CN103767980A - Natural plant compound prescription with preservative effect - Google Patents
Natural plant compound prescription with preservative effect Download PDFInfo
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- CN103767980A CN103767980A CN201410053987.3A CN201410053987A CN103767980A CN 103767980 A CN103767980 A CN 103767980A CN 201410053987 A CN201410053987 A CN 201410053987A CN 103767980 A CN103767980 A CN 103767980A
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Abstract
The invention relates to a natural plant extract compound prescription with preservative and antimicrobials, which comprises wrinkled gianthyssop, basil and rosemary. The plant compound prescription has the preservative effect, and can substitute chemical preservatives to lower the toxic or side effect of the chemical preservatives.
Description
Technical field
The invention belongs to natural antiseptic agent field, relate to one group of natural plants compound extract with antiseptic effect, particularly relating to one group is the natural plants compound recipe that can be used as cosmetics preservative with preservative effectiveness of raw material with Herba Pogostemonis, Herba Ocimi (Herba Ocimi Pilosi), Herba Rosmarini Officinalis.
Background technology
Antibiotic antiseptic the earliest comes from nature, but the restriction of the problem such as action effect is poor, shortage of resources is replaced by chemosynthesis antibiotic antiseptic gradually owing to being subject to.Along with the mankind are healthy and environmental issue is familiar with improves constantly to self, people find that many chemosynthesis antibacterial have potential carcinogenic, teratogenesis and i.e. " three cause " effect of mutagenic action gradually, and the exploitation of new chemical synthetic antimicrobial antiseptic is more and more difficult, make natural antibiotic antiseptic be subject to once again people's favor.Except take natural antimicrobial substance as raw material or model compound carry out the exploitation of chemical antibiotic antiseptic, also have many natural materials can directly be used as antibiotic antiseptic.Natural antibiotic antiseptic can be divided into plant-source antibacterial antiseptic, animal sources antibiotic antiseptic and microbial source antibiotic antiseptic by its source.If from chemical composition, natural antibiotic antiseptic can be divided into low molecule antibiotic antiseptic and high-molecular anti-bacteria antiseptic.The former is mainly alkaloid, organic acid, phenols and volatilization wet goods; Latter is mainly tannin, polysaccharide and protein.
According to incompletely statistics, the current plant that can be used as in the world antibiotic antiseptic has 2000 kinds of left and right at least.These plants all get a good chance of becoming the resource of antibiotic antiseptic exploitation.The feature of plant-source antibacterial antiseptic is that toxicity is low, source is abundant and cheap.
The chemical composition of plant source antiseptic agent is very complicated, this is main because biology forms and accumulated the unequal secondary metabolite of content in growth course, wherein a lot of chemical compositions all have important physiologically active, as glycoside, alkaloids, terpenoid and volatilization wet goods.The researchs such as Robert A show, the inhibitor that suppresses Aspergillus flavus is the cattle thing bases such as the phenylpropyl compounds such as flavone, flavonol, coumarin, chromone and flavonoid (polyphenol), terpenoid, caffeine, Fructus Piperis powder.Wu Chuanwan etc. describe the structure type of Natural Antibacterial Constituents from Plant Origin in detail, as terpenoid, alkaloids, flavonoid, glycoside, Saponin, coumarin and lignin, esters, aldehydes, phenols and alcohols, organic acid, quintessence oil class, Saponin, steroid class, stilbene class, Anthraquinones etc.The researchs such as Tao Yongxia show, tomato biological alkali has stronger inhibitory action to Putrefying bacteria common in food, yeast, and to the inhibitory action of mycete a little less than, flavone compound particularly isoflavone compounds also has very strong antibacterial action.The reports such as Yang Lin, the tannin in Pericarpium Granati and flavone compound have certain bacteriostasis.Yan Zankai etc. isolate hesperidin from Pericarpium Citri tangerinae, and it all has inhibitory action to microorganism common in food.The researchs such as Cheng Zhen show, the bacteriostatic active ingredients of seeds of Urtica cannabina is mainly phenolic compound, Coumarins and organic acid etc.The researchs such as Wang Hang show, effective antipathogenic composition of Radix Sophorae Flavescentis is the material that contains alkaloid, aldehydes matter and flavonoid, and effective antipathogenic composition of Radix Sanguisorbae is alkaloid and Flavonoid substances. and the main matter that plays bacteriostasis in Borneolum Syntheticum is quinones.Plants essential oil (as Adeps Bovis seu Bubali, Flos Caryophylli, Cortex Cinnamomi, Herba thymi vulgaris, Herba Menthae, Herba Rosmarini Officinalis, Herba Coriandri, Salvia japonica Thunb. etc.) and its one pack system (as eugenol, carvacrol, cinnamic acid, hexanal, thymol, carvone, cinnamic aldehyde, citral, geraniol etc.) food-borne bacterium is had to very strong inhibitory action: the contained complex chemical composition of plants essential oil, its chemical constitution can be divided into aliphatic, aromatic series and terpenoid three major types compound and its containing oxygen derivative as alcohol, aldehyde, ketone, acid, ether, ester, lactone etc., also has in addition nitrogenous and compound sulfur-bearing.Thin elegant woods etc. has been studied the antibacterial action of plants essential oil and the application in food industry thereof.
Herba Pogostemonis is Labiatae herbaceos perennial, and Herba Pogostemonis is containing volatile oil 0.28%, and main component is that estragole (methylchavicol) accounts for more than 80%.And contain anethole (anethole), anisaldehyde (anisaldehyde), limonene (limonene), methoxy cinnamaldehyde (P-methoxycinnamaldehyde), α-and-nopinene (pinene), 3-octanone (3-octanone), 1-OCOL (1-octen-3-ol), linalool (linalool), 1-Flos Caryophylli alkene (1-carypholene), beta-elemene (β-elemene), β-Humuleno (β-humu-lene), ylangene (α-farnesene (β-farnesene), γ-Cadinene (γ-cadinene), Rhizoma Acori Graminei alkene (calamenene), also contain cis-beta, gamma-olefine aldehydr (cis-beta, gamma-hexenal).Flavone compound: acacetin (acacetin), tilia element (tilianin), linarin (linarin), agastachoside (agastachoside), different agastachoside (isoagastachoside), Herba Pogostemonis essence (agastachin).Root is Crataegolic acid (crateglic acid) containing maslinic acid (mas-linic acid), oleanolic acid (oleanolic acid), 3-O-acetyl group oleanolic aldehyde (3-O-acetyl oleanolic aldehyde), acacetin, tilia element, agastachoside, daucosterol (daucosterol), cupreol (β-sitosterol), Dehydroagastol (dehydro-agastol), 1-methylene-6, 8-dihydroxy-5-methoxyl group-7-(1, 1-dimethyl methylol)-1, 2, 3, 4, 9, 10, 10, a-seven hydrogen-9-phenanthrenone [1-methylene-2, 4a-dimethyl-6, 8-dihydroxy-5-methoxy-7-(1, 1-dimethyl-hydroxy methyl)-1, 2, 3, 4, 10, 10a-heptahydro-9-phenanthroxe].
Experimental results show that, Herba Pogostemonis decoct (8%～15%) has inhibitory action to multiple pathogenic funguses such as trichophyton in vitro. and ether leachate, alcohol extract, water-leach liquor also can suppress multiple pathogenic fungus. and test shows, Herba Pogostemonis ether leachate, alcohol extract, water-leach liquor and decoct are respectively 3%, 5%, 10%, 15% to trichophyton interdigitale and the trichophytic Mlc of the sufficient sole of the foot, and the antibacterial ability of prompting leachate is strong than decoct.
Herba Ocimi (Herba Ocimi Pilosi) (Ocimun basilicum L.), English Basil by name, is Labiatae basil, draft, with all herbal medicine, is famous medicine-food two-purpose fragrant plant.It is warm in nature hides pungently, has dispelling wind circulation of qi promoting, the function that removing dampness helps digestion, invigorates blood circulation, detoxifies.For headache due to invasion of exogenous pathogens, the flatulence due to improper diet stagnation of QI, gastralgia, have loose bowels, the treatment of the disease such as menoxenia, traumatic injury, snake bite and insect sting, skin eczema.Some prune a little beautiful potted landscape, can potted plantly view and admire.Herb is small and exquisite, leaf color jade green, and pattern is bright-coloured, and sweet perfumes are diffused all around, and have the effect of expelling mosquito.
Leaf of Herba Rosmarini Officinalis is with tea perfume (or spice), and peppery, the micro-hardship of acrid in the mouth, is often used in culinary art upper, also can be used to steep Herb Tea and drinks.Evergreen shrubs, thinks Herba Rosmarini Officinalis energy hypermnesis ancient times, and the antioxidant content in the plant rosemary that possesses Wheat Protein most of generally acknowledging is at present mainly the compositions such as carnosic acid, carnosol, rosmanol, ursolic acid, rosmarinic acid.Research shows, rosmarinic acid all has antibacterial activity to escherichia coli and staphylococcus aureus, and the activity of staphylococcus aureus is greater than colibacillary activity; Rosmarinic acid can change the permeability of bacterial cell membrane, causes the seepage of reducing sugar and protein and affects cellular metabolism, affects DNA replication dna, thereby brought into play bacteriostasis by the activity that suppresses archaeal dna polymerase.
Summary of the invention
The object of the present invention is to provide one group of natural plants compound extract with antiseptic effect, particularly relating to one group is the natural plants compound recipe that can be used as cosmetics preservative with preservative effectiveness of raw material with Herba Pogostemonis, Herba Ocimi (Herba Ocimi Pilosi), Herba Rosmarini Officinalis.
In order to realize foregoing invention object, the technical solution adopted in the present invention is: by existing extractive technique, adopt water and organic solvent extraction, extraction temperature is 50-99 ℃, and extraction time is 1-8h, and extracting solvent ratios is 6-10 times of crude drug quality.Wherein, optimum extraction mode is 50-95wt% ethanol extraction, and optimum extraction temperature is 80-99 ℃, and the optimum extraction time is 2-4h, and optimum extraction solvent ratios is 6-10 times of crude drug quality.After mixing with variable concentrations proportioning and crude drug after plant compound extract extracts separately by various crude drugs, extract again two kinds of mode systems.The plant compound extract making comprises Herba Pogostemonis 0.33-99wt%, Herba Ocimi (Herba Ocimi Pilosi) 0.33-99wt%, and Herba Rosmarini Officinalis 0.33～99wt%, wherein optimum combination is Herba Pogostemonis 10-80wt%, Herba Ocimi (Herba Ocimi Pilosi) 10-80wt%, Herba Rosmarini Officinalis 10-80wt%.Natural plants compound extract of the present invention can also comprise the plants such as Salvia japonica Thunb., Flos Lonicerae, Radix Salviae Miltiorrhizae, Radix Scutellariae.
The invention has the advantages that: (1) provides one group of natural anticorrosion plant compound recipe, in order to instead of chemical antiseptic, this invention can solve " three cause " problem of chemical preservative, has effect safer, low stimulation; (2) provide one group of natural anticorrosion plant compound recipe, solve the problem that single plant prescription is difficult to possess natural plants antiseptic effect, by composite and synergism, the formula of this invention has the effect that suppresses staphylococcus aureus, escherichia coli, bacillus pyocyaneus, candida albicans and aspergillus niger; Natural anticorrosion plant formula of the present invention is added in daily chemical products by a certain percentage, well instead of chemical antiseptic, and there is good antiseptic effect.
The specific embodiment
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.
Embodiment mono-: the preparation of natural anticorrosion plant compound recipe 1
Get Herba Pogostemonis 80g, Herba Ocimi (Herba Ocimi Pilosi) 10g, Herba Rosmarini Officinalis 10g, mix and pulverized after, pack in the round-bottomed flask of 2L, adding 1L volume fraction is 75% alcoholic solution, put into electric jacket 70 ℃ of reflux, extract, 5 hours, extracting solution is concentrated to 30g through Rotary Evaporators, adds propylene glycol 70g, solvent components does not filter, and makes plant compound recipe 1.
Embodiment bis-: the preparation of natural anticorrosion plant compound recipe 2
Get Herba Pogostemonis 50g, Herba Ocimi (Herba Ocimi Pilosi) 30g, Herba Rosmarini Officinalis 20g, mix and pulverized after, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 0.5L is 50%, puts into electric jacket 80 ℃ of reflux, extract, 3 hours, and extracting solution is concentrated to 60g through Rotary Evaporators, add 1,3-butanediol 40g, solvent components does not filter, and makes plant compound recipe 2.
Embodiment tri-: the preparation of natural anticorrosion plant compound recipe 3
Get Herba Pogostemonis 10g, Herba Ocimi (Herba Ocimi Pilosi) 80g, Herba Rosmarini Officinalis 10g, mix and pulverized after, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 0.8L is 95%, put into electric jacket 95 ℃ of reflux, extract, 3 hours, extracting solution is concentrated to 80g through Rotary Evaporators, adds glycerol 20g, solvent components does not filter, and makes plant compound recipe 3.
Embodiment tetra-: the preparation of natural anticorrosion plant compound recipe 4
Get Herba Pogostemonis 10g, Herba Ocimi (Herba Ocimi Pilosi) 10g, Herba Rosmarini Officinalis 80g, mix and pulverized after, pack in the round-bottomed flask of 3L, the alcoholic solution that the volume fraction that adds 2L is 75%, put into electric jacket 80 ℃ of reflux, extract, 8 hours, extracting solution is concentrated to 60g through Rotary Evaporators, adds ethanol 40g, solvent components does not filter, and makes plant compound recipe 4.
Embodiment five: the preparation of natural anticorrosion plant compound recipe 5
Get Herba Pogostemonis 10g, Herba Ocimi (Herba Ocimi Pilosi) 50g, Herba Rosmarini Officinalis 40g, mix and pulverized after, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 1.5L is 50%, put into electric jacket 90 ℃ of reflux, extract, 3 hours, extracting solution is concentrated to 50g through Rotary Evaporators, adds glycerol 50g, solvent components does not filter, and makes plant compound recipe 5.
Embodiment six: the preparation of natural anticorrosion plant compound recipe 6
Get Herba Pogostemonis 100g, after medicine is pulverized, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 0.8L is 95%, puts into electric jacket 75 ℃ of reflux, extract, 3 hours, and extracting solution is concentrated to 50g through Rotary Evaporators; Get Herba Ocimi (Herba Ocimi Pilosi) 100g, after medicine is pulverized, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 1.0L is 80%, puts into electric jacket 95 ℃ of reflux, extract, 5 hours, and extracting solution is concentrated to 50g through Rotary Evaporators; Get Herba Rosmarini Officinalis 100g, after medicine is pulverized, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 1.2L is 50%, puts into electric jacket 80 ℃ of reflux, extract, 8 hours, and extracting solution is concentrated to 50g through Rotary Evaporators; By three kinds of extracting solution in mass ratio 1:1:1 mix, then add the propylene glycol of same volume, solvent components does not filter, and makes plant compound recipe 6.
Embodiment seven: the preparation of natural anticorrosion plant compound recipe 7
Get Herba Pogostemonis 35g, Herba Ocimi (Herba Ocimi Pilosi) 35g, Herba Rosmarini Officinalis 30g, after medicine is pulverized, packs in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 0.8L is 75%, put into electric jacket 85 ℃ of reflux, extract, 3 hours, extracting solution is concentrated to 50g through Rotary Evaporators, adds glycerol 50g, solvent components does not filter, and makes plant compound recipe 7.
Effect embodiment: MIC pH-value determination pH
1 test strain
Staphylococcus aureus (ATCC8739), escherichia coli (ATCC6538), bacillus pyocyaneus (ATCC9027), candida albicans (ATCC10231), Aspergillus niger (ATCC16404).
2 culture medium
(1) TSB solid medium: peptone 10g/L, NaCl10g/L, K2HPO42.5g/L, yeast extract 3g/L, agar 15g/L, distilled water dissolves, and regulating pH is 7.2 ± 0.2,115 ℃ of autoclaving 20min; TSB fluid medium: peptone 10g/L, NaCl10g/L, K2HPO42.5g/L, yeast extract 3g/L, distilled water dissolves, and regulating pH is 7.2 ± 0.2,115 ℃ of autoclaving 20min;
(2) SDB solid medium: peptone 10g/L, glucose 20g/L, K2HPO43g/L, yeast extract 5g/L, agar 15g/L, distilled water dissolves, 115 ℃ of autoclaving 20min; SDB fluid medium: peptone 10g/L, glucose 20g/L, K2HPO43g/L, yeast extract 5g/L, distilled water dissolves, 115 ℃ of autoclaving 20min.
3. collecting cells and inoculation
(1) get the staphylococcus aureus of glycerol preservation, escherichia coli, bacillus pyocyaneus 100 μ L coat on TSB solid medium, cultivate 2d for 37 ℃, resuspended with normal saline, obtain bacteria suspension, and with plating method counting, test bacteria concentration are adjusted into 10
5～10
6cFU/mL.
(2) candida albicans and Aspergillus niger 100 μ L coat on SDB solid medium, cultivate 2d for 30 ℃, resuspended with normal saline, obtain bacteria suspension, and with plating method counting, test bacteria concentration are adjusted into 10
5～10
6.
The mensuration of 4 minimum inhibitory concentrations (MIC value)
Testing sample plant compound recipe 1-7 is become to variable concentrations with Herba Pogostemonis extracting solution, Herba Ocimi (Herba Ocimi Pilosi) extracting solution, Herba Rosmarini Officinalis extracting solution with normal saline dilution.With 2 × TSB or 2 × SDB fluid medium dilution bacteria suspension, making its final bacteria concentration is 10
5cFU/mL.In micropore, add bacterium liquid 100 μ L and medicinal liquid 100 μ L, establish the normal growth contrast that does not add the negative control of bacterium and do not add medicinal liquid simultaneously, every kind of medicine do 3 parallel, average.Put 37 ℃ or 30 ℃ of wet boxes and hatch, observed result after 48h, adopts direct method reading out data.The prerequisite of result judgement is that growth control is good, and blank asepsis growth is clear, and with the rising of drug level gradient, the growth of bacterium is suppressed in other hole.
5 results
It is anticorrosion that Application Example 1 is applied to cream frost
Cream frost formula
Preparation technology:
1. first hyaluronate sodium is dispersed in glycerol, then adds dissipation of heat (85 ℃), after being uniformly dispersed, add other components of B phase to stir; Mutually each A component is fully uniformly mixed in 85 ℃; C is fully dissolved and stirred mutually; C is added to A and stirs in mutually, then join together B mutually in, homogenizing stirs 4 minutes; The complete stirring froth breaking of homogenizing, and cooling; Add the mutually each component of D in 45 ℃, discharging after stirring, pH is controlled at 5.5-7.0.
2. preservation challenge experiment:
2.1 test strain
Staphylococcus aureus (ATCC8739), escherichia coli (ATCC6538), bacillus pyocyaneus (ATCC9027), candida albicans (ATCC10231), Aspergillus niger (ATCC16404).
2.2 culture medium
TSB solid medium: peptone 10g/L, NaCl10g/L, K
2hPO
42.5g/L, yeast extract 3g/L, agar 15g/L, distilled water dissolves, and regulating pH is 7.2 ± 0.2,115 ℃ of autoclaving 20min.SDB solid medium: peptone 10g/L, glucose 20g/L, K
2hPO
43g/L, yeast extract 5g/L, agar 15g/L, distilled water dissolves, 115 ℃ of autoclaving 20min.Lecithin tween 80 nutrient agar culture medium, is purchased from traditional Chinese medicines chemical reagent.Testing sample need be beforehand with microbial count and detect, and preservation challenge must be aseptic with sample.
2.3 preparations for sample product
Fluid sample: water miscible fluid sample, can measure 10mL and be added in 90mL sterile saline, as sample is less than 10mL, still undertaken by 10 times of dilution methods.As being added to 45mL sterile saline for 5mL, after mixing, make 1:10 inspection liquid.
Oil-based liquid sample, sample thief 10mL, first adding 5mL sterilized liquid paraffin mixes, add again the Tween 80 of 10mL sterilizing, in 40 ℃～44 ℃ water-baths, vibration mixes 10min, the normal saline 75mL(that adds sterilizing is pre-temperature in 40 ℃～44 ℃ water-baths), emulsifying in 40 ℃～44 ℃ water-baths, makes the suspension of 1:10.
Cream, frost, Emulsion semi-solid sample: hydrophilic sample: take 10g, be added in the triangular flask that bead and 90mL sterile saline are housed, fully vibration mixes, and leaves standstill 15min.Inspection liquid with its supernatant as 1:10.Hydrophobicity sample: take 10g, be put in the mortar of sterilizing, add 10mL sterilized liquid paraffin, grind to form thickly, then add 10mL sterilizing Tween 80, grind to be dissolved after, add 70mL sterile saline, in 40 ℃～44 ℃ water-baths, fully mix, make 1:10 inspection liquid.
Solid sample: take 10g, be added in 90mL sterile saline, fully vibration mixes, and makes it disperse suspendible, after leaving standstill, gets the inspection liquid of supernatant as 1:10.
If any homogenizer, above-mentioned water solublity cream, frost, powder etc., can claim 10g sample to add 90mL sterile saline, homogenizing 1min～2min; Hydrophobicity cream, frost and eyebrow pencil, lipstick etc., claim 10g sample, adds 10mL sterilized liquid paraffin, 10mL Tween 80,70mL sterile saline, homogenizing 3min～5min.
2.4 operating procedure
According to concentration of preservatives, 1:10 can be examined to liquid and be diluted to again 1:100,1:1000 ... Deng, eliminate the effect of antiseptic.Draw inspection liquid 2mL, be injected into respectively in two sterilizing plates every ware 1mL.
Be poured in plate melting and being chilled to the lecithin tween 80 nutrient agar culture medium of 45～50 ℃, the about 15mL of every ware, rotates plate immediately, sample is fully mixed homogeneously with culture medium, after agar solidifies, upset plate, puts cultivation 48h ± 2h in 36 ℃ ± 1 ℃ incubator.Separately get an empty plate of sterilizing that does not add sample, add about 15mL lecithin tween 80 nutrient agar culture medium, after agar solidifies, upset plate, puts in 36 ℃ ± 1 ℃ incubator and cultivates 48h ± 2h, is blank.
Mode and the quantity of 2.5 preservation challenge inoculations
Adopt single bacterium inoculation, get 5ml testing sample in test tube, accessing respectively staphylococcus aureus, escherichia coli and bacillus pyocyaneus is 1 × 10 to inoculating quantity
6cFU/g or 1 × 10
6cFU/ml; Candida albicans and Aspergillus niger inoculation quantity are 1 × 10
4cFU/g or 1 × 10
4cFU/ml.Every kind of bacterium each three parallel.
2.6 preservation challenge separation detection
Postvaccinal sample is in special time separation detection: 0 hour (after inoculation, stirring and evenly mixing, at once sampling), 2 days, 7 days, 14 days and 28 days.Operating procedure: first prepare TSB and SDB culture medium flat plate; After sampling, according to experiment needs, with 10 times, 100 times of normal saline dilutions and 1000 times etc., get 100 μ L and coat TSB and SDB culture medium flat plate, 28 ℃ ± 1 ℃ or 36 ℃ ± 1 ℃ cultivation; Colony counting.
The efficiency evaluation standard of 2.7 protective systems
Require in the time of the 7th day, antibacterial reduces by 99.9%, and Aspergillus niger and candida albicans are each reduces by 90%, and in 28 days continuous decrease, test by preservation challenge.
If the meansigma methods of any one micro organism quantity in three parallel tests of single bacterium inoculation, in the time of the 7th day, drop to below 100CFU/g (CFU/ml), in 28 days, be all down to 0CFU/g (CFU/ml), be considered as so antiseptic effect outstanding.
2.8 preservation challenge results
As seen from the above table, in emollient cream is tested the preservation challenge of staphylococcus aureus, escherichia coli, bacillus pyocyaneus, candida albicans and Aspergillus niger, in the time of the 7th day, staphylococcus aureus quantity bacterium is down to 0,, escherichia coli and bacillus pyocyaneus quantity reduced by 99.9%, the quantity of Candida albicans and Aspergillus niger has also reduced more than 90%, and along with time lengthening, the quantity of test bacterium continues to reduce, and reduces to 0 in the time of 28 days.To sum up, emollient cream is by the preservation challenge experiment to five kinds of bacterium.
It is anticorrosion that Application Example 2 is applied to cleansing milk
Cleansing milk formula
1. production technology:
By A heat phase to 80～85 ℃, stir.After A is uniformly dispersed mutually, add successively B phase, be uniformly dispersed.A, B two-phase stop heating after being uniformly dispersed, be cooled to 45 ℃ to add C phase, continue to be stirred to room temperature.
2. preservation challenge experiment
2.1 preservation challenge experimental techniques are the same
2.2 preservation challenge results
As seen from the above table, in emollient cream is tested the preservation challenge of staphylococcus aureus, escherichia coli, bacillus pyocyaneus, candida albicans and Aspergillus niger, in the time of the 7th day, the quantity bacterium of staphylococcus aureus, escherichia coli and bacillus pyocyaneus is down to 0; The quantity of Candida albicans and Aspergillus niger has also reduced more than 90%, and the quantity of five kinds of bacterium all reduces to 0 in the time of the 14th day.To sum up, emollient cream is by the preservation challenge experiment to five kinds of bacterium.
Should be understood that, wt% of the present invention all refers to quality percentage composition.
Above specific embodiments of the invention be have been described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and alternative also all among category of the present invention.Therefore, equalization conversion and the modification done without departing from the spirit and scope of the invention, all should contain within the scope of the invention.
Claims (10)
1. a natural plants compound recipe with antiseptic effect, is characterized in that, is by comprising Herba Pogostemonis, Herba Ocimi (Herba Ocimi Pilosi) and Herba Rosmarini Officinalis.
2. natural plants compound recipe according to claim 1, is characterized in that, the mass ratio of described Herba Pogostemonis is 0.33-99wt%, and the mass ratio of described Herba Ocimi (Herba Ocimi Pilosi) is 0.33-99wt%, described Herba Rosmarini Officinalis mass ratio be 0.33-99wt%.
3. natural plants compound recipe according to claim 1, is characterized in that, the mass ratio of described plant compound recipe is: the mass ratio of described Herba Pogostemonis is 10-80wt%, and the mass ratio of described Herba Ocimi (Herba Ocimi Pilosi) is 10-80wt%, described Herba Rosmarini Officinalis mass ratio be 10-80wt%.
4. according to arbitrary described natural plants compound recipe in claim 1-3, it is characterized in that: the extracting mode of described plant compound recipe is the mode of extracting again after mixing in the mode of variable concentrations proportioning or with Herba Pogostemonis, Herba Ocimi (Herba Ocimi Pilosi), Herba Rosmarini Officinalis crude drug after Herba Pogostemonis, Herba Ocimi (Herba Ocimi Pilosi), Herba Rosmarini Officinalis crude drug extract separately.
5. natural plants compound recipe according to claim 4, is characterized in that, the process conditions of described extracting mode are: adopt water and organic solvent as solvent, temperature is 50-99 ℃, and extraction time is 1-8h, and the mass ratio of described solvent and crude drug is 1:2-20.
6. natural plants compound recipe according to claim 5, it is characterized in that: described extraction conditions is: the ethanol that described solvent is 50-95wt%, described extraction temperature is 80-99 ℃, and described extraction time is 2-4h, and described solvent and the mass ratio of crude drug are 1:6-10.
7. according to arbitrary described natural plants compound recipe in claim 1-3, it is characterized in that, plant compound recipe after described extraction is using plant extract dicyandiamide solution as solvent, and described plant extract dicyandiamide solution is ethanol, propylene glycol, glycerol and/or 1,3 butylene glycol.
8. according to the natural plants compound recipe described in claim 1-7 any one, it is characterized in that: described plant compound recipe has the effect that suppresses staphylococcus aureus, escherichia coli, bacillus pyocyaneus, candida albicans, aspergillus niger.
9. according to the natural plants compound recipe described in claim 1-7 any one, it is characterized in that: described plant compound recipe can be applied to cream frost, emulsion, gel or water preparation.
10. natural plants compound recipe according to claim 9, is characterized in that: adding proportion when described plant compound recipe is applied to cream frost, emulsion, gel or water preparation is 1-3wt%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112057378A (en) * | 2020-08-25 | 2020-12-11 | 中北大学 | Botanical cosmetic preservative and preparation method thereof |
| CN113017237A (en) * | 2020-12-29 | 2021-06-25 | 吕满香 | Cosmetic brush containing natural active antibacterial ingredients and preparation method thereof |
| CN115475120A (en) * | 2022-08-18 | 2022-12-16 | 北京华霈家科技有限公司 | Composition for preparing plant extract with antiseptic and bacteriostatic effects, plant extract and application thereof |
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| CN1467274A (en) * | 2003-05-28 | 2004-01-14 | 郝建平 | Antibiotic plant essential oil, perfume and scented tea |
| CN103493846A (en) * | 2013-09-29 | 2014-01-08 | 上海莱博生物科技有限公司 | Preparation method for natural preservative micro-emulsion preparation |
| CN103520031A (en) * | 2013-09-29 | 2014-01-22 | 上海莱博生物科技有限公司 | Plant formula used as natural preservative |
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| CN1467274A (en) * | 2003-05-28 | 2004-01-14 | 郝建平 | Antibiotic plant essential oil, perfume and scented tea |
| CN103493846A (en) * | 2013-09-29 | 2014-01-08 | 上海莱博生物科技有限公司 | Preparation method for natural preservative micro-emulsion preparation |
| CN103520031A (en) * | 2013-09-29 | 2014-01-22 | 上海莱博生物科技有限公司 | Plant formula used as natural preservative |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112057378A (en) * | 2020-08-25 | 2020-12-11 | 中北大学 | Botanical cosmetic preservative and preparation method thereof |
| CN113017237A (en) * | 2020-12-29 | 2021-06-25 | 吕满香 | Cosmetic brush containing natural active antibacterial ingredients and preparation method thereof |
| CN115475120A (en) * | 2022-08-18 | 2022-12-16 | 北京华霈家科技有限公司 | Composition for preparing plant extract with antiseptic and bacteriostatic effects, plant extract and application thereof |
| CN115475120B (en) * | 2022-08-18 | 2024-03-26 | 北京华霈家科技有限公司 | Composition for preparing plant extract with antiseptic and antibacterial effects, plant extract and application thereof |
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