CN116919856A - Preparation process and application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil - Google Patents
Preparation process and application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil Download PDFInfo
- Publication number
- CN116919856A CN116919856A CN202310606563.4A CN202310606563A CN116919856A CN 116919856 A CN116919856 A CN 116919856A CN 202310606563 A CN202310606563 A CN 202310606563A CN 116919856 A CN116919856 A CN 116919856A
- Authority
- CN
- China
- Prior art keywords
- oil
- peony seed
- camellia
- skin care
- olive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003921 oil Substances 0.000 title claims abstract description 118
- 235000019198 oils Nutrition 0.000 title claims abstract description 111
- 235000006484 Paeonia officinalis Nutrition 0.000 title claims abstract description 68
- 235000015112 vegetable and seed oil Nutrition 0.000 title claims abstract description 54
- 235000008390 olive oil Nutrition 0.000 title claims abstract description 50
- 239000004006 olive oil Substances 0.000 title claims abstract description 50
- 239000010495 camellia oil Substances 0.000 title claims abstract description 46
- 230000003020 moisturizing effect Effects 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 244000170916 Paeonia officinalis Species 0.000 title 1
- 241000736199 Paeonia Species 0.000 claims abstract description 67
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims description 42
- 239000000839 emulsion Substances 0.000 claims description 33
- 244000242564 Osmanthus fragrans Species 0.000 claims description 29
- 235000019083 Osmanthus fragrans Nutrition 0.000 claims description 29
- 238000001035 drying Methods 0.000 claims description 23
- 238000002156 mixing Methods 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 20
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 17
- 239000003963 antioxidant agent Substances 0.000 claims description 16
- 235000006708 antioxidants Nutrition 0.000 claims description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 239000007853 buffer solution Substances 0.000 claims description 15
- 239000010779 crude oil Substances 0.000 claims description 15
- 239000003995 emulsifying agent Substances 0.000 claims description 15
- 238000002386 leaching Methods 0.000 claims description 15
- 230000007935 neutral effect Effects 0.000 claims description 15
- 239000008055 phosphate buffer solution Substances 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 15
- 239000002904 solvent Substances 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 240000001548 Camellia japonica Species 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 240000007817 Olea europaea Species 0.000 claims description 10
- 235000018597 common camellia Nutrition 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 10
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 235000013399 edible fruits Nutrition 0.000 claims description 10
- 229940088598 enzyme Drugs 0.000 claims description 10
- 238000001704 evaporation Methods 0.000 claims description 10
- 239000003208 petroleum Substances 0.000 claims description 10
- 238000002390 rotary evaporation Methods 0.000 claims description 10
- 238000000967 suction filtration Methods 0.000 claims description 10
- 238000000194 supercritical-fluid extraction Methods 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 8
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical group CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 claims description 6
- 239000011668 ascorbic acid Substances 0.000 claims description 6
- 235000010323 ascorbic acid Nutrition 0.000 claims description 6
- 229960005070 ascorbic acid Drugs 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 5
- 241000628997 Flos Species 0.000 claims description 5
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 239000001273 butane Substances 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 claims description 5
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims description 5
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 238000010008 shearing Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 239000000600 sorbitol Substances 0.000 claims description 5
- 238000003815 supercritical carbon dioxide extraction Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 235000019082 Osmanthus Nutrition 0.000 claims description 2
- 241000333181 Osmanthus Species 0.000 claims description 2
- 239000003974 emollient agent Substances 0.000 claims 5
- 238000004519 manufacturing process Methods 0.000 claims 3
- 239000008158 vegetable oil Substances 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 4
- 150000004670 unsaturated fatty acids Chemical class 0.000 abstract description 4
- 235000021122 unsaturated fatty acids Nutrition 0.000 abstract description 4
- 206010061218 Inflammation Diseases 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 230000004054 inflammatory process Effects 0.000 abstract description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract description 2
- 235000013824 polyphenols Nutrition 0.000 abstract description 2
- 230000002000 scavenging effect Effects 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 73
- 230000000052 comparative effect Effects 0.000 description 6
- 239000004250 tert-Butylhydroquinone Substances 0.000 description 5
- 235000019281 tert-butylhydroquinone Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009993 protective function Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention relates to the technical field of vegetable oil skin care, and provides a preparation process and application of oil skin care and moisturizing oil by combining peony seed oil, olive oil and camellia oil, wherein the peony seed oil contains more unsaturated fatty acid and can play roles of scavenging free radicals, resisting inflammation and infection and enhancing skin immunity as skin care products; secondly, the olive oil is rich in unsaturated fatty acid and polyphenol substances, has excellent antioxidant effect, and the antioxidant effect of the skin-moistening oil can be enhanced to a great extent by adding the olive oil into the skin-moistening oil, so that the skin dullness speed is slowed down.
Description
Technical Field
The invention relates to the technical field of vegetable oil skin care, in particular to a preparation process and application of oil skin care and moisturizing oil combined by peony seed oil, olive oil and camellia oil.
Background
The vegetable oil is a natural high molecular compound formed by combining fatty acid and glycerin, the effect of the oil on skin is mainly represented by a protective function, the oil can form a protective film on the surface of the skin, the invasion of external harmful substances is prevented to a certain extent, and the injury of various natural factors is resisted. The oil and fat can be used for water and dry skin and hardened horny layer, so that the horny layer becomes soft, and the skin maintains an elastic and smooth health state. The skin is required to be maintained, hydrophilic substances or natural oil are selected to maintain the moisture content of the stratum corneum, and the natural oil has quite good skin-friendly property and is suitable for human skin, so most cosmetics select natural substances when selecting additives, and the natural oil is favored by cosmetics, besides the skin-friendly property, the permeability of the natural oil and the natural oil contains fatty acid required by human body, so that the natural oil has a plurality of benefits for the health of people.
At present, a plurality of skin care products based on vegetable oil appear on the market, but the skin care products still do not reach ideal optimal state in the antioxidant effect of skin, so how to design a new skin care component based on vegetable oil, which can achieve better antioxidant effect, becomes a technical problem to be solved urgently by those skilled in the art.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects existing in the prior art, the invention provides a preparation process and application of oil-based skin care and moisturizing oil combined by peony seed oil, olive oil and camellia oil, which can effectively solve the problem that the skin care product based on vegetable oil in the prior art is poor in antioxidation effect.
Technical proposal
In order to achieve the above purpose, the invention is realized by the following technical scheme:
a preparation process of oil-in-oil skin care and moisturizing oil combined by peony seed oil, olive oil and camellia oil, which comprises the following steps:
s1, selecting fresh, full and pest-free peony seeds, removing shells, drying in a drying oven at 45 ℃ until the water content is 3.5%, then pouring into a pulverizer for pulverizing, sieving with a 40-50 mesh sieve, pouring the sieved powder into a subcritical extractor, and performing extraction according to the following steps of 2:3 (g/mL) and butane as an extracting agent, wherein the extraction times are 5 times, each time of extraction is 40min, the extraction temperature is 50 ℃, and the obtained product is recorded as peony seed crude oil after rotary evaporation of the solvent;
s2, dissolving the peony seed crude oil in the S1 in normal hexane, balancing a solid phase extraction column by using methanol and normal hexane with the same volume, performing vacuum suction filtration, evaporating the solvent by using a rotary evaporator after suction filtration, and obtaining the peony seed oil;
s3, crushing fresh olive fruit pulp, adding phosphate buffer solution with the weight of 5 times of the crushed fresh olive fruit pulp, mixing and stirring, adding acid cellulase according to the enzyme adding amount of 12U/mg, stirring uniformly again, performing enzymolysis for 4 hours at 55 ℃, performing enzyme deactivation at 95 ℃ for 10 minutes after enzymolysis, leaching filter residues for 3 times by using petroleum ether after leaching, performing leaching each time, combining 4 times of filter liquor, repeatedly extracting for 3 times, performing rotary evaporation to remove solvent after extracting, and obtaining olive oil;
s4, selecting fresh camellia, drying in an oven at 40-45 ℃ for 2 hours, crushing the dried camellia to pass through a 50-mesh sieve, pouring the crushed and sieved powder into an extraction kettle, and then placing the extraction kettle into a supercritical carbon dioxide extraction instrument for supercritical extraction, wherein the obtained camellia oil is marked;
s5, according to the following steps of 1:1:2, mixing the peony seed oil, the olive oil and the camellia oil according to the volume ratio, and obtaining the oil-based skin care and moisturizing oil which is prepared by combining the peony seed oil, the olive oil and the camellia oil.
Further, the volume ratio of the peony seed crude oil to the n-hexane in the S2 is 5:12.
further, the preparation method of the phosphate buffer solution in the step S3 comprises the following steps: 92g of potassium dihydrogen phosphate is weighed, dissolved by deionized water and then fixed to 1000mL, and the phosphate buffer solution is obtained.
Further, the stirring speed in the step S3 is 300-400r/min, and ultrasonic assistance is performed in the enzymolysis process in the step S3, wherein the ultrasonic power of the ultrasonic assistance is 70W, and the ultrasonic time is 30min.
Furthermore, the supercritical extraction in the step S4 is carried out under the conditions that the extraction pressure is 35Mpa, the extraction temperature is 40 ℃ and the extraction time is 2h.
An application of oil-based skin care and moisturizing oil combined by peony seed oil, olive oil and camellia oil is characterized in that the oil-based skin care and moisturizing oil is mixed with an osmanthus oil extract, an emulsifying agent and a compound antioxidant to be processed into skin care emulsion for skin care;
the specific steps for processing the skin care emulsion are as follows:
step1, weighing 60g of disodium hydrogen phosphate, dissolving with deionized water, and then fixing the volume to 1000mL to obtain a neutral buffer solution, adding oil-based skin care and skin moisturizing oil which is prepared by combining peony seed oil, olive oil and camellia oil in S5 with the volume of 5% of the neutral buffer solution, adding osmanthus fragrans oil extract with the volume of 1-2% of the neutral buffer solution and 0.3-0.5% of an emulsifying agent, shearing and mixing under the condition of 19000r/min, homogenizing for 3 times under the condition of 600bar under high pressure, and obtaining the O/W emulsion component;
step2, adding 0.1% of composite antioxidant into the O/W emulsion component in Step1, homogenizing under high pressure at 600bar again, and obtaining the skin care emulsion.
Further, the preparation method of the osmanthus fragrans oil extract comprises the following steps: selecting fresh osmanthus fragrans, drying in an oven at 40-42 ℃ for 2 hours, crushing the dried osmanthus fragrans into 40-mesh sieve, pouring crushed osmanthus fragrans powder into filter paper, folding the filter paper, and putting the filter paper into a Soxhlet extractor according to the following formula 1:20, adding petroleum ether, extracting in constant temperature water bath at 85deg.C for 4 hr, and rotary evaporating to obtain flos Osmanthi Fragrantis oil extract.
Still further, the compound antioxidant is tert-butylhydroquinone and ascorbic acid according to 1:4 weight ratio.
Still further, the emulsifier in Step1 is polyethylene oxide sorbitol monolaurate.
Further, the time of the shear mixing in Step1 is 3-5min.
Advantageous effects
The invention provides a preparation process and application of oil-based skin care and moisturizing oil combined by peony seed oil, olive oil and camellia oil, and compared with the prior art, the preparation process has the following beneficial effects:
1. the invention takes the peony seed oil, the olive oil and the camellia oil as raw materials to prepare the skin-moistening oil, the peony seed oil contains more unsaturated fatty acid, and can play roles in scavenging free radicals, resisting inflammation and infection and enhancing skin immunity as skin care products; secondly, the olive oil is rich in unsaturated fatty acid and polyphenol substances, has excellent antioxidant effect, and the antioxidant effect of the skin-moistening oil can be enhanced to a great extent by adding the olive oil into the skin-moistening oil, so that the skin dullness speed is slowed down.
2. In the application of the skin-care oil, the antioxidant stability of the skin-care oil can be obviously improved through the synergistic effect between the tertiary butyl hydroquinone and the ascorbic acid, and the osmanthus fragrans oil is added in the preparation of the skin-care emulsion, so that the anti-inflammatory and sterilizing effects of the skin-care emulsion can be enhanced, and the skin-care effect of the oil is enhanced to a certain extent.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. It will be apparent that the described embodiments are some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention is further described below with reference to examples.
Example 1
The preparation process of the oil-based skin care and moisturizing oil with the combination of peony seed oil, olive oil and camellia oil comprises the following steps of:
s1, selecting fresh, full and pest-free peony seeds, removing shells, drying in a drying oven at 45 ℃ until the water content is 3.5%, then pouring into a pulverizer to pulverize, sieving with a 40-mesh sieve, pouring the sieved powder into a subcritical extractor, and performing extraction according to the following steps of 2:3 (g/mL) and butane as an extracting agent, wherein the extraction times are 5 times, each time of extraction is 40min, the extraction temperature is 50 ℃, and the obtained product is recorded as peony seed crude oil after rotary evaporation of the solvent;
s2, dissolving the peony seed crude oil in the S1 in normal hexane, balancing a solid phase extraction column by using methanol and normal hexane with the same volume, performing vacuum suction filtration, evaporating the solvent by using a rotary evaporator after suction filtration, and obtaining the peony seed oil;
s3, crushing fresh olive fruit pulp, adding phosphate buffer solution with the weight of 5 times of the crushed fresh olive fruit pulp, mixing and stirring, adding acid cellulase according to the enzyme adding amount of 12U/mg, stirring uniformly again, performing enzymolysis for 4 hours at 55 ℃, performing enzyme deactivation at 95 ℃ for 10 minutes after enzymolysis, leaching filter residues for 3 times by using petroleum ether after leaching, performing leaching each time, combining 4 times of filter liquor, repeatedly extracting for 3 times, performing rotary evaporation to remove solvent after extracting, and obtaining olive oil;
s4, selecting fresh camellia, drying in a baking oven at 40 ℃ for 2 hours, crushing the dried camellia to pass through a 50-mesh sieve, pouring the crushed and sieved powder into an extraction kettle, and then placing the extraction kettle into a supercritical carbon dioxide extraction instrument for supercritical extraction, wherein the obtained camellia oil is recorded;
s5, according to the following steps of 1:1:2, mixing the peony seed oil, the olive oil and the camellia oil according to the volume ratio, and obtaining the oil-based skin care and moisturizing oil which is prepared by combining the peony seed oil, the olive oil and the camellia oil.
In the S2, the volume ratio of the peony seed crude oil to the normal hexane is 5:12.
the preparation method of the phosphate buffer solution in the S3 comprises the following steps: 92g of potassium dihydrogen phosphate is weighed, dissolved by deionized water and then fixed to 1000mL, and the phosphate buffer solution is obtained.
The stirring speed in the step S3 is 300r/min, ultrasonic assistance is carried out in the enzymolysis process in the step S3, the ultrasonic power of ultrasonic assistance is 70W, and the ultrasonic time is 30min.
The supercritical extraction pressure in S4 is 35Mpa, the extraction temperature is 40 ℃, and the extraction time is 2h.
An application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil is disclosed, wherein the oil-based skin care oil is prepared by mixing and processing the oil-based skin care oil with an osmanthus fragrans oil extract, an emulsifying agent and a compound antioxidant to obtain skin care emulsion for skin care;
the skin care emulsion is prepared by the following specific steps:
step1, weighing 60g of disodium hydrogen phosphate, dissolving with deionized water, and then fixing the volume to 1000mL to obtain a neutral buffer solution, adding oil-based skin care and skin moisturizing oil which is prepared by combining peony seed oil, olive oil and camellia oil in S5 with the volume of 5% of the neutral buffer solution, adding osmanthus fragrans oil extract with the volume of 1% of the neutral buffer solution and 0.3% of an emulsifying agent, shearing and mixing under the condition of 19000r/min, homogenizing for 3 times under the condition of 600bar under high pressure, and obtaining the O/W emulsion component;
step2, adding 0.1% of composite antioxidant into the O/W emulsion component in Step1, homogenizing under high pressure at 600bar again, and obtaining the skin care emulsion.
The preparation method of the osmanthus fragrans oil extract comprises the following steps: selecting fresh osmanthus fragrans, drying in a baking oven at 40 ℃ for 2 hours, crushing the dried osmanthus fragrans into 40-mesh sieve, pouring crushed osmanthus fragrans powder into filter paper, folding the filter paper, and putting the filter paper into a Soxhlet extractor according to the following formula 1:20, adding petroleum ether, extracting in constant temperature water bath at 85deg.C for 4 hr, and rotary evaporating to obtain flos Osmanthi Fragrantis oil extract.
The compound antioxidant is tert-butyl hydroquinone and ascorbic acid according to the following ratio of 1:4 weight ratio.
The emulsifier in Step1 is polyethylene oxide sorbitol monolaurate.
The time for shear mixing in Step1 was 3min.
Example 2
The preparation process of the oil-based skin care and moisturizing oil with the combination of peony seed oil, olive oil and camellia oil comprises the following steps of:
s1, selecting fresh, full and pest-free peony seeds, removing shells, drying in a drying oven at 45 ℃ until the water content is 3.5%, then pouring into a pulverizer for pulverizing, sieving with a 50-mesh sieve, pouring the sieved powder into a subcritical extractor, and performing extraction according to the following steps of 2:3 (g/mL) and butane as an extracting agent, wherein the extraction times are 5 times, each time of extraction is 40min, the extraction temperature is 50 ℃, and the obtained product is recorded as peony seed crude oil after rotary evaporation of the solvent;
s2, dissolving the peony seed crude oil in the S1 in normal hexane, balancing a solid phase extraction column by using methanol and normal hexane with the same volume, performing vacuum suction filtration, evaporating the solvent by using a rotary evaporator after suction filtration, and obtaining the peony seed oil;
s3, crushing fresh olive fruit pulp, adding phosphate buffer solution with the weight of 5 times of the crushed fresh olive fruit pulp, mixing and stirring, adding acid cellulase according to the enzyme adding amount of 12U/mg, stirring uniformly again, performing enzymolysis for 4 hours at 55 ℃, performing enzyme deactivation at 95 ℃ for 10 minutes after enzymolysis, leaching filter residues for 3 times by using petroleum ether after leaching, performing leaching each time, combining 4 times of filter liquor, repeatedly extracting for 3 times, performing rotary evaporation to remove solvent after extracting, and obtaining olive oil;
s4, selecting fresh camellia, drying in a drying oven at 45 ℃ for 2 hours, crushing the dried camellia to pass through a 50-mesh sieve, pouring the crushed and sieved powder into an extraction kettle, and then placing the extraction kettle into a supercritical carbon dioxide extraction instrument for supercritical extraction, wherein the obtained camellia oil is recorded;
s5, according to the following steps of 1:1:2, mixing the peony seed oil, the olive oil and the camellia oil according to the volume ratio, and obtaining the oil-based skin care and moisturizing oil which is prepared by combining the peony seed oil, the olive oil and the camellia oil.
In the S2, the volume ratio of the peony seed crude oil to the normal hexane is 5:12.
the preparation method of the phosphate buffer solution in the S3 comprises the following steps: 92g of potassium dihydrogen phosphate is weighed, dissolved by deionized water and then fixed to 1000mL, and the phosphate buffer solution is obtained.
The stirring speed in the step S3 is 400r/min, ultrasonic assistance is carried out in the enzymolysis process in the step S3, the ultrasonic power of ultrasonic assistance is 70W, and the ultrasonic time is 30min.
The supercritical extraction pressure in S4 is 35Mpa, the extraction temperature is 40 ℃, and the extraction time is 2h.
An application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil is disclosed, wherein the oil-based skin care oil is prepared by mixing and processing the oil-based skin care oil with an osmanthus fragrans oil extract, an emulsifying agent and a compound antioxidant to obtain skin care emulsion for skin care;
the skin care emulsion is prepared by the following specific steps:
step1, weighing 60g of disodium hydrogen phosphate, dissolving with deionized water, and then fixing the volume to 1000mL to obtain a neutral buffer solution, adding oil-based skin care and skin moisturizing oil which is prepared by combining peony seed oil, olive oil and camellia oil in S5 with the volume of 5% of the neutral buffer solution, adding an osmanthus fragrans oil extract with the volume of 2% of the neutral buffer solution and 0.5% of an emulsifying agent, shearing and mixing under the condition of 19000r/min, homogenizing for 3 times under the condition of 600bar under high pressure, and obtaining the O/W emulsion component;
step2, adding 0.1% of composite antioxidant into the O/W emulsion component in Step1, homogenizing under high pressure at 600bar again, and obtaining the skin care emulsion.
The preparation method of the osmanthus fragrans oil extract comprises the following steps: selecting fresh osmanthus fragrans, drying in a drying oven at 42 ℃ for 2 hours, crushing the dried osmanthus fragrans into 40-mesh sieve, pouring crushed osmanthus fragrans powder into filter paper, folding the filter paper, and putting the filter paper into a Soxhlet extractor according to the following formula 1:20, adding petroleum ether, extracting in constant temperature water bath at 85deg.C for 4 hr, and rotary evaporating to obtain flos Osmanthi Fragrantis oil extract.
The compound antioxidant is tert-butyl hydroquinone and ascorbic acid according to the following ratio of 1:4 weight ratio.
The emulsifier in Step1 is polyethylene oxide sorbitol monolaurate.
The time for shear mixing in Step1 was 5min.
Example 3
The preparation process of the oil-based skin care and moisturizing oil with the combination of peony seed oil, olive oil and camellia oil comprises the following steps of:
s1, selecting fresh, full and pest-free peony seeds, removing shells, drying in a drying oven at 45 ℃ until the water content is 3.5%, then pouring into a pulverizer for pulverizing, sieving with a 45-mesh sieve, pouring the sieved powder into a subcritical extractor, and performing extraction according to the following steps of 2:3 (g/mL) and butane as an extracting agent, wherein the extraction times are 5 times, each time of extraction is 40min, the extraction temperature is 50 ℃, and the obtained product is recorded as peony seed crude oil after rotary evaporation of the solvent;
s2, dissolving the peony seed crude oil in the S1 in normal hexane, balancing a solid phase extraction column by using methanol and normal hexane with the same volume, performing vacuum suction filtration, evaporating the solvent by using a rotary evaporator after suction filtration, and obtaining the peony seed oil;
s3, crushing fresh olive fruit pulp, adding phosphate buffer solution with the weight of 5 times of the crushed fresh olive fruit pulp, mixing and stirring, adding acid cellulase according to the enzyme adding amount of 12U/mg, stirring uniformly again, performing enzymolysis for 4 hours at 55 ℃, performing enzyme deactivation at 95 ℃ for 10 minutes after enzymolysis, leaching filter residues for 3 times by using petroleum ether after leaching, performing leaching each time, combining 4 times of filter liquor, repeatedly extracting for 3 times, performing rotary evaporation to remove solvent after extracting, and obtaining olive oil;
s4, selecting fresh camellia, drying in a drying oven at 42 ℃ for 2 hours, crushing the dried camellia to pass through a 50-mesh sieve, pouring the crushed and sieved powder into an extraction kettle, and then placing the extraction kettle into a supercritical carbon dioxide extraction instrument for supercritical extraction, wherein the obtained camellia oil is recorded;
s5, according to the following steps of 1:1:2, mixing the peony seed oil, the olive oil and the camellia oil according to the volume ratio, and obtaining the oil-based skin care and moisturizing oil which is prepared by combining the peony seed oil, the olive oil and the camellia oil.
In the S2, the volume ratio of the peony seed crude oil to the normal hexane is 5:12.
the preparation method of the phosphate buffer solution in the S3 comprises the following steps: 92g of potassium dihydrogen phosphate is weighed, dissolved by deionized water and then fixed to 1000mL, and the phosphate buffer solution is obtained.
The stirring speed in the step S3 is 300r/min, ultrasonic assistance is carried out in the enzymolysis process in the step S3, the ultrasonic power of ultrasonic assistance is 70W, and the ultrasonic time is 30min.
The supercritical extraction pressure in S4 is 35Mpa, the extraction temperature is 40 ℃, and the extraction time is 2h.
An application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil is disclosed, wherein the oil-based skin care oil is prepared by mixing and processing the oil-based skin care oil with an osmanthus fragrans oil extract, an emulsifying agent and a compound antioxidant to obtain skin care emulsion for skin care;
the skin care emulsion is prepared by the following specific steps:
step1, weighing 60g of disodium hydrogen phosphate, dissolving with deionized water, and then fixing the volume to 1000mL to obtain a neutral buffer solution, adding oil-based skin care and skin moisturizing oil which is prepared by combining peony seed oil, olive oil and camellia oil in S5 with the volume of 5% of the neutral buffer solution, adding an osmanthus fragrans oil extract with the volume of 2% of the neutral buffer solution and 0.4% of an emulsifying agent, shearing and mixing under the condition of 19000r/min, homogenizing for 3 times under the condition of 600bar under high pressure, and obtaining the O/W emulsion component;
step2, adding 0.1% of composite antioxidant into the O/W emulsion component in Step1, homogenizing under high pressure at 600bar again, and obtaining the skin care emulsion.
The preparation method of the osmanthus fragrans oil extract comprises the following steps: selecting fresh osmanthus fragrans, drying in a baking oven at 41 ℃ for 2 hours, crushing the dried osmanthus fragrans into 40-mesh sieve, pouring crushed osmanthus fragrans powder into filter paper, folding the filter paper, and putting the filter paper into a Soxhlet extractor according to the following formula 1:20, adding petroleum ether, extracting in constant temperature water bath at 85deg.C for 4 hr, and rotary evaporating to obtain flos Osmanthi Fragrantis oil extract.
The compound antioxidant is tert-butyl hydroquinone and ascorbic acid according to the following ratio of 1:4 weight ratio.
The emulsifier in Step1 is polyethylene oxide sorbitol monolaurate.
The time for shear mixing in Step1 was 4min.
Comparative example
The comparative example is a skin care emulsion which is randomly purchased on the market.
Performance testing
The skin care emulsions prepared in examples 1 to 3 were labeled as experimental example 1, experimental example 2 and experimental example 3, respectively, and the skin care emulsions in the comparative examples were labeled as comparative examples.
The skin care emulsions in experimental example 1, experimental example 2, experimental example 3 and comparative example were tested by selecting 4 volunteers with similar skin properties, and the testing method is as follows: 1mL of skin care emulsion is smeared on the face after cleansing the face in the morning and evening every day, the test period is 14 days, and the skin condition of 4 volunteers is evaluated in 14 days, wherein the evaluation grade of the skin condition is from first grade to fourth grade, and the more serious the skin condition is, the higher the grade is. The evaluation results are recorded in table 1:
TABLE 1
The data in Table 1 shows that the skin darkening conditions of volunteers in experimental examples 1-3 and comparative examples are four grades on the first day, but the skin darkening conditions of volunteers in experimental examples 1-3 are obviously improved on the 7 th day and the 14 th day, so that the oil-in-oil skin-nourishing skin-moistening oil prepared by the invention and combining peony seed oil, olive oil and camellia oil can slow down the skin darkening speed to a certain extent after being applied to prepare skin-care emulsion, and has better market popularization value.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The preparation process of the oil-based skin care and moisturizing oil combining the peony seed oil, the olive oil and the camellia oil is characterized by comprising the following steps of:
s1, selecting fresh, full and pest-free peony seeds, removing shells, drying in a drying oven at 45 ℃ until the water content is 3.5%, then pouring into a pulverizer for pulverizing, sieving with a 40-50 mesh sieve, pouring the sieved powder into a subcritical extractor, and performing extraction according to the following steps of 2:3 (g/mL) and butane as an extracting agent, wherein the extraction times are 5 times, each time of extraction is 40min, the extraction temperature is 50 ℃, and the obtained product is recorded as peony seed crude oil after rotary evaporation of the solvent;
s2, dissolving the peony seed crude oil in the S1 in normal hexane, balancing a solid phase extraction column by using methanol and normal hexane with the same volume, performing vacuum suction filtration, evaporating the solvent by using a rotary evaporator after suction filtration, and obtaining the peony seed oil;
s3, crushing fresh olive fruit pulp, adding phosphate buffer solution with the weight of 5 times of the crushed fresh olive fruit pulp, mixing and stirring, adding acid cellulase according to the enzyme adding amount of 12U/mg, stirring uniformly again, performing enzymolysis for 4 hours at 55 ℃, performing enzyme deactivation at 95 ℃ for 10 minutes after enzymolysis, leaching filter residues for 3 times by using petroleum ether after leaching, performing leaching each time, combining 4 times of filter liquor, repeatedly extracting for 3 times, performing rotary evaporation to remove solvent after extracting, and obtaining olive oil;
s4, selecting fresh camellia, drying in an oven at 40-45 ℃ for 2 hours, crushing the dried camellia to pass through a 50-mesh sieve, pouring the crushed and sieved powder into an extraction kettle, and then placing the extraction kettle into a supercritical carbon dioxide extraction instrument for supercritical extraction, wherein the obtained camellia oil is marked;
s5, according to the following steps of 1:1:2, mixing the peony seed oil, the olive oil and the camellia oil according to the volume ratio, and obtaining the oil-based skin care and moisturizing oil which is prepared by combining the peony seed oil, the olive oil and the camellia oil.
2. The process for preparing the oil-in-oil skin care and moisturizing oil by combining peony seed oil, olive oil and camellia oil according to claim 1, wherein the volume ratio of the peony seed crude oil to the n-hexane in the S2 is 5:12.
3. the process for preparing the oil-in-oil skin care and moisturizing oil by combining peony seed oil, olive oil and camellia oil according to claim 1, wherein the preparation method of the phosphate buffer solution in the step S3 is as follows: 92g of potassium dihydrogen phosphate is weighed, dissolved by deionized water and then fixed to 1000mL, and the phosphate buffer solution is obtained.
4. The preparation process of the oil-in-oil skin care and moisturizing oil combining peony seed oil, olive oil and camellia oil according to claim 1, wherein the stirring speed in the step S3 is 300-400r/min, ultrasonic assistance is performed in the enzymolysis process in the step S3, the ultrasonic power of the ultrasonic assistance is 70W, and the ultrasonic time is 30min.
5. The process for preparing the oil-in-oil skin care and moisturizing oil by combining peony seed oil, olive oil and camellia oil according to claim 1, wherein the supercritical extraction in the step S4 is carried out at an extraction pressure of 35Mpa, an extraction temperature of 40 ℃ and an extraction time of 2h.
6. The use of oil-in-oil skin care emollient oil prepared by the preparation process of any one of claims 1-5 in combination with peony seed oil, olive oil and camellia oil, wherein the use is in combination with osmanthus oil extract, emulsifier and complex antioxidant to prepare skin care emulsion for skin care;
the specific steps for processing the skin care emulsion are as follows:
step1, weighing 60g of disodium hydrogen phosphate, dissolving with deionized water, and then fixing the volume to 1000mL to obtain a neutral buffer solution, adding oil-based skin care and skin moisturizing oil which is prepared by combining peony seed oil, olive oil and camellia oil in S5 with the volume of 5% of the neutral buffer solution, adding osmanthus fragrans oil extract with the volume of 1-2% of the neutral buffer solution and 0.3-0.5% of an emulsifying agent, shearing and mixing under the condition of 19000r/min, homogenizing for 3 times under the condition of 600bar under high pressure, and obtaining the O/W emulsion component;
step2, adding 0.1% of composite antioxidant into the O/W emulsion component in Step1, homogenizing under high pressure at 600bar again, and obtaining the skin care emulsion.
7. The use of an oil-in-skin emollient oil of a combination of peony seed oil, olive oil and camellia oil according to claim 6, wherein the preparation method of the osmanthus fragrans oil extract is: selecting fresh osmanthus fragrans, drying in an oven at 40-42 ℃ for 2 hours, crushing the dried osmanthus fragrans into 40-mesh sieve, pouring crushed osmanthus fragrans powder into filter paper, folding the filter paper, and putting the filter paper into a Soxhlet extractor according to the following formula 1:20, adding petroleum ether, extracting in constant temperature water bath at 85deg.C for 4 hr, and rotary evaporating to obtain flos Osmanthi Fragrantis oil extract.
8. The use of an oil-in-skin emollient oil in combination of peony seed oil, olive oil and camellia oil according to claim 6, wherein the compound antioxidant is t-butylhydroquinone and ascorbic acid according to 1:4 weight ratio.
9. The use of an oil-in-skin emollient oil in combination of peony seed oil, olive oil and camellia oil according to claim 6, wherein the emulsifier in Step1 is polyethylene oxide sorbitol monolaurate.
10. The use of an oil-in-skin emollient oil in combination of peony seed oil, olive oil and camellia oil according to claim 6, wherein the time of shear mixing in Step1 is 3-5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310606563.4A CN116919856A (en) | 2023-05-26 | 2023-05-26 | Preparation process and application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310606563.4A CN116919856A (en) | 2023-05-26 | 2023-05-26 | Preparation process and application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116919856A true CN116919856A (en) | 2023-10-24 |
Family
ID=88374501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310606563.4A Pending CN116919856A (en) | 2023-05-26 | 2023-05-26 | Preparation process and application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116919856A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104277908A (en) * | 2014-09-25 | 2015-01-14 | 齐鲁工业大学 | Preparation method of peony seed oil |
| CN105255577A (en) * | 2015-09-06 | 2016-01-20 | 中国林业科学研究院林产化学工业研究所 | Method for high-efficiency extraction of olive oil by utilization of aqueous enzymatic method |
| CN106336947A (en) * | 2016-11-21 | 2017-01-18 | 菏泽尧舜牡丹生物科技有限公司 | Production process for extracting peony seed oil |
| CN108949340A (en) * | 2018-09-14 | 2018-12-07 | 安徽大佛山养心谷旅游开发有限公司 | A kind of preparation method of peony seed oil |
| CN113876635A (en) * | 2021-10-28 | 2022-01-04 | 德化县祥山大果油茶有限公司 | Camellia skin moistening and repairing essence oil and preparation equipment thereof |
| CN114129476A (en) * | 2021-12-14 | 2022-03-04 | 上海家化联合股份有限公司 | Composition comprising vegetable fruit oil and vegetable seed oil and use thereof |
-
2023
- 2023-05-26 CN CN202310606563.4A patent/CN116919856A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104277908A (en) * | 2014-09-25 | 2015-01-14 | 齐鲁工业大学 | Preparation method of peony seed oil |
| CN105255577A (en) * | 2015-09-06 | 2016-01-20 | 中国林业科学研究院林产化学工业研究所 | Method for high-efficiency extraction of olive oil by utilization of aqueous enzymatic method |
| CN106336947A (en) * | 2016-11-21 | 2017-01-18 | 菏泽尧舜牡丹生物科技有限公司 | Production process for extracting peony seed oil |
| CN108949340A (en) * | 2018-09-14 | 2018-12-07 | 安徽大佛山养心谷旅游开发有限公司 | A kind of preparation method of peony seed oil |
| CN113876635A (en) * | 2021-10-28 | 2022-01-04 | 德化县祥山大果油茶有限公司 | Camellia skin moistening and repairing essence oil and preparation equipment thereof |
| CN114129476A (en) * | 2021-12-14 | 2022-03-04 | 上海家化联合股份有限公司 | Composition comprising vegetable fruit oil and vegetable seed oil and use thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3053368B2 (en) | Cosmetic and method for producing the same | |
| JP2005082522A (en) | Bleaching cosmetic | |
| KR101308669B1 (en) | Cosmetic composition with the nebaneba complex of hibiscus esculentus, laminaria japonica, dioscorea opposita, corchorus olitorius and nelumbo nucifera, polyglutamic acid | |
| JP2003267822A (en) | Skin care preparation | |
| JP2008184416A (en) | External preparation for skin, cell activation agent and antioxidation agent | |
| CN117017829A (en) | Antioxidant moisturizing composition and preparation method and application thereof | |
| KR100372360B1 (en) | Cosmetic compositions containing garlic and Acanthopanax extracts | |
| JP6377879B1 (en) | Topical skin preparation | |
| CN109044901B (en) | Whitening and skin-caring composition containing citrus seed extract | |
| CN116919856A (en) | Preparation process and application of oil-based skin care and moisturizing oil prepared from peony seed oil, olive oil and camellia oil | |
| KR102334348B1 (en) | Cosmetic Composition For Moisturizing Skin Comprising Mixed Extract of Petroselinum Crispum, Houttuynia Cordata, Mentha Piperita Leaf and Morinda Citrifolia as Active Ingredient | |
| KR102307139B1 (en) | Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Chondrus crispus, Origanum majorana, Petroselinum crispum and Fumaria officinalis As Active Ingredient | |
| KR101929373B1 (en) | Cosmetic composition for skin moisturizing containing extract of cardamon seed | |
| JP3591832B2 (en) | External preparation for skin | |
| JPH09143025A (en) | Cosmetic | |
| JP4748962B2 (en) | Moisturizer, cell activator, whitening agent, and antioxidant | |
| KR102625741B1 (en) | Cosmetic composition for moisturizing skin comprising mixed extracts of capparis spinosa, olea europaea, citrus sinensis and oryza sativa | |
| KR101603956B1 (en) | Cosmetic composition for skin with fermented extract of green tomato and Armillaria mellea | |
| JP7218166B2 (en) | Skin topical agent | |
| KR102308766B1 (en) | Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Ganoderma lucidum, Arnica montana, Buddleja, Thymus vulgaris, Sigesbeckia Orientalis and Rabdosia rubescens as Active Ingredient | |
| JP2004137166A (en) | Skin care preparation for external use, cell activator and antioxidant | |
| KR20190046466A (en) | Cosmetic composition for moisturizing skin comprising extract of tigernuts as active ingredient | |
| JP3429297B1 (en) | External preparation for skin | |
| KR20220026746A (en) | Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Chaenomeles sinensis fruit, Diospyros kaki leaf, Cinnamomum cassia bark, Artemisia princeps leaf, Chrysanthellum indicum and Camellia sinensis leaf as Active Ingredient | |
| Khole et al. | Formulation and Evaluation of Anti aging Cream |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |