CN1172593C - Method of pronoting plant growth and enhancing anti reverse property - Google Patents
Method of pronoting plant growth and enhancing anti reverse property Download PDFInfo
- Publication number
- CN1172593C CN1172593C CNB02138598XA CN02138598A CN1172593C CN 1172593 C CN1172593 C CN 1172593C CN B02138598X A CNB02138598X A CN B02138598XA CN 02138598 A CN02138598 A CN 02138598A CN 1172593 C CN1172593 C CN 1172593C
- Authority
- CN
- China
- Prior art keywords
- ala
- plant
- leaf
- blade
- seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a method for promoting the plant growth and enhancing the stress resistance, which belongs to the application field of biochemical reagents on agricultural production. 0.01 of 100 mg/L hydrochloric acid-5-amino levulinic acid or 50 to 500 mg/L of levulinic acid are used for continuously soaking seeds or watering root systems or spraying blades for 1 to 3 days with the frequency of once per day. The present invention has the advantages of high plant chlorophyll content, air vent open promotion, improved net photosynthetic rate, reduced photorespiration intensity, soluble sugar increase and dry matter accumulation increase, so the plant stress resistance is improved, and the yield is improved. Because the 5-amino levulinic acid per se is an endogenous metabolism product and has the advantages of biological degradation, no residue, no pollution and nontoxicity on human bodies, the method of the present invention can be used for field and installation agricultural nuisance free production. Musk melon seedlings (the left seedlings are treated seedlings and the right seedlings are reference seedlings) after low temperature (8 DEG C) treatment for 6 hours are shown in a picture.
Description
(1) technical field: a kind of plant growing and method that strengthens anti-low temperature and poor light ability of promoting of the present invention, belong to the application of biochemical reagents in agricultural production, be exclusively used in the plant photosynthesis ability that improves, promote growth and development of plants, increase output, strengthen anti-low temperature and poor light.
(2) technical background: growth and development of plants often is subjected to the restriction of various unsuitable environmental conditions, often causes growth and output to descend.Though can reach the purpose of increasing both production and income by the crop varieties that improves agricultural environment or seed selection strong stress resistance, its cost height, year limit for length also may bring other seondary effect.For example; since the last century the nineties; China's agricultural facility culture technique is more and more universal; though it has improved the component environment factor of plant growth; but side effects such as the low light level that is caused, secondary salinization also can't solve so far; and, reduce production capacity because its majority is that low temperature season carries out in the winter time, and the low temperature stress at night is the entail dangers to plant life usually.There have been some national inventing patents can be used to improve photosynthesis of plant, strengthened resistance.But, be mostly with several a large amount of or trace elements and (or) plant hormone cooperates by a certain percentage.Because the composition complexity, the mechanism of action is indeterminate, and using method is difficult for being grasped by the producer, and result of use is often also unstable.Also have some to research and propose materials such as using the plant hormone abscisic acid and improve plant cold resistance, but its cost is to suppress plant growing, reduces output.List of references CN1132592A " improves the method for plant salt endurance " and has proposed to improve with ALA the technology of plant salt tolerance, but does not report its application practice at the anti-low temperature and poor light of plant.
(3) summary of the invention
Technical problem the objective of the invention is to overcome uses plant hormone to strengthen the unstable or reduction output of degeneration-resistant effect in the prior art, especially can not solve the defective of the anti-low temperature and poor light problem of plant, a kind of plant growing and method that strengthens anti-low temperature and poor light ability of promoting is provided, be used to improve the plant photosynthesis ability, promote growth and development of plants, increase output, strengthen anti-low temperature and poor light.
A kind of plant growing and method that strengthens anti-low temperature and poor light ability of promoting of technical scheme, it is characterized in that, water root system or spray cloth blade with 0.01～20mg/L hydrochloric acid-5-amino-laevulic acid (ALA) seed soaking or in the growth of seedling phase with 1～100mg/L hydrochloric acid-5-amino-laevulic acid in the seed germination phase, continuous 1～3 day, every day 1 time.Also can use analog levulinic acid (LA) 50～500mg/L pouring root system or the spray cloth blade of 5-amino-laevulic acid in the plant seedling growth phase, continuous 1～3 day, every day 1 time.
Beneficial effect
The present invention uses a kind of single biochemical reagents ALA and analog levulinic acid thereof, promote plant growing by improving photosynthesis of plants, strengthen plant metabolism by improving respiration, increase chlorophyll content of plant (particularly chlorophyll b content), promote pore open, improve Net Photosynthetic Rate, reduce photorespiration intensity, increase soluble sugar and dry-matter accumulation, thereby not only anti-low temperature and poor light improves obviously, and can reach the purpose of volume increase.Because the 5-amino-laevulic acid can be by biological degradation originally as the endogenous metabolism product, noresidue is pollution-free, to the human body avirulence, can be used for the nuisanceless production of land for growing field crops and industrialized agriculture.
This method is easy and simple to handle, effect stability, and synergy is obvious.
The general structure of ALA and LA
Biochemical reagents hydrochloric acid-5-amino-laevulic acid and levulinic acid are generally in biochemical research (see respectively " reagent handbook) " second edition, China Drug Co.'s Shanghai chemical reagent purchasing and supply station is compiled, Shanghai science tech publishing house, 1984, the 71 pages and the 733rd page; " chemical reagent catalogue handbook Beijing chemical reagents corporation compiles, publishing house of Beijing University of Technology, 1993, the 42 pages and the 416th page).Wherein the 5-amino-laevulic acid is that tetrapyrrole comprises chlorophyll, heme, phytochrome, Cobastab in the organism
12Etc. biosynthetic key precursor, thereby in tetrapyrrole biosynthesis research process, once excessive quantity research was carried out in the biosynthesis of 5-amino-laevulic acid and physiological metabolism etc.Levulinic acid is the inhibitor of 5-amino-laevulic acid dehydrase in the organism, can suppress the ALA metabolism, promotes the ALA accumulation.But they directly apply to agricultural production, strengthen plant opposing low temperature and poor light and yet there are no report.
The present invention is the principle of plant chlorophyll and heme biosynthesis key precursor according to 5-amino-laevulic acid (ALA), discover that certain density ALA processing can obviously improve the plant leaf blade chlorophyll content, the content of chlorophyll b particularly, thereby can increase under the low light condition plant leaf blade to the absorption of luminous energy, improve blade table sightseeing quantum efficiency and carboxylation efficient, thereby improve the plant Net Photosynthetic Rate, increase dry-matter accumulation, improve soluble sugar content and cell sap concentration, also improve the dark respiration intensity of plant leaf blade or seed simultaneously, enhance metabolism, thereby, the anti-low temperature ability of plant can fundamentally be strengthened.In addition, the present invention finds that also ALA handles when improving Net Photosynthetic Rate and also suppresses photorespiration, and this may be the major reason that ALA promotes photosynthesis of plant.
Experimental study shows: ALA can obviously improve growing plants leave photosynthetic ability under the low light level, and saturated photosynthetic rate (Ps) comparison of top vane is according to exceeding 26-188%, and saturated photosynthetic rate (Ps) comparison of radical leaves is according to exceeding 40-233%.The blade Apparent quantum yield is compared respectively according to exceeding 32-271%.ALA handles and has obviously improved the low light level muskmelon blade assimilation CO of growth down
2Ability, the carboxylation efficient of blade are compared respectively according to exceeding 55-210%.The dry weight of measuring ALA processing plant shows that 10mg/L ALA and 100mg/L ALA handle back plant dry weight and compare respectively according to exceeding 17.38% and 26.91%, illustrates that the dry-matter accumulation amount increases.
Inventive features of the present invention also is, no matter be under suitable border or the low temperature and poor light condition, utilize certain density ALA to soak seed or irrigating plant seedlings root or spray and be distributed in blade, can promote plant seed germination process and growth of seedling, improve the Net Photosynthetic Rate of seedling leaves and organize dark respiration speed, strengthen the ability of the opposing low light level, low temperature environment stress.
Use a kind of plant growing and method that strengthens anti-low temperature and poor light ability of promoting of the present invention, can obviously promote photosynthesis of plant, increase adaptive capacity low temperature and poor light.Because its mechanism of action is to improve photosynthesis of plant and respiration, by increasing plant dry matter accumulation and energy reserve, promote plant growing or resist environment-stress, thereby to plant itself without any side effect.Once proposed in the past to utilize the plant hormone abscisic acid to strengthen plant cold resistance, but because abscisic acid can obviously suppress the blade stomatal aperture, thereby, when improving, plant cold resistance also suppressed photosynthesis and dry-matter accumulation.The used ALA of the present invention can increase the blade stomatal aperture under normal operation, increases space between cells CO
2Concentration improves Net Photosynthetic Rate; Plant can recover stomatal aperture and photosynthesis at short notice after standing low temperature stress, thereby, help the recovery of plant growing behind the low temperature stress.Owing to all contain the 5-amino-laevulic acid in all organisms, the farmland uses the ALA of low concentration can not cause adverse effect to ecotope, also can not cause the health safety problem, thereby, be a kind of technology of nontoxic pollution-free.
(4) description of drawings
Fig. 1 is through 6 hours muskmelon seedling of 8 ℃ of low temperature treatment, shows the injury from low temperature symptom.Left side plant only has the dehydration of a small amount of blade leaf margin to curl through the 10mg/LALA preliminary treatment, and the right plant is contrast, nearly all blade dehydration death.
(5) embodiment
Example 1. muskmelons (kind is No. 1, West Asia honey) seedling is incubated in the plant illumination incubator the about 150 μ molm of the intensity of illumination at plant top
-2S
-1When plant is immature, obvious difference is not arranged from seeing in appearance.But along with plant leaf quantity increases, the light intensity that the plant radical leaves can receive is fewer and feweri, and the result causes lower blade withered and yellow gradually, and the leaf photosynthesis performance obviously descends.The data of measuring with the photosynthetic instrument of CIRAS-1 type portable plant during 4 leaf ages show that the saturated photosynthetic rate (Ps) of the 1st blade in top is 8 μ molm
-2S
-1, the Ps of its lower blade is followed successively by 5.7,2.7 and 0.9 μ molm
-2S
-1But if blade spray cloth 10mg/L is ALA, then the Ps value of 3 days rear blades is followed successively by 10.1,9.0,7.8 and 2.3 μ molm
-2S
-1, comparison is according to exceeding 26.25%, 57.89%, 188.89% and 155.56% respectively.If blade spray cloth 100mg/L is ALA, then blade Ps value is followed successively by 11.4,9.3,5.3 and 3.0 μ molm
-2S
-1, comparison is according to exceeding 42.5%, 63.16%, 96.30% and 233.3% respectively.Because plant base portion, the illumination that it obtained is few more, and after ALA handles, radical leaves, and the amplitude of its Pn value increase is big more, illustrates that ALA can obviously improve growing plants leave photosynthetic ability (table 1) under the low light level.
Example 2. utilizes low light intensity or the low CO of the photosynthetic instrument of CIRAS-1 type portable plant in artificial control
2Measure respectively under the condition and grow in the about 150 μ molm of top light intensity
-2S
-1The Net Photosynthetic Rate of the different blades of the muskmelon seedling in the incubator is then according to light intensity-Net Photosynthetic Rate or space between cells CO
2The linear relation of concentration-Net Photosynthetic Rate calculates Apparent quantum yield and carboxylation efficient separately, the result shows, the Apparent quantum yield of adjoining tree blade illustrates that along with the decline of leaf position almost is multiple (about twice) decline muskmelon is a kind of extremely intolerant plant.If handle with 10mg/LALA, measure photosynthetic rate again after 3 days, then the comparison of the 1st leaf Apparent quantum yield is according to exceeding 40%, and each leaf exceeds 53.75%, 93.15% and 21.05% respectively under it.If handle with 100mg/L ALA in advance, then the blade Apparent quantum yield is compared respectively according to exceeding 32.98%, 133.57%, 271.23% and 160.53%.Illustrate that the ALA processing can obviously improve muskmelon blade Apparent quantum yield (table 2).
Similarly, the carboxylation efficient of muskmelon seedling leaves also descends along with the reduction of leaf position, and ALA handles the carboxylation efficient (table 2) that can obviously improve Different Leaf-position Leaf Blades.Wherein compare respectively according to exceeding 65.70% through the carboxylation efficient of 10mg/LALA processing blade, 184.62%, 59.70% and 97.17%, handle the carboxylation efficient of blade through 100mg/L ALA and compare respectively according to exceeding 96.51%, 210.77%, 55.72% and 172.64%.Show that ALA handles the muskmelon blade assimilation CO that has obviously improved growth under the low light level
2Ability.
In addition, from the leaf stomatal conductance measurement result also as can be seen, along with the reduction of plant leaf position, the blade stomatal aperture descends.This may be the result that the illumination deficiency causes the pore functional deterioration on the one hand, also is the reason that the leaf photosynthesis performance reduces on the other hand.Utilize exogenous ALA to handle and obviously to improve leaf stomatal conductance (table 2), promote the raising of photosynthetic rate.
Measure Different Leaf-position Leaf Blades chlorophyll content result and show that plant radical leaves chlorophyll content significantly is lower than the blade (table 3) than the upper part, this outward appearance symptom with the blade yellow is consistent.And the ALA processing can obviously improve leaf chlorophyll a, chlorophyll b content and chlorophyll total amount, and obviously improve the ratio of chlorophyll b and chlorophyll a, illustrate that ALA is bigger to the facilitation of chlorophyll b content, thereby help plant and under low light condition, absorb luminous energy better.
Example 3. is incubated at the about 150 μ molm of top light intensity with the muskmelon seedling
-2S
-1In the incubator, use 10mg/L ALA or 100mg/L ALA solution-treated during 4 leaf ages, measure Different Leaf-position Leaf Blades soluble sugar content and plant dry weight after 15 days, show, ALA handles the soluble sugar content that has obviously improved the muskmelon plant leaf, and wherein 10mg/LALA handles the soluble sugar content raising 43.76% that makes top the 1st leaf, and its lower blade increase rate is respectively 96.02%, 38.32% and 56.37%, overall average is 58.62%; 100mg/LALA handles and makes leaf soluble improve 104.92%, 132.05%, 34.54% and 35.79% respectively, and overall average is 76.83%.Obviously, ALA handles the leaf soluble increase has substantial connection with its photosynthetic rate raising.In addition, the dry weight of measuring ALA processing plant shows that 10mg/L ALA and 100mg/L ALA handle back plant dry weight and compare respectively according to exceeding 17.38% and 26.91%, illustrates that the dry-matter accumulation amount increases.
But it not is because ALA handles the inhibition to the dark respiration effect that the plant dry matter accumulation increases.On the contrary, the result who measures the blade respiratory rate shows (table 4), and 10mg/L ALA handles the dark respiration speed that has all improved the different joints of muskmelon seedling position blade with 100mg/L ALA, and average amplification is 40%, illustrates that ALA handles the rear blade metabolic activities and strengthens.On the other hand, measure blade photorespiration rate result and show that ALA handles and reduced muskmelon blade photorespiration rate.Handle the photorespiration of plant leaf and have only about 80% of contrast.Because photosynthesis of plant and photorespiration all are by the catalysis of RuBP carboxylation oxygenase, the ALA processing has suppressed photorespiration intensity must increase this enzyme to CO
2Affinity, so although plant dark respiration speed increases, Net Photosynthetic Rate is still obviously promoted.
Example 4. a variety of Chinese cabbage seedling are incubated at the about 150 μ molm of top light intensity
-2S
-1The plant illumination incubator in, water with 10mg/LALA or 50～500mg/L LA respectively during 4 leaf ages, each 5～10ml, for three days on end, then with the pouring of 200mmol/L NaCl solution, each 10～20ml continuous 7 days, measures the 2nd photosynthetic rate of launching blade in plant top with the portable photosynthetic instrument of CIRAS-1 type then.The result shows that 200mmol/L NaCl handles and significantly suppressed a variety of Chinese cabbage seedling leaves photosynthesis, and exogenous ALA and LA processing obviously can improve a variety of Chinese cabbage leaf photosynthesis speed under the salt stress.
No matter at light intensity-photosynthetic rate response curve (table 5) still at CO
2In-photosynthetic rate the response curve (table 6), the treatment effect of ALA and LA is all very obvious.Under the light saturation conditions, NaCl handles the plant leaf Net Photosynthetic Rate and only is 17.79% of contrast, and 10mg/L ALA processing plant leaf photosynthetic rate is 30.28% of contrast, 500mg/LLA handles plant leaf and is 42.31% of contrast, handles plant high 70.21% and 137.83% than simple NaCl respectively.Because LA can block on the one hand ALA metabolism in the plant corpus, it is synthetic and promote that therefore endogenous ALA generates to suppress heme on the other hand again, thereby LA handles and improves a variety of Chinese cabbage leaf photosynthesis speed and may increase relevant with endogenous ALA content.
Similarly, regularly measure space between cells CO when incident intensity one
2Asynchronous Net Photosynthetic Rate shows that also ALA or LA handle and obviously improve a variety of Chinese cabbage leave photosynthetic ability (table 6) that grows under the low light level and the salt stress.With space between cells CO
2Concentration is 300 μ lL
-1The time photosynthetic rate be example, the Pn value that 200mmol/L NaCl handles blade only is 10.09% of contrast, rise to 23.76% of contrast and add 10mg/L ALA rear blade Pn, the Pn value that 50～500mg/L LA handles blade is respectively 27.02% of contrast, 29.19%, 30.75%, 31.68% and 33.39%, all the utmost point is significantly higher than simple salt processing.
Example 5. muskmelon seedling are incubated in the plant illumination incubator, the about 150 μ molm of plant top light intensity
-2S
-1, cultivation temperature is 25～30 ℃, illumination every day 12 hours.During 4 leaf ages, water its root system with 10mg/L ALA solution.After three days, seedling transferred to carry out low temperature treatment in 8 ℃.Though seedling is transferred under the normal temperature condition again after 4 hours in low temperature treatment, ALA handles between plant and untreated control does not have notable difference in outward appearance, but the leaf photosynthesis speed of recovering at normal temperatures to measure after 2 hours shows, the Net Photosynthetic Rate (Pn) of all blades of ALA preliminary treatment plant all is higher than untreated control, especially the Pn value utmost point of first blade in top is significantly higher than contrast (table 7), illustrates that ALA preliminary treatment plant can prevent the injury of low temperature to the plant photosynthesis ability to a certain extent.After plant after the low temperature treatment keeps 20 hours at normal temperatures, then 50% of the not enough contrast of handling without ALA of plant leaf photosynthetic rate, and the pretreated plant leaf Pn of ALA value has returned to the control level without low temperature treatment, illustrates that the ALA preliminary treatment helps the recovery of plant photosynthesis performance behind the low temperature.
If in 8 ℃ of low temperature, handled 6 hours, then contrast blade and almost completely be water stain shape, be that the mesophyll cell collapse is dead, and ALA preliminary treatment plant almost excellent (accompanying drawing), show that ALA handles the recovery not only help plant leaf blade photosynthetic performance behind the low temperature, and can significantly improve cold resistance of plant.
Example 6. is incubated at the muskmelon seedling in the plastic tunnel of polyvinyl chloride film, and intensity of illumination is approximately 60% of nature light intensity, equally also observes the facilitation of ALA processing to leaf photosynthesis.The contrast blade is at artificial control light 300 μ molm
-2s
-1The Pn value that records under the condition is 5.9 μ molm
-2s
-1, and the Pn value that 10mg/L ALA handles is 7.1 μ molm
-2s
-1, comparison is according to exceeding 20% (table 8).At 1000 μ molm
-2s
-1The ALA that records under the light intensity condition handles the comparison of plant Pn value according to high by 40%.In addition, also observe the facilitation of ALA processing, but under this medium light intensity condition, do not observe the facilitation of ALA processing Apparent quantum yield to carboxylation efficient.This with low light condition under slightly different.ALA handles and also reduces blade photorespiration rate and CO
2Compensation point.The photorespiration that ALA handles blade only is 75% of contrast.
Example 7. carries out the ALA application test at booth muskmelon growing area, and kind is west thin Lip river holder, and the ALA working concentration is respectively 10mg/L, 50mg/L and 100mg/L, and the experimental plot area is 100m
2The result shows that ALA handles and obviously promotes output to improve, and improves fruit quality.Compared with the control, ALA handles the average per unit area yield in sub-district increases by 17.98%, 26.40% and 33.15% respectively, and the fruit soluble solid increases by 11.29%, 10.48% and 12.90% (table 9).
Example 8. is transferred to a variety of Chinese cabbage seedling of 0.5～20mg/L ALA presoaking and germinating in the vegetable plot, and every processing 10 strains were measured single-strain fresh weight after 30 days, shows that the ALA processing also can promote a variety of Chinese cabbage growth of seedling under normal operation.Wherein 20mg/L ALA handles and makes plant overground part fresh weight increase by 15～40%, and the underground part fresh weight increases by 25～60% (tables 10).Obviously, the treatment effect of ALA is significantly, and to the facilitation of root system greater than overground part.
Table 1. is through the response of 3 days muskmelon seedling Different Leaf-position Leaf Blades Net Photosynthetic Rate of ALA processing to light intensity
Incident light quantum flux density (μ molm
-2S
-1)
Handle
0 14 50 100 150 200 300 400?600?800?1000?1200?1400?1600
Contrast Net Photosynthetic Rate (μ molm
-2S
-1)
The 1st leaf-1.3-0.6 0.5 2.3 3.4 4.1 5.4 67 7.2 7.4 7.7 88
The 2nd leaf-1.4-0.6 0.4 1 1.4 1.6 2 2.6 3.1 3.4 3.6 4.4 5.4 5.7
The 3rd leaf-0.7-0.2 0.4 0.6 0.8 0.9 1.1 1.4 1.5 1.7 2 2.2 2.5 2.7
The 4th leaf-0.7 0 0.2 0.3 0.3 0.4 0.4 0.5 0.6 0.6 0.6 0.7 0.8 0.9
10mg/L?ALA
The 1st leaf-1.2-0.7 13 4.9 6.3 8.3 9 9.3 9.5 9.7 10.1 10.1 10.1
The 2nd leaf-0.8 0.1 1.4 3.1 4.6 5.6 6.9 7.8 8.3 8.4 8.5 8.7 8.8 9
The 3rd leaf-0.6 0.1 1.2 2.6 3.9 4.7 5.6 6.3 6.6 6.8 6.9 7.1 7.5 7.8
The 4th leaf-0.4-0.3 0.3 0.4 0.5 0.5 1.2 1.4 1.6 1.6 1.6 1.7 2.2 2.3
100mg/L?ALA
The 1st leaf-1.5-1.1 0.3 2.5 4.2 5.7 7.5 9.3 9.7 9.8 10.3 10.8 11.2 11.4
The 2nd leaf-1.2-0.7 1 1.7 2.5 3.2 4.8 5.5 6.3 6.6 7.4 8.7 9 9.3
The 3rd leaf-0.8 0 0.6 1.3 1.8 2.2 2.6 3.1 3.7 4 4.2 4.6 4.8 5.3
The 4th leaf-0.4 0 0.4 0.6 1.4 1.6 2 2.1 2.3 2.4 2.5 2.6 2.9 3
Table 2.ALA handles the facilitation to the photosynthetic Apparent quantum yield of muskmelon seedling, carboxylation efficient and leaf stomatal conductance
Apparent quantum yield carboxylation efficient stomatal conductance Gs (mmolm
-2s
-1)
The leaf position
Contrast 10mg/L ALA 100mg/L ALA contrast 10mg/L ALA 100mg/L ALA contrast 10mg/L ALA 100mg/L ALA
The 1st leaf 0.0282 0.0395 0.0375 0.0344 0.0570 0.0676 100.77 161.38 178.46
The 2nd leaf 0.0140 0.0215 0.0327 0.0195 0.0555 0.0606 30.38 68.23 132.15
The 3rd leaf 0.0073 0.0141 0.0271 0.0201 0.0321 0.0313 16.69 37.77 76.62
The 4th leaf 0.0038 0.0046 0.0099 0.0106 0.0209 0.0289 10.23 27.08 28.92
Table 3.ALA handles and improves the muskmelon chlorophyll content in leaf blades
Chlorophyll content (mg/gFW)
The first leaf Chl a Chl b Chl a+b Chl b/a
Contrast 0.9571 0.4871 1.4438 0.5079
10mg/LALA 1.2599 0.6618 1.9212 0.5256
100mg/LALA 1.3866 0.7887 2.1747 0.5683
The 2nd leaf
Contrast 0.9784 0.4804 1.4585 0.4842
10mg/LALA 1.2118 0.6372 1.8485 0.5240
100mg/LALA 1.2923 0.7060 1.9977 0.5462
The 3rd leaf
Contrast 1.0299 0.5123 1.5418 0.4968
10mg/LALA 1.2110 0.6284 1.8390 0.5188
100mg/LALA 1.2106 0.6365 1.8466 0.5237
The 4th leaf
Contrast 0.7927 0.3936 1.1863 0.4965
10mg/LALA 0.9167 0.4637 1.3801 0.5343
100mg/LALA 1.0949 0.5890 1.6839 0.5379
Table 4.ALA handles the influence to respiratory rate under the accumulation of muskmelon blade solubility, dark respiration speed and the light
Soluble sugar content (mg/gFW) dark respiration speed (μ molm
-2S
-1) photorespiration rate (μ molm
-2S
-1)
The leaf position
Contrast 10mg/LALA 100mg/LALA contrast 10mg/LALA 100mg/LALA contrast 10mg/LALA 100mg/LALA
The 1st leaf 0.914 1.314 1.873 1.183 1.256 1.322 5.952 5.448 5.354
The 2nd leaf 0.855 1.676 1.984 0.801 1.113 1.220 4.621 3.732 3.519
The 3rd leaf 1.216 1.682 1.636 0.618 1.231 1.014 3.296 2.834 2.973
The 4th leaf 1.084 1.695 1.472 0.363 0.562 0.607 3.042 2.023 1.935
Table 5. exogenous ALA or LA handle the influence (CO to a variety of Chinese cabbage blade Net Photosynthetic Rate under the salt stress
2Concentration is constant, and incident intensity is 0～1000 μ molm
-2S
-1)
Handle incident intensity (μ molm
-2S
-1)
0 14 50 100 200 300 400 600 800 1000
Contrast-0.80-0.05 2.00 4.05 5.95 6.95 7.90 8.70 9.65 10.4
200mmol/LNaCl -0.30 -0.15 0.50 1.20 1.50 1.55 1.65 1.76 1.85 1.85
10mg/L?ALA+NaCl -0.35 -0.15 0.80 1.65 1.85 2.55 2.60 2.95 3.1 3.15
50mg/L?LA+NaCl -0.30 -0.10 0.55 1.32 1.63 1.74 1.82 2.05 2.10 2.23
100mg/L?LA+NaCl -0.35 -0.15 0.65 1.45 1.78 1.85 2.05 2.34 2.55 2.60
200mg/L?LA+NaCl -0.35 -0.15 0.70 1.65 1.80 2.15 2.40 2.75 2.90 3.03
300mg/L?LA+NaCl -0.33 -0.15 0.85 1.86 2.55 2.86 3.24 3.45 3.55 3.55
500mg/L?LA+NaCl -0.32 -0.15 1.00 2.00 2.85 3.2 3.55 3.65 4.35 4.40
Table 6. exogenous ALA or LA handle influence to a variety of Chinese cabbage blade Net Photosynthetic Rate under the salt stress, and (incident intensity is 400 μ molm
-2S
-1, space between cells CO
2Be 25～800 μ lL
-1)
Handle space between cells CO
2(μ lL
-1)
25 50 100 150 200 300 400 600 800
Contrast-2.50-1.10 1.25 2.84 4.53 6.44 8.25 9.74 10.23
200mmol/L?NaCl -1.40 -0.75 -0.45?-0.45?0.16 0.65 1.25 1.60 2.00
10mg/L ALA+NaCl -1.15 -0.90 -0.50?0.35 0.67 1.53 2.36 2.67 2.93
50mg/L LA+NaCl -1.25 -0.75 -0.36?0.55 0.98 1.74 2.43 2.95 3.44
100mg/L?LA+NaCl -1.36 -0.65 -0.25?0.75 1.23 1.88 2.56 3.15 3.67
200mg/L?LA+NaCl -1.35 -0.70 -0.12?0.80 1.34 1.98 2.75 3.45 3.89
300mg/L?LA+NaCl -1.40 -0.66 0.22 0.95 1.45 2.04 2.96 3.66 4.21
500mg/L?LA+NaCl -1.45 -0.70 0.4 1.10 1.55 2.15 3.05 3.80 4.35
Table 7. through 4 hours muskmelon seedling of 8 ℃ of low temperature treatment in normal temperature, recover 2 hours with 20 hours after the Net Photosynthetic Rate of Different Leaf-position Leaf Blades, show that the ALA preliminary treatment recovers the effect of leaf photosynthesis performance
Recover 20 hours Net Photosynthetic Rate in 2 hours the Net Photosynthetic Rate normal temperature of recovery in the normal temperature
The leaf position
Contrast 10mg/L ALA low temperature treatment ALA+ low temperature contrast 10mg/L ALA low temperature treatment ALA+ low temperature
The 1st leaf 6.20 9.35 1.00 2.40 8.15 9.13 3.85 8.20
The 2nd leaf 6.30 9.50 0.90 1.60 4.65 7.63 1.85 3.90
The 3rd leaf 3.23 5.05 0.65 1.00 2.95 4.77 1.35 2.65
The 4th leaf 2.50 3.65 0.30 0.55 2.05 2.67 0.80 1.55
Table 8. booth muskmelon seedling is at artificial control light intensity (0～1000 μ molm
-2s
-1) following measured Net Photosynthetic Rate (Pn)
Incident intensity (μ molm
-2s
-1)
Handle
0 15 50 100?150 200 300 400 500 600 700 800 900 1000
Contrast-1.3-0.5 1.5 2.7 4.0 4.9 5.9 6.6 7.5 7.6 8.5 8.9 9.0 9.6
10mg/L?ALA?-2.0?-0.8?1.4 3.1?4.4 5.0 7.1 8.7 9.8 10.7?11.7?12.5?12.7?13.5
Table 9.ALA handles the influence to muskmelon output and quality
Per mu yield (kg/666.7m
2) soluble solid %
Contrast 890 12.4
10mg/LALA 1050 13.8
50mg/LALA 1125 13.7
100mg/LALA 1185 14.0
Table 10. variable concentrations ALA handles the promotion effect to a variety of Chinese cabbage growth of seedling under the normal field condition
Handle contrast 0.5mg/L ALA 1mg/L ALA 10mg/L ALA 20mg/L ALA
Overground part fresh weight 15.00 15.26 15.48 15.59 17.32
(g/ strain) (100%) (101.73%) (103.20%) (103.93%) (115.47%)
Test 1
Underground part fresh weight 3.28 3.83 3.75 4.14 4.11
(g/ strain) (100%) (116.77%) (114.33%) (126.22%) (125.30%)
Overground part fresh weight 10.66 11.61 12.88 13.74 14.82
(g/ strain) (100%) (108.91%) (120.83) (128.89%) (139.02%)
Test 2
Underground part fresh weight 3.87 5.01 5.55 5.76 6.15
(g/ strain) (100%) (129.46%) (143.41%) (148.84%) (158.91%)
Claims (1)
1. one kind promotes plant growing and the method that strengthens anti-low temperature and poor light ability, it is characterized in that, water root system or spray cloth blade, continuous 1～3 day, every day 1 time with 0.01～20mg/L hydrochloric acid-5-amino-laevulic acid (ALA) seed soaking or in the growth of seedling phase with 1～300mg/L in the seed germination phase;
Perhaps water root system or spray cloth blade, continuous 1～3 day, every day 1 time with 50～500mg/L levulinic acid (LA) in the plant seedling growth phase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02138598XA CN1172593C (en) | 2002-11-15 | 2002-11-15 | Method of pronoting plant growth and enhancing anti reverse property |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02138598XA CN1172593C (en) | 2002-11-15 | 2002-11-15 | Method of pronoting plant growth and enhancing anti reverse property |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1409962A CN1409962A (en) | 2003-04-16 |
| CN1172593C true CN1172593C (en) | 2004-10-27 |
Family
ID=4749580
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB02138598XA Expired - Fee Related CN1172593C (en) | 2002-11-15 | 2002-11-15 | Method of pronoting plant growth and enhancing anti reverse property |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1172593C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409062A (en) * | 2010-09-20 | 2012-04-11 | 中国科学院植物研究所 | Method for changing content of chlorophyll in plant |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2766499C (en) * | 2009-06-29 | 2018-05-22 | Bayer Bioscience N.V. | Improvement of yield in crop plants through selection of epigenetically modified populations |
| IN2012DN05886A (en) * | 2009-12-07 | 2015-09-25 | Univ Kyoto | |
| CN101863703B (en) * | 2010-06-21 | 2012-11-21 | 南京农业大学 | High-efficiency amino acid liquid bio-fertilizer |
| CN102718849B (en) * | 2011-03-30 | 2014-04-09 | 中国科学院植物研究所 | Protein related to chlorophyll synthesis and coding gene and application thereof |
| CN102860194B (en) * | 2011-07-07 | 2015-03-04 | 福建南方制药股份有限公司 | Planting method of taxus chinensis and application of 5-aminolevulinic acid |
| CN102783344A (en) * | 2012-08-22 | 2012-11-21 | 吴庭贵 | Method for cultivating and planting mulberries in cold area |
| CN103193875B (en) * | 2013-04-10 | 2015-03-18 | 中国农业科学院作物科学研究所 | Paddy chloroplast growth related protein as well as coding gene and application thereof |
| CN103975735B (en) * | 2014-05-13 | 2016-02-10 | 谭国华 | A kind of tulip seed balls breeds rejuvenation production method |
| CN104798618B (en) * | 2015-05-08 | 2018-04-10 | 南京禾稼春生物科技有限公司 | A kind of application for the method and 5 amino-laevulic acids for alleviating the injury of fig seedling flood stain |
| CN105594734B (en) * | 2015-10-12 | 2017-09-08 | 中国农业科学院作物科学研究所 | The anti-fall health care specular removal yield-increasing modifying agent of corn, its preparation method and its application |
| CN105973851B (en) * | 2016-06-12 | 2018-10-23 | 中国科学院地球化学研究所 | A kind of bathmic method of quantitative determination plant photosynthesis |
| CN107736368A (en) * | 2017-09-13 | 2018-02-27 | 西南大学 | 5 amino-laevulic acids and purposes of the CPPU mix preparation for planting sheep's hay |
| CN108419474B (en) * | 2018-01-15 | 2020-10-09 | 长江大学 | Method for increasing alpha-bisabolol content of chamomile |
| CN108522503B (en) * | 2018-04-16 | 2020-06-30 | 中国农业科学院蔬菜花卉研究所 | Cucumber growth regulator and sunlight greenhouse cucumber planting method |
| CN113785832B (en) * | 2021-08-24 | 2022-03-29 | 南京农业大学 | Application of 2-amino-3-methyl caproic acid in promoting plant growth and increasing yield |
| CN114199714B (en) * | 2021-11-22 | 2022-05-20 | 中国农业大学 | Method for high-pass identification and selection of plant resistant varieties by integrating physiological indexes of germination stage growth and seedling stage |
| CN115968883A (en) * | 2022-12-07 | 2023-04-18 | 郑州郑氏化工产品有限公司 | Composition containing 5-aminolevulinic acid and nicotinamide ester and application thereof |
-
2002
- 2002-11-15 CN CNB02138598XA patent/CN1172593C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102409062A (en) * | 2010-09-20 | 2012-04-11 | 中国科学院植物研究所 | Method for changing content of chlorophyll in plant |
| CN102409062B (en) * | 2010-09-20 | 2013-12-11 | 中国科学院植物研究所 | Method for changing content of chlorophyll in plant |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1409962A (en) | 2003-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1172593C (en) | Method of pronoting plant growth and enhancing anti reverse property | |
| Allahverdiyev et al. | Effect of drought stress on some physiological parameters, yield, yield components of durum (Triticum durum desf.) and bread (Triticum aestivum L.) wheat genotypes | |
| CN103923967A (en) | Comprehensive utilization method of straw | |
| CN104860738A (en) | Biomass charcoal-based seedling growing matrix and preparation method thereof | |
| CN1305367C (en) | Northern herbal, bush and arbor anti-contravariance plant mixed seeds | |
| CN104163719B (en) | A kind of preparation method containing aminoacid Water soluble fertilizer | |
| CN1706239A (en) | Walnut twig cutting propagation technology | |
| Adzman et al. | Preliminary study on Azolla cultivation and characterization for sustainable biomass source | |
| CN112974505B (en) | Method for restoring rice field soil cadmium pollution by using activator and nitrogen-fixing blue algae | |
| CN107041181B (en) | Method for promoting growth of pea seedlings and reducing cadmium accumulation | |
| Landesman et al. | Nutritional value of wastewater-grown duckweed for fish and shrimp feed | |
| CN101036467A (en) | Nutritional type ginseng growth regulator | |
| CN105660466A (en) | Method for breeding grouper fry by controlling illumination | |
| CN1944576A (en) | Saline-alkali soil amemdment, its preparing method and use | |
| CN1762205A (en) | Generation method in the bottle of oriental hybrid lily detoxified small seed ball | |
| CN104641999A (en) | Method for manufacturing celery floating seedling culturing substrate based on mushroom residue | |
| CN1262186C (en) | Insect-preventing harmless ecological liquid fertilizer | |
| CN1926986A (en) | Plant nutriment regulator | |
| CN104756839B (en) | Using tomato stalk as tomato seedling substrate of raw material and preparation method thereof | |
| CN1191466A (en) | Improving yield of plants | |
| CN105967781A (en) | Functional organic fertilizer with effects of fertilizing soil and expelling parasites | |
| CN1200650A (en) | Improving yield of plants | |
| Adamec | Dark respiration of leaves and traps of terrestrial carnivorous plants: are there greater energetic costs in traps? | |
| CN106905042A (en) | Fermenting bed padding conditioner | |
| CN1401613A (en) | Method for producing biological active organic fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |