CN1191466A - Improving yield of plants - Google Patents

Improving yield of plants Download PDF

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Publication number
CN1191466A
CN1191466A CN95197917A CN95197917A CN1191466A CN 1191466 A CN1191466 A CN 1191466A CN 95197917 A CN95197917 A CN 95197917A CN 95197917 A CN95197917 A CN 95197917A CN 1191466 A CN1191466 A CN 1191466A
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betain
crop
cereal
output
purposes
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E·维尔塔男
E·派尤
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Finphide OY
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Cultor Oyj
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
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  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)
  • Fertilizers (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention relates to the exogenous use of betaine to improve the yield of C-4 cereals. According to the invention, betaine is used to improve the yield especially under stress conditions. The invention also relates to C-4 cereals treated exogenously with betaine, particularly to the seeds of such plants.

Description

Improve the method for crop yield
Technical field
The present invention relates to be used to improve the purposes of the betain of crop yield.The present invention is specifically related to the purposes in order to the betain that improves the C-4 grain yield.According to the present invention, both can also can under mal-condition, improve output under normal operation, disturb the mal-condition of growth to comprise such as arid, high salinity, low temperature is moist or have an environmental poisonous substance.The present invention also relates to the C-4 cereal handled through betain and the part (especially seed-grain) of described cereal, and by the product of aforementioned preparation.
Background
The environment of growth and the output of condition appreciable impact crop.Growing environment and the condition optimized are followed good quality and high output usually.Under bad growth conditions, quality and output worsen thereupon.
The physiological property of crop preferably by breeding method control, both can be traditional breeding method, also can be the method such as genetic recombination.
So far, developed the solution of several different relevant cultivation technology, in order to improve growth conditions and to improve crop yield.To those skilled in the art, be self-evident the suitable corresponding crop of the seed selection of point vegetatively.In vegetative period, can pass through the mechanical means cover crop, as various tulles or plastics, or in the greenhouse, cultivate crop.For improving output, use usually and irrigate and fertilizer.The also normal and agricultural chemicals of surfactant, protectant and mineral matter are used simultaneously.Surfactant has improved the infiltration of material to plant cell, has therefore improved and increased the effect of mentioned reagent, has also reduced its illeffects to environment simultaneously., various method usually is an effort and unpractical in the cultivation technology, and its effect is also limited, and (the greenhouse size is limited; the protection that tulle provides is limited; or the like), more because these methods in large scale when implementing, corresponding cost is much also expensive.At present, Shang Weijian has economically viable chemical solution, avoids the influence of severe environmental conditions in order to cover crop.
Different Crop is to the susceptibility difference of drying, but for the productive rate of crop, the supply of water is more important than other any environmental factor.Usually use and irrigate the supply that is enough to guarantee water., irrigation is closely related with health and environmental problem, and for example the extreme of water resource descends, the deterioration of water quality and the deterioration of agricultural land.Calculate factually, about in the world half people is irrigateed land the infringement that is subjected to damage or crop failure caused by waterlogging and salinization.The seriousness of this problem and the scope that relates to thereof are embodied in, and have 255,000,000 hectare to irrigate land in the world, and they account for 70% of whole world water consumption.Only, mainly just have to surpass 20,000,000 hectare irrigated land in the southeast of 18 areas, western state and this state in the U.S..The water consumption that these areas only are used to irrigate just accounts for 83% of whole water consumptions.Other it should be noted that the consumption of irrigation water increases year by year, especially in industrial country.With the exception of this, another shortcoming of irrigation is the cost height.
Another serious abominable factor is the salinity of soil.Can define the salinity of soil with diverse ways; According to common definition, such as the content of solvable salinity in the soil reach be enough to disturb in the cultivation crop when growth of several crop species and output, then say soil salinization.Modal salt is sodium chloride, but relevant with the dissolubility of the source of salt solution and salt, and other salt also exists with different compound modes.
For being grown in the crop that contains saline land, it is difficult obtaining enough water from the soil that negative osmotic pressure is arranged.The sodium and the chlorion of high concentration are harmful to crop.Another problem is the shortage of mineral, and this appears at the occasion of sodium ion and the competition of required potassium ion, yet, for the cell growth, need osmotic adjustment and pH stable.Especially when calcium ion concentration is low this problem can appear.
The output capacity of crop and also relevant with crop species to the susceptibility of soil salinity.Halophytes needs high relatively sodium chloride content with the assurance optimal growth, and glycophyte is low to the tolerance of salt, even its growth also is subjected to remarkable inhibition when salinity is low.To same cultivation crop species, between the different cultivation kinds even big difference also arranged.Same crop species or cultivation kind of the same race to the tolerance of salt also with changing such as vegetative stage.When low or medium salinity, the growth more slowly of glycophyte does not manifest with the special marquis's of levying form (as chlorosis), but it is slow to show plant growth, and the color of leaf is more normally for black.And the gross area of leaf descends, and carbon dioxide assimilation reduces, and protein synthesis is suppressed.
On certain degree, crop can adapt to harsh conditions.This ability and crop species are closely related.Because aforementioned mal-condition, some crop begins to produce the somatotropin of a kind of title abscisic acid (ABA), so as to the pore of sealing crop, thereby reduces the injury of mal-condition., ABA also has adverse side effect to the output capacity of crop.ABA causes for example leaf, and flower and immature fruit fall and suppress the formation of new leaf, and this causes the decline of output naturally.
Have found that the harsh conditions especially shortage of water can cause the serious decline of some enzymic activity, as nitrate reductase and phenylalnine ammonialyase.On the other hand, α-Dian Fenmei and ribonuclease activity increase.So far do not see the chemical solution of finding cover crop based on these is arranged.
Find also that in addition under harsh conditions, some nitrogen-containing compound and amino acid (as proline and betain) accumulate at the growth district of certain plants.The documents of prior art the function and the meaning of these accumulations.Think that these products are accessory substances of mal-condition on the one hand, so pair cell is harmful; Infer that on the other hand they can protect cell (Wyn Jones R.G. and Storey R work.Plant drought physiology and biochemistry [The Physiology and Biochemistry of DroughtResistance in Plants], Paleg L.G. and Aspinall D edit, academic press [Academic Press], Sydney, Australia, 1981).
Zhao etc. (plant physiology magazine [J.Plant Physiol],, volume 140, page or leaf 541~543 in 1992) have described the influence of betain to the clover cell membrane.Spray the clover seedling with the glycinebetaine of 0.2M, the seedling that from substrate, roots out afterwards, flush away soil and to be exposed to temperature-10 ℃~-2 ℃ following 1 hour.Thaw then seedling and on moist sand ground the plantation one week, therebetween, the survival crop regrowth obvious.Glycinebetaine improves the cold stability of clover significantly.Cold treatment effect at-6 ℃ is especially obvious.All-6 ℃ of cold treatments one hour to all dead in the same old way, and 67% survival is arranged with the seedling that glycinebetaine is handled.
Itai and Paleg (plant science communication [Plant Science Letters], nineteen eighty-two, volume 25, page or leaf 329~335) have described the effect that proline and betain recover the barley and the cucumber of olighydria.Plant growth in order to cause olighydria, in nutrient solution added polyethylene glycol (PEG, 4000mol weight) in continuous four days in the sand ground of flushing, allowed crop recover four days before harvesting afterwards.First day or the 3rd day of olighydria, or be right after before the harvesting, to the leaf of crop spray proline and/or betain (25mM, pH6.2).According to observation, before or after lacking, provide betain not take effect really, and added betain is effective when lacking end to barley; Proline is invalid.To cucumber, do not see tangible facilitation effect; On the contrary, find that betain and proline all have detrimental effect.
As seen, be intended to distinguish betain and proline the experiment of crop effect has been produced opposite result, so corresponding results does not have commercial value.
The invention summary
The objective of the invention is to seek a kind of part and replace artificial method of irrigating, meanwhile, guarantee the quality and the output of product.Another object of the present invention is, seek at other mal-condition (as usual and dry relevant high salt, low temperature etc.) but under the also method of cover crop.And further target is, finds and does not use the method that can consume environmental resource or environmentally hazardous method but can increase output under normal operation.
An astonishing discovery related to the present invention is, can be by executing the output that betain significantly improves C-4 cereal outward.Find that betain is all effective to improving output under normal and harsh conditions, and, the harmful effect as the ABA side effect do not had.The present invention might significantly reduce the demand such as man-made irrigation, thereby the protection environment also significantly reduces cost.
So the betain that the present invention relates to improve the C-4 grain yield is used the way outward.
The present invention is specifically related to improve the betain purposes of C-4 cereal seed-grain output.
According to the present invention, execute betain all can improve C-4 cereal under normal and harsh conditions output outward.
The present invention and then relate to the C-4 cereal of outside betain, executing processing and the part (especially seed-grain and fringe) of described cereal, and described material is such as the purposes in food and the animal feed industry.
The invention still further relates to the method that improves the C-4 grain yield, in this method, betain imposes on the C-4 cereal in the growth outward.
Betain is applied on the crop through one or several continuous treatment step.Use and to finish by for example spraying, and where necessary, spray with the fertilizer of routine or agricultural chemicals etc.The betain of using according to the present invention is transported to plant cell, and the osmotic equilibrium of regulating cell energetically in the cell place, also participates in other cellular process.Even the cell of garden beet alkaline treatment also more has vigor when suffering outside abominable factor.
According to the present invention, handling through betain is favourable economically, and the output that increases also has economic benefit.Can finish with other spraying owing to handle, it can obviously not increase workload, and need be at machinery, and new investment is carried out in equipment or aspect, space.It should be noted that in addition betain is nontoxic natural prodcuts, the quality of product is had no adverse effect.Betain also is the stable material that remains in the plant cell, and therefore long-term effect is arranged.
Detailed Description Of The Invention
Betain refers to the methylated amino acid of complete N-.Betain is the natural products that critical function is arranged in the metabolism of plant and animal.One of prevailing betain is a glycine derivative, and three methyl wherein are connected with the nitrogen-atoms of glycine molecule.This betaine compound is betain usually, glycinebetaine or trimethylglycine, and its structural formula is as follows:
Figure A9519791700071
For example, other betain comprises alanine betain and proline betain, and report is arranged, and this two alkali can prevent the perosis such as chicken.Show " plant drought physiology and biochemistry [The Physiology and Biochemistry of DroughtResistance in Plants] " (Paleg L.G. and Aspinall D edit at Wyn Jones R.G. and Storey R, academic press [Academic Press], Sydney, Australia, 1981) the detailed introduction of betain is arranged in the book, this publication is also introduced as reference herein in the lump.
Betain has one two electrode structure and contain the chemically active methyl of the several tools that can confess in enzymic catalytic reaction.Most of organic physical efficiencys are synthesized a small amount of betain, are used for for example methyl function, but this can not significantly increase the growing amount and the storage capacity of betain, to resist abominable external condition.The most known organism that betain accumulates comprises Chenopodiaceae family crop (for example sugar beet), certain micro-organisms and oceanic invertebrate.In these organisms, the main cause that betain accumulates may be that betain plays infiltration matter, thereby the protection cell is avoided the influence of osmotic pressure.When condition needed, for example when high salt or drying, one of betain major function in these crops and the microorganism was the infiltration capacity that increases cell, prevents moisture loss.Different with many salt, betain and enzyme highly compatible, so the content of the betain in the organelle of cell and cell can be high, but metabolism is not had any illeffects.Find also that in addition betain has stabilization to macromolecular motion; It improves the thermal resistance of enzyme and cell membrane and to the tolerance of ion.
Betain can be from for example extracting with chromatography the sugar beet.Betain can be Cultor Oy, the commercially available prod of Finnsugar Bioproducts, and the latter binds brilliant dehydration betain.Other betain product comprises such as the betain monohydrate, and beet alkali hydrochlorate and thick beet alkali lye all can derive from commercially available and is used for purpose of the present invention.
According to the present invention, execute betain outward to improve the output of C-4 cereal (as corn, Chinese sorghum, millet, SEDGE, BUFFALGRASS, DRABGRASS, WITCHGRASS etc.).According to the present invention, under normal and harsh conditions, execute betain outward improving the output of C-4 cereal, even when the crop of cultivating suffers periodicity or continuous external pressure, betain also has effectiveness.Such external pressure factor for example comprises dry, high temperature, high salt, air pollution (as ozone, nitrogen oxide, sulphur dioxide and sulfuric acid or say acid rain), environmental poisonous substance, weed killer herbicide, agrochemical.For example, execute betain outward and handle the crop suffer mal-condition, can improve crop to the adaptability of condition and that its growth potential is continued is longer, thereby the output capacity of raising crop.Betain is the stable material that remains in the crop cell.So the beneficial effect of betain is long-acting and only dilutes minimizing gradually due to plant growing.
Though this explanation and claim are used term " betain ", apparent, can adopt several different betains according to the present invention in case of necessity.Should also be noted that this place speech betain is a generic noun, comprise various known betains.
Betain with once or the processing of several successive step be applied to crop, single agent is used to preferably.Consumption is according to C-4 cereal kind and cultivate kind, and vegetative stage and condition are determined.For example, effective dose is the about 0.2~20kg betain of per hectare, correspondingly, and the about per hectare 2~6kg of preferable amount betain.The amount that herein provides is suggestive; Therefore, scope of the present invention comprises effective all consumptions under the working method described herein.
Betain use the method that can adopt any suitable the object of the invention.Betain can with other plant protection product, insecticide or nutrient as fungicide and urea or micro-nutrient, are used respectively or together.Betain is easy to pass through spray application.Execute betain and other possible reagent is method for optimizing to foliar spray, this response than the root application process is more rapid.Yet, also have the different problem relevant with this method, low as the osmotic concentration of the thicker leaf of crust, hydrophobic surface dry up rainwash, the rapid draing of solution, and the infringement of leaf.If necessary, other method also can be used for betaine administration.
According to the present invention, preferably use the aqueous solution of betain.
According to the present invention, the time of processing can change.If, handle usually and finish in early days, for example for about crop of 5 to 20cm in growth with single processing step betaine administration.As using with the processing of the several successive step, last spraying is preferably being bloomed early stage or is being carried out when weather forecast has heavy weather.
After betaine administration is handled according to the present invention, improved the output of crop widely, for example the quality and quantity of crop.Handle the demand that also can reduce to irrigating according to the present invention.The treatment in accordance with the present invention economical rationality, and the increase of output has economic benefit.For example, the present invention shows that rationally maize yield increases more than 30% after the betaine administration, is roughly equal to 6kg/ha.Also merit attention is that after output significantly improved, quality there is no deterioration.
According to the present invention, both can also can under mal-condition, improve the output of C-4 cereal under normal operation, mal-condition also comprises such as the high salinity relevant with arid except that comprising arid, high temperature, or the like.And the present invention also can make on the soil that the C-4 corn growing formerly thinks to be unsuitable for cultivating.
The present invention will further encyclopaedize in the following embodiments.Embodiment only supplies illustration the present invention, and they under any circumstance should not be considered to limit the scope of the invention.
Embodiment 1 betaine administration is to the influence of Chinese sorghum output
(Perth Australia) has investigated the influence of betaine administration to Chinese sorghum output in Murdoch university.Test is carried out under the condition on the spot, and the time is 1994~nineteen ninety-five in xeothermic summer.
Test is carried out with the piecemeal design, and every is 10m 2Each piece is divided into four sub-pieces again, handles with the betain of variable concentrations respectively.The concentration of used betain is: 0 (for contrast), 2,4 and 6kg/ha.Soil is sandy (98% sand, 1% silt, 1% clay), nitrogen, the content of phosphorus and potassium is low, and water and nutrient possess poor performance.Irrigation volume is normal.The cultivation kind is Trump.The results are shown in table 1.
Table 1 betaine administration is to the influence of Chinese sorghum output
Irrigation volume (%) Betain amount of application (kg/ha) I is average for betain seed output (kg/ha) piece I piece
??100 0 (for contrast) 246 ????2091????2201????2146 ????2207????2302????2254 ????2267????2354????2310 ????2347????2435????2391
Above result shows, the output of all tests of being carried out is all above in the same old way output.The betain amount of application is 4 or during 6kg/ha, best results.
Betaine administration is to the influence of Chinese sorghum output under embodiment 2 drying conditions
Repeat embodiment 1 described test, investigated the influence that betaine administration is grown to Chinese sorghum under the olighydria condition, but irrigation volume is optimized consumption minimizing 50%.The result is as shown in table 2.
Betaine administration is to the influence of Chinese sorghum output under the table 2 olighydria condition
Irrigation volume (%) Betain amount of application (kg/ha) I is average for betain seed output (kg/ha) piece I piece
??50 0 (for contrast) 246 ????1891????1913????1902 ????2105????2018????2061 ????2185????2089????2137 ????2059????2185????2122
Obviously, the output in this test also surpasses the output in the same old way.Be also shown in, according to betaine administration of the present invention but irrigation volume low (50%), effect but with Optimization of Irrigation under quite.This shows that also reduction is irrigated but used relatively large betain at the same time and can obtain same Isoquant.
Embodiment 3 betaine administration are to the influence of corn yield
(Perth Australia) has investigated the influence of betaine administration to corn yield in Murdoch university.Test is carried out under the condition on the spot, and the time is 1994~nineteen ninety-five in xeothermic summer.
Test is carried out with the piecemeal design, and every is 10m 2Each piece is divided into four sub-pieces again, handles with the betain of variable concentrations respectively.The concentration of used betain is: 0 (for contrast), 2,4 and 6kg/ha.Soil is sandy (98% sand, 1% silt, 1% clay), nitrogen, the content of phosphorus and potassium is low, and water and nutrient possess poor performance.Irrigation volume is normal.The cultivation kind is SR-73.The results are shown in table 3.
Table 3 betaine administration is to the influence of corn yield
Irrigation volume (%) Betain amount of application (kg/ha) I is average for betain seed output (kg/ha) piece I piece
??100 0 (for contrast) 246 ??4129????4342????4235 ??4380????4710????4545 ??4591????4754????4672 ??4972????5046????5009
Above result shows, the output of all tests of being carried out is all above in the same old way output.When the betain amount of application is 6kg/ha, best results.
Betaine administration is to the influence of corn yield under embodiment 4 drying conditions
Repeat embodiment 3 described tests, investigated under the olighydria condition betaine administration, but irrigation volume is optimized consumption and reduced 50% the influence of corn growth.The result is as shown in table 4.
Betaine administration is to the influence of corn yield under the table 4 olighydria condition
Irrigation volume (%) Betain amount of application (kg/ha) I is average for betain seed output (kg/ha) piece I piece
???50 0 (for contrast) 246 ??3202????3204????3203 ??3704????3882????3793 ??3815????4484????4149 ??4179????4394????4286
Equally, apparent, the output in this test is also above in the same old way output.The betain amount of application is 4 or during 6kg/ha, best results.Be also shown in, according to betaine administration of the present invention but irrigation volume low (50%), effect but with Optimization of Irrigation under quite.This shows that also reduction is irrigated but used relatively large betain at the same time and can obtain same Isoquant.
Embodiment 5 betaine administration are to the influence of early growth
With water in contrast, investigated the influence of betain to the corn early growth.Corn seed classification is Jubilee Lot#1987-14, originates from Northrup King Co..In the test, prepare five kinds of different testing liquids:
Testing liquid pH
A de-ionate 7.01
B betain (0.02g/L) 6.34
C betain (2g/L) 6.80
Betain is Betafin BC, and Finnish Sugar Co. produces.
Get 20 corn seeds, in one of 330mL afore-mentioned test solution, soaked 24 hours.Subsequently, seed is dry on the net at stainless steel sift, reseeds in the soil, two branch kinds is wherein arranged in container.Container places on the windowsill then, and face south is subjected to sunshine to shine photograph, and all water every day.
After 10 days on-tests, measure the height that sprouts.Measure after 19 days on-tests for the second time and carry out.
The result shows that betain promotes that plant germinates fast.The results are shown in Table 5.
Table 5 betaine administration is to the influence of corn early growth
Betain application concentration (g/L) Height (inch) sprouts Contrast increases (%)
The A contrast ????2.31 ????100
B betain 0.02 ????4.34 ????187
C betain 2g/L ????3.16 ????137

Claims (15)

1. for improving the purposes that C-4 grain yield institute executes betain outward.
2. according to the purposes of claim 1, it is characterized in that betain is used under mal-condition.
3. according to the purposes of claim 2, it is characterized in that mal-condition comprises high or low temperature, arid or high salinity.
4. according to each purposes of claim 1 to 3, it is characterized in that the betain amount of application is about 0.2~20kg/ha.
5. according to the purposes of claim 4, it is characterized in that the betain amount of application is about 2~6kg/ha.
6. improve the method for C-4 grain yield, it is characterized in that betain imposes on the C-4 cereal in the growth outward.
7. according to the method for claim 6, it is characterized in that betain is applied to the C-4 cereal that is grown under the mal-condition.
8. according to the method for claim 6 or 7, it is characterized in that mal-condition comprises high or low temperature, arid or high salinity.
9. according to each method of claim 6 to 8, it is characterized in that betain was used once or for several times in the season of growth.
10. according to each method of claim 6 to 9, it is characterized in that betain is used with certain insecticide or surfactant.
11., it is characterized in that betain is used in the single treatment mode at early growth period according to each method of claim 6 to 10.
12. according to each method of claim 6 to 11, it is characterized in that the betain amount of application is about 0.2~20kg/ha, preferred amount of application is about 2~6kg/ha.
13. according to each product, especially the seed product of the prepared C-4 cereal of method of claim 6 to 12.
14. outside betain, execute the C-4 cereal and the seed thereof of processing.
15., it is characterized in that C-4 cereal is Chinese sorghum, corn and millet according to each purposes of claim 1 to 4.
CN95197917A 1995-06-09 1995-09-07 Improving yield of plants Pending CN1191466A (en)

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CN105409952A (en) * 2015-11-06 2016-03-23 浙江大学 Preparation for reducing damage to corn plants in cadmium pollution environments and method for applying preparation
CN110012906A (en) * 2019-05-06 2019-07-16 新疆盛德科技有限公司 Glycine betaine and tomatidine and anabasine class growth promotion preventing disease and pest mixed insecticide preparation
CN111556709A (en) * 2017-12-07 2020-08-18 丹斯塔发酵股份公司 Methods for improving seed germination and/or plant tolerance to environmental stress

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WO2017160914A1 (en) * 2016-03-15 2017-09-21 Valent Biosciences Corporation Methods to increase corn productivity
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CN105409952A (en) * 2015-11-06 2016-03-23 浙江大学 Preparation for reducing damage to corn plants in cadmium pollution environments and method for applying preparation
CN111556709A (en) * 2017-12-07 2020-08-18 丹斯塔发酵股份公司 Methods for improving seed germination and/or plant tolerance to environmental stress
CN111556709B (en) * 2017-12-07 2022-04-05 丹斯塔发酵股份公司 Methods for improving seed germination and/or plant tolerance to environmental stress
CN110012906A (en) * 2019-05-06 2019-07-16 新疆盛德科技有限公司 Glycine betaine and tomatidine and anabasine class growth promotion preventing disease and pest mixed insecticide preparation

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WO1996041532A1 (en) 1996-12-27
AU702833B2 (en) 1999-03-04
EP0831701A1 (en) 1998-04-01
FI952866A0 (en) 1995-06-09
FI98514C (en) 1997-07-10
FI952866A (en) 1996-12-10
BR9510603A (en) 2001-01-02

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