CN116869888A - Natural plant preservative composition with antibacterial effect and preparation and application thereof - Google Patents
Natural plant preservative composition with antibacterial effect and preparation and application thereof Download PDFInfo
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- CN116869888A CN116869888A CN202310972703.XA CN202310972703A CN116869888A CN 116869888 A CN116869888 A CN 116869888A CN 202310972703 A CN202310972703 A CN 202310972703A CN 116869888 A CN116869888 A CN 116869888A
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a natural plant preservative composition with a bacteriostatic effect, and a preparation method and application thereof, wherein the composition comprises the following components: rosemary extract, cynanchum atratum extract, weeping forsythiae extract, honeysuckle extract and hinoki extract. The composition can remarkably improve the antibacterial and antioxidant effects by fully exerting the synergistic effect among the extracts, mainly can inhibit 5 bacteria (escherichia coli, ATCC 8739, pseudomonas aeruginosa, ATCC 9027, staphylococcus aureus subspecies aureous, ATCC 6538, aspergillus brazilian, ATCC 16404, candida albicans and ATCC 10231) which are easy to pollute daily cosmetics, and can achieve the purpose of quick and long-acting bacteriostasis by mutual interaction.
Description
Technical Field
The invention relates to the field of cosmetic additives, in particular to a natural plant preservative composition with a bacteriostatic effect, a preparation method and application thereof in cosmetics.
Background
With the improvement of living standard, cosmetics become an indispensable part of life of people, and people use cosmetics more and more commonly, and more importance is attached to the safety of cosmetics. Most cosmetics are rich in nutrients required for microbial growth, and once the microbes in the environment enter, the microbes can quickly multiply, so that the sensory and quality of the product are destroyed, the health of consumers is damaged, and therefore, the inhibition of the propagation of the microbes in the cosmetics is important. The most common means for inhibiting the microbial proliferation in cosmetics is to add a preservative into the cosmetics, and 51 preservatives are used as the most commonly used preservatives in the current cosmetics according to the cosmetic safety technical Specification (2015 edition), wherein the parabens, the phenoxyethanol and the isothiazolinone are strong in sterilization capability, but after the cosmetics of the traditional chemical preservatives are used for a long time, the resident flora of the skin is damaged and inhibited, the microecological balance of the skin is disturbed, the skin barrier is damaged and the immunity is reduced, so that various skin problems are caused.
With the enhancement of health consciousness, people pay more attention to the green safety and functionality of cosmetics, and natural and healthy plant extract products are welcomed and favored by more and more consumers, and the market is expanding step by step. Thus, among the many types of bacteriostatic and preservative products today, bacteriostatic and preservative products derived from plant extracts are touted by numerous consumers due to their green, safety advantages.
The demands of preservatives are increasing, and the concepts of zero addition and no preservation are becoming more mainstream, so in order to meet the increasing demands of consumers, development of cosmetics without addition of chemical preservatives has become a hot spot.
Disclosure of Invention
The invention aims to provide a natural plant preservative composition with a bacteriostatic effect, all components of the composition are natural sources, the composition has a synergistic effect, the preservative effect of the composition can be greatly enhanced after compounding, and the composition has the advantages of low dosage, safety, effectiveness, natural non-irritation and the like, and can be used in the fields of cosmetics and the like.
Rosemary (Rosmarinus officinalis) is a dicotyledonous plant class, labiatae, rosemary evergreen shrub, mainly contains active ingredients such as rosmarinic acid, carnosol, carnosic acid, and the like, and has the effects of delaying aging, preserving, resisting bacteria, resisting oxidation, and the like.
The cynanchum atratum (Vincetoxicum atratum) is a vertical perennial herb of the genus Cynanchum of the family Apocynaceae and the class Magnoliaceae, mainly contains Bai Weisu, volatile oil, cardiac glycoside and the like, and has the effects of clearing heat, relieving swelling, promoting granulation, relieving pain, inducing diuresis, treating strangurtia, inhibiting bacteria and the like.
Fructus forsythiae (Forsythia suspensa) is leaf shrub of Oleaceae and Forsythia, and mainly contains active ingredients such as forsythol, forsythoside, oleanolic acid, and vitamin P, and has effects of clearing heat, detoxicating, resolving hard mass, relieving swelling, inhibiting bacteria, resisting inflammation, and resisting sensitization.
Flos Lonicerae (Lonicera japonica Thunb) is a semi-evergreen winding vine of Magnoliaceae, caprifoliaceae, and Lonicera, mainly contains polyphenols and chlorogenic acid, and has effects of clearing away heat and toxic materials, dispelling wind and heat, resisting bacteria, and resisting oxidation.
Cypress (Chamaecyparis Spach) is evergreen tree of Pinus, cupressaceae, and Platycladus, mainly contains phenolic compounds, alkaloids, and glycosides, and has effects of clearing heat, detoxicating, resisting allergy, resisting bacteria, diminishing inflammation, and resisting oxidation.
The technical scheme of the invention is as follows: the natural plant preservative composition with the bacteriostatic effect comprises the following components in parts by weight: 1-8 parts of rosemary extract, 0.5-12 parts of cynanchum atratum extract, 1-8 parts of fructus forsythiae extract, 0.1-5 parts of honeysuckle extract and 0.1-1 part of hinoki extract.
Preferably, the preservative composition comprises the following components in parts by weight: 3-6 parts of rosemary extract, 2.5-8 parts of cynanchum atratum extract, 3-6 parts of fructus forsythiae extract, 1.5-3.5 parts of honeysuckle extract and 0.1-1 part of hinoki extract.
More preferably, the preservative composition consists of the following components in parts by weight: 4-5 parts of rosemary extract, 5-6 parts of cynanchum atratum extract, 4-5 parts of fructus forsythiae extract, 1.9-2.9 parts of honeysuckle extract and 0.1-1 part of hinoki extract.
Another object of the present invention is to provide a method for preparing a natural plant preservative composition having a bacteriostatic effect, comprising the steps of:
(1) The preparation method of the rosemary extract comprises the following steps: pulverizing the dried traditional Chinese medicinal materials of rosemary, sieving with a 40-mesh sieve, extracting with distilled water as an extraction solvent for 2-3 h under reflux according to the proportion of 40-60 mL of extraction solvent per gram of rosemary, filtering, collecting filtrate, and evaporating the filtrate to obtain a crude extract; adding 90% ethanol into the crude extract, extracting according to the proportion of 10-60 mL of extraction solvent for each gram of crude extract, concentrating to remove ethanol, and obtaining rosemary extract;
(2) The preparation method of the cynanchum atratum extract comprises the following steps: pulverizing dried Chinese medicinal materials of cynanchum atratum, sieving with a 40-mesh sieve, reflux-extracting with 60% ethanol as an extraction solvent at 90 ℃ for 1-2 h according to the proportion of 15-20 mL of extraction solvent per gram of cynanchum atratum, centrifuging, filtering, collecting filtrate, and recording as primary filtrate; adding 90% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain radix Cynanchi Atrati extract;
(3) The preparation method of the fructus forsythiae extract comprises the following steps: pulverizing the dried traditional Chinese medicinal materials of fructus forsythiae, sieving with a 40-mesh sieve, extracting with 95% ethanol as an extraction solvent according to the ratio of 12-15 mL of the extraction solvent per gram of fructus forsythiae for 2-3 h, centrifuging, filtering, collecting filtrate, and recording as primary filtrate; adding 95% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain fructus forsythiae extract;
(4) The preparation method of the honeysuckle extract comprises the following steps: pulverizing dried traditional Chinese medicinal materials of honeysuckle, sieving with a 40-mesh sieve, taking ethanol with the concentration of 95% as an extraction solvent according to the proportion of 12-15 mL of the extraction solvent per gram of honeysuckle, ultrasonically extracting for 2-3 h, centrifuging, filtering, collecting filtrate, and recording as primary filtrate; adding 95% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain flos Lonicerae extract;
(5) The preparation method of the hinoki extract comprises the following steps: pulverizing dry Chinese medicinal materials of the phellodendron amurense, sieving with a 40-mesh sieve, taking 75% ethanol as an extraction solvent according to the proportion of 12-15 mL of the extraction solvent per gram of the phellodendron amurense, performing ultrasonic extraction for 2-3 h, centrifuging, filtering, collecting filtrate, and recording the filtrate as primary filtrate; adding 95% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain hinoki extract;
(6) Weighing rosemary extract, cynanchum atratum extract, fructus forsythiae extract, honeysuckle extract and hinoki extract according to the weight parts of the components in the anti-corrosion composition; mixing herba Rosmarini officinalis extract, radix Cynanchi Atrati extract, fructus forsythiae extract, flos Lonicerae extract, and herba Platycladi extract to obtain natural plant antiseptic composition.
It is another object of the present invention to provide the use of a natural plant preservative composition having a bacteriostatic effect for inhibiting the growth of escherichia coli, pseudomonas aeruginosa, staphylococcus aureus subspecies aureoviridis, aspergillus brasiliensis and/or candida albicans in cosmetics.
Another object of the present invention is to provide the use of a natural plant preservative composition having a bacteriostatic effect in the preparation of cosmetics.
Preferably, the natural plant preservative composition is added into the cosmetic in a weight ratio of 0.2% -2.0% of the cosmetic.
Preferably, the cosmetic is cream, gel, emulsion, essence, spray, toner, facial mask, facial cleanser, toning lotion, perfume, cleansing water, lipstick, blush, etc.
The natural plant preservative composition with the antibacterial effect comprises natural components extracted from plants, mainly comprises active components such as rosmarinic acid, flavonoid compounds, chlorogenic acid and the like, wherein the content of rosmarinic acid is 0.1-0.5 mg/mL, the content of flavonoid compounds is 1.5-2.0 mg/mL, and the content of chlorogenic acid is 0.6-3 mg/mL.
Advantageous effects
1. The plant preservative composition provided by the invention has the advantages of low dosage, safety, effectiveness, nature, no stimulation and the like, can be used in fields of cosmetics and the like with severe requirements on preservative components, and solves the preservative problem in the fields of cosmetics and the like;
2. the extract from the natural plant in step 5 is compounded, so that the synergistic effect is found, and the antibacterial and antioxidant effects can be obviously improved;
3. the invention provides a plant preservative composition with all components of natural sources, which can achieve the aim of rapid and long-acting bacteriostasis, can inhibit a plurality of bacteria such as escherichia coli ATCC 8739, pseudomonas aeruginosa ATCC 9027, staphylococcus aureus subspecies aureoja ATCC 6538, aspergillus brasiliensis ATCC 16404, candida albicans ATCC 10231 and the like, and has the advantage of wide antibacterial spectrum.
Detailed Description
The following detailed description of the embodiments of the present invention is provided for better illustration of the present invention, but is not to be construed as limiting the invention.
Preparation of a natural plant preservative composition:
1. preparation of the extract:
(1) The preparation method of the rosemary extract comprises the following steps: pulverizing dried herba Rosmarini officinalis, sieving with 40 mesh sieve, collecting 100g of herba Rosmarini officinalis powder, extracting with 5L distilled water under reflux for 3 hr, filtering, collecting filtrate, and evaporating the filtrate to obtain crude extract; adding 2.6L90% ethanol into the crude extract, concentrating to remove ethanol to obtain herba Rosmarini officinalis extract;
(2) The preparation method of the cynanchum atratum extract comprises the following steps: pulverizing dried Chinese medicinal materials of radix Cynanchi Atrati, sieving with 40 mesh sieve, collecting 100g of sieved radix Cynanchi Atrati powder, reflux-extracting with 2L of 60% ethanol as extraction solvent at 90deg.C for 2 hr, centrifuging, filtering, collecting filtrate, and recording as primary filtrate; adding 2L ethanol with 90% concentration into the filter residue, reflux-extracting at 90 ℃ for 2h, centrifuging, filtering, and collecting filtrate to obtain secondary filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain radix Cynanchi Atrati extract;
(3) The preparation method of the fructus forsythiae extract comprises the following steps: pulverizing dried fructus forsythiae Chinese medicinal materials, sieving with 40 mesh sieve, collecting 100g of sieved fructus forsythiae Chinese medicinal material powder, extracting with 1.5L95% ethanol as extraction solvent under ultrasonic wave for 2 hr, centrifuging, filtering, collecting filtrate, and recording as first filtrate; adding 1.5L of ethanol with the concentration of 95% into the filter residue, extracting by ultrasonic waves for 2 hours, centrifuging, filtering, and collecting filtrate to obtain secondary filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain fructus forsythiae extract;
(4) The preparation method of the honeysuckle extract comprises the following steps: pulverizing dried Chinese medicinal materials of flos Lonicerae, sieving with 40 mesh sieve, collecting 100g of sieved flos Lonicerae Chinese medicinal material powder, extracting with 1.2L95% ethanol as extraction solvent by ultrasonic wave for 2 hr, centrifuging, filtering, collecting filtrate, and recording as first filtrate; adding 1.2L ethanol with concentration of 95% into the filter residue, extracting with ultrasonic wave for 2h, centrifuging, filtering, and collecting filtrate to obtain secondary filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain flos Lonicerae extract;
(5) The preparation method of the hinoki extract comprises the following steps: pulverizing dry Chinese medicinal materials of hinoki wood, sieving with 40 mesh sieve, collecting 100g of Chinese medicinal materials of hinoki wood after sieving, extracting with 1.3L75% ethanol as extraction solvent under ultrasonic wave for 3 hr, centrifuging, filtering, collecting filtrate, and recording as first filtrate; adding 1.3L ethanol with concentration of 95% into the filter residue, extracting with ultrasonic wave for 3h, centrifuging, filtering, and collecting filtrate to obtain secondary filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain hinoki extract;
2. preparation of the composition:
composition 1:
weighing 40g of rosemary extract, 50g of cynanchum atratum extract, 40g of fructus forsythiae extract, 20g of honeysuckle extract and 5g of hinoki extract; mixing herba Rosmarini officinalis extract, radix Cynanchi Atrati extract, fructus forsythiae extract, flos Lonicerae extract, and herba Platycladi extract to obtain natural plant antiseptic composition.
Composition 2:
weighing 40g of rosemary extract, 60g of cynanchum atratum extract, 40g of fructus forsythiae extract, 25g of honeysuckle extract and 8g of hinoki extract; mixing herba Rosmarini officinalis extract, radix Cynanchi Atrati extract, fructus forsythiae extract, flos Lonicerae extract, and herba Platycladi extract to obtain natural plant antiseptic composition.
Composition 3:
weighing 40g of rosemary extract, 60g of cynanchum atratum extract, 50g of fructus forsythiae extract, 25g of honeysuckle extract and 10g of hinoki extract; mixing herba Rosmarini officinalis extract, radix Cynanchi Atrati extract, fructus forsythiae extract, flos Lonicerae extract, and herba Platycladi extract to obtain natural plant antiseptic composition.
The bacteriostasis experiment process comprises the following steps:
experimental materials:
bacteria: escherichia coli (Escherichia coli), ATCC 8739; pseudomonas aeruginosa (Pseudomonas aeruginosa), ATCC 9027; staphylococcus aureus (Staphylococcus aureus), ATCC 6538;
fungi: candida albicans (Candida albicans), ATCC 10231; aspergillus niger (Aspergillus brasiliensis), ATCC 16404;
experimental reagent: nutrient agar (Guangdong Cryptographic microorganism Co., ltd.); deionized water of potato dextrose agar medium (Guangdong Crypton microorganism technology Co., ltd., 027130) and sterilizing for use; 0.85% physiological saline and sterilizing for standby.
1. Bacterial suspension preparation
Respectively collecting 1 strain of 3 bacterial inclined plane strains, respectively washing inclined plane lawn with 15ml sterilized 0.85% physiological saline 3 times in 100ml silk mouth reagent bottle to obtain 10 strains 7 ～10 8 cfu/ml bacterial suspension, respectively selecting 3 strains with concentration of 10 7 ～10 8 cfu/ml strain liquid is mixed in a 100ml silk mouth reagent bottle as an equal volume and stored at 4 ℃ for standby.
2. Preparation of fungal suspensions
Candida albicans: taking 1 strain of candida albicans inclined plane strain, washing inclined plane lawn with 15ml sterilized 0.85% physiological saline for 3 times in a 100ml screw mouth reagent bottle to obtain 10 7 ～10 8 cfu/ml bacterial suspension is preserved at 4 ℃ for standby.
Aspergillus niger: taking 1 strain of Aspergillus niger inclined plane strain, sterilizing 15ml with 0.85% physiological saline containing 0.05% Tween, washing inclined plane lawn 3 times in 100ml silk mouth reagent bottle, and making into 10 7 ～10 8 cfu/ml bacterial suspension, respectively selecting Aspergillus niger and candida albicans bacterial strain with concentration of 10 7 ～10 8 cfu/ml strain liquid is mixed in a 100ml silk mouth reagent bottle as an equal volume and stored at 4 ℃ for standby.
3. Working strain concentration test:
shaking 0.5mL of working strain in 49.5mL of sterilized normal saline, adding 1mL into a 99mL sterilized normal saline conical flask, and mixing thoroughly to obtain 1:10 4 And (5) detecting liquid. Continue to dilute to 1:10 5 ，1:10 6 …1:10 8 1 pipette should be replaced for each dilution, the liquid to be tested with the dilution of 5-8 is generally cultured, and the liquid to be tested is counted according to colony counting method in cosmetic safety technical Specification (2015) after culture, and the concentration of the bacterial mixed suspension and the fungal mixed suspension is selected to be about 10 7 ～10 8 cfu/ml, and finally obtaining the concentration of the bacterial mixed suspension and the fungal mixed suspension for sample inoculation.
4. Sample preparation and inoculation:
taking 1 part of packaged sample to be tested, weighing 50g in 2 parts of sterilizing reagent bottles respectively under aseptic conditions for standby, selecting proper bacteria solution concentration, taking 2 parts of the sample to be tested with the sample weighing amount of 50g, adding 0.5mL of mixed bacterial suspension into one part, adding 0.5mL of mixed fungus suspension into the other part, uniformly mixing the liquid by a vortex oscillator, stirring and uniformly mixing the paste by a sterilizing spoon, storing the sample at room temperature (22.5+/-2.5 ℃), and respectively culturing and counting for 7, 14, 21 and 28 days, and counting according to related requirements.
Bacteriostasis test:
antibacterial effect of single extract:
table 1: results of bacterial inhibition by single extract
Table 2: results of single extract on inhibition of fungi
As can be seen from the table above, the extract of cynanchum atratum has the greatest influence on the total number of bacteria, and the other extracts are rosemary extract, weeping forsythiae capsule extract, hinoki extract and honeysuckle extract in turn; the extracts of Cynanchum atratum, cypress, flos Lonicerae, herba Rosmarini officinalis and flos Lonicerae have the greatest influence on total fungi;
method challenge test was performed after combining the extracts according to different ratios as listed above
Test results:
table 3: results of inhibition of bacteria by the composition
Table 4: results of the composition on inhibition of fungi
As can be seen from the table, the composition has great improvement on the inhibition effect on bacteria and fungi, and the inhibition effect is improved by more than 100 times, so that the natural extract has good synergistic effect.
It is apparent that the above examples are only illustrative of the present invention and are not limiting of the embodiments of the present invention. Various modifications and alterations of this invention may be made by those skilled in the art without departing from the spirit and scope of this invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (8)
1. The natural plant preservative composition with the antibacterial effect is characterized by comprising the following components in parts by weight: 1-8 parts of rosemary extract, 0.5-12 parts of cynanchum atratum extract, 1-8 parts of fructus forsythiae extract, 0.1-5 parts of honeysuckle extract and 0.1-1 part of hinoki extract.
2. The natural plant preservative composition with bacteriostatic effect according to claim 1, characterized in that it consists of the following components in parts by weight: 3-6 parts of rosemary extract, 2.5-8 parts of cynanchum atratum extract, 3-6 parts of fructus forsythiae extract, 1.5-3.5 parts of honeysuckle extract and 0.1-1 part of hinoki extract.
3. The natural plant preservative composition with bacteriostatic effect according to claim 1, characterized in that it consists of the following components in parts by weight: 4-5 parts of rosemary extract, 5-6 parts of cynanchum atratum extract, 4-5 parts of fructus forsythiae extract, 1.9-2.9 parts of honeysuckle extract and 0.1-1 part of hinoki extract.
4. The method for preparing a natural plant preservative composition with bacteriostatic effect according to claim 1, which is characterized by comprising the following steps:
(1) The preparation method of the rosemary extract comprises the following steps: pulverizing the dried traditional Chinese medicinal materials of rosemary, sieving with a 40-mesh sieve, extracting with distilled water as an extraction solvent for 2-3 h under reflux according to the proportion of 40-60 mL of extraction solvent per gram of rosemary, filtering, collecting filtrate, and evaporating the filtrate to obtain a crude extract; adding 90% ethanol into the crude extract, extracting according to the proportion of 10-60 mL of extraction solvent for each gram of crude extract, concentrating to remove ethanol, and obtaining rosemary extract;
(2) The preparation method of the cynanchum atratum extract comprises the following steps: pulverizing dried Chinese medicinal materials of cynanchum atratum, sieving with a 40-mesh sieve, reflux-extracting with 60% ethanol as an extraction solvent at 90 ℃ for 1-2 h according to the proportion of 15-20 mL of extraction solvent per gram of cynanchum atratum, centrifuging, filtering, collecting filtrate, and recording as primary filtrate; adding 90% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain radix Cynanchi Atrati extract;
(3) The preparation method of the fructus forsythiae extract comprises the following steps: pulverizing the dried traditional Chinese medicinal materials of fructus forsythiae, sieving with a 40-mesh sieve, extracting with 95% ethanol as an extraction solvent according to the ratio of 12-15 mL of the extraction solvent per gram of fructus forsythiae for 2-3 h, centrifuging, filtering, collecting filtrate, and recording as primary filtrate; adding 95% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain fructus forsythiae extract;
(4) The preparation method of the honeysuckle extract comprises the following steps: pulverizing dried traditional Chinese medicinal materials of honeysuckle, sieving with a 40-mesh sieve, taking ethanol with the concentration of 95% as an extraction solvent according to the proportion of 12-15 mL of the extraction solvent per gram of honeysuckle, ultrasonically extracting for 2-3 h, centrifuging, filtering, collecting filtrate, and recording as primary filtrate; adding 95% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain flos Lonicerae extract;
(5) The preparation method of the hinoki extract comprises the following steps: pulverizing dry Chinese medicinal materials of the phellodendron amurense, sieving with a 40-mesh sieve, taking 75% ethanol as an extraction solvent according to the proportion of 12-15 mL of the extraction solvent per gram of the phellodendron amurense, performing ultrasonic extraction for 2-3 h, centrifuging, filtering, collecting filtrate, and recording the filtrate as primary filtrate; adding 95% ethanol with the same volume as the first filtrate to the filter residue, and extracting again to obtain a second filtrate by the same method as the first filtrate; combining the first filtrate and the second filtrate, concentrating to remove ethanol to obtain hinoki extract;
(6) Weighing rosemary extract, cynanchum atratum extract, fructus forsythiae extract, honeysuckle extract and hinoki extract according to the weight parts of the components in the anti-corrosion composition; mixing herba Rosmarini officinalis extract, radix Cynanchi Atrati extract, fructus forsythiae extract, flos Lonicerae extract, and herba Platycladi extract to obtain natural plant antiseptic composition.
5. Use of a natural plant preservative composition having a bacteriostatic effect according to claims 1-3 for inhibiting the growth of escherichia coli, pseudomonas aeruginosa, staphylococcus aureus subspecies aureoviridis, aspergillus brasiliensis and/or candida albicans in cosmetics.
6. Use of a natural plant preservative composition having a bacteriostatic effect according to claims 1-3 for the preparation of cosmetics.
7. The use according to claim 5 or 6, wherein the natural plant preservative composition is added to the cosmetic in a weight proportion of 0.2 to 2.0% based on the cosmetic.
8. The use according to claim 5 or 6, wherein the cosmetic is a cream, a gel, an emulsion, a serum, a spray, a toner, a mask, a cleanser, a lotion, a perfume, a makeup remover, a lipstick, a blush, or the like.
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| KR20150139126A (en) * | 2014-06-02 | 2015-12-11 | 청운대학교산학협력단 | Cosmetics containing artemisia annua |
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