CN116671383A - Method for rapid flower formation of kumquat plants through temperature regulation - Google Patents

Method for rapid flower formation of kumquat plants through temperature regulation Download PDF

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CN116671383A
CN116671383A CN202310892761.1A CN202310892761A CN116671383A CN 116671383 A CN116671383 A CN 116671383A CN 202310892761 A CN202310892761 A CN 202310892761A CN 116671383 A CN116671383 A CN 116671383A
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temperature
plants
kumquat
flower formation
flowering
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徐强
刘兵
王淑婷
饶智雄
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Huazhong Agricultural University
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    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract

本发明属于柑橘育种与栽培领域,具体是一种通过温度调控金柑类植物快速成花的方法,该方法将成年植株转移至温度为34~38℃环境中培养6~10天;将上述温度处理后的植株转移至低于30℃、长日照环境中,等待开花。本发明的方法操作简单,比传统的利用干旱调控金柑类植物开花的效率更高、时间更短,同时可以节约人工成本。利用现代温控技术,该方法可以应用于人工气候箱和大型温室,具有一定的应用前景。

The invention belongs to the field of citrus breeding and cultivation, and specifically relates to a method for rapid flowering of kumquat plants through temperature regulation. In the method, adult plants are transferred to an environment with a temperature of 34-38° C. for 6-10 days; the above-mentioned temperature treatment The final plants are transferred to a place below 30°C and in a long-day environment, waiting for flowering. The method of the invention is simple to operate, has higher efficiency and shorter time than the traditional method of using drought to control the flowering of kumquat plants, and can save labor costs at the same time. Using modern temperature control technology, this method can be applied to artificial climate chambers and large-scale greenhouses, and has certain application prospects.

Description

通过温度调控金柑类植物快速成花的方法The method for fast flowering of kumquat plants by temperature regulation

技术领域technical field

本发明涉及柑橘育种与栽培领域,具体涉及一种通过温度调控金柑类植物快速成花的方法。The invention relates to the field of citrus breeding and cultivation, in particular to a method for quickly forming flowers of kumquat plants through temperature regulation.

背景技术Background technique

柑橘,芸香科柑橘亚科植物,用作经济栽培的有3个属:枳属、金柑属和柑橘属。柑橘在我国栽培历史悠久,除了满足人们的食用需求,金柑、四季桔等金柑类柑橘也可以作为观赏植物,挂果后果实金黄饱满,极具观赏价值。尤其在春节期间,柑橘在中国传统文化中的“吉祥”蕴意更催生了人们对观赏柑橘的消费需求。柑橘的成花习性差异很大,柑橘属植物在亚热带地区大多春季开花一次,而金柑类植物可以连续成花,且花期集中在夏季。柑橘的花期习性不仅限制了柑橘属间杂交育种工作的开展,也不利于观赏柑橘在节日期间的生产。Citrus, Rutaceae Citrus subfamily plants, there are 3 genera used for economic cultivation: Citrus aurantium, Golden Citrus and Citrus. Citrus has a long history of cultivation in my country. In addition to meeting people's food needs, kumquat citrus such as kumquats and quaters can also be used as ornamental plants. After fruiting, the fruits are golden and plump, which is of great ornamental value. Especially during the Spring Festival, the "auspicious" implication of citrus in Chinese traditional culture has given rise to people's consumption demand for ornamental citrus. The flowering habits of citrus are very different. Most citrus plants bloom once in spring in subtropical regions, while kumquat plants can bloom continuously, and the flowering period is concentrated in summer. The flowering habit of citrus not only restricts the development of cross breeding between citrus genera, but also is not conducive to the production of ornamental citrus during festivals.

现有关于诱导柑橘形成花芽的主要措施条件为低温和干旱。金柑、四季桔等金柑类植物可通过自然干旱诱导成花,在生产上也可通过控制灌水和环割等方式来促进花芽分化。但控水处理所需的时间较长,并且操作步骤上较为繁杂,控水程度也需要准确把握,否则过度干旱会引起落叶,影响植株的长势。环割需要大量人工,成本高、效率低,而且会影响树体正常生长发育。The existing main measures for inducing flower bud formation in citrus are low temperature and drought. Kumquat plants such as kumquats and quaters can be induced to flower through natural drought, and flower bud differentiation can also be promoted by controlling irrigation and circumcision in production. However, water control treatment takes a long time, and the operation steps are relatively complicated, and the degree of water control needs to be accurately grasped, otherwise excessive drought will cause leaf fall and affect the growth of plants. Circumcision requires a lot of labor, high cost, low efficiency, and will affect the normal growth and development of the tree.

发明内容Contents of the invention

本发明的目的是为了克服现有诱导金柑类植物成花的栽培措施的不足,提供一种通过较高的温度调控金柑类植物快速成花的方法,仅通过一段时间特定的环境温度处理就可以快速产生花芽,方法易行,操作简便,成本低廉。The purpose of the present invention is to overcome the shortcomings of the existing cultivation measures for inducing kumquat plants to flower, and to provide a method for fast flowering of kumquat plants through higher temperature regulation, only through a period of specific environmental temperature treatment. Quickly produce flower buds, the method is easy to implement, the operation is simple and convenient, and the cost is low.

为实现上述目的,本发明所设计一种通过温度调控金柑类植物快速成花的方法,包括以下步骤:In order to achieve the above object, the present invention designs a method for rapidly forming flowers of kumquat plants by temperature regulation, comprising the following steps:

(1)将成年植株转移至温度为34~38℃环境中培养6~10天;(1) Transfer the adult plants to an environment with a temperature of 34-38° C. for 6-10 days;

(2)将步骤(1)温度处理后的植株转移至低于30℃、长日照环境中,等待开花。(2) Transfer the plants after the temperature treatment in step (1) to an environment lower than 30°C and in long-day sunshine, and wait for flowering.

进一步地,所述步骤(1)中,成年植株为树体健壮、长势良好、已度过童期的植株。Further, in the step (1), the adult plant is a plant with a strong tree body, a good growth condition, and a plant that has passed childhood.

再进一步地,所述步骤(1)中,温度培养处理期间土壤保持湿润,光照模式为人工长日照。Still further, in the step (1), the soil is kept moist during the temperature cultivation treatment, and the illumination mode is artificial long-sunlight.

再进一步地,所述步骤(1)中,保持土壤湿润的方法为每天浇水1次。Still further, in the step (1), the method for keeping the soil moist is to water once a day.

再进一步地,所述步骤(1)中,温度培养处理期间:温度为35℃,培养7天。Still further, in the step (1), during the temperature culture treatment: the temperature is 35° C., and cultured for 7 days.

再进一步地,所述步骤(2)中,培养温度为26~28℃。Still further, in the step (2), the culture temperature is 26-28°C.

再进一步地,所述步骤(2)中,培养温度为26℃。Still further, in the step (2), the culture temperature is 26°C.

再进一步地,所述步骤(1)和步骤(2)中,长日照条件均为光照16h、黑暗8h。Still further, in the step (1) and the step (2), the long daylight conditions are 16 hours of light and 8 hours of darkness.

再进一步地,所述步骤(1)和步骤(2)中,光照强度为:10000Lx。Still further, in the step (1) and step (2), the light intensity is: 10000Lx.

本发明的有益效果:Beneficial effects of the present invention:

本发明仅通过一段时间的特定环境温度处理就可以快速产生花芽进而成花。通过这种方法可以对金柑类植物的开花时间进行人工控制,一方面可以随时满足市场对观赏性柑橘的需求,另一方面可以满足育种工作的需要。与控水、环割调控成花的方式相比,本发明的方法操作简单,可以避免过度干旱对植物生长的影响,同时可以节约人工成本。利用现代温控技术,该方法可以应用于人工气候箱和大型温室,具有一定的应用前景。The present invention can quickly produce flower buds and then form flowers only through a period of specific ambient temperature treatment. The flowering time of kumquat plants can be artificially controlled by this method, on the one hand, the market demand for ornamental citrus can be met at any time, and on the other hand, the needs of breeding work can be met. Compared with water control and circumcision to regulate flower formation, the method of the invention is simple to operate, can avoid the influence of excessive drought on plant growth, and can save labor costs at the same time. Using modern temperature control technology, this method can be applied to artificial climate chambers and large-scale greenhouses, and has certain application prospects.

附图说明Description of drawings

图1为温度处理金柑类植物成花的操作流程示意图;Fig. 1 is the operation flow diagram of temperature treatment kumquat plant flowering;

图中,白色区域代表光照处理,灰色区域代表黑暗处理;In the figure, the white area represents the light treatment, and the gray area represents the dark treatment;

图2为不同温度处理7天后山金柑成花效果统计图;Fig. 2 is a statistical diagram of flowering effect of kumquats after different temperatures are processed for 7 days;

图3为实施例1山金柑35℃处理7天前后成花状的示意图;Fig. 3 is the schematic diagram that becomes flower shape before and after 35 ℃ of processing of embodiment 1 kumquats for 7 days;

图4为实施例2四季桔35℃处理7天后成花状的示意图;Fig. 4 is the schematic diagram that becomes flower shape after 35 ℃ of processing of embodiment 2 Four Seasons oranges for 7 days;

图5为干旱处理山金柑成花的操作流程示意图;Fig. 5 is a schematic diagram of the operation process for drought treatment of kumquat flowering;

图6为山金柑干旱处理前后成花状的示意图;Fig. 6 is the schematic diagram of flowering before and after the drought treatment of kumquat;

图7为山金柑干旱处理后与实施例1山金柑35℃处理7天后成花率对比图。Fig. 7 is a comparison chart of the flowering rate of kumquat after drought treatment and that of kumquat treated at 35°C for 7 days in Example 1.

具体实施方式Detailed ways

下面结合具体实施例对本发明作进一步的详细描述,以便本领域技术人员理解。The present invention will be described in further detail below in conjunction with specific embodiments, so that those skilled in the art can understand.

本发明提供了一种通过温度调控金柑类植物快速成花的方法,包括以下步骤:The present invention provides a kind of method for rapidly forming flowers of kumquat plants by temperature regulation, comprising the following steps:

(1)将成年植株转移至温度为34~38℃环境中培养6~10天;(1) Transfer the adult plants to an environment with a temperature of 34-38° C. for 6-10 days;

(2)将步骤(1)温度处理后的植株转移至低于30℃、长日照环境中,等待开花。(2) Transfer the plants after the temperature treatment in step (1) to an environment lower than 30°C and in long-day sunshine, and wait for flowering.

基于上述方法,使用不同温度处理植株成花的实验Based on the above method, the experiment of using different temperatures to process the flowering of plants

1.1方法1.1 Method

按上述方法不同山金柑在30℃~40℃温度范围内经过7d处理后,观察成花情况;According to the above-mentioned method, after different kumquats were treated for 7 days in the temperature range of 30°C to 40°C, the flowering situation was observed;

1.2结论1.2 Conclusion

如图2所示:在30℃~40℃温度范围内经过7d处理后,34℃~38℃处理范围内促进成花效果最显著,成花率(花芽数/腋芽数)约为60%。因此本发明建议温度处理促进开花的优选温度为34℃~38℃;最优选温度为35℃。As shown in Figure 2: After 7 days of treatment in the temperature range of 30°C to 40°C, the effect of promoting flower formation is the most significant in the temperature range of 34°C to 38°C, and the flower formation rate (number of flower buds/number of axillary buds) is about 60%. Therefore, the present invention suggests that the preferred temperature for temperature treatment to promote flowering is 34°C to 38°C; the most preferred temperature is 35°C.

实施例1Example 1

一种通过温度调控山金柑快速成花的方法,其具体步骤如下:A method for quickly forming flowers by temperature-regulated capers, the specific steps of which are as follows:

(1)选择树体健壮、长势良好且已度过童期的山金柑,移栽到花盆中,待用;(1) select the strong and strong tree body, grow well and have passed the childhood kumquat, transplant in the flowerpot, stand-by;

(2)将步骤(1)所述植株转移至温度为35℃的人工气候箱中培养7天;其中,温度处理期间,每天浇水1次保持土壤湿润,光照模式为人工长日照,长日照条件为光照16h、黑暗8h;10000Lx;(2) Transfer the plants described in step (1) to an artificial climate box with a temperature of 35°C for 7 days; wherein, during the temperature treatment period, water once a day to keep the soil moist, and the lighting mode is artificial long-day sunshine, long-day sunshine The conditions are light 16h, dark 8h; 10000Lx;

(3)将步骤(2)处理后的植株转移至温度为26℃的培养环境中长日照培养,等待开花,其中,长日照条件为光照16h、黑暗8h;10000Lx。(3) Transfer the plants treated in step (2) to a culture environment with a temperature of 26° C. for long-day cultivation and wait for flowering. The long-day conditions are 16 hours of light and 8 hours of darkness; 10000 Lx.

如图3所示:处理前的植株腋芽几乎全部为未分化状态,温度处理后植株的腋芽迅速转变为花芽。As shown in Figure 3: before the treatment, the axillary buds of the plants were almost all in an undifferentiated state, and after the temperature treatment, the axillary buds of the plants transformed into flower buds rapidly.

实施例2Example 2

一种通过温度调控四季桔快速成花的方法,其具体步骤如下:A method for quickly forming flowers by temperature regulation and control of Sijiju, the specific steps of which are as follows:

(1)选择树体健壮、长势良好且已度过童期的四季桔,移栽到花盆中,待用;(1) select the Sijiju which has a strong tree body, good growth and has passed childhood, and is transplanted in a flower pot for use;

(2)将步骤(1)所述植株转移至温度为35℃的人工气候箱中培养7天;其中,温度处理期间,每天浇水1次保持土壤湿润,光照模式为人工长日照,长日照条件为光照16h、黑暗8h;10000Lx;(2) Transfer the plants described in step (1) to an artificial climate box with a temperature of 35°C for 7 days; wherein, during the temperature treatment period, water once a day to keep the soil moist, and the lighting mode is artificial long-day sunshine, long-day sunshine The conditions are light 16h, dark 8h; 10000Lx;

(3)将步骤(2)处理后的植株转移至温度为26℃的培养环境中长日照培养,等待开花,其中,长日照条件为光照16h、黑暗8h;10000Lx。(3) Transfer the plants treated in step (2) to a culture environment with a temperature of 26° C. for long-day cultivation and wait for flowering. The long-day conditions are 16 hours of light and 8 hours of darkness; 10000 Lx.

如图4所示:处理前的植株腋芽几乎全部为未分化状态,温度处理后植株的腋芽迅速转变为花芽。As shown in Figure 4: before the treatment, the axillary buds of the plants were almost all in an undifferentiated state, and after the temperature treatment, the axillary buds of the plants transformed into flower buds rapidly.

根据实际情况,结合实施例1对山金柑的处理,并与现有干旱处理方法进行对比:According to actual situation, in conjunction with the processing of embodiment 1 to capers, and compare with existing drought treatment method:

1.方法1. Method

干旱处理山金柑操作流程如图5所示:在处理前将植株浇透水,等待植株自然干旱;当植株叶片开始卷曲视为干旱处理开始,期间每4~5天少量浇水一次,干旱处理持续时间为4周;干旱处理结束后充分浇水,等待植株开花。The operation process of drought treatment capers is shown in Figure 5: water the plants thoroughly before treatment, and wait for the plants to dry naturally; when the leaves of the plants start to curl, it is regarded as the beginning of drought treatment, during which a small amount of watering is performed every 4 to 5 days, and the drought treatment lasts. The time is 4 weeks; water fully after the drought treatment, and wait for the plants to bloom.

2.结论2. Conclusion

如图6~7所示:干旱处理结束后浇透水,花芽开始发育并开放;干旱处理后成花率约为35%。实施例1的方法通过温度处理山金柑,可快速促进其成花,通过温度处理后成花效果更好,成花率为57%;并且实施例1的方法处理时间在1周左右。现有干旱处理方法时间较长,需要1个月左右;故相对于现有干旱处理的方法相对繁琐,周期长的缺点,本发明方法操作简便,而干旱处理的方法相对繁琐。As shown in Figures 6 to 7: after the drought treatment, the flower buds began to develop and open after watering; after the drought treatment, the flower formation rate was about 35%. The method of Example 1 can quickly promote the flowering of capers by temperature treatment, and the flowering effect is better after temperature treatment, and the flowering rate is 57%; and the treatment time of the method of Example 1 is about 1 week. The existing drought treatment method takes a long time and takes about one month; therefore, compared with the shortcomings of the existing drought treatment method, which are relatively cumbersome and have a long cycle, the method of the present invention is easy to operate, while the drought treatment method is relatively cumbersome.

综上所示:本发明方法通过温度处理后成花效果更好,成花率在50%~60%,而现有干旱处理方法成花率在30%~40%;远远高于干旱处理方法。In summary: the flowering effect of the method of the present invention is better after temperature treatment, and the flowering rate is 50% to 60%, while the flowering rate of the existing drought treatment method is 30% to 40%; far higher than the drought treatment method.

其它未详细说明的部分均为现有技术。尽管上述实施例对本发明做出了详尽的描述,但它仅仅是本发明一部分实施例,而不是全部实施例,人们还可以根据本实施例在不经创造性前提下获得其他实施例,这些实施例都属于本发明保护范围。Other parts not specified in detail are all prior art. Although the foregoing embodiment has described the present invention in detail, it is only a part of the embodiments of the present invention, rather than all embodiments, and people can also obtain other embodiments according to the present embodiment without inventive step, these embodiments All belong to the protection scope of the present invention.

Claims (9)

1. The method for rapid flower formation of kumquat plants through temperature regulation is characterized by comprising the following steps of:
(1) Transferring the adult plants to an environment with the temperature of 34-38 ℃ for culturing for 6-10 days;
(2) Transferring the plants subjected to the temperature treatment in the step (1) to a long-day environment at a temperature lower than 30 ℃ to wait for flowering.
2. The method for rapid flower formation by temperature control of kumquats according to claim 1, wherein: in the step (1), the adult plant is a plant with strong tree body, good growth vigor and long childhood.
3. The method for rapid flower formation by temperature control of kumquat-like plants according to claim 1 or 2, characterized in that: in the step (1), soil is kept moist during the temperature culture treatment, and the illumination mode is artificial long-day illumination.
4. The method for rapid flower formation by temperature control of kumquat-like plants according to claim 3, wherein: in the step (1), the soil is kept moist by watering 1 time a day.
5. The method for rapid flower formation by temperature control of kumquat-like plants according to claim 1 or 2, characterized in that: in the step (1), during the temperature culturing process: the temperature was 35℃and the culture was carried out for 7 days.
6. The method for rapid flower formation by temperature control of kumquats according to claim 1, wherein: in the step (2), the culture temperature is 26-28 ℃.
7. The method for rapid flower formation by temperature control of kumquats of claim 6, wherein: in the step (2), the culture temperature is 26 ℃.
8. The method for rapid flower formation by temperature control of kumquat-like plants according to claim 3, wherein: in the step (1) and the step (2), the long-day conditions are 16h of illumination and 8h of darkness.
9. The method for rapid flower formation by temperature control of kumquats of claim 6, wherein: in the step (1) and the step (2), the illumination intensity is as follows: 10000Lx.
CN202310892761.1A 2023-07-20 2023-07-20 Method for rapid flower formation of kumquat plants through temperature regulation Pending CN116671383A (en)

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