CN104522835A - Traditional Chinese medicinal herbal preservative, as well as preparation method and bacterium inhibition method thereof - Google Patents
Traditional Chinese medicinal herbal preservative, as well as preparation method and bacterium inhibition method thereof Download PDFInfo
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- CN104522835A CN104522835A CN201410819077.1A CN201410819077A CN104522835A CN 104522835 A CN104522835 A CN 104522835A CN 201410819077 A CN201410819077 A CN 201410819077A CN 104522835 A CN104522835 A CN 104522835A
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Abstract
The invention discloses a traditional Chinese medicinal herbal preservative, as well as a preparation method and a bacterium inhibition method thereof, and aims to solve the problem of injury on human bodies of existing chemical preservatives in food application. The preservative comprises extracts of the following raw materials: salviae miltiorrhizae, astragalus membranaceus, dandelion and houttuynia cordata. The preparation method and the bacterium inhibition method comprise preparation of traditional Chinese medicinal herbal extract, preparation of to-be-tested bacterium solution and bacterium inhibition tests. The preservative has the advantages of safety, harmlessness, wide antimicrobial spectrum and strong bacterium inhibition capability. Compared with a currently common chemical preservative, the preservative prepared by the method can be used for prolonging the preservative period of foods, improving the food safety and overcoming injury of chemical preservatives on human bodies; and the preparation method is simple and convenient, and is easy to implement.
Description
Technical field
The invention belongs to antistaling agent field, be specifically related to a kind of Chinese herbal medicine preservative.
Background technology
Food is in processing, storage, in process of consumption, be easy to be subject to the dip-dye of various microorganism and cause food apoilage, therefore anticorrisive agent is widely used in food processing preservation.Current domestic main use chemical preservative (as benzoic acid, sorbic acid and its esters etc.) carries out the anti-corrosive fresh-keeping of food, although serve good fresh-keeping effect, but chemical preservative has certain toxic and side effect (i.e. teratogenesis, carcinogenic, mutagenesis), potential harm is existed to health, therefore researches and develops wide spectrum, the historic mission that efficient, natural antiseptic agent that is that have no side effect becomes anticorrisive agent research worker.
Summary of the invention
The object of this invention is to provide a kind of Chinese herbal medicine preservative, to solve the problem to human injury that existing chemical preservative brings in food applications.
Another object of the present invention is to provide a kind of preparation method of Chinese herbal medicine preservative.
Another object of the present invention is to provide a kind of bacteriostasis method of Chinese herbal medicine preservative.
A kind of Chinese herbal medicine preservative, comprise the extract of following raw materials according: the red sage root, the Radix Astragali, dandelion, cordate houttuynia, its ratio is 1:1:1:1.
Preferred a kind of Chinese herbal medicine preservative, is made up of the extract of following raw materials according: the red sage root, the Radix Astragali, dandelion, and ratio is 1:1:1.
Preferred a kind of Chinese herbal medicine preservative, is made up of the extract of following raw materials according: the red sage root, the Radix Astragali, cordate houttuynia, and ratio is 1:1:1.
Preferred a kind of Chinese herbal medicine preservative, is made up of the extract of following raw materials according: the red sage root, dandelion, cordate houttuynia, and ratio is 1:1:1.
Preferred a kind of Chinese herbal medicine preservative, is made up of the extract of following raw materials according: the Radix Astragali, dandelion, cordate houttuynia, and ratio is 1:1:1.
A preparation method for Chinese herbal medicine preservative, comprises the following steps: cross 40 mesh sieves by rear for Chinese herbal medicine pulverizing, get powder 20-50g, adding 400-1000ml concentration is 50-60% ethanol, backflow 2-4h, distillation 1-2h, and filtration, collection filtrate is for subsequent use.Filtration makes filtrate quality concentration be 50mg/ml, and namely 1 ml extract is equivalent to 50 mg plant powders (dry weight), loads and has in the triangular flask of stopper, sealing, puts refrigerator (0-4 DEG C) refrigeration, for subsequent use.
A bacteriostasis method for Chinese herbal medicine preservative, comprises the following steps:
A. for the selection of examination bacterial classification
Have selected several bacterial classifications easily causing food apoilage: staphylococcus aureus (Staphylococcus aureus; GSICC 31902), Escherichia coli (Escherichia coli; GSICC 30507), bacillus subtilis (Bacillus cerrus, GSICC 32825); Above bacterial classification purchased from Research for Industrial Microbial Germ preservation center, Gansu Province, network address: www.gsmsc.cn.
B. for the preparation of examination bacterium liquid
For examination bacterial classification through inclined plane inoculating, cultivate 36-48 h at 35-37 DEG C of temperature after, will be connected in liquid bacterial culture medium for examination bacterium, at 35-37 DEG C of temperature, cultivate 36-48 h, make for examination bacterium liquid, refrigeration, for subsequent use.
C. antibacterial
Aseptically, pour the solid medium after sterilizing into sterile petri dish, horizontal rest is waited to coagulate, and gets 100-200 μ l bacterium drops on plate with sterilizing pipette, with spreading rod by even for the coating of bacterium liquid;
With the Oxford cup after aseptic nipper gripping sterilizing, put into the culture dish connecting bacterial classification, instillation 10-20 μ l extracts of Chinese herbal medicine, then put into constant incubator by culture dish, cultivate 36 h-48h, measure antibacterial circle diameter at 35-37 DEG C.
Preferably, the liquid bacterial culture medium in above-mentioned steps B consists of: peptone 10.0g, beef extract 3.0 g, Nacl 5.0g, distilled water 1000ml, pH value 7.2 ~ 7.4, sterilizing 30min at 121 DEG C of temperature.
Preferably, the solid medium in above-mentioned steps C consists of: peptone 10.0g, beef extract 3.0 g, Nacl 5.0g, agar 15.0g, distilled water 1000ml, pH value 7.2 ~ 7.4.Water being heated, adds beef extract, peptone, Nacl, adding agar when boiling to all dissolving, 121 DEG C of autoclaving 30min.
Natural antiseptic agent mainly refers to the anticorrisive agent deriving from microorganism, animal and plant, is that the anticorrisive agent of raw material gets more and more people's extensive concerning at present with plant.And terpene, alkaloid, flavonoids, steroidal, phenols etc. contained in Chinese herbal medicine all has antibacterial, antibacterial or insecticidal action.And, there is the synergistic function of antibiotic property between suitable medicinal herb components, and compatibility of drugs synergy is a kind of effective way increasing its antibacterial potency.The active component of combined Chinese herb and the mechanism of action are very complicated, the result of the composite effect necessarily mutual compound action of various composition, this compound action of Chinese herbal medicine, chemically on composition, the compound action between same medicinal herb components and between xenogenesis medicinal herb components may be there is, make its antibacterial effect be better than the bacteriostasis efficacy of simple.This method is started with from part integration of drinking and medicinal herbs Chinese herbal medicine, develops a kind of natural, safe food preservative.Chinese herbal medicine preservative provided by the invention adopts following several plant source Chinese medicine, is described below:
The red sage root is that dicotyledon Lamiaceae Salvia belongs to perennial vertical herbage plant, main containing fat-soluble Diterpenoids from bulbus and water miscible phenolic acid components, also containing flavonoids, and triterpenes, other compositions such as sterol.Have antibacterial, antibacterial, the effect such as relieve the pain.
Dandelion is composite family, belongs to herbaceos perennial, and dandelion herb is containing taraxasterol, choline, synanthrin, pectin etc.; Containing taraxol, taraxerol, the public English sterol in ψ-Portugal, taraxasterol, β-amyrin, stigmasterol, cupreol, choline, organic acid, fructose, sucrose, glucose, glycoside and resin, rubber etc. in root; Containing Arnica glycol, lutein and flavoxanthin in spending.Through clinical verification, dandelion has anti-inflammatory, sterilization, the effect such as antibacterial, anticorrosion,
Cordate houttuynia is the herbal medicine that Chinese Pharmacopoeia is included, and its extract all has inhibitory action to various microorganism (especially saccharomycete and mould), has obvious inhibitory action to staphylococcus aureus, micrococcus catarrhalis Escherichia coli, shigella dysenteriae, typhoid bacillus.
The Radix Astragali is the root of legume astragalus mongolicus or Astragalus membranacus, is one of conventional Chinese medicine.Main containing a few constituents such as saponin(e, flavones, polysaccharide and amino acid.These chemical compositions cause the microorganism of food spoilage to have stronger inhibit activities and antibacterial action to multiple.
Its main feature of antistaling agent prepared by the present invention is: safe, harmless, antimicrobial spectrum is wide, bacteriostasis is strong, can fully play anti-corrosive fresh-keeping effect in the application.The shelf-life of food is substantially increased while ensureing food security.
Detailed description of the invention
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Liquid bacterial culture medium in following embodiment consists of: peptone 10.0g, beef extract 3.0 g, Nacl 5.0g, distilled water 1000ml, pH value 7.2 ~ 7.4, sterilizing 30min at 121 DEG C of temperature.
Solid medium in following embodiment consists of: peptone 10.0g, beef extract 3.0 g, Nacl 5.0 g, agar 15.0 g, distilled water 1000ml, pH value 7.2 ~ 7.4.Method is heated by water, adds beef extract, peptone, Nacl, adds agar to all dissolving, 121 DEG C of autoclaving 30min when boiling.
The extract composition of embodiment 1. following raw materials according: the red sage root, the Radix Astragali, dandelion, ratio is 1:1:1.
A. each Chinese herbal medicine extracts respectively: take selected herbal powder 35g, adds the ethanol that 700ml concentration is 50%, backflow 3h, distillation 1.5h, and filtrate is for subsequent use; Filtration makes filtrate quality concentration be 50mg/ml, and namely 1 ml extract is equivalent to 50 mg plant powders (dry weight), loads and has in the triangular flask of stopper, sealing, puts refrigerator (4 DEG C) refrigeration, for subsequent use.
B. for the preparation of examination bacterium liquid: for examination bacterial classification staphylococcus aureus (Staphylococcus aureus, GSICC 31902), through inclined plane inoculating, cultivate (35 DEG C, 48 h) after, (35 DEG C will be cultivated for trying bacterium picking one ring in liquid bacterial culture medium, 48h), make for examination bacterium liquid, be placed in refrigerator cold-storage, for subsequent use;
C. bacteriostatic test:
C1. aseptically, pour the solid medium after sterilizing into sterile petri dish, horizontal rest is waited to coagulate, and gets 150 μ l bacterium drops on plate with sterilizing pipette, with spreading rod by even for the coating of bacterium liquid.
C2. use the Oxford cup after aseptic nipper gripping sterilizing, put into the culture dish connecting bacterial classification, instill the extracts of Chinese herbal medicine that 15 μ l mix in the cup of Oxford, then culture dish is put into constant incubator, at 36 DEG C, cultivate 42h, measure antibacterial circle diameter.
The extract composition of embodiment 2. following raw materials according: the red sage root, the Radix Astragali, cordate houttuynia, ratio is 1:1:1.
A. the extraction of each Chinese herbal medicine: take selected herbal powder 20g, adds the ethanol that 400ml concentration is 60%, backflow 2h, and distillation 1h, filtrate is for subsequent use.Filtration makes filtrate quality concentration be 50mg/ml, and namely 1 ml extract is equivalent to 50 mg plant powders (dry weight), loads and has in the triangular flask of stopper, sealing, puts refrigerator (4 DEG C) refrigeration, for subsequent use.
B. for the preparation of examination bacterium liquid: for examination bacterial classification Escherichia coli (Escherichia coli, GSICC 30507), through inclined plane inoculating, cultivate (36 DEG C, 42 h) after, (36 DEG C will be cultivated for trying bacterium picking one ring in liquid bacterial culture medium, 42h), make for examination bacterium liquid, be placed in refrigerator cold-storage, for subsequent use;
C. bacteriostatic test:
C1. dull and stereotyped coating inoculation
Aseptically, pour the solid medium after sterilizing into sterile petri dish, horizontal rest is waited to coagulate, and gets 100 μ l bacterium drops on plate with sterilizing pipette, with spreading rod by even for the coating of bacterium liquid.
C2. the mensuration of fungistatic effect
With the Oxford cup after aseptic nipper gripping sterilizing, put into the culture dish connecting bacterial classification, instill the extracts of Chinese herbal medicine that 10 μ l mix in the cup of Oxford, then culture dish is put into constant incubator, at 35 DEG C, cultivate 36h, measure antibacterial circle diameter.
The extract composition of embodiment 3. following raw materials according: the red sage root, dandelion, cordate houttuynia, ratio is 1:1:1.
A. the extraction of Chinese herbal medicine: take selected herbal powder 50g, adding 1000 ml concentration is the ethanol of 55%, backflow 4h, distillation 2h, and filtrate is for subsequent use; Filtration makes filtrate quality concentration be 50mg/ml, and namely 1 ml extract is equivalent to 50 mg plant powders (dry weight), loads and has in the triangular flask of stopper, sealing, puts refrigerator (0 DEG C) refrigeration, for subsequent use.
B. for the preparation of examination bacterium liquid: for examination species Bacillus subtilis (Bacillus cerrus, GSICC 32825), through inclined plane inoculating, cultivate (37 DEG C, 36 h) after, (37 DEG C will be cultivated for trying bacterium picking one ring in liquid bacterial culture medium, 36h), make for examination bacterium liquid, be placed in refrigerator cold-storage, for subsequent use;
C. bacteriostatic test:
C1. aseptically, pour the solid medium after sterilizing into sterile petri dish, horizontal rest is waited to coagulate, and gets 200 μ l bacterium drops on plate with sterilizing pipette, with spreading rod by even for the coating of bacterium liquid.
C2. use the Oxford cup after aseptic nipper gripping sterilizing, put into the culture dish connecting bacterial classification, instill the extracts of Chinese herbal medicine that 20 μ l mix in the cup of Oxford, then culture dish is put into constant incubator, at 35 DEG C, cultivate 36h, measure antibacterial circle diameter.
The extract composition of embodiment 4. following raw materials according: the Radix Astragali, dandelion, cordate houttuynia, ratio is 1:1:1, and the extraction of Chinese herbal medicine, the preparation supplying examination bacterium liquid and bacteriostatic test step are with embodiment 1.
The extract composition of embodiment 5. following raw materials according: the red sage root, the Radix Astragali, dandelion, cordate houttuynia, its ratio is 1:1:1:1, and the extraction of Chinese herbal medicine, the preparation supplying examination bacterium liquid and bacteriostatic test step are with embodiment 1.
One, for the screening process of examination Chinese herbal medicine:
Have the Chinese herbal medicine of bacteriostatic activity from following 10 kinds and screen: Radix Glycyrrhizae, cloves, the coptis, cordate houttuynia, Radix Isatidis, the red sage root, nutmeg, the root of Dahurain angelica, dandelion, the Radix Astragali.
A. the extraction of Chinese herbal medicine: take selected herbal powder 35g, adds the ethanol that 700ml concentration is 50%, backflow 3h, distillation 1.5h, and filtrate is for subsequent use;
B. for the preparation of examination bacterium liquid: for examination bacterial classification through inclined plane inoculating, cultivate (36 DEG C, 42 h) after, will cultivate (36 DEG C, 42h) in liquid bacterial culture medium for examination bacterium picking one ring, and make and supply to try bacterium liquid, be placed in refrigerator cold-storage, for subsequent use;
Two, bacteriostatic test:
C1. aseptically, pour the solid medium after sterilizing into sterile petri dish, horizontal rest is waited to coagulate, and gets 150 μ l bacterium drops on plate with sterilizing pipette, with spreading rod by even for the coating of bacterium liquid.
C2. the Oxford cup after aseptic nipper gripping sterilizing is used, put into the culture dish connecting bacterial classification, in each ware, put into three Oxford cups, wherein two instillation Oxford cup 15 μ l same extracts of Chinese herbal medicine, another one instills 15 μ l sterilized waters and contrasts, and each bacterial classification repeats to do three times.Then culture dish is put into constant incubator, 42h is cultivated at 36 DEG C, measure antibacterial circle diameter, carry out same test to 10 kinds of extracts of Chinese herbal medicine, the 4 kinds of extracts of Chinese herbal medicine filtering out good antimicrobial effect are the red sage root, the Radix Astragali, dandelion, cordate houttuynia, carry out composite to this, bacteriostatic test is carried out to 4 kinds of compositional liquors of gained, the antistaling agent filtering out fungistatic effect the best is the compositional liquor of embodiment 1-4, finds that fungistatic effect is it is preferred that the compositional liquor of embodiment 1, the results are shown in Table 1.
The different compositional liquor of table 1 is to the antibacterial circle diameter (mm) for examination bacterial classification
Claims (10)
1. a Chinese herbal medicine preservative, is characterized in that it comprises the extract of following raw materials according: the red sage root, the Radix Astragali, dandelion, cordate houttuynia, and its ratio is 1:1:1:1.
2.
a kind of Chinese herbal medicine preservative according to claim 1, is characterized in that: it is made up of the extract of following raw materials according: the red sage root, the Radix Astragali, dandelion, and ratio is 1:1:1.
3.
a kind of Chinese herbal medicine preservative according to claim 1, is characterized in that: it is made up of the extract of following raw materials according: the red sage root, the Radix Astragali, cordate houttuynia, and ratio is 1:1:1.
4. a kind of Chinese herbal medicine preservative according to claim 1, is characterized in that: it is made up of the extract of following raw materials according: the red sage root, dandelion, cordate houttuynia, ratio is 1:1:1.
5. a kind of Chinese herbal medicine preservative according to claim 1, is characterized in that: it is made up of the extract of following raw materials according: the Radix Astragali, dandelion, cordate houttuynia, ratio is 1:1:1.
6. the preparation method of a kind of Chinese herbal medicine preservative according to claim 1, is characterized in that it comprises the following steps:
Cross 40 mesh sieves by rear for Chinese herbal medicine pulverizing, get powder 20-50g, adding 400-1000ml concentration is 50-60% ethanol, backflow 2-4h, distillation 1-2h, and filtration, collection filtrate is for subsequent use.
7. the preparation method of a kind of Chinese herbal medicine preservative according to claim 6, is characterized in that: filter and make filtrate quality concentration be 50mg/ml, and sealing, refrigerates under setting to 0-4 DEG C of temperature.
8. the bacteriostasis method of a kind of Chinese herbal medicine preservative according to claim 1, is characterized in that it comprises the following steps:
A. for the selection of examination bacterial classification
Select the one in following bacterial classification: staphylococcus aureus (Staphylococcus aureus; GSICC 31902), Escherichia coli (Escherichia coli; GSICC 30507), bacillus subtilis (Bacillus cerrus, GSICC 32825);
B. for the preparation of examination bacterium liquid
For examination bacterial classification through inclined plane inoculating, cultivate 36-48 h at 35-37 DEG C of temperature after, will be connected in liquid bacterial culture medium for examination bacterium, at 35-37 DEG C of temperature, cultivate 36-48 h, make for examination bacterium liquid, refrigeration, for subsequent use;
C. antibacterial
Aseptically, pour the solid medium after sterilizing into sterile petri dish, horizontal rest is waited to coagulate, and gets 100-200 μ l bacterium drops on plate with sterilizing pipette, with spreading rod by even for the coating of bacterium liquid;
With the Oxford cup after aseptic nipper gripping sterilizing, put into the culture dish connecting bacterial classification, instillation 10-20 μ l extracts of Chinese herbal medicine, then put into constant incubator by culture dish, cultivate 36 h-48h, measure antibacterial circle diameter at 35-37 DEG C.
9. the bacteriostasis method of a kind of Chinese herbal medicine preservative according to claim 1, it is characterized in that: the liquid bacterial culture medium in described step B consists of: peptone 10.0g, beef extract 3.0 g, Nacl 5.0g, distilled water 1000ml, pH value 7.2 ~ 7.4, sterilizing 30min at 121 DEG C of temperature.
10. the bacteriostasis method of a kind of Chinese herbal medicine preservative according to claim 1, it is characterized in that: the solid medium in described step C consists of: peptone 10.0g, beef extract 3.0 g, Nacl 5.0 g, agar 15.0 g, distilled water 1000ml, pH value 7.2 ~ 7.4, sterilizing 30min at 121 DEG C of temperature.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839320A (en) * | 2015-05-15 | 2015-08-19 | 伍祖林 | Fruit preservative |
| CN105560148A (en) * | 2015-12-30 | 2016-05-11 | 庄奇陵 | Skin care product liquid with natural anti-corrosive function and extraction process thereof |
| CN106035609A (en) * | 2016-08-06 | 2016-10-26 | 尹浩 | Biogenic composite pork preservative |
| CN107927516A (en) * | 2017-11-07 | 2018-04-20 | 武晓丹 | A kind of food bacteriostatic agent and preparation method thereof |
| CN109090430A (en) * | 2018-09-20 | 2018-12-28 | 贵州省贵福菌业发展有限公司 | A kind of fresh fried flour mushroom is fresh-keeping to use lactic acid bacteria fresh-keeping liquid |
| CN109845802A (en) * | 2018-12-14 | 2019-06-07 | 马山县山山农业科技有限公司 | Beef bacteria reducing method |
-
2014
- 2014-12-25 CN CN201410819077.1A patent/CN104522835A/en active Pending
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104839320A (en) * | 2015-05-15 | 2015-08-19 | 伍祖林 | Fruit preservative |
| CN105560148A (en) * | 2015-12-30 | 2016-05-11 | 庄奇陵 | Skin care product liquid with natural anti-corrosive function and extraction process thereof |
| CN105560148B (en) * | 2015-12-30 | 2018-03-20 | 庄奇陵 | A kind of skin care item stoste and its extraction process with natural anticorrosion function |
| CN106035609A (en) * | 2016-08-06 | 2016-10-26 | 尹浩 | Biogenic composite pork preservative |
| CN107927516A (en) * | 2017-11-07 | 2018-04-20 | 武晓丹 | A kind of food bacteriostatic agent and preparation method thereof |
| CN109090430A (en) * | 2018-09-20 | 2018-12-28 | 贵州省贵福菌业发展有限公司 | A kind of fresh fried flour mushroom is fresh-keeping to use lactic acid bacteria fresh-keeping liquid |
| CN109845802A (en) * | 2018-12-14 | 2019-06-07 | 马山县山山农业科技有限公司 | Beef bacteria reducing method |
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Application publication date: 20150422 |