KR20220026746A - Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Chaenomeles sinensis fruit, Diospyros kaki leaf, Cinnamomum cassia bark, Artemisia princeps leaf, Chrysanthellum indicum and Camellia sinensis leaf as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition which contains a mixed extract of Pseudocydonia sinensis, Diospyros kaki leaves, Cinnamomum cassia bark, Artemisia indica leaves, Chrysanthellum Indicum, and green tea as an active component. More specifically, the present invention provides a cosmetic composition for moisturizing the skin which has excellent skin moisturizing effect and skin barrier improvement effect.

Description

Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Chaenomeles sinensis fruit, Diospyros kaki leaf, Cinnamomum cassia bark, Artemisia princeps leaf, Chrysanthellum indicum and Camellia sinensis leaf as Active Ingredient}

The present invention relates to a cosmetic composition containing a mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea as an active ingredient, and more particularly, to a cosmetic composition for moisturizing.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of the skin that occurs naturally with aging. In general, aging is caused by oxidative damage to biological materials by free radicals. That is, highly reactive free radicals such as active oxygen such as hydrogen peroxide, hydroxy radicals, and superoxide radicals peroxidize lipids constituting cell membranes to destroy cell membranes, oxidize proteins, and attack hexane to cause oxidative damage to biomaterials destroys the cells and eventually causes the cells to die. The most significant skin characteristics due to aging include wrinkles, loss of elasticity, and dullness of the skin.

In addition, in addition to wrinkles and decreased elasticity, after the age of 40, the color of the face is not clear and often dull and dark. In particular, in the case of young women, eohyeol occurs during childbirth and blood circulation disorders occur, which leads to a decrease in metabolic ability, and causes the skin to become rough and dull. When the metabolic ability decreases and the skin becomes rough, the moisture content is also easily reduced. In general, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10 to 30% in the keratinocyte layer. It is known that a substance with hydrophilic water retention ability called Natural Moisturizing Factor (NMF) exists in the keratinocyte layer and plays an important role in moisturizing the skin. When the normal keratinocyte layer has about 10 to 30% moisture, the skin is smooth and soft, and the body's protective function is normally exhibited.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important to prevent skin aging, but other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate (Sodium lactate), urea, etc. There is a problem of impairing the emulsion stability of this strong cosmetic. Although amino acids, collagen, and elastin have moisturizing properties, there is a limit to their moisturizing ability. The ability to retain moisture in the stratum corneum can be controlled by the natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. The development of substances with high moisturizing effect is one of the important research tasks in cosmetics. In addition, substances known to have an antioxidant action include ascorbic acid, dl-alpha-tocopherol, beta-carotene (β-carotene), dibutylhydroxyltoluene (BHT), and the like. Since these substances are used as synthetic products, they can cause problems in skin safety when used for a long period of time or in large amounts, so the development of a new antioxidant cosmetic that can be safely and usefully applied to the skin is urgently required.

Therefore, research on skin aging and moisture improvement is expected to continue, and many cosmetics using natural products are being developed to solve these skin problems and reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing.

For this purpose, research to develop substances with excellent safety and excellent skin-beauty effects by searching the skin physiological activity of various natural plant components is currently being actively conducted.

Korean Patent No. 10-2032873 discloses a cosmetic composition comprising a mixed extract containing chrysanthemum extract, lotus flower extract and perilla leaf extract, and having antioxidant and moisturizing functions.

Accordingly, the present inventors have studied the possibility of application to cosmetics in various natural materials that are not known so far, and as a result, quince, persimmon leaves, cinnamon bark, mugwort leaves, chrysanthelium indicum and green tea were selected and extracts were prepared therefrom. As a result of measuring the moisturizing effect, it was found that the efficacy as a cosmetic can be expected because the efficacy is very good.

It is an object of the present invention to provide a cosmetic composition for skin protection that contains a mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea to show a moisturizing effect.

In addition, an object of the present invention is to prepare a supercritical, ultrasonic and fermented extract to maximize the efficacy of a mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea, and then go through the purification process to effect the effect To provide a cosmetic composition representing

The above object is achieved by a cosmetic composition for moisturizing the skin containing a mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea.

Preferably, the cosmetic composition has an effect of improving the skin barrier function.

Also preferably, the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea may be contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Also, preferably, the mixed extract of the quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelum indicum and green tea (a) purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloro A solvent extraction method for extraction using an extraction solvent selected from the group consisting of methane, chloroform, ethyl ether, butylene glycol, hexane and mixtures thereof, (b) supercritical extraction, and (c) ultrasonic extraction method selected from the group consisting of extraction method It is characterized in that it is extracted by

Also preferably, the mixed extract is quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea 1~3:1~3:1~3:1~3:1~3:1~3 may be included in a mixed weight ratio of

The mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea according to the present invention has excellent skin moisturizing effect and skin barrier function recovery effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include, but are not limited to, a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a cosmetic composition for moisturizing the skin containing a mixed extract of quince, persimmon leaves, broiler bark, mugwort leaves, chrysanthelium indicum and green tea.

The quince (Chaenomeles sinensis) used as an active ingredient in the cosmetic composition of the present invention is a fruit of the quince tree of the Rosaceae family of the Rosaceae. The medicinal effect of quince is generally known to be good for bronchial diseases, phlegm, and asthma, and it also helps digestion a lot. It is also very good for hangover relief. Since quince is rich in vitamin C and tannin, it is good for recovery from fatigue, and it is also good for relieving muscle pain caused by overwork as it softens the muscles. These ingredients are representative antioxidants that inhibit free radicals in the body to prevent aging of skin cells, and maintain clean skin by removing spots, freckles, blemishes, acne, and fine wrinkles. In addition, organic acids such as citric acid, malic acid, and citric acid Since it contains a large amount of ingredients, it activates the body's metabolism and is effective for skin care by discharging various wastes and toxic substances accumulated in the body to the outside of the body. The quince of the present invention uses the fruit of quince.

Persimmon tree (Diospyros kaki) is a species endemic to the temperate zone of East Asia, and is widely cultivated in northern central China, Japan, and south central Korea. Persimmon leaves are rich in vitamin C, contain a large amount of calcium and tannins, so vitamins are absorbed well by our body, and it helps to improve dry skin and wrinkles, and helps prevent skin aging. The persimmon tree of the present invention uses the leaves of the persimmon tree.

Cinnamomum cassia is an evergreen tree in the genus Camphor, and the bark of the cinnamon tree is used as cinnamon. The cinnamon bark revitalizes the skin and helps to make it lively, protects the skin and makes the skin texture soft. In addition, it is good for moisturizing the skin and helps to manage the skin tone by suppressing the production of melanin pigment. In addition, because it regulates the skin's pore contraction, it helps to manage skin elasticity and block harmful external substances to the skin. The broiler tree of the present invention uses the bark of the broiler tree.

Artemisia princeps (Artemisia princeps) is a perennial plant belonging to the Asteraceae family. Because it has strong vitality and is easily available, it is widely used for food and medicinal purposes. Mugwort contains vitamin C, which helps to prevent aging and promotes skin regeneration. It also has excellent antibacterial and disinfecting effects, so it is good for skin care such as acne. The mugwort of the present invention uses the leaves of mugwort.

Chrysanthellum indicum is the scientific name of Chrysanthemum and is a perennial plant in the Asteraceae family. As for skin efficacy, it is characterized by having antibacterial, antioxidant, anti-inflammatory, anti-wrinkle, skin elasticity, skin soothing, and skin tone improvement functions. Chrysanthelium indicum of the present invention uses an outpost of Chrysanthelium indicum.

Green tea (Camellia sinensis) refers to unfermented, dried tea leaves or water made from tea leaves. Green tea contains 5 to 8 times more vitamin C and a large amount of tocopherol than lemon. Vitamin C and tocopherol have a whitening effect that suppresses the formation of spots and freckles, that is, it suppresses the deposition of melanin, so it keeps the skin white and clean. Green tea of the present invention uses green tea leaves.

In addition, the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea can be prepared according to a conventional method known in the art. For example, extraction is performed using (a) solvent extraction, (b) supercritical extraction using reduced pressure and high temperature with carbon dioxide, or (c) ultrasonic extraction.

In the present invention, the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea is prepared using a variety of extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acids and mixtures thereof. obtainable using one or more extraction solvents, preferably obtained using ethanol, 70 % (v/v) ethanol or water, and most preferably obtained using 70 % (v/v) ethanol .

On the other hand, the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea of the present invention is obtained by using not only the above-described extraction solvent, but also an extract showing substantially the same effect even when other extraction solvents are used. It will be apparent to those skilled in the art that

In addition, the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea of the present invention includes not only the extract using the above-described extraction solvent, but also the extract that has undergone a conventional purification and fermentation process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction using ultrasound, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity The active fraction obtained through various purification and extraction methods additionally performed, such as separation by a method prepared for separation according to sex) or extracts from fermentation products using natural conditions or various microorganisms, are also included in the extract of the present invention.

In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by cooling the extract at room temperature, heating a liquid obtained by filtration, and further concentrating a solvent under reduced pressure or freeze-drying.

The extraction method by the supercritical extraction method by the reduced pressure by carbon dioxide and the high temperature refers to the supercritical fluid extraction method, and in general, the supercritical fluid is a liquid having a gas when it reaches a critical point under high temperature and high pressure conditions, and It has gaseous properties and chemically has a polarity similar to that of a non-polar solvent. Due to these properties, supercritical fluids are used for the extraction of fat-soluble substances (J. Chromatogr. A. 1998;479:200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gas properties as the pressure and temperature reach the critical point through the operation of the supercritical fluid device, and as a result, the solubility in fat-soluble solutes increases. When the supercritical carbon dioxide passes through an extraction vessel containing a certain amount of the sample, the fat-soluble substance contained in the sample is extracted into the supercritical carbon dioxide.

After extracting the fat-soluble substance, if supercritical carbon dioxide containing a small amount of co-solvent is again flowed through the sample remaining in the extraction container, components that were not extracted with pure supercritical carbon dioxide alone can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a cosolvent is additionally mixed with carbon dioxide.

As the co-solvent, one or a mixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether may be used. Preferably, one or a mixture of two or more selected from the group consisting of olive oil, ethanol, methanol, and water may be used.

Most of the extracted samples contain carbon dioxide, and since carbon dioxide is volatilized into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a vacuum evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration, and ultrasonic waves can destroy an insoluble solvent contained in a sample in an aqueous solvent, and the reactants located in the vicinity due to the high local temperature generated at this time. Because the kinetic energy of the particles is increased, sufficient energy for the reaction is obtained, and the high pressure is induced by the impact effect of ultrasonic energy to increase the mixing effect of the material and the solvent contained in the sample, thereby increasing the extraction efficiency.

The extraction solvent that can be used for the ultrasonic extraction method may be one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether. The extracted sample is vacuum filtered to recover the filtrate, then removed by a vacuum evaporator, and an extract can be obtained through a conventional extract preparation method of freeze-drying.

According to the present invention, the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition, preferably, 0.01 to 20 It is characterized in that it contains 0.1 to 10% by weight, more preferably 0.1 to 10% by weight. When the content of the extract is less than 0.001% by weight, the skin moisturizing effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin moisturizing effect for the content increase is insignificant, there is a problem in safety and stability of the formulation, and economical not even an enemy

The mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea according to the present invention contains quince, persimmon leaf, cinnamon bark, mugwort leaf, chrysanthelium indicum and green tea 1 to 3:1 It may be included in a mixed weight ratio of ~3:1 to 3:1 to 3:1 to 3:1 to 3, preferably in a mixed weight ratio of 1:1:1:1:1:1.

After removing unnecessary ingredients through a purification process in order to use the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea prepared in a conventional way in a cosmetic formulation, a cosmetic composition showing efficacy may include The purification process can be any conventional purification process, and liquid-liquid extraction using a difference in solubility, precipitation and a filter, and chromatography using the size of polarity and molecular weight, etc. can be used. Also, for example, after dissolving the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum and green tea in an ethanol aqueous solution (50%, 70% solution, etc.) or other aqueous solution, HP-20, sephadex Using a column packed with a column, the water-soluble material and the oil-soluble material are separated using the difference in polarity and molecular weight, and then the oil-soluble material is concentrated by TLC and freeze-dried to prepare a concentrate.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, conventional antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. adjuvants, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

In the case of cleansing in which the formulation of the present invention contains a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid as carrier components Amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in more detail through a preparation example. These preparation examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these preparation examples according to the gist of the present invention. .

Preparation Example 1 : Solvent mixture extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelum indicum and green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) were mixed with 60 g each, and then 70% (v) /v) reflux extraction 3 times for 5 hours each with an aqueous ethanol solution, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the mixed extract powder of dried quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 2 : Supercritical fluid mixture extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) are mixed with 60 g each, followed by a conventional supercritical Extraction was carried out using an extraction method (extraction using olive oil as a cosolvent in a supercritical state under a pressure of about 300 atm at a temperature of about 60°C), and after removing salts using an ultrafiltration membrane, concentrated under reduced pressure and freeze-dried. 31.3 g of dried quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea mixed extract powder were obtained. This was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 3 : Preparation of ultrasonic mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) are mixed with 60 g each, and then ultrasonically (supernonic) Extraction was performed using an ultrasonic device at 25 KHz at 30° C. for about 2 hours), and after removing salts using an ultrafiltration membrane, 7.1 g was obtained through concentration under reduced pressure and freeze-drying. Mixed extract powder of dried quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 4: Mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) 96g, 48g, 48g, 48g, 48g, respectively. After mixing 48 g each, this was extracted under reflux three times for 5 hours with 70% (v/v) aqueous ethanol solution, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the mixed extract powder of dried quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 5 : Mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) 48g, 96g, 48g, 48g, 48g, respectively, After mixing 48 g each, this was extracted under reflux three times for 5 hours with 70% (v/v) aqueous ethanol solution, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.9 g. Then, the mixed extract powder of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 6 : Mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) 48g, 48g, 96g, 48g, 48g, respectively, After mixing 48 g each, this was extracted under reflux three times for 5 hours with 70% (v/v) aqueous ethanol solution, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.6 g. Then, the mixed extract powder of dried quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 7 : Mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) 48g, 48g, 48g, 96g, 48g, respectively, After mixing 48 g each, this was extracted under reflux three times for 5 hours with 70% (v/v) aqueous ethanol solution, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the mixed extract powder of dried quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was used by dissolving it in 50% 1,3-butylene glycol.

Preparation 8 : quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, mixed extract of green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) 48g, 48g, 48g, 48g, 96g, respectively, After mixing 48 g each, this was extracted under reflux three times for 5 hours with 70% (v/v) aqueous ethanol solution, cooled, and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.9 g. Then, the mixed extract powder of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 9 : Mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelum indicum, green tea

Minced and dried quince (fruit), persimmon tree (leaf), broiler tree (bark), mugwort (leaf), chrysanthelium indicum (outpost) and green tea (leaf) 48g, 48g, 48g, 48g, 48g, respectively, After mixing 96 g each, this was extracted under reflux three times for 5 hours with 70% (v/v) aqueous ethanol solution, cooled and filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.6 g. Then, the mixed extract powder of dried quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea was used by dissolving it in 50% 1,3-butylene glycol.

Formulation Example : Preparation of a cosmetic composition containing a mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea

Cosmetics (Formulation Examples 1 to 9) each containing the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea of Preparation Examples 1 to 9 were prepared as in the composition of Table 1 below. In addition, for comparison of efficacy, a cosmetic composition containing glycerin and 1,3-butylene glycol as a moisturizer instead of a mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea (comparative formulation example) 1), a cosmetic composition (Comparative Formulation Example 2) that does not contain any extract or moisturizer was prepared as shown in Table 1 below.

Ingredients (unit: wt%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 Formulation Example 8 Formulation Example 9 Comparative Formulation Example 1 Comparative Formulation Example 2 Preparation Example 1 5.0 - - - - - - - - - - Preparation 2 - 5.0 - - - - - - - - - Preparation 3 - - 5.0 - - - - - - - - Preparation 4 - - - 5.0 - - - - - - - Preparation 5 - - - - 5.0 - - - - - - Preparation 6 - - - - - 5.0 - - - - - Preparation 7 - - - - - - 5.0 - - - - Preparation 8 - - - - - - - 5.0 - - - Preparation 9 - - - - - - - - 5.0 - - glycerin - - - - - - - - - 5.0 - 1.3 Butylene Glycol - - - - - - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 microcrystallin 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 squalane 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 liquid paraffin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 trioctanoin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 fragrance, preservative appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 1 : Measurement of skin electrical conductivity

The clinical moisturizing effect and lasting power of the cosmetic compositions of Formulation Examples 1 to 9 were measured as follows. Through the questionnaire, 220 healthy adult males and females who felt that their skin was dry were randomly formed into groups of 20, and Formulation Examples 1 to 9 and Comparative Formulation Examples 1 and 2 suitable for each group were administered twice a day for 4 weeks, respectively. was applied to both faces one by one. The moisturizing effect was evaluated by quantifying the increase rate (%) of the skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the actual use test, and the improvement effect after the end. The experimental results are shown in Table 2 below.

division before use 2 weeks after use 4 weeks after use Formulation Example 1 24.76 46.61 60.56 Formulation Example 2 24.65 42.98 45.75 Formulation Example 3 23.22 23.26 21.30 Formulation Example 4 25.28 38.79 52.22 Formulation Example 5 24.90 32.54 43.45 Formulation Example 6 23.55 26.26 25.16 Formulation Example 7 24.22 35.87 38.82 Formulation Example 8 25.68 38.66 54.99 Formulation Example 9 24.22 30.97 36.21 Comparative Formulation Example 1 23.26 23.17 24.16 Comparative Formulation Example 2 25.12 26.85 27.88

As shown in Table 2, as a comparative formulation, compared to cosmetics containing a chemical moisturizer (Comparative Formulation Examples 1 and 2), quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelum indicum, green tea of the present invention It was confirmed that the moisturizing effect of the cosmetics (Formulation Examples 1 to 9) containing the mixed extract of In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 9, since the efficacy of Formulation Example 1 is excellent, it was confirmed that it is most preferable to mix each component at a mixing ratio of 1:1:1:1:1:1. .

Experimental Example 2 : Skin barrier improvement effect

This study was conducted on 25 healthy women indoors at 25℃, 45% relative humidity, and no air flow. Skin barrier damage was induced by using a 1% aqueous solution of Sodium Lauryl Sulfate (SLS), and after applying the cosmetic compositions of Formulation Examples 1, 2, and 3 to the upper arm of the arm, TEWAMETER TM210 (C + K eletronic GmhH. Germany) was used to measure the amount of transdermal water loss. That is, the skin barrier recovery rate (%) was confirmed by measuring the TEWL (Transepidermal Water Loss) value according to the change in TEWL value over time. The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 62.78 81.98 92.22 98.45 Formulation Example 2 61.16 67.96 89.38 92.55 Formulation Example 3 58.82 67.45 83.73 85.06 Formulation Example 4 60.23 65.22 69.88 77.56 Formulation Example 5 57.52 58.88 71.23 76.14 Formulation Example 6 69.43 69.10 69.22 72.98 Formulation Example 7 58.49 62.44 71.21 81.32 Formulation Example 8 49.68 54.14 67.13 79.97 Formulation Example 9 51.55 53.59 61.87 67.62 Comparative Formulation Example 1 30.97 41.23 45.64 40.78 Comparative Formulation Example 2 41.55 50.62 62.74 61.74

As shown in Table 3, Formulation Examples 1 to 9 containing a mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea were quince, persimmon leaf, broiler bark, mugwort leaf, It can be seen that the skin barrier recovery rate was improved compared to Comparative Formulation Examples 1 and 2 that did not contain the mixed extract of Santellum indicum and green tea. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 9, it was confirmed that it is most preferable to mix at a mixing ratio of 1:1:1:1:1:1, since the efficacy of Formulation Example 1 is excellent.

Experimental Example 3 : Safety Test

A human skin patch test was performed to check whether the cosmetic compositions of Formulation Examples 1 to 9 were irritating to the skin. The test was conducted on 20 women in their 20s and 30s who have never had a hypersensitivity reaction to skin irritation in the past and who do not currently have skin diseases or skin allergies. Comparative Formulation Examples 1 and 2, which do not contain the mixed extract of green tea, were used as control examples.

First, the test site was wiped with 70% ethanol and dried. The prepared test material was dropped into the Finn chamber (100X10, EPITEST) and then sealed and applied to the back of the test subject. The patch was applied for 24 hours, and 1 hour and 24 hours after the patch was removed, the presence or absence of erythema and edema at the test site was observed using a magnifying glass (8MC-150, DAZOR).

The skin reaction was determined according to the regulations (Table 4) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 1 below, and the results are shown in Table 5.

sign score Evaluation standard - 0 no response ± 0.5 faint or mild erythema + One Demarcated but mild erythema, edema and papules ++ 2 Distinct erythema, papules and vesicles +++ 3 Severe erythema and blistering, crusting

division 24 hours 48 hours Average skin reaction
(n=20) ± + ++ ± + ++ Formulation Example 1 One - - - - - 0.11 Formulation Example 2 - - - - - - 0.00 Formulation Example 3 One - - - - - 0.14 Formulation Example 4 One - - One - - 0.00 Formulation Example 5 2 - - - - - 0.21 Formulation Example 6 2 - - - - - 0.12 Formulation Example 7 One - - One - - 0.16 Formulation Example 8 2 - - One - - 0.00 Formulation Example 9 One - - One - - 0.14 Comparative Formulation Example 1 2 - - - - - 0.28 Comparative Formulation Example 2 One - - One - - 0.29

As shown in Table 5, the cosmetic compositions of Formulation Examples 1 to 9 did not cause skin irritation. Therefore, cosmetic formulations containing mixed extracts of Gammoaceae, persimmon leaves, cinnamon bark, mugwort leaves, chrysanthelium indicum and green tea were added. It was found that there were no abnormalities in the safety of

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

Mixed extract of Chaenomeles sinensis fruit, Diospyros kaki leaf, Cinnamomum cassia bark, Artemisia princeps leaf, Chrysanthellum indicum and Green tea (Camellia sinensis leaf) A cosmetic composition for moisturizing the skin containing as an active ingredient. The cosmetic composition for skin moisturizing according to claim 1, wherein the cosmetic composition has an effect of improving skin barrier function. According to claim 1, wherein the quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthemum indicum, and the mixed extract of green tea is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition, the cosmetic composition for skin moisturizing. The method according to claim 1, wherein the mixed extract of quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, and green tea is (a) water, anhydrous or hydrous lower alcohol having 1-4 carbon atoms, propylene glycol, butylene A solvent extraction method using an extraction solvent selected from the group consisting of glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acids and mixtures thereof, (b) supercritical extraction, and (c) ultrasound A cosmetic composition for skin moisturizing, which is extracted by a method selected from the group consisting of extraction methods. According to claim 1, wherein the mixed extract is quince, persimmon leaf, broiler bark, mugwort leaf, chrysanthelium indicum, green tea 1~3:1~3:1~3:1~3:1~3:1~ A cosmetic composition for skin moisturizing, which is included in a mixed weight ratio of 3.