CN116584201B - Method for accelerating germination of luffa seeds with edges and improving seedling rate - Google Patents
Method for accelerating germination of luffa seeds with edges and improving seedling rate Download PDFInfo
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- 230000035784 germination Effects 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 30
- 235000003956 Luffa Nutrition 0.000 title claims description 21
- 244000050983 Luffa operculata Species 0.000 title 1
- 241000219122 Cucurbita Species 0.000 claims abstract description 60
- 235000009852 Cucurbita pepo Nutrition 0.000 claims abstract description 60
- 238000002791 soaking Methods 0.000 claims abstract description 44
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 35
- 239000011259 mixed solution Substances 0.000 claims abstract description 29
- 238000011282 treatment Methods 0.000 claims abstract description 29
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 20
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 244000280244 Luffa acutangula Species 0.000 claims abstract description 11
- 235000018780 Luffa acutangula Nutrition 0.000 claims abstract description 10
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 3
- 241000219138 Luffa Species 0.000 claims description 20
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 12
- 229920000742 Cotton Polymers 0.000 claims description 10
- 239000004744 fabric Substances 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 8
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract description 8
- 230000018109 developmental process Effects 0.000 abstract description 7
- 230000012010 growth Effects 0.000 abstract description 7
- 230000005059 dormancy Effects 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000007226 seed germination Effects 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 238000010521 absorption reaction Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 239000007864 aqueous solution Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000001133 acceleration Effects 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 4
- 210000001161 mammalian embryo Anatomy 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 208000019300 CLIPPERS Diseases 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 208000021930 chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids Diseases 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 235000014347 soups Nutrition 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 229930191978 Gibberellin Natural products 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 208000012868 Overgrowth Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 2
- 239000003448 gibberellin Substances 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000219104 Cucurbitaceae Species 0.000 description 1
- 235000009814 Luffa aegyptiaca Nutrition 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to the technical field of planting of towel gourd with arris, in particular to a method for accelerating germination of towel gourd seeds with arris and improving seedling rate. The method comprises the following steps: soaking the towel gourd seeds with the arris fruits in the mixed solution, and then carrying out germination accelerating treatment; the mixed solution is a mixed solution of clear water, hydrogen peroxide and monopotassium phosphate. The method for accelerating germination of the ribbed luffa seeds and improving the emergence rate provided by the invention can break the dormancy of the seeds after treatment, obviously shorten the germination accelerating time of the seeds, improve the germination rate and the germination vigor of the ribbed luffa seeds, and promote the growth and development of the ribbed luffa seedlings, and is convenient and simple in method and simple in operation. The towel gourd seeds treated by the method have strong vitality and vigorous seed vitality, can promote the rapid germination and emergence of the seeds, ensure that the emergence of the seeds is tidy and good in consistency, are beneficial to standardized and mechanized production of towel gourd, provide technical support for large-scale and high-quality development of towel gourd, and promote industrialized development of towel gourd.
Description
Technical Field
The invention relates to the technical field of planting of towel gourd with arris, in particular to a method for accelerating germination of towel gourd seeds with arris and improving seedling rate.
Background
The towel gourd with the edge is an annual climbing herb plant of the towel gourd genus of the cucurbitaceae family, and is popular with people because of the rich quality, the crisp taste. In recent years, various application researches such as medicinal components, beverage processes, living goods and the like are carried out on the towel gourd with the edge, and various towel gourd products are also developed, so that the towel gourd with the edge has wider application range and higher economic value. However, in the aspect of planting the towel gourd with the arris, the germination accelerating time of seeds is long, the germination rate is relatively low, the germination potential is not good enough, so that the towel gourd with the arris has the advantages of untidy emergence, weak seedlings and easy overgrowth, the planting workload of the towel gourd with the arris is increased, and the planting cost is greatly increased.
Therefore, the seed treatment method for accelerating germination of the ribbed towel gourd and improving the seedling rate can improve the planting efficiency of the ribbed towel gourd, reduce the workload, reduce the management cost of the planting process, accelerate the seedling emergence time and the breeding process, and has great significance for the technical field of ribbed towel gourd planting.
Disclosure of Invention
The invention aims to provide a method for accelerating germination of luffa seeds with edges and improving seedling rate so as to solve the problems in the prior art.
In order to achieve the above object, the present invention provides the following solutions:
the invention provides a method for accelerating germination of luffa seeds with edges and improving seedling rate, which comprises the following steps: soaking the towel gourd seeds with the arris fruits in the mixed solution, and then carrying out germination accelerating treatment;
the mixed solution is a mixed solution of hydrogen peroxide and potassium dihydrogen phosphate.
Further, the mass concentration of hydrogen peroxide in the mixed solution is 0.04-0.06%, and the volume concentration of potassium dihydrogen phosphate is 0.09-0.12%.
The hydrogen peroxide treatment with proper concentration can promote the germination of seeds, improve the germination rate of seeds and shorten the breeding period. Excessive concentration can cause the towel gourd seedlings with the arris to grow excessively and have high feet, even cotyledons to curl, and the growth and development are abnormal; the concentration is low, and the seed germination accelerating agent has no promotion effect on seed germination. The potassium dihydrogen phosphate solution with proper concentration can soak the seeds to make the seeds germinate, emerge and align. Too high concentration of potassium dihydrogen phosphate can cause fertilizer damage, seedling burning and root burning, and too low concentration has little influence on promoting plant growth and development. The experimental study shows that after the hydrogen peroxide with the mass concentration of 30% is diluted by 500-700 times, the seed soaking and germination accelerating effects are obvious for accelerating the seed germination. Meanwhile, after the monopotassium phosphate is diluted by 900-1100 times by clear water, seed soaking and germination accelerating can also promote seed germination, and the plant growth promoting agent has certain functions of coarse root and strong seedling. Therefore, the invention limits the mass concentration of hydrogen peroxide in the mixed solution to 0.04-0.06%, and the volume concentration of potassium dihydrogen phosphate to 0.09-0.12%.
The hydrogen peroxide can promote germination of the luffa seeds with the arris, accelerate germination time and improve germination rate, and the potassium dihydrogen phosphate is used for soaking seeds, so that nutrition accumulation can be provided for seed germination, and the kinetic energy of nutrient substances is provided in the seed germination process, so that the luffa seeds with the arris has a good effect on cultivating robust seedlings. Therefore, the hydrogen peroxide and the monopotassium phosphate are mixed and compounded, so that the germination of the luffa seeds with the edges can be accelerated, the germination rate is improved, the breeding period is shortened, and meanwhile, enough nutrition components can be provided in the seed germination process, so that the effect of root cultivation and seedling strengthening is achieved.
Further, the step of breaking the shell of the towel gourd seeds before soaking the towel gourd seeds in the mixed solution is further included.
Further, the depth of the crust breaking treatment is 1.8-2.2 mm. The shell breaking treatment does not damage the embryo.
Further, the soaking is specifically as follows: firstly, soaking for 15-25min at 53-57 ℃, and then soaking for 2-4h in the environment of 30-32 ℃.
The reason of soaking at 53-57 ℃ is mainly that seed disinfection is achieved by soaking seeds in warm soup, moisture is fully absorbed, pathogenic bacteria hidden on the surfaces and inside of the seeds are killed, and plant diseases are reduced. Under normal conditions, the seed soaking temperature of the warm soup is 50-55 ℃, the effects of accelerating absorption and swelling of seeds during soaking, promoting the metabolic process before germination, having good germination accelerating effect, and also performing seed disinfection and sterilization. The seed leather of the luffa seeds is smooth and hard, so that the water is not easy to absorb in the seed soaking process. Therefore, the temperature range of the heat preservation soaking can be slightly larger, and meanwhile, the soaking effect is optimal at 53-57 ℃ through experiments. Too high a temperature can directly scald or even burn the seeds, so that the seeds can be directly lost; the temperature is too low, the water absorption of the seeds is insufficient, and in addition, the functions of seed disinfection, sterilization and even disinsection cannot be achieved.
The soaking time is 15-25min, and can achieve the aim of fully sterilizing seeds due to combination with temperature, and the proper moisture absorption in the soaking process can also promote seed germination. When the soaking time is too long, water can be absorbed too much, and the phenomenon of overgrowth and even inactivation after emergence of seedlings can occur; too short soaking time and insufficient water absorption can lead to low germination rate and untidy emergence of seedlings.
Soaking at 30-32deg.C, which is suitable for germination and germination acceleration of seeds of Luffa acutanga at 30-32deg.C, and excessive temperature can cause excessive growth of seeds after emergence of seedlings or even loss of vigor of seeds; the temperature is too low, as in normal temperature seed soaking, the soaking time is prolonged, or the water absorption is insufficient. The soaking time is 2-4h, and the normal seed soaking time is 5-6h, and the water absorption can be accelerated after the seed crust breaking treatment, so that the soaking time is reduced to 2-4h. Too long time, the seeds are inactivated, the germination rate is not high, the time is too short, the soaking is insufficient, the moisture absorption is insufficient, and the germination acceleration is not ideal.
Further, the soaking step further comprises the step of cleaning and sucking the surface moisture of the towel gourd seeds with the edges.
Further, the germination accelerating treatment specifically comprises the following steps: wrapping with wet cotton cloth, and placing in a dark environment with the temperature of 30-32 ℃ and the humidity of 70-80% for 14-16 h.
The germination accelerating agent has the optimal germination accelerating effect on the seeds of the luffa acutangula under the environment with the temperature of 30-32 ℃ and the humidity of 70-80%, and the germination accelerating agent can accelerate germination in the period of 14-16 hours, so that the seeds are just exposed to white, the germination point is not too long, and the germination accelerating agent is just suitable for sowing.
The invention discloses the following technical effects:
the method for accelerating germination of the ribbed luffa seeds and improving the emergence rate provided by the invention can break the dormancy of the seeds after treatment, obviously shorten the germination accelerating time of the seeds, improve the germination rate and the germination vigor of the ribbed luffa seeds, and promote the growth and development of the ribbed luffa seedlings, and is convenient and simple in method and simple in operation. The towel gourd seeds treated by the method have strong vitality and vigorous seed vitality, can promote the rapid germination and emergence of the seeds, ensure that the emergence of the seeds is tidy and good in consistency, the germination vigor is as high as 87%, the germination rate is more than 97%, the growth vigor is uniform, are favorable for standardized and large-scale production of towel gourd, and the healthy growth of plants after emergence can effectively improve the emergence rate, and the emergence rate is as high as 96%, thereby providing technical support for standardized and high-quality development of the towel gourd and promoting industrialized development of the towel gourd.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the seed shell breaking treatment of the luffa seeds with edges according to the present invention.
FIG. 2 is a schematic diagram of a seedling population of luffa seeds with edges in example 2.
FIG. 3 is a schematic diagram showing the growth of seedlings of luffa with edges in example 2 and comparative example 1.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
In the present invention, "%" indicates mass percent unless otherwise specified.
A schematic diagram of the loofah seed shell breaking treatment in the invention is shown in figure 1.
Example 1
Step 1, performing shell breaking treatment on the towel gourd seeds with the edges: a small opening is cut from the navel of the luffa seed by using nail clippers (or other tools), the seed embryo is not damaged by the opening, and the seed crust breaking treatment depth is 1.8mm.
And 2, soaking the shell-broken towel gourd seeds in a mixed solution (the mixed solution is 30% hydrogen peroxide aqueous solution with the volume multiple of 500 diluted by clear water, and simultaneously is 900% monopotassium phosphate aqueous solution with the volume multiple, namely, the mass concentration of hydrogen peroxide in the mixed solution is 0.060%, the volume concentration of monopotassium phosphate is 0.110%) at 53 ℃ for 15min, soaking the towel gourd seeds in a light incubator at 30 ℃ for 2h, taking out the towel gourd seeds, soaking and cleaning the towel gourd seeds twice by tap water, spin-drying the towel gourd seeds, pressing and absorbing the surface moisture of the towel gourd seeds by adopting dry cotton cloth, wrapping the towel gourd seeds by adopting wet cotton cloth, and placing the towel gourd seeds in the light incubator for 14h under the constant temperature and humidity of 30 ℃ and 70%.
Example 2
Step 1, performing shell breaking treatment on the towel gourd seeds with the edges: a small opening is cut from the navel of the luffa seed by using nail clippers (or other tools), the seed embryo is not damaged by the opening, and the seed crust breaking treatment depth is 2.0mm.
And 2, soaking the shell-broken towel gourd seeds in a mixed solution (the mixed solution is 30% hydrogen peroxide aqueous solution with the volume multiple of 600 diluted by clear water, and simultaneously is 1000% potassium dihydrogen phosphate aqueous solution with the volume multiple of 1000, namely, the mass concentration of hydrogen peroxide in the mixed solution is 0.0500%, the volume concentration of potassium dihydrogen phosphate is 0.1000%) at 55 ℃ for 20min, soaking the towel gourd seeds in a 31 ℃ illumination incubator for 3h, taking out the towel gourd seeds, soaking and cleaning the towel gourd seeds twice by tap water, spin-drying the towel gourd seeds, pressing and absorbing the water on the surfaces of the towel gourd seeds by adopting dry cotton cloth, wrapping the towel gourd seeds by adopting wet cotton cloth, and placing the towel gourd seeds in the illumination incubator for 15h under the constant temperature and humidity of 31 ℃.
Example 3
Step 1, performing shell breaking treatment on the towel gourd seeds with the edges: a small opening is cut from the navel of the luffa seed by using nail clippers (or other tools), the seed embryo is not damaged by the opening, and the seed crust breaking treatment depth is 2.2mm.
And 2, soaking the shell-broken towel gourd seeds in a mixed solution (the mixed solution is 30% hydrogen peroxide aqueous solution with the volume multiple of 700 diluted by clear water, and simultaneously is 1100% monopotassium phosphate aqueous solution with the volume multiple, namely, the mass concentration of hydrogen peroxide in the mixed solution is 0.0429%, the volume concentration of monopotassium phosphate is 0.0910%) at 57 ℃ for 25min, soaking the towel gourd seeds in a 32 ℃ illumination incubator for 4h, taking out the towel gourd seeds, soaking and cleaning the towel gourd seeds twice by tap water, spin-drying the towel gourd seeds, pressing and absorbing the surface moisture of the towel gourd seeds by adopting dry cotton cloth, wrapping the towel gourd seeds by wet cotton cloth, and placing the towel gourd seeds in the illumination incubator for germination accelerating treatment at the constant temperature of 32 ℃ and the humidity of 80% under constant temperature and constant humidity for 16h.
Comparative example 1
The conventional germination accelerating treatment method for the luffa seeds comprises the following steps: taking out the ribbed luffa seeds from the packaging bag (if a plurality of varieties exist, respectively bagging the seeds by sand bags, marking), taking clear water at about 55 ℃ (soaking the seeds in warm soup for about 15 min), soaking the seeds in clear water or warm water for 5-8h, fishing out, spin-drying the moisture in the sand bags, squeezing the seeds by gauze or cotton cloth, sucking the excessive moisture, wrapping the seeds by wet cotton cloth, placing the seeds in an illumination incubator at about 30 ℃ for moisture preservation and germination acceleration, and after the germination acceleration time is 18-26h, sowing the seeds.
Comparative example 2
The only difference from example 2 is that step 1 is omitted.
Comparative example 3
The difference from example 2 is only that in step 2, the addition of hydrogen peroxide in the mixed solution was omitted, i.e., the mixed solution was only a 1000 volume multiple of the aqueous potassium dihydrogen phosphate solution.
Comparative example 4
The difference from example 2 is only that in step 2, the addition of potassium dihydrogen phosphate in the mixed solution was omitted, i.e., the mixed solution was only a 30% aqueous hydrogen peroxide solution diluted with clear water by a volume multiple of 600.
Comparative example 5
The only difference from example 2 is that in step 2, the mixed solution is replaced with a formulation prepared by:
(1) Dissolving gibberellin 0.02g and indoleacetic acid 0.02g with a small amount of ethanol (the ethanol is used for dissolving gibberellin 0.02g and indoleacetic acid 0.02 g);
(2) Respectively weighing 5g of monopotassium phosphate, 1g of copper sulfate, 2g of magnesium sulfate, 3g of sodium chloride, 0.8g of ammonium molybdate, 0.5g of potassium iodide and 1g of ferrous sulfate, and dissolving in 100mL of water;
(3) Pouring the materials (1) and (2) into a measuring cylinder of 1L, and fixing the volume to 1L.
The same batch of luffa seeds was treated with the treatment methods of examples 1-3 and comparative examples 1-5, respectively, each treatment was performed with 4 varieties, summer 6, summer green 5, 22-1, 22-2, respectively. Each treated seed was 500 pieces and repeated three times. Sowing and raising seedlings by using 32-hole seedling raising trays after the treatment, and planting the seedlings subjected to different treatments in the field and cultivating in open field after the seedlings are formed. Other fields are managed conventionally except for different seed treatment methods. And (5) counting germination rate, germination vigor and seedling rate. Germination rate = total normal number of germinated seeds/number of tested seeds x 100% during seed germination (first 7 d); germination potential = total normal number of germinated seeds/number of tested seeds x 100% at initial stage of seed germination test (first 5 d); yield = (number of seedlings +.seeding number) ×100%. The statistical results are shown in Table 1.
TABLE 1
FIG. 2 is a schematic diagram of a seedling population of luffa seeds with edges according to example 2 of the present invention. From fig. 2, it can be seen that after the mixed solution treatment, the towel gourd seedlings are tidy, good in consistency and relatively strong.
FIG. 3 is a schematic diagram showing the growth of seedlings of luffa with edges in example 2 and comparative example 1. From fig. 3, it can be seen that the seedlings of example 2 were larger than those of comparative example 1, the plants were grown relatively faster, and the stems were also relatively larger.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (3)
1. A method for accelerating germination of luffa seeds with edges and improving seedling rate, which is characterized by comprising the following steps: soaking the towel gourd seeds with the arris fruits in the mixed solution, and then carrying out germination accelerating treatment;
the mixed solution is a mixed solution of hydrogen peroxide and potassium dihydrogen phosphate;
the mass concentration of hydrogen peroxide in the mixed solution is 0.04-0.06%, and the volume concentration of potassium dihydrogen phosphate is 0.09-0.12%;
the step of breaking the shells of the towel gourd seeds with the arris is further included before the towel gourd seeds with the arris are soaked in the mixed solution;
the depth of the shell breaking treatment is 1.8-2.2 mm;
the soaking is specifically as follows: firstly, soaking for 15-25min at 53-57 ℃, and then soaking for 2-4h under the illumination condition of 30-32 ℃.
2. The method for accelerating germination and increasing seedling rate of ribbed luffa seeds according to claim 1, further comprising the step of washing and sucking water on the surface of ribbed luffa seeds after soaking.
3. The method for accelerating germination and increasing the seedling rate of the luffa seeds with edges according to claim 1, wherein the germination accelerating treatment is specifically: wrapping with wet cotton cloth, and placing for 14-16h in a dark environment with the temperature of 30-32 ℃ and the humidity of 70-80%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310811568.0A CN116584201B (en) | 2023-07-04 | 2023-07-04 | Method for accelerating germination of luffa seeds with edges and improving seedling rate |
Applications Claiming Priority (1)
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| CN109526984A (en) * | 2018-12-12 | 2019-03-29 | 福建农林大学 | A method of promoting the sprouting of sponge gourd seed and seedling |
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