CN116196243B

CN116196243B - Preparation method of plant oil-control and anti-dandruff composition

Info

Publication number: CN116196243B
Application number: CN202310057284.7A
Authority: CN
Inventors: 赵如意; 李凤姣
Original assignee: Guangzhou Baoyi Biotechnology Co ltd
Current assignee: Guangzhou Baoyi Biotechnology Co ltd
Priority date: 2023-01-17
Filing date: 2023-01-17
Publication date: 2023-09-15
Anticipated expiration: 2043-01-17
Also published as: CN116196243A

Abstract

The application relates to the field of plant extraction, and particularly discloses a plant oil-control and dandruff-removal composition and a preparation method thereof. The plant oil control and dandruff removal composition is prepared from the following raw materials: root of red sage; cortex Cinnamomi; eucalyptus globulus leaves; camphor tree leaves; radix Arnebiae; bark of magnolia bark; flos Caryophylli; centella asiatica leaves; aloe vera leaf. The preparation method of the plant oil-control and anti-dandruff composition comprises the following steps: mixing and cleaning the raw materials, crushing, extracting with alcohol, extracting with water, filtering, and concentrating to obtain the plant oil-controlling and dandruff-removing composition. The plant oil-control and anti-dandruff composition prepared by the application has long-lasting oil-control and anti-dandruff effects, and is not easy to cause irritation to scalp.

Description

Preparation method of plant oil-control and anti-dandruff composition

Technical Field

The application relates to the field of plant extraction, in particular to a plant oil-control and dandruff-removal composition and a preparation method thereof.

Background

Hair is hair growing on the head and is mainly used for protecting the head. Although hair is not an organ, hair plays an extremely important role in the appearance of people, so that hair care is more and more important, and especially hair oil control and dandruff removal are more and more interesting.

Bacteria on the scalp are the root cause of dandruff oil, mainly malassezia, and if malassezia is not removed cleanly, the problem of dandruff oil is always bad. Therefore, most of the current hair care products for shampoo are usually added with a large amount of antibacterial and bactericidal agents for better oil control and dandruff removal, and the purpose is to more completely kill malassezia. However, the antibacterial bactericide is easy to cause irritation to the scalp while killing malassezia of the scalp, and even causes inflammation of the scalp and serious alopecia. Thus, there is still room for improvement.

Disclosure of Invention

In order to better promote the sterilization and bacteriostasis effects of the washing and protecting product on malassezia, and further promote the mildness of the washing and protecting product, so that the oil control and dandruff removal effects of the washing and protecting product are longer lasting and long-acting, the application provides the plant oil control and dandruff removal composition and a preparation method thereof.

In a first aspect, the application provides a plant oil control and dandruff removal composition, which adopts the following technical scheme:

the plant oil control and dandruff removal composition comprises the following raw materials in parts by weight:

4-7 parts of root of red-rooted salvia;

1-3 parts of cinnamon bark;

1-3 parts of eucalyptus globulus leaves;

3-7 parts of camphor tree leaves;

1-3 parts of lithospermum root;

18-23 parts of magnolia bark;

3-6 parts of flos caryophylli;

2-5 parts of centella asiatica leaves;

3-6 parts of aloe vera leaf.

The composition is prepared by adopting the raw materials in a synergistic way, is favorable for better combining the effect of killing malassezia with the mild effect, and has the effects of controlling oil and removing dandruff for a longer time and a long time, and meanwhile, is favorable for better recuperating and nourishing the scalp.

In a second aspect, the application provides a preparation method of a plant oil-control and anti-dandruff composition, which adopts the following technical scheme: a preparation method of a plant oil-control and anti-dandruff composition is characterized by comprising the following steps: the method comprises the following steps:

step 1, taking root of red-rooted salvia, bark of cinnamon, camphor tree leaves, radix arnebiae, bark of magnolia officinalis, flos caryophylli and centella asiatica leaves, cleaning, drying and crushing;

step 2, taking aloe vera leaves, peeling, juicing, filtering, and taking filtrate to obtain aloe juice for later use;

step 3, adding the medicinal material crushed in the step 1 into extraction equipment, adding ethanol solution with the concentration of 75-80% which is 4.5-5.5 times of the mass of the medicinal material, stirring, heating, raising the temperature to 80-85 ℃, preserving heat, continuing stirring for 100-120min, and discharging the solution to obtain an ethanol extract for later use;

step 4, continuously adding water which is 3-3.5 times of the mass of the medicinal materials into the medicinal materials, stirring, starting ultrasonic waves, heating, raising the temperature to 80-85 ℃ within 10-15min, closing the ultrasonic waves, preserving heat for 90-100min, and discharging the solution to obtain a water extraction liquid for later use;

step 5, filtering the alcohol extraction liquid, taking filtrate, and concentrating the filtrate to 1-1.5 times of the mass of the medicinal material raw materials to obtain alcohol extraction concentrated liquid; filtering the water extraction liquid, taking filtrate, and concentrating the filtrate to 1-1.5 times of the mass of the raw materials to obtain water extraction concentrate;

step 6, mixing the alcohol extraction concentrated solution and the water extraction concentrated solution to form a mixed solution, adding polysorbate-80, uniformly stirring, and standing for 20-25h;

wherein the addition mass of the polysorbate-80 is 0.1-0.4% of the mass of the mixed solution;

step 7, extracting the supernatant after standing, uniformly mixing with the aloe juice prepared in the step 2, homogenizing for 1-3min, and stirring for 25-30min to obtain an intermediate product;

step 8, filtering the intermediate product, taking filtrate, adding preservative into the filtrate, and uniformly stirring to obtain the plant oil control and dandruff removal composition;

wherein the addition mass of the preservative is 0.8-1.8% of the mass of the filtrate.

The composition is prepared from the root of red-rooted salvia, the bark of cinnamon, the leaves of eucalyptus globulus, the leaves of camphor, the root of lithospermum, the bark of magnolia officinalis, flos caryophyllata, the leaves of centella asiatica and the leaves of aloe vera by synergistic compounding as raw materials and extracting under specific temperature and time conditions, so that the extracted composition has excellent effect of killing malassezia, and meanwhile, the composition is extracted from natural traditional Chinese medicinal materials, so that the skin is not easy to be stimulated and damaged, the sterilizing and antibacterial effects of the composition are not easy to be influenced due to the fact that the irritation is reduced, the composition can have the effects of killing malassezia and being mild and non-irritating, the effects of controlling oil and removing dandruff are improved better, and the scalp is also better nourished, and skin microecological unbalance and other hair problems are not easy to be caused due to oversterilization.

Meanwhile, the extraction is carried out in a mode of combining alcohol extraction under a specific temperature condition and water extraction under a specific temperature condition, so that effective components in medicinal materials are better extracted, and the effective components of the components are better compounded in a synergistic way, so that the composition has better effect of killing malassezia and is not easy to stimulate the scalp, and the composition can better have the effects of controlling oil and removing dandruff and is mild.

Preferably, in the step 4, water and ultrasonic treatment are added, and after heat preservation is carried out for 90-100min, the temperature is reduced to 30-50 ℃, compound enzyme is added, enzymolysis treatment is carried out for 20-30min, and the solution is discharged, so as to obtain water extraction liquid;

wherein the addition amount of the complex enzyme is 0.5-1% of the mass of water, and the complex enzyme is prepared from cellulase, papain, beta-glucanase and tannase according to a ratio of 5:0.8-1: 0.4-0.5:0.2-0.3.

Preferably, the enzyme activity of the cellulase is 10 ten thousand U/g, the enzyme activity of the papain is 20 ten thousand U/g, the enzyme activity of the beta-glucanase is 5 ten thousand U/g, and the enzyme activity of the tannase is 10 ten thousand U/g.

The enzyme compounded in a specific proportion is added to carry out enzymolysis on the medicinal materials in the water extraction process, so that effective components in the medicinal materials can be better extracted, meanwhile, the extracted composition can better nourish the scalp, the scalp is better in a balanced state, bacteria are not easy to reproduce again after the scalp is stopped, the scalp has a better self-regulating function, and the oil control and dandruff removal effects of the composition are longer lasting and long-acting.

Preferably, in the step 4, after adding the complex enzyme, a pH adjustor is added to adjust the pH to 5-7, and the temperature is controlled to 50-55 ℃.

The pH value and the temperature condition in the enzymolysis process are controlled, so that the compound enzyme can better cooperate and act better, the effective components in the medicinal material raw materials can be better extracted, the effects of the composition on nursing and nourishing the scalp can be better improved, the scalp has a better self-regulating function, and the oil control and dandruff removal effects of the composition are longer lasting and long-lasting.

Preferably, the pH adjuster is a volatizable material.

By adopting the volatilizable substances as the pH regulator, the pH regulator is beneficial to removing, and impurities are not easy to bring to the composition, so that the effect of the composition is not easy to be influenced.

Preferably, in the step 4, after adding the complex enzyme, the enzyme is treated by using a pulsed electric field for 10-15min, and then the enzymolysis treatment is performed.

The pulse electric field treatment is firstly utilized and then the enzymolysis treatment is carried out, so that the activity of the compound enzyme is better improved, the compound enzyme is better cooperated and compounded and plays the role of enzymolysis, the effective components in the medicinal material raw materials are better extracted, and the effects of nursing and nourishing the scalp of the composition are better.

Preferably, in the step 4, the pulse voltage of the pulse electric field treatment is controlled to be 2-5kV, the pulse frequency is 350-400Hz, and the pulse width is 10-15 mu s.

The treatment conditions of the pulsed electric field treatment are controlled, so that the activity of the complex enzyme is better promoted, the complex enzyme is better cooperated and compounded, the enzymolysis effect is achieved, the effective components in the medicinal material raw materials are better extracted, and the effects of nursing and nourishing the scalp of the composition are better.

Preferably, in the step 1, the raw materials of the medicinal materials are immersed in water for 30-40min, then cleaned by ultrasonic waves for 5-10min and dried.

The medicinal material raw materials are soaked in water and then washed, so that the medicinal material raw materials are easier to extract in the subsequent extraction process, and the extraction effect of the medicinal material raw materials is improved better; meanwhile, the ultrasonic wave is matched for cleaning, so that the cleaning effect and the cleaning efficiency of the cleaned medicinal material raw materials are improved better, impurities are not easy to be contained in the medicinal material raw materials, the extracted composition is not easy to contain impurities, and the effect of the composition is improved further.

Preferably, in the step 1, the raw materials are crushed into 1-2mm particles after being dried.

The particle size of the broken particles of the medicinal material raw material is controlled, so that the medicinal material raw material is better extracted in the subsequent extraction process, and the extraction efficiency and the extraction effect of the medicinal material raw material are improved.

In summary, the application has the following beneficial effects:

1. the composition is prepared by adopting the raw materials in a synergistic way, is favorable for better combining the effect of killing malassezia with the mild effect, and has the effects of controlling oil and removing dandruff for a longer time and a long time, and meanwhile, is favorable for better recuperating and nourishing the scalp.

2. The composition is prepared from the root of red-rooted salvia, the bark of cinnamon, the leaves of eucalyptus globulus, the leaves of camphor, the root of lithospermum, the bark of magnolia officinalis, flos caryophyllata, the leaves of centella asiatica and the leaves of aloe vera by synergistic compounding as raw materials and extracting under specific temperature and time conditions, so that the extracted composition has excellent effect of killing malassezia, and meanwhile, the composition is extracted from natural traditional Chinese medicinal materials, so that the skin is not easy to be stimulated and damaged, the sterilizing and antibacterial effects of the composition are not easy to be influenced due to the fact that the irritation is reduced, the composition can have the effects of killing malassezia and being mild and non-irritating, the effects of controlling oil and removing dandruff are improved better, and the scalp is also better nourished, and skin microecological unbalance and other hair problems are not easy to be caused due to oversterilization.

3. The extraction is carried out in a mode of combining alcohol extraction under a specific temperature condition and water extraction under a specific temperature condition, so that effective components in medicinal materials are better extracted, the effective components of the components are better compounded in a synergistic way, the effect of killing malassezia of the composition is better, meanwhile, the scalp is not easy to stimulate, and the composition can better have the effects of controlling oil and removing dandruff and is mild.

Detailed Description

The present application will be described in further detail with reference to examples.

Example 1

The embodiment of the application discloses a plant oil control and dandruff removal composition which is prepared from the following raw materials in mass:

4kg of root of red-rooted salvia; 1kg of cinnamon bark; 1kg of eucalyptus globulus leaves; 3kg of camphor tree leaves; 1kg of lithospermum root; 18kg of magnolia bark; 3kg of flos caryophylli; 2kg of centella asiatica leaves; aloe vera leaf 3kg.

The embodiment of the application also discloses a preparation method of the plant oil-control and anti-dandruff composition, which comprises the following steps:

step 1, weighing root of red-rooted salvia, bark of cinnamon, eucalyptus globulus leaf, camphor tree leaf, radix arnebiae root, bark of magnolia officinalis, flos caryophylli and centella asiatica according to the above-mentioned quality, soaking the weighed raw materials of medicinal materials in deionized water for 30min, cleaning for 10min by using ultrasonic waves, controlling the frequency of the ultrasonic waves to be 30kHz, fishing up the raw materials of medicinal materials after cleaning, draining, putting into a baking oven to be dried until the water content is below 10%, and adding the dried raw materials of medicinal materials into a crusher to be crushed into particles with the particle size of 1-2 mm.

And 2, weighing aloe vera leaves according to the mass, peeling, putting into a juicer for juicing, filtering, and taking filtrate to obtain aloe juice for later use.

And 3, adding the medicinal material raw material particles obtained in the step 1 into an extraction tank, adding an ethanol solution with the mass of 4.5 times of the mass of the medicinal material raw material and the concentration of 80%, stirring at the stirring speed of 30r/min, heating, raising the temperature to 80 ℃, preserving heat, continuing stirring for 120min, discharging the solution, obtaining an ethanol extract, and reducing the temperature to below 40 ℃ by using a heat exchanger for later use.

And 4, continuously adding water with the mass which is 3 times that of the medicinal material raw materials into the medicinal material raw materials, stirring at the rotating speed of 20r/min, simultaneously starting ultrasonic waves, controlling the frequency of the ultrasonic waves to be 20kHz, heating, raising the temperature to 80 ℃ within 10min, closing the ultrasonic waves, preserving the heat for 100min, and discharging the solution to obtain an aqueous extract for later use.

Step 5, adding the ethanol extract into an active carbon filter, filtering, taking filtrate, adding the filtrate into a membrane concentration system, concentrating at normal temperature, controlling the concentration pressure to be 2.5MPa, and concentrating to 1 time of the mass of the raw materials of the medicinal materials to obtain an ethanol extract concentrate; adding activated carbon filter into the water extraction extract, filtering, collecting filtrate, adding the filtrate into membrane concentration system, concentrating at normal temperature, controlling concentration pressure to 3MPa, and concentrating to 1 times of the mass of the raw materials to obtain water extraction concentrate.

And 6, mixing the alcohol extraction concentrated solution and the water extraction concentrated solution to form a mixed solution, pumping the mixed solution into an emulsifying pot, adding polysorbate-80 with the mass accounting for 0.1% of the mass of the mixed solution, uniformly stirring at a rotating speed of 30r/min, and standing for 20h.

And 7, extracting the supernatant after standing, uniformly mixing with the aloe juice prepared in the step 2, homogenizing for 1min, controlling the homogenizing rotating speed to be 2000r/min, reducing the stirring rotating speed to 20r/min after homogenizing, and continuing stirring for 30min to obtain an intermediate product.

And 8, filtering the intermediate product again by using a precise filter, taking filtrate, adding preservative with the mass accounting for 0.8% of the mass of the filtrate into the filtrate, and uniformly stirring to obtain the plant oil control and anti-dandruff composition.

The preservative is prepared by mixing p-hydroxyacetophenone and 1, 2-hexanediol according to the mass ratio of 1:2, mixing the p-hydroxyacetophenone and the 1, 2-hexanediol, raising the temperature to 80 ℃ until the two substances are completely dissolved and completely mixed uniformly, and then adding the mixture into filtrate.

Example 2

The difference from example 1 is that:

the plant oil control and dandruff removal composition has different raw material addition amounts, and specifically comprises the following steps:

7kg of root of red-rooted salvia; 3kg of cinnamon bark; 3kg of eucalyptus globulus leaves; 7kg of camphor tree leaves; 3kg of lithospermum root; 23kg of magnolia bark; 6kg of flos caryophylli; 5kg of centella asiatica leaves; 6kg of aloe vera leaf.

The technological parameters of each step in the preparation method are different, and the preparation method specifically comprises the following steps:

in the step 1, the raw materials of the medicinal materials are soaked in deionized water for 40min, and then are cleaned by ultrasonic waves for 5min.

In the step 3, the concentration of the added ethanol solution is 75%, and the added mass of the ethanol solution is 5.5 times of the mass of the medicinal materials; heating to 85deg.C, and maintaining for 100min.

In the step 4, the mass of the added water is 3.5 times of the mass of the raw materials of the medicinal materials, the temperature is increased to 85 ℃ within 15min after the ultrasonic wave is started, and the heat preservation time is 90min after the ultrasonic wave is closed.

In the step 5, the alcohol extraction liquid is concentrated to 1.5 times of the mass of the raw materials of the medicinal materials, so as to obtain alcohol extraction concentrated liquid; concentrating the water extraction extract to 1.5 times of the raw materials to obtain water extraction concentrate.

In the step 6, the added mass of the polysorbate-80 is 0.4% of the mass of the mixed solution, and the mixture is stirred uniformly and then is kept stand for 25 hours.

In the step 7, the homogenization time is 3min and the stirring time is 25min.

In the step 8, the addition mass of the preservative is 1.8% of the mass of the filtrate.

Example 3

The difference from example 1 is that:

5.5kg of root of red-rooted salvia; 2kg of cinnamon bark; 2kg of eucalyptus globulus leaves; 5kg of camphor tree leaves; 2kg of lithospermum root; 20kg of magnolia bark; 5kg of flos caryophylli; 3.5kg of centella asiatica leaves; 5kg of aloe vera leaf.

in the step 1, the raw materials of the medicinal materials are soaked in deionized water for 35min, and then are cleaned by ultrasonic waves for 8min.

In the step 3, the concentration of the added ethanol solution is 78%, and the added mass of the ethanol solution is 5 times of the mass of the medicinal materials; heating to 83 deg.C, and maintaining for 110min.

In the step 4, the mass of the added water is 3 times of that of the raw materials of the medicinal materials, the temperature is increased to 82 ℃ within 13min after the ultrasonic wave is started, and the heat preservation time is 95min after the ultrasonic wave is closed.

In the step 5, the alcohol extraction liquid is concentrated to 1 time of the mass of the raw materials of the medicinal materials, so as to obtain alcohol extraction concentrated liquid; concentrating the water extraction extract to 1 time of the mass of the raw materials to obtain water extraction concentrate.

In the step 6, the added mass of the polysorbate-80 is 0.3% of the mass of the mixed solution, and the mixture is stirred uniformly and then is kept stand for 22 hours.

In the step 7, the homogenization time is 2min, and the stirring time is 28min.

In the step 8, the addition mass of the preservative is 1.2% of the mass of the filtrate.

Example 4

The difference from example 3 is that:

the specific operation of the step 4 is as follows:

continuously adding water with the mass which is 3 times that of the medicinal materials into the medicinal materials, stirring at the rotating speed of 20r/min, simultaneously starting ultrasonic waves, controlling the frequency of the ultrasonic waves to be 20kHz, heating, raising the temperature to 82 ℃ within 13min, closing the ultrasonic waves, preserving heat for 95min, lowering the temperature to 30 ℃, adding complex enzyme, adding sodium bicarbonate to adjust the pH to 5, raising the temperature to 50 ℃, preserving heat and carrying out enzymolysis for 30min, discharging the solution, and obtaining water extraction liquid for standby.

Wherein, the added mass of the compound enzyme is 0.5% of the mass of water, and the compound enzyme is prepared by uniformly mixing cellulase, papain, beta-glucanase and tannase according to the mass ratio of 5:0.8:0.4:0.2.

In this example, the cellulase was purchased from Hubei Chu Biotechnology Co., ltd, CAS number 9012-54-8, and enzyme activity of 10 ten thousand U/g;

papain was purchased from Waters biotechnology Co., ltd. In Lanzhou, with an enzyme activity of 20U/g;

beta-glucanase is purchased from Lifan chemical industry Co., ltd. Shandong, the CAS number is 9025-70-1, and the enzyme activity is 5 ten thousand U/g;

tannase is purchased from Shandong Siyang Biotechnology Inc. and has an enzyme activity of 10U/g.

Example 5

The difference from example 4 is that:

in the step 4, after heat preservation, the temperature is reduced to 50 ℃, complex enzyme is added, sodium bicarbonate is added to adjust the pH to 7, the temperature is increased to 55 ℃, heat preservation and enzymolysis are carried out for 20min, and the solution is discharged, so as to obtain water extraction liquid.

The added mass of the compound enzyme is 1% of the mass of water, and the compound enzyme is prepared by uniformly mixing cellulase, papain, beta-glucanase and tannase according to the mass ratio of 5:1:0.5:0.3.

Example 6

The difference from example 4 is that: the cellulase was replaced with an equal amount of hemicellulase.

In this example, hemicellulases were purchased from Nanjing Tongyi biotechnology Co., ltd, and the enzyme activity was 10 ten thousand U/g.

Example 7

The difference from example 4 is that: papain was replaced with an equal amount of bromelain.

In this example, bromelain was purchased from ataxia hao-hong biotechnology limited and the enzyme activity was 20 ten thousand U/g.

Example 8

The difference from example 4 is that: the beta-glucanase was replaced with an equal amount of alpha-amylase.

In this example, the alpha-amylase was purchased from Shanghai ze Biotechnology Inc., under the trade designation ZY60662RE and the enzyme activity was 5 ten thousand U/g.

Example 9

The difference from example 4 is that: the tannase is replaced by the same amount of pectase.

In this example, pectase was purchased from Seaman harvest Biotechnology Co., ltd, and the enzyme activity was 10 ten thousand U/g.

Example 10

The difference from example 4 is that:

the complex enzyme is prepared by uniformly mixing cellulase, papain, beta-glucanase and tannase according to the mass ratio of 5:0.5:2:0.1.

Example 11

The difference from example 4 is that:

the complex enzyme is prepared by uniformly mixing cellulase, papain, beta-glucanase and tannase according to the mass ratio of 5:1.5:1:0.5.

Example 12

The difference from example 4 is that:

the enzymolysis conditions in the step 4 are different, and the specific steps are as follows:

after heat preservation for 95min, the temperature is reduced to 25 ℃, compound enzyme is added, sodium bicarbonate is added to adjust the pH to 8, the temperature is increased to 60 ℃, heat preservation and enzymolysis are carried out for 35min, and the solution is discharged to obtain water extraction liquid for standby.

Example 13

The difference from example 4 is that:

the specific operation of the step 4 is as follows:

continuously adding water with the mass which is 3 times that of the medicinal materials into the medicinal materials, stirring at the rotating speed of 20r/min, simultaneously starting ultrasonic waves, controlling the frequency of the ultrasonic waves to be 20kHz, heating, raising the temperature to 82 ℃ within 13min, closing the ultrasonic waves, preserving heat for 95min, lowering the temperature to 30 ℃, adding compound enzyme, treating for 15min by using a pulse electric field, controlling the pulse voltage to be 2kV, controlling the pulse frequency to be 350Hz and controlling the pulse width to be 10 mu s. After the pulsed electric field treatment is completed, adding sodium bicarbonate to adjust the pH to 5, raising the temperature to 50 ℃, preserving the heat and carrying out enzymolysis for 30min, and discharging the solution to obtain an aqueous extract for later use.

Example 14

The difference from example 13 is that:

the process parameters of the pulsed electric field treatment are different, and the method concretely comprises the following steps:

the treatment time of the pulsed electric field was 10min, and the pulse voltage was controlled to 5kV, the pulse frequency was 400Hz, and the pulse width was 15. Mu.s.

Example 15

The difference from example 13 is that:

the treatment time of the pulsed electric field was 20min, and the pulse voltage was controlled to 8kV, the pulse frequency was 450Hz, and the pulse width was 18. Mu.s.

Comparative example 1

The difference from example 3 is that: in step 1, the root of Salvia Miltiorrhiza is replaced by equal amount of rhizoma Ligustici Chuanxiong.

Comparative example 2

The difference from example 3 is that: in step 1, the raw material cinnamon bark is replaced with equal amount of nutmeg.

Comparative example 3

The difference from example 3 is that: in step 1, the raw material eucalyptus globulus leaves are replaced by the same amount of rhizoma anemarrhenae roots.

Comparative example 4

The difference from example 3 is that: in the step 1, the camphor tree leaves are replaced by the same amount of sandalwood.

Comparative example 5

The difference from example 3 is that: in the step 1, the raw material radix arnebiae is replaced by the same amount of dandelion.

Comparative example 6

The difference from example 3 is that: in step 1, the bark of Magnolia officinalis is replaced with equivalent bark of white willow.

Comparative example 7

The difference from example 3 is that: in the step 1, the raw material flos Caryophylli is replaced by equivalent flos Jasmini sambac.

Comparative example 8

The difference from example 3 is that: in step 1, the same amount of evening primrose seeds are used for replacing the raw material centella asiatica leaves.

Comparative example 9

The difference from example 3 is that: in step 1, the raw material aloe vera leaf is replaced with an equal amount of cactus.

Experiment 1 Malachite bacteria sterilizing Effect test

1.1 test materials

Malassezia (pityrosporum): number ATCC 44344; GDM number: GIM2.181.

Culture medium: 5.0g of peptone; 3.0g of yeast extract (dried); 3.0g of malt extract; glucose 10.0g; 10.0g of olive oil; 20.0g of agar; distilled water 1L. The pH was adjusted to 6.0.

1.2 test methods

Step 1, activating strains, which comprises the following steps:

under the aseptic condition, 0.2ml of aseptic water is used for dissolving malassezia furfur freeze-dried powder, then the malassezia furfur freeze-dried powder is inoculated on 2 inclined planes, the culture is carried out for 48 hours at the temperature of 30 ℃, and the strain is preserved in the low-temperature environment of 5 ℃ to avoid polluting the environment.

Step 2, preparing a flat plate, which comprises the following steps:

preparing activated malassezia furfur into a product with a particle size of more than 10 ⁷ cfu/mL of the bacterial suspension, and 0.3mL of the bacterial suspension is smeared on a 12cm culture medium plate. And (3) placing the bacteria-containing flat plate for 10min, opening a flat plate cover in a sterile ultra-clean workbench, and drying the flat plate for 20min by using sterile wind.

And 3, preparing a sample sheet, wherein the sample sheet is prepared by the following steps:

taking Xinhua No. one qualitative filter paper, beating into a circular filter disc with the diameter of 5cm by using a puncher, sterilizing by using pressure steam, drying, taking a plurality of sterilized and dried sample pieces, respectively dipping a small amount of to-be-tested sample on the sample pieces by using sterile forceps, and uniformly smearing the to-be-tested sample on the sample pieces.

Wherein, the test sample corresponds to the plant oil control and dandruff removal composition prepared in the above examples and comparative examples respectively; in addition, the measured sample is the glycin dissolved in absolute ethyl alcohol with the concentration of 0.1g/mL, and the measured sample is used as a positive control group; as a negative control group, no test sample was added. And 5 pieces of corresponding test pieces of each test piece are repeatedly manufactured.

And 4, determining the antibacterial effect, wherein the antibacterial effect is determined by the following steps:

and (3) taking 5 plates, and respectively attaching any one of the repeated test pieces prepared in the step (3) to each plate. After the adhesion, the sample piece was lightly pressed with sterile forceps to be tightly attached to the surface of the flat plate, the flat plate was covered, the flat plate was placed in a constant temperature incubator at 32℃for 7 days for inverted culture, the diameter (mm) of the inhibition zone was measured with a vernier caliper, and the average value of 3 sets of measurement values was calculated and recorded. In addition, the antibacterial ratio (%) of each example and comparative example was calculated,

the diameter of the inhibition zone is more than or equal to 8mm, and the inhibition zone is visible to the naked eye, so that the antibacterial effect is proved; the larger the antibacterial rate is, the more obvious the antibacterial effect is proved.

The test data of experiment 1 are detailed in table 1.

TABLE 1 Malachite bacteria sterilizing Effect test detection data

Experiment 2

The plant oil-controlling and chip-removing compositions prepared in the above examples and comparative examples were diluted with water to a concentration of 10%, and filter paper sheets soaked with the plant oil-controlling and chip-removing compositions prepared in the above examples and comparative examples were placed in a plaque tester, respectively, with normal wells as blank controls.

30 volunteers were randomly selected, and aged 25-45 years, and were not limited to men and women. The patch testers and normal blank controls corresponding to the plant oil control and dandruff removal composition samples prepared in the examples and the comparative examples are respectively stuck on the curved side of the forearm of a subject, and are uniformly stuck on the skin by using palm light pressure for 24 hours, the interval is 30 minutes after the patch testers are removed, and skin reaction is observed after the indentations disappear. And recording the number of people with no red swelling and pruritus, the number of people with pruritus or slight red swelling, the number of people with pure erythema and pruritus the number of people with redness and swelling, pimples and small blisters and the number of people with redness and swelling, blisters and necrosis.

The test data of experiment 2 are detailed in table 2.

TABLE 2

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Experiment 3

The shampoo is prepared from the vegetable oil-control and anti-dandruff composition prepared in the above examples and comparative examples according to the following components by mass: 2kg of a plant oil control and dandruff removal composition; 8kg of ammonium lauryl sulfate; 10kg of laureth ammonium sulfate; 2kg of cocamide MEA; 5kg of cocamidopropyl betaine; 3kg of emulsified silicone oil; guar hydroxypropyl trimethylammonium chloride 0.2kg; citric acid 0.05kg; ammonium chloride 0.5kg; panthenol 0.5kg; ethylene glycol distearate 2kg; 0.3kg of essence; 0.6kg of phenoxyethanol; 0.2kg of methyl paraben; 65.65kg of deionized water.

Wherein the silicone emulsion is available from International trade company of bolt works, guangzhou under the model 3560, namely sodium dimethyl silicone (and) laureth-23 (and) laureth sulfate.

The preparation method of the shampoo comprises the following steps:

weighing the components according to the mass, firstly mixing guar hydroxypropyl trimethyl ammonium chloride and deionized water, uniformly stirring at a rotating speed of 30r/min, then adding citric acid, glycol distearate, ammonium lauryl sulfate, ammonium laureth sulfate, cocamide MEA and methylparaben, raising the temperature to 80 ℃, stirring at a rotating speed of 50r/min, reducing the temperature to 50 ℃ after uniformly stirring, then adding the plant oil control and anti-dandruff composition, emulsified silicone oil and cocamidopropyl betaine, continuously stirring uniformly, adding ammonium chloride dissolved by water, and uniformly stirring. Finally adding essence, phenoxyethanol and panthenol, continuously stirring for 20min, and standing until defoaming to obtain the shampoo containing the plant oil control and dandruff removal composition.

120 volunteers with serious dandruff problems and scalp oil problems are randomly selected, the ages are 25-45 years, men and women are not limited, the volunteers are randomly divided into 24 groups, 5 people in each group are respectively and correspondingly washed by commercially available shampoo which is used daily, after the hair is dried for 12 hours and 24 hours, the scalp oil and the dandruff are detected by using the scalp oil test paper after the hair is dried for 12 hours and the scalp oil test paper is dried for 24 hours, namely, the scalp oil test paper and the scalp oil test paper are respectively used for lightly pressing the head for 5 seconds, the scalp oil test paper and the scalp oil test paper are taken off, the black dot area ratio (%) of the scalp oil test paper is calculated, and the number of the dandruff particles (particles) on the scalp oil test paper is counted.

After the above test was completed, each group of volunteers was respectively and correspondingly used with shampoos prepared using the vegetable oil-controlling and anti-dandruff compositions prepared in the above examples and comparative examples, and was shampooed once every two days, continuously used for 60 days, and completed the last time, and after the hair was dried for 24 hours and 48 hours, the scalp oil and dandruff were detected by re-using the effect scalp test paper, i.e., the head was lightly pressed for 5 seconds by using the oil test paper and the dandruff test paper, respectively, the oil test paper and the dandruff test paper were removed, the black spot area ratio (%) of the oil test paper was calculated, and the number of dandruff particles (particles) on the dandruff test paper was counted.

After each group of volunteers used the above shampoo for 60 days and completed the above test, the shampoo containing the vegetable oil-controlling and anti-dandruff compositions prepared in the corresponding examples and comparative examples was stopped. In addition, according to the shampoo formula, water is used for replacing the vegetable oil control and dandruff removal composition in the shampoo to prepare a blank control shampoo, each group of volunteers continuously washes the hair for 30 days by using the blank control shampoo respectively, and washes the hair once every two days, and after the last time of hair washing is completed and the hair is dried for 24 hours and 48 hours, the scalp oil and dandruff are detected by using the effect scalp detection test paper, namely, the head is lightly pressed for 5 seconds by using the scalp oil test paper and the dandruff test paper respectively, the scalp oil test paper and the dandruff test paper are taken off, the black spot area ratio (%) of the scalp oil test paper is calculated, and the number of dandruff particles (particles) on the scalp dandruff test paper is counted.

The detection data of each group are counted and an average value is calculated, and the larger the black dot area ratio of the head oil test paper is, the more serious the head oil is proved to be; the smaller the number of the dandruff particles on the dandruff test paper, the better the anti-dandruff effect is proved. The test data of experiment 3 are shown in Table 3 and Table 4.

TABLE 3 Table 3

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TABLE 4 Table 4

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According to comparison of the data of examples 3 and comparative examples 1 to 4, 6 to 7 and 9 in tables 1 and 2, the materials having similar effects were used in comparative examples 1 to 4, 6 to 7 and 9 to replace the raw materials of root of red sage, bark of cinnamon, leaf of eucalyptus globulus, leaf of camphor tree, bark of magnolia officinalis, lilac flower and leaf of aloe vera, respectively, while comparative examples 1 to 4, 6 to 7 and 9 have better sterilizing effect on malassezia, but at the same time have great influence on the mildness of the plant oil-controlling and anti-dandruff composition, so that the plant oil-controlling and anti-dandruff composition is even liable to cause inflammation such as reddening, pimple, etc.

Meanwhile, according to the comparison of the data of example 3 with those of comparative examples 1 to 4, 6 to 7 and comparative example 9 in Table 3, the comparison of examples 1 to 4, 6 to 7 and comparative example 9 is also that after the shampoo containing the plant oil-controlling and anti-dandruff composition is deactivated, the dandruff and oil-yielding situation is rather serious, because the sterilizing performance of the plant oil-controlling and anti-dandruff composition is too strong, even the ecological balance of the scalp is easily disturbed, so that the shampoo containing the plant oil-controlling and anti-dandruff composition is more likely to grow malassezia after the shampoo is deactivated, and the dandruff and oil-yielding problem is more serious.

From the comparison of the data of examples 3 and 5 and 8 in tables 1 and 2, comparative examples 5 and 8 respectively use substances having similar effects instead of radix Arnebiae and herba Centellae, and although the mildness of comparative examples 5 and 8 is better than that of example 3, they also result in great influence on the sterilizing performance of comparative examples 5 and 8. As can be seen from a comparison of the data in table 3 for example 3 with the data for comparative example 5 and comparative example 8, the effect of controlling oil and chip is affected even by affecting the sterilizing effect of the plant oil and chip controlling composition for comparative example 5 and comparative example 8.

In summary, according to the comparison of the data of the embodiment 3 and the data of the comparative examples 1 to 9 in the tables 1,2 and 3, only by adopting the synergistic compounding of the root of red-rooted salvia, the bark of cinnamon, the leaf of eucalyptus globulus, the leaf of camphor tree, the root of lithospermum, the bark of magnolia officinalis, the flower of lilac, the leaf of centella asiatica and the leaf of aloe vera as the extraction raw materials, the plant oil-control and chip-removing composition can be made to have both sterilization performance and mildness, so that the plant oil-control and chip-removing composition has longer lasting oil-control and chip-removing effects, and is not easy to cause irritation and damage to the scalp to cause other inflammation problems.

According to the comparison of the data of examples 4-12 in Table 1 and Table 2, compared with example 4, examples 6-9 replace any one of the enzymes respectively, examples 10-11 change the dosage proportion of various enzymes in the complex enzyme, example 12 change the enzymolysis condition, and the sterilization effect of examples 4-5 is better than that of examples 6-12, and the mildness of examples 4-5 is similar to that of examples 6-12, namely, the synergistic combination of enzymes with specific proportion and specific activity is proved, and the enzymolysis is carried out under specific enzymolysis condition, so that the extraction of the effective components in the medicinal materials is facilitated to be better, and the sterilization effect of the plant oil-control and dandruff-removing composition is further improved, and meanwhile the mildness of the plant oil-control and dandruff-removing composition is not easy to be influenced.

Meanwhile, according to the comparison of the data of the examples 4-12 in the table 3, the oil control and dandruff removal effects of the examples 4-5 can be maintained to be in a state when the shampoo containing the oil control and dandruff removal composition is used after the shampoo containing the oil control and dandruff removal composition is stopped, and the oil control and dandruff removal effects of the examples 6-12 are restored to be in a state before use, which proves that only the synergistic combination of enzymes with specific proportion and specific activity is adopted in a specific proportion and enzymolysis is carried out under a specific enzymolysis condition, the effective components in the medicinal raw materials can be better extracted, the oil control and dandruff removal composition also has the scalp care effect, further the scalp has better self-regulating capability, the oil control and dandruff removal effect is longer, the use ratio of any one of the enzymes is changed, or the enzymolysis condition is changed, and the extract of the medicinal raw materials can not achieve the scalp care effect.

According to the data comparison between the examples 4 and 13-15 in tables 1,2 and 3, compared with the example 4, the example 13-15 is added with the enzyme, and the pulse electric field treatment is added, but the process parameters of the pulse electric field of the example 13-15 are different, but the sterilization effect of the example 13-14 is better than that of the example 4, and the oil control and chip removal effect after the shampoo containing the plant oil control and chip removal composition is stopped, but the sterilization effect of the example 15 is worse than that of the example 6-12, and the lasting long-acting property of the oil control and chip removal effect of the example 15 is also worse than that of the example 6-12 after the shampoo containing the plant oil control and chip removal composition is stopped, so that the enzyme activity is improved by the pulse electric field treatment enzyme with proper parameters, the active ingredients of the medicinal materials are easier to be extracted, and the extraction is more efficient, but if the process parameters of the pulse electric field are not proper, the enzyme activity is easier to be influenced, the enzymolysis is easier to be influenced, and the active ingredients of the medicinal materials are easier to be influenced, and the active ingredients are longer-acting and the active ingredients of the medicinal materials are influenced by the extraction and the active ingredients are influenced by the long-acting property of the plant oil control and chip removal composition.

The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.

Claims

1. A preparation method of a plant oil-control and anti-dandruff composition is characterized by comprising the following steps: the plant oil control and dandruff removal composition is prepared from the following raw materials in parts by mass:

4-7 parts of root of red-rooted salvia;

1-3 parts of cinnamon bark;

1-3 parts of eucalyptus globulus leaves;

3-7 parts of camphor tree leaves;

1-3 parts of lithospermum root;

18-23 parts of magnolia bark;

3-6 parts of flos caryophylli;

2-5 parts of centella asiatica leaves;

3-6 parts of aloe vera leaf;

the preparation method of the plant oil control and dandruff removal composition comprises the following steps:

step 1, weighing root of red-rooted salvia, bark of cinnamon, eucalyptus globulus leaf, camphor tree leaf, radix arnebiae, bark of magnolia officinalis, flos caryophylli and centella asiatica according to the parts by weight of the raw materials, cleaning, drying and crushing;

2. The method for preparing the plant oil-control and anti-dandruff composition according to claim 1, wherein the method comprises the following steps: in the step 4, water and ultrasonic treatment are added, after heat preservation is carried out for 90-100min, the temperature is reduced to 30-50 ℃, compound enzyme is added, enzymolysis treatment is carried out for 20-30min, and solution is discharged, thus obtaining water extraction liquid;

3. The method for preparing the plant oil-control and anti-dandruff composition according to claim 2, wherein the method comprises the following steps: the enzyme activity of the cellulase is 10 ten thousand U/g, the enzyme activity of the papain is 20 ten thousand U/g, the enzyme activity of the beta-glucanase is 5 ten thousand U/g, and the enzyme activity of the tannase is 10 ten thousand U/g.

4. A method for preparing a plant oil control and anti-dandruff composition according to any of claims 2-3, characterized in that: in the step 4, after the complex enzyme is added, a pH regulator is added to regulate the pH to 5-7, and the temperature is controlled to be 50-55 ℃.

5. The method for preparing the vegetable oil-control and anti-dandruff composition according to claim 4, wherein the method comprises the following steps: the pH regulator is a volatizable material.

6. A method for preparing a plant oil control and anti-dandruff composition according to any of claims 2-3, characterized in that: in the step 4, after the complex enzyme is added, the complex enzyme is treated for 10-15min by using a pulse electric field, and then enzymolysis treatment is carried out.

7. The method for preparing the vegetable oil-control and anti-dandruff composition according to claim 6, wherein the method comprises the following steps: in the step 4, the pulse voltage of the pulse electric field treatment is controlled to be 2-5kV, the pulse frequency is 350-400Hz, and the pulse width is 10-15 mu s.

8. A method for preparing a plant oil control and anti-dandruff composition according to any of claims 1-3, characterized in that: in the step 1, the raw materials of the medicinal materials are immersed in water, soaked for 30-40min, cleaned by ultrasonic waves for 5-10min and dried.

9. A method for preparing a plant oil control and anti-dandruff composition according to any of claims 1-3, characterized in that: in the step 1, the medicinal materials are dried and crushed into particles with the diameter of 1-2 mm.