CN105411935A - Preparation method of honeysuckle extract and application of honeysuckle extract to preservative-free cosmetics - Google Patents
Preparation method of honeysuckle extract and application of honeysuckle extract to preservative-free cosmetics Download PDFInfo
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- CN105411935A CN105411935A CN201510942149.6A CN201510942149A CN105411935A CN 105411935 A CN105411935 A CN 105411935A CN 201510942149 A CN201510942149 A CN 201510942149A CN 105411935 A CN105411935 A CN 105411935A
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- flos lonicerae
- cosmetics
- extract
- preparation
- preservative
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/81—Preparation or application process involves irradiation
Abstract
The invention discloses a preparation method of a honeysuckle extract meeting cosmetic formula requirements and an application of the honeysuckle extract to preservative-free cosmetics. According to the preparation method, a humectant propylene glycol for cosmetics is innovatively used as an extracting solvent, the honeysuckle extract is obtained under specific preparation conditions, and hazards of an organic solvent used in conventional extraction are avoided. The extract has preservative and antibacterial functions, and 0.05%-30% of the extract is added to cosmetics and can be taken as a plant-sourced preservative component for preparation of the preservative-free cosmetics. The used solvent is a common humectant for the cosmetics, is safe and non-toxic and has a skin care effect.
Description
Technical field
The present invention relates to plant extract field and cosmetic field, be specially a kind of extracting method being applicable to the Flos Lonicerae extract of cosmetics, and extract is applied in cosmetics as antiseptic and inhibiting bacteria function composition.
Background technology
Flos Lonicerae has another name called Flos Lonicerae, Flos Lonicerae, two precious flowers, is traditional parts of generic medicinal plants.Main containing volatile oil, flavonoid, organic acid, triterpenes and inorganic elements.Modern pharmacology research finds that Flos Lonicerae has three large effects: resisting pathogenic microbes, enhancing immunologic function and antiinflammatory refrigeration function.Flos Lonicerae extract adds in cosmetics and has antibacterial Shu Min, slow down aging, effect such as injury of preventing ultraviolet from producing.
The achievement in research of Microwave Assisted Extraction Technique in plant extract has multinomial license.As existing applied microwave extracts the report of the rose anthocyanidin in Flos Rosae Rugosae and the gadoloside in Radix Rhodiolae.Result shows, multistage microwave amplifier products obtained therefrom has that impurity content is low, effective ingredient is high, and the advantage that extraction time is short.Microwave extracting have quick, energy-conservation, save solvent, pollute the advantages such as little, selectivity heating can be carried out to different compound, be conducive to extracting heat-labile material, avoid the substance decomposition that long-time high temperature causes.The main technologic parameters of microwave extracting comprises extractant, power and time etc., and wherein solvent is most important.
The Flos Lonicerae reported is extracted and is mostly using ethanol as solvent, if ethanol and microwave are in conjunction with chlorogenic acid extracting (CN200510059798.8), and microwave, enzymolysis and alcohol reflux (CN201110284568.7).But in cosmetics preparation process, add ethanol, because its volatility can make finished product with alcohol smell more or less, and most of user is not liked, in addition, the raw material added in cosmetic formulations with high-load ethanol likely produces some technical problems, such as thinning or produce floccule etc.Therefore the extracting solution prepared by the alcohol extracting method of ethanol is adopted to be not be suitable for be added in cosmetics completely.
Current Cosmetic Market faces problems in the selection of antiseptic, antiseptic adds excessive or selects improper, the zest of product may be caused even to cause allergy, therefore namely will ensure Product Safety, meet market trend again, just can satisfy the demands of consumers.
Summary of the invention
The technical problem to be solved in the present invention
There is provided a kind of be applicable to the Flos Lonicerae extracting method of cosmetics and extract is joined in cosmetics as antiseptic and inhibiting bacteria function agent, reduce the addition of traditional preservatives, thus reduce the zest of cosmetics and irritated rate, improve Product Safety.
For solving the problem, the present invention uses Moisturizing Agent Used in Cosmetic propylene glycol as Extraction solvent innovatively, and takes the method for microwave extraction to obtain Flos Lonicerae extract, simultaneously using the content of chlorogenic acid as the investigation target of extraction process quality.Propylene glycol, because it is with-OH polar group, can make most Organic substance be solubilized into water, and character is more similar with ethanol, but does not have volatility, can not bring penetrating odor.The custom that the present invention manufactures according to cosmetics, the extractive technique of maturation improves, to adapt to the demand of adding in cosmetics.
The object of the present invention is to provide a kind of Flos Lonicerae extract, use Moisturizing Agent Used in Cosmetic propylene glycol as Extraction solvent, avoid the harm that traditional extraction organic solvent used may bring, guarantee safety and the effectiveness of extract.
The Flos Lonicerae extract obtained by said method is safe and effective, joins in cosmetics as vegetable active additive, and addition is the 0.05%-30% of cosmetics.
The present invention extracts Flos Lonicerae effective bacteriostatic ingredients as solvent by using polyhydric alcohol used for cosmetic, and adds extract to cosmetics.Adding alternative or reducing the use of chemical preservative of Flos Lonicerae extract, improves the safety of cosmetics.
For the scheme of dealing with problems
The invention provides a kind of Flos Lonicerae extracting method, its feature is to comprise the following steps: the preparation method of Flos Lonicerae extract, comprise after Flos Lonicerae is ground, get and put into steeping tank in right amount and add a certain proportion of aqueous solution of propylene glycol, the solid-liquid ratio of described Flos Lonicerae and aqueous solution of propylene glycol is 1:5-1:60, in aqueous solution of propylene glycol, water and propylene glycol mass ratio are 2 ~ 1:1 ~ 4, stir and soak 8-24 hour, then treatment fluid is proceeded in extractor and carry out microwave extracting, extraction time is 5-40 minute, Extracting temperature is 25-125 DEG C, microwave power is 300-1500W, extract is filtered and obtains Flos Lonicerae extract.
Invention further provides the purposes of Flos Lonicerae extract in cosmetics.Wherein, the addition of Flos Lonicerae extract in cosmetics is 0.1%-30%.
Flos Lonicerae extract of the present invention is used for without in anticorrosion cosmetic, describedly comprises cold cream frost, emulsion, water preparation, essence, mask without anticorrosion cosmetic, and skin-clean cream is white, one in emulsion.
Brief description of drawings:
Fig. 1 is the chromatogram of chlorogenic acid standard substance.
Fig. 2 is the chromatogram extracting the Flos Lonicerae extractive solution obtained by the method for the application
Beneficial effect of the present invention
Compared with prior art, the present invention makes to apply some make up conventional moisturizing ingredient as Extraction solvent in the preparation process of Flos Lonicerae extract, and make extract safe and effective, skin affinity is better.Flos Lonicerae extract is used for the research and development of products without anticorrosion cosmetic by simultaneously the present invention innovatively, and that is prepared by this extract avoids without anticorrosion cosmetic the untoward reaction that chemical preservative may bring, and product gentleness does not stimulate.
Detailed description of the invention
Flos Lonicerae extract is prepared by the preparation method comprised the following steps: comprise after being ground by Flos Lonicerae, get and put into steeping tank in right amount and add a certain proportion of aqueous solution of propylene glycol, the solid-liquid ratio of described Flos Lonicerae and aqueous solution of propylene glycol is 1:5-1:60, in aqueous solution of propylene glycol, water and propylene glycol mass ratio are 2 ~ 1:1 ~ 4, stir and soak 8-24 hour, then treatment fluid is proceeded in extractor and carry out microwave extracting, extraction time is 5-40 minute, Extracting temperature is 25-125 DEG C, microwave power is 300-1500W, is filtered by extract and obtains Flos Lonicerae extract.
Preferred preparation condition is the solid-liquid ratio of Flos Lonicerae and aqueous solution of propylene glycol is 1:20, in aqueous solution of propylene glycol, water and propylene glycol mass ratio are 1:4, stir immersion 12 hours, then treatment fluid is proceeded in extractor and carry out microwave extracting, extraction time is 30 minutes, Extracting temperature is 25 DEG C, and microwave power is 900W.Flos Lonicerae extract (chlorogenic acid) content prepared by this optimum condition is the highest.
Embodiment
1. the selection of preparation condition
The content of the Flos Lonicerae extract (chlorogenic acid) that the different preparation condition of table 1. obtains
As shown in Table 1, the Flos Lonicerae extract (chlorogenic acid) prepared according to the different preparation condition of the present invention all can obtain higher chlorogenic acid content, and wherein Flos Lonicerae extract (chlorogenic acid) content prepared of the preparation condition shown in embodiment 15 is the highest, for 0.92mg/g, it is the preferred preparation condition of the present invention.And Flos Lonicerae extract (chlorogenic acid) content not adopting preparation condition of the present invention to obtain is low.
2. high performance liquid chromatography qualitative analysis
Following spectrogram shows that detecting thing occurs maximum absorption band at 3.827min and 3.832min place, and can conclude that two kinds of materials are same compound, preliminary judgement extract contains chlorogenic acid and impurity content is less.
Fig. 1 is the chromatogram of chlorogenic acid standard substance.
Fig. 2 is the chromatogram extracting the Flos Lonicerae extractive solution obtained by method of the present invention
3. Flos Lonicerae extractive solution inhibition evaluations
3.1 adopt disc diffusion method to measure antibacterial circle diameter
3.1.1 strain subject: purchased from Guangdong Microbes Inst
A. antibacterial
Staphylococcus aureus (Staphylococcusaureus): ATCC6538
Escherichia coli (Escherchiacoli): ATCC8739
Pseudomonas aeruginosa (Pseudomonasaeruginosa): ATCC9027
B. fungus
Aspergillus niger (Aspergillusniger): ATCC16404
Candida albicans (Candidaalbicans): ATCC10231
3.1.2 for examination culture medium
Fungi culture medium: potato dextrose medium or rose bengal medium, bevel or dull and stereotyped for subsequent use after 115 DEG C of autoclaving 30min.
Bacteria culture media: nutrient agar or lecithin tween 80 nutrient agar, makes flat board after 121 DEG C of autoclaving 20min or inclined-plane is for subsequent use.
3.1.3 the preparation of bacteria suspension
Each antibacterial (staphylococcus aureus, escherichia coli, Pseudomonas aeruginosa) is seeded to nutrient agar slopes respectively, 18h ~ 24h is cultivated in 37 DEG C of constant incubators, add 3ml physiological saline solution piping and druming inclined-plane washed cell, fully after mixing, regulate bacteria suspension concentration to be 1.0 ~ 5.0 × 10
8cfu/ml is for subsequent use.Aspergillus niger and Candida albicans are seeded to potato dextrose medium inclined-plane, in 28 DEG C of constant incubators, cultivate 72h ~ 96h and 24h ~ 48h respectively, add 3ml physiological saline solution piping and druming inclined-plane washed cell, fully mixing makes 1.0 ~ 5.0 × 10
7the bacteria suspension of cfu/ml concentration, for subsequent use in 4 DEG C of storages.
The bacteriostatic test of 3.2 Flos Lonicerae extractive solutions
Adopt disc diffusion method, filter paper card punch is made the circular filter paper sheet of diameter 5mm, be placed in clean small beaker, 121 DEG C of sterilizing 20min put into extracting solution and fully soak after drying.Sterilizing lecithin tween 80 Nutrient agar or rose bengal medium are poured in culture dish respectively, to be cooledly to solidify, draw above-mentioned antibacterial or fungus bacteria suspension 0.1ml is spread evenly across on flat board.Then be affixed on containing on bacterium flat board with the circular filter paper sheet that aseptic nipper gripping was soaked, the equidistant subsides of each flat board 4, wherein using the filter paper of 1 sterilized water immersion as blank, each culture dish is placed in accordingly 37 DEG C or 28 DEG C of constant incubators inversion cultivation 24h or 48h, measure the size of each bacterial strain antibacterial circle diameter under Flos Lonicerae extractive solution, the repetition of 3, each sample, gets its meansigma methods.
Table 2 extract is to the inhibition zone of five kinds of strains
Note: repeat experiment for three times: antibacterial circle diameter≤7mm, fungistatic effect is not obvious; Antibacterial circle diameter >=7mm, fungistatic effect is obvious.
The cosmetic formulations of 4 interpolation Flos Lonicerae extracts
Specific embodiment 1
Extract joins in cosmetics cream frost substrate by table 3
The preservation challenge experimental result of table 4 embodiment 1 and comparative example 1
Table 4 experimental result is known, and Flos Lonicerae extract shows good bacteriostasis in cosmetics cream frost substrate, can be used as pure natural antiseptic and inhibiting bacteria function composition and adds in cosmetics.
Specific embodiment 2
Extract is joined cosmetics essence substrate by table 5
The preservation challenge experimental result of table 6 embodiment 2 and comparative example 2
Essence formula system requires higher to protective system, and table 6 experimental result is known, and Flos Lonicerae extract reveals good bacteriostasis at essence diagram of system, can be used as pure natural antiseptic and inhibiting bacteria function composition and adds in cosmetics.
Industry utilizes probability
The preparation method of Flos Lonicerae extract of the present invention adopts Moisturizing Agent Used in Cosmetic to extract Flos Lonicerae effective bacteriostatic ingredients as solvent, and make extract safe and effective, skin affinity is better, guarantees safety and the effectiveness of extract.Flos Lonicerae extract is used for the research and development of products without anticorrosion cosmetic simultaneously, the use adding alternative chemical preservative of Flos Lonicerae extract, avoid or reduce the untoward reaction that chemical preservative may bring, improve the safety of cosmetics, product gentleness does not stimulate, and extensively can utilize in cosmetic field.
Claims (5)
1. the preparation method of Flos Lonicerae extract: it is characterized in that, comprise after Flos Lonicerae is ground, get and put into steeping tank in right amount and add a certain proportion of cosmetics-stage polyatomic alcohol water solution, the solid-liquid ratio of described Flos Lonicerae and polyatomic alcohol water solution is 1:5-1:60, in polyatomic alcohol water solution, water is 2 ~ 1:1 ~ 4 with polyol quality ratio, stir and soak 8-24 hour, then treatment fluid is proceeded in extractor and carry out microwave extracting, extraction time is 5-40 minute, Extracting temperature is 25-125 DEG C, microwave power is 300-1500W, is filtered by extract and obtains Flos Lonicerae extract.
2. according to claim 1, it is characterized in that Extraction solvent used is propylene glycol, butanediol, glycerol, dipropylene glycol, one or more in pentanediol.
3. the addition of this Flos Lonicerae extract according to claim 1 in cosmetics is 0.1%-30%.
4. according to claim 3, this Flos Lonicerae extract adds in cosmetics, it is characterized in that being applied in cosmetics as antiseptic and inhibiting bacteria function agent.
5. according to claim 3, this Flos Lonicerae extract is used for without in anticorrosion cosmetic, describedly comprises cold cream frost, emulsion, water preparation, essence, mask without anticorrosion cosmetic, and skin-clean cream is white, one in emulsion.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510942149.6A CN105411935A (en) | 2015-12-16 | 2015-12-16 | Preparation method of honeysuckle extract and application of honeysuckle extract to preservative-free cosmetics |
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| CN201510942149.6A CN105411935A (en) | 2015-12-16 | 2015-12-16 | Preparation method of honeysuckle extract and application of honeysuckle extract to preservative-free cosmetics |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265308A (en) * | 2016-08-31 | 2017-01-04 | 南京禾宇化工有限公司 | A kind of Liquor Flos Lonicerae Distillata cervical region essence |
| CN107616931A (en) * | 2017-07-28 | 2018-01-23 | 贾仰胜 | A kind of active hydrating lotion |
| CN108125869A (en) * | 2018-02-01 | 2018-06-08 | 江门职业技术学院 | Dried orange peel general flavone and its application in cosmetics are extracted from dried orange peel using ultrasonic wave auxiliary law |
| CN110628508A (en) * | 2019-10-15 | 2019-12-31 | 深圳波顿香料有限公司 | Preparation method of compound plant extract for promoting production of body fluid and increasing moisture and cold-extraction electronic cigarette oil |
| CN114848553A (en) * | 2022-05-20 | 2022-08-05 | 山东福瑞达生物股份有限公司 | Natural preservative and application thereof |
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| CN103735434A (en) * | 2013-09-29 | 2014-04-23 | 上海莱博生物科技有限公司 | Natural plant compound having anticorrosive effect and daily chemical products |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265308A (en) * | 2016-08-31 | 2017-01-04 | 南京禾宇化工有限公司 | A kind of Liquor Flos Lonicerae Distillata cervical region essence |
| CN107616931A (en) * | 2017-07-28 | 2018-01-23 | 贾仰胜 | A kind of active hydrating lotion |
| CN108125869A (en) * | 2018-02-01 | 2018-06-08 | 江门职业技术学院 | Dried orange peel general flavone and its application in cosmetics are extracted from dried orange peel using ultrasonic wave auxiliary law |
| CN110628508A (en) * | 2019-10-15 | 2019-12-31 | 深圳波顿香料有限公司 | Preparation method of compound plant extract for promoting production of body fluid and increasing moisture and cold-extraction electronic cigarette oil |
| CN114848553A (en) * | 2022-05-20 | 2022-08-05 | 山东福瑞达生物股份有限公司 | Natural preservative and application thereof |
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Application publication date: 20160323 |