CN116473070B - Biological sterilization composition for preventing and treating powdery mildew of strawberries - Google Patents
Biological sterilization composition for preventing and treating powdery mildew of strawberries Download PDFInfo
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- CN116473070B CN116473070B CN202310305685.XA CN202310305685A CN116473070B CN 116473070 B CN116473070 B CN 116473070B CN 202310305685 A CN202310305685 A CN 202310305685A CN 116473070 B CN116473070 B CN 116473070B
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- powdery mildew
- strawberries
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- 241000221785 Erysiphales Species 0.000 title claims abstract description 30
- 235000021012 strawberries Nutrition 0.000 title claims abstract description 21
- 239000000203 mixture Substances 0.000 title claims abstract description 18
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 15
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 14
- 240000009088 Fragaria x ananassa Species 0.000 title claims 2
- WUOACPNHFRMFPN-UHFFFAOYSA-N alpha-terpineol Chemical compound CC1=CCC(C(C)(C)O)CC1 WUOACPNHFRMFPN-UHFFFAOYSA-N 0.000 claims abstract description 15
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229940093265 berberine Drugs 0.000 claims abstract description 15
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229920001184 polypeptide Polymers 0.000 claims abstract description 14
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 14
- 230000003115 biocidal effect Effects 0.000 claims 1
- 241000220223 Fragaria Species 0.000 abstract description 37
- 235000016623 Fragaria vesca Nutrition 0.000 abstract description 17
- 235000011363 Fragaria x ananassa Nutrition 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 13
- 239000000575 pesticide Substances 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 3
- 238000013329 compounding Methods 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 16
- 239000003814 drug Substances 0.000 description 9
- 229940079593 drug Drugs 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000013043 chemical agent Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 241000235349 Ascomycota Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000143639 Monocystis Species 0.000 description 1
- 241000209046 Pennisetum Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a biological sterilization composition for preventing and treating powdery mildew of strawberries. The invention provides a biological sterilization composition for preventing and treating powdery mildew of strawberries, which is prepared by compounding an active ingredient of component A and beta-lupin conglutin polypeptide. The biological sterilization composition disclosed by the invention has the advantages that the berberine and the terpene alcohol are compounded with the beta-lupin conglutin polypeptide in a certain mass ratio within a certain proportioning range, so that the prevention effect on strawberry powdery mildew can be obviously improved. The berberine, terpene alcohol and beta-lupin conglutin polypeptide belong to plant source biological pesticides, are easy to degrade and not easy to remain, cannot cause biological enrichment phenomenon, and are safe to agricultural products and environment.
Description
Technical Field
The invention belongs to the technical field of pesticides, and in particular relates to a biological sterilization composition for preventing and treating powdery mildew of strawberries
Background
The powdery mildew of the strawberries is an important common disease in strawberry production, the incidence rate is high, the damage is serious, the yield is generally reduced by 20% -30% after the powdery mildew of the strawberries occurs, the yield is reduced by 50% after the serious damage is caused, and even the yield and the quality of the strawberries are seriously influenced. The pathogenic bacteria of strawberry powdery mildew are ascomycota fungi of ascomycota monocystis of pennisetum. Strawberry powdery mildew can occur from the seedling stage to the fruit maturity stage, and continuous cropping is carried out in a greenhouse for many years, so that pathogenic bacteria in the greenhouse and soil are accumulated in successive years, and therefore, the powdery mildew of the strawberry is early and serious in onset; the ventilation and light transmittance of greenhouse facilities are poor, the planting density is too high, the temperature and the humidity are suitable for the greenhouse, and the occurrence of strawberry powdery mildew is more easy to aggravate.
In the production of the strawberry powdery mildew control, chemical agents are mainly used, and although the chemical agents have certain control effect, the chemical agents are used in a large amount, so that the strawberry powdery mildew has drug resistance, the control difficulty is increased, meanwhile, the plant itself is also damaged, residues exist in the strawberry, the health of people is endangered, and the environment is polluted. Therefore, the novel pesticide for preventing and treating the powdery mildew of the strawberries is developed, the prevention effect is high, the drug residues on the strawberries are reduced, the medication safety is improved, the environmental pressure is reduced, and the problem which is needed to be solved by the technicians in the field is solved.
Disclosure of Invention
In order to solve the technical problems, the invention provides a biological sterilization composition for preventing and treating powdery mildew of strawberries, which is a biological pesticide, has high effect of preventing powdery mildew of strawberries, and is safe to strawberries and environment.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the invention provides a biological sterilization composition for preventing and treating powdery mildew of strawberries, which comprises the effective components of component A and beta-lupin conglutin polypeptide, wherein the mass ratio of the component A to the beta-lupin conglutin polypeptide is 1:15-25:1; preferably, the mass ratio is 1:2-2:1.
The component A consists of berberine and terpene alcohol, and the mass ratio of the berberine to the terpene alcohol is 1:30-20:1; preferably, the mass ratio is 1:4-5:1.
The biological sterilization composition for preventing and treating the powdery mildew of the strawberries has a good preventing and treating effect on the powdery mildew of the strawberries.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the biological sterilization composition, when the mass ratio of berberine to terpene alcohol is 1:4-5:1 and the mass ratio of component A to beta-lupin conglutin polypeptide is 1:2-2:1, the synergy coefficient is greater than 2.1, the synergy effect is obvious, the berberine and terpene alcohol are compounded with the beta-lupin conglutin polypeptide in a certain mass ratio within a certain proportion range, and the prevention effect on strawberry powdery mildew can be obviously improved.
(2) The berberine, terpene alcohol and beta-lupin conglutin polypeptide belong to plant source biological pesticides, are easy to degrade and not easy to remain, cannot cause biological enrichment phenomenon, and are safe to agricultural products and environment. The method is used for preventing and controlling powdery mildew of strawberries, improving the quality and safety of strawberries and reducing the environmental pressure.
Detailed Description
The present invention will be further illustrated with reference to the following examples, but the present invention is not limited to the following examples.
Examples: indoor activity determination of biological sterilization composition for preventing and treating powdery mildew of strawberries on powdery mildew of strawberries
Test crop: strawberry.
Test target: powdery mildew of strawberry.
The experimental method comprises the following steps: the spore suspension spray inoculation method is adopted. Dissolving berberine crude drug and beta-lupin conglutin polypeptide crude drug with distilled water respectively, dissolving terpene alcohol crude drug with ethanol, diluting each drug after dissolution with 0.1% Tween-80 aqueous solution to obtain mother solution, and preparing the mother solution into 5 concentration gradient single and compound drugs respectively with 0.1% Tween-80 aqueous solution. Selecting uniform potted strawberry seedlings, spraying the prepared medicinal liquid, and repeating for 4 times each time, and repeating for 4 pots each time. After 24 hours, taking strawberry leaves full of powdery mildew, gently washing fresh spores on the back of the leaves with sterile water, filtering with double-layer gauze to prepare spore suspension, and spraying and inoculating. And (3) transferring the inoculated strawberry plants to a greenhouse at 23 ℃, and calculating the control effect by using the disease index when the disease is fully developed without the medicament for grading disease investigation. Regression analysis is carried out according to the concentration log value of each medicament and the corresponding control effect rate value, and the EC of each medicament is calculated 50 The interaction of the blends was evaluated according to the Wadley method, wherein SR = 0.5-1.5, the two agents were compounded with additive effect; SR is less than or equal to 0.5, and antagonism is achieved; SR is more than or equal to 1.5, and the synergy is achieved. The classification method and the calculation formula are as follows.
The grading method comprises the step of taking the disease spot area of each leaf as a percentage of the whole leaf area.
Level 0: no disease;
stage 1: the area of the disease spots accounts for less than 5% of the area of the whole leaf;
3 stages: the area of the disease spots accounts for 6% -15% of the area of the whole leaf;
5 stages: the area of the disease spots accounts for 16% -25% of the area of the whole leaf;
7 stages: the area of the disease spots accounts for 26% -50% of the area of the whole leaf;
stage 9: the area of the disease spots accounts for more than 51% of the area of the whole leaf.
Disease index=100×Σ (leaf number of each stage disease×relative stage number)/(total leaf number of investigation×9)
Control effect (%) =100× (control disease index-treated disease index)/control disease index.
EC 50 (theoretical value) = (a+b)/(a/EC) 50a +b/EC 50b )
SR＝EC 50 (theoretical value)/EC 50 (actual value)
Wherein SR is the synergistic coefficient
a. b is the percentage of each component in the mixture.
TABLE 1 indoor Activity determination results of biological sterilizing composition for preventing and controlling powdery mildew of strawberry
As can be seen from table 1, the synergy coefficients of the component a and the beta-lupin conglutin polypeptide in the mass ratio of 1:15-25:1 are both greater than 1.5, which indicates that the component a and the beta-lupin conglutin polypeptide have synergy effects on strawberry powdery mildew after being compounded; the synergistic coefficient of the berberine and the terpene alcohol in the mass ratio of 1:30-20:1 is larger than 1.5, which indicates that the berberine and the terpene alcohol have synergistic effect on powdery mildew of strawberry after being compounded; especially when the mass ratio of berberine to terpene alcohol is 1:4-5:1 and the mass ratio of component A to beta-lupin conglutin polypeptide is 1:2-2:1, the synergy coefficient is greater than 2.1, which indicates that the synergy of the compounding of berberine and terpene alcohol with beta-lupin conglutin polypeptide in a certain ratio range is remarkable.
While the invention has been described in detail with respect to the general description and specific embodiments thereof, it will be apparent to those skilled in the art that various modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (3)
1. The biological sterilization composition for preventing and treating the powdery mildew of the strawberries is characterized in that the effective components of the biological sterilization composition consist of a component A and a beta-lupin conglutin polypeptide;
the component A consists of berberine and terpene alcohol;
the mass ratio of the component A to the beta-lupin conglutin is 1:15-25:1;
the mass ratio of the berberine to the terpene alcohol is 1:30-20:1.
2. The biocidal composition according to claim 1, wherein the mass ratio of component a to β -lupin conglutin is 1:2 to 2:1.
3. The biological sterilization composition according to claim 1, wherein the mass ratio of berberine to terpene alcohol is 1:4-5:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310305685.XA CN116473070B (en) | 2023-03-27 | 2023-03-27 | Biological sterilization composition for preventing and treating powdery mildew of strawberries |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310305685.XA CN116473070B (en) | 2023-03-27 | 2023-03-27 | Biological sterilization composition for preventing and treating powdery mildew of strawberries |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116473070A CN116473070A (en) | 2023-07-25 |
| CN116473070B true CN116473070B (en) | 2024-03-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310305685.XA Active CN116473070B (en) | 2023-03-27 | 2023-03-27 | Biological sterilization composition for preventing and treating powdery mildew of strawberries |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108378069A (en) * | 2018-05-09 | 2018-08-10 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of bactericidal composition |
| CN108391664A (en) * | 2018-04-24 | 2018-08-14 | 江苏丘陵地区镇江农业科学研究所 | A kind of plant source bactericidal composition and its application |
| CN108477185A (en) * | 2018-04-19 | 2018-09-04 | 张月婵 | Bactericidal composition containing β-conglutin polypeptide and Er Lv Evil bacterium azoles |
| CN108633921A (en) * | 2018-04-19 | 2018-10-12 | 张友兰 | Bactericidal composition containing β-conglutin polypeptide and oxazole |
| CN112385662A (en) * | 2020-11-18 | 2021-02-23 | 广西壮族自治区农业科学院 | Pesticide composition for preventing and treating powdery mildew of bitter gourds |
-
2023
- 2023-03-27 CN CN202310305685.XA patent/CN116473070B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477185A (en) * | 2018-04-19 | 2018-09-04 | 张月婵 | Bactericidal composition containing β-conglutin polypeptide and Er Lv Evil bacterium azoles |
| CN108633921A (en) * | 2018-04-19 | 2018-10-12 | 张友兰 | Bactericidal composition containing β-conglutin polypeptide and oxazole |
| CN108391664A (en) * | 2018-04-24 | 2018-08-14 | 江苏丘陵地区镇江农业科学研究所 | A kind of plant source bactericidal composition and its application |
| CN108378069A (en) * | 2018-05-09 | 2018-08-10 | 惠州市梦芭迪工业产品设计中心(普通合伙) | A kind of bactericidal composition |
| CN112385662A (en) * | 2020-11-18 | 2021-02-23 | 广西壮族自治区农业科学院 | Pesticide composition for preventing and treating powdery mildew of bitter gourds |
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| CN116473070A (en) | 2023-07-25 |
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