CL2023001284A1 - Composiciones y métodos para el reemplazo de alelos con adn codificado por arn - Google Patents

Composiciones y métodos para el reemplazo de alelos con adn codificado por arn

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Publication number
CL2023001284A1
CL2023001284A1 CL2023001284A CL2023001284A CL2023001284A1 CL 2023001284 A1 CL2023001284 A1 CL 2023001284A1 CL 2023001284 A CL2023001284 A CL 2023001284A CL 2023001284 A CL2023001284 A CL 2023001284A CL 2023001284 A1 CL2023001284 A1 CL 2023001284A1
Authority
CL
Chile
Prior art keywords
methods
rna
compositions
encoded dna
allele replacement
Prior art date
Application number
CL2023001284A
Other languages
English (en)
Spanish (es)
Inventor
Kim Yongjoo
Matthew Watts Joseph
Hummel Aaron
Joshua Lawit Shai
Schwark David
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of CL2023001284A1 publication Critical patent/CL2023001284A1/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/102—Mutagenizing nucleic acids
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
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    • C07K2319/00—Fusion polypeptide
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
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CL2023001284A 2020-11-06 2023-05-03 Composiciones y métodos para el reemplazo de alelos con adn codificado por arn CL2023001284A1 (es)

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US202063110386P 2020-11-06 2020-11-06

Publications (1)

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CL2023001284A1 true CL2023001284A1 (es) 2023-12-22

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US (1) US20220145334A1 (enExample)
EP (1) EP4240844A2 (enExample)
JP (1) JP2023549339A (enExample)
KR (1) KR20230106633A (enExample)
CN (1) CN116745418A (enExample)
AU (1) AU2021374974A1 (enExample)
CA (1) CA3200521A1 (enExample)
CL (1) CL2023001284A1 (enExample)
IL (1) IL302526A (enExample)
MX (1) MX2023005150A (enExample)
WO (1) WO2022098993A2 (enExample)

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US20260022361A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
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