CN111454105A - Fertilizer synergist containing fish protein oligopeptide and preparation method thereof - Google Patents
Fertilizer synergist containing fish protein oligopeptide and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a fertilizer synergist containing fish protein oligopeptide and a preparation method thereof, the fertilizer synergist comprises 60-80 parts of fish protein powder, 5-10 parts of carbon source, 3-5 parts of nitrogen source, 0.4-0.6 part of soil loosening agent, 1.2-1.5 parts of medium trace element and 0.5-1.2 parts of crop growth regulator, the fish protein mixed peptide powder, the carbon source, the nitrogen source, the soil loosening agent, the medium trace element and the crop growth regulator are taken according to the formula proportion, water is added and stirred uniformly to prepare a mixed solution, the obtained solution is subjected to filter pressing by a filter press, 2-5 parts of boric acid is added into the obtained filtrate, hydrochloric acid solution containing 3-5% acetic acid is used for regulating the pH of the solution to 7.5-8.5, evaporation concentration and split charging are carried out, the fish protein oligopeptide prepared by the invention, the amino acid after effective activation becomes high-efficiency bioactive substance, can effectively stimulate the growth of crop roots, and has the characteristics of enhancing the soil fertility, reducing the soil hardening and improving the soil microenvironment.
Description
Technical Field
The invention belongs to the technical field of fertilizer synergists, and particularly relates to a fertilizer synergist containing fish protein oligopeptides and a preparation method thereof.
Background
The amino acid fertilizer can promote photosynthesis and chlorophyll formation, and has obvious effects of promoting and activating the physiological and biochemical processes of oxide activity, enzyme activity, seed germination, nutrient absorption, root growth and development and the like. In particular, its affinity for plants is not comparable to that of any other substance. The efficacy of the amino acid fertilizer integrates the long-acting effect of the organic fertilizer, the quick-acting effect of the fertilizer, the stable effect of the biological fertilizer and the synergistic effect of the micro-fertilizer. The activated amino acid becomes a high-efficiency bioactive substance, has stimulation effect on the growth and development of crops and physiological metabolism in vivo, and has obvious stimulation effect on various crops by adopting the modes of seed soaking, root dipping, wine spraying, irrigating, base fertilizer making and the like according to certain concentration. The comprehensive expression is to promote the development of root system and to have good influence on the yield and quality of crops. Meanwhile, the coastline in China is 1.8 kilometers in length, and is located in the fourth place of the world, so that abundant aquatic resources are provided, the organisms in the sea are various and large in quantity, and meanwhile, the lakes and rivers in China are numerous, and huge fish resources are reserved in numerous rivers and lakes. In the industrialized era, the quantity of leftovers of fish, shrimp and the like is huge, so that the research on how to utilize the products of fish, shrimp and the like and the leftovers thereof makes a contribution to the production and income increase of agriculture has very important strategic significance, and simultaneously conforms to the green development concept of China.
Disclosure of Invention
Aiming at various defects in the prior art, the fertilizer synergist containing the fish protein oligopeptide is provided, so that leftovers of fishes, shrimps and the like can be effectively utilized, and the problems of soil hardening, salinization and the like can be effectively improved.
In order to achieve the purpose, the invention provides the following technical scheme:
a fertilizer synergist containing fish protein oligopeptide comprises 60-80 parts of fish protein powder, 0.4-0.6 part of soil loosening agent, 1.2-1.5 parts of medium trace elements and 0.5-1.2 parts of crop growth regulator.
Preferably, the soil loosening agent is 14-26 parts of decomposed organic matters, 0.6-8 parts of wood rotting fungus hyphae, 32-40 parts of plant ash and 6-9 parts of calcium organophosphate.
Preferably, the medium trace elements comprise chelated elements consisting of 0.01-0.03% of EDTA chelated copper, 0.3-0.5% of EDTA chelated iron, 0.06-0.08% of EDTA chelated manganese, 0.7-0.9% of EDTA chelated zinc, 0.2-0.4% of EDTA chelated magnesium and 0.7-0.9% of EDTA chelated calcium in percentage by weight.
Preferably, the crop growth agent comprises sodium selenite, gibberellin, and cytokinin, and the ratio of sodium selenite: gibberellin: cytokinins are 0.3-0.6: 0.1-0.3: 0.8-1.2.
Preferably, the fish protein powder is prepared according to the following steps: selecting fish meat from fish leftovers, mincing with a stirrer to obtain fish meat slurry, degreasing the fish meat slurry with ethanol solution with volume concentration of more than or equal to 95%, and then sequentially air-drying, freeze-drying and crushing to obtain crude fish protein powder.
Preferably, the prepared crude fish protein powder is processed to prepare the fish protein mixed peptide powder according to the following steps:
s1, taking a proper amount of crude fish protein powder, adding deionized water, and stirring and mixing uniformly to obtain slurry;
s2, adding phospholipase into the slurry for enzymolysis, inactivating enzyme after the enzymolysis is finished, and removing fat through supercritical carbon dioxide extraction to obtain degreased slurry;
s3, adding the fishy smell removing liquid into the degreased slurry, stirring for 10-20min, standing for 2-3h, dialyzing to obtain a retentate to obtain fishy smell removing slurry;
s4, adding compound protease into the fishy smell removed slurry, placing the fishy smell removed slurry in a constant-temperature oscillator at 35-45 ℃ for continuous enzymolysis for 3-4h, cooling to room temperature, adding a buffer solution to adjust the pH value of the solution to 6.5-7.5, and obtaining the fish protein mixed peptide solution.
Preferably, the deodorization liquid comprises the following components in parts by weight: 60-90 parts of ethanol, 20-30 parts of acetic acid, 8-15 parts of baking soda, 6-8 parts of citric acid and 5-11 parts of ginger juice.
Preferably, the compound protease comprises a composition of trypsin, chymotrypsin, neutral protease, alkaline protease, papain and flavourzyme, and the mass ratio of the trypsin to the chymotrypsin to the neutral protease to the alkaline protease to the papain to the flavourzyme is 2-3:1-2.5:1.1-2.1:0.8-1.5:0.9-1.4: 1.1-1.5.
Preferably, the buffer is a citrate buffer.
In addition, the invention also provides a method for preparing a fertilizer synergist containing the fish protein oligopeptide, which comprises the following steps: the method comprises the following steps: taking the fish protein mixed peptide solution, the soil loosening agent, the medium trace elements and the crop growth regulator according to the formula ratio, adding water, and uniformly stirring to obtain a mixed solution.
Step two: and (3) performing filter pressing on the solution obtained in the step one through a filter press, adding 2-5 parts of boric acid into the obtained filtrate, adjusting the pH of the solution to 7.5-8.5 by using a hydrochloric acid solution containing 3-5% of acetic acid, performing evaporation concentration until the concentration of a fixed object is more than or equal to 8%, and subpackaging.
The invention has the beneficial effects that:
1. the fertilizer synergist contains fish protein oligopeptide, and amino acid after effective activation of the fish protein oligopeptide becomes a high-efficiency bioactive substance, so that the growth of crop root systems can be effectively stimulated.
2. The soil loosening agent is contained, so that the soil loosening degree can be effectively increased, the soil hardening is prevented, and the growth of plant roots is facilitated.
3. The invention adds medium trace elements and boric acid in the raw material formula, can effectively balance the content of each element in soil, promotes the growth of root systems, and can prevent diseases of crops caused by the lack of trace elements.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
a fertilizer synergist containing fish protein oligopeptide comprises 60 parts of fish protein powder, 0.4 part of a soil loosening agent, 1.2 parts of medium trace elements and 0.5 part of a crop growth regulator.
The soil loosening agent comprises 14 parts of decomposed organic matters, 0.6 part of wood rotting fungi hyphae, 32 parts of plant ash and 6 parts of calcium organophosphate. The medium trace elements comprise chelated elements consisting of 0.01 percent of EDTA chelated copper, 0.3 percent of EDTA chelated iron, 0.06 percent of EDTA chelated manganese, 0.7 percent of EDTA chelated zinc, 0.2 percent of EDTA chelated magnesium and 0.7 percent of EDTA chelated calcium.
The crop growth agent comprises sodium selenite, gibberellin and cytokinin, and the ratio of sodium selenite: gibberellin: cytokinins were 0.3: 0.1: 0.8.
preparing fish protein powder according to the following steps: selecting fish meat from fish leftovers, mincing the fish meat with a stirrer to obtain fish meat slurry, adding an ethanol solution with the same volume as the fish meat slurry into the obtained fish meat slurry, degreasing the fish meat slurry, wherein the volume concentration of the ethanol solution is more than or equal to 95%, and then sequentially carrying out air drying, freeze drying and crushing to obtain crude fish protein powder.
Preparing the prepared crude fish protein powder into fish protein mixed peptide powder according to the following steps:
taking a proper amount of crude fish protein powder, adding deionized water with the volume of 1 time of that of the crude fish protein powder, and stirring and mixing uniformly to obtain slurry; adding phospholipase into the slurry for enzymolysis, wherein the phospholipase is 0.5-1.5% of the volume of the slurry, and inactivating enzyme after enzymolysis, wherein the phospholipase is phospholipase A1, phospholipase A2, phospholipase B and phospholipase C, and the ratio of the phospholipase A1 to the phospholipase A2 to the phospholipase B to the phospholipase C is 1: 1: 1: and 1, removing fat by supercritical carbon dioxide extraction to obtain degreased slurry. Adding fishy smell removing solution with volume of 0.5% of the defatted slurry into the defatted slurry after fat extraction, stirring at 600r/s for 10min, standing for 2 hr, and dialyzing to obtain retained solution. Adding compound protease into the fishy smell removed slurry, placing the fishy smell removed slurry in a constant-temperature oscillator at 35 ℃ for continuous enzymolysis for 3h, cooling to room temperature, adding a buffer solution to adjust the pH value of the solution to 6.5-7.5, and obtaining the fish protein mixed peptide solution.
The buffer solution used in this example was a citric acid buffer solution.
The fishy smell removing liquid comprises the following components in parts by weight: 60 parts of ethanol, 20 parts of acetic acid, 8 parts of baking soda, 6 parts of citric acid and 5 parts of ginger juice, wherein the preparation method of the fishy smell removing liquid comprises the steps of adding the ginger juice into an ethanol solution, stirring for 10min, then sequentially adding the acetic acid, the citric acid and the baking soda, and stirring for 5 min.
The compound protease comprises a composition of trypsin, chymotrypsin, neutral protease, alkaline protease, papain and flavourzyme, and the mass ratio of the trypsin to the chymotrypsin to the neutral protease to the alkaline protease to the papain to the flavourzyme is 2: 1: 1.1: 0.8: 0.9: 1.1. the buffer solution is a citric acid buffer solution.
Taking the fish protein mixed peptide solution, the soil loosening agent, the medium trace elements and the crop growth regulator according to the formula ratio, adding 2 times of water by volume, and uniformly stirring to prepare a mixed solution. And (3) carrying out filter pressing on the obtained mixed solution through a filter press, adding 2 parts of boric acid into the obtained filtrate, adjusting the pH of the solution to 7.5-8.5 by using hydrochloric acid solution containing 3-5% of acetic acid, evaporating and concentrating until the concentration of a fixed object is more than or equal to 8%, and subpackaging.
Example two
A fertilizer synergist containing fish protein oligopeptide comprises 80 parts of fish protein powder, 0.6 part of soil loosening agent, 1.5 parts of medium trace elements and 1.2 parts of crop growth regulator.
The soil loosening agent comprises 26 parts of decomposed organic matters, 8 parts of wood rotting fungi hyphae, 40 parts of plant ash and 9 parts of calcium organophosphate. The medium trace elements comprise chelated elements consisting of 0.03 percent of EDTA chelated copper, 0.5 percent of EDTA chelated iron, 0.08 percent of EDTA chelated manganese, 0.9 percent of EDTA chelated zinc, 0.4 percent of EDTA chelated magnesium and 0.9 percent of EDTA chelated calcium.
The crop growth agent comprises sodium selenite, gibberellin and cytokinin, and the ratio of sodium selenite: gibberellin: cytokinins were 0.6: 0.3: 1.2.
preparing fish protein powder according to the following steps: selecting fish meat from fish leftovers, mincing the fish meat with a stirrer to obtain fish meat slurry, adding an ethanol solution with the same volume as the fish meat slurry into the obtained fish meat slurry, degreasing the fish meat slurry, wherein the volume concentration of the ethanol solution is more than or equal to 95%, and then sequentially carrying out air drying, freeze drying and crushing to obtain crude fish protein powder.
Preparing the prepared crude fish protein powder into fish protein mixed peptide powder according to the following steps:
taking a proper amount of crude fish protein powder, adding deionized water with the volume of 1 time of that of the crude fish protein powder, and stirring and mixing uniformly to obtain slurry; adding phospholipase into the slurry for enzymolysis, wherein the volume of the phospholipase is 1.5% of the volume of the slurry, and inactivating enzymes after enzymolysis, wherein the phospholipase is phospholipase A1, phospholipase A2, phospholipase B and phospholipase C, and the ratio of the phospholipase A1 to the phospholipase A2 to the phospholipase B to the phospholipase C is 1: 1: 1: and 1, removing fat by supercritical carbon dioxide extraction to obtain degreased slurry. Adding fishy smell removing solution with volume of 0.5% of the defatted slurry into the defatted slurry after fat extraction, stirring at 600r/s for 20min, standing for 3 hr, and dialyzing to obtain retained solution. Adding compound protease into the fishy smell removed slurry, placing the fishy smell removed slurry in a constant temperature oscillator at 35-45 ℃ for continuous enzymolysis for 4h, cooling to room temperature, adding a buffer solution to adjust the pH value of the solution to 6.5-7.5, and obtaining the fish protein mixed peptide solution. The buffer solution used in this example was a citric acid buffer solution.
The fishy smell removing liquid comprises the following components in parts by weight: 90 parts of ethanol, 30 parts of acetic acid, 15 parts of baking soda, 8 parts of citric acid and 11 parts of ginger juice, wherein the preparation method of the fishy smell removing liquid comprises the steps of adding the ginger juice into an ethanol solution, stirring for 25min, sequentially adding the acetic acid, the citric acid and the baking soda, and stirring for 10 min.
The compound protease comprises a composition of trypsin, chymotrypsin, neutral protease, alkaline protease, papain and flavourzyme, and the mass ratio of the trypsin to the chymotrypsin to the neutral protease to the alkaline protease to the papain to the flavourzyme is 3: 2.5: 2.1: 1.5: 1.4: 1.5. the buffer solution is a citric acid buffer solution.
Taking the fish protein mixed peptide solution, the soil loosening agent, the medium trace elements and the crop growth regulator according to the formula ratio, adding 3 times of water by volume, and uniformly stirring to prepare a mixed solution. And (3) carrying out filter pressing on the obtained mixed solution through a filter press, adding 5 parts of boric acid into the obtained filtrate, adjusting the pH of the solution to 7.5-8.5 by using hydrochloric acid solution containing 3-5% of acetic acid, evaporating and concentrating until the concentration of a fixed object is more than or equal to 8%, and subpackaging.
EXAMPLE III
A fertilizer synergist containing fish protein oligopeptide comprises 70 parts of fish protein powder, 0.5 part of a soil loosening agent, 1.4 parts of medium trace elements and 0.8 part of a crop growth regulator.
The soil loosening agent comprises 20 parts of decayed organic matters, 4.8 parts of wood rotting fungi hyphae, 35 parts of plant ash and 8 parts of organic calcium phosphate. The medium trace elements comprise chelated elements consisting of 0.02 percent of EDTA chelated copper, 0.4 percent of EDTA chelated iron, 0.07 percent of EDTA chelated manganese, 0.8 percent of EDTA chelated zinc, 0.3 percent of EDTA chelated magnesium and 0.8 percent of EDTA chelated calcium.
The crop growth agent comprises sodium selenite, gibberellin and cytokinin, and the ratio of sodium selenite: gibberellin: cytokinins were 0.4: 0.2: 1.
preparing fish protein powder according to the following steps: selecting fish meat from fish leftovers, mincing the fish meat with a stirrer to obtain fish meat slurry, adding an ethanol solution with the same volume as the fish meat slurry into the obtained fish meat slurry, degreasing the fish meat slurry, wherein the volume concentration of the ethanol solution is more than or equal to 95%, and then sequentially carrying out air drying, freeze drying and crushing to obtain crude fish protein powder.
Preparing the prepared crude fish protein powder into fish protein mixed peptide powder according to the following steps:
taking a proper amount of crude fish protein powder, adding deionized water with the volume of 1 time of that of the crude fish protein powder, and stirring and mixing uniformly to obtain slurry; adding phospholipase into the slurry for enzymolysis, wherein the phospholipase is 1% of the volume of the slurry, and inactivating the enzyme after the enzymolysis is finished, wherein the phospholipase is phospholipase A1, phospholipase A2, phospholipase B and phospholipase C, and the ratio of the phospholipase A1 to the phospholipase A2 to the phospholipase B to the phospholipase C is 1: 1: 1: and 1, removing fat by supercritical carbon dioxide extraction to obtain degreased slurry. Adding fishy smell removing solution with volume of 0.5% of the defatted slurry into the defatted slurry after fat extraction, stirring at 600r/s for 20min, standing for 2.5 hr, and dialyzing to obtain the residual solution. Adding compound protease into the fishy smell removed slurry, placing the fishy smell removed slurry in a constant temperature oscillator at 35-45 ℃ for continuous enzymolysis for 3.5h, cooling to room temperature, adding a buffer solution to adjust the pH value of the solution to 6.5-7.5, and obtaining the fish protein mixed peptide solution. The buffer solution used in this example was a citric acid buffer solution.
The fishy smell removing liquid comprises the following components in parts by weight: 80 parts of ethanol, 25 parts of acetic acid, 10 parts of baking soda, 7 parts of citric acid and 9 parts of ginger juice, wherein the preparation method of the fishy smell removing liquid comprises the steps of adding the ginger juice into an ethanol solution, stirring for 20min, sequentially adding the acetic acid, the citric acid and the baking soda, and stirring for 8 min.
The compound protease comprises a composition of trypsin, chymotrypsin, neutral protease, alkaline protease, papain and flavourzyme, and the mass ratio of the trypsin to the chymotrypsin to the neutral protease to the alkaline protease to the papain to the flavourzyme is 1.5: 1.1: 2: 1.3: 0.2: 1.4. the buffer solution is a citric acid buffer solution.
Taking the fish protein mixed peptide solution, the soil loosening agent, the medium trace elements and the crop growth regulator according to the formula ratio, adding 2.5 times of water by volume, and uniformly stirring to prepare the mixed solution. And (3) carrying out filter pressing on the obtained mixed solution through a filter press, adding 4 parts of boric acid into the obtained filtrate, adjusting the pH of the solution to 7.5-8.5 by using hydrochloric acid solution containing 3-5% of acetic acid, evaporating and concentrating until the concentration of a fixed object is more than or equal to 8%, and subpackaging.
Control group B: comprises 0.4 part of soil loosening agent, 1.2 parts of medium trace elements and 0.5 part of crop growth regulator.
The soil loosening agent comprises 14 parts of decomposed organic matters, 0.6 part of wood rotting fungi hyphae, 32 parts of plant ash and 6 parts of calcium organophosphate. The medium trace elements comprise chelated elements consisting of 0.01 percent of EDTA chelated copper, 0.3 percent of EDTA chelated iron, 0.06 percent of EDTA chelated manganese, 0.7 percent of EDTA chelated zinc, 0.2 percent of EDTA chelated magnesium and 0.7 percent of EDTA chelated calcium. The crop growth agent comprises sodium selenite, gibberellin and cytokinin, and the ratio of sodium selenite: gibberellin: cytokinins were 0.3: 0.1: 0.8.
the soil loosening agent, the medium trace elements and the crop growth regulator are taken according to the formula ratio, 2 times of water is added, and the mixture is stirred uniformly to prepare the mixed solution. And (3) carrying out filter pressing on the obtained mixed solution through a filter press, adding 2 parts of boric acid into the obtained filtrate, adjusting the pH of the solution to 7.5-8.5 by using hydrochloric acid solution containing 3-5% of acetic acid, evaporating and concentrating until the concentration of a fixed object is more than or equal to 8%, and subpackaging.
First experimental example: the influence of the fertilizer synergist containing the fish protein oligopeptide on the activity of the root system of the wheat in the seedling stage
The sand culture test is utilized to research the influence of the fertilizer synergist containing the fish protein oligopeptide on the activity of the root system of the wheat in the seedling stage, wherein the fertilizer synergist is suitable for a drip irrigation and micro-sprinkling irrigation system.
The experiment was conducted by repeating 6 times, while a blank Control (CK), a control group B and 5 treatments of the fertilizer synergist containing fish protein oligopeptides of the present invention (A-1, A-2, A-3), wherein A-1, A-2, A-3 were the fertilizer synergist containing fish protein oligopeptides prepared in examples 1, 2, 3, respectively.
The test method is as follows:
firstly, quartz sand is cleaned by clear water, put into a stainless steel tray, keep certain humidity, put wheat seeds into the tray, covered with wet cloth, and cultured at the temperature of 25 ℃. When the wheat sprouts to 3-5cm, the wheat is taken out and put into a culture cup filled with quartz sand, 25ml of fertilizer synergist containing 0.2 weight percent of fish protein oligopeptide is poured every day, and 25ml of water is poured into CK. When the wheat grows to about 20cm, taking out the root system, washing with clear water, and measuring the activity of the wheat root and the pollen by adopting a TTC method (the reference document: Zhangxiong, the activity of the wheat root and the pollen by using a TTC method (red tetrazole) and the application thereof, and the root system activity of the corn is measured by adopting a plant physiology communication, 3 rd phase 1982).
The test results are shown in Table 1 below.
TABLE 1 wheat root vigor (mg/g. h, TTC method)
Compared with the conventional control, A-1, A-2 and A-3 are respectively higher by 30.9 percent, 29.8 percent and 39.2 percent than the blank control and respectively higher by 9.23 percent, 8.32 percent and 16.14 percent than the control group B, and the fertilizer synergist containing the fish protein oligopeptide can obviously improve the root activity of wheat at the seedling stage.
Test example 2: the influence of the fertilizer synergist containing the fish protein oligopeptide on the corn yield and the yield forming factors is avoided.
The test was conducted by setting 5 treatments of CK (no fertilizer application), control group B and the fertilizer synergist containing fish protein oligopeptide of the present invention (A-1, A-2, A-3), wherein A-1, A-2, A-3 were the fertilizer synergist containing fish protein oligopeptide prepared in examples 1, 2, 3, respectively.
A fertilizing mode: control groups B and A-1, A-2, A-3 were dissolved in water and then irrigated at leaf stage 3 and leaf stage 9 of corn, half each time, for a total of 5 treatments, repeated 8 times, and randomized. Leaving 30cm of the upper layer of the soil column as a soil-fertilizer mixing layer, applying the tested fertilizer as a base fertilizer, and adding calcium superphosphate (P)2O516%) 12.50g of potassium chloride (K) was applied per column2O60%) was applied 3.33g per column, one-time application was made during column packing. 5 corn seeds are sown in each corn earth pillar, and after seedling emergence, one corn seed with the best growth vigor is planted, the plant spacing is 40cm, and the row spacing is 80 cm.
Table 2: corn yield, composition factor and apparent utilization rate of nitrogen fertilizer
| Treatment of | Yield (g/strain) | Grain number per ear | Weight of hundred grains | Yield increase (%) |
| CK | 25.72 | 138 | 18.51 | -- |
| Control group B | 42.86 | 252 | 18.84 | -- |
| A-1 | 56.32 | 291 | 19.35 | 31.40 |
| A-2 | 57.15 | 301 | 19.69 | 33.34 |
| A-3 | 57.02 | 296 | 19.43 | 33.04 |
Compared with the control group B, the fertilizer synergist containing the fish protein oligopeptides can remarkably increase the yield and the grain number per ear of corn by 31.40%, 33.34%, 33.04%, 15.48%, 19.44% and 17.46% respectively, and the weight of one hundred grains is increased by 2.71%, 4.51% and 3.13%. Therefore, under the condition of simulating a drip irrigation test, the invention is suitable for a drip irrigation and micro-sprinkling irrigation system and can obviously increase the yield of corn kernels.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. A fertilizer synergist containing fish protein oligopeptides is characterized by comprising 60-80 parts of fish protein powder, 0.4-0.6 part of soil loosening agent, 1.2-1.5 parts of medium trace elements and 0.5-1.2 parts of crop growth regulator.
2. The fertilizer synergist according to claim 1, wherein the soil bulking agent comprises 14-26 parts of decomposed organic matter, 0.6-8 parts of wood rotting fungi hyphae, 32-40 parts of plant ash and 6-9 parts of calcium organophosphate.
3. The fertilizer synergist according to claim 1, wherein said medium trace elements comprise chelated elements consisting of 0.01-0.03% of copper EDTA chelate, 0.3-0.5% of iron EDTA chelate, 0.06-0.08% of manganese EDTA chelate, 0.7-0.9% of zinc EDTA chelate, 0.2-0.4% of magnesium EDTA chelate, and 0.7-0.9% of calcium EDTA chelate.
4. The fertilizer synergist of claim 1, wherein the crop growth agent comprises sodium selenite, gibberellin, and cytokinin, and the ratio of sodium selenite: gibberellin: cytokinins are 0.3-0.6: 0.1-0.3: 0.8-1.2.
5. The fertilizer synergist according to claim 1, wherein the fish protein powder is prepared by the following steps: selecting fish meat from fish leftovers, mincing with a stirrer to obtain fish meat slurry, degreasing the fish meat slurry with ethanol solution with volume concentration of more than or equal to 95%, and then sequentially air-drying, freeze-drying and crushing to obtain crude fish protein powder.
6. The fertilizer synergist according to claim 5, wherein the obtained crude fish protein powder is processed to prepare fish protein mixed peptide powder according to the following steps:
s1, taking a proper amount of crude fish protein powder, adding deionized water, and stirring and mixing uniformly to obtain slurry;
s2, adding phospholipase into the slurry for enzymolysis, inactivating enzyme after the enzymolysis is finished, and removing fat through supercritical carbon dioxide extraction to obtain degreased slurry;
s3, adding the fishy smell removing liquid into the degreased slurry, stirring for 10-20min, standing for 2-3h, dialyzing to obtain a retentate to obtain fishy smell removing slurry;
s4, adding compound protease into the fishy smell removed slurry, placing the fishy smell removed slurry in a constant-temperature oscillator at 35-45 ℃ for continuous enzymolysis for 3-4h, cooling to room temperature, adding a buffer solution to adjust the pH value of the solution to 6.5-7.5, and obtaining the fish protein mixed peptide solution.
7. The fertilizer synergist according to claim 6, wherein the fishy smell removing solution comprises the following components in parts by weight: 60-90 parts of ethanol, 20-30 parts of acetic acid, 8-15 parts of baking soda, 6-8 parts of citric acid and 5-11 parts of ginger juice.
8. The fertilizer synergist according to claim 7, wherein the compound protease comprises a composition of trypsin, chymotrypsin, neutral protease, alkaline protease, papain and flavourzyme, and the mass ratio of the trypsin, the chymotrypsin, the neutral protease, the alkaline protease, the papain and the flavourzyme is 2-3:1-2.5:1.1-2.1:0.8-1.5:0.9-1.4: 1.1-1.5.
9. The fertilizer synergist of claim 7, wherein said buffer is a citrate buffer.
10. A method of preparing a fertilizer synergist comprising a fish protein oligopeptide according to claim 8, comprising the steps of:
the method comprises the following steps: taking the fish protein mixed peptide solution, the soil loosening agent, the medium trace elements and the crop growth regulator according to the formula ratio, adding water, and uniformly stirring to obtain a mixed solution.
Step two: and (3) performing filter pressing on the solution obtained in the step one through a filter press, adding 2-5 parts of boric acid into the obtained filtrate, adjusting the pH of the solution to 7.5-8.5 by using a hydrochloric acid solution containing 3-5% of acetic acid, performing evaporation concentration until the concentration of a fixed object is more than or equal to 8%, and subpackaging.
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Cited By (3)
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| CN113831201A (en) * | 2021-08-03 | 2021-12-24 | 浙大宁波理工学院 | Method for producing organic matter-containing foliar fertilizer by using fish meal squeezed liquid |
| CN117209312A (en) * | 2023-08-29 | 2023-12-12 | 广州优卡思农业技术有限公司 | Fertilizer synergist containing fish protein oligopeptide and preparation method thereof |
| CN118026783A (en) * | 2024-03-18 | 2024-05-14 | 山东爱福地生物股份有限公司 | Fish protein peptide metal chelating composition and preparation method and application thereof |
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