CN116349690A - Pesticide composition for preventing and treating passion fruit virus diseases - Google Patents
Pesticide composition for preventing and treating passion fruit virus diseases Download PDFInfo
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- CN116349690A CN116349690A CN202310252493.7A CN202310252493A CN116349690A CN 116349690 A CN116349690 A CN 116349690A CN 202310252493 A CN202310252493 A CN 202310252493A CN 116349690 A CN116349690 A CN 116349690A
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- sitosterol
- passion fruit
- pesticide composition
- virus diseases
- pyraclostrobin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
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Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating passion fruit virus diseases. A pesticide composition for preventing and treating passion fruit virus diseases is prepared by compounding sitosterol, chitosan, glucopolyolefin or pyraclostrobin as effective components. When the effective components in the pesticide composition are compounded according to a certain mass ratio, the pesticide composition has a synergistic effect on the pathogen of passion fruit virus diseases, can improve the control effect of passion fruit virus diseases, can reduce the use amount of the effective components, and reduces the control cost.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating passion fruit virus diseases.
Background
Passion fruit virus disease is the most common disease in passion fruit cultivation, and is also called mosaic disease. The virus disease causes the yield and quality of passion fruit to be reduced, and the virus disease forms a serious threat to the development of passion fruit industry, and becomes one of main factors limiting the development of industry.
At present, the virus types causing passion fruit virus diseases are identified to be more than 20, wherein the virus types are most widely distributed by cucumber mosaic virus CMV, passion fruit lignification virus PWV, east Asia passion fruit virus EAPV, evening primrose mosaic virus TeMV and the like. Cucumber mosaic virus CMV is the most common and harmful major pathogen in viral diseases.
Sitosterol, also known as phytosterol, beta-sitosterol, CAS:83-46-5, the structural formula is as follows:
researches show that sitosterol can be used for preventing and treating crop virus diseases. Although the sitosterol has a certain control effect on crop virus diseases when singly used, the single-component medicament is easy to cause the generation of virus resistance, namely the sitosterol is singly used and has the risk of drug resistance.
Application number CN201310488412.X discloses an antiviral composition containing daucosterol and sitosterol and an antiviral application thereof, and specifically discloses the antiviral composition containing the active ingredients of daucosterol and sitosterol, wherein the weight ratio of the two is 1:50 to 50:1. compared with independent use of the carrot glycoside or the cereal sterol, the antiviral composition disclosed by the invention has obvious synergism on various plant viruses; meanwhile, the service life of the active compound can be prolonged, and the generation of plant virus drug resistance can be delayed.
The inventor finds that when the sitosterol is compounded with chitosan, glucopolyolefin or pyraclostrobin through indoor experiments, the sitosterol has a synergistic effect on the cucumber mosaic virus CMV causing passion fruit virus diseases, not only can the prevention and treatment effect on the virus diseases be improved, but also the drug resistance risk of the sitosterol which is singly used can be reduced.
At present, no report related to the compounding of sitosterol with chitosan, glucopolyolefin or pyraclostrobin is found.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a pesticide composition for preventing and treating passion fruit virus diseases, which aims to solve the problem of drug resistance existing in the independent use of sitosterol.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the pesticide composition is compounded with sitosterol, chitosan, gluco-polyolefin or pyraclostrobin as effective components.
Preferably, the mass ratio of the sitosterol to the chitosan is 1-9:7-1.
Preferably, the mass ratio of the sitosterol to the glucagons is 1-20:15-1.
Preferably, the mass ratio of the sitosterol to the pyraclostrobin is 1-40:10-1.
In addition, the invention also provides application of the pesticide composition in preventing and treating passion fruit virus diseases.
Preferably, the pathogen of the passion fruit virus disease is cucumber mosaic virus CMV.
Compared with the prior art, the invention has the following beneficial effects:
(1) When the effective components in the pesticide composition are compounded according to a certain mass ratio, the pesticide composition has a synergistic effect on the pathogen of passion fruit virus diseases, and compared with single effective components, the pesticide composition can improve the control effect of passion fruit virus diseases, reduce the use amount of the effective components and reduce the control cost.
(2) The pesticide composition can delay the generation of plant virus drug resistance and reduce the drug resistance risk of using single effective component.
Detailed Description
The following description of the embodiments of the present invention will be apparent from the description of the embodiments of the present invention, which is provided in part, but not in whole. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: indoor biological Activity assay
1. Test toxin source: collecting fresh leaves infected with cucumber mosaic virus CMV in a planting field of Passion fruit No. one purple sweet, adding PBS buffer solution (0.01 mol/L, pH 7.2), adding 500 mesh quartz sand, grinding, homogenizing, and filtering residues with 4 layers of gauze to obtain virus liquid for later use. Wherein the ratio of fresh leaf mass to PBS buffer volume is 50mg:1L.
2. Test agent
95% of sitosterol technical, 99% of chitosan technical, 95% of gluco-polyolefin technical and 98% of pyraclostrobin technical are all sold in the market.
The raw materials are dissolved and diluted by 0.1 percent Tween-80 water solution to prepare single-dose mother solution. And setting a plurality of groups of proportions, setting 7 gradient mass concentrations of each single agent and each group of proportion mixture according to an equal ratio method, and preparing all the medicaments at present.
3. Test method
Selecting healthy passion fruit seedlings with uniform growth vigor, pruning old leaves, and reserving 5 leaves for inoculation. Spraying a layer of silicon carbide on the surface of each leaf, and slightly rubbing the whole leaf by dipping virus liquid with a brush for friction inoculation. After inoculation, lightly flushing the surface of the leaf with clear water, spraying the liquid medicine after airing, and spraying once every 7d for 3 times. The culture is carried out in an illumination incubator at the temperature of 25+/-1 ℃. Each of the 30 seedlings was treated with the liquid medicine, repeated 3 times, and a water treatment was sprayed as a blank. The disease condition was investigated before the first and after 7d of the last spraying, and the disease index and the relative control effect were calculated.
4. Disease level meter
Table 1 progression of illness
| Progression of illness state | Disease condition |
| 0 | Disease-free whole plant |
| 1 | The leaves show few fading spots or heart leaves clear veins |
| 3 | Flower and leaf of middle and upper leaf |
| 5 | Middle and upper leaves are heavy, very few leaves shrink or malformation |
| 7 | Most leaves are heavy, part of leaves shrink, malformation or have necrotic spots, and plants are slightly dwarfed |
| 9 | Most leaves are heavy, malformed or necrotic spots, and plants obviously dwarf or even die |
5. Data analysis: regression analysis is carried out on the concentration log value of each treatment medicament and the relative prevention probability value of each treatment by DPS software, and the EC of each treatment medicament is calculated 50 And the co-toxicity coefficient (CTC value) of the mixture was calculated according to the grand cloud Pei method.
6. Evaluation of drug efficacy
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results are shown in tables 2-4.
TABLE 2 results of indoor Activity test of sitosterol and Chitosan Complex against cucumber mosaic Virus CMV
As can be seen from Table 2, sitosterol and chitosan are present in the range of 1-9: within the mass ratio of 7-1, the composition shows a synergistic effect on cucumber mosaic virus CMV.
TABLE 3 results of indoor Activity test of sitosterol and Gluconose complexing against cucumber mosaic Virus CMV
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Sitosterol | 4.0364 | 100.0000 | -- | -- |
| Gluconoids | 6.5233 | 61.8767 | -- | -- |
| Sitosterol 1: gluconose 15 | 7.0555 | 57.2093 | 64.2594 | 89.0287 |
| Sitosterol 1: gluconose 10 | 6.8011 | 59.3492 | 65.3424 | 90.8280 |
| Sitosterol 1: gluconose 5 | 4.6129 | 87.5024 | 68.2305 | 128.2453 |
| Sitosterol 1: gluconose 3 | 3.9910 | 101.1376 | 71.4075 | 141.6344 |
| Sitosterol 1: gluconose 1 | 3.2271 | 125.0782 | 80.9383 | 154.5352 |
| Sitosterol 3: gluconose 1 | 2.2814 | 176.9264 | 90.4692 | 195.5655 |
| Sitosterol 5: gluconose 1 | 1.9422 | 207.8262 | 93.6461 | 221.9272 |
| Sitosterol 10: gluconose 1 | 3.4390 | 117.3713 | 96.5342 | 121.5852 |
| Sitosterol 15: gluconose 1 | 4.0595 | 99.4310 | 97.6173 | 101.8579 |
| Sitosterol 20: gluconose 1 | 4.7721 | 84.5833 | 98.1846 | 86.1472 |
As can be seen from table 3, when sitosterol was compounded with glucopolyolefin, at 1:15-10 and 15-20:1, and shows additive effect on cucumber mosaic virus CMV within the mass ratio range; 1-10: within the mass ratio range of 5-1, the composition shows a synergistic effect on cucumber mosaic virus CMV.
TABLE 4 results of indoor Activity test of sitosterol and pyraclostrobin against cucumber mosaic Virus CMV
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Sitosterol | 4.0364 | 100.0000 | -- | -- |
| Pyraclostrobin | 2.5917 | 155.7433 | -- | -- |
| Sitosterol 1: pyraclostrobin 10 | 2.4183 | 166.9106 | 150.6758 | 110.7747 |
| Sitosterol 1: pyraclostrobin 5 | 1.7963 | 224.7063 | 146.4528 | 153.4326 |
| Sitosterol 1: pyraclostrobin 3 | 0.9508 | 424.5267 | 141.8075 | 299.3683 |
| Sitosterol 1: pyraclostrobin 1 | 2.1204 | 190.3603 | 127.8717 | 148.8682 |
| Sitosterol 3: pyrazolyl ethersMycoester 1 | 2.0801 | 194.0484 | 113.9358 | 170.3137 |
| Sitosterol 5: pyraclostrobin 1 | 3.0001 | 134.5422 | 109.2906 | 123.1050 |
| Sitosterol 10: pyraclostrobin 1 | 2.5999 | 155.2521 | 105.0676 | 147.7641 |
| Sitosterol 15: pyraclostrobin 1 | 3.1154 | 129.5628 | 103.4840 | 125.2009 |
| Sitosterol 20: pyraclostrobin 1 | 1.3914 | 290.0963 | 102.6544 | 282.5950 |
| Sitosterol 30: pyraclostrobin 1 | 0.9662 | 417.7603 | 101.7982 | 410.3809 |
| Sitosterol 40: pyraclostrobin 1 | 0.8852 | 455.9873 | 101.3596 | 449.8709 |
As can be seen from Table 4, the ratio of sitosterol to pyraclostrobin is 1-40: within the mass ratio of 5-1, the composition shows a synergistic effect on cucumber mosaic virus CMV.
In conclusion, when the sitosterol is compounded with chitosan, glucopolyolefin or pyraclostrobin, the sitosterol has a synergistic effect on the cucumber mosaic virus CMV of passion fruit virus diseases, and compared with a single active ingredient, the sitosterol can improve the control effect of passion fruit virus diseases, reduce the use amount of the active ingredient and reduce the control cost.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (6)
1. The pesticide composition is characterized in that the effective component is compounded by sitosterol, chitosan, gluco-polyolefin or pyraclostrobin.
2. A pesticide composition as set forth in claim 1, characterized in that the mass ratio of sitosterol to chitosan is 1-9:7-1.
3. The pesticide composition as set forth in claim 1, wherein the mass ratio of sitosterol to glucide is 1 to 20:15-1.
4. The pesticide composition as set forth in claim 1, wherein the mass ratio of sitosterol to pyraclostrobin is 1-40:10-1.
5. Use of a pesticide composition as set forth in any one of claims 1 to 4 for controlling passion fruit virus diseases.
6. Use according to claim 5, characterized in that the pathogen of passion fruit virus disease is cucumber mosaic virus CMV.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310252493.7A CN116349690A (en) | 2023-03-16 | 2023-03-16 | Pesticide composition for preventing and treating passion fruit virus diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310252493.7A CN116349690A (en) | 2023-03-16 | 2023-03-16 | Pesticide composition for preventing and treating passion fruit virus diseases |
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| Publication Number | Publication Date |
|---|---|
| CN116349690A true CN116349690A (en) | 2023-06-30 |
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|---|---|---|---|
| CN202310252493.7A Pending CN116349690A (en) | 2023-03-16 | 2023-03-16 | Pesticide composition for preventing and treating passion fruit virus diseases |
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| CN (1) | CN116349690A (en) |
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- 2023-03-16 CN CN202310252493.7A patent/CN116349690A/en active Pending
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