CN100360008C - Quick method for breeding rosewood - Google Patents
Quick method for breeding rosewood Download PDFInfo
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- CN100360008C CN100360008C CNB2005100189705A CN200510018970A CN100360008C CN 100360008 C CN100360008 C CN 100360008C CN B2005100189705 A CNB2005100189705 A CN B2005100189705A CN 200510018970 A CN200510018970 A CN 200510018970A CN 100360008 C CN100360008 C CN 100360008C
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- rosewood
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- culture medium
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Abstract
The present invention discloses a method for quickly breeding rosewood. A rosewood seed used as an explant germinates on a culture medium for seed germination; substances with different ingredients are added into cultivation based on an improve MS culture medium so that the seed produces clump buds and endlessly proliferates and grows roots. The method comprises the procedures of germination, proliferation, root growth and cultivation. The method of the present invention has the advantages of simple and easy use, convenient operation and high breeding speed.
Description
Technical field
The invention belongs to plant seedling propagation technique field, more specifically relate to a kind of rosewood method of breeding fast.
Technical background
Rosewood belongs to pulse family Ormosia plant.Rosewood is evergreen dungarungas of the four seasons, and is high 5～13 meters.The odd number winglike compound leaf, leaflet 5-9 piece, Long Circle or oval shape fall lanceolar, below close living lark pubescence; The close living lark fine hair of sprout; Raceme, brightly yellowish white, the florescence 6-7 month; The fruit phase 8-9 month, pod is flat, seed redness, thousand kernel weight 540 grams; Winglike compound leaf, the four seasons are evergreen, do not have obviously fallen leaves phenomenon; Dryness is strong, upright, no sprout tillers; Bark is smooth, green; The tree crown taper shape.Rosewood is a Chinese Second Class Key Protected Plant, and timber is orange to bronzing, fine quality, air-dry density 0.75 gram/cm3, and the decorative pattern color and luster is attractive in appearance, for homemade precious name material, can be used as high-grade furniture material; The smooth celadon that is of rosewood trunk epidermis, branches and leaves are luxuriant, are a kind of good novel evergreen afforestation seeds, and low temperature that can be anti--13 ℃ and+40 ℃ high temperature are suitable as the cultivation of street tree and garden; The root of rosewood, branch, leaf can be used as medicine, and the stasis of blood is gone in energy dispelling wind-evil and transforming calculus, detoxifcation.
The modes of reproduction of rosewood is based on seminal propagation.Under field conditions (factors), seed is scattered and is difficult to the nature germination, therefore can't see the seedling of self-sow under the big tree of rosewood, and this is because the kind skin of rosewood has wax coat, has hindered seed suction, germination.At present seeding and seedling raisings that adopt more, if but kind of a skin is not handled prior to seeding, then germination rate after planting is extremely low, generally less than 10%, handles then germination rate and can bring up to about 70% as kind of a skin being broken skin.The natural propagation rate utmost point rate of rosewood, the negligible amounts of rosewood in the open air has been listed in the rare and endangered tree species of national second class protection, and the output of annual seed can't satisfy the demand of existing market.
The method of rosewood seeding and seedling raising is: be the sowing time February to March.Before broadcasting, after removing the epicutile wax layer, seed is placed container, pour 40 ℃ of warm water into, cooling was soaked 24 hours naturally; Soaked 24 o'clock with 40 ℃ of warm water again, wait to broadcast after drying in the shade.The seedbed will use sufficient base manure, and carries out soil disinfection.The strip program request, bar is apart from 20 centimetres.Broadcast back earthing lid grass.After coming up, in time take off grass to May April, weeding in time, loosens the soil and irrigate.Stop the nursery stock fertilising September, to promote nursery stock lignification early.The key of this kind method is every seed kind skin will be peelled off or staved prior to seeding, and only in this way seed could absorb water, germinate, otherwise seed is difficult for suction and causes the germination rate utmost point.Operate pretty troublesomely and each seed will be staved, workload is also very big, need a lot of artificial, so time-consuming, take a lot of work, the production cost height.
Summary of the invention
The object of the present invention is to provide a kind of rosewood method of breeding fast, method is simple, easy to operate, adopts this kind method can breed a large amount of rosewood seedlings fast, with low cost, all can satisfy the demand in market.
In order to achieve the above object, the present invention adopts following technical measures: as explant, make seed sprouting with the rosewood seed under aseptic condition, then downcut the young sprout that grows, insert and make its propagation in the medium, take root into the complete plant of a strain.
In order to enlarge the quantity of rosewood seedling fast, adopt the method for tissue culture, can obtain a large amount of rosewood seedlings fast, to satisfy the demand of market to rosewood, a general seed can breed 1000～2000 seedlings in 1 year.The steps include:
At first be to germinate: will spend the palmitic acid seed to sterilize 8～15 minutes with 0.1% mercuric chloride, with aseptic water washing 4～5 times, the seed that disinfects is inserted in the germination medium for preparing, and this medium only contains agar 3000～5000mg/L, sugar 10000～20000mg/L; Illumination every day 12～14 hours, light intensity 2000～3000Lx, temperature is to cultivate under 24～26 ℃ the condition; Cultivate that seed can germinate after 2～7 days.
Next is a propagation: grow to the high young sprout of 2～5cm after will germinateing and downcut, make young sprout constantly breed in the proliferated culture medium that access prepares and form the bud of growing thickly, this proliferated culture medium comprises the additional composition of the first improvement MS minimal medium and proliferated culture medium, and the first improvement MS minimal medium composition is (mg/L of unit):
The composition of the first improvement MS minimal medium (unit: mg/L):
Nitric acid ammonia (NH
4NO
3) 800～1650
Potassium nitrate (KNO
3) 850～1900
Calcium chloride dihydrate (CaCl
22H
2O) 400～550
Epsom salt (MgSO
47H
2O) 180～370
Potassium dihydrogen phosphate (KH
2PO
4) 80～170
Potassium iodide (KI) 0.83
Boric acid (H
3BO
3) 6.2
Four water manganese sulphate (MnSO
44H
2O) 22.3
White vitriol (ZnSO47H2O) 8.6
Sodium Molybdate Dihydrate (Na2MoO42H2O) 0.25
Cupric sulfate pentahydrate (CuSO45H2O) 0.025
CoCL2 (CoCl26H2O) 0.025
Ferrous sulfate heptahydrate (FeSO47H2O) 27.8
Disodium ethylene diamine tetraacetate (Na
2EDTA2H
2O) 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
The additional composition of proliferated culture medium is 6-benzyl aminopurine (6-Benzyl aminopurine is called for short BA) 0.1～3mg/L, methyl (I-NaphthyIacetic acid is called for short NAA) 0.01～1.0mg/L, agar 3000～5000mg/L, sugar 20000～30000mg/L; Illumination cultivation, temperature are 24～26 ℃, light intensity 2000～3000Lx, and illumination every day 12～14 hours was expanded once numerous in per 30～50 days.
The 3rd is culture of rootage: when breeding to requirement, the young sprout cutting-out that 3～5cm is long inserts the root media for preparing makes the long root of young sprout, this root media comprises the additional composition of the second improvement MS minimal medium and root media, the composition of the second improvement MS minimal medium (unit: mg/L):
NH
4NO
3 550～850
KNO
3 600～1000
CaCl
2·2H
2O 400～600
MgSO
4·7H
2O 100～200
KH
2PO
4 50～90
KI 0.2～0.5
H
3BO
3 2.00～3.0
MnSO
4·4H
2O 7.0～12.0
ZnSO
4·7H
2O 2.5～4.5
Na
2MoO
4·2H
2O 0.08～0.125
CuSO
4·5H
2O 0.008～0.0125
CoCl
2·6H
2O 0.008～0.0125
FeSO
4·7H
2O 9.0～14.0
Na
2·EDTA·2H
2O 12.00～18.00
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
The additional composition of root media is: draw diindyl butyric acid (IBA) 0.1～2mg/L, NAA0.01～1mg/L agar 3000～5000mg/L, sugar 10000～20000mg/L be mixed.In temperature is 24～26 ℃, and light intensity 2000～3000Lx cultivates under 12～14 hours the condition of illumination every day, can take root in 20～30 days.
The present invention compared with prior art, have the following advantages and effect: method is easy, process is short, method of operating can be produced the rosewood seedling in a large number in short-term.
Embodiment
The concrete steps of rosewood tissue culture propagation are as follows:
A, will spend the palmitic acid seed with 0.1% mercuric chloride sterilization 10 minutes, and with aseptic water washing 4～5 times, the seed that disinfects be inserted in the germination medium for preparing, this medium only contains agar 4000mg/L, sugared 10000mg/L; Illumination every day 12 hours, light intensity 2400Lx or 2800Lx, temperature is to cultivate under 24 ℃ the condition; Cultivate that seed can germinate after 2～7 days.
B, grow to the high young sprout of 3cm after will germinateing and downcut, make young sprout constantly breed in the proliferated culture medium that access prepares and form the bud of growing thickly, the medium of this propagation comprises the MS minimal medium of first improvement and the additional composition of proliferated culture medium, the first improvement MS minimal medium composition be (unit: mg/L):
The composition of the first improvement MS minimal medium (unit: mg/L):
Nitric acid ammonia (NH
4NO
3) 800～1650
Potassium nitrate (KNO
3) 850～1900
Calcium chloride dihydrate (CaCl
22H
2O) 400～550
Epsom salt (MgSO
47H
2O) 180～370
Potassium dihydrogen phosphate (KH
2PO
4) 80～170
Potassium iodide (KI) 0.83
Boric acid (H
3BO
3) 6.2
Four water manganese sulphate (MnSO
44H
2O) 22.3
White vitriol (ZnSO47H2O) 8.6
Sodium Molybdate Dihydrate (Na2MoO42H2O) 0.25
Cupric sulfate pentahydrate (CuSO45H2O) 0.025
CoCL2 (CoCl26H2O) 0.025
Ferrous sulfate heptahydrate (FeSO47H2O) 27.8
Disodium ethylene diamine tetraacetate (Na
2EDTA2H
2O) 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
The additional composition of proliferated culture medium is 6-benzyl aminopurine (6-Benzyl aminopurine is called for short BA) 1mg/L, methyl (I-NaphthyIacetic acid is called for short NAA) 0.5mg/L, agar 4000mg/L, sugared 30000mg/L; Illumination cultivation, temperature are 25 ℃, light intensity 2000Lx or 2600Lx, and illumination every day 13 hours, switching in per 30～50 days is once.
C, culture of rootage: when breeding to requirement, the young sprout cutting-out that high 4cm is long inserts the root media for preparing makes the long root of young sprout, this root media comprises the additional composition of the second improvement MS minimal medium and root media, the composition of the second improvement MS minimal medium (unit: mg/L):
NH
4NO
3 550～850
KNO
3 600～1000
CaCl
2·2H
2O 200～350
MgSO
4·7H
2O 100～200
KH
2PO
4 50～90
KI 0.2～0.5
H
3BO
3 2.00～3.0
MnSO
4·4H
2O 7.0～12.0
ZnSO
4·7H
2O 2.5～4.5
Na
2MoO
4·2H
2O 0.08～0.125
CuSO
4·5H
2O 0.008～0.0125
CoCl
2·6H
2O 0.008～0.0125
FeSO
4·7H
2O 9.0～14.0
Na
2·EDTA·2H
2O 12.00～18.00
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
The additional composition of root media is that IBA1 mg/L, NAA0.5 mg/L agar 4000mg/L, sugared 20000mg/L are mixed.In temperature is 26 ℃, and light intensity 2500 Lx or 3000Lx cultivate under 14 hours the condition of illumination every day and can take root in 20～30 days.
Claims (1)
1, the method for the quick breeding of a kind of rosewood the steps include:
A, germination: the rosewood seed with 0.1% mercuric chloride sterilization 8～15 minutes, is used aseptic water washing 4～5 times, the seed of sterilizing is inserted in the germination medium for preparing, medium contains agar 3000～5000mg/L, sugar 10000～20000mg/L;
B, propagation: the tip that will grow to 2～5cm after will germinateing downcuts, and makes the tip constantly breed in the proliferated culture medium that access prepares and forms the bud of growing thickly, and proliferated culture medium comprises the additional composition of the first improvement MS minimal medium and proliferated culture medium;
The composition of the described first improvement MS minimal medium, unit is mg/L:
Nitric acid ammonia 800～1650
Potassium nitrate 850～1900
Calcium chloride dihydrate 400～550
Epsom salt 180～370
Potassium dihydrogen phosphate 80～170
Potassium iodide 0.83
Boric acid 6.2
Four water manganese sulphates 22.3
White vitriol 8.6
Sodium Molybdate Dihydrate 0.25
Cupric sulfate pentahydrate 0.025
CoCL2 0.025
Ferrous sulfate heptahydrate 27.8
Disodium ethylene diamine tetraacetate 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2;
The supplementary element of described proliferated culture medium is: 6-benzyl aminopurine 0.1～3mg/L, methyl 0.01～1.0mg/L, agar 3000～5000mg/L, sugar 20000～30000mg/L;
C, culture of rootage: the tip cutting-out that 3～5cm is long inserts the root media for preparing makes the long root of the tip, and root media comprises the additional composition of the second improvement MS minimal medium and root media;
The described second improvement MS minimal medium composition, unit is mg/L:
NH
4NO
3 550～850
KNO
3 600～1000
CaCl
2·2H
2O 200～350
MgSO
4·7H
2O 100～200
KH
2PO
4 50～90
KI 0.2～0.5
H
3BO
3 2.00～3.0
MnSO
4·4H
2O 7.0～12.0
ZnSO
4·7H
2O 2.5～4.5
Na
2MoO
4·2H
2O 0.08～0.125
CuSO
4·5H
2O 0.008～0.0125
CoCl
2·6H
2O 0.008～0.0125
FeSO
4·7H
2O 9.0～14.0
Na
2·EDTA·2H
2O 12.00～18.00
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2;
The additional composition of described root media is: indolebutyric acid 0.1～2mg/L, methyl 0.01～1mg/L, agar 3000～5000mg/L, sugar 10000～20000mg/L;
D, condition of culture: intensity of illumination 2000～3000Lx, light application time 12～14 hours, 24～26 ℃ of temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100189705A CN100360008C (en) | 2005-06-23 | 2005-06-23 | Quick method for breeding rosewood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100189705A CN100360008C (en) | 2005-06-23 | 2005-06-23 | Quick method for breeding rosewood |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1695429A CN1695429A (en) | 2005-11-16 |
| CN100360008C true CN100360008C (en) | 2008-01-09 |
Family
ID=35348449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100189705A Expired - Fee Related CN100360008C (en) | 2005-06-23 | 2005-06-23 | Quick method for breeding rosewood |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548612B (en) * | 2008-04-03 | 2011-04-27 | 西北农林科技大学 | Method for cultivating nursery stock with ultralong root system |
| CN103283599B (en) * | 2013-05-27 | 2014-12-24 | 何碧珠 | Method for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province |
| CN103444529B (en) * | 2013-08-17 | 2015-04-01 | 福建农林大学 | Method for reproduction and mass propagation of lower axis fragment plants of ormosia hosiei.et wils |
| CN105165627B (en) * | 2015-10-22 | 2018-08-17 | 贵州大学 | A kind of rosewood tissue culture sterilization formula and tissue culture method |
| CN108739376A (en) * | 2018-04-28 | 2018-11-06 | 中国科学院武汉植物园 | Turn round the blue rapid propagation method of valve U.S. hat |
-
2005
- 2005-06-23 CN CNB2005100189705A patent/CN100360008C/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 花榈木育苗技术. 江昌志.林业实用技术，第9期. 2004 * |
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| CN1695429A (en) | 2005-11-16 |
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