CN116650342A - Collagen nano instant mask of yeast recombinant collagen and preparation method thereof - Google Patents
Collagen nano instant mask of yeast recombinant collagen and preparation method thereof Download PDFInfo
- Publication number
- CN116650342A CN116650342A CN202310672941.9A CN202310672941A CN116650342A CN 116650342 A CN116650342 A CN 116650342A CN 202310672941 A CN202310672941 A CN 202310672941A CN 116650342 A CN116650342 A CN 116650342A
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- skin
- collagen
- yeast recombinant
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 118
- 108010035532 Collagen Proteins 0.000 title claims abstract description 118
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- 238000002360 preparation method Methods 0.000 title abstract description 13
- 239000002121 nanofiber Substances 0.000 claims abstract description 52
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- 239000004094 surface-active agent Substances 0.000 claims abstract description 19
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- 238000000034 method Methods 0.000 claims abstract description 15
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- 108090000637 alpha-Amylases Proteins 0.000 claims description 38
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 38
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- -1 malto-oligosaccharide glucoside Chemical class 0.000 claims description 18
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- 239000000463 material Substances 0.000 claims description 5
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- TUNFSRHWOTWDNC-UHFFFAOYSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 description 3
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 2
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 2
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- LQPLDXQVILYOOL-UHFFFAOYSA-I pentasodium;2-[bis[2-[bis(carboxylatomethyl)amino]ethyl]amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC(=O)[O-])CCN(CC([O-])=O)CC([O-])=O LQPLDXQVILYOOL-UHFFFAOYSA-I 0.000 description 2
- 229940068041 phytic acid Drugs 0.000 description 2
- 235000002949 phytic acid Nutrition 0.000 description 2
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- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- FNPXMHRZILFCKX-KAJVQRHHSA-N prednicarbate Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)COC(=O)CC)(OC(=O)OCC)[C@@]1(C)C[C@@H]2O FNPXMHRZILFCKX-KAJVQRHHSA-N 0.000 description 2
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- 238000010998 test method Methods 0.000 description 2
- 229920003169 water-soluble polymer Chemical class 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
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- 102000001187 Collagen Type III Human genes 0.000 description 1
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- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 108010022355 Fibroins Proteins 0.000 description 1
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- SZYSLWCAWVWFLT-UTGHZIEOSA-N [(2s,3s,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)-2-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxolan-2-yl]methyl octadecanoate Chemical compound O([C@@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@]1(COC(=O)CCCCCCCCCCCCCCCCC)O[C@H](CO)[C@@H](O)[C@@H]1O SZYSLWCAWVWFLT-UTGHZIEOSA-N 0.000 description 1
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- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
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- PYIDGJJWBIBVIA-UYTYNIKBSA-N lauryl glucoside Chemical compound CCCCCCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PYIDGJJWBIBVIA-UYTYNIKBSA-N 0.000 description 1
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- 231100000989 no adverse effect Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
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- 229920000747 poly(lactic acid) Polymers 0.000 description 1
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- 239000004626 polylactic acid Substances 0.000 description 1
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 239000001818 polyoxyethylene sorbitan monostearate Substances 0.000 description 1
- 235000010989 polyoxyethylene sorbitan monostearate Nutrition 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/732—Starch; Amylose; Amylopectin; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/86—Polyethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/596—Mixtures of surface active compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/86—Products or compounds obtained by genetic engineering
Abstract
The application discloses a collagen nanometer instant mask containing yeast recombinant collagen and a preparation method thereof, the mask comprises a non-woven fabric substrate layer and a nanofiber layer, wherein the nanofiber layer is formed on the non-woven fabric substrate layer by adopting an electrostatic spinning method, and the nanofiber layer comprises the following components: 1-30 parts by weight of yeast recombinant collagen and 0.1-5 parts by weight of a surface active substance. The facial mask disclosed by the application can be used for remarkably improving wrinkles of the skin of the corners of eyes, remarkably improving skin elasticity, remarkably improving the whiteness and glossiness of the skin of the corners of eyes, remarkably improving the moisture content of the horny layer of the corners of eyes, and has the effects of weakening fine lines and tail lines of the corners, smoothing and refining the skin, tightening the skin, and moisturizing the skin.
Description
Statement of case division
The application relates to a collagen nanometer instant mask with the application number of 2019110410749 and the application name of 'yeast recombinant collagen' and a preparation method thereof, which are filed in 10 months and 30 days of 2019.
Technical Field
The application relates to a facial mask and a preparation method thereof, in particular to a collagen nanometer instant facial mask containing yeast recombinant collagen and a preparation method thereof.
Background
In the cosmetic market, the mask products for personal care mostly adopt the form of relying on essence to be nonwoven fabrics, but various disadvantages exist in the use of the products. People always break off the novel facial mask so as to meet the consumption demands of the market which are continuously changed.
For example, chinese patent application 201310403704.9 discloses a nanofiber whitening and freckle-removing mask patch, which comprises a spinning solution and cotton cloth, wherein the spinning solution comprises reduced glutathione and antioxidant auxiliary materials as active components; chinese patent 201310366143.X discloses a composite nanofiber membrane and its preparation method and application, comprising dissolving any one of polyvinyl alcohol, polylactic acid, regenerated silk fibroin in an organic solvent to form a spinning solution, then adding polyphenols, ultrasonic treatment, electrostatic spinning, etc.; chinese patent application 201810371233.0 discloses a so-called leave-in full-absorption nanofiber functional mask and a method for preparing the same, the mask comprising: 5 to 50 percent of polysaccharide, modified polysaccharide, water-soluble polymer, 1 to 10 percent of polymer substance with whitening effect, 0.001 to 5 percent of functional substance and 0.02 to 2 percent of essence; chinese patent application 201810053724.0 discloses a preparation method of a nanofiber-based dry mask, which comprises the preparation of a polyvinyl alcohol aqueous solution, a chitosan aqueous solution, a sodium hyaluronate solution and a nutrient solution.
In the prior art, when the nanofiber is prepared by utilizing electrostatic spinning, on one hand, the formed nanofiber is easy to generate the phenomena of agglomeration, nodes, broken filaments, holes and the like, the structure of the nanofiber is seriously influenced, the nursing performance of a facial mask is reduced, and on the other hand, the nutritional ingredients added by the spinning solution often influence the formation of the spinning fiber.
The Chinese patent application 201910052945.0 discloses a collagen nanometer instant mask taking hyaluronic acid as a framework and a preparation method thereof, wherein a nanofiber layer is formed on a non-woven fabric substrate layer by adopting an electrostatic spinning method, and comprises sodium hyaluronate, hydrolyzed collagen and a surface active substance; wherein the average molecular weight of the sodium hyaluronate is 20000-60000, and the average molecular weight of the hydrolyzed collagen is 1000-3000. The technology of the patent application can overcome the defects of the prior art, and the nursing performance of the facial mask is excellent.
The Chinese patent application 201910053466.0 discloses a collagen nanometer instant mask taking pullulanase polysaccharide as a framework and a preparation method thereof, wherein a nanofiber layer is formed on a non-woven fabric substrate layer by adopting an electrostatic spinning method, and the nanofiber layer comprises: 10-60 parts by weight of pullulanase polysaccharide, 5-40 parts by weight of hydrolyzed collagen with lower molecular weight; and 0.1 to 5 parts by weight of a surface active substance; wherein the average molecular weight of pullulanase polysaccharide is 100000-300000, and the average molecular weight of hydrolyzed collagen with lower molecular weight is 1000-3000.
To date, the problem of similarity between the effective care ingredients and the collagen sequence of the human body is not solved in the facial mask products in the prior art.
Therefore, there is a need to provide a novel mask with major care ingredients highly similar to the collagen sequence of the human body, so as to overcome the disadvantages and shortcomings in the prior art.
Disclosure of Invention
The invention aims to provide a facial mask with main care components which are highly similar to human collagen sequences and a preparation method thereof.
In order to achieve the above object, the present invention provides a collagen nano instant mask (hereinafter abbreviated as recombinant collagen nanofiber mask) containing yeast recombinant collagen, the mask comprising a non-woven fabric substrate layer and a nanofiber layer, wherein the nanofiber layer is formed on the non-woven fabric substrate layer by an electrostatic spinning method, and the nanofiber layer comprises:
1-30 parts by weight of yeast recombinant collagen, preferably 1-20 parts by weight of yeast recombinant collagen; and
0.1-5 parts by weight of a surface active substance;
wherein the average molecular weight of the yeast recombinant collagen is 20000-100000, preferably 40000-80000; the surface active substance is water-soluble, has skin care effect, and is favorable for electrostatic spinning.
In the recombinant collagen nanofiber facial mask, the yeast recombinant collagen used in the invention is derived from yeast fermentation products, and compared with animal hydrolyzed collagen, the yeast recombinant collagen is macromolecular collagen peptide, has biological activity, has high sequence similarity with human collagen, has good affinity with human body, can repair skin barriers, promotes cell growth, has better skin wrinkle removing effect than animal hydrolyzed collagen, has no immunogenicity (rejection reaction), and is very safe to human body.
The yeast recombinant collagen adopted by the invention is composed of water-soluble collagen peptide, and has the characteristic structure of collagen: G-X-Y repeating structure, G stands for Glycine (Glycine), Y is based on proline (proline). Most of the molecular weight is in the range of 40-80KD, and the small part of the molecular weight is in the range of 20-40KD. Tests show that compared with other animals such as cattle, sheep, pigs, fish and the like, the yeast recombinant collagen is more similar to human collagen, for example, the sequence homology of the yeast recombinant collagen with the fragment sequence of human type III collagen is close to or reaches 100%, and the full-length sequence homology reaches more than 90%.
Experiments show that the yeast recombinant collagen is different from animal collagen: structurally, yeast recombinant collagen belongs to macromolecular collagen peptide, while animal collagen (gelatin) is a hydrolysis product of small molecules; from the similarity with human collagen sequences, the yeast recombinant collagen has high similarity, while the animal collagen has low similarity; from the biological activity, the yeast recombinant collagen has biological activity, and the animal glue principle has no activity; from the point of view of affinity with human, yeast recombinant collagen has good affinity, while animal collagen has poor affinity; from the skin barrier effect, the yeast recombinant collagen has a skin barrier effect, while the animal collagen has no skin barrier effect; from the aspect of promoting cell growth, the yeast recombinant collagen can promote cell growth rapidly, and the animal collagen has slow or no promoting cell growth; from the aspect of skin wrinkle removal, the yeast recombinant collagen has stronger skin wrinkle removal effect, while the animal collagen has weak skin wrinkle removal effect; from the anti-allergy aspect, the yeast recombinant collagen can form a film to isolate an allergen, and can help the group to repair the dermis layer structure of the skin, but the animal glue principle has no effect and even slightly has sensitization; from the aspect of biological safety hidden trouble, the yeast recombinant collagen has no biological safety hidden trouble, and the animal glue principle possibly has viruses; from the point of view of immunogenicity (rejection), yeast recombinant collagen is free from rejection, whereas animal gums may have rejection.
In the recombinant collagen nanofiber facial mask, the yeast recombinant collagen can form a framework, or form a framework together with other substances, or form a framework by other substances, so that the aim and the technical effect of the invention can be realized. The yeast recombinant collagen can form a skeleton of nanofiber and can also be used as a skin care active ingredient.
In the recombinant collagen nanofiber facial mask, the main function of the used surface active substances is to reduce the surface tension, and any surface active agent is not applicable to the invention; the surface active material of the present invention should be water-soluble, have no adverse effect on skin care, and should not affect electrospinning to form uniform fibers. That is, in the present invention, any surfactant which is water-soluble, has a certain skin care effect, and is advantageous for electrospinning can be used in the present invention.
As a specific embodiment of the present invention, in the recombinant collagen nanofiber facial mask of the present invention, the nanofiber layer may further comprise:
0.1-10 parts by weight of sodium hyaluronate;
wherein the average molecular weight of the sodium hyaluronate used is between 20000 and 60000, preferably between 25000 and 50000; the weight ratio of the yeast recombinant collagen to the sodium hyaluronate can be controlled at 100:1 to 1: between 100, preferably controlled at 100:1 to 10: between 100, more preferably controlled at 100:10 to 10: between 100.
As another embodiment of the present invention, in the recombinant collagen nanofiber facial mask of the present invention, the nanofiber layer may further comprise:
1-25 parts by weight of pullulanase polysaccharide;
wherein the average molecular weight of the pullulanase polysaccharide used is between 100000 and 300000, preferably between 150000 and 250000, for example between 170000 and 230000; the weight ratio of the yeast recombinant collagen to the pullulanase polysaccharide can be controlled at 80:1 to 1:80, preferably controlled at 80:3 to 3:80, more preferably controlled at 80:10 to 10: 80.
As still another embodiment of the present invention, in the recombinant collagen nanofiber facial mask of the present invention, the nanofiber layer may include:
1-20 parts by weight of yeast recombinant collagen;
0.1-10 parts by weight of sodium hyaluronate;
1-20 parts by weight of pullulanase polysaccharide;
wherein, the yeast recombinant collagen: the weight ratio of (sodium hyaluronate + pullulanase polysaccharide) can be controlled at 60:1 to 1:60, preferably between 60:3 to 3: between 60; and sodium hyaluronate: the weight ratio of pullulanase polysaccharide can be controlled at 20:1 to 1: 20.
The electrostatic spinning technology adopted by the invention is a construction technology that a polymer solution overcomes surface tension under the action of high-voltage static electricity, forms a Taylor cone at a spinning nozzle, ejects jet flow at high speed, and is solidified to form a nanofiber structure through mechanical stretching and solvent volatilization.
The nanofiber layer in the facial mask can be a layered substance which is prepared by adopting water-soluble polymers such as yeast recombinant collagen, sodium hyaluronate, pullulanase polysaccharide and the like as matrixes and is formed by fibers with diameters of hundreds of nanometers through an electrostatic spinning technology, has the advantages of large specific surface area, good fiber continuity, small fiber membrane pore diameter, high porosity and the like, has good effects on slow release and protection of active substances, is easy to permeate the stratum corneum of skin to reach the deep layer of the skin, and plays the roles of moisturizing, whitening, delaying aging and the like.
In order to form uniform spinning, prevent the occurrence of a coagulation phenomenon during electrospinning, preferably, in the mask of the present invention, the nanofiber layer further comprises: 0.05 to 5 parts by weight of malto-oligose glucoside and 0.03 to 3 parts by weight of hydrogenated starch hydrolysate, preferably 0.5 to 5 parts by weight of malto-oligose glucoside and 0.3 to 3 parts by weight of hydrogenated starch hydrolysate. The use of malto-oligosaccharide glucoside and hydrogenated starch hydrolysate can improve the use condition of the facial mask of the present invention, which prevents the occurrence of the rubbing phenomenon on the face. In the nanofiber layer of the mask of the present invention, if the ratio of malto-oligosaccharide glucoside and hydrogenated starch hydrolysate used is too low, the above improvement effect is not obvious, but if the ratio is too large, the spinning performance, such as too low fiber strength, reduced skin care effect, etc., is adversely affected.
Preferably, in the mask of the present invention, the nanofiber layer further comprises: 0.1 to 4 parts by weight of polyethylene glycol having a high degree of polymerization, and 0.1 to 2 parts by weight of polyethylene glycol having a low degree of polymerization, preferably 0.1 to 2 parts by weight of polyethylene glycol having a high degree of polymerization, and 0.2 to 2 parts by weight of polyethylene glycol having a low degree of polymerization; wherein the average molecular weight of the polyethylene glycol with high polymerization degree is 250000-350000, and the average molecular weight of the polyethylene glycol with low polymerization degree is 6000-10000.
Polyethylene glycol with high polymerization degree can increase the toughness and the like of the fiber, so that the spinning becomes relatively easy and uniform; the polyethylene glycol with low polymerization degree can further prevent the agglomeration phenomenon in the electrostatic spinning process, and promote the formation of uniform spinning.
Preferably, in the mask of the present invention, polyethylene glycol having a low degree of polymerization is used having an average molecular weight of 7000 to 9000; more preferably, the polyethylene glycol of low degree of polymerization used has an average molecular weight between 7600 and 8600.
In the recombinant collagen nanofiber facial mask of the present invention, the surface active substances used may be, but are not limited to: polyoxyethylene sorbitol ester type emulsifiers such as polysorbate-20, polysorbate-60, polysorbate-80, etc.; sucrose ester emulsifiers, such as sucrose laurate, sucrose stearate; polyglycerol-based emulsifiers such as polyglycerol-10 myristate, polyglycerol-6 caprylate, polyglycerol-6 stearate, and the like; glycoside emulsifiers such as coco glucoside, lauryl glucoside, and the like; polyether or polyether ester emulsifiers such as PEG-40 hydrogenated castor oil, PEG-20 methyl glucal sesquistearate, and the like; etc.
As a specific embodiment of the present invention, in the recombinant collagen nanofiber facial mask of the present invention, the nanofiber layer thereof comprises:
5-25 parts by weight of yeast recombinant collagen;
1-10 parts by weight of sodium hyaluronate;
0.5-5 parts by weight of maltooligosaccharide glucoside;
0.3 to 3 parts by weight of hydrogenated starch hydrolysate;
0.1-2 parts by weight of polyethylene glycol with high polymerization degree;
0.2-2 parts by weight of polyethylene glycol having a low degree of polymerization; and
0.1-2 parts by weight of a surface-active substance.
As another embodiment of the present invention, in the recombinant collagen nanofiber facial mask of the present invention, the nanofiber layer thereof comprises:
5-25 parts by weight of yeast recombinant collagen;
2-20 parts by weight of pullulanase polysaccharide;
0.5-5 parts by weight of maltooligosaccharide glucoside;
0.3 to 3 parts by weight of hydrogenated starch hydrolysate;
0.1-2 parts by weight of polyethylene glycol with high polymerization degree;
0.2-2 parts by weight of polyethylene glycol having a low degree of polymerization; and
0.1-2 parts by weight of a surface-active substance.
In the mask of the present invention, a releasable protective layer may be further included on top of the nanofiber layer. In the design of the product, the final product has three layers, namely, a non-woven fabric is used as a substrate, electrostatic spinning is performed on the non-woven fabric substrate, filaments formed by the electrostatic spinning are middle layers (nanofiber layers), and a coating film is arranged on the middle layers, wherein the coating film can be a PE film or a BOPP film, but static electricity is required to be removed. When the product is used, the fiber layer is not required to be peeled off, and only the outer covering film is required to be uncovered for use; the product can be matched with nutrient solution or not, and can be used by spraying pure water on the face of a consumer.
Further, in the mask of the present invention, the nanofiber layer may include 0.001 to 2 parts by weight of a functional substance such as a preservative such as pentasodium pentetate, phytic acid, silver oxide, etc., or other skin care agent, etc.
On the other hand, in order to achieve the purpose of the invention, the invention also provides a method for preparing the facial mask, wherein the method comprises the following steps:
(1) Preparing spinning solution: adding yeast recombinant collagen and a surface active substance into purified water serving as a solvent, and stirring to form uniform spinning solution, wherein the spinning solution comprises 1-30wt% of yeast recombinant collagen and 0.1-5wt% of surface active substance;
(2) And (3) electrostatic spinning: adding the prepared spinning solution into electrostatic spinning equipment, and carrying out electrostatic spinning by taking non-woven fabrics as a receiving base material;
wherein the average molecular weight of the yeast recombinant collagen is 20000-100000, preferably 40000-80000; the surface active substance is water-soluble, has skin care effect, and is favorable for electrostatic spinning.
In the above method, the spinning solution may further comprise 0.1-10wt% of sodium hyaluronate or 1-25wt% of pullulanase polysaccharide, wherein the average molecular weight of the sodium hyaluronate used is 20000-60000, and the average molecular weight of the pullulanase polysaccharide used is 100000-300000.
Preferably, the spinning solution further comprises: 0.05-5wt% malto-oligose glucoside, 0.03-3wt% hydrogenated starch hydrolysate; more preferably, the spinning solution further comprises 0.1-4wt% of polyethylene glycol with high polymerization degree and 0.1-2wt% of polyethylene glycol with low polymerization degree, wherein the average molecular weight of the polyethylene glycol with high polymerization degree is 250000-350000, and the average molecular weight of the polyethylene glycol with low polymerization degree is 6000-10000.
The electrostatic spinning equipment used in the preparation process can be roller-type needleless electrostatic spinning equipment, and the equipment has the characteristics of large spinning quantity, high production efficiency and uniform spinning.
The mask disclosed by the invention is a dry mask, and efficacy tests show that when the mask disclosed by the invention is used immediately, the elasticity RO value of the canthus skin can be obviously improved, the effect of immediately tightening the canthus skin is obvious, the roughness SEr value of the canthus skin and the smoothness SEsm value of the canthus skin can be obviously improved, and the effects of immediately fading the canthus fine lines and smoothing and fine skin are obvious. The continuous use of the facial mask can obviously improve the texture degree of the canthus skin, the wrinkle of the canthus skin and the smoothness SEsm value of the canthus skin, and has the effects of reducing fine lines and angle tail lines and smoothing and refining the skin; the skin elasticity of the canthus can be obviously improved, and the effect of tightening the skin is achieved; the whitening degree and the glossiness of the canthus skin can be remarkably improved, and the whitening agent has the effect of brightening skin color and brightening skin; can remarkably improve the moisture content of the cornea of eyes, and has the effects of moisturizing and preserving moisture.
The present invention will be further described with reference to the drawings and detailed description which are, however, only illustrative of certain specific embodiments of the invention and are not intended to be limiting.
Drawings
FIG. 1 shows the trend of the change in the skin elasticity RO values immediately before and after use of the product of the present invention;
FIG. 2 shows the rate of change of the skin elasticity RO values immediately before and after use of the product of the present invention;
FIG. 3 shows the trend of change in the SEr value of skin roughness immediately before and after application of the product of the invention;
FIG. 4 shows the rate of change of the values of SEr of skin roughness before and after use of the product of the invention;
FIG. 5 shows the trend of the values of the skin smoothness SEsm immediately before and after the use of the product of the present invention;
FIG. 6 shows the rate of change of the SEsm value of the skin smoothness before and after immediate use of the product of the present invention;
FIG. 7 shows the variation of the Ra value of the skin texture before and after continuous use of the product of the present invention;
FIG. 8 shows the rate of change of the Ra value of skin texture before and after continuous use of the product of the present invention;
FIG. 9 shows the trend of the change in the Rz value of the skin texture before and after continuous use of the product of the present invention;
FIG. 10 shows the rate of change of the skin texture Rz values before and after continued use of the product of the present invention;
FIG. 11 shows the trend of variation of the values of the skin texture Rt before and after continuous use of the product of the invention;
FIG. 12 shows the rate of change of the skin texture Rt values before and after continuous use of the product of the present invention;
FIG. 13 shows the trend of the SEw values of skin wrinkles before and after continuous use of the product of the invention;
FIG. 14 shows the rate of change of SEw values of skin wrinkles before and after continued use of the product of the invention;
FIG. 15 shows the trend of the change in the SEsm value of the skin before and after continuous use of the product of the invention;
FIG. 16 shows the rate of change of the SEsm value of skin smoothness before and after continuous use of the product of the present invention;
FIG. 17 shows the trend of the change in the R2 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 18 shows the trend of the change in the Q1 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 19 shows the rate of change of the R2 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 20 shows the rate of change of the Q1 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 21 shows the trend of the variation of the R0 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 22 shows the trend of the R3 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 23 shows the rate of change of the R0 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 24 shows the rate of change of the R3 value of skin elasticity before and after continuous use of the product of the present invention;
FIG. 25 shows the trend of the value of the whiteness L of the skin before and after continuous use of the product according to the invention;
FIG. 26 shows the rate of change of the value of the skin whiteness L before and after continuous use of the product of the invention;
FIG. 27 shows the trend of change in skin brightness ITA DEG values before and after continuous use of the product of the present invention;
FIG. 28 shows the rate of change of skin brightness ITA DEG values before and after continuous use of the product of the present invention;
FIG. 29 shows the trend of skin gloss before and after continuous use of the product of the present invention;
FIG. 30 shows the rate of change of skin gloss before and after continuous use of the product of the invention;
FIG. 31 shows the trend of the moisture content of the stratum corneum before and after continuous use of the product of the invention;
FIG. 32 shows the rate of change of the moisture content of the stratum corneum before and after continuous use of the product of the invention;
FIG. 33 shows the trend of change in wrinkle grading, as assessed visually before and after continued use of the product of the present invention;
fig. 34 shows the rate of change of wrinkle grading, visual assessment before and after continuous use of the product of the present invention.
Detailed description of the preferred embodiments
Example 1
The mask of the invention is prepared according to the following steps:
(1) Preparing spinning solution: pure water is used as a solvent, yeast recombinant collagen (purchased from Jiangsu Yue biological medicine Co., ltd.), sodium hyaluronate, malto-oligosaccharide glucoside, hydrogenated starch hydrolysate, PEG-7M, PEG-180, polyglycerol-10 myristate, pullulanase polysaccharide and preservative are added, and the mixture is stirred in a constant-temperature water bath at 20-50 ℃ for 2-6 hours until the solution is uniform and has no particles, so that spinning solution containing the following components is obtained: 18wt% of yeast recombinant collagen, 0.2wt% of sodium hyaluronate, 2.0wt% of pullulanase polysaccharide, 2.4wt% of maltooligosaccharide glucoside, 1.4wt% of hydrogenated starch hydrolysate, 0.5wt% of PEG-7M, 0.3wt% of PEG-180, 0.1wt% of polyglycerol-10 myristate, and 1.0wt% of preservative, wherein the average molecular weight of the used sodium hyaluronate is about 30000, the average molecular weight of the used yeast recombinant collagen is about 20-100Kda, the average molecular weight of the used PEG-7M is about 300000, the average molecular weight of the used PEG-180 is about 8000, the molecular weight of the used pullulanase polysaccharide is 200000, and the used preservative is pentasodium pentetate, phytic acid, silver oxide;
(2) And (3) electrostatic spinning: pouring the prepared uniform solution into a trough of electrostatic spinning equipment, controlling the humidity of a spinning environment to be 30% -50%, the spinning distance to be 140mm-220mm, the spinning voltage to be 40KV-85KV, the spinning speed to be 0.1-1.2 r/min and the current to be 0.2-1.5mA, and carrying out electrostatic spinning by taking non-woven fabrics as receiving substrates.
Example 2
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 10wt% of yeast recombinant collagen, 3wt% of sodium hyaluronate, 1.0wt% of pullulanase polysaccharide, 0.5wt% of maltooligosaccharide glucoside, 0.5wt% of hydrogenated starch hydrolysate, 0.5wt% of PEG with high polymerization degree, 5wt% of PEG with low polymerization degree, 5.0wt% of polysorbate-20, and 0.1wt% of preservative, wherein the average molecular weight of the used yeast recombinant collagen is about 40-80Kda, the average molecular weight of the used sodium hyaluronate is about 25000, the molecular weight of the used pullulanase polysaccharide is 250000, the average molecular weight of the used PEG with high polymerization degree is about 250000, and the average molecular weight of the used PEG with low polymerization degree is about 10000.
Example 3
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 5wt% of yeast recombinant collagen, 5wt% of sodium hyaluronate, 5.0wt% of pullulanase polysaccharide, 3wt% of maltooligosaccharide glucoside, 1.7wt% of hydrogenated starch hydrolysate, 1wt% of PEG with high polymerization degree, 2wt% of PEG with low polymerization degree, 0.2wt% of sucrose laurate and 2.0wt% of preservative, wherein the average molecular weight of the yeast recombinant collagen used is about 40-80Kda, the average molecular weight of the sodium hyaluronate used is about 50000, the molecular weight of the pullulanase polysaccharide used is 150000, the average molecular weight of the PEG with high polymerization degree used is about 350000, and the average molecular weight of the PEG with low polymerization degree used is about 9000.
Example 4
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 3wt% of yeast recombinant collagen, 10wt% of sodium hyaluronate, 5wt% of malto-oligose glucoside, 3wt% of hydrogenated starch hydrolysate, 4wt% of PEG with high polymerization degree, 2wt% of PEG with low polymerization degree, 3wt% of coco glucoside, and 1.0wt% of preservative, wherein the average molecular weight of the yeast recombinant collagen used is about 40-80Kda, the average molecular weight of the sodium hyaluronate used is about 55000-60000, the average molecular weight of the PEG with high polymerization degree used is about 280000, and the average molecular weight of the PEG with low polymerization degree used is about 7000.
Example 5
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 25wt% of yeast recombinant collagen, 1.0wt% of pullulanase polysaccharide, 2wt% of malto-oligosaccharide glucoside, 1.2wt% of hydrogenated starch hydrolysate, 0.1wt% of PEG with high polymerization degree, 0.1wt% of PEG with low polymerization degree, 1.5wt% of PEG-40 hydrogenated castor oil and 0.5wt% of preservative, wherein the average molecular weight of the yeast recombinant collagen used is about 40-80Kda, the molecular weight of the pullulanase polysaccharide used is 170000, the average molecular weight of the PEG with high polymerization degree used is about 320000, and the average molecular weight of the PEG with low polymerization degree used is about 7600.
Example 6
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 10wt% of yeast recombinant collagen, 2wt% of sodium hyaluronate, 4wt% of malto-oligosaccharide glucoside, 2.5wt% of hydrogenated starch hydrolysate, 0.3wt% of PEG with high polymerization degree, 0.3wt% of PEG with low polymerization degree, 1.8wt% of polyglycerol-6 caprylate and 0.3wt% of preservative, wherein the average molecular weight of the yeast recombinant collagen used is about 40-80Kda, the average molecular weight of the sodium hyaluronate used is about 33000, and the PEG with high polymerization degree and the PEG with low polymerization degree used are the same as in example 5.
Example 7
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 2wt% of yeast recombinant collagen, 20wt% of pullulanase polysaccharide and 1.2wt% of polyglycerol-6 caprylate, wherein the average molecular weight of the yeast recombinant collagen is about 40-80Kda, and the molecular weight of the pullulanase polysaccharide is 200000.
Example 8
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 12wt% of yeast recombinant collagen, 3wt% of sodium hyaluronate, 3.0wt% of pullulanase polysaccharide, 3wt% of maltooligosaccharide glucoside, 2wt% of hydrogenated starch hydrolysate, 0.8wt% of polyglycerol-6 caprylate and 0.1wt% of preservative, wherein the average molecular weight of the yeast recombinant collagen used is about 40-80Kda, the average molecular weight of the sodium hyaluronate used is about 33000, the average molecular weight of the sodium hyaluronate used is about 39000, and the molecular weight of the pullulanase polysaccharide used is 200000.
Example 9
A mask was prepared in the same manner as in example 1, except that the dope prepared contained the following components: 15wt% of yeast recombinant collagen, 10wt% of sodium hyaluronate, 0.3wt% of PEG with high polymerization degree, 1.3wt% of PEG with low polymerization degree, and 1.8wt% of polyglycerol-6 caprylate, wherein the average molecular weight of the used yeast recombinant collagen is about 40-80Kda, the average molecular weight of the used sodium hyaluronate is about 43000, the average molecular weight of the used PEG with high polymerization degree is about 230000, and the average molecular weight of the used PEG with low polymerization degree is about 7500.
Example 10
A mask was prepared in the same manner as in example 1, except that the mask was further subjected to a step of forming a protective layer of a PE film which was releasable on top of the spun layer (nanofiber layer) and removing static electricity. The final mask has three layers, the non-woven fabric is used as a backing, the electrostatic spinning is performed on the non-woven fabric backing, and the filaments formed by the electrostatic spinning are the middle layer (nanofiber layer).
Mask efficacy test (Beijing industrial and commercial university Chinese cosmetic research center)
The mask prepared by the invention is taken to perform efficacy test in the Chinese cosmetic research center of Beijing industrial and commercial university, and the efficacy test is divided into an instant efficacy test and a 4-week combined efficacy test. The test method and results are as follows:
Efficacy test after immediate use
1.1 detection method
Chinese healthy subjects with canthus wrinkle grade 3-4 (standard of the japan society of cosmetics) were selected for 20, with ages ranging from 35-55 years. Before the test product was used on the face, the test product was used 15mi n, the test product was used 30mi n, the Chuan test product 1h and the test product 2h were used to take images of the face, and the skin elasticity of the canthus, the skin roughness of the canthus and the skin smoothness were analyzed. The evaluation results before and after the product is used are compared by a statistical test method so as to judge whether the statistical difference exists.
1.2 detection sites
VISIA-CR image capture: full face VC 98 image shooting: outside of the canthus
Skin elasticity: skin roughness outside the canthus: outside of the canthus
Skin smoothness: outside of the canthus
1.3 test instruments
Skin elasticity tester Cutometer (Coura & Khazaka, germany)
The instrument testing principle is based on the suction and stretching principle, negative pressure is generated on the surface of the tested skin to suck the skin into a specific testing probe, and the depth of the skin sucked into the testing probe is measured by a non-contact optical testing system. The elastic properties of the skin were determined by MPA software analysis. The higher the values of the RO, R2, R3, Q1 parameters, the closer to 1, representing better skin elasticity.
Vi s ioScan VC98 USB(courage&Khazaka,Germany)
The instrument is tested on the skin surface by a special skin image CCD test system with ultraviolet light source in the probe, thus obtaining the active state image of skin. The black-and-white video signal is input into a digitizer of a test system for processing, and then is input into a computer, and the numerical parameters for evaluating the condition of the active skin surface can be obtained by analyzing by a special active skin surface evaluation software SELS. SFr is a skin roughness parameter. SEsm is a skin smoothness parameter, with lower SEr and SEsm values representing smoother skin.
VISIA-CR(CANFI ELD,Amer ica)
The instrument combines the most advanced digital photographing technology, has various light sources (standard light, UV light, cross polarized light and parallel polarized light) and improves the visual degree of skin analysis.
1.4 detection results
The group of 20 subjects was completed with 20 subjects having an average age of 48.30.+ -. 1.20 years.
1.4.1 skin elasticity RO values
Figures 1 and 2 show the trend and rate of change of the RO values of skin elasticity before and after application of the product of the invention, respectively; the results show that:
after the single use experimental group tests the product 15 min, the eye angle skin elasticity RO value of the subject is improved by 5.98 percent, which is obviously better than that before use (p < 0.05) and is obviously better than that of the control group (p < 0.01);
After the single-use experimental group tests the product 30 min, the eye angle skin elasticity RO value of the subject is improved by 1.550 percent and is obviously better than that of the control group (p < 0.05);
after the single use experimental group tests the product for 1h, the elasticity RO value of the canthus skin of the subject is improved by 4.21 percent and is obviously better than that of the control group (p < 0.001);
after the single use experimental group tests the product for 2 hours, the elasticity RO value of the canthus skin of the subject is improved by 0.26 percent and is obviously better than that of the control group (P < 0.05);
the results show that the single-use experimental group test product can obviously improve the skin elasticity RO value of the canthus and has obvious effect of immediately tightening the canthus skin.
1.4.2 skin roughness SEr value
Figures 3 and 4 show the trend and rate of change of the values of skin roughness SEr, respectively, before and after use of the product of the invention; the results show that:
after testing the product 15 min in the single-use experimental group, the roughness SEr value of the canthus and the skin of the subject is improved by 25.10% (absolute value) and is obviously better than that before use (p < 0.05), better than that of the control group but not obvious;
after testing the product 30 min in the single use experimental group, the subject's canthus skin roughness SEr value was improved by 30.87% (absolute value), significantly better than before use (p < 0.01), better than the control group, but not significantly:
after 1h testing of the product in the single use experimental group, the subject's canthus skin roughness SEr value was improved by 29.89% (absolute value), significantly better than before use (P < 0.001), and significantly better than in the control group (P <0.01};
2h after testing the product in the single use experimental group, the subject's canthus skin roughness SEr value was improved by 13.38% (absolute value) better than the control group, but not significantly:
the results show that the single-use experimental group test product can obviously improve the roughness SEr value of the canthus skin, and has obvious effects of instantly fading canthus fine lines, smoothing and refining the skin.
1.4.3 skin smoothness SEsm value
Figures 5 and 6 show the trend and rate of change of the values of the skin smoothness SEsm, respectively, before and after the use of the product according to the invention; the results show that:
after testing the product 15 min in the single use experimental group, the subject's canthus skin smoothness SEsm value was improved by 7.73% (absolute value), significantly better than before use (p < 0.01), and significantly better than in the control group ((p < 0.01);
after the single use of the test product of 30 min, the subject's canthus skin smoothness SEsm value was improved by 0.16% (absolute value) better than the control group, but not significantly;
after testing the product for 1h in the single use experimental group, the eye angle smoothness SEsm value of the subject is improved by 1.90% (absolute value) better than that of the control group, but not obvious;
after the single use of the experimental group to test the product for 2 hours, the eye angle smoothness SEsm of the subject is improved by 4.42 percent (absolute value) which is obviously better than that before the use (p < 0.05) and better than that of the control group but not obvious;
The results show that the single-use experimental group test product can obviously improve the SEsm value of the smoothness of the canthus skin, and has obvious effects of instantly fading the canthus fine lines and smoothing and refining the skin.
Efficacy test after 4 weeks of continuous use
2.1 detection method
In a similar instant efficacy test method, 20 subjects were photographed with eye angle images before, after 2 weeks and after 4 weeks of facial use of the test product, and the moisture content of the cornea, skin elasticity, skin whiteness, skin brightness, skin glossiness were measured, and skin wrinkles, skin smoothness and skin texture were analyzed, and visual evaluation was performed on the eye angle wrinkles of the subjects by a visual sense evaluator.
2.2 detection site
Visual assessment: outside eye angle visual-CR image capture: full face
Derma Top image capture: outside corner of eye VC 98 image capture: outside of the canthus
Stratum corneum moisture content: skin elasticity outside the canthus: outside of the canthus
Whitening degree of skin: skin brightness outside the canthus: outside of the canthus
Skin gloss: skin texture outside the canthus: outside of the canthus
Skin wrinkle parameters: smoothness of skin outside corner of eyes: outside of the canthus
3.3 test instruments
In addition to the instruments used for the instant efficacy test, the following instruments were included:
corneometer (Courage & Khazaka, germany)
Description: the instrument test probe is based on the principle of capacitance for measurement. The moisture content of the skin surface was analyzed by measuring the skin capacitance, and the measured value was a relative value. Higher values represent higher moisture content of the stratum corneum.
Skin color difference test probe CL 400 and Multi-probe skin test System MPA10 (Coura & Khazaka, germany)
Description: the instrument is intended to measure the resulting reflectance spectrum, i.e. the ratio of total reflected light to total incident light for each wavelength at the object surface, to determine the chromaticity value. The L value of the SCE surface reflection light removal mode is used for representing the whiteness of the skin, and the higher the value is, the more the skin is fair; and calculate the value of ITA DEG to represent the skin brightness, the higher the value, the brighter the skin.
Skin gloss probe GL 200 and Multi-probe skin test System MPA10 (Coura & Khazaka, germany)
Description: the skin gloss tester is internally provided with a 635nm semiconductor diode red laser. The laser beam is irradiated onto the skin surface, and long-term reflection can be detected. The detected light passes through the internal diffractive microstructure and by measuring the beam intensity, the gloss value can be calculated. The higher the number, the more shiny the skin.
Derma TOP(EO-TECH,France)
Description: the apparatus detects wrinkles in the skin using advanced Fringe Projection. Ra is the arithmetic average roughness, the lower the value, the shallower the average depth representing wrinkles; rt is skin roughness, the lower the value, the shallower the maximum depth representing wrinkles; rz is the average roughness of the skin and has a close relationship with the number, thickness and depth of skin wrinkles.
2.4 detection results
The group of 20 subjects was completed with 20 subjects having an average age of 48.30.+ -. 1.20 years.
2.4.1 skin texture Ra value
FIGS. 7 and 8 show the trend and rate of change of the Ra value of skin texture before and after continuous use of the product of the present invention, respectively; the results show that:
after 2 weeks of testing the product using the experimental group continuously, the Ra value of the texture degree of the canthus and the skin of the subject is improved by 1.77% (absolute value) better than that of the control group, but not obvious;
after 4 weeks of testing the product with the experimental group, the texture Ra value of the canthus and the skin of the subject is improved by 5.38% (absolute value), which is significantly better than that before the use (P < 0.001) and significantly better than that of the control group (P < 0.05);
the results show that after the test products are continuously used for 4 weeks, the texture degree of the skin at the corners of eyes can be obviously improved, and the effect of reducing fine wrinkles and angle tail wrinkles is achieved.
2.4.2 skin texture Rz value
FIGS. 9 and 10 show the trend and rate of change of the skin texture Rz values before and after continuous use of the product of the present invention, respectively; the results show that:
after 2 weeks of testing the product using the experimental group continuously, the subject's canthus skin texture Rz value was improved by 2.78% (absolute value), better than the control group, but not significant;
after 4 weeks of continuous use of the test product, the subject's canthus skin texture Rz value was improved by 11.13% (absolute value), significantly better than before use (P < 0.001), and significantly better than the control group (P < 0.001);
the results show that after the test products are continuously used for 4 weeks, the texture degree of the skin at the corners of eyes can be obviously improved, and the effect of reducing fine wrinkles and angle tail wrinkles is achieved.
2.4.3 skin texture Rt values
FIGS. 11 and 12 show the trend and rate of change, respectively, of the skin texture Rt values before and after continuous use of the product of the present invention; the results show that:
after 2 weeks of testing the product using the experimental group continuously, the subject's canthus skin texture Rt value was improved by 3.51% (absolute value), better than the control group, but not significant;
after 4 weeks of continuous use of the test product, the subject's canthus skin texture Rt value was improved by 11.40% (absolute value), significantly better than before use (P < 0.01), and significantly better than the control group (P < 0.05);
The results show that after the test products are continuously used for 4 weeks, the texture degree of the skin at the corners of eyes can be obviously improved, and the effect of reducing fine wrinkles and angle tail wrinkles is achieved.
2.4.4 skin wrinkles SEw value
Figures 13 and 14 show the trend and rate of change, respectively, of the SEw values of skin wrinkles before and after continuous use of the product of the invention; the results show that:
after 2 weeks of continuous use of the test product, the subject's canthus skin wrinkles SEw values improved by 9.27% (absolute value), significantly better than before use (P < 0.05), and significantly better than the control group (P < 0.05);
after 4 weeks of continuous use of the test product, the subject's canthus skin wrinkles SEw values improved by 12.75% (absolute value), significantly better than before use (P < 0.001), and significantly better than the control group (P < 0.01);
the results show that after the test products are continuously used for 4 weeks, the wrinkles of the skin at the corners of eyes can be obviously improved, and the effects of reducing fine lines and angle tail lines are achieved.
2.4.5 skin smoothness SEsm value
Figures 15 and 16 show the trend and rate of change of the skin smoothness SEsm value, respectively, before and after continuous use of the product of the present invention; the results show that:
after 2 weeks of testing the product using the experimental group continuously, the subject's canthus skin smoothness SEsm value was improved by 9.32% (absolute value) better than the control group, but not significant;
After 4 weeks of continuous use of the test product, the subject's canthus skin smoothness SEsm value was improved by 13.58% (absolute value), significantly better than before use (P < 0.001), and significantly better than the control group (P < 0.05);
the results show that after the test products are continuously used for 4 weeks, the SEsm value of the skin smoothness of the corners of the eyes can be obviously improved, and the effect of smoothing and refining the skin is achieved.
2.4.6 skin elasticity (R2, Q1 values)
FIGS. 17 to 20 show the trend and the rate of change of the values of skin elasticity (R2, Q1) before and after continuous use of the product of the present invention, respectively; the results show that:
after the test products are continuously used for 2 weeks, the R2 value of the skin elasticity of the corners of eyes of a subject is improved by 9.53%, the Q1 value of the skin elasticity is improved by 10.30%, the values are obviously better than those before the test products are used (P is less than 0.001), and the values are obviously better than those of a control group (P is less than 0.001);
after the test products are continuously used for 4 weeks, the R2 value of the skin elasticity of the corners of eyes of a subject is improved by 11.60 percent, the Q1 value of the skin elasticity is improved by 12.39 percent, and the values are obviously better than those before the test products are used (P is less than 0.01) and are obviously better than those of a control group (P is less than 0.001);
the results show that the elasticity of the skin of the canthus can be obviously improved after the test products are continuously used for 4 weeks.
2.4.7 skin elasticity (R0, R3 value)
FIGS. 21 to 24 show the trend and the rate of change of the values of skin elasticity (R0, R3) before and after continuous use of the product of the present invention, respectively; the results show that:
after 2 weeks of continuous use of the test product, the subject's canthus skin elasticity R0 value was increased by 4.63% significantly better than before use (p < 0.01) and significantly better than the control group (p < 0.001), and the canthus skin elasticity R3 value was increased by 6.26% significantly better than before use (p < 0.001) and significantly better than the control group (p < 0.001);
after 4 weeks of continuous use of the test products, the eye angle skin elasticity R0 value of the subject is 8.42 percent of the capture rise, the skin elasticity R3 value is 6.48 percent of the rise, the values are all obviously better than those before use (p < 0.01), and are all obviously better than those of the control group (p < 0.001);
the results show that after the test products are continuously used for 4 weeks, the elasticity of the skin of the canthus can be obviously improved, and the effect of tightening the skin is achieved.
2.4.8 skin whitening L value
FIGS. 25 and 26 show the trend and rate of change of the value of the fair L of the skin before and after continuous use of the product of the present invention, respectively; the results show that:
after 2 weeks of testing the products in the continuous use experimental group, the canthus skin of the subject is improved by 0.26% from the philosophy L value, which is obviously better than before use (p < 0.05) and is obviously better than that in the control group (p < 0.01);
After 4 weeks of continuous use of the test products in the experimental group, the whitening degree L value of the canthus skin of the subject is improved by 0.62 percent, which is obviously better than that before use (p < 0.05) and is obviously better than that in the control group (p < 0.001);
the results show that after the products are tested for 4 weeks by using the experimental group continuously, the whiteness of the skin of the canthus can be obviously improved, and the effect of brightening the skin color is achieved.
2.4.9 skin Brightness ITA value
FIGS. 27 and 28 show the trend and rate of change of the skin brightness ITA DEG values before and after continuous use of the product of the present invention, respectively; the results show that:
after 2 weeks of testing the product in the continuous use experimental group, the brightness ITA degree value of the canthus skin of the subject is improved by 0.88 percent, which is obviously better than that before use (p < 0.05) and better than that in the control group (p < 0.01);
after 4 weeks of continuous use of the test product, the subject's canthus skin brightness ITA ° value was increased by 2.62%, significantly better than before use (p < 0.01), and significantly better than the control group (p < 0.001));
the results show that after the test products are continuously used for 4 weeks, the test products can be used for displaying tea to improve the brightness of the skin of the canthus, and the test products have the effect of improving the dark yellow skin.
2.4.10 skin gloss
FIGS. 29 and 30 show the trend and rate of change of skin gloss before and after continuous use of the product of the present invention, respectively; the results show that:
After 2 weeks of testing the product in the continuous use experimental group, the glossiness of the skin of the canthus of the subject is improved by 15.13%, which is obviously better than that before the use (p < 0.001) and is obviously better than that in the control group (p < 0.001);
after 4 weeks of continuous use of the test product, the glossiness of the skin at the canthus of the subject is improved by 20.95%, which is obviously better than that before use (p < 0.01) and is obviously better than that of the control group (p < 0.001);
the results show that after the test products are continuously used for 4 weeks, the glossiness of the skin of the canthus can be obviously improved, and the effect of brightening the skin is achieved.
2.4.11 moisture content of stratum corneum
FIGS. 31 and 32 show the trend and rate of change, respectively, of the moisture content of the stratum corneum before and after continuous use of the product of the invention; the results show that:
after 2 weeks of testing the product with the experimental group, the moisture content of the cornea of eyes of the subject is improved by 9.57%, which is significantly better than that before the use (P < 0.001) and significantly better than that of the control group (P < 0.001);
after 4 weeks of testing the product with the experimental group, the moisture content of the cornea of eyes of the subject is improved by 10.56%, which is significantly better than that before the use (p < 0.05) and significantly better than that of the control group (p < 0.001);
the results show that after the test products are continuously used for 4 weeks, the moisture content of the cornea of the eyes can be obviously improved, and the effects of moisturizing and moisturizing are achieved.
2.4.12 visual assessment results and statistical analysis results
FIGS. 33 and 34 show the trend and rate of change of the wrinkle grading, respectively, of the visual assessment before and after continuous use of the product of the present invention; the results show that:
after 2 weeks of testing the product with the experimental group in succession, the subject's canthus wrinkles improved by 6.06% (absolute value), significantly better than before use (p < 0.05), and significantly better than the control group (p < 0.05);
after 4 weeks of testing the product with the experimental group in succession, the subject's canthus wrinkles improved by 13.64% (absolute value), significantly better than before use (p < 0.01), and significantly better than the control group (p < 0.01);
the results show that the test results have the effect of reducing fine lines and fish tail lines after the test results are continuously used for 4 weeks.
Claims (10)
1. The instant collagen nanometer facial mask containing the yeast recombinant collagen is a dry facial mask, and comprises a non-woven fabric base material layer and a nanofiber layer, wherein the nanofiber layer is formed on the non-woven fabric base material layer by adopting an electrostatic spinning method, and the nanofiber layer comprises the following components:
1-25 parts by weight of pullulanase polysaccharide;
1-30 parts by weight of yeast recombinant collagen; and
0.1-5 parts by weight of a surface active substance;
wherein the average molecular weight of pullulanase polysaccharide is 100000-300000;
Wherein the yeast recombinant collagen is composed of water-soluble collagen peptide and has a G-X-Y repetitive structure characteristic of collagen, wherein G represents glycine, X, Y represents amino acid, and the average molecular weight of the yeast recombinant collagen is 40000-80000;
wherein, the weight ratio of the yeast recombinant collagen to the pullulanase polysaccharide is 80:3 to 3: 80;
wherein the surface active substance is water-soluble, has skin care effect and is favorable for electrostatic spinning;
wherein, the spinning solution for forming the nanofiber layer adopts purified water as a solvent.
2. The mask of claim 1, wherein the nanofiber layer further comprises:
0.1-10 parts by weight of sodium hyaluronate;
wherein the average molecular weight of the sodium hyaluronate is 20000-60000;
wherein, the weight ratio of the yeast recombinant collagen to the sodium hyaluronate is 100:1 to 1: between 100.
3. The mask of claim 1, wherein the nanofiber layer comprises:
1-20 parts by weight of yeast recombinant collagen;
0.1-10 parts by weight of sodium hyaluronate;
1-20 parts by weight of pullulanase polysaccharide;
Wherein the average molecular weight of the sodium hyaluronate is 20000-60000, and the average molecular weight of the pullulanase polysaccharide is 100000-300000;
wherein, the yeast recombinant collagen: (sodium hyaluronate+pullulanase polysaccharide) at a weight ratio of 60:3 to 3: 60.
4. A mask as claimed in any one of claims 1 to 3, wherein the nanofiber layer further comprises:
0.05-5 parts by weight of malto-oligosaccharide glucoside; and
0.03-3 parts by weight of hydrogenated starch hydrolysate.
5. A mask as claimed in any one of claims 1 to 3, wherein the nanofiber layer further comprises:
0.1 to 4 parts by weight of polyethylene glycol having a high degree of polymerization;
0.1-2 parts by weight of polyethylene glycol having a low degree of polymerization;
wherein the average molecular weight of the polyethylene glycol with high polymerization degree is 250000-350000, and the average molecular weight of the polyethylene glycol with low polymerization degree is 6000-10000.
6. The mask of claim 2, wherein the nanofiber layer of the mask comprises:
1-25 parts by weight of pullulanase polysaccharide;
5-25 parts by weight of yeast recombinant collagen;
1-10 parts by weight of sodium hyaluronate;
0.5-5 parts by weight of maltooligosaccharide glucoside;
0.3 to 3 parts by weight of hydrogenated starch hydrolysate;
0.1-2 parts by weight of polyethylene glycol with high polymerization degree;
0.2-2 parts by weight of polyethylene glycol having a low degree of polymerization; and
0.1-2 parts by weight of a surface-active substance.
7. The mask of claim 1, wherein the nanofiber layer of the mask comprises:
5-25 parts by weight of yeast recombinant collagen;
2-20 parts by weight of pullulanase polysaccharide;
0.5-5 parts by weight of maltooligosaccharide glucoside;
0.3 to 3 parts by weight of hydrogenated starch hydrolysate;
0.1-2 parts by weight of polyethylene glycol with high polymerization degree;
0.2-2 parts by weight of polyethylene glycol having a low degree of polymerization; and
0.1-2 parts by weight of a surface-active substance.
8. A method of preparing a mask according to any one of claims 1 to 7, wherein the method comprises the steps of:
(1) Preparing spinning solution: taking purified water as a solvent, adding pullulanase polysaccharide, yeast recombinant collagen and a surface active substance, and stirring to form a uniform spinning solution, wherein the spinning solution comprises 1-25wt% of pullulanase polysaccharide, 1-30wt% of yeast recombinant collagen and 0.1-5wt% of surface active substance;
(2) And (3) electrostatic spinning: adding the prepared spinning solution into electrostatic spinning equipment, and carrying out electrostatic spinning by taking non-woven fabrics as a receiving base material;
Wherein the average molecular weight of pullulanase polysaccharide is 100000-300000;
wherein the yeast recombinant collagen is composed of water-soluble collagen peptide and has a G-X-Y repetitive structure characteristic of collagen, wherein G represents glycine, X, Y represents amino acid, and the average molecular weight of the yeast recombinant collagen is 40000-80000;
wherein, the weight ratio of the yeast recombinant collagen to the pullulanase polysaccharide is 80:3 to 3: 80;
wherein the surface active substance is water-soluble, has skin care effect and is favorable for electrostatic spinning.
9. The method of claim 8, wherein the spinning solution further comprises 0.1-10wt% of sodium hyaluronate, wherein the sodium hyaluronate has an average molecular weight of 20000-60000.
10. The method of claim 9, wherein the spinning solution further comprises: 0.05-5wt% of malto-oligose glucoside, 0.03-3wt% of hydrogenated starch hydrolysate and/or 0.1-4wt% of polyethylene glycol with high polymerization degree and 0.1-2wt% of polyethylene glycol with low polymerization degree, wherein the average molecular weight of the polyethylene glycol with high polymerization degree is 250000-350000, and the average molecular weight of the polyethylene glycol with low polymerization degree is 6000-10000.
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| CN111840160B (en) * | 2020-08-11 | 2022-02-25 | 广州鹰远生物科技有限公司 | A composition containing lignum sappan extract and its application in skin external preparation |
| CN112915034A (en) * | 2020-12-07 | 2021-06-08 | 诺斯贝尔化妆品股份有限公司 | Silk fibroin nanofiber instant mask and preparation method thereof |
| CN115120530A (en) * | 2022-06-20 | 2022-09-30 | 诺斯贝尔化妆品股份有限公司 | Dry instant membrane based on electrostatic spinning and preparation method thereof |
| CN114886792A (en) * | 2022-06-30 | 2022-08-12 | 水羊化妆品制造有限公司 | Wrinkle-removing composition, preparation method and application thereof |
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| 侯增淼等: "重组人源性胶原蛋白的制备及表征", 生物技术与方法, vol. 35, no. 2, 25 February 2019 (2019-02-25) * |
| 娱乐FM资讯: "医美面膜如何选才能不踩雷？以下4要素缺一不可", Retrieved from the Internet <URL:http://mp.yulefm.com/beauty/2019-08-26/280094.html> * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20220080175A (en) | 2022-06-14 |
| WO2021082391A1 (en) | 2021-05-06 |
| CN110664619A (en) | 2020-01-10 |
| JP2023500191A (en) | 2023-01-05 |
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