CN114344174B - Mask prepared based on jellyfish collagen solution and preparation method thereof - Google Patents

Abstract

The invention discloses a mask made based on jellyfish collagen, which comprises mask liquid and mask cloth, wherein the mask liquid comprises the following components in parts by weight: 11.58-25.25 parts of basic mask liquid; 3-8 parts of jellyfish collagen solution; 0.5-3 parts of fibronectin solution. The invention also discloses a preparation method of the mask based on jellyfish collagen. The mask adopting the jellyfish collagen can improve the wrinkle removing effect, the moisturizing effect and the skin barrier repairing of the mask.

Description

Mask prepared based on jellyfish collagen solution and preparation method thereof

Technical Field

The invention relates to the technical field of mask preparation, in particular to a mask prepared based on jellyfish collagen solution and a preparation method thereof.

Background

In modern society with increasingly higher living standards, people are more focused on the pursuit of beauty while pursuing better living quality. However, with the rapid development of society, modern people have a fast work rhythm, a large work pressure, irregular living habits, unhealthy and the like, which greatly conflict with the desire of people to pursue beauty. Cosmetics are one of the most convenient methods for improving the external beauty of human bodies, so that the demands of people on the cosmetics are increasing.

With the recent development of biotechnology, more biological raw materials are applied to cosmetics, wherein various peptides or proteins are taken as representative raw materials, and collagen is a widely and mature type of raw materials, and the collagen has great market value because of the effects of moisturizing, filling skin, resisting aging and the like, which are always focused by wide skin care lovers in beauty. The collagen can be extracted from various animal tissues, such as pigskin, beef bone, etc., and the raw materials are convenient to obtain. The recombinant collagen can be obtained by utilizing gene recombination and microorganism fermentation technology, and has the advantages of high purity and strong editability. However, there has been a relatively large dispute from traditional animal (pig, cow, etc.) extraction protocols, such as religious limitations or BSE/TSE risk limitations. The scheme of acquiring collagen by bioengineering has relatively high technical threshold, and the time and cost for early investment are higher.

The jellyfish as marine organism contains a large amount of collagen in the body, and the sources are wide, so that the defects of the two methods can be avoided, and the jellyfish collagen has a huge application prospect in skin care products.

Disclosure of Invention

According to the invention, researches show that the wrinkle removing effect, the moisturizing effect and the skin barrier repairing of the mask can be improved by compounding the fibronectin solution and the jellyfish collagen solution. Accordingly, a first object of the present invention is to provide a mask made based on jellyfish collagen. The second object of the invention is to provide a method for preparing the mask.

In order to achieve the above purpose, the present invention adopts the following technical scheme:

as a first aspect of the present invention, a mask made based on jellyfish collagen includes a mask liquid and a mask cloth, wherein the mask liquid includes the following components in parts by weight:

11.58-25.25 parts of basic mask liquid;

3-8 parts of jellyfish collagen solution;

0.5-3 parts of fibronectin solution.

According to the invention, the mask solution further comprises deionized water, and the basic mask solution, the jellyfish collagen solution and the fibronectin solution comprise the following components in parts by weight:

11.58-25.25 parts of basic mask liquid;

3-8 parts of jellyfish collagen solution;

0.5-6 parts of fibronectin solution;

58.3-81.82 parts of deionized water.

According to the invention, the basic mask liquid comprises the following components in parts by weight:

0.3-0.6 parts of xanthan gum;

5-10 parts of glycerol;

3-5 parts of butanediol;

0.05-0.1 part of sodium hyaluronate;

0.03 to 0.15 of beta-glucan;

1-3 parts of water-soluble jojoba grease;

0.2-0.5 parts of aloe vera extract;

1-3 parts of sodium polyglutamate;

1-3 parts of epigallocatechin gallate;

0.05-0.15 part of Ningxia wolfberry extract;

2-5 parts of lentinus edodes mycelium extract;

1-3 parts of peach resin extract;

sodium chloride 0.05-0.2 parts.

According to the invention, the mask cloth is a seaweed film.

According to the invention, the amino acid sequence of fibronectin in the fibronectin solution is shown as SEQ ID NO. 1.

Further, the concentration of fibronectin in the fibronectin solution is 500ppm.

Preferably, the jellyfish collagen solution is prepared by the following preparation method:

step one, preparing collagen sponge: extracting collagen sponge from jellyfish;

and step two, dissolving the collagen sponge into a 50% -75% hydroxyethyl urea solution with the pH value adjusted to 3.5-4.5 by citric acid.

Further, the preparation of the collagen sponge in the first step comprises the following steps:

A. desalting jellyfish;

B. lipid removal substances: adding a delipase, soaking, and washing off the delipase;

C. c, adding citric acid, homogenizing the material subjected to degreasing in the step B, transferring, and then adding pepsin solution for enzyme digestion;

D. after digestion, centrifugally separating undigested tissue blocks, neutralizing the separated supernatant with alkali liquor to be neutral, and centrifugally collecting collagen gel precipitate;

E. dissolving collagen with citric acid, regulating the supernatant to neutrality, and centrifuging to collect gel precipitate; repeating the steps for more than or equal to N times, wherein N is a natural number; then, the collagen sponge is put into a freeze dryer for freeze drying, and the collagen sponge is prepared.

Preferably, the step E is repeated 6 times from dissolution of citric acid to centrifugation to collect the gel precipitate.

Further, the concentration of the citric acid in the step C is 0.1-1.5 mol/L; the concentration of pepsin is 2-20 mg/g.

Preferably, the concentration of the citric acid in the step C is 0.5mol/L; the concentration of pepsin was 10mg/g.

Further, the alkali liquor in the step D is sodium hydroxide or potassium hydroxide, and the supernatant is neutralized and separated by the alkali liquor until the pH value is 7.0.

Further, the citric acid dosage in the step E is 5-10 times of the precipitation volume, and the concentration is 0.01-0.1 mol/L.

Preferably, the citric acid is used in step E in an amount of 8 times the volume of the precipitate and in a concentration of 0.05mol/L.

As a second aspect of the present invention, a method for preparing the mask liquid described above includes the steps of:

the first step, the following materials are prepared according to the parts by weight: xanthan gum, glycerin, butylene glycol, sodium hyaluronate, beta-glucan, water-soluble jojoba ester, aloe vera extract, sodium polyglutamate, epigallocatechin gallate, lycium barbarum extract, shiitake mushroom hypha extract, peach resin extract, sodium chloride, jellyfish collagen solution and fibronectin solution;

and secondly, weighing xanthan gum, glycerin, butanediol, sodium hyaluronate and beta-glucan, mixing uniformly, adding deionized water preheated to 80-85 ℃, and stirring until all materials are dissolved and dispersed uniformly.

And secondly, cooling to below 60 ℃, adding water-soluble jojoba, aloe vera extract, sodium polyglutamate, lycium barbarum extract, epigallocatechin gallate, shiitake mushroom hypha extract, peach resin extract and sodium chloride, and stirring until all materials are dissolved and dispersed uniformly.

And thirdly, cooling to below 45 ℃, adding the jellyfish collagen solution and the fibronectin solution, and fully stirring until the solution is uniformly dispersed, thus finishing the preparation of the mask liquid.

Fourth, sampling and detecting indexes such as microorganisms and heavy metals, transferring to a mask filling machine to fill a mask bag containing seaweed membrane cloth after the indexes are qualified, and finishing the manufacturing of the mask after sealing.

The mask prepared based on jellyfish collagen has the beneficial effects that: the skin-care gel has the effects of long-acting moisturizing, moisturizing and smoothing skin, filling and removing wrinkles, and other components compounded by the gel also provide the function of repairing skin injury and help the skin to recover a healthier state. The concrete steps are as follows:

1. the fibronectin solution and the jellyfish collagen solution are compounded to improve the wrinkle removing effect, the moisturizing effect and the skin barrier repairing effect of the mask, and play a role in synergy.

2. The jellyfish collagen solution prepared by the method can be stably stored, and has good effects of filling skin and reducing skin moisture loss. In addition, the basic mask liquid forms a complete moisturizing system, and provides a good skin moisturizing scheme for users. The water-soluble jojoba ester is added to form a grease film to cover for prolonging the moisturizing time and providing the effects of moisturizing and smoothing the skin. .

3. The fibronectin of the present invention provides skin repair, stabilizing the stratum corneum effect of the skin. The fibronectin compounded jellyfish collagen can further improve the skin repairing effect, effectively repair the skin barrier and improve the sensitive muscle problem. And the combination of the fibronectin solution and the jellyfish collagen solution has a good effect in smoothing out the dry streak fine streak formed by the dry skin of the nutrition loss.

Drawings

Figure 1 is an average of skin moisture content for each group at various times.

Figure 2 shows the best results (maximum c value) of the wrinkle-removing group a.

Figure 3 shows the best results (maximum c value) of the wrinkle removal group B.

Figure 4 is the best results of a barrier repair group a experiment.

Figure 5 is the best results of a barrier repair group B experiment.

Detailed Description

The present invention will be described in further detail with reference to specific examples. It should be understood that the following examples are illustrative of the present invention and are not intended to limit the scope of the present invention.

1. Materials of this example:

(1) Fibronectin solution: the amino acid sequence of fibronectin is shown as SEQ ID NO. 1; the fibronectin concentration was 500ppm.

(2) Seaweed membrane: after the mask liquid containing sodium ions is soaked, the mask cloth can gel, so that the mask cloth is more attached to the face and has stronger moisture retention and more comfort (can be purchased from Hunan Mei Ri Jiebao non-woven fabrics Co., ltd.).

(3) Xanthan gum (commercially available, daily chemical grade); water-soluble jojoba fat (commercially available, daily chemical grade); glycerol (commercially available, daily chemical grade); butanediol (commercially available, daily chemical grade); aloe vera extract (purchased from the western-style first grass organism); sodium polyglutamate (purchased from baozhou boy for refinement); ningxia wolfberry extract (from Shanxi Senlang organisms); epigallocatechin gallate (available from n-thousand chemical industries, guangzhou); shiitake mushroom hypha extract (purchased from n thousand chemicals, guangzhou); beta-glucan (purchased from Sichuan siraitia); peach resin extract (from Shenzhen Mei Jian organism); sodium hyaluronate (commercially available, daily chemical grade); sodium chloride (commercially available, daily chemical grade); arginine (commercially available, daily chemical grade).

EXAMPLE 1 preparation of jellyfish collagen solution

Jellyfish (Rhopilema esculentum, jellyfish) is purchased from the aquatic market. 10kg of salted jellyfish was cut into small pieces, washed several times with tap water, and then soaked in pure water for several hours until the salinity was lower than 0.01. 1g of delipase (from Shanghai, ind.) was added, and after soaking at 25℃for 4 hours, the delipase was washed off. The following steps are all carried out below 20 ℃. After homogenization of the material by adding 15L of 0.5mol/L citric acid, the material was transferred to a stirred tank, and enzyme digestion was carried out by adding 10mg/g pepsin (purchased from Shanghai Biotechnology) solution, and the digestion was carried out by slow stirring at 16℃for 12 hours. After digestion, undigested tissue pieces were separated by centrifugation at 17,000g for 30 minutes, the separated supernatant was neutralized to pH7.0 with sodium hydroxide, and the collagen gel pellet was collected by centrifugation at 17,000g for 20 minutes. Then dissolving collagen with 0.05mol/L citric acid with 8 times of precipitation volume, regulating the supernatant to be neutral, and continuously centrifuging to collect gel precipitate. The steps from dissolution of citric acid to centrifugation of gel precipitate were repeated 5 times in the first group, and 6 times in the second group. And after the completion, putting the collagen sponge into a freeze dryer for freeze drying, and preparing the collagen sponge.

Preparation of jellyfish collagen solution

Respectively weighing two groups of collagen sponges, wherein each group comprises three groups of 1 gram of collagen sponges, respectively adding citric acid to adjust the pH value to 3.5 of 50% hydroxyethyl urea solution, and stirring the 65% hydroxyethyl urea solution with the pH value of 4.3 and the 75% hydroxyethyl urea solution with the pH value of 4.5 to 100ml, after the two groups of collagen sponges are fully dissolved, placing the two groups of collagen sponges in a refrigerator at 4 ℃ for storage, observing after 7 days, wherein the first three groups of collagen sponges are turbid to different degrees and cannot be stably stored for a long time; the second set of triplicate still exhibited a uniform transparent state. And selecting a second group of second jellyfish collagen solution (with pH value of 4.3,65% dissolved by hydroxyethyl urea) as a mask manufacturing raw material for standby.

Example 2 mask A was prepared

In the first step, a sufficient amount of deionized water is heated to 80 ℃ for use.

Secondly, 6g of xanthan gum, 100g of glycerin, 50g of butanediol, 1g of sodium hyaluronate and 1.5g of beta-glucan are initially and uniformly mixed in a beaker by a spoon, 600g of preheated ionized water is added, and the mixture is transferred to a constant-temperature heating table for heat preservation and stirring until all materials are dissolved and uniformly dispersed.

Thirdly, soaking the materials in cold water, stirring and cooling to 60 ℃, continuously transferring to a constant temperature heating table for heat preservation and stirring, weighing 30g of water-soluble jojoba grease, 5g of aloe vera extract, 30g of sodium polyglutamate, 30g of epigallocatechin gallate glucoside, 1.5g of Ningxia wolfberry extract, 50g of lentinus edodes mycelium extract, 30g of peach resin extract and 2g of sodium chloride, adding the materials into the beaker, stirring until all materials are fully dissolved and uniformly dispersed, and finishing the preparation of the basic mask liquid.

Fourth, soaking the beaker in cold water, stirring and cooling to 45 ℃. 80g of the jellyfish collagen solution prepared in example 1 and 30g of the fibronectin solution were added and stirred sufficiently until they were uniformly dispersed. Deionized water pre-cooled to 50 ℃ or below was added to the beaker and the total weight of the material was made up to 1000g.

And fifthly, stopping stirring, sampling and checking, and filling the mask liquid into a mask bag containing seaweed membrane cloth after the mask liquid is checked to be qualified, wherein the filling amount is 27 g/bag, sealing, and finishing the preparation of the mask A.

Example 3 mask B

First, a sufficient amount of deionized water was heated to 85 ℃ for use.

Then, 4g of xanthan gum, 70g of glycerin, 40g of butanediol, 0.8g of sodium hyaluronate and 1g of beta-glucan are weighed and initially mixed uniformly in a beaker by a spoon, 600g of preheated ionized water is added, and the mixture is transferred to a constant temperature heating table for heat preservation and stirring until all materials are dissolved and uniformly dispersed.

Thirdly, soaking the materials in cold water, stirring and cooling to 45 ℃, continuously transferring to a constant temperature heating table for heat preservation and stirring, weighing 15g of water-soluble jojoba grease, 3g of aloe vera extract, 20g of sodium polyglutamate, 15g of epigallocatechin gallate, 0.8g of Ningxia wolfberry extract, 30g of lentinus edodes mycelium extract, 20g of peach resin extract and 0.8g of sodium chloride, adding the materials into the beaker, stirring until all materials are fully dissolved and uniformly dispersed, and finishing the preparation of the basic mask liquid.

Fourth, soaking the materials in cold water, stirring and cooling to 45 ℃, adding 50g of jellyfish collagen solution and 10g of fibronectin solution, and fully stirring until the materials are uniformly dispersed. Deionized water pre-cooled to 50 ℃ or below was added to the beaker and the total weight of the material was made up to 1000g.

And fifthly, stopping stirring, sampling and checking, and filling the mask liquid into a mask bag containing seaweed membrane cloth after the mask liquid is checked to be qualified, wherein the filling amount is 27 g/bag, sealing, and finishing the preparation of the mask B.

Example 4 mask C

First, a sufficient amount of deionized water was heated to 85 ℃ for use.

Then weighing 2g of xanthan gum, 1g of cellulose, 50g of glycerin, 30g of butanediol, 0.5g of sodium hyaluronate and 0.3g of beta-glucan, mixing uniformly in a beaker by a spoon, adding 600g of preheated ionized water, transferring to a constant-temperature heating table for heat preservation and stirring until all materials are dissolved and dispersed uniformly.

Thirdly, soaking the materials in cold water, stirring and cooling to 60 ℃, continuously transferring to a constant temperature heating table for heat preservation and stirring, weighing 10g of water-soluble jojoba grease, 2g of aloe vera extract, 10g of sodium polyglutamate, 10g of epigallocatechin gallate glucoside, 0.5g of Ningxia wolfberry extract, 20g of lentinus edodes mycelium extract, 10g of peach resin extract and 0.5g of sodium chloride, adding the materials into the beaker, stirring until all materials are fully dissolved and uniformly dispersed, and finishing the preparation of the basic mask liquid.

Fourth, soaking the materials in cold water, stirring and cooling to 45 ℃, adding 30g of jellyfish collagen solution and 5g of fibronectin solution, and fully stirring until the materials are uniformly dispersed. Deionized water pre-cooled to 50 ℃ or below was added to the beaker and the total weight of the material was made up to 1000g.

And fifthly, stopping stirring, sampling and checking, and filling the mask liquid into a mask bag containing seaweed membrane cloth after the mask liquid is checked to be qualified, wherein the filling amount is 27 g/bag, sealing, and finishing the production of the mask C.

Example 5 mask D

In the first step, a sufficient amount of deionized water is heated to 80 ℃ for use.

Secondly, 6g of xanthan gum, 100g of glycerin, 50g of butanediol, 1g of sodium hyaluronate and 1.5g of beta-glucan are initially and uniformly mixed in a beaker by a spoon, 600g of preheated ionized water is added, and the mixture is transferred to a constant-temperature heating table for heat preservation and stirring until all materials are dissolved and uniformly dispersed.

Thirdly, soaking the materials in cold water, stirring and cooling to 60 ℃, continuously transferring to a constant temperature heating table for heat preservation and stirring, weighing 30g of water-soluble jojoba grease, 5g of aloe vera extract, 30g of sodium polyglutamate, 30g of epigallocatechin gallate glucoside, 1.5g of Ningxia wolfberry extract, 50g of lentinus edodes mycelium extract, 30g of peach resin extract and 2g of sodium chloride, adding the materials into the beaker, stirring until all materials are fully dissolved and uniformly dispersed, and finishing the preparation of the basic mask liquid.

Fourth, soaking the beaker in cold water, stirring and cooling to 45 ℃. 30g of fibronectin solution was added and stirred well until it was dispersed well. Deionized water pre-cooled to 50 ℃ or below was added to the beaker and the total weight of the material was made up to 1000g.

And fifthly, stopping stirring, sampling and checking, and filling the mask liquid into a mask bag containing seaweed membrane cloth after the mask liquid is checked to be qualified, wherein the filling amount is 27 g/bag, sealing, and finishing the preparation of the mask A.

Example 6 mask E was prepared

In the first step, a sufficient amount of deionized water is heated to 80 ℃ for use.

Secondly, 6g of xanthan gum, 100g of glycerin, 50g of butanediol, 1g of sodium hyaluronate and 1.5g of beta-glucan are initially and uniformly mixed in a beaker by a spoon, 600g of preheated ionized water is added, and the mixture is transferred to a constant-temperature heating table for heat preservation and stirring until all materials are dissolved and uniformly dispersed.

Thirdly, soaking the materials in cold water, stirring and cooling to 60 ℃, continuously transferring to a constant temperature heating table for heat preservation and stirring, weighing 30g of water-soluble jojoba grease, 5g of aloe vera extract, 30g of sodium polyglutamate, 30g of epigallocatechin gallate glucoside, 1.5g of Ningxia wolfberry extract, 50g of lentinus edodes mycelium extract, 30g of peach resin extract and 2g of sodium chloride, adding the materials into the beaker, stirring until all materials are fully dissolved and uniformly dispersed, and finishing the preparation of the basic mask liquid.

Fourth, soaking the beaker in cold water, stirring and cooling to 45 ℃. 80g of the jellyfish collagen solution prepared in example 1 was added and stirred well until it was dispersed uniformly. Deionized water pre-cooled to 50 ℃ or below was added to the beaker and the total weight of the material was made up to 1000g.

And fifthly, stopping stirring, sampling and checking, and filling the mask liquid into a mask bag containing seaweed membrane cloth after the mask liquid is checked to be qualified, wherein the filling amount is 27 g/bag, sealing, and finishing the preparation of the mask A.

Example 7 mask F was prepared

In the first step, a sufficient amount of deionized water is heated to 80 ℃ for use.

Secondly, 6g of xanthan gum, 100g of glycerin, 50g of butanediol, 1g of sodium hyaluronate and 1.5g of beta-glucan are initially and uniformly mixed in a beaker by a spoon, 600g of preheated ionized water is added, and the mixture is transferred to a constant-temperature heating table for heat preservation and stirring until all materials are dissolved and uniformly dispersed.

Thirdly, soaking the materials in cold water, stirring and cooling to 60 ℃, continuously transferring to a constant temperature heating table for heat preservation and stirring, weighing 30g of water-soluble jojoba grease, 5g of aloe vera extract, 30g of sodium polyglutamate, 30g of epigallocatechin gallate glucoside, 1.5g of Ningxia wolfberry extract, 50g of lentinus edodes mycelium extract, 30g of peach resin extract and 2g of sodium chloride, adding the materials into the beaker, stirring until all materials are fully dissolved and uniformly dispersed, and finishing the preparation of the basic mask liquid.

Fourth, soaking the beaker in cold water, stirring and cooling to 45 ℃. Deionized water pre-cooled to 50 ℃ or below was added to the beaker and the total weight of the material was made up to 1000g.

And fifthly, stopping stirring, sampling and checking, and filling the mask liquid into a mask bag containing seaweed membrane cloth after the mask liquid is checked to be qualified, wherein the filling amount is 27 g/bag, sealing, and finishing the preparation of the mask A.

The mask formulations prepared in examples 2-7 are shown in table 1. The formula of the mask can be prepared according to the following proportion.

Table 1 mask formulations prepared in example 2-example 7

Example 8 mask lotion moisturizing experiment

10 volunteers were recruited, the whole arm below the right hand elbow was washed with hand wash, dried in the air and waited in a skin test room (room temperature 25 ℃).

The wrist is 6cm upwards, 7 square areas (left 4 and right 3) with 2.5 x 2.5cm are taken, each square area is 1cm apart, the edges of the square areas are drawn by marking strokes, marks 1-7,1 are blank groups, and the rest are experimental groups. Skin moisture tester (fitting probe) with German CK MPA 6CM 825) the skin in the 7 marked areas after washing and airing was subjected to moisture test, denoted T0. Each region was sampled 4 times and then averaged.

The facial mask A, B, C, D, E, F is taken out, the bag is cut off, and facial mask liquid is squeezed into a beaker. And respectively taking 0.5ml of each mask liquid by using a dropper, and uniformly coating the mask liquid corresponding to 2-7 areas on the arms of each person.

After the smeared areas on the arms of the persons are dried, the moisture content of the areas 1-7 is marked as T1, then the moisture content of the 7 areas on the arms of the volunteers is measured at the time points of 30min,1h,2h,4h and 6h respectively and marked as T2, T3, T4, T5 and T6 respectively, and the results are collated into the figure 1. The difference between the moisturizing effect and the duration of the mask A, B is not large, the effect of the mask C is not good enough compared with the effect of the mask C, but the moisturizing effect of the mask C and the mask C is obvious compared with the moisturizing effect of the mask D, E, F, and the moisture content of the skin after 4 hours (T5) is still higher than that of a blank group. While observing the data of mask D, E, F, it is readily seen that even if other components were the same as mask a, with or without the addition of either the fibronectin solution or the jellyfish collagen solution, there was a gap at time T4 compared to the worst of mask a, mask B, and mask C. The fibronectin solution and jellyfish collagen solution are compounded to help promote the moisturizing effect of the mask. The mask B is preferably selected according to the combination of the effect and the manufacturing cost.

Example 9 mask wrinkle removal experiment

96 volunteers were recruited, between ages 25-40, with skin being dry skin or with wrinkles visible to the naked eye. Volunteers were divided into two groups, 48 at ages 25-30 and 31-40 each, into two large A, B groups; A. and 1-6 groups of 8 persons are respectively arranged among the groups B. In this age group, many wrinkles are generated on the dry skin surface because the skin care is not emphasized, the barrier is unstable, the skin is deficient in water, nutrition is lost, etc. (especially in the age group A), and the wrinkles are not fixed. Before the experiment starts, all people clean the face with the facial cleanser, and after the face is cleaned, the residual water stains on the face are firstly and slightly wiped off with a dry towel. All volunteers were transferred to a skin test room, air-conditioned at room temperature of 25 ℃ for 5min and left to sit for 5min to dry the towel without wiping off moisture.

After the moisture was dried, each person was feature recorded by taking a picture of each person's face using a U.S. VISIA skin detector in sequence, and recording the score value determination of VISIA for wrinkles (higher score value demonstrates higher score of this item, better result) was marked as D0. After facial features are recorded, 10 pieces of facial masks are dispensed to each volunteer, and 1-6 pieces of A, B respectively correspond to the dispensed facial masks A-F. Then, people return to use a mask after bath every night and check in by punching cards to prevent missing application. On the 6 th day, volunteers were again collected uniformly for performing the skin test of the ViSIA, the face was washed in advance as in the first test before the test and rested in the skin test room for 5min, and the wrinkle percentage value was recorded as D1 after the test. The next day after the 10 th mask was applied, skin detection was continued to record the percentage of wrinkles, labeled D2.

And processing experimental data, subtracting D0 from D1, subtracting D1 from D2 to obtain a percentage value difference a value and a percentage value b value of two stages, and subtracting D0 from D2 to obtain a percentage value difference c value before and after the experiment. Group a maximum c occurs at A1 of 61 (see fig. 2) and group B maximum c occurs at B1 of 46 (see fig. 3). The percentage differences for each group of volunteers were averaged to retain a fraction (see table 2).

As is easy to see from the analysis results, the A, B group performs intra-group comparison, the data of the groups 1, 2 and 3 are the best, wherein the data of the group 1 are the best data in the group, the best effect of the mask a is proved, the data of the group 2 is not different from the final data of the group 1, and the mask B is still the best choice in consideration of the comprehensive effect and the manufacturing cost. According to the analysis of A, B arbitrary large group data, even if other components are consistent with the mask A, the effect of the mask D-F is inferior to that of the worst mask C in A, B, C, which indicates that the combination of the fibronectin solution and the jellyfish collagen solution is beneficial to obviously improving the wrinkle removing effect of the mask.

TABLE 2 wrinkle removal test data

Example 10 mask repair skin Barrier test

The red-colored photograph of one of the ViSIA skin test items can detect problems associated with subcutaneous blood vessels, typically associated with acne, skin sensitivity, telangiectasia, etc., and a lower detection percentage value reflects a thinner skin and a more sensitive skin, typically used to detect damage to the skin barrier.

120 volunteers with sensitive muscles were recruited, and the groups A, B, and A, B were each divided into 1-6 groups, respectively, 20-30 years old 60, 30-40 years old 60, and 6 groups. Before the experiment starts, all people clean the face with the facial cleanser, and after the face is cleaned, the residual water stains on the face are firstly and slightly wiped off with a dry towel. All volunteers were transferred to a skin test room, air-conditioned at room temperature of 25 ℃ for 5min and left to sit for 5min to dry the towel without wiping off moisture.

After the moisture is dried, each person sequentially uses a American VISIA skin detector to take a picture of the face of each person for characteristic recording, and the percentage value of the VISIA to the red area is recorded for judgment. After facial features are recorded, 10 pieces of facial masks are issued to each volunteer, and 1-6 groups of A, B groups respectively correspond to the facial masks A-F. Then, people return to use a mask after bath every night and check in by punching cards to prevent missing application. When the 10 th mask was used, the skin test was continued for recording the red color percentage value.

The average after the second skin test red zone results were recorded was rounded off and recorded as a table (see table 3,0 means the average was less than 1 or negative). The results were recorded and found that the two groups of volunteers were most significantly elevated in terms of percentage of facial red area over 10 days of use, using the mask a (groups A1 and B1), with group A1 having an elevation maximum of 35 (see fig. 4) and group B1 having an elevation maximum of 32 (see fig. 5). A. The small component values of the facial mask B, C are obviously improved when the two groups B are compared in a group, and the facial mask B is the best choice in consideration of comprehensive effects and manufacturing cost. From the comparison of the data of the use of mask D in the A, B large group and the use of mask a in the group, it is easy to see that mask D with only the addition of fibronectin solution also has a significant effect on skin barrier repair; the combination of the group data using mask E and mask F shows that the addition of jellyfish collagen solution alone has no obvious effect on skin barrier repair; however, the combination of the data from the panel A, D, E, F group shows that the combination of fibronectin and jellyfish collagen solutions greatly increases the skin barrier repair effect.

TABLE 3 red differentiation value improvement cases

The above description of the specific embodiments of the present invention has been given by way of example only, and the present invention is not limited to the above described specific embodiments. Any equivalent modifications and substitutions for this invention will occur to those skilled in the art, and are intended to be within the scope of this invention. Accordingly, equivalent changes and modifications are intended to be included within the scope of the present invention without departing from the spirit and scope thereof.

Sequence listing

<110> Meier's (Shenzhen) Biotechnology Co., ltd

<120> a mask based on jellyfish collagen solution and method for preparing the same

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<141> 2021-12-30

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Pro Gly Thr Glu Tyr Val Val Ser Val Ser Ser Val Tyr Glu Gln His

165 170 175

Glu Ser Thr Pro Leu Arg Gly Arg Gln Lys Thr Gly Leu Asp Ser Pro

180 185 190

Thr Gly Ile Asp Phe Ser Asp Ile Thr Ala Asn Ser Phe Thr Val His

195 200 205

Trp Ile Ala Pro Arg Ala Thr Ile Thr Gly Tyr Arg Ile Arg His His

210 215 220

Pro Glu His Phe Ser Gly Arg Pro Arg Glu Asp Arg Val Pro His Ser

225 230 235 240

Arg Asn Ser Ile Thr Leu Thr Asn Leu Thr Pro Gly Thr Glu Tyr Val

245 250 255

Val Ser Ile Val Ala Leu Asn Gly Arg Glu Glu Ser Pro Leu Leu Ile

260 265 270

Gly Gln Gln Ser Thr Val Ser Asp Val Pro Arg Asp Leu Glu Val Val

275 280 285

Ala Ala Thr Pro Thr Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val

290 295 300

Thr Val Arg Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser

305 310 315 320

Pro Val Gln Glu Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile

325 330 335

Ser Gly Leu Lys Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Val

340 345 350

Thr Gly Arg Gly Asp Ser Pro Ala Ser Ser Lys Pro Ile Ser Ile Asn

355 360 365

Tyr Arg Thr

370

Claims (8)

1. The mask based on jellyfish collagen is characterized by comprising mask liquid and mask cloth, wherein the mask liquid comprises the following components in parts by weight:

11.58-25.25 parts of basic mask liquid;

3-8 parts of jellyfish collagen solution;

0.5-3 parts of fibronectin solution;

58.3-81.82 parts of deionized water;

the basic mask liquid comprises the following components in parts by weight:

0.3-0.6 parts of xanthan gum;

5-10 parts of glycerol;

3-5 parts of butanediol;

0.05-0.1 part of sodium hyaluronate;

0.03 to 0.15 of beta-glucan;

1-3 parts of water-soluble jojoba grease;

0.2-0.5 parts of aloe vera extract;

1-3 parts of sodium polyglutamate;

1-3 parts of epigallocatechin gallate;

0.05-0.15 part of Ningxia wolfberry extract;

2-5 parts of lentinus edodes mycelium extract;

1-3 parts of peach resin extract;

sodium chloride 0.05-0.2 parts.

2. The jellyfish collagen-based mask of claim 1, wherein the mask cloth is a seaweed film.

3. The jellyfish collagen-based mask of claim 1, wherein the fibronectin in the fibronectin solution has an amino acid sequence shown in SEQ ID No. 1.

4. The jellyfish collagen-based mask of claim 3, wherein the concentration of fibronectin in the fibronectin solution is 500ppm.

5. The jellyfish collagen-based mask of claim 1, wherein the jellyfish collagen solution is prepared by the following preparation method:

step one, preparing collagen sponge: extracting collagen sponge from jellyfish;

and step two, dissolving the collagen sponge into a 50% -75% hydroxyethyl urea solution with the pH value adjusted to 3.5-4.5 by citric acid.

6. The jellyfish collagen-based mask of claim 5, wherein the collagen sponge of the first step is prepared by:

A. desalting jellyfish;

B. lipid removal substances: adding a delipase, soaking, and washing off the delipase;

C. c, adding citric acid, homogenizing the material subjected to degreasing in the step B, transferring, and then adding pepsin solution for enzyme digestion;

D. after digestion, centrifugally separating undigested tissue blocks, neutralizing the separated supernatant with alkali liquor to be neutral, and centrifugally collecting collagen gel precipitate;

E. dissolving collagen with citric acid, regulating the supernatant to neutrality, and centrifuging to collect gel precipitate; repeating the steps for more than or equal to N times, wherein N is a natural number; then, the collagen sponge is put into a freeze dryer for freeze drying, and the collagen sponge is prepared.

7. The jellyfish collagen-based mask of claim 6, wherein the step of dissolving the citric acid to centrifugally collecting the gel precipitate is repeated 6 times.

8. A method of preparing a mask according to any one of claims 1 to 6, comprising the steps of:

in a first step, the following materials are prepared in parts by weight according to claim 1: xanthan gum, glycerin, butylene glycol, sodium hyaluronate, beta-glucan, water-soluble jojoba ester, aloe vera extract, sodium polyglutamate, epigallocatechin gallate, lycium barbarum extract, shiitake mushroom hypha extract, peach resin extract, sodium chloride, jellyfish collagen solution and fibronectin solution;

step two, weighing xanthan gum, glycerin, butanediol, sodium hyaluronate and beta-glucan, mixing uniformly, adding deionized water preheated to 80-85 ℃, stirring until all materials are dissolved and dispersed uniformly;

secondly, cooling to below 60 ℃, adding water-soluble jojoba, aloe vera extract, sodium polyglutamate, ningxia wolfberry extract, epigallocatechin gallate, lentinus edodes mycelium extract, peach resin extract and sodium chloride, stirring until all materials are dissolved and dispersed uniformly;

thirdly, cooling to below 45 ℃, adding the jellyfish collagen solution and the fibronectin solution, and fully stirring until the jellyfish collagen solution and the fibronectin solution are uniformly dispersed, thus finishing the preparation of the mask liquid;

fourth, sampling and detecting indexes such as microorganisms and heavy metals, transferring to a mask filling machine to fill a mask bag containing seaweed membrane cloth after the indexes are qualified, and finishing the manufacturing of the mask after sealing.