CN116172012A - Sterilization composition for preventing and treating powdery mildew of bitter gourd - Google Patents
Sterilization composition for preventing and treating powdery mildew of bitter gourd Download PDFInfo
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- CN116172012A CN116172012A CN202310169917.3A CN202310169917A CN116172012A CN 116172012 A CN116172012 A CN 116172012A CN 202310169917 A CN202310169917 A CN 202310169917A CN 116172012 A CN116172012 A CN 116172012A
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- Prior art keywords
- powdery mildew
- preventing
- tetrazolium
- picolinate
- melatonin
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- 241000221785 Erysiphales Species 0.000 title claims abstract description 42
- 235000009811 Momordica charantia Nutrition 0.000 title claims abstract description 32
- 239000000203 mixture Substances 0.000 title claims abstract description 21
- 235000008322 Trichosanthes cucumerina Nutrition 0.000 title claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 title description 2
- 238000004659 sterilization and disinfection Methods 0.000 title description 2
- 244000078912 Trichosanthes cucumerina Species 0.000 title 1
- 244000302512 Momordica charantia Species 0.000 claims abstract description 31
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 claims abstract description 23
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229960003987 melatonin Drugs 0.000 claims abstract description 23
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 22
- 235000009812 Momordica cochinchinensis Nutrition 0.000 claims abstract description 17
- 235000018365 Momordica dioica Nutrition 0.000 claims abstract description 17
- 239000005788 Fluxapyroxad Substances 0.000 claims abstract description 11
- SXSGXWCSHSVPGB-UHFFFAOYSA-N fluxapyroxad Chemical compound FC(F)C1=NN(C)C=C1C(=O)NC1=CC=CC=C1C1=CC(F)=C(F)C(F)=C1 SXSGXWCSHSVPGB-UHFFFAOYSA-N 0.000 claims abstract description 11
- 150000003536 tetrazoles Chemical class 0.000 claims abstract description 9
- BINQFWQYTCAARG-UHFFFAOYSA-N 1-(1-acetylindolizin-3-yl)ethanone Chemical compound C1=CC=CC2=C(C(=O)C)C=C(C(C)=O)N21 BINQFWQYTCAARG-UHFFFAOYSA-N 0.000 claims abstract description 6
- SIOXPEMLGUPBBT-UHFFFAOYSA-M picolinate Chemical compound [O-]C(=O)C1=CC=CC=N1 SIOXPEMLGUPBBT-UHFFFAOYSA-M 0.000 claims description 38
- 125000003831 tetrazolyl group Chemical group 0.000 claims description 36
- WKBPZYKAUNRMKP-UHFFFAOYSA-N 1-[2-(2,4-dichlorophenyl)pentyl]1,2,4-triazole Chemical compound C=1C=C(Cl)C=C(Cl)C=1C(CCC)CN1C=NC=N1 WKBPZYKAUNRMKP-UHFFFAOYSA-N 0.000 claims description 3
- 239000005813 Penconazole Substances 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 7
- 239000000575 pesticide Substances 0.000 abstract description 5
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- GOFJDXZZHFNFLV-UHFFFAOYSA-N 5-fluoro-1,3-dimethyl-N-[2-(4-methylpentan-2-yl)phenyl]pyrazole-4-carboxamide Chemical compound CC(C)CC(C)C1=CC=CC=C1NC(=O)C1=C(F)N(C)N=C1C GOFJDXZZHFNFLV-UHFFFAOYSA-N 0.000 abstract description 3
- 239000005815 Penflufen Substances 0.000 abstract description 3
- 239000005869 Pyraclostrobin Substances 0.000 abstract description 3
- 238000013329 compounding Methods 0.000 abstract description 3
- YHSAMQAMZAWZPE-UHFFFAOYSA-N butyl 2-[4-[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxyphenoxy]propanoate Chemical group C1=CC(OC(C)C(=O)OCCCC)=CC=C1OC1=NC=C(C(F)(F)F)C=C1Cl YHSAMQAMZAWZPE-UHFFFAOYSA-N 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 10
- 241000193403 Clostridium Species 0.000 description 6
- 244000052616 bacterial pathogen Species 0.000 description 6
- KYKAJFCTULSVSH-UHFFFAOYSA-N chloro(fluoro)methane Chemical compound F[C]Cl KYKAJFCTULSVSH-UHFFFAOYSA-N 0.000 description 6
- 239000003899 bactericide agent Substances 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 239000013043 chemical agent Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- BSKHPKMHTQYZBB-UHFFFAOYSA-N 2-methylpyridine Chemical compound CC1=CC=CC=N1 BSKHPKMHTQYZBB-UHFFFAOYSA-N 0.000 description 3
- 241000317981 Podosphaera fuliginea Species 0.000 description 3
- 229930182558 Sterol Natural products 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
- 229920000053 polysorbate 80 Polymers 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 150000003432 sterols Chemical class 0.000 description 3
- 235000003702 sterols Nutrition 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 244000241257 Cucumis melo Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- URHWNXDZOULUHC-ULJHMMPZSA-N picarbutrazox Chemical compound CN1N=NN=C1\C(C=1C=CC=CC=1)=N/OCC1=CC=CC(NC(=O)OC(C)(C)C)=N1 URHWNXDZOULUHC-ULJHMMPZSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- -1 tetrazolium picolinate compound Chemical class 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- PPDBOQMNKNNODG-NTEUORMPSA-N (5E)-5-(4-chlorobenzylidene)-2,2-dimethyl-1-(1,2,4-triazol-1-ylmethyl)cyclopentanol Chemical compound C1=NC=NN1CC1(O)C(C)(C)CC\C1=C/C1=CC=C(Cl)C=C1 PPDBOQMNKNNODG-NTEUORMPSA-N 0.000 description 1
- ZRYJCBRMUIHLOV-UHFFFAOYSA-N 2-methylpyridine 2H-tetrazole Chemical compound N1N=NN=C1.CC1=NC=CC=C1 ZRYJCBRMUIHLOV-UHFFFAOYSA-N 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 241000896246 Golovinomyces cichoracearum Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 241000143639 Monocystis Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000233654 Oomycetes Species 0.000 description 1
- 239000005592 Penoxsulam Substances 0.000 description 1
- SYJGKVOENHZYMQ-UHFFFAOYSA-N Penoxsulam Chemical compound N1=C2C(OC)=CN=C(OC)N2N=C1NS(=O)(=O)C1=C(OCC(F)F)C=CC=C1C(F)(F)F SYJGKVOENHZYMQ-UHFFFAOYSA-N 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 239000005842 Thiophanate-methyl Substances 0.000 description 1
- 239000005859 Triticonazole Substances 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- MFSWTRQUCLNFOM-UHFFFAOYSA-N methyl 2-(4-{[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy}phenoxy)propanoate Chemical group C1=CC(OC(C)C(=O)OC)=CC=C1OC1=NC=C(C(F)(F)F)C=C1Cl MFSWTRQUCLNFOM-UHFFFAOYSA-N 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the technical field of pesticides, and in particular relates to a bactericidal composition for preventing and treating powdery mildew of balsam pear. The bactericidal composition for preventing and treating powdery mildew of balsam pear is prepared by compounding tetrazole picolide, fluxapyroxad, penflufen or melatonin. The bactericidal composition has a synergistic effect on preventing and treating powdery mildew of bitter gourd after the effective components of tetrazole-pyraclostrobin and fluxapyroxad, haloxyfop-butyl or melatonin are compounded, can improve the preventing and treating effect of powdery mildew of bitter gourd, and can be used for developing novel efficient medicaments for preventing and treating powdery mildew of bitter gourd.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating powdery mildew of balsam pear.
Background
Powdery mildew of balsam pear can occur from seedling stage to harvest stage, and mainly damages plant leaves. The balsam pear powdery mildew is generated in the front and back of the leaf at the beginning of the onset, the powder spots are gradually enlarged into continuous white powder spots with insignificant edges, when serious, the powder is distributed over the whole leaf, and the leaf is gradually withered and yellow to lose photosynthesis function, thus causing serious quality and yield loss. Pathogenic bacteria causing balsam pear powdery mildew in China mainly comprise powdery mildew (Erysiphe cichoracearum) of powdery mildew genus and powdery mildew (Sphaerotheca fuliginea) of monocystis genus, and S.fuliginea is common.
At present, the main way of controlling powdery mildew is to spray chemical agents, but powdery mildew pathogenic bacteria have different resistances to the existing various chemical agents along with long-term use of the agents, so that the control effect of the existing various agents on powdery mildew is reduced. For example, powdery mildew pathogenic bacteria have developed higher resistance to endo-benzimidazole bactericides such as carbendazim and thiophanate-methyl. Therefore, it is necessary to screen efficient balsam pear powdery mildew prevention and treatment agents.
Tetrazolium picolinate english generic name: picarbutrazox; CAS number: 500207-04-5; the structural formula is as follows:
tetrazolium picornase is a carbamate bactericide developed by the Nippon Cao Co., ltd., and has unique structure, novel action matrix and permeation and treatment effects. The tetrazolium picolinate has the action mechanism of reducing sporangia formation and zoospore number by inhibiting the formation of germ cell membrane, inhibiting hypha growth and spore germination, and preventing and treating oomycete diseases. Application number CN201511015622.2 discloses a composition of picrbutanzox and sterol biosynthesis inhibitor bactericides, a preparation and application thereof, and specifically discloses a composition of picrbutanzox and sterol biosynthesis inhibitor bactericides, namely, triticonazole or fluoxynil, wherein the mass ratio of the picrbutanzox to the sterol biosynthesis inhibitor bactericides, namely, the triflurazitol is 1:15-80: the compound in the formula 1 has synergism and can be used for preventing and treating powdery mildew of tobacco, sesame, sunflower and melons.
At present, no report on the compounding of tetrazolium picolinate with fluxapyroxad, penflufen-sodium or melatonin is seen.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and treating powdery mildew of balsam pear, which aims to solve the problem that powdery mildew pathogenic bacteria have different resistances to the existing various chemical agents along with long-term use of the agents, so that the preventing and treating effect of the existing various agents on powdery mildew is reduced.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the bactericidal composition for preventing and treating powdery mildew of balsam pear is prepared by compounding tetrazole picolide, fluxapyroxad, penflufen or melatonin.
Preferably, the mass ratio of the tetrazole picolide to the fluxapyroxad is 1-4:16-1.
Preferably, the mass ratio of the tetrazole picoline to the penconazole is 1-9:7-1.
Preferably, the mass ratio of the tetrazolium picolinate to the melatonin is 1-27:19-1.
The invention also provides application of the bactericidal composition in prevention and control of powdery mildew of balsam pear.
Compared with the prior art, the invention has the following beneficial effects:
(1) The bactericidal composition has a synergistic effect on preventing and treating powdery mildew of bitter gourd after the effective components of tetrazole-pyraclostrobin and fluxapyroxad, haloxyfop-butyl or melatonin are compounded, can improve the preventing and treating effect of powdery mildew of bitter gourd, and can be used for developing novel efficient medicaments for preventing and treating powdery mildew of bitter gourd.
(2) The bactericidal composition has a different bactericidal mechanism between the tetrazole picoline and other effective components, can delay the development of drug resistance of pathogenic bacteria, prolongs the service life of chemical agents, and can effectively reduce the risk of drug resistance of single effective component.
Detailed Description
The invention will be better understood from the following examples. However, it will be readily appreciated by those skilled in the art that the description of the embodiments is provided for illustration only and should not limit the invention as described in detail in the claims.
Examples: indoor biological activity determination of tetrazolium picolinate compound
1. Test crop
The bitter gourd variety of the green bamboo is cultivated in a pot until the period of 4 leaves, and each pot is provided with 3 plants, and numbering is reserved.
2. Test agent
97% of tetrazolium ammonia ester raw medicine, 98% of fluxapyroxad raw medicine, 97% of penflufen bacteria, 99% of melatonin (CAS number: 73-31-4) are all sold in the market.
3. Test method (refer to NY/T1156.11-2008, section 11 of pesticide, test method for controlling powdery mildew of melon, section 11 of pesticide, biological assay test guidelines in pesticide laboratory).
3.1. Preparation of spore suspension: the balsam pear leaves infected with powdery mildew in the balsam pear planting field are collected and are separated, purified and identified in a laboratory to be determined as the single-capsule shell powdery mildew (Sphaerotheca fuliginea). Inoculating the separated and purified pathogenic bacteria onto the tested crop, washing fresh spores on leaf with purified water containing 0.1% Tween-80 after the tested crop is ill, and filtering with double-layer gauze to obtain 1×10 5 The suspension of the spores/mL is reserved;
3.2 preparing a medicament: dissolving the test agent with dimethyl sulfoxide, diluting with 0.1% Tween-80 aqueous solution to obtain single-agent mother solution, and setting multiple groups of ratios, wherein each single-agent and the ratio mixture have 5 mass concentration gradients according to an equal ratio method.
3.3 inoculation and drug treatment: spraying and inoculating the tested crops with the spore suspension 24h before the medicament treatment; after natural air drying, the liquid medicine is uniformly sprayed on the leaf surfaces of the crops to be tested until the leaves are totally wet, each treatment is repeated for 3 times, and the treatment only containing 0.1% of Tween-80 aqueous solution is used as a blank control; after natural air drying, transferring to a thermostatic chamber, and culturing for 7d at the temperature of 24 ℃;
3.4 investigation and calculation:
and when the leaf rate of the blank control reaches more than 80%, the disease conditions of each treatment are investigated in a grading way, 40 leaves are investigated per treatment, and the disease index and the prevention and treatment effect of each treatment are calculated. Wherein, the grading standard is shown in Table 1.
TABLE 1 disease grade assessment criteria
4. Data analysis: performing data statistical analysis by adopting DPS software, and performing linear regression with the disinfectant concentration logarithmic value x and the corresponding control efficiency numerical value y to obtain a virulence regression equation and virulence EC of the medicament on target bacteria 50 Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.
5. Evaluation of drug efficacy
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results are shown in tables 2-4.
TABLE 2 determination of indoor biological Activity of tetrazolium picolinate and Fluoxazole amide complex against Momordica Charantia powdery mildew
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Tetrazolium picolinate | 43.3741 | 100.0000 | -- | -- |
| Fluoxapyroxad | 18.5906 | 233.3120 | -- | -- |
| Tetrazolium picolinate 1: fluoxapyroxad 16 | 11.3017 | 383.7839 | 225.4701 | 170.2150 |
| Tetrazolium picolinate 1: fluoxapyroxad 12 | 9.0832 | 477.5200 | 223.0572 | 214.0796 |
| Tetrazolium picolinate 1: fluoxapyroxad 8 | 6.1494 | 705.3387 | 218.4996 | 322.8101 |
| Tetrazolium picolinate 1: fluoxapyroxad 4 | 13.4102 | 323.4411 | 206.6496 | 156.5167 |
| Tetrazolium picolinate 1: fluoxapyroxad 2 | 17.1986 | 252.1955 | 188.8747 | 133.5253 |
| Tetrazolium picolinate 1: fluoxapyroxad 1 | 20.6017 | 210.5365 | 166.6560 | 126.3300 |
| Tetrazolium picolinate 2: fluoxapyroxad 1 | 16.0626 | 270.0316 | 144.4373 | 186.9542 |
| Tetrazolium picolinate 4: fluoxapyroxad 1 | 24.5564 | 176.6305 | 126.6624 | 139.4499 |
As shown in table 2, the effective components of tetrazole picolide and fluxapyroxad are compounded in a mass ratio of 1-4: the co-toxicity coefficient of the balsam pear powdery mildew in the 16-1 is more than 120, and the synergism is shown.
TABLE 3 determination of indoor biological Activity of tetrazolium picolinate and penoxsulam in combination with Momordica Charantia powdery mildew
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Tetrazolium picolinate | 43.3741 | 100.0000 | -- | -- |
| Clostridium chlorofluorocarbon | 2.1647 | 2003.7003 | -- | -- |
| Tetrazolium picolinate 1: clocymoxanil 7 | 1.6527 | 2624.4388 | 1765.7377 | 148.6313 |
| Tetrazolium picolinate 1: clocymoxanil 5 | 2.0478 | 2118.0828 | 1686.4169 | 125.5966 |
| Tetrazolium picolinate 1: clocymoxanil 3 | 2.1344 | 2032.1449 | 1527.7752 | 133.0133 |
| Tetrazolium picolinate 1: clostridium chlorofluorocarbon 1 | 2.9257 | 1482.5204 | 1051.8501 | 140.9441 |
| Tetrazolium picolinate 3: clostridium chlorofluorocarbon 1 | 3.8684 | 1121.2413 | 575.9251 | 194.6853 |
| Tetrazolium picolinate 5: clostridium chlorofluorocarbon 1 | 5.6677 | 765.2857 | 417.2834 | 183.3971 |
| Tetrazolium picolinate 7: clostridium chlorofluorocarbon 1 | 7.8880 | 549.8745 | 337.9625 | 162.7028 |
| Tetrazolium picolinate 9: clostridium chlorofluorocarbon 1 | 12.1157 | 357.9991 | 290.3700 | 123.2907 |
As shown in Table 3, the effective components of tetrazole picoline and penconazole are compounded in a mass ratio of 1-9: the co-toxicity coefficient of 7-1 to the powdery mildew of balsam pear is more than 120, and the synergistic effect is shown.
Table 4 determination of indoor bioactivity of tetrazolium picolinate and melatonin in combination against powdery mildew of balsam pear
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Tetrazolium picolinate | 43.3741 | 100.0000 | -- | -- |
| Melatonin | 62.7813 | 69.0876 | -- | -- |
| Tetrazolium picolinate 1: melatonin 19 | 48.5491 | 89.3407 | 70.6332 | 126.4853 |
| Tetrazolium picolinate 1: melatonin 9 | 40.1785 | 107.9535 | 72.1788 | 149.5639 |
| Tetrazolium picolinate 1: melatonin 7 | 43.9686 | 98.6479 | 72.9517 | 135.2237 |
| Tetrazolium picolinate 1: melatonin 5 | 28.8649 | 150.2659 | 74.2397 | 202.4065 |
| Tetrazolium picolinate 1: melatonin 1 | 35.2594 | 123.0143 | 84.5438 | 145.5036 |
| Tetrazolium picolinate 5: melatonin 1 | 37.1957 | 116.6105 | 94.8479 | 122.9447 |
| Tetrazolium picolinate 7: melatonin 1 | 24.2292 | 179.0158 | 96.1360 | 186.2111 |
| Tetrazolium picolinate 9: melatonin 1 | 37.1742 | 116.6780 | 96.9088 | 120.3998 |
| Tetrazolium picolinate 18: melatonin 1 | 30.2069 | 143.5900 | 98.3730 | 145.9648 |
| Tetrazolium picolinate 27: melatonin 1 | 19.2496 | 225.3247 | 98.8960 | 227.8401 |
As shown in table 4, the effective components of tetrazole-picoline and melatonin are compounded in a mass ratio of 1-27: the co-toxicity coefficient of the balsam pear powdery mildew in the 19-1 is more than 120, and the synergism is shown.
In conclusion, the bactericidal composition has a synergistic effect on preventing and treating powdery mildew of bitter gourd after the effective components of tetrazole-pyraclostrobin and fluxapyroxad, haloxyfop-methyl or melatonin are compounded, can improve the preventing and treating effect of powdery mildew of bitter gourd, and can be used for developing novel efficient medicaments for preventing and treating powdery mildew of bitter gourd.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (5)
1. The bactericidal composition for preventing and treating powdery mildew of balsam pear is characterized in that the active ingredients of the bactericidal composition are compounded by tetrazole picolide and fluxapyroxad, penflufen-methyl or melatonin.
2. The bactericidal composition of claim 1, wherein the mass ratio of tetrazolium picolinate to fluxapyroxad is 1-4:16-1.
3. The bactericidal composition of claim 1, wherein the mass ratio of tetrazole picolide to penconazole is 1-9:7-1.
4. The bactericidal composition of claim 1, wherein the mass ratio of tetrazolium picolinate to melatonin is from 1 to 27:19-1.
5. Use of a bactericidal composition according to any of claims 1-4 for controlling powdery mildew in bitter gourd.
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