CN111265598A

CN111265598A - Composite broad-spectrum antibacterial agent and preparation method thereof

Info

Publication number: CN111265598A
Application number: CN202010134558.4A
Authority: CN
Inventors: 杨淳文
Original assignee: 杨淳文
Priority date: 2020-03-02
Filing date: 2020-03-02
Publication date: 2020-06-12

Abstract

The raw materials for preparing the antibacterial agent comprise, by mass, 10-15 parts of oldenlandia diffusa, 15-25 parts of rosemary leaf, 10-15 parts of Ampelopsis grossedentata tea, 5-10 parts of common andrographis herb, 10-15 parts of honeysuckle, 5-10 parts of golden cypress, 5-10 parts of coptis chinensis, 5-10 parts of common cnidium fruit, 1-10 parts of polyhexamethylene guanidine and 0.2-5 parts of nano silver solution. The antibacterial agent disclosed by the invention is mild in property, free of stimulation, safe and effective, and has remarkable broad-spectrum anti-inflammatory, bactericidal, bacteriostatic and antibacterial effects.

Description

Composite broad-spectrum antibacterial agent and preparation method thereof

Technical Field

The invention relates to the technical field of antibacterial and bactericidal products, in particular to an antibacterial and bactericidal product taking Chinese herbal medicines as main components.

Background

Along with the enhancement of public health consciousness, the demand of disinfection and sterilization agents is increasing, in recent years, various countries focus on finding disinfection and sterilization agents which are harmless to the environment and have quick action, and the disinfection solution has a plurality of varieties, but generally has weak sterilization performance, poor stability, high toxicity, easy skin corrosion and high environmental pollution degree; for example, the conventional 84 disinfectant is a chlorine-containing disinfectant, which has certain irritation and corrosiveness, and foods such as vegetables and fruits are easily poisoned if the 84 disinfectant is used for disinfection. More attention is paid to a hydrogen peroxide and chlorine dioxide gas sterilization method, and the sterilization of ozone also has wide use value.

The disinfectant can be classified into a sterilizing agent, a high-efficiency disinfectant, a middle-efficiency disinfectant and a low-efficiency disinfectant according to the action level. The sterilizing agent can kill all microorganisms to meet the sterilization requirement, and comprises potassium permanganate, formaldehyde, glutaraldehyde, ethylene oxide, peroxyacetic acid, hydrogen peroxide, chlorine dioxide and the like. Most of the existing disinfectants are prepared by matching chemical components, and the chemical components can cause certain damage to human bodies, so that the human bodies can be inevitably damaged after long-term use.

The research on the Chinese herbal medicine antibacterial lotion is very rich. Denglifen et al reported that products prepared from bamboo extract, juniperic acid, quercetin, paucinol and other Chinese medicinal extracts as main ingredients have good antibacterial effects (the antibacterial effect and toxicity test of a Chinese medicinal antibacterial lotion, Denglifen et al, J.Chinesiology, 2008, 25 (3): 268). Patent document with application number of CN201310267817 discloses a Chinese herbal medicine antibacterial lotion, which comprises Chinese mugwort leaf, radix sophorae flavescentis, radix stemonae, fructus cnidii, fructus kochiae, menthol, borneol, lactic acid, RH-40, DTC, CAB-35, glycerol, ethanol, chlorhexidine acetate and the like, and the product can quickly kill pathogenic bacteria and has good anti-inflammatory and antipruritic effects; patent document with application number CN200910247163 discloses a traditional Chinese medicine antibacterial lotion with good sterilizing and itching relieving effects and a preparation process thereof, the traditional Chinese medicine antibacterial lotion is composed of lithospermum, fructus cnidii, plantain herb, radix stemonae, pepper, chlorhexidine acetate and borneol, and mainly kills pathogens such as staphylococcus aureus, candida albicans, escherichia coli and the like which cause gynecological inflammation; patent document with application number CN 200810107417) discloses a traditional Chinese medicine antibacterial lotion with good sterilizing and itching relieving effects and a preparation process thereof, the traditional Chinese medicine antibacterial lotion is composed of radix sophorae flavescentis, fructus cnidii, plantain herb, radix stemonae, pepper, chlorhexidine acetate and borneol, and is mainly used for killing pathogens such as staphylococcus aureus, candida albicans, escherichia coli and the like which cause gynecological inflammation; patent document with application number CN200810073953 discloses a traditional Chinese medicine antibacterial lotion with good sterilizing and itching relieving effects and a preparation process thereof, wherein the traditional Chinese medicine antibacterial lotion is composed of radix sophorae flavescentis, fructus cnidii, fructus forsythiae, pepper and chlorhexidine acetate and mainly kills pathogens such as staphylococcus aureus, candida albicans, escherichia coli and the like which cause gynecological inflammation; patent document CN 200810073954) discloses a traditional Chinese medicine antibacterial lotion with good sterilizing and itching relieving effects and a preparation process thereof, wherein the traditional Chinese medicine antibacterial lotion is composed of radix sophorae flavescentis, fructus cnidii, fructus forsythiae, pepper, chlorhexidine acetate and borneol and mainly kills pathogens such as staphylococcus aureus, candida albicans, escherichia coli and the like which cause gynecological inflammation; patent document CN200810073954 discloses a Chinese medicinal antibacterial lotion with good sterilizing and itching relieving effects and its preparation process, wherein the Chinese medicinal antibacterial lotion is composed of radix Sophorae Flavescentis, fructus Cnidii, fructus forsythiae, fructus Zanthoxyli, chlorhexidine acetate and Borneolum Syntheticum, and mainly kills pathogens such as Staphylococcus aureus, Candida albicans and Escherichia coli which cause gynecological inflammation.

The Chinese herbal medicines used in the Chinese herbal medicine antibacterial lotion are different, and the action effect is respectively very long and is also respectively limited. In the face of rich Chinese medicine treasury, the Chinese medicine lotion has wide development space and large market demand in developing novel Chinese herbal medicine antibacterial lotion with broad-spectrum anti-inflammatory, bactericidal, bacteriostatic and antibacterial effects.

Disclosure of Invention

The invention aims to provide a compound antibacterial agent which has mild, non-irritant, safe and effective properties and has remarkable broad-spectrum anti-inflammatory, bactericidal, bacteriostatic and antibacterial effects. The invention also provides a preparation method of the antibacterial agent.

The technical scheme adopted by the invention is as follows:

a composite broad-spectrum antibacterial agent is prepared from, by mass, 10-15 parts of oldenlandia diffusa, 15-25 parts of rosemary leaves, 10-15 parts of Ampelopsis grossedentata tea leaves, 5-10 parts of common andrographis herbs, 10-15 parts of honeysuckle flowers, 5-10 parts of golden cypress, 5-10 parts of coptis chinensis, 5-10 parts of common cnidium fruits, 1-10 parts of polyhexamethylene guanidine and 0.2-5 parts of nano-silver solution.

The raw materials for preparing the antibacterial agent are preferably 10 parts of oldenlandia diffusa, 15 parts of rosemary leaves, 10 parts of Ampelopsis grossedentata tea, 5 parts of common andrographis herb, 10 parts of honeysuckle, 5 parts of golden cypress, 5 parts of coptis chinensis, 5 parts of common cnidium fruit, 1 part of polyhexamethylene guanidine and 0.2 part of nano silver solution in parts by mass.

The raw materials for preparing the antibacterial agent are preferably 15 parts of oldenlandia diffusa, 25 parts of rosemary leaves, 15 parts of Ampelopsis grossedentata tea, 10 parts of common andrographis herb, 15 parts of honeysuckle, 10 parts of golden cypress, 10 parts of coptis chinensis, 10 parts of common cnidium fruit, 10 parts of polyhexamethylene guanidine and 5 parts of nano silver solution in parts by mass.

The preparation method of the composite broad-spectrum antibacterial agent comprises the following steps:

(1) weighing oldenlandia diffusa, rosemary leaf, maoyan raspberry tea, common andrographis herb, honeysuckle, golden cypress, coptis chinensis and fructus cnidii according to the mass parts, cleaning impurities, drying, crushing and sieving with a 80-mesh sieve respectively, then combining the crushed raw materials, placing the crushed raw materials in a vacuum reaction furnace, adding 75-95% ethanol with the mass concentration of 8-10 times of the weight of the crushed raw materials, heating to 40-60 ℃, soaking for 24-48 hours, carrying out centrifugal filtration, taking filtrate, decoloring the filtrate with active carbon, then sending the filtrate into a UHT sterilizer for high-super-temperature instantaneous sterilization, carrying out sterilization at the sterilization temperature of 100-125 ℃ for 5-10 s, placing the sterilized filtrate in the vacuum reaction furnace, and concentrating the vacuum reaction furnace into paste with the relative density of 1.10-1.25 at the;

(2) diluting polyhexamethylene guanidine with 10-15 times of deionized water, diluting a nano-silver solution with 10-20 times of deionized water, mixing the diluted polyhexamethylene guanidine solution and the nano-silver solution according to the mass ratio of 1:1, and then placing the mixture in an ultrasonic container for ultrasonic homogenization for 10-20 min;

(3) adding the paste prepared in the step (1) into the mixed solution which is subjected to ultrasonic homogenization in the step (2), continuing to homogenize for 30-60min, and then sending the homogenized liquid into a UHT sterilizer for sterilization at the sterilization temperature of 100-125 ℃ for 5-10 s, thus obtaining the composite broad-spectrum antibacterial agent.

The raw materials for preparing the antibacterial agent have the following respective effects:

herba Hedyotidis Diffusae has effects of lung heat cough and asthma, sore throat, intestinal abscess, furuncle, pyocutaneous disease, venomous snake bite, stranguria with heat, edema, dysentery, enteritis, and jaundice due to damp-heat, and has broad-spectrum anticancer properties.

Rosemary has the effects of tranquilizing and allaying excitement, restoring consciousness, and having certain curative effect on dyspepsia and stomachache, can be used for treating various diseases such as insomnia, palpitation, headache, dyspepsia and the like, can be used for treating trauma and arthritis by external application, also has the effects of strengthening heart, promoting metabolism, promoting blood circulation of peripheral blood vessels and the like, is beneficial to strengthening liver function, reducing blood sugar and arteriosclerosis, and helps to treat rheumatalgia and recover the activity of limbs of paralysis.

The Ampelopsis grossedentata tea contains 17 amino acids such as leucine, iso-amino acid, methionine and the like necessary for human bodies and 14 trace elements such as potassium, calcium, iron, zinc, magnesium and the like, and is particularly rich in high-activity flavone and dihydromyricetin. It is rich in more than 19 kinds of bioflavonoids, and the content of total flavonoids can reach as high as 39.25%. Is the highest flavone content in all the plants found at present and is called the king of flavone in the plants. Has antibacterial, antiinflammatory, heat and toxic materials clearing away, analgesic, repercussive, blood lipid reducing, blood pressure lowering, throat moistening, cough relieving, and immunity improving effects. The product has strong killing ability to Staphylococcus aureus, Streptococcus A, Streptococcus B, Diplococcus pneumoniae, and Bacillus influenzae, and can discharge harmful acid phthalein and ketone body, eliminate blood dirt in blood, regulate blood lipid and blood pressure, soften blood vessel, regulate renal function, and enhance renal tubule reabsorption; especially has obvious effect on treating upper respiratory tract infection, acute and chronic pharyngitis and bronchitis.

Herba Andrographitis has effects of diminishing inflammation and removing toxic substance, and can be used for treating various infectious diseases, such as traumatic infection, furuncle, carbuncle, erysipelas, upper respiratory infection, acute and chronic tonsillitis, acute and chronic pharyngolaryngitis, acute and chronic bronchitis, acute bacillary dysentery, acute gastroenteritis, urinary tract infection, endometritis, pelvic inflammatory disease, otitis media, periodontitis, etc.

The honeysuckle contains chlorogenic acid, luteolin glycoside and other pharmacological active ingredients, has strong inhibitory effect on hemolytic streptococcus, staphylococcus aureus and other pathogenic bacteria and upper respiratory infection pathogenic viruses, and can also enhance immunity, resist early pregnancy, protect liver, resist tumor, diminish inflammation, relieve fever, stop bleeding (blood coagulation), inhibit intestinal absorption of cholesterol and the like. Can be combined with other medicines for treating more than 40 diseases such as respiratory tract infection, bacillary dysentery, acute urinary system infection, hypertension, etc.

The phellodendron has better curative effects on damp-heat diarrhea and dysentery, jaundice and dark urine, pruritus vulvae, heat stranguria and pain, beriberi flaccidity cramped, bone steaming and overstrain, night sweat, spermatorrhea, pyocutaneous disease and pyogenic infections, eczema and the like.

Coptis root has the effects of clearing heat and drying dampness, purging fire and removing toxicity, and has good curative effects on damp-heat fullness, vomiting and acid regurgitation, dysentery, jaundice, high fever and coma, hyperactivity of heart-fire, vexation and insomnia, blood heat and hematemesis and epistaxis, conjunctival congestion, toothache, diabetes, carbuncle, furuncle, eczema and the like. Wherein, the wine coptis is good at clearing away the heat of the upper-jiao and can be used for treating conjunctival congestion and aphtha; JIANG HUANG LIAN QING WEI HE ZHI TONG UE, can be used for treating cold and heat accumulation, damp-heat obstruction, distention and fullness, and emesis; yu Huang Lian can soothe liver, harmonize stomach and stop vomiting, and is indicated for liver-stomach disharmony, vomiting and acid regurgitation.

Fructus Cnidii has effects of eliminating dampness, dispelling pathogenic wind, killing parasite, warming kidney and tonifying yang, and can be used for treating sexual impotence, cold womb, leukorrhagia due to cold-dampness, damp arthralgia and lumbago, vulva eczema, trichomonas vaginitis, etc.

The nano silver solution has strong permeability, has good bactericidal effect on deeper tissue infection caused by common bacteria, stubborn bacteria, drug-resistant bacteria and fungi, is rapid and efficient, and can rapidly combine with cell walls/membranes of bacteria to make enzyme lose activity and die.

Polyhexamethylene guanidine (PHMB) is an environment-friendly high-molecular polymer sterilization disinfectant, has the excellent performances of broad sterilization spectrum, low effective concentration, high action speed, stable property and easy water dissolution, can be used at normal temperature, has long-term antibacterial effect, has no side effect, and is non-corrosive, colorless, odorless and non-toxic.

So far, no composite antibacterial agent polymerized by spreading hedyotis herb, rosemary leaf, maoyanmei tea, common andrographis herb, honeysuckle flower, amur corktree bark, coptis root, common cnidium fruit, polyhexamethylene guanidine and nano silver solution is available.

The invention has the following beneficial effects:

1. the invention reasonably combines oldenlandia diffusa, rosemary leaf, Ampelopsis grossedentata tea, common andrographis herb, honeysuckle flower, amur corktree bark, coptis root and common cnidium fruit, extracts respective effective components, adopts polyhexamethylene guanidine solution and nano silver solution as base material solvents, fuses the effective components extracted from the Chinese herbal medicines, fully exerts the drug effects of various Chinese herbal medicine plants, and prepares the broad-spectrum antibacterial agent with the plant antibacterial and anti-inflammatory effects. The antibacterial agent of the invention is different from the current commercial disinfection products, distilled water and alcohols are used as solvents, and polyhexamethylene guanidine solution and nano silver solution are used as solvents. Polyhexamethylene guanidine is a polyguanidine high molecular polymer, can generate ionization in aqueous solution, has strong electropositivity on a hydrophilic group part, adsorbs various bacteria and viruses which are usually electronegative, enters a cell membrane, inhibits the synthesis of intramembrane liposome to cause apoptosis of thalli, achieves the best sterilization effect, and is the most environment-friendly high molecular antibacterial material which is generally accepted at present. The nano silver solution belongs to a non-ionic material, has the functions of sterilizing and resisting virus and degrading bacteria and organic matters under visible light and ultraviolet light, has the functions of strong antibiosis and virus killing under the condition of no light source, and is non-toxic, environment-friendly, low-carbon and free of secondary pollution. The solvent adopted by the invention is used as a nonionic solvent after dissolving the plant extract, so that the lasting antibacterial effect of the product can be greatly enhanced.

2. The invention fully utilizes the characteristics of various plants, scientifically combines and extracts the plants, and then compounds the plants with high molecular polymers and nano materials to obtain the high-efficiency antibacterial agent with cations, according to the principle that opposite charges in physics attract each other, cell walls with negative charges can be attracted by positive charges of the cations, and the high-efficiency antibacterial agent can firmly adsorb and penetrate cell walls of bacteria and mould in use, and destroy a microorganism electron transmission system, a respiratory system and a substance transmission system so as to quickly kill thalli. After entering the cell body, the cell can also react with the hydroxyl of bacteria to coagulate the protein of the bacteria, destroy the activity of the synthetase of the bacterial cells and make the cells lose the division and reproduction capability and die. Under the action of light, the antibacterial agent can also become a catalytic active center to activate oxygen in water and air, generate OH free radicals and active oxygen ions with strong oxidizing power, destroy the reproductive capacity of bacteria in a short time and cause the bacteria to die. When the bacteria lose activity, the compound silver ions in the antibacterial agent can be dissociated from the bacteria, and the aim of lasting antibacterial is achieved by repeating the sterilization activity.

3. The invention also has good anticancer activity to skin cancer cells, can effectively inhibit cancer cell proliferation, induce cancer cell apoptosis, initiate cancer cell cycle arrest, and inhibit cancer cell migration, and also has antibacterial effect to AIDS virus and daily sanitary disinfection, sterilization, bacteriostasis, antibacterial purification.

4. The antibacterial agent disclosed by the invention adopts the compatibility and the synergy of the plant extract, the high-molecular polymer and the nano-silver solution, and can comprehensively kill more than 850 harmful bacteria within a few minutes, thereby achieving the purpose of broad-spectrum antibacterial and bacteriostatic action. Can replace the existing chemical disinfection antibacterial agent, and has better bactericidal effect on deep tissue infection caused by common bacteria, stubborn bacteria, drug-resistant bacteria, biological bacteria and fungi.

5. The antibacterial agent belongs to a green environment-friendly composite antibacterial agent, does not contain harmful substances such as iodine, chlorine, aldehyde and the like, does not discharge wastes in production, and is safe and environment-friendly.

6. The antibacterial agent has rich raw material sources, scientific production process of the antibacterial agent, low loss of effective components and improved antibacterial effect, and can fully extract the effective components in the Chinese herbal medicine plants.

Detailed Description

The present invention will be further described with reference to the following examples.

Example 1

A composite broad-spectrum antibacterial agent is prepared from (by mass parts) herba Hedyotidis Diffusae 10, herba Rosmarini officinalis 15, Ampelopsis grossedentata 10, herba Andrographitis 5, flos Lonicerae 10, cortex Phellodendri 5, Coptidis rhizoma 5, fructus Cnidii 5, polyhexamethylene guanidine 1, and nano-silver solution 0.2.

(1) weighing 10 parts of spreading hedyotis herb, 15 parts of rosemary leaf, 10 parts of Ampelopsis grossedentata tea, 5 parts of common andrographis herb, 10 parts of honeysuckle flower, 5 parts of amur corktree bark, 5 parts of coptis chinensis and 5 parts of common cnidium fruit according to the mass parts, respectively washing impurities of the traditional Chinese medicines with clear water, respectively drying and crushing the traditional Chinese medicines, sieving the traditional Chinese medicines with a 80-mesh sieve, then combining the sieved crushed raw materials, placing the crushed raw materials in a vacuum reaction furnace, adding 80% ethanol with the mass concentration of 10 times of the crushed raw materials by weight, heating the mixture to 40-50 ℃, soaking the mixture for 48 hours, carrying out centrifugal filtration, carrying out decoloration treatment on filtrate by using active carbon, then sending the filtrate into a UHT sterilizer for carrying out high-super-temperature instantaneous sterilization, wherein the sterilization temperature is 100 ℃, the sterilization time is 10 seconds, and placing the sterilized;

(2) diluting polyhexamethylene guanidine with 10 times of deionized water, diluting a nano-silver solution with 15 times of deionized water, mixing the diluted polyhexamethylene guanidine solution and the nano-silver solution according to the mass ratio of 1:1, and then placing the mixture in an ultrasonic container for ultrasonic homogenization for 15 min;

(3) and (3) adding the paste prepared in the step (1) into the mixed liquid which is subjected to ultrasonic homogenization in the step (2), continuing to homogenize for 40min, and then sending the homogenized liquid into a UHT sterilizer for sterilization at the sterilization temperature of 125 ℃ for 5s to obtain the composite broad-spectrum antibacterial agent.

Example 2

The raw materials for preparing the composite broad-spectrum antibacterial agent comprise, by mass, 15 parts of oldenlandia diffusa, 25 parts of rosemary leaves, 15 parts of Ampelopsis grossedentata tea, 10 parts of common andrographis herb, 15 parts of honeysuckle, 10 parts of golden cypress, 10 parts of coptis chinensis, 10 parts of common cnidium fruit, 10 parts of polyhexamethylene guanidine and 5 parts of nano silver solution.

(1) weighing 15 parts of spreading hedyotis herb, 25 parts of rosemary leaf, 15 parts of Ampelopsis grossedentata tea, 10 parts of common andrographis herb, 15 parts of honeysuckle flower, 10 parts of golden cypress, 10 parts of coptis chinensis and 10 parts of common cnidium fruit according to the mass parts, respectively washing impurities of the traditional Chinese medicines with clear water, respectively drying and crushing the traditional Chinese medicines, sieving the traditional Chinese medicines with a 80-mesh sieve, then combining the sieved crushed raw materials, placing the crushed raw materials in a vacuum reaction furnace, adding ethanol with the mass concentration of 95 percent, the weight of which is 9 times that of the crushed raw materials, heating the mixture to about 50 ℃, soaking the mixture for 35 hours, carrying out centrifugal filtration, carrying out decoloration treatment on filtrate by using activated carbon, then sending the filtrate into a UHT sterilizer for carrying out high-super-temperature instantaneous sterilization, wherein the sterilization temperature is 110 ℃, the sterilization time is 8s, and;

(2) diluting polyhexamethylene guanidine with 15 times of deionized water, diluting a nano-silver solution with 20 times of deionized water, mixing the diluted polyhexamethylene guanidine solution and the nano-silver solution according to the mass ratio of 1:1, and then placing the mixture in an ultrasonic container for ultrasonic homogenization for 20 min;

(3) and (3) adding the paste prepared in the step (1) into the mixed solution which is subjected to ultrasonic homogenization in the step (2), continuing to homogenize for 60min, and then sending the homogenized liquid into a UHT sterilizer for sterilization at the sterilization temperature of 110 ℃ for 8s to obtain the composite broad-spectrum antibacterial agent.

Example 3

A composite broad-spectrum antibacterial agent is prepared from, by mass, 10 parts of oldenlandia diffusa, 25 parts of rosemary leaves, 12 parts of Ampelopsis grossedentata tea, 8 parts of common andrographis herb, 15 parts of honeysuckle, 8 parts of golden cypress, 5 parts of coptis chinensis, 7 parts of common cnidium fruit, 5 parts of polyhexamethylene guanidine and 3 parts of nano-silver solution.

(1) weighing 10 parts of spreading hedyotis herb, 25 parts of rosemary leaf, 12 parts of Ampelopsis grossedentata tea, 8 parts of common andrographis herb, 15 parts of honeysuckle, 8 parts of golden cypress, 5 parts of coptis chinensis and 7 parts of common cnidium fruit according to the mass parts, respectively washing impurities of the traditional Chinese medicines with clear water, respectively drying and crushing the traditional Chinese medicines, sieving the traditional Chinese medicines with a 80-mesh sieve, then combining the sieved crushed raw materials, placing the crushed raw materials in a vacuum reaction furnace, adding ethanol with the mass concentration of 85 percent, which is 10 times of the weight of the crushed raw materials, heating the mixture to 50-60 ℃, soaking the mixture for 28 hours, carrying out centrifugal filtration, carrying out decoloration treatment on filtrate by using active carbon, then sending the filtrate into a UHT sterilizer for carrying out high-super-temperature instantaneous sterilization, wherein the sterilization temperature is 125 ℃, the sterilization time is 5 s;

(2) diluting polyhexamethylene guanidine with 10 times of deionized water, diluting a nano-silver solution with 10 times of deionized water, mixing the diluted polyhexamethylene guanidine solution and the nano-silver solution according to the mass ratio of 1:1, and then placing the mixture in an ultrasonic container for ultrasonic homogenization for 15 min;

(3) and (3) adding the paste prepared in the step (1) into the mixed solution which is subjected to ultrasonic homogenization in the step (2), continuing to homogenize for 60min, and then sending the homogenized liquid into a UHT sterilizer for sterilization at the sterilization temperature of 125 ℃ for 8s to obtain the composite broad-spectrum antibacterial agent.

Example 4

A composite broad-spectrum antibacterial agent is prepared from, by mass, 12 parts of oldenlandia diffusa, 15 parts of rosemary leaves, 10 parts of Ampelopsis grossedentata tea, 6 parts of common andrographis herb, 12 parts of honeysuckle, 7 parts of golden cypress, 7 parts of coptis chinensis, 10 parts of common cnidium fruit, 8 parts of polyhexamethylene guanidine and 4 parts of nano-silver solution.

(1) weighing 12 parts of spreading hedyotis herb, 15 parts of rosemary leaf, 10 parts of Ampelopsis grossedentata tea, 6 parts of common andrographis herb, 12 parts of honeysuckle flower, 7 parts of amur corktree bark, 7 parts of coptis chinensis and 10 parts of common cnidium fruit according to the mass parts, washing impurities of the traditional Chinese medicines with clear water respectively, drying and crushing the traditional Chinese medicines respectively, sieving the traditional Chinese medicines with a 80-mesh sieve, then combining the sieved crushed raw materials, placing the crushed raw materials in a vacuum reaction furnace, adding ethanol with the mass concentration of 90 percent, which is 8 times of the weight of the crushed raw materials, heating the mixture to 45-50 ℃, soaking the mixture for 32 hours, carrying out centrifugal filtration, carrying out decoloration treatment on filtrate by using active carbon, then sending the filtrate into a UHT sterilizer for carrying out high-super-temperature instantaneous sterilization, wherein the sterilization temperature is 115 ℃, the sterilization time is;

(2) diluting polyhexamethylene guanidine with 15 times of deionized water, diluting a nano-silver solution with 15 times of deionized water, mixing the diluted polyhexamethylene guanidine solution and the nano-silver solution according to the mass ratio of 1:1, and then placing the mixture in an ultrasonic container for ultrasonic homogenization for 20 min;

(3) and (3) adding the paste prepared in the step (1) into the mixed solution which is subjected to ultrasonic homogenization in the step (2), continuing to homogenize for 40min, and then sending the homogenized liquid into a UHT sterilizer for sterilization at the sterilization temperature of 115 ℃ for 10s to obtain the composite broad-spectrum antibacterial agent.

In the above embodiment, the nano silver solution is preferably a commercially available nano silver simple substance solution, the particle size is 5nm, the concentration of the nano silver solution is less than 50000ppm, and the PH value is neutral. The polyhexamethylene guanidine (PHMB) is preferably commercially available and has the structural formula: C3H 6-NH-C (NH) -NH-C3H 6, colorless to light amber solution, and the effective active ingredient is equal to or larger than 25 percent.

The equipment used for preparing the antibacterial agent, such as a vacuum reaction furnace, centrifugal filtration equipment, sterilization equipment, an ultrasonic container and the like, is the equipment in the prior art. The ultrasonic stirring speed during homogenizing in the ultrasonic container is generally controlled at 12000rpm, the microwave frequency is 2500MHz, and the microwave power is 0.8 Kw. The technical index of the equipment during operation can be adjusted according to the production requirement.

The composite broad-spectrum antibacterial agent prepared by the invention can be diluted according to the ratio of 1:100 for use in conventional use.

The composite broad-spectrum antibacterial agent prepared by the invention has the following antibacterial effects by detection:

detecting item names	Rate of sterilization
		pH value	7.1
Escherichia coli	100％
		Staphylococcus aureus	100％
Candida albicans	100％
		Gonococci	100％
Surface of object by on-site sterilization test	99.9％
		Hepatitis B virus, colibacillus, fungus, foot and mouth disease, anthrax and other bacteria	99.99％
Palm surface for on-site sterilization test	99.99％
		Arsenic (mg/Kg) is less than or equal to 2	Not detected out
Lead (mg/Kg) is less than or equal to 10	Not detected out
		Mercury (mg/Kg) is less than or equal to 1	Not detected out
Cadmium (mg/Kg) is less than or equal to 5	Not detected out

The detection result shows that the composite antibacterial agent has obvious sterilization and disinfection effects on deep tissue infection caused by common bacteria, stubborn bacteria, drug-resistant bacteria, biological bacteria and fungi.

The composite antibacterial agent can also be used for preventing and treating various plant diseases and insect pests in aquaculture and agricultural cultivation. The disinfection and sterilization of public places such as hospitals, schools, office places and the like, pipelines, containers, production facilities and plant floors. Can also be added into spinning, ceramic products, daily cosmetics, paints, plastics and other materials to form antibacterial materials.

Claims

1. The composite broad-spectrum antibacterial agent is characterized in that raw materials for preparing the antibacterial agent comprise, by mass, 10-15 parts of oldenlandia diffusa, 15-25 parts of rosemary leaves, 10-15 parts of Ampelopsis grossedentata tea, 5-10 parts of common andrographis herb, 10-15 parts of honeysuckle, 5-10 parts of golden cypress, 5-10 parts of coptis chinensis, 5-10 parts of common cnidium fruit, 1-10 parts of polyhexamethylene guanidine and 0.2-5 parts of nano silver solution.

2. The composite broad-spectrum antibacterial agent of claim 1, wherein the raw materials for preparing the antibacterial agent comprise, by mass, 10 parts of oldenlandia diffusa, 15 parts of rosemary leaves, 10 parts of maoyanmei tea, 5 parts of common andrographis herb, 10 parts of honeysuckle, 5 parts of golden cypress, 5 parts of coptis chinensis, 5 parts of common cnidium fruit, 1 part of polyhexamethylene guanidine and 0.2 part of nano-silver solution.

3. The composite broad-spectrum antibacterial agent of claim 1, wherein raw materials for preparing the antibacterial agent comprise, by mass, 15 parts of oldenlandia diffusa, 25 parts of rosemary leaves, 15 parts of maoyanmei tea, 10 parts of common andrographis herb, 15 parts of honeysuckle, 10 parts of golden cypress, 10 parts of coptis chinensis, 10 parts of common cnidium fruit, 10 parts of polyhexamethylene guanidine and 5 parts of nano-silver solution.

4. A process for the preparation of a composite broad-spectrum antibacterial agent according to claim 1, 2 or 3, characterized by the following: