CN116745418A - 用于等位基因的rna编码的dna替换的组合物和方法 - Google Patents

用于等位基因的rna编码的dna替换的组合物和方法 Download PDF

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CN116745418A
CN116745418A CN202180088340.2A CN202180088340A CN116745418A CN 116745418 A CN116745418 A CN 116745418A CN 202180088340 A CN202180088340 A CN 202180088340A CN 116745418 A CN116745418 A CN 116745418A
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crispr
type
nucleic acid
protein
cas
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Chinese (zh)
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Y·金
J·M·瓦特斯
A·哈默尔
S·J·拉维特
D·施瓦克
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Pairing Plant Service Co ltd
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Pairing Plant Service Co ltd
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    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
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CN202180088340.2A 2020-11-06 2021-11-05 用于等位基因的rna编码的dna替换的组合物和方法 Pending CN116745418A (zh)

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US202063110386P 2020-11-06 2020-11-06
US63/110,386 2020-11-06
PCT/US2021/058235 WO2022098993A2 (en) 2020-11-06 2021-11-05 Compositions and methods for rna-encoded dna-replacement of alleles

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CN116745418A true CN116745418A (zh) 2023-09-12

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US (1) US20220145334A1 (enExample)
EP (1) EP4240844A2 (enExample)
JP (1) JP2023549339A (enExample)
KR (1) KR20230106633A (enExample)
CN (1) CN116745418A (enExample)
AU (1) AU2021374974A1 (enExample)
CA (1) CA3200521A1 (enExample)
CL (1) CL2023001284A1 (enExample)
IL (1) IL302526A (enExample)
MX (1) MX2023005150A (enExample)
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Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3158555A1 (en) 2019-10-23 2021-04-29 Pairwise Plants Services, Inc. Compositions and methods for rna-templated editing in plants
JP7785002B2 (ja) 2019-11-05 2025-12-12 ペアーワイズ プランツ サービシズ， インコーポレイテッド 対立遺伝子のｒｎａにコードされたｄｎａの置換のための組成物および方法
US20220403475A1 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
WO2023049728A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
WO2023114992A1 (en) * 2021-12-17 2023-06-22 Massachusetts Institute Of Technology Programmable insertion approaches via reverse transcriptase recruitment
US20240200102A1 (en) 2022-12-16 2024-06-20 Pairwise Plants Services, Inc. Fusion proteins comprising an intein polypeptide and methods of use thereof
CN120359295A (zh) 2022-12-21 2025-07-22 成对植物服务股份有限公司 工程化蛋白质和其使用方法
AU2024333800A1 (en) * 2023-08-31 2026-03-12 Basf Agricultural Solutions Us Llc Class 2 type v crispr-cas prime editing
WO2025072453A1 (en) 2023-09-26 2025-04-03 Pairwise Plants Services, Inc. Engineered deaminases, compositions comprising the same, and methods of use thereof
IL327621A (en) 2023-10-09 2026-06-01 Pairwise Plants Services Inc Circular permutants, compositions comprising the same, and methods of use thereof
US20250207154A1 (en) * 2023-12-21 2025-06-26 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
US20260022361A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
WO2026019871A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered cytidine deaminases
US20260117237A1 (en) 2024-10-30 2026-04-30 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
CN120366358B (zh) * 2025-06-25 2025-10-10 中国农业大学 一种毕赤酵母高效基因编辑系统、其构建方法及在高产虫草素工程菌株中的应用

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ221259A (en) 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
US6040504A (en) 1987-11-18 2000-03-21 Novartis Finance Corporation Cotton promoter
ATE112314T1 (de) 1988-05-17 1994-10-15 Lubrizol Genetics Inc Pflanzliches ubiquitinpromotorsystem.
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
ES2187497T3 (es) 1990-04-12 2003-06-16 Syngenta Participations Ag Promotores preferentemente en tejidos.
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
JPH06510187A (ja) 1991-08-27 1994-11-17 ノバルティス　アクチエンゲゼルシャフト 同翅類昆虫に対する殺虫性質を有したタンパク質及び植物保護におけるそれらの用法
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
AU751438B2 (en) 1998-02-20 2002-08-15 Syngenta Limited Pollen specific promoter
AR044724A1 (es) 2000-03-27 2005-10-05 Syngenta Participations Ag Promotores del virus de la rizadura amarilla del cestrum
IL151886A (en) 2000-03-27 2010-11-30 Syngenta Participations Ag Cestrum yellow leaf curling virus promoters
WO2005037993A2 (en) 2003-10-06 2005-04-28 Syngenta Participations Ag Promoters functional in plant plastids
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
US9506077B2 (en) 2012-02-01 2016-11-29 Dow Agrosciences Llc Synthetic chloroplast transit peptides
PT3177644T (pt) 2014-08-05 2021-01-13 MabQuest SA Reagentes imunológicos que se ligam a pd-1
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
AU2017302551B2 (en) * 2016-07-26 2023-04-27 The General Hospital Corporation Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
KR102655021B1 (ko) * 2017-07-11 2024-04-04 시그마-알드리치 컴퍼니., 엘엘씨 표적된 게놈 변형을 개선하기 위한 뉴클레오솜 상호작용 단백질 도메인 사용
US11268092B2 (en) * 2018-01-12 2022-03-08 GenEdit, Inc. Structure-engineered guide RNA
WO2019161290A1 (en) * 2018-02-15 2019-08-22 Sigma-Aldrich Co. Llc Engineered cas9 systems for eukaryotic genome modification
KR102853088B1 (ko) * 2018-05-11 2025-09-02 빔 테라퓨틱스, 인크. 프로그래밍가능한 염기 편집기 시스템을 이용하여 단일염기다형성을 편집하는 방법
KR102959497B1 (ko) * 2018-05-11 2026-05-04 빔 테라퓨틱스, 인크. 프로그래밍가능한 염기 편집기 시스템을 이용하여 병원성 돌연변이를 억제하는 방법
WO2020018166A1 (en) * 2018-07-16 2020-01-23 The Regents Of The University Of Michigan Nuclease-mediated nucleic acid modification
WO2020191241A1 (en) * 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
JP7785002B2 (ja) * 2019-11-05 2025-12-12 ペアーワイズ プランツ サービシズ， インコーポレイテッド 対立遺伝子のｒｎａにコードされたｄｎａの置換のための組成物および方法
WO2021138469A1 (en) * 2019-12-30 2021-07-08 The Broad Institute, Inc. Genome editing using reverse transcriptase enabled and fully active crispr complexes
JP7700222B2 (ja) * 2020-09-24 2025-06-30 コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション 編集効率が向上したプライム編集ベースの遺伝子編集用組成物およびその用途
CA3193099A1 (en) * 2020-09-24 2022-03-31 David R. Liu Prime editing guide rnas, compositions thereof, and methods of using the same
AU2022284804A1 (en) * 2021-06-01 2023-12-07 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof

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