total number of seedlings in the statistical area×100%, respectively.

Table 1 statistics of different examples and comparative examples

	Survival rate of 60d	Rate of expansion of 60d fleshy root	Expansion rate of 120 d-old fleshy root	180Expansion rate of d-year fleshy root	180 d-old seedling height
						Example 1	97.4%	34.6%	99.7%	100%	31.6cm
Example 2	96.9%	52.7%	100%	100%	32.8cm
						Example 3	97.3%	46.8%	100%	100%	29.1cm
Comparative example 1	37.8%	10.3%	56.4%	90.2%	24.7cm
						Comparative example 2	61.9%	0	21.7%	84.6%	16.3cm
Comparative example 3	71.4%	8.9%	52.2%	91.8%	30.4cm
						Comparative example 4	89.3%	18.4%	71.6%	100%	31.8cm
Comparative example 5	92.6%	21.7%	90.4%	100%	30.9cm

Note that: the expansion rate of the fleshy root at each month age described in the above table is statistical data of the surviving seedlings, the surviving seedlings are not counted in, and the 6-month (180 d) age seedling height is an average height in the statistical range.

Referring to the field condition diagrams of the seedlings of the Xian Mao Zupei under the treatment of examples and comparative examples, as can be seen by combining with table 1, the survival rate and the expansion speed of the fleshy roots of the examples 1, 2 and 3 are obviously higher than those of the comparative example 1 which is not subjected to other operations only by building a small arch shed and the comparative example 2 which is not subjected to other operations only by building a small arch shed, and when the mixed matrix of the coconut husk, the humus soil and the garden soil of the invention of 3:3:5 is adopted as the domesticated matrix, the survival rate and the expansion speed of the fleshy roots are slightly improved compared with those of the comparative example 2, but the ideal results cannot be achieved, while the survival rate and the expansion speed of the fleshy roots of the comparative example 4 are further improved by spraying the methyl jasmonate and the naphthylacetic acid on the basis of the comparative example 3, and the survival rate and the growth speed of the comparative example 5 are further improved by further spraying the methyl jasmonate and the naphthylacetic acid on the basis of the comparative example 4 after 10 days.

Claims

1. The method for improving the domestication survival rate of the seedling of the Xian Mao Zupei is characterized by comprising the following steps:

(1) Cleaning a basal culture medium of the seedling of the Chinese character Xian Mao Zupei, and planting the culture medium in a mixed matrix, wherein the mixed matrix is formed by mixing coconut coir, humus soil and garden soil according to a mass ratio of 3:3:5;

(5) At 45 days after planting, the small arch shed is completely uncovered, and 0.1% of water-soluble compound fertilizer is poured and applied once every 15 days; the compound fertilizer N: p: k=15:15:20;