CN103830138A - Natural plant compound having preservative effect and daily health product - Google Patents
Natural plant compound having preservative effect and daily health product Download PDFInfo
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Abstract
The present invention relates to a natural plant extraction compound having preservative effect, comprising wrinkled giant hyssop, salvia officinalis and honeysuckle flower. The plant compound of the invention, having preservative effect, can substitute chemical preservatives for use, and reduces toxic and side effect of chemical preservatives.
Description
Technical field
The invention belongs to natural antiseptic agent field, relate to a kind of natural plants compound extract with antiseptic effect.
Background technology
Antibiotic antiseptic the earliest comes from nature, but the restriction of the problem such as action effect is poor, shortage of resources is replaced by chemosynthesis antibiotic antiseptic gradually owing to being subject to.Along with the mankind are healthy and environmental issue is familiar with improves constantly to self, people find that many chemosynthesis antibacterial have potential carcinogenic, teratogenesis and i.e. " three cause " effect of mutagenic action gradually, and the exploitation of new chemical synthetic antimicrobial antiseptic is more and more difficult, make natural antibiotic antiseptic be subject to once again people's favor.Except taking natural antimicrobial substance as raw material or model compound carry out the exploitation of chemical antibiotic antiseptic, also have many natural materials can directly be used as antibiotic antiseptic.Natural antibiotic antiseptic can be divided into plant-source antibacterial antiseptic, animal sources antibiotic antiseptic and microbial source antibiotic antiseptic by its source.If from chemical composition, natural antibiotic antiseptic can be divided into low molecule antibiotic antiseptic and high-molecular anti-bacteria antiseptic.The former is mainly alkaloid, organic acid, phenols and volatilization wet goods; Latter is mainly tannin, polysaccharide and protein.
According to incompletely statistics, the current plant that can be used as in the world antibiotic antiseptic has 2000 kinds of left and right at least.These plants all get a good chance of becoming the resource of antibiotic antiseptic exploitation.The feature of plant-source antibacterial antiseptic is that toxicity is low, source is abundant and cheap.
The chemical composition of plant source antiseptic agent is very complicated, this is main because biology forms and accumulated the unequal secondary metabolite of content in growth course, wherein a lot of chemical compositions all have important physiologically active, as glycoside, alkaloids, terpenoid and volatilization wet goods.The researchs such as Robert A show, the inhibitor that suppresses Aspergillus flavus is the cattle thing bases such as the phenylpropyl compounds such as flavone, flavonol, coumarin, chromone and flavonoid (polyphenol), terpenoid, caffeine, Fructus Piperis powder.Wu Chuanwan etc. describe the structure type of Natural Antibacterial Constituents from Plant Origin in detail, as terpenoid, alkaloids, flavonoid, glycoside, Saponin, coumarin and lignin, esters, aldehydes, phenols and alcohols, organic acid, quintessence oil class, Saponin, steroid class, stilbene class, Anthraquinones etc.The researchs such as Tao Yongxia show, tomato biological alkali has stronger inhibitory action to Putrefying bacteria common in food, yeast, and to the inhibitory action of mycete a little less than, flavone compound particularly isoflavone compounds also has very strong antibacterial action.The reports such as Yang Lin, the tannin in Pericarpium Granati and flavone compound have certain bacteriostasis.Yan Zankai etc. isolate hesperidin from Pericarpium Citri tangerinae, and it all has inhibitory action to microorganism common in food.The researchs such as Cheng Zhen show, the bacteriostatic active ingredients of seeds of Urtica cannabina is mainly phenolic compound, Coumarins and organic acid etc.The researchs such as Wang Hang show, effective antipathogenic composition of Radix Sophorae Flavescentis is the material that contains alkaloid, aldehydes matter and flavonoid, and effective antipathogenic composition of Radix Sanguisorbae is alkaloid and Flavonoid substances. and the main matter that plays bacteriostasis in Borneolum Syntheticum is quinones.Plants essential oil (as Adeps Bovis seu Bubali, Flos Caryophylli, Cortex Cinnamomi, Herba thymi vulgaris, Herba Menthae, Herba Rosmarini Officinalis, Herba Coriandri, Salvia japonica Thunb. etc.) and its one pack system (as eugenol, carvacrol, cinnamic acid, hexanal, thymol, carvone, cinnamic aldehyde, citral, geraniol etc.) food-borne bacterium is had to very strong inhibitory action: the contained complex chemical composition of plants essential oil, its chemical constitution can be divided into aliphatic, aromatic series and terpenoid three major types compound and its containing oxygen derivative as alcohol, aldehyde, ketone, acid, ether, ester, lactone etc., also has in addition nitrogenous and compound sulfur-bearing.Thin elegant woods etc. has been studied the antibacterial action of plants essential oil and the application in food industry thereof.
Herba Pogostemonis is Labiatae herbaceos perennial, and Herba Pogostemonis is containing volatile oil 0.28%, and main component is that estragole (methylchavicol) accounts for more than 80%.And contain anethole (anethole), anisaldehyde (anisaldehyde), limonene (limonene), methoxy cinnamaldehyde (P-methoxycinnamaldehyde), α-and-nopinene (pinene), 3-octanone (3-octanone), 1-OCOL (1-octen-3-ol), linalool (linalool), 1-Flos Caryophylli alkene (1-carypholene), beta-elemene (β-elemene), β-Humuleno (β-humu-lene), ylangene (α-farnesene (β-farnesene), γ-Cadinene (γ-cadinene), Rhizoma Acori Graminei alkene (calamenene), also contain cis-beta, gamma-olefine aldehydr (cis-beta, gamma-hexenal).Flavone compound: acacetin (acacetin), tilia element (tilianin), linarin (linarin), agastachoside (agastachoside), different agastachoside (isoagastachoside), Herba Pogostemonis essence (agastachin).Root is Crataegolic acid (crateglic acid) containing maslinic acid (mas-linic acid), oleanolic acid (oleanolic acid), 3-O-acetyl group oleanolic aldehyde (3-O-acetyl oleanolic aldehyde), acacetin, tilia element, agastachoside, daucosterol (daucosterol), cupreol (β-sitosterol), Dehydroagastol (dehydro-agastol), 1-methylene-6, 8-dihydroxy-5-methoxyl group-7-(1, 1-dimethyl methylol)-1, 2, 3, 4, 9, 10, 10, a-seven hydrogen-9-phenanthrenone [1-methylene-2, 4a-dimethyl-6, 8-dihydroxy-5-methoxy-7-(1, 1-dimethyl-hydroxy methyl)-1, 2, 3, 4, 10, 10a-heptahydro-9-phenanthroxe].
Experimental results show that, Herba Pogostemonis decoct (8%～15%) has inhibitory action to multiple pathogenic funguses such as trichophyton in vitro. and ether leachate, alcohol extract, water-leach liquor also can suppress multiple pathogenic fungus. and test shows, Herba Pogostemonis ether leachate, alcohol extract, water-leach liquor and decoct are respectively 3%, 5%, 10%, 15% to trichophyton interdigitale and the trichophytic Mlc of the sufficient sole of the foot, and the antibacterial ability of prompting leachate is strong than decoct.
Salvia japonica Thunb. also claims Salvia farinacea, common Salvia japonica Thunb. (common sage) or garden Salvia japonica Thunb. (garden sage).The perennial fragrant herbaceous plant of Labiatae (Lamiaceae), formal name used at school is Salviaofficinalis.Herb is containing cupreol (β-sitosterol), cupreol glycoside (β-sitosterol glucoside), ursolic acid (ursolic acid), oleanolic acid (oleanolic acid), 2α-Hydroxyursolic Acid (2 α-hydroxyursolic acid), termentic acid (tormentic acid), caffeic acid (caffeic acid), maslinic acid (maslinic acid), ethyl-β-D-galactopyranose glycoside (ethyl β-D-galactopyranoside).The dried abnormal smells from the patient of Salvia japonica Thunb. is dense, general normal while being used to boil soup class or the strong meat food of taste, add a little can relax taste, mix in salad and enjoy, effect flower that more can bring into play skin maintenance can be brought and make tea, giving out fragrance taste, oils and fats in the quiet body that can disappear, help circulation, there is effect antibacterial, antidiarrheal.
Flos Lonicerae is the general designation of Chinese crude drug and plant.Plant Flos Lonicerae has another name called Radix Ophiopogonis, is the perennial half evergreen winding bejuco of Caprifoliaceae." Flos Lonicerae " one come from Compendium of Material Medica, because Flos Lonicerae is just opened as white, after transfer yellow to, Flos Lonicerae hence obtains one's name.Medical material Flos Lonicerae is Caprifoliaceae woodbine Radix Ophiopogonis and congener dry flower or the first flower of opening of band.Flos Lonicerae is described as the good medicine of heat-clearing and toxic substances removing from ancient times.The sweet cold fragrance of its property, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil.Flos Lonicerae can dispelling wind-heat, also kind removing summer-heat blood poison, and for various febrile diseases, as fever of the body, dermexanthesis, send out the cards such as speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain, all effect is remarkable.Experiment in vitro shows, Flos Lonicerae all has certain inhibitory action to various pathogens as staphylococcus aureus, Hemolytic streptococcus, escherichia coli, dysentery bacterium, vibrio cholera, Bacillus typhi, Salmonella paratyphi etc., also effective to streptococcus pneumoniae, meningococcus, bacillus pyocyaneus, tubercule bacillus.Water logging agent is stronger than decoct effect, and leaf decoct is stronger than colored decoct effect.If share with Fructus Forsythiae, antibacterial range also can be complementary; Share with penicillin, can strengthen the antibacterial action of penicillin to resistant Staphylococcus aureus, this may be to have collaborative effect on anti-bacteria body internal protein is synthetic.
Summary of the invention
The object of the present invention is to provide one group of natural plants compound extract with anticorrosion activity, particularly relate to one group of natural plants compound recipe that can be used as cosmetics preservative with preservative effectiveness taking Herba Pogostemonis, Salvia japonica Thunb., Flos Lonicerae as raw material.
In order to realize foregoing invention object, the technical solution adopted in the present invention is: by existing extractive technique, adopt water and organic solvent extraction, extraction temperature is 50-99 DEG C, and extraction time is 1-8h, and extracting solvent ratios is 6-10 times of crude drug quality.Wherein, optimum extraction mode is 50%-95% ethanol extraction, and optimum extraction temperature is 80-99 DEG C, and the optimum extraction time is 2-4h, and optimum extraction solvent ratios is 6-10 times of crude drug quality.After mixing with variable concentrations proportioning and crude drug after plant compound extract extracts separately by various crude drugs, extract again two kinds of mode systems.The plant compound extract making comprises Herba Pogostemonis extracting solution 0.33-99wt%, Salvia japonica Thunb. extracting solution 0.33-99wt%, Flos Lonicerae extractive solution 0.33-99wt%, wherein optimum combination is Herba Pogostemonis extracting solution 10-90wt%, Salvia japonica Thunb. extracting solution 5-80wt%, Flos Lonicerae extractive solution 5-80wt%.
Natural plants compound extract of the present invention can also comprise Herba Rosmarini Officinalis, the plant such as Radix Salviae Miltiorrhizae, Herba Ocimi (Herba Ocimi Pilosi), Radix Scutellariae.
The invention has the advantages that: (1) provides one group of natural anticorrosion plant compound recipe, in order to instead of chemical antiseptic, this invention can solve " three cause " problem of chemical preservative, has effect safer, low stimulation; (2) provide one group of natural anticorrosion plant compound recipe, solve the problem that single plant prescription is difficult to possess natural plants antiseptic, by composite and synergism, this invention can suppress staphylococcus aureus, escherichia coli, bacillus pyocyaneus, candida albicans and aspergillus niger simultaneously, add to by a certain percentage in daily chemical products, well instead of chemical antiseptic, can test by preservation challenge.
Detailed description of the invention
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.
Embodiment mono-: the preparation of natural anticorrosion plant compound recipe 1
Get Herba Pogostemonis 10g, Salvia japonica Thunb. 40g, Flos Lonicerae 50g, after medicine is pulverized, packs in the round-bottomed flask of 2L, adding 0.8L volume fraction is 50% alcoholic solution, put into electric jacket 95 DEG C of reflux, extract, 3 hours, extracting solution is concentrated to 50g through Rotary Evaporators, adds propylene glycol 50g, solvent components does not filter, and makes plant compound recipe 1.
Embodiment bis-: the preparation of natural anticorrosion plant compound recipe 2
Get Herba Pogostemonis 30g, Salvia japonica Thunb. 40g, Flos Lonicerae 40g, after pulverizing medicine, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 1.2L is 75%, puts into electric jacket 80 DEG C of reflux, extract, 3 hours, and extracting solution is concentrated to 40g through Rotary Evaporators, add 1,3-butanediol 60g, solvent components does not filter, and makes plant compound recipe 2.
Embodiment tri-: the preparation of natural anticorrosion plant compound recipe 3
Get Herba Pogostemonis 90g, Salvia japonica Thunb. 5g, Flos Lonicerae 5g, after medicine is pulverized, packs in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 0.8L is 95%, put into electric jacket 95 DEG C of reflux, extract, 3 hours, extracting solution is concentrated to 80g through Rotary Evaporators, adds glycerol 20g, solvent components does not filter, and makes plant compound recipe 3.
Embodiment tetra-: the preparation of natural anticorrosion plant compound recipe 4
Get Herba Pogostemonis 10g, Salvia japonica Thunb. 80g, Flos Lonicerae 10g, after medicine is pulverized, packs in the round-bottomed flask of 3L, the alcoholic solution that the volume fraction that adds 2L is 75%, put into electric jacket 80 DEG C of reflux, extract, 8 hours, extracting solution is concentrated to 60g through Rotary Evaporators, adds ethanol 40g, solvent components does not filter, and makes plant compound recipe 4.
Embodiment five: the preparation of natural anticorrosion plant compound recipe 5
Get Herba Pogostemonis 10g, Salvia japonica Thunb. 10g, Flos Lonicerae 80g, after medicine is pulverized, packs in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 1.5L is 50%, put into electric jacket 90 DEG C of reflux, extract, 3 hours, extracting solution is concentrated to 50g through Rotary Evaporators, adds glycerol 50g, solvent components does not filter, and makes plant compound recipe 5.
Embodiment six: the preparation of natural anticorrosion plant compound recipe 6
Get Herba Pogostemonis 100g, after medicine is pulverized, pack in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 0.8L is 95%, puts into electric jacket 75 DEG C of reflux, extract, 3 hours, and extracting solution is concentrated to 50g through Rotary Evaporators.Get Salvia japonica Thunb. 100g, after medicine is pulverized, pack in the round-bottomed flask of 2L, add 80% the alcoholic solution of 1.0L, put into electric jacket 95 DEG C of reflux, extract, 5 hours, extracting solution is concentrated to 50g through Rotary Evaporators.Extracting honeysuckle 100g, after medicine is pulverized, packs in the round-bottomed flask of 2L, adds 50% the alcoholic solution of 1.2L, puts into electric jacket 80 DEG C of reflux, extract, 8 hours, and extracting solution is concentrated to 50g through Rotary Evaporators.Three kinds of extracting solution are pressed to 1:1:1 and mix, then add the propylene glycol of same volume, solvent components does not filter, and makes plant compound recipe 6.
Embodiment seven: the preparation of natural anticorrosion plant compound recipe 7
Get Herba Pogostemonis 35g, Salvia japonica Thunb. 35g, Flos Lonicerae 30g, after medicine is pulverized, packs in the round-bottomed flask of 2L, the alcoholic solution that the volume fraction that adds 0.8L is 75%, put into electric jacket 85 DEG C of reflux, extract, 3 hours, extracting solution is concentrated to 50g through Rotary Evaporators, adds glycerol 50g, solvent components does not filter, and makes plant compound recipe 7.
Effect embodiment: MIC pH-value determination pH
1 test strain
Staphylococcus aureus (ATCC8739), escherichia coli (ATCC6538), bacillus pyocyaneus (ATCC9027), candida albicans (ATCC10231), Aspergillus niger (ATCC16404).
2 culture medium
(1) TSB solid medium: peptone 10g/L, NaCl10g/L, K2HPO42.5g/L, yeast extract 3g/L, agar 15g/L, distilled water dissolves, and regulating pH is 7.2 ± 0.2,115 DEG C of autoclaving 20min; TSB fluid medium: peptone 10g/L, NaCl10g/L, K2HPO42.5g/L, yeast extract 3g/L, distilled water dissolves, and regulating pH is 7.2 ± 0.2,115 DEG C of autoclaving 20min;
(2) SDB solid medium: peptone 10g/L, glucose 20g/L, K2HPO43g/L, yeast extract 5g/L, agar 15g/L, distilled water dissolves, 115 DEG C of autoclaving 20min; SDB fluid medium: peptone 10g/L, glucose 20g/L, K2HPO43g/L, yeast extract 5g/L, distilled water dissolves, 115 DEG C of autoclaving 20min.
3. collecting cells and inoculation
(1) get the staphylococcus aureus of glycerol preservation, escherichia coli, bacillus pyocyaneus 100 μ L coat on TSB solid medium, cultivate 2d for 37 DEG C, resuspended with normal saline, obtain bacteria suspension, and with plating method counting, test bacteria concentration are adjusted into 10
5～10
6cFU/mL.
(2) candida albicans and Aspergillus niger 100 μ L coat on SDB solid medium, cultivate 2d for 30 DEG C, resuspended with normal saline, obtain bacteria suspension, and with plating method counting, test bacteria concentration are adjusted into 10
5～10
6.
The mensuration of 4 minimum inhibitory concentrations (MIC value)
Testing sample plant compound recipe 1-7 is become to experimental concentration with Herba Pogostemonis extracting solution, Salvia japonica Thunb. extracting solution, Flos Lonicerae extractive solution with normal saline dilution.With 2 × TSB or 2 × SDB fluid medium dilution bacteria suspension, making its final bacteria concentration is l0
5cFU/mL.In micropore, add bacterium liquid 100 μ L and medicinal liquid 100 μ L, establish the normal growth contrast that does not add the negative control of bacterium and do not add medicinal liquid simultaneously, every kind of medicine do 3 parallel, average.Put 37 DEG C or 30 DEG C of wet boxes and hatch, observed result after 48h, adopts direct method reading out data.The prerequisite of result judgement is that growth control is good, and blank asepsis growth is clear, and with the rising of drug level gradient, the growth of bacterium is suppressed in other hole.
5 results
Application Example one: be applied to moisturizing water preparation anticorrosion
Table 1 moisture retention water agent prescription
1. preparation technology:
First hyaluronate sodium is dispersed in glycerol, then adds dissipation of heat (85 DEG C), after being uniformly dispersed, add other components of A phase to stir; D is dissolved each other mutually in advance; A is cooled to 45 degree mutually, adds B, C, D phase; After stirring, discharging .PH is controlled at 5.5-7.0.
2. preservation challenge experiment:
2.1 test strain
Staphylococcus aureus (ATCC8739), escherichia coli (ATCC6538), bacillus pyocyaneus (ATCC9027), candida albicans (ATCC10231), Aspergillus niger (ATCC16404).
2.2 culture medium
TSB solid medium: peptone 10g/L, NaCl10g/L, K
2hPO
42.5g/L, yeast extract 3g/L, agar 15g/L, distilled water dissolves, and regulating pH is 7.2 ± 0.2,115 DEG C of autoclaving 20min.SDB solid medium: peptone 10g/L, glucose 20g/L, K
2hPO
43g/L, yeast extract 5g/L, agar 15g/L, distilled water dissolves, 115 DEG C of autoclaving 20min.Lecithin tween 80 nutrient agar culture medium, is purchased from traditional Chinese medicines chemical reagent.Testing sample need be beforehand with microbial count and detect, and preservation challenge must be aseptic with sample.
2.3 preparations for sample product
Fluid sample: water miscible fluid sample, can measure 10mL and be added in 90mL sterile saline, as sample is less than 10mL, still undertaken by 10 times of dilution methods.As being added to 45mL sterile saline for 5mL, after mixing, making volume ratio is 1:10 inspection liquid.
Oil-based liquid sample, sample thief 10mL, first adding 5mL sterilized liquid paraffin mixes, add again the Tween 80 of 10mL sterilizing, in 40 DEG C～44 DEG C water-baths, vibration mixes 10min, add normal saline 75mL(pre-temperature in 40 DEG C～44 DEG C water-baths of sterilizing), emulsifying in 40 DEG C～44 DEG C water-baths, makes the suspension that volume ratio is 1:10.
Cream, frost, Emulsion semi-solid sample: hydrophilic sample: take 10g, be added in the triangular flask that bead and 90mL sterile saline are housed, fully vibration mixes, and leaves standstill 15min.Inspection liquid with its supernatant as 1:10.Hydrophobicity sample: take 10g, be put in the mortar of sterilizing, add 10mL sterilized liquid paraffin, grind to form thickly, then add 10mL sterilizing Tween 80, grind to be dissolved after, add 70mL sterile saline, in 40 DEG C～44 DEG C water-baths, fully mix, making volume ratio is 1:10 inspection liquid.
Solid sample: take 10g, be added in 90mL sterile saline, fully vibration mixes, and makes it disperse suspendible, after leaving standstill, gets the inspection liquid of supernatant as 1:10.
If any homogenizer, above-mentioned water solublity cream, frost, powder etc., can claim 10g sample to add 90mL sterile saline, homogenizing 1min～2min; Hydrophobicity cream, frost and eyebrow pencil, lipstick etc., claim 10g sample, adds 10mL sterilized liquid paraffin, 10mL Tween 80,70mL sterile saline, homogenizing 3min～5min.
2.4 operating procedure
According to concentration of preservatives, 1:10 can be examined to liquid and be diluted to again 1:100,1:1000 ... Deng, eliminate the effect of antiseptic.Draw inspection liquid 2mL, be injected into respectively in two sterilizing plates every ware 1mL.
Be poured in plate melting and being chilled to the lecithin tween 80 nutrient agar culture medium of 45～50 DEG C, the about 15mL of every ware, rotates plate immediately, sample is fully mixed homogeneously with culture medium, after agar solidifies, upset plate, puts cultivation 48h ± 2h in 36 DEG C ± 1 DEG C incubator.Separately get an empty plate of sterilizing that does not add sample, add about 15mL lecithin tween 80 nutrient agar culture medium, after agar solidifies, upset plate, puts in 36 DEG C ± 1 DEG C incubator and cultivates 48h ± 2h, is blank.
Mode and the quantity of 2.5 preservation challenge inoculations
Adopt single bacterium inoculation, get 5ml testing sample in test tube, accessing respectively staphylococcus aureus, escherichia coli and bacillus pyocyaneus is 1 × 10 to inoculating quantity
6cFU/g or 1 × 10
6cFU/ml; Candida albicans and Aspergillus niger inoculation quantity are 1 × 10
4cFU/g or 1 × 10
4cFU/ml.Every kind of bacterium each three parallel.
2.6 preservation challenge separation detection
Postvaccinal sample is in special time separation detection: 0 hour (after inoculation, stirring and evenly mixing, at once sampling), 2 days, 7 days, 14 days and 28 days.Operating procedure: first prepare TSB and SDB culture medium flat plate; After sampling, according to experiment needs, with 10 times, 100 times of normal saline dilutions and 1000 times etc., get 100 μ L and coat TSB and SDB culture medium flat plate, 28 DEG C ± 1 DEG C or 36 DEG C ± 1 DEG C cultivation; Colony counting.
The efficiency evaluation standard of 2.7 protective systems
Require in the time of the 7th day, antibacterial reduces by 99.9%, and Aspergillus niger and candida albicans are each reduces by 90%, and in 28 days continuous decrease, test by preservation challenge.
If the meansigma methods of any one micro organism quantity in three parallel tests of single bacterium inoculation, in the time of the 7th day, drop to below 100CFU/g (CFU/ml), in 28 days, be all down to 0CFU/g (CFU/ml), be considered as so antiseptic effect outstanding.
2.8 preservation challenge results
As can be seen from the above table, in the time that preservation challenge is tested the 7th day, staphylococcus aureus, escherichia coli and bacillus pyocyaneus quantity have reduced by 99.9%, and Candida albicans and Aspergillus niger quantity have reduced more than 90%; Along with the testing time extends, the quantity of five kinds of bacterium continues to reduce, and all reduces to 0 in the time of the 28th day.To sum up, emollient cream is tested by preservation challenge, and antiseptic effect is outstanding.
Application Example two: be applied to the anticorrosion of emollient cream
Table 2 emollient cream formula
1. preparation technology: first hyaluronate sodium is dispersed in glycerol, then adds dissipation of heat (85 DEG C), add other components of B phase to stir after being uniformly dispersed; Mutually each A component mix homogeneously, in 85 DEG C, is fully uniformly mixed; C is fully dissolved and stirred mutually; By mutually each D component mix and blend, be suitably heated to dissolving and be transparence; Before homogenizing, C, D are added to A and stir mutually, then add together B phase, homogenizing stirs 4 minutes; The complete stirring froth breaking of homogenizing, and cooling; Add the mutually each component of E in 45 DEG C, discharging after stirring, PH is controlled at 5.5-7.0.
2. preservation challenge experiment
2.1 preservation challenge experimental techniques are the same
2.2 preservation challenge results
As seen from the above table, in the time of the 7th day, staphylococcus aureus, escherichia coli and bacillus pyocyaneus quantity have reduced by 99.9%, the quantity of candida albicans and Aspergillus niger has reduced by 99%, and along with time lengthening, the quantity of test bacterium continues to reduce, and is reduced to 0 in the time of the 28th day, be considered as this emollient cream by the preservation challenge experiment to staphylococcus aureus, escherichia coli, bacillus pyocyaneus, candida albicans and Aspergillus niger, and antiseptic effect is outstanding.
Should be understood that, wt% of the present invention all refers to quality percentage composition.
Above specific embodiments of the invention be have been described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and alternative also all among category of the present invention.Therefore, equalization conversion and the amendment done without departing from the spirit and scope of the invention, all should contain within the scope of the invention.
Claims (10)
1. a natural plants compound recipe with antiseptic effect, composition comprises Herba Pogostemonis, Salvia japonica Thunb. and Flos Lonicerae.
2. natural plants compound recipe according to claim 1, is characterized in that, the mass ratio of described each composition is: Herba Pogostemonis 0.33-99wt%, Salvia japonica Thunb. 0.33-99wt%, Flos Lonicerae 0.33-99wt%.
3. natural plants compound recipe according to claim 2, is characterized in that, the mass ratio of described each composition is: Herba Pogostemonis extract 10-90wt%, sage extract 5-80wt%, Flos Lonicerae extract 5-80wt%.
4. according to arbitrary described natural plants compound recipe in claim 1-3, it is characterized in that: the extracting mode of described plant compound recipe is to extract after mixing with variable concentrations proportioning or crude drug after Herba Pogostemonis, Salvia japonica Thunb., Flos Lonicerae crude drug extract separately again.
5. natural plants compound recipe according to claim 4, is characterized in that: this plant compound recipe is with water and organic solvent extraction, and extraction temperature is 50-99 DEG C, and extraction time is 1-8h, and extracting quantity of solvent is 2-20 times of crude drug quality.
6. natural plants compound recipe according to claim 5, it is characterized in that: the described ethanol that is 50-95wt% for the water that extracts and solvent, described extraction temperature is 80-99 DEG C, and described extraction time is 2-4h, and described extraction solvent ratios is 6-10 times of crude drug quality.
7. according to arbitrary described natural plants compound recipe in claim 1-3, it is characterized in that, the plant compound recipe after described extraction is using conventional plant extract dicyandiamide solution as solvent.
8. natural plants compound recipe according to claim 7, is characterized in that, described conventional plant extract dicyandiamide solution is ethanol, propylene glycol, the mixing of one or more in glycerol, 1,3 butylene glycol solution.
9. according to arbitrary described natural plants compound recipe in claim 1-8, it is characterized in that: described plant compound recipe has the effect that suppresses staphylococcus aureus, escherichia coli, bacillus pyocyaneus, candida albicans, aspergillus niger.
10. a daily chemical products, comprises the natural plants compound recipe as described in claim 1-8 any one, and described daily chemical products is cream frost, emulsion, gel, water preparation, and the adding proportion of described natural plants compound recipe is 2-5wt%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076246A (en) * | 2015-08-28 | 2015-11-25 | 无限极（中国）有限公司 | Antibacterial traditional Chinese medicine composition, preparing method of antibacterial traditional Chinese medicine composition and application of antibacterial traditional Chinese medicine composition |
| CN105411935A (en) * | 2015-12-16 | 2016-03-23 | 广州市科能化妆品科研有限公司 | Preparation method of honeysuckle extract and application of honeysuckle extract to preservative-free cosmetics |
| WO2018163192A1 (en) * | 2017-03-06 | 2018-09-13 | Jagannathan V T | Herbal extract for promoting and managing benign prostatic hypertrophy (bph) and related ageing symptoms using extract from ageratum spp. |
| CN116869888A (en) * | 2023-08-03 | 2023-10-13 | 清远市望莎生物科技有限公司 | Natural plant preservative composition with antibacterial effect and preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520030A (en) * | 2013-09-29 | 2014-01-22 | 上海莱博生物科技有限公司 | Preparation method of Chinese herbal compound with natural anti-corrosion effect |
-
2014
- 2014-02-17 CN CN201410053299.7A patent/CN103830138A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520030A (en) * | 2013-09-29 | 2014-01-22 | 上海莱博生物科技有限公司 | Preparation method of Chinese herbal compound with natural anti-corrosion effect |
Non-Patent Citations (4)
| Title |
|---|
| 吴周和等: "藿香中天然防腐剂的提取方法及其抑菌作用研究", 《中国调味品》 * |
| 吴周和等: "藿香中天然防腐剂的提取方法及其抑菌作用研究", 《中国调味品》, no. 8, 31 August 2004 (2004-08-31) * |
| 律之: "神奇鼠尾草", 《清洗世界》 * |
| 律之: "神奇鼠尾草", 《清洗世界》, no. 20038, 31 December 2003 (2003-12-31), pages 44 - 45 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076246A (en) * | 2015-08-28 | 2015-11-25 | 无限极（中国）有限公司 | Antibacterial traditional Chinese medicine composition, preparing method of antibacterial traditional Chinese medicine composition and application of antibacterial traditional Chinese medicine composition |
| JP2018520985A (en) * | 2015-08-28 | 2018-08-02 | ▲無▼限▲極▼（中国）有限公司Ｉｎｆｉｎｉｔｕｓ （Ｃｈｉｎａ） Ｃｏｍｐａｎｙ Ｌｔｄ | Antibacterial Chinese medicine composition, method for producing the same |
| EP3281527A4 (en) * | 2015-08-28 | 2018-10-03 | Infinitus (China) Company Ltd. | Antimicrobial traditional chinese medicine composition, and preparation method and use thereof |
| US10321689B2 (en) | 2015-08-28 | 2019-06-18 | Infinitus (China) Company Ltd. | Antimicrobial traditional Chinese medicine composition, and preparation method and use thereof |
| CN105411935A (en) * | 2015-12-16 | 2016-03-23 | 广州市科能化妆品科研有限公司 | Preparation method of honeysuckle extract and application of honeysuckle extract to preservative-free cosmetics |
| WO2018163192A1 (en) * | 2017-03-06 | 2018-09-13 | Jagannathan V T | Herbal extract for promoting and managing benign prostatic hypertrophy (bph) and related ageing symptoms using extract from ageratum spp. |
| CN116869888A (en) * | 2023-08-03 | 2023-10-13 | 清远市望莎生物科技有限公司 | Natural plant preservative composition with antibacterial effect and preparation and application thereof |
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