CN117209314A - Fish protein organic liquid fertilizer and production process thereof - Google Patents
Fish protein organic liquid fertilizer and production process thereof Download PDFInfo
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- CN117209314A CN117209314A CN202311193429.2A CN202311193429A CN117209314A CN 117209314 A CN117209314 A CN 117209314A CN 202311193429 A CN202311193429 A CN 202311193429A CN 117209314 A CN117209314 A CN 117209314A
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- 239000007788 liquid Substances 0.000 title claims abstract description 99
- 108010028690 Fish Proteins Proteins 0.000 title claims abstract description 77
- 239000003337 fertilizer Substances 0.000 title claims abstract description 58
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 230000008635 plant growth Effects 0.000 claims abstract description 32
- 230000017854 proteolysis Effects 0.000 claims abstract description 31
- 239000003102 growth factor Substances 0.000 claims abstract description 30
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- 239000008139 complexing agent Substances 0.000 claims abstract description 24
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- 229910052939 potassium sulfate Inorganic materials 0.000 claims abstract description 16
- 235000011151 potassium sulphates Nutrition 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 11
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 11
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 11
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- 238000000855 fermentation Methods 0.000 claims description 22
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- RFWGABANNQMHMZ-HYYSZPHDSA-N Oleuropein Chemical compound O([C@@H]1OC=C([C@H](C1=CC)CC(=O)OCCC=1C=C(O)C(O)=CC=1)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RFWGABANNQMHMZ-HYYSZPHDSA-N 0.000 claims description 2
- DDAQLPYLBPPPRV-UHFFFAOYSA-N [4-(hydroxymethyl)-2-oxo-1,3,2lambda5-dioxaphosphetan-2-yl] dihydrogen phosphate Chemical compound OCC1OP(=O)(OP(O)(O)=O)O1 DDAQLPYLBPPPRV-UHFFFAOYSA-N 0.000 claims description 2
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- RFWGABANNQMHMZ-CARRXEGNSA-N oleuropein Natural products COC(=O)C1=CO[C@@H](O[C@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2O)C(=CC)[C@H]1CC(=O)OCCc3ccc(O)c(O)c3 RFWGABANNQMHMZ-CARRXEGNSA-N 0.000 claims description 2
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 241000201912 Suaeda Species 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
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- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
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- 239000004475 Arginine Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
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- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
Abstract
The invention provides a fish protein organic liquid fertilizer and a production process thereof, wherein the fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 30-50 parts of fish protein degradation liquid, 18-22 parts of plant growth factors, 2-6 parts of potassium sulfate, 1-3 parts of monopotassium phosphate, 3-5 parts of urea and 1-3 parts of complexing agent; the fish protein degradation liquid, the plant growth factors and the trace elements are combined according to a specific proportion to cooperatively play the roles, so that the plant seed germination and seedling growth can be effectively promoted, soil nutrients can be activated and fertilized, the available nutrient content and the organic matter content in soil can be enhanced, the growth of crops can be facilitated, the germination rate of cabbage seeds reaches 89.3%, the root-cap ratio of seedlings is 0.51r/s through field experiments, meanwhile, pests can be effectively prevented and controlled, the average aphid prevention efficiency is 75.4-80.9%, the average white fly prevention efficiency is 70.9-75.9%, and the average cabbage caterpillar prevention efficiency is 80.8-83.8%.
Description
Technical Field
The invention relates to the technical field of production of biological organic fertilizers, in particular to a fish protein organic liquid fertilizer and a production process thereof.
Background
In recent years, with the rapid development of the aquatic product processing industry, a large amount of processing waste is discarded, so that the aquatic product processing waste has high protein content, contains rich phospholipids, minerals (calcium, phosphorus and the like) and other active ingredients, and can solve the problems of cadmium pollution and the like caused by common fertilizers and bring new economic and social benefits if the aquatic product processing waste is used for producing organic fertilizers. The utilization way of the fish wastes has close relation with the technical progress of the degradation process, the recycling method of the fish wastes comprises biological hydrolysis and chemical hydrolysis,
chemical hydrolysis includes acid hydrolysis and alkaline hydrolysis:
acid hydrolysis: the liquid acid hydrolysis of fish protein is usually carried out by using 6-8mol/L hydrochloric acid or 8mol/L sulfuric acid. The biggest feature of acid hydrolysis is that the hydrolysis is rapid and thorough, and can substantially hydrolyze all proteins to amino acids, and avoid racemization, but tryptophan is often destroyed. Wherein the hydrochloric acid used can be removed by evaporation, and if sulfuric acid is used, it can be removed by formation of a calcium sulfate precipitate. The disadvantage is that the black matter is easy to generate, the hydrolysate turns black, the filtering is difficult, and the equipment is easy to corrode.
Alkaline hydrolysis: sodium hydroxide is commonly used for degrading protein in alkaline hydrolysis, can avoid damage of tryptophan, serine and the like, does not generate putrescence, is clear in hydrolysate and easy to filter, but has the advantages that the hydrolysis speed is much slower than that of acid hydrolysis, all amino acids such as arginine, cystine and the like are damaged, the obtained amino acid is low in yield, the functionality of degradation products is poor, the nutritional value of hydrolyzed protein is not facilitated, and the same defects as acid hydrolysis are that equipment is easy to corrode.
The biological hydrolysis is to hydrolyze skin bonds of food proteins by utilizing enzymes, which can degrade the skin bonds by using enzymes existing in viscera or muscles of fish and by adding enzymes from other sources, but the current enzymolysis method is single, and the enzymolysis is only carried out by controlling the enzymolysis environment, so that the effective components are less, and the effect of the biological hydrolysis serving as an organic liquid fertilizer for promoting the growth of crops is poor; currently, microorganisms in the soil are greatly reduced, resulting in the generation of insect pests and diseases, thereby reducing crop yield.
Disclosure of Invention
In view of the above, the present invention provides a fish protein organic liquid fertilizer and a production process thereof, which solve the above problems.
The technical scheme of the invention is realized as follows: the fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 30-50 parts of fish protein degradation liquid, 18-22 parts of plant growth factors, 2-6 parts of potassium sulfate, 1-3 parts of monopotassium phosphate, 3-5 parts of urea and 1-3 parts of complexing agent; the plant growth factor is lactobacillus fermented extract of seaweed, suaeda salsa and Cactus flower, and the complexing agent is one or more of ethylenediamine tetraacetic acid, hydroxytyrosol, ethanolamine salt, hydroxyethylidene diphosphate and oleuropein.
Further, the fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 40 parts of fish protein degradation liquid, 20 parts of plant growth factors, 4 parts of potassium sulfate, 2 parts of monopotassium phosphate, 4 parts of urea and 2 parts of complexing agent.
Further, the plant growth factor is obtained by taking seaweed, common suaeda herb and cactus flower, mixing (8-12) and (2-7) and (25-68) according to the mass ratio, drying, crushing to obtain a mixed material, adding lactobacillus for fermentation for 12-20h, carrying out ultrasonic leaching at the fermentation temperature of 22-30 ℃ by using an ethanol solution with the mass concentration of 80-95%, and concentrating under reduced pressure after the fermentation is finished.
Further, the addition amount of the lactobacillus is 0.5-0.8 times of the mass of the mixture.
Further, the production process of the fish protein organic liquid fertilizer comprises the following steps:
s1, preparing fish paste slurry: soaking fish wastes in neutral salt solution, then soaking, washing and crushing the fish wastes with water, and carrying out superfine crushing, wherein the average diameter of micro powder in the superfine crushing is 0.1-2 mu m, and the cell crushing rate reaches 100%, so as to obtain fish slurry;
s2, pulse magnetic field enzymolysis: adding complex enzyme into the fish slurry for enzymolysis, and simultaneously introducing a pulse magnetic field, wherein the output voltage is 48-85V, the pulse width is 200-300ms, and the action frequency is 0.2-0.4Hz; the magnetic induction intensity generated by the pulse magnetic field is 0.5-3.2T, the enzymolysis temperature is 30-50 ℃, the pH is adjusted to 6-7, and the enzymolysis time is 4-8h, so as to obtain enzymolysis liquid;
s3, preparing a fish protein degradation liquid: extracting the enzymolysis liquid in the step S2 under the microwave condition, wherein the microwave power is 300-500W, the microwave frequency is 3000-10000MHz, the microwave temperature is 70-90 ℃, the microwave extraction is carried out for 30-60min, and the extracting liquid is filtered to obtain the fish protein degradation liquid;
s4, preparing an organic liquid fertilizer: adding complexing agent into the obtained fish protein degradation liquid, stirring for 8-15min, adding plant growth factor, potassium sulfate and urea respectively, and mixing and stirring at 60-80deg.C to obtain fish protein organic liquid fertilizer.
Further, the fish wastes comprise fish bones, fish scales, fish blood, fish steaks and fish viscera.
Further, the S1 neutral salt solution is sodium chloride solution, and the concentration is 0.05-0.15mol/L.
Further, the rotational speed of the ultra-fine crushing in the step S1 is 500-800r/min, and the time is 1.5-3.0h.
Further, the complex enzyme in the S2 is oxidoreductase, lyase, glucose oxidase and trypsin with the mass ratio of (0.9-1.2), (3.2-5.8), (6.8-9.7), (12.5-22.9).
Further, the addition amount of the complex enzyme is 0.1-0.3% of the mass of the fish mud material.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the fish protein degradation liquid, the plant growth factors and the trace elements are combined according to a specific proportion, so that the effects of the fish protein degradation liquid, the plant growth factors and the trace elements are synergistically exerted, the germination and seedling growth of plant seeds can be effectively promoted, soil nutrients can be activated and fertilized, the available nutrient content and the organic matter content in the soil can be enhanced, the growth of crops can be facilitated, and meanwhile, the pests can be effectively prevented and controlled.
The method comprises the steps of preparing a fish protein degradation liquid by effectively degrading rich protein content in fish wastes, and setting specific parameter conditions through enzymolysis and microwave extraction of a pulse magnetic field, so that a large amount of amino acids and small peptides can be produced in the degradation process, and the absorption and the utilization of crops are facilitated; the seaweed, the common suaeda herb and the cactus flower are adopted as raw materials, lactic acid bacteria are added according to the mass ratio for fermentation, various nutrient substances and natural plant active factors are extracted, the diversity and the biological activity of the seaweed, the common suaeda herb and the cactus flower extract are reserved, the seaweed, the common suaeda herb and the cactus flower extract are rich in various nutrient substances, trace elements and natural plant growth hormone which are required, the yield increasing effect on crops is achieved, and meanwhile, the plant diseases and insect pests can be prevented and controlled through the combination of the natural plant stimulators.
Detailed Description
In order to better understand the technical content of the present invention, the following provides specific examples to further illustrate the present invention.
The experimental methods used in the embodiment of the invention are conventional methods unless otherwise specified.
Materials, reagents, and the like used in the examples of the present invention are commercially available unless otherwise specified.
Example 1
The fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 30 parts of fish protein degradation liquid, 18 parts of plant growth factors, 2 parts of potassium sulfate, 1 part of monopotassium phosphate, 3 parts of urea and 1 part of complexing agent; the complexing agent is ethylenediamine tetraacetic acid;
the plant growth factor is obtained by mixing seaweed, suaeda salsa and cactus flowers according to a mass ratio of 8:2:25, drying, crushing to obtain a mixed material, adding lactobacillus for fermentation for 12 hours, wherein the adding amount of the lactobacillus is 0.5 times of the mass of the mixed material, the fermentation temperature is 22 ℃, and after the fermentation is finished, carrying out ultrasonic leaching by using an ethanol solution with the mass concentration of 80%, and concentrating under reduced pressure.
Example 2
The fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 50 parts of fish protein degradation liquid, 22 parts of plant growth factors, 6 parts of potassium sulfate, 3 parts of monopotassium phosphate, 5 parts of urea and 3 parts of complexing agent; the complexing agent is hydroxytyrosol;
the plant growth factor is obtained by mixing seaweed, suaeda salsa and cactus flowers according to a mass ratio of 12:7:68, drying, crushing to obtain a mixed material, adding lactobacillus for fermentation for 20 hours, wherein the adding amount of the lactobacillus is 0.8 times of the mass of the mixed material, the fermentation temperature is 30 ℃, and after the fermentation is finished, carrying out ultrasonic leaching by using an ethanol solution with a mass concentration of 95%, and concentrating under reduced pressure;
example 3
The fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 40 parts of fish protein degradation liquid, 20 parts of plant growth factors, 4 parts of potassium sulfate, 2 parts of monopotassium phosphate, 4 parts of urea and 2 parts of complexing agent; the complexing agent is ethylenediamine tetraacetic acid with the weight ratio of 1:1;
the plant growth factor is obtained by mixing seaweed, suaeda salsa and cactus flowers according to a mass ratio of 10:5:42, drying, crushing to obtain a mixed material, adding lactobacillus for fermentation for 16 hours, wherein the adding amount of the lactobacillus is 0.7 times of the mass of the mixed material, the fermentation temperature is 26 ℃, and after the fermentation is finished, carrying out ultrasonic leaching by using an ethanol solution with a mass concentration of 88%, and concentrating under reduced pressure;
the following preparation methods were employed for the above examples 1-3:
s1, preparing fish paste slurry: soaking fish wastes including fish bones, fish scales, fish blood, fish steaks and fish viscera in a sodium chloride solution with the concentration of 0.10mol/L, then soaking and washing with water, crushing, and carrying out superfine crushing for 2.2 hours at the rotating speed of 700r/min, wherein the average diameter of micro powder in the superfine crushing is 1.5 mu m, and the cell crushing rate reaches 100%, so as to obtain a fish slurry;
s2, pulse magnetic field enzymolysis: adding complex enzyme into the fish slurry for enzymolysis, wherein the addition amount of the complex enzyme is 0.2% of the mass of the fish slurry, the complex enzyme is oxidoreductase, lyase, glucose oxidase and trypsin with the mass ratio of 1.0:4.2:8.0:17.6, and simultaneously, a pulse magnetic field is introduced, the output voltage is 66V, the pulse width is 250ms, and the action frequency is 0.3Hz; the magnetic induction intensity which can be generated by the pulse magnetic field is 2.0T, the enzymolysis temperature is 40 ℃, the pH value is adjusted to 6, and the enzymolysis time is 6 hours, so that enzymolysis liquid is obtained;
s3, preparing a fish protein degradation liquid: extracting the enzymolysis liquid in the step S2 under the microwave condition, wherein the microwave power is 400W, the microwave frequency is 7000MHz, the microwave temperature is 80 ℃, the microwave extraction is carried out for 40min, and the extraction liquid is filtered to obtain the fish protein degradation liquid;
s4, preparing an organic liquid fertilizer: adding complexing agent into the obtained fish protein degradation liquid, stirring for 12min, adding plant growth factors, potassium sulfate and urea respectively, and mixing and stirring at 70 ℃ to obtain the fish protein organic liquid fertilizer.
Example 4
The fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 40 parts of fish protein degradation liquid, 20 parts of plant growth factors, 4 parts of potassium sulfate, 2 parts of monopotassium phosphate, 4 parts of urea and 2 parts of complexing agent; the complexing agent is ethylenediamine tetraacetic acid with the weight ratio of 1:1;
the plant growth factor is obtained by mixing seaweed, suaeda salsa and cactus flowers according to a mass ratio of 10:5:42, drying, crushing to obtain a mixed material, adding lactobacillus for fermentation for 16 hours, wherein the adding amount of the lactobacillus is 0.7 times of the mass of the mixed material, the fermentation temperature is 26 ℃, and after the fermentation is finished, carrying out ultrasonic leaching by using an ethanol solution with a mass concentration of 88%, and concentrating under reduced pressure;
the fish protein organic liquid fertilizer adopts the following preparation method:
s1, preparing fish paste slurry: soaking fish wastes including fish bones, fish scales, fish blood, fish steaks and fish viscera in a sodium chloride solution with the concentration of 0.05mol/L, then soaking and washing with water, crushing, and carrying out superfine crushing for 1.5 hours at the rotating speed of 500r/min, wherein the average diameter of micro powder in the superfine crushing is 0.1 mu m, and the cell crushing rate reaches 100%, so as to obtain a fish slurry;
s2, pulse magnetic field enzymolysis: adding complex enzyme into the fish slurry for enzymolysis, wherein the adding amount of the complex enzyme is 0.1-0.3% of the mass of the fish slurry, the complex enzyme is oxidoreductase, lyase, glucose oxidase and trypsin with the mass ratio of 0.9:3.2:6.8:12.5, and simultaneously, a pulse magnetic field is introduced, the output voltage is 48V, the pulse width is 200ms, and the action frequency is 0.2Hz; the magnetic induction intensity which can be generated by the pulse magnetic field is 0.5T, the enzymolysis temperature is 30 ℃, the pH value is adjusted to 6, and the enzymolysis time is 4 hours, so that enzymolysis liquid is obtained;
s3, preparing a fish protein degradation liquid: extracting the enzymolysis liquid in the step S2 under the microwave condition, wherein the microwave power is 300W, the microwave frequency is 3000MHz, the microwave temperature is 70 ℃, the microwave extraction is carried out for 30min, and the extraction liquid is filtered to obtain the fish protein degradation liquid;
s4, preparing an organic liquid fertilizer: adding complexing agent into the obtained fish protein degradation liquid, stirring for 8min, adding plant growth factors, potassium sulfate and urea respectively, and mixing and stirring at 60 ℃ to obtain the fish protein organic liquid fertilizer.
Example 5
The fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 40 parts of fish protein degradation liquid, 20 parts of plant growth factors, 4 parts of potassium sulfate, 2 parts of monopotassium phosphate, 4 parts of urea and 2 parts of complexing agent; the complexing agent is ethylenediamine tetraacetic acid with the weight ratio of 1:1;
the plant growth factor is obtained by mixing seaweed, suaeda salsa and cactus flowers according to a mass ratio of 10:5:42, drying, crushing to obtain a mixed material, adding lactobacillus for fermentation for 16 hours, wherein the adding amount of the lactobacillus is 0.7 times of the mass of the mixed material, the fermentation temperature is 26 ℃, and after the fermentation is finished, carrying out ultrasonic leaching by using an ethanol solution with a mass concentration of 88%, and concentrating under reduced pressure;
the fish protein organic liquid fertilizer adopts the following preparation method:
s1, preparing fish paste slurry: soaking fish wastes including fish bones, fish scales, fish blood, fish steaks and fish viscera in a sodium chloride solution with the concentration of 0.15mol/L, then soaking and washing with water, crushing, and carrying out superfine crushing for 3.0 hours at the rotating speed of 800r/min, wherein the average diameter of micro powder in the superfine crushing is 2 mu m, and the cell crushing rate reaches 100%, so as to obtain a fish slurry;
s2, pulse magnetic field enzymolysis: adding complex enzyme into the fish slurry for enzymolysis, wherein the adding amount of the complex enzyme is 0.1-0.3% of the mass of the fish slurry, the complex enzyme is oxidoreductase, lyase, glucose oxidase and trypsin with the mass ratio of 1.2:5.8:9.7:22.9, and simultaneously, a pulse magnetic field is introduced, the output voltage is 85V, the pulse width is 300ms, and the action frequency is 0.4Hz; the magnetic induction intensity which can be generated by the pulse magnetic field is 3.2T, the enzymolysis temperature is 50 ℃, the pH value is adjusted to 7, and the enzymolysis time is 8 hours, so that enzymolysis liquid is obtained;
s3, preparing a fish protein degradation liquid: extracting the enzymolysis liquid in the step S2 under the microwave condition, wherein the microwave power is 500W, the microwave frequency is 10000MHz, the microwave temperature is 90 ℃, the microwave extraction is carried out for 60min, and the extraction liquid is filtered to obtain the fish protein degradation liquid;
s4, preparing an organic liquid fertilizer: adding complexing agent into the obtained fish protein degradation liquid, stirring for 15min, adding plant growth factors, potassium sulfate and urea respectively, and mixing and stirring at 80 ℃ to obtain the fish protein organic liquid fertilizer.
Comparative example 1
The comparative example differs from example 3 in that a fish protein organic liquid fertilizer comprises the following raw materials in parts by weight: 20 parts of fish protein degradation liquid, 15 parts of plant growth factors, 8 parts of potassium sulfate, 8 parts of monopotassium phosphate, 1 part of urea and 5 parts of complexing agent.
Comparative example 2
The difference between this comparative example and example 3 is that a fish protein organic liquid fertilizer is not added with plant growth factors.
Comparative example 3
This comparative example differs from example 3 in that a fish protein organic liquid fertilizer is not added with complexing agent.
Comparative example 4
The difference between the comparative example and the example 3 is that in the production process of the fish protein organic liquid fertilizer, the enzymolysis of S2 does not introduce a pulse magnetic field.
Comparative example 5
The comparative example is different from example 3 in that the composite enzyme is oxidoreductase, lyase, glucose oxidase and trypsin with a mass ratio of 0.2:1.3:10.3:5.2.
1. Effect verification
1. Setting 11 areas, respectively planting Chinese cabbage, diluting the organic liquid fertilizer prepared in the examples 1-5 and the comparative examples 1-5 and the commercial fish protein organic liquid fertilizer by 500 times, soaking white vegetable seeds at 25 ℃ for 24 hours; planting Chinese cabbage in soil, applying fertilizer at the growth stage for 4 weeks, applying fertilizer with daily fertilizer amount of 0.3 kg/plant, 0.6 kg/plant, 0.5 kg/plant, 2.25 kg/plant, determining germination rate and root cap ratio, determining soil nutrient and disinfestation effect,
germination rate = number of germinated seeds/number of test seeds x 100;
the measurement results were as follows:
the results show that: the fish protein organic liquid fertilizer prepared by the invention is used for soaking cabbage seeds and has higher germination rate, the root cap ratio is good, the root system development of seedlings is effectively promoted by combining the fish protein degradation liquid, plant growth factors and trace elements, the root cap ratio of the cabbage is improved, and compared with the fish protein organic liquid fertilizer sold on the market, the soil organic matters and available nutrients of the fish protein organic liquid fertilizer are increased by applying the fish protein organic liquid fertilizer disclosed by the invention, so that the content of available nutrients in the soil can be obviously increased by the fish protein organic liquid fertilizer, the fish protein organic liquid fertilizer is favorable for crop absorption, and the organic matters rich in the fish protein organic liquid fertilizer are quickly converted into the soil, so that the growth of the seedlings is positively promoted.
2. Insect pest prevention efficiency:
the investigation method comprises the following steps: the insect population base number is investigated before fertilization, 5 points are sampled on the diagonal line of each cell, 10 Chinese cabbage plants are fixedly investigated at each point, and marking is carried out. The number of living insects (aphids, white flies and cabbage caterpillars) on 50 Chinese cabbage plants is checked.
The investigation and calculation of the control effect were carried out in the same way at 14d after the application.
The results show that after the fish protein organic liquid fertilizer is applied for 14 days, the average prevention efficiency of aphids is 75.4-80.9%, the average prevention efficiency of white flies is 70.9-75.9%, and the average prevention efficiency of cabbage caterpillars is 80.8-83.8%, which indicates that the fish protein organic liquid fertilizer has a certain prevention effect on insect pests and lasts for 14 days.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (10)
1. A fish protein organic liquid fertilizer is characterized in that: the material comprises the following raw materials in parts by weight: 30-50 parts of fish protein degradation liquid, 18-22 parts of plant growth factors, 2-6 parts of potassium sulfate, 1-3 parts of monopotassium phosphate, 3-5 parts of urea and 1-3 parts of complexing agent; the plant growth factor is lactobacillus fermented extract of seaweed, suaeda salsa and Cactus flower, and the complexing agent is one or more of ethylenediamine tetraacetic acid, hydroxytyrosol, ethanolamine salt, hydroxyethylidene diphosphate and oleuropein.
2. A fish protein organic liquid fertilizer as claimed in claim 1, wherein: the material comprises the following raw materials in parts by weight: 40 parts of fish protein degradation liquid, 20 parts of plant growth factors, 4 parts of potassium sulfate, 2 parts of monopotassium phosphate, 4 parts of urea and 2 parts of complexing agent.
3. A fish protein organic liquid fertilizer as claimed in claim 1, wherein: the plant growth factor is obtained by mixing seaweed, suaeda salsa and cactus flowers according to the mass ratio of (8-12) (2-7) (25-68), drying, crushing to obtain a mixed material, adding lactobacillus for fermentation for 12-20h at the fermentation temperature of 22-30 ℃, performing ultrasonic leaching by using an ethanol solution with the mass concentration of 80-95% after the fermentation is finished, and concentrating under reduced pressure.
4. A fish protein organic liquid fertilizer as claimed in claim 3, wherein: the addition amount of the lactic acid bacteria is 0.5-0.8 times of the mass of the mixture.
5. The process for producing the fish protein organic liquid fertilizer according to claim 1, which is characterized in that: the method comprises the following steps:
s1, preparing fish paste slurry: soaking fish wastes in neutral salt solution, then soaking, washing and crushing the fish wastes with water, and carrying out superfine crushing, wherein the average diameter of micro powder in the superfine crushing is 0.1-2 mu m, and the cell crushing rate reaches 100%, so as to obtain fish slurry;
s2, pulse magnetic field enzymolysis: adding complex enzyme into the fish slurry for enzymolysis, and simultaneously introducing a pulse magnetic field, wherein the output voltage is 48-85V, the pulse width is 200-300ms, and the action frequency is 0.2-0.4Hz; the magnetic induction intensity generated by the pulse magnetic field is 0.5-3.2T, the enzymolysis temperature is 30-50 ℃, the pH is adjusted to 6-7, and the enzymolysis time is 4-8h, so as to obtain enzymolysis liquid;
s3, preparing a fish protein degradation liquid: extracting the enzymolysis liquid in the step S2 under the microwave condition, wherein the microwave power is 300-500W, the microwave frequency is 3000-10000MHz, the microwave temperature is 70-90 ℃, the microwave extraction is carried out for 30-60min, and the extracting liquid is filtered to obtain the fish protein degradation liquid;
s4, preparing an organic liquid fertilizer: adding complexing agent into the obtained fish protein degradation liquid, stirring for 8-15min, adding plant growth factor, potassium sulfate and urea respectively, and mixing and stirring at 60-80deg.C to obtain fish protein organic liquid fertilizer.
6. The production process of the fish protein organic liquid fertilizer according to claim 5, which is characterized in that: the fish wastes comprise fish bones, fish scales, fish blood, fish steaks and fish viscera.
7. The production process of the fish protein organic liquid fertilizer according to claim 5, which is characterized in that: the S1 neutral salt solution is sodium chloride solution with the concentration of 0.05-0.15mol/L.
8. The production process of the fish protein organic liquid fertilizer according to claim 5, which is characterized in that: the rotational speed of the superfine crushing in the step S1 is 500-800r/min, and the time is 1.5-3.0h.
9. The production process of the fish protein organic liquid fertilizer according to claim 5, which is characterized in that: the complex enzyme in the S2 is oxidoreductase, lyase, glucose oxidase and trypsin in a mass ratio of (0.9-1.2), (3.2-5.8), (6.8-9.7) and (12.5-22.9).
10. The production process of the fish protein organic liquid fertilizer according to claim 5, which is characterized in that: the addition amount of the complex enzyme is 0.1-0.3% of the mass of the fish mud material.
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