CN116158440A

CN116158440A - Composition for improving thousand seed weight of wheat

Info

Publication number: CN116158440A
Application number: CN202310099109.4A
Authority: CN
Inventors: 张志勇; 熊淑萍; 韦一昊; 王小纯; 马新明
Original assignee: Henan Agricultural University
Current assignee: Henan Agricultural University
Priority date: 2023-02-11
Filing date: 2023-02-11
Publication date: 2023-05-26

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a composition for improving thousand seed weight of wheat. The active ingredients of the sterilization composition are compounded by murrayone and metconazole, tebuconazole or prothioconazole. When the murrayone and other effective components in the bactericidal composition are compounded, the bactericidal composition has a synergistic effect on pathogenic bacteria of wheat powdery mildew within a certain mass ratio range, can improve the control effect on the wheat powdery mildew, reduces the influence of the wheat powdery mildew on thousand seed weight of wheat, and further is beneficial to improving the thousand seed weight of the wheat. Secondly, when the murrayone and other active ingredients in the bactericidal composition are compounded, the development of drug resistance of pathogenic bacteria can be effectively delayed, so that the drug resistance risk brought by the application of a single active ingredient can be reduced. In addition, the bactericidal composition can reduce the application amount of the active ingredients and the control cost, and can provide support for developing novel medicaments.

Description

Composition for improving thousand seed weight of wheat

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to a composition for improving thousand seed weight of wheat.

Background

Wheat powdery mildew is a worldwide disease caused by the specialization of wheat (Bluemeriagramine f.sp.tritici) of Bluegrass, and is a transitive parasitic infection of wheat. Yellow spots are visible in the early stage of disease, and a powdery mildew layer is formed on the surface of the disease gradually develops into a disease spot along with the serious disease. When the illness state is lighter, the mildew spots are distributed in a dispersed way; as the disease progresses, the mildew spots gradually expand into pieces and finally cover Quan She; the lower part of the disease spot and surrounding tissues fade, the disease She Fahuang and early blight, the involvement of stems and leaf sheaths can lead to the lodging of the whole plant, the dwarf, the fine and weak plant, the small spike and small grain, the obvious drop of thousand grain weight and finally the influence on the wheat yield.

In agricultural production, the disease-resistant variety is the most economical means for preventing and controlling wheat powdery mildew, but the disease resistance of the disease-resistant variety is lost after a plurality of years of large-area popularization and planting due to the rapid propagation variation speed of the wheat powdery mildew. At present, the most important measure for preventing and treating wheat powdery mildew is chemical prevention, and the commonly used chemical agents are triazole agents such as triazolone, but the powdery mildew has different degrees of drug resistance to a plurality of existing chemical agents due to long-term unreasonable use of single effective component chemical agents. In order to improve the control effect on powdery mildew, reduce the influence of powdery mildew on thousand seed weight of wheat, and reduce the development speed of pathogen drug resistance, it is necessary to develop a novel medicament.

Murrayone, CAS number: 19668-69-0, molecular formula: c (C) ₁₅ H ₁₄ O ₄ Is derived from Rutaceae plant. The molecular structural formula is as follows:

CN201510815853.5 discloses the use of a natural compound murrayone as an agricultural bactericide, and in the specification, murrayone is disclosed to have control effects on a plurality of diseases such as sclerotinia rot of colza, sheath blight of rice, gray mold of strawberry, scab of wheat, anthracnose of chilli, early blight of tomato and the like.

The inventor finds that the murrayone, metconazole, tebuconazole or prothioconazole have synergism for preventing and treating powdery mildew of wheat in a certain mass ratio range after being compounded by indoor biological activity experiments. At present, no report on the compounding of the murrayone and the metconazole, the tebuconazole or the prothioconazole exists yet.

The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.

Disclosure of Invention

The invention aims to provide a composition for improving thousand seed weight of wheat, which can improve the control effect on wheat powdery mildew compared with single active ingredient, thereby being beneficial to reducing the influence of the powdery mildew on the thousand seed weight of the wheat, and can delay the development of drug resistance of pathogenic bacteria at the same time, and further provide support for developing novel medicaments.

In order to achieve the above purpose, the present invention provides the following technical solutions:

the first object of the invention is to provide a sterilization composition, wherein the effective component of the sterilization composition is compounded by murrayone and metconazole, tebuconazole or prothioconazole.

Preferably, the mass ratio of the murrayone to the metconazole is 1-5:20-1.

Preferably, the mass ratio of the murrayone to the tebuconazole is 1-40:15-1.

Preferably, the mass ratio of the murrayone to the prothioconazole is 1-20:8-1.

A second object of the present invention is to provide a bactericide comprising the bactericidal composition and other auxiliary components.

The third object of the invention is to provide the application of the bactericidal composition in preventing and controlling wheat fungal diseases.

The fourth object of the invention is to provide the application of the bactericidal composition in preventing and treating wheat powdery mildew.

A fifth object of the present invention is to provide the use of the bactericidal composition in increasing thousand kernel weight of wheat.

Compared with the prior art, the invention has the following beneficial effects:

(1) When the murrayone and other effective components in the bactericidal composition are compounded, the bactericidal composition has a synergistic effect on pathogenic bacteria of wheat powdery mildew within a certain mass ratio range, can improve the control effect on the wheat powdery mildew, reduces the influence of the wheat powdery mildew on thousand seed weight of wheat, and further is beneficial to improving the thousand seed weight of the wheat.

(2) When the murrayone and other effective components in the bactericidal composition are compounded, the development of drug resistance of pathogenic bacteria can be effectively delayed, so that the drug resistance risk brought by the application of a single effective component can be reduced.

(3) The bactericidal composition can reduce the application amount of active ingredients and the control cost, and can provide support for developing novel medicaments.

Detailed Description

The invention will be better understood from the following examples. However, it will be readily appreciated by those skilled in the art that the description of the embodiments is provided for illustration only and should not limit the invention as described in detail in the claims.

Examples: indoor biological activity test of murrayone after compounding

1. Test crop: the 'Zhengmai 9023' wheat seeds are cultivated in a pot to 2-3 true leaf periods, and 10 plants are numbered for standby.

2. Test agent

98% Murrayone original drug (Shanghai Yuan Ye Biotechnology Co., ltd.)

95% metconazole crude drug (Shanghai Heteng Fine chemical Co., ltd.)

97% tebuconazole original drug (Shenyang scientific chemical Co., ltd.)

95% prothioconazole (Ningxia Yifan biotechnology Co., ltd.)

3. Test method (refer to NY/T1156.4-2008 "pesticide indoor biological assay test criterion Bactericide part 4: test potted method for preventing and treating wheat powdery mildew

3.1 preparation of medicament: dissolving the raw material with dimethyl sulfoxide, diluting with 0.1% Tween-80 solution, respectively preparing single-dose mother solution, setting multiple groups of ratios, and setting 5 series mass concentration gradients for each single dose and each group of ratio mixture;

3.2 inoculation treatment: uniformly shaking off fresh spores of powdery mildew (Blumeria graminis f.sp.tritici) generated in 24h on diseased wheat leaves, and inoculating the spores on wheat seedlings to be tested;

3.3 treatment with pharmaceutical agent: after 24 hours, the liquid medicine is evenly sprayed on the leaf surfaces of the wheat seedlings to be tested until the wheat seedlings are totally wet, each treatment basin is provided with 3 basins, and the treatment of the water solution containing only 0.1 percent of Tween-80 is used as a blank control; after natural air drying, the mixture is moved to a thermostatic chamber, and the mixture is irradiated at the temperature of 22 ℃ with light: dark = 16h: culturing was continued for 8 hours.

3.4 investigation and calculation: when the leaf rate of the blank control reaches more than 80%, the disease conditions of each treatment are investigated according to the grading standard of the table 1. And calculating the disease index and the prevention and treatment effect of each treatment according to the investigation data. Wherein, the grading standard is shown in Table 1.

TABLE 1 disease grade assessment criteria

3.5 data analysis: performing data statistical analysis by adopting DPS software, and performing linear regression with the disinfectant concentration logarithmic value x and the corresponding control efficiency numerical value y to obtain a virulence regression equation and virulence EC of the medicament on target bacteria ₅₀ Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.

3.6. Drug efficacy evaluation: the synergy of the agents was evaluated based on the calculated co-toxicity coefficients, CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 2-4.

Table 2 results of indoor biological Activity test of Murrayone and metconazole against wheat powdery mildew

As can be seen from table 2, at 1-5: within the mass ratio of 20-1, the common toxicity coefficient of the murrayone and the metconazole to the wheat powdery mildew is more than 120, and the synergistic effect is shown.

Table 3 results of indoor biological Activity test of Murrayone and Tebuconazole Complex against wheat powdery mildew

As can be seen from Table 3, at 1-40: within the mass ratio of 15-1, the common toxicity coefficient of the murrayone and the tebuconazole to the wheat powdery mildew is more than 120, and the synergistic effect is shown.

Table 4 results of indoor biological Activity test of Murrayone and Prothiabendazole on wheat powdery mildew

As can be seen from Table 4, at 1-20: in the mass ratio of 8-1, the common toxicity coefficient of the murrayone and the prothioconazole to the wheat powdery mildew is more than 120, and the synergistic effect is shown.

In conclusion, when the murrayone is compounded with metconazole, tebuconazole or prothioconazole in the bactericidal composition disclosed by the invention, the bactericidal composition has a synergistic effect on pathogenic bacteria of wheat powdery mildew within a certain mass ratio range, can improve the control effect on the wheat powdery mildew, reduces the influence of the wheat powdery mildew on thousand seed weight of wheat, and further is beneficial to improving the thousand seed weight of the wheat.

The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims

1. The sterilization composition is characterized in that the effective components of the sterilization composition are compounded by murrayone and metconazole, tebuconazole or prothioconazole.

2. The bactericidal composition of claim 1, wherein the mass ratio of murrayone to metconazole is 1-5:20-1.

3. The bactericidal composition of claim 1, wherein the mass ratio of murrayone to tebuconazole is 1-40:15-1.

4. The bactericidal composition of claim 1, wherein the mass ratio of murrayone to prothioconazole is 1-20:8-1.

5. A biocide comprising the biocide composition of any one of claims 1 to 4 and other auxiliary ingredients.

6. Use of the fungicidal composition as claimed in any one of claims 1 to 4 for controlling fungal diseases of wheat.

7. Use of the bactericidal composition as claimed in any one of claims 1 to 4 for controlling powdery mildew of wheat.

8. Use of the bactericidal composition of any of claims 1-4 for increasing thousand kernel weight of wheat.