CN116949140A - 分子编程工具 - Google Patents

分子编程工具 Download PDF

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Publication number
CN116949140A
CN116949140A CN202310113607.XA CN202310113607A CN116949140A CN 116949140 A CN116949140 A CN 116949140A CN 202310113607 A CN202310113607 A CN 202310113607A CN 116949140 A CN116949140 A CN 116949140A
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domain
molecule
primer
hairpin
molecular
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English (en)
Chinese (zh)
Inventor
J·Y·岸
T·E·绍斯
尹鹏
宣锋
N·哥帕克利什南
S·宇
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Harvard University
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Harvard University
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/127—DNAzymes
    • C—CHEMISTRY; METALLURGY
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    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)
CN202310113607.XA 2016-02-17 2017-02-16 分子编程工具 Pending CN116949140A (zh)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US201662296310P 2016-02-17 2016-02-17
US62/296,310 2016-02-17
US201662299206P 2016-02-24 2016-02-24
US62/299,206 2016-02-24
US201662429149P 2016-12-02 2016-12-02
US62/429,149 2016-12-02
US201662432017P 2016-12-09 2016-12-09
US62/432,017 2016-12-09
CN201780019856.5A CN108779486B (zh) 2016-02-17 2017-02-16 分子编程工具
PCT/US2017/018086 WO2017143006A1 (en) 2016-02-17 2017-02-16 Molecular programming tools

Related Parent Applications (1)

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CN201780019856.5A Division CN108779486B (zh) 2016-02-17 2017-02-16 分子编程工具

Publications (1)

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CN116949140A true CN116949140A (zh) 2023-10-27

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CN202310113607.XA Pending CN116949140A (zh) 2016-02-17 2017-02-16 分子编程工具
CN201780019856.5A Active CN108779486B (zh) 2016-02-17 2017-02-16 分子编程工具

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US (3) US11286517B2 (https=)
EP (4) EP3417078B1 (https=)
JP (1) JP7085999B2 (https=)
CN (2) CN116949140A (https=)
WO (1) WO2017143006A1 (https=)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011319755B2 (en) 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
CN116949140A (zh) * 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
EP3448886A4 (en) 2016-04-26 2019-12-11 Ultivue, Inc. SUPER-HIGH-RESOLUTION IMMUNOFLUORESCENCE WITH BINDING-LIMITED PREVIEW
AU2017259794B2 (en) 2016-05-02 2023-04-13 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
US11208676B2 (en) 2016-05-27 2021-12-28 President And Fellows Of Harvard College Conditional primer extension for single-molecule detection
WO2018017914A1 (en) 2016-07-22 2018-01-25 President And Fellows Of Harvard College Methods and compositions for protein identification
CN109923213B (zh) 2016-09-20 2023-02-28 哈佛学院院长及董事 分子验证系统
EP3568492B1 (en) 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
CN111630179B (zh) * 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
CN112189055B (zh) 2018-03-22 2024-07-19 哈佛学院院长及董事 用于分子认证的方法和组合物
WO2019236841A1 (en) 2018-06-08 2019-12-12 Ultivue, Inc. Multiplexed catalyzed reporter deposition
WO2020069386A2 (en) * 2018-09-28 2020-04-02 California Institute Of Technology Efficient combinatorial bead barcoding
JP2022513739A (ja) 2018-12-14 2022-02-09 アルティヴュー， インク． 標的を逐次検出するための方法及び組成物
WO2020185312A2 (en) * 2019-01-25 2020-09-17 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
US20220356509A1 (en) 2019-07-05 2022-11-10 Ultivue, Inc. Improved Multiplexing Method
CN114641581A (zh) * 2019-10-28 2022-06-17 深圳华大智造科技股份有限公司 Dna接头寡核苷酸
WO2021138312A1 (en) 2019-12-30 2021-07-08 Ultivue, Inc. Methods for reducing nonspecific interactions on biological samples
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
CN113156122B (zh) * 2021-04-29 2023-07-04 重庆医科大学 一种检测外泌体pd-l1的荧光传感器及其制备与应用
US20220380838A1 (en) 2021-06-01 2022-12-01 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
WO2023283442A1 (en) 2021-07-09 2023-01-12 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023108139A2 (en) 2021-12-10 2023-06-15 10X Genomics, Inc. Multi-resolution in situ decoding
WO2023129898A2 (en) 2021-12-27 2023-07-06 10X Genomics, Inc. Methods and compositions for rolling circle amplification
CN114438079B (zh) * 2021-12-31 2023-08-08 上海交通大学医学院附属仁济医院 一种仿病毒dna多面体框架结构及其制备方法与应用
EP4733410A2 (en) 2022-01-21 2026-04-29 10X Genomics, Inc. Multiple readout signals for analyzing a sample
EP4490737A1 (en) 2022-03-08 2025-01-15 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
WO2023183881A2 (en) 2022-03-24 2023-09-28 Digital Biology Inc. Tissue spatial omics
EP4500481A1 (en) 2022-03-29 2025-02-05 10X Genomics, Inc. Spectral unmixing combined with decoding for super-multiplexed in situ analysis
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
CN119234045A (zh) 2022-04-06 2024-12-31 10X基因组学有限公司 用于多重细胞分析的方法
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
CA3261351A1 (en) 2022-07-12 2024-01-18 Abrus Bio, Inc. DETERMINATION OF PROTEIN INFORMATION BY RECODED AMINO ACID POLYMERS INTO DNA POLYMERS
CN115948605A (zh) * 2022-07-13 2023-04-11 宁波华岳生物科技有限公司 一种基于dna链置换反应与per扩增的病毒检测试剂盒
EP4558647A2 (en) 2022-07-19 2025-05-28 Digital Biology Inc. Barcode diffusion-based spatial omics
WO2024040236A2 (en) 2022-08-19 2024-02-22 Abrus Bio, Inc. Determination of protein information by recoding amino acid polymers into dna polymers
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
US12372771B2 (en) 2022-11-18 2025-07-29 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
US12581050B2 (en) 2022-11-23 2026-03-17 10X Genomics, Inc. Optical assemblies for machine vision calibration
CN116334185A (zh) * 2023-04-10 2023-06-27 江苏甬泽生物科技有限公司 一种基于PER-Cas12a的核酸检测试剂盒
EP4720336A1 (en) * 2023-06-01 2026-04-08 Sherlock Biosciences, Inc. Condensed loop-mediated isothermal amplification
WO2025014758A1 (en) 2023-07-07 2025-01-16 10X Genomics, Inc. Systems and methods for tissue bounds detection
WO2025038914A1 (en) 2023-08-16 2025-02-20 10X Genomics, Inc. Systems and methods for image segmentation
WO2025076421A1 (en) 2023-10-06 2025-04-10 10X Genomics, Inc. Feature pyramiding for in situ data visualizations
US20250188524A1 (en) 2023-12-07 2025-06-12 10X Genomics, Inc. Graphical user interface and method of estimating an instrument run completion time
WO2025160489A1 (en) 2024-01-25 2025-07-31 10X Genomics, Inc. Systems and methods for image segmentation using multiple stain indicators
WO2025189080A1 (en) 2024-03-08 2025-09-12 10X Genomics, Inc. Multi-focus image fusion with background removal
US20250288988A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2026009634A1 (ja) * 2024-07-03 2026-01-08 パナソニックＩｐマネジメント株式会社 情報処理方法、情報処理システム、および情報処理プログラム
WO2026076066A1 (en) 2024-10-01 2026-04-09 10X Genomics, Inc. Systems and methods for illumination of a sample

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5030566A (en) 1989-01-04 1991-07-09 Board Of Regents, The University Of Texas System Concatemeric DNA length standards
US5543507A (en) 1992-03-05 1996-08-06 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
ES2546450T3 (es) * 2000-04-07 2015-09-23 Eiken Kagaku Kabushiki Kaisha Método para amplificar y detectar un ácido nucleico usando ácido nucleico bicatenario como molde
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
AU2002367750A1 (en) 2001-08-14 2003-11-03 Discovery, Inc. Isothermal amplification in nucleic acid analysis
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
ATE352594T1 (de) 2001-11-02 2007-02-15 November Ag Molekulare Medizin Markierungslösung zur fälschungssicheren kennzeichnung eines wertgegenstands, aus der markierungslösung hergestellte markierung sowie verfahren zur markierung eines wertgegenstands
WO2004046321A2 (en) 2002-11-15 2004-06-03 Trustees Of Boston University Cis/trans riboregulators
US20040166520A1 (en) 2003-01-03 2004-08-26 Connolly D. Michael Identifying items with nucleic acid taggants
US20070048761A1 (en) 2005-05-20 2007-03-01 Applied Dna Sciences, Inc. System and method for authenticating multiple components associated with a particular product
US20050112610A1 (en) 2003-04-16 2005-05-26 Applied Dna Sciences, Inc. System and method for marking textiles with nucleic acids
US8415164B2 (en) 2003-04-16 2013-04-09 Apdn (B.V.I.) Inc. System and method for secure document printing and detection
US8426216B2 (en) 2003-04-16 2013-04-23 APDN (B.V.I.), Inc. Methods for authenticating articles with optical reporters
AU2004232976B2 (en) 2003-04-18 2011-02-10 Becton, Dickinson And Company Immuno-amplification
NZ544959A (en) 2003-07-07 2009-02-28 One Cell Systems Inc Hairpin-labeled probes and methods of use
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
CN101048505A (zh) 2004-10-25 2007-10-03 德福根有限公司 用于递送双链rna至有害生物的包含至少一个适配子的多结构域rna分子
AU2006204087B2 (en) 2005-01-03 2010-09-16 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
US20060286569A1 (en) 2005-03-10 2006-12-21 Bar-Or Yuval A Method, apparatus, and system for authentication using labels containing nucleotide sequences
EP1896617B1 (en) 2005-05-31 2013-01-02 Life Technologies Corporation Multiplex amplification of short nucleic acids
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
US8458475B2 (en) 2005-06-20 2013-06-04 Authentiform Technologies, L.L.C. Systems and methods for product authentication
US20070026430A1 (en) 2005-06-30 2007-02-01 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension
EP1963531B1 (en) 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
EP2522751B1 (en) 2006-06-01 2018-11-21 Third Wave Technologies, Inc. Detection of nucleic acids by an improved INVADER assay
DE102006031015A1 (de) 2006-07-03 2008-01-10 Identif Gmbh Verfahren und Vorrichtung zur Authentifizierung von mit einer Markierung versehenen Gegenständen
US20100216978A1 (en) 2007-04-17 2010-08-26 Dsna-Farber Cancer Institute Inc. Wireframe nanostructures
US20080287668A1 (en) 2007-05-14 2008-11-20 Tihamer Thomas Toth-Fejel Nanostructures and methods of making
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US9340416B2 (en) 2008-08-13 2016-05-17 California Institute Of Technology Polynucleotides and related nanoassemblies, structures, arrangements, methods and systems
US20110294116A1 (en) 2008-12-11 2011-12-01 The Regents of the Unicersity of California Methods and systems for direct sequencing of single dna molecules
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
WO2011066330A2 (en) 2009-11-24 2011-06-03 Life Technologies Corporation Selective amplification of polynucleotide sequences
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
EP2524056A4 (en) 2010-01-15 2013-08-14 Univ British Columbia MULTIPLEX REINFORCEMENT FOR DETECTING NUCLEIC ACID VARIATIONS
EP2576839B1 (en) 2010-06-07 2017-05-10 Firefly Bioworks, Inc. Nucleic acid detection and quantification by post-hybridization labeling and universal encoding
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
AU2011319755B2 (en) * 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
EP2633081B1 (en) 2010-10-29 2017-01-11 Olink Bioscience AB Proximity ligation technology for western blot applications
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
US20130123121A1 (en) 2010-11-22 2013-05-16 The University Of Chicago Methods and/or Use of Oligonucleotide-Bead Conjugates for Assays and Detections
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
KR101350919B1 (ko) 2011-03-14 2014-01-14 (주)바이오니아 핵산을 포함하는 물체의 식별 방법
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
WO2013012434A1 (en) 2011-07-15 2013-01-24 University Of Miami Bioluminescent stem-loop probes, compositions containing the same and methods utilizing the same
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
WO2013138300A1 (en) 2012-03-13 2013-09-19 Authentiform Technologies, Llc Nucleic acid-based authentication codes
CA2867916C (en) 2012-03-22 2020-05-26 Lgc Genomics Limited Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
SG11201501820TA (en) 2012-09-11 2015-04-29 Theranos Inc Information management systems and methods using a biological signature
US20140272097A1 (en) 2013-03-12 2014-09-18 Applied Dna Sciences, Inc. Dna marking of previously undistinguished items for traceability
EP3216870A1 (en) 2012-11-06 2017-09-13 President and Fellows of Harvard College Riboregulator compositions and methods of use
US9243283B2 (en) 2012-11-19 2016-01-26 Src, Inc. System and method for authentication and tamper detection using nucleic acid taggants
CN103014168A (zh) 2012-12-28 2013-04-03 北京大学 基于DNA hairpin和RCA的核酸检测方法
CN106062211A (zh) * 2013-12-15 2016-10-26 哈佛学院院长等 与光学超分辨率图案化相关的方法和组合物
WO2015095633A1 (en) 2013-12-20 2015-06-25 Board Of Regents, The University Of Texas System Methods and compositions related to nucleic acid circuits and signal transducers
JP6739339B2 (ja) 2014-01-15 2020-08-12 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ カバーされた配列変換ｄｎａおよび検出方法
WO2015178978A2 (en) * 2014-02-14 2015-11-26 The Board Of Regents Of The University Of Texas System Strand exchange hairpin primers that give high allelic discrimination
CN104164488B (zh) 2014-07-09 2016-11-02 青岛艾菲生物技术有限公司 一种单引物引发的核酸恒温扩增方法
WO2016011089A1 (en) 2014-07-14 2016-01-21 President And Fellows Of Harvard College Compositions comprising riboregulators and methods of use thereof
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
WO2016140726A2 (en) 2014-12-16 2016-09-09 President And Fellows Of Harvard College Triggered assembly of metafluorophores
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
CN116949140A (zh) * 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
CN109923213B (zh) 2016-09-20 2023-02-28 哈佛学院院长及董事 分子验证系统
EP3568492B1 (en) 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
CN112189055B (zh) 2018-03-22 2024-07-19 哈佛学院院长及董事 用于分子认证的方法和组合物

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