CN117427024A - Probiotics mouthwash for pets and preparation method and use method thereof - Google Patents
Probiotics mouthwash for pets and preparation method and use method thereof Download PDFInfo
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- CN117427024A CN117427024A CN202311504476.4A CN202311504476A CN117427024A CN 117427024 A CN117427024 A CN 117427024A CN 202311504476 A CN202311504476 A CN 202311504476A CN 117427024 A CN117427024 A CN 117427024A
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- mouthwash
- pets
- probiotic
- seed solution
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- 239000002324 mouth wash Substances 0.000 title claims abstract description 105
- 229940051866 mouthwash Drugs 0.000 title claims abstract description 99
- 239000006041 probiotic Substances 0.000 title claims abstract description 80
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000000529 probiotic effect Effects 0.000 claims abstract description 68
- 239000000243 solution Substances 0.000 claims abstract description 54
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- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
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- 229940029339 inulin Drugs 0.000 description 2
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- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 1
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- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940078795 lactoferrin Drugs 0.000 description 1
- 235000021242 lactoferrin Nutrition 0.000 description 1
- 229940069445 licorice extract Drugs 0.000 description 1
- 210000002200 mouth mucosa Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
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- 239000000419 plant extract Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- MCSINKKTEDDPNK-UHFFFAOYSA-N propyl propionate Chemical compound CCCOC(=O)CC MCSINKKTEDDPNK-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 229940010747 sodium hyaluronate Drugs 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Fodder In General (AREA)
Abstract
The embodiment of the disclosure discloses a probiotic mouthwash for pets, and a preparation method and a use method thereof. The preparation method of the probiotic mouthwash for pets comprises the following steps: step 1: mixing seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, molasses and purified water under stirring, and fermenting until pH value of the mixed solution is 3.0-5.5 to obtain mother solution; step 2: mixing the mother solution with Mycobacterium phlei seed solution, photosynthetic bacteria seed solution, molasses and purified water under stirring, and fermenting until the pH value of the mixed solution is 3.0-6.0 to obtain the probiotic collutory for pets. The preparation method of the probiotic mouthwash for pets, provided by the embodiment of the disclosure, prepares the probiotic mouthwash for pets by mixing and fermenting a plurality of bacterial groups, and has the advantages of simple steps, easily obtained raw materials, low requirement on environment, no chemical addition, no environmental pollution and the like.
Description
Technical Field
The disclosure relates to the technical field of pet mouthwashes, in particular to a probiotic mouthwash for pets, and a preparation method and a use method thereof.
Background
In the pet raising process, peculiar smell is often generated, and is mainly formed by mixing body odor and bad breath. 99% of body malodour can be rapidly resolved by a thorough whole body clean up, while bad breath is a long lasting battle involving digestive system conditioning and daily oral care throughout the life. Oral inflammation caused by bacteria, viruses, allergy or immunity such as dental calculus, gingivitis and stomatitis can cause bad breath, especially excessive growth of bacteria and inflammation attack, and can cause bad breath, and the bad breath needs to be treated in time. In addition to disease causes, the daily eating of more tasty foods, such as fish, liver foods, etc., can cause bad breath. Clinically, about 60% of pet cats over one year old have different degrees of oral problems, and about 90% of pet cats over four years old have different degrees of dental calculus. Oral problems such as dental calculus and gingivitis easily induce health problems in pet cats, and prevent the pet cats from approaching and interacting with the pet owners. About 80% of pet dogs over three years old have problems of bad breath, dental plaque, dental calculus, gingivitis, periodontal disease and the like, and even a few of the pet dogs die due to secondary infection caused by oral infection clinically, and many of the pet dogs lose normal diet capacity due to dental caries and dental caries.
The inventor finds that the mouthwash on the market is obtained by mixing and stirring chemical components, the chemical components are complex, the manufacturing process is complex, and the chemical components have uncontrollable influence on the health of pets, so that the mouthwash is not suitable for long-term use.
Disclosure of Invention
In view of the above, the embodiments of the present disclosure provide a preparation method of a probiotic mouthwash for pets and a probiotic mouthwash for pets, which at least partially solve the problems of complex chemical components and complex manufacturing process.
In a first aspect, an embodiment of the present disclosure provides a method for preparing a probiotic mouthwash for pets, comprising:
step 1: mixing seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, molasses and purified water under stirring, and fermenting until pH value of the mixed solution is 3.0-5.5 to obtain mother solution;
step 2: mixing the mother solution with Mycobacterium phlei seed solution, photosynthetic bacteria seed solution, molasses and purified water under stirring, and fermenting until the pH value of the mixed solution is 3.0-6.0 to obtain the probiotic collutory for pets.
Optionally, the proportion of each component in the step 1 is as follows: 1 to 2 parts of seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic saccharomycetes, 0.5 to 2 parts of molasses and 6 to 8.5 parts of purified water.
Optionally, the proportion of each component in the step 2 is as follows: 10 parts of mother solution, 0.5-2 parts of mycobacterium phlei seed solution, 0.5-2 parts of photosynthetic bacteria seed solution, 1-5 parts of molasses and 50-100 parts of purified water.
Optionally, fermenting the mixed liquor in the step 1 in an anaerobic environment;
and (3) fermenting the mixed liquor in the step (2) in an aerobic environment.
Optionally, in the step 1, the mother liquor is obtained when the pH value of the mixed liquor is 3.0.
Optionally, in the step 2, the probiotic mouthwash for pets is obtained when the pH value of the mixed solution is 3.0.
Optionally, the concentration of the pet-derived lactobacillus salivarius in the seed solution containing the pet-derived lactobacillus salivarius, the bacillus licheniformis and the anaerobic yeast is 3.7X10 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL；
The concentration of Mycobacterium phlei in the Mycobacterium phlei seed solution is 4.6X10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
In a second aspect, an embodiment of the present disclosure provides a probiotic mouthwash for pets, which is prepared by the preparation method of the probiotic mouthwash for pets.
In a third aspect, embodiments of the present disclosure provide a method of using a probiotic mouthwash for pets, comprising:
the probiotic mouthwash for pets and the drinking water for pets are mixed according to the mass ratio of 1:10-1:100, and the mixture is drunk by the pets.
The preparation method of the probiotic mouthwash for pets, provided by the embodiment of the disclosure, prepares the probiotic mouthwash for pets by mixing and fermenting a plurality of bacterial groups, and has the advantages of simple steps, easily obtained raw materials, low requirement on environment, no chemical addition, no environmental pollution and the like.
The components of the probiotic mouthwash for pets comprise composite components including pet-derived lactobacillus salivarius, bacillus licheniformis, anaerobic saccharomycetes, mycobacterium phlegmatis, photosynthetic bacteria and biological enzymes, bacteriocins, cellulase, protease and the like which are generated in the middle of the propagation process in the fermentation process, so that food residues remained in the oral cavity of the pets can be degraded, peculiar smell generated by fermentation of the food residues is reduced, and the generation of dental calculus is reduced. Because the mouthwash is obtained by combining a plurality of microbial agents, the mouthwash has stronger and more stable growth promoting capability than a single microbial agent.
The metabolites of Mycobacterium phlei and photosynthetic bacteria can be used as food sources for fermentation processes of Lactobacillus and Bacillus licheniformis, and can help the bacteria form a more stable state.
Through the synergistic effect among the probiotics strains, the growth of harmful bacteria in the oral cavity is inhibited, the ecological balance of the flora in the oral cavity of the pet is regulated, and the health of the oral cavity is maintained. In the production process of the probiotic mouthwash for pets, metabolites produced by probiotics can effectively prevent dental plaque and reduce tooth decay.
Through repeated experiments of the inventor, the concentration and the proportion of the probiotics provided by the embodiment of the disclosure can lead to richer flora and higher stability of fermentation liquor.
On the other hand, the mouthwash provided by the embodiment of the disclosure is produced by fermentation of pure natural probiotics, does not add any antibiotics, essence and preservative, and is safer than other types of mouthwashes. The foregoing description is only an overview of the disclosed technology, and may be implemented in accordance with the disclosure of the present disclosure, so that the above-mentioned and other objects, features and advantages of the present disclosure can be more clearly understood, and the following detailed description of the preferred embodiments is given with reference to the accompanying drawings.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present disclosure, and other drawings may be obtained according to these drawings without inventive effort to a person of ordinary skill in the art.
Fig. 1 is a flowchart of a method for preparing a probiotic mouthwash for pets according to an embodiment of the present disclosure;
fig. 2 is a schematic diagram of a preparation method of a probiotic mouthwash for pets according to an embodiment of the present disclosure.
Detailed Description
The present disclosure is described in further detail below with reference to the drawings and the embodiments. It is to be understood that the specific embodiments described herein are merely illustrative of the relevant content and not limiting of the present disclosure. It should be further noted that, for convenience of description, only a portion relevant to the present disclosure is shown in the drawings.
In addition, embodiments of the present disclosure and features of the embodiments may be combined with each other without conflict. The technical aspects of the present disclosure will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
Unless otherwise indicated, the exemplary implementations/embodiments shown are to be understood as providing exemplary features of various details of some ways in which the technical concepts of the present disclosure may be practiced. Thus, unless otherwise indicated, features of the various implementations/embodiments may be additionally combined, separated, interchanged, and/or rearranged without departing from the technical concepts of the present disclosure.
The use of cross-hatching and/or shading in the drawings is typically used to clarify the boundaries between adjacent components. As such, the presence or absence of cross-hatching or shading does not convey or represent any preference or requirement for a particular material, material property, dimension, proportion, commonality between illustrated components, and/or any other characteristic, attribute, property, etc. of a component, unless indicated. In addition, in the drawings, the size and relative sizes of elements may be exaggerated for clarity and/or descriptive purposes. While the exemplary embodiments may be variously implemented, the specific process sequences may be performed in a different order than that described. For example, two consecutively described processes may be performed substantially simultaneously or in reverse order from that described. Moreover, like reference numerals designate like parts.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. Furthermore, when the terms "comprises" and/or "comprising," and variations thereof, are used in the present specification, the presence of stated features, integers, steps, operations, elements, components, and/or groups thereof is described, but the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof is not precluded. It is also noted that, as used herein, the terms "substantially," "about," and other similar terms are used as approximation terms and not as degree terms, and as such, are used to explain the inherent deviations of measured, calculated, and/or provided values that would be recognized by one of ordinary skill in the art.
In the pet raising process, peculiar smell is often generated, and is mainly formed by mixing body odor and bad breath. 99% of body malodour can be rapidly resolved by a thorough whole body clean up, while bad breath is a long lasting battle involving digestive system conditioning and daily oral care throughout the life. Oral inflammation caused by bacteria, viruses, allergy or immunity such as dental calculus, gingivitis and stomatitis can cause bad breath, especially excessive growth of bacteria and inflammation attack, and can cause bad breath, and the bad breath needs to be treated in time. In addition to disease causes, the daily eating of more tasty foods, such as fish, liver foods, etc., can cause bad breath. Clinically, about 60% of pet cats over one year old have different degrees of oral problems, and about 90% of pet cats over four years old have different degrees of dental calculus. Oral problems such as dental calculus and gingivitis easily induce health problems in pet cats, and prevent the pet cats from approaching and interacting with the pet owners. About 80% of pet dogs over three years old have problems of bad breath, dental plaque, dental calculus, gingivitis, periodontal disease and the like, and even a few of the pet dogs die due to secondary infection caused by oral infection clinically, and many of the pet dogs lose normal diet capacity due to dental caries and dental caries.
Three approaches are generally available for preventing the problem of bad breath of pets, namely, paying attention to gastrointestinal health, and promoting digestion and improving the gastrointestinal environment by using probiotics, inulin and the like; secondly, the teeth are brushed frequently, and gauze is used for cleaning or a toothbrush is used for brushing teeth. Thirdly, selecting tooth cleaning water or mouthwash, and adding the tooth cleaning water or the mouthwash into daily drinking water.
At present, the method for regulating gastrointestinal health by adopting probiotics, inulin and the like has long period and insignificant effect for reducing the bad breath of pets. When brushing teeth, the slightly hard bristles of the toothbrush can cause a part of pets to be sensitive and escape, and two people are often required to operate together, which is too troublesome. The tooth cleaning water or the tooth cleaning water is friendly to pets and has high acceptance, but the ingredients of the tooth cleaning water for pets in the market are disordered, and the safety is not guaranteed. At present, the chlorhexidine compounds are mainly CHX (chlorhexidine acetate and chlorhexidine gluconate) and CPC (cetylpyridinium chloride). Chlorhexidine is also a main raw material of the gynecological inflammation cleaner, and is a cationic surfactant. Chlorhexidine is used to prevent intra-oral infections in immunocompromised animals and to treat gingivitis and periodontal disease. The safe dose of chlorhexidine keeps the pet's teeth healthy, but is not used for a long period of time. There are clear studies showing that prolonged use of CHX can lead to staining of the teeth and mucosal damage in pets and the appearance of oral diseases. In addition, CHX odors also make some pets reluctant to drink water. CPC is an oral antibacterial substance frequently applied in human medicine, and experiments prove that the CPC can control bad breath of pets and prevent periodontal diseases. However, the side effects of CPC used for a long time or at a high concentration are similar to those of CHX, such as staining of tooth surfaces, damage of oral mucosa, and increase of the probability of canker sore. In recent years, plant extracts such as tea leaf extract, persimmon extract and the like have been used for cleaning the oral cavity of pets, and although they are nontoxic and harmless, they are weak in efficacy relative to CHX and CPC and do not remove the oral malodour of pets well. In addition, the mouthwash which adopts antibacterial substances to reduce the bad breath of the pet is mainly used for inhibiting the propagation of bacteria in the mouth of the pet. However, the mouthwash has no obvious effect of cleaning the oral cavity, and improper use can cause disturbance of oral flora of pets, severe conditions and oral diseases such as dental ulcer.
The inventor finds that the mouthwash on the market is obtained by mixing and stirring chemical components, the chemical components are complex, the manufacturing process is complex, and the chemical components have uncontrollable influence on the health of pets, so that the mouthwash is not suitable for long-term use.
In order to solve the above problems, referring to fig. 1 and 2, the embodiment of the present specification provides a method for preparing a probiotic mouthwash for pets.
Sulfur dioxide and hydrogen sulfide are two components contained in the oral malodor of pets, and are mainly produced by fermentation of oral food residues. Subsequent examples demonstrate that mouthwashes can achieve malodor reduction by verifying that mouthwashes can reduce sulfur dioxide and hydrogen sulfide.
Example 1
A method for preparing probiotic mouthwash for pets, comprising the following steps:
step 1: fully stirring and mixing seed solution 1L containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, molasses 0.5L and purified water 8.5L, fermenting in a sealed plastic barrel until pH value of the mixed solution is 3.0 to obtain 10L mother liquor, wherein the concentration of the pet-derived lactobacillus salivarius is 3.7X10 in the seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
Step 2: mixing mother liquor 10L with Mycobacterium phlei seed solution 0.5L, photosynthetic bacteria seed solution 0.5L, molasses 1L, and purified water 50L, stirring thoroughly, fermenting in plastic barrel with air holes until pH of the mixed solution is 3.5, and packaging the fermentation product aseptically to obtain probiotic collutory for pet, wherein Mycobacterium phlei seed solution has a concentration of 4.6X10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
Experiments were conducted on the mouthwash obtained in this example, and the experimental results are as follows:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The results showed that the removal rate of hydrogen sulfide was 92.2%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide of the mouthwash is 87.8% within 24 hours.
Example 2
A method for preparing probiotic mouthwash for pets, comprising the following steps:
step 1: fully stirring and mixing seed solution 2L containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic saccharomycetes, molasses 2L and purified water 6L, putting into a sealed plastic bucket for fermentation until the pH value of the mixed solution is 5.5, and preparing 10L mother liquor. In the seed solution containing the pet-derived Lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, the concentration of the pet-derived Lactobacillus salivarius is 3.7X10 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
Step 2: mixing mother liquor 10L with Mycobacterium phlei seed solution 2L, photosynthetic bacteria seed solution 2L, molasses 5L, and purified water 50L, stirring thoroughly, fermenting in plastic barrel with air holesWhen the pH value of the mixed solution is 6.0, aseptic packaging is carried out on the fermentation product to obtain the probiotic mouthwash for pets, wherein the concentration of the mycobacterium phlei in the mycobacterium phlei seed solution is 4.6x10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
Experiments were conducted on the mouthwash obtained in this example, and the experimental results are as follows:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The results showed that the removal rate of hydrogen sulfide was 88.6%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide of the mouthwash is 85.5% within 24 hours.
Example 3
A method for preparing probiotic mouthwash for pets, comprising the following steps:
step 1: 1.5L of seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic saccharomycetes, 1L of molasses and 7.5L of purified water are fully stirred and mixed, and put into a sealed plastic bucket for fermentation until the pH value of the mixed solution is 4.0, so as to prepare 10L of mother solution. In the seed solution containing the pet-derived Lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, the concentration of the pet-derived Lactobacillus salivarius is 3.7X10 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
Step 2: taking 10L of mother liquor, fully stirring and mixing with 1L of mycobacterium phlegmatis seed liquor, 1L of photosynthetic bacteria seed liquor, 3L of molasses and 60L of purified water, putting into a plastic barrel with air holes for fermentation, and carrying out aseptic packaging on the fermentation product when the pH value of the fermentation mixture is 5.0 to obtain the probiotic mouthwash for pets, wherein the concentration of the mycobacterium phlegmatis in the mycobacterium phlegmatis seed liquor is 4.6×10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
Experiments were conducted on the mouthwash obtained in this example, and the experimental results are as follows:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The results showed that the removal rate of hydrogen sulfide was 89.3%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide of the mouthwash is 87.1% within 24 hours.
Example 4
A method for preparing probiotic mouthwash for pets, comprising the following steps:
step 1: fully stirring and mixing seed solution 1L containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, molasses 2L and purified water 7L, fermenting in a sealed plastic barrel until pH of the mixed solution is 5.5 to obtain 10L mother liquor, wherein the concentration of the pet-derived lactobacillus salivarius is 3.7X10 in the seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
Step 2: taking 10L of mother liquor, fully stirring and mixing with 2L of mycobacterium phlegmatis seed liquor, 2L of photosynthetic bacteria seed liquor, 5L of molasses and 100L of purified water, putting into a plastic barrel with air holes for fermentation, and carrying out aseptic packaging on the fermentation product when the pH value of the fermentation mixture is 5.0 to obtain the probiotic mouthwash for pets, wherein the concentration of the mycobacterium phlegmatis in the mycobacterium phlegmatis seed liquor is 4.6X10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
Experiments were conducted on the mouthwash obtained in this example, and the experimental results are as follows:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The results showed that the removal rate of hydrogen sulfide was 87.5%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide of the mouthwash is 79.6% within 24 hours.
Example 5
A method for preparing probiotic mouthwash for pets, comprising the following steps:
step 1: fully stirring and mixing 2L of seed liquid containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic saccharomycetes, 0.5L of molasses and 7.5L of purified water, putting into a sealed plastic bucket for fermentation until the pH value of the mixed liquid is 5.5, and preparing 10L of mother liquid. In the seed solution containing the pet-derived Lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, the concentration of the pet-derived Lactobacillus salivarius is 3.7X10 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
Step 2: mixing mother liquor 10L with Mycobacterium phlei seed solution 2L, photosynthetic bacteria seed solution 0.5L, molasses 3L, and purified water 75L, stirring thoroughly, fermenting in plastic barrel with air holes until pH of the mixed solution is 6.0, and packaging under aseptic condition to obtain probiotic collutory for pet, wherein Mycobacterium phlei seed solution has a concentration of 4.6X10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
Experiments were conducted on the mouthwash obtained in this example, and the experimental results are as follows:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The results showed that the removal rate of hydrogen sulfide was 86.8%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide of the mouthwash is 88.3% within 24 hours.
Example 6
A method for preparing probiotic mouthwash for pets, comprising the following steps:
step 1: mixing seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast 1.5L, molasses 1.5L and purified water 7L, stirring thoroughly, fermenting in a sealed plastic barrel until pH value of the mixed solution is 5.5, and preparing 10L mother liquor. In the seed solution containing the pet-derived Lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, the concentration of the pet-derived Lactobacillus salivarius is 3.7X10 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
Step 2: taking 10L of mother liquor, fully stirring and mixing with 1.5L of mycobacterium phlegmatis seed liquor, 1.5L of photosynthetic bacteria seed liquor, 4L of molasses and 80L of purified water, putting into a plastic barrel with air holes for fermentation, and carrying out aseptic packaging on the fermentation product when the pH value of the fermentation mixture is 4.0 to obtain the probiotic mouthwash for pets, wherein the concentration of mycobacterium phlegmatis in the mycobacterium phlegmatis seed liquor is 4.6X10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
Experiments were conducted on the mouthwash obtained in this example, and the experimental results are as follows:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The result showed that the removal rate of hydrogen sulfide was 91.3%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide of the mouthwash is 86.6% within 24 hours.
Example 7
A method for preparing probiotic mouthwash for pets, comprising the following steps:
step 1: 1.8L of seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic saccharomycetes, 1.2L of molasses and 7L of purified water are fully stirred and mixed, put into a sealed plastic bucket for fermentation for 6 days, and 10L of mother solution is prepared when the pH value of the mixed solution is 4.5. In the seed solution containing the pet-derived Lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, the concentration of the pet-derived Lactobacillus salivarius is 3.7X10 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
Step 2: mixing mother liquor 10L with Mycobacterium phlei seed solution 1L, photosynthetic bacteria seed solution 2L, molasses 3L, and purified water 100L, stirring thoroughly, fermenting in plastic barrel with air holes until pH of the mixed solution is 4.0, and packaging the fermentation product under aseptic condition to obtain probiotic collutory for pets, wherein Mycobacterium phlei seed solution has concentration of 4.6X10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
Experiments were conducted on the mouthwash obtained in this example, and the experimental results are as follows:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The results showed that the removal rate of hydrogen sulfide was 88.2%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide of the mouthwash is 85.8% within 24 hours.
Comparative example
(1) Adding the sour agent into distilled water, stirring and dissolving, adding glycerol, and stirring uniformly to obtain a clear and uniform mixed solution;
(2) Adding essence into the mixed solution, and uniformly mixing;
(3) Adding fructo-oligosaccharide, preservative, lactobacillus acidophilus, lactoferrin, licorice extract, persimmon fruit extract and sodium hyaluronate into the mixed solution in the step (2), stirring and mixing after each component is added until the components are completely dissolved, and starting the addition of the next component until all the components are uniformly mixed;
(4) And (3) standing the mixed solution obtained in the step (3), and filtering to obtain the general tooth cleaning water for dogs and cats.
The mouthwash obtained in the comparative example was subjected to the following experiment:
15L of hydrogen sulfide gas was circulated at a flow rate of 1L/min through a large bubble absorber tube containing 10mL of probiotic mouthwash for pets using the CJ/T516-2017 assay method for 24h. And collecting the processed gas and analyzing the concentration of the gas. The results showed that the removal rate of hydrogen sulfide was 20.1%.
100mL of the mouthwash is evenly sprayed on 3 pieces of 1m for three times by adopting a QB/T2761-2006 detection method 2 Naturally airing the base paper, and then placing the base paper into a position of 1.5m 3 And detecting in the test cabin. The results show that the removal rate of sulfur dioxide in the mouthwash is 25.2% within 24 hours.
The embodiment of the disclosure provides a probiotic mouthwash for pets, which is prepared by the preparation method of the probiotic mouthwash for pets. The viable count of lactic acid bacteria in the probiotic mouthwash for pets is more than or equal to 8.7X10 4 cfu/mL, the viable count of bacillus is more than or equal to 3.5X10 5 cfu/mL。
In a third aspect, embodiments of the present disclosure provide a method of using a probiotic mouthwash for pets, comprising:
the probiotic mouthwash for pets and the drinking water for pets are mixed according to the mass ratio of 1:10-1:100, and the mixture is drunk by the pets.
The probiotic mouthwash for pets is added into drinking water, and the effects of reducing the bad breath and protecting the oral health can be achieved through drinking water. The number of times of brushing the teeth of the pet can be reduced to 1 time/week from original 3-4 times/week.
The present disclosure has the following advantages:
(1) the probiotic mouthwash for pets is produced by fermenting pure natural probiotics, does not add any chemical preparations such as antibiotics, essence, preservative and the like, and is safer than other types of mouthwashes.
(2) The probiotic mouthwash for pets is convenient to use and has longer duration of cleaning the oral cavity. Only according to 1: the ratio of 10-100 is added into drinking water of the pet, the pet can maintain oral health for a long time through drinking water, and the tooth brushing frequency can be reduced to 1 time/week from original 3-4 times/week.
(3) The probiotics in the mouthwash can degrade food residues remained in the oral cavity of the pet, reduce peculiar smell generated by fermentation of the food residues and reduce the generation of dental calculus;
(4) through the synergistic effect among the probiotics strains, the growth of harmful bacteria in the oral cavity is inhibited, the ecological balance of the flora in the oral cavity of the pet is regulated, and the health of the oral cavity is maintained;
(5) in the production of probiotic mouthwashes for pets, metabolites produced by the probiotics, for example: biological enzymes, bacteriocins, cellulase, protease and the like can effectively prevent dental plaque, reduce tooth decay and play a good role in oral health care.
In the description of the present specification, reference to the terms "one embodiment/manner," "some embodiments/manner," "example," "specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment/manner or example is included in at least one embodiment/manner or example of the present application. In this specification, the schematic representations of the above terms are not necessarily for the same embodiment/manner or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments/modes or examples. Furthermore, the various embodiments/modes or examples described in this specification and the features of the various embodiments/modes or examples can be combined and combined by persons skilled in the art without contradiction.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "plurality" is at least two, such as two, three, etc., unless explicitly defined otherwise.
It will be appreciated by those skilled in the art that the above-described embodiments are merely for clarity of illustration of the disclosure, and are not intended to limit the scope of the disclosure. Other variations or modifications will be apparent to persons skilled in the art from the foregoing disclosure, and such variations or modifications are intended to be within the scope of the present disclosure.
Claims (10)
1. A method for preparing probiotic mouthwash for pets, which is characterized by comprising the following steps:
step 1: mixing seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic yeast, molasses and purified water under stirring, and fermenting until pH value of the mixed solution is 3.0-5.5 to obtain mother solution;
step 2: mixing the mother solution with Mycobacterium phlei seed solution, photosynthetic bacteria seed solution, molasses and purified water under stirring, and fermenting until the pH value of the mixed solution is 3.0-6.0 to obtain the probiotic collutory for pets.
2. The method for preparing the probiotic mouthwash for pets according to claim 1, wherein the proportion of each component in the step 1 is as follows: 1 to 2 parts of seed solution containing pet-derived lactobacillus salivarius, bacillus licheniformis and anaerobic saccharomycetes, 0.5 to 2 parts of molasses and 6 to 8.5 parts of purified water.
3. The method for preparing the probiotic mouthwash for pets according to claim 2, wherein the proportion of each component in the step 2 is as follows: 10 parts of mother solution, 0.5-2 parts of mycobacterium phlei seed solution, 0.5-2 parts of photosynthetic bacteria seed solution, 1-5 parts of molasses and 50-100 parts of purified water.
4. The method for preparing the probiotic mouthwash for pets according to claim 1, wherein the mixed liquor in the step 1 is fermented in an anaerobic environment; and (3) fermenting the mixed liquor in the step (2) in an aerobic environment.
5. The method for preparing a probiotic mouthwash for pets according to claim 4, wherein in the step 1, the mother liquor is obtained when the pH value of the mixed liquor is 3.0.
6. The method for preparing the probiotic mouthwash for pets according to claim 4, wherein in the step 2, the probiotic mouthwash for pets is obtained when the pH value of the mixed solution is 3.0.
7. The method for preparing a probiotic mouthwash for pets according to any of claims 1 to 6, wherein the concentration of the pet-derived lactobacillus salivarius in the seed solution containing the pet-derived lactobacillus salivarius, the bacillus licheniformis and the anaerobic yeast is 3.7x10 5 cfu/mL, concentration of Bacillus licheniformis is 3.5X10 5 cfu/mL, concentration of anaerobic yeast is 2.7X10 5 cfu/mL。
8. The method for preparing a probiotic mouthwash for pets according to any of claims 1 to 6, wherein the concentration of mycobacterium phlei in the mycobacterium phlei seed solution is 4.6x10 5 cfu/mL, the concentration of photosynthetic bacteria in the photosynthetic bacteria seed solution is 5.0X10 5 cfu/mL。
9. A probiotic mouthwash for pets, characterized in that the probiotic mouthwash for pets is prepared by the preparation method of the probiotic mouthwash for pets according to any of claims 1 to 8.
10. A method of using a probiotic mouthwash for pets, comprising:
the probiotic mouthwash for pets according to claim 9 is mixed with drinking water for pets according to a mass ratio of 1:10-1:100, and the mixture is drunk by pets.
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