CN117511749A - 一种棘孢木霉HG1与枯草芽孢杆菌Tpb55的固态发酵方法及其应用 - Google Patents
一种棘孢木霉HG1与枯草芽孢杆菌Tpb55的固态发酵方法及其应用 Download PDFInfo
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- CN117511749A CN117511749A CN202311422245.9A CN202311422245A CN117511749A CN 117511749 A CN117511749 A CN 117511749A CN 202311422245 A CN202311422245 A CN 202311422245A CN 117511749 A CN117511749 A CN 117511749A
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000010563 solid-state fermentation Methods 0.000 title claims abstract description 24
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- 239000001963 growth medium Substances 0.000 claims abstract description 37
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- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 51
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
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- Pest Control & Pesticides (AREA)
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- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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Abstract
Description
接种比例 | 相对抑菌率(%) |
t | 24.59±0.99c |
h | 44.36±0.52ab |
1:1 | 43.54±1.13ab |
1:2 | 40.92±1.60b |
2:1 | 46.17±0.55a |
1:10 | 44.15±1.46ab |
10:1 | 41.14±1.55b |
接种时间/h | 枯草芽孢杆(×109CFU/g) | 棘孢木霉(×1010CFU/g) |
t | 193.50±0.18a | - |
h | - | 1.49±0.05b |
th | 9.72±0.18c | 1.13±0.04bc |
th12 | 15.80±0.58b | 6.75±0.45a |
th24 | 7.88±0.23d | 1.08±0.09bc |
th36 | 2.88±0.90e | 0.86±0.05bcd |
ht24 | 2.34±0.18e | 1.49±0.59b |
ht36 | 2.75±0.40e | 0.33±0.03cd |
ht48 | 1.53±0.09e | 0.20±0.02d |
组别 | 相对抑菌率(%) | 组别 | 相对抑菌率(%) |
h1 | 14.81±0.71g | th243 | 6.58±0.41i |
h2 | 62.55±0.41c | th361 | 7.41±0.00i |
h3 | 11.94±1.09h | th362 | 67.90±0.71b |
t1 | 51.03±0.41e | th363 | 3.70±0.71j |
t2 | 7.41±0.71i | ht241 | 4.52±0.82j |
t3 | 11.53±0.82h | ht242 | 52.27±1.65e |
th1 | 12.35±0.71h | ht243 | 6.99±0.82i |
th2 | 63.37±0.41c | ht361 | 17.69±0.41f |
th3 | 6.99±0.82i | ht362 | 63.79±0.41c |
th121 | 7.82±0.41i | ht363 | 6.58±0.41i |
th122 | 73.25±0.41a | ht481 | 18.52±0.71f |
th123 | 7.41±0.71i | ht482 | 55.56±0.71d |
th241 | 10.29±1.09h | ht483 | 4.11±0.41j |
th242 | 68.73±0.41b | - | - |
Claims (10)
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CN202311422245.9A CN117511749B (zh) | 2023-10-31 | 一种棘孢木霉HG1与枯草芽孢杆菌Tpb55的固态发酵方法及其应用 |
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CN202311422245.9A CN117511749B (zh) | 2023-10-31 | 一种棘孢木霉HG1与枯草芽孢杆菌Tpb55的固态发酵方法及其应用 |
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Publication Number | Publication Date |
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CN117511749A true CN117511749A (zh) | 2024-02-06 |
CN117511749B CN117511749B (zh) | 2024-07-09 |
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