CN113332201A

CN113332201A - Anti-hair loss shampoo and preparation method thereof

Info

Publication number: CN113332201A
Application number: CN202110640884.7A
Authority: CN
Inventors: 钟娟
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-06-08
Filing date: 2021-06-08
Publication date: 2021-09-03

Abstract

The invention discloses an anti-hair loss shampoo, which comprises the following raw materials: chitosan quaternary ammonium salt, laurylamidopropyl betaine, carbomer U20, isopropyl myristate, allantoin, quinoa saponin, sodium cocoyl methyl taurate, sodium laureth sulfate, beta-sitosterol, wheat germ extract, modified cod bone collagen peptide, walnut protein, linseed oil, essence, plant extract, 1, 3-propylene glycol, vitamin B5, an anti-dandruff compound and water. The anti-hair loss shampoo disclosed by the invention is safe, has no side effect, has good moisturizing, anti-hair loss and itching relieving effects, and has good silky and smooth feeling.

Description

Anti-hair loss shampoo and preparation method thereof

Technical Field

The invention belongs to the technical field of daily chemicals, and particularly relates to a hair loss preventing shampoo and a preparation method thereof.

Background

As one of indispensable bath products, people pay attention to the decontamination and cleaning capability of the shampoo and pay attention to the conditioning and nursing efficacy of the shampoo. The shampoo has various types, and has anti-dandruff shampoo, hair care shampoo, moisturizing shampoo, softening shampoo and the like according to the efficacy. With the acceleration of life and work rhythm, people have more and more pressure, and the problem of alopecia becomes a trouble for many people. The shampoo for preventing alopecia can care and nourish scalp hair quality, prevent alopecia and inhibit alopecia aggravation. The Chinese invention patent CN107823071A discloses an anti-hair loss shampoo, which consists of vetiver extract, geranium extract, Atlantic cedar extract, wild chrysanthemum extract, cacumen biotae soaking solution, sodium lauryl alcohol ether sulfate, disodium cocoyl amphodiacetate, biogan, lanolin, butanediol and plant antioxidant. Has the advantages of anti-alopecia and antipruritic effects, skin friendliness, strong permeability, and good hair and scalp cleaning effect.

Disclosure of Invention

In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a shampoo for preventing hair loss and a preparation method thereof.

The anti-hair loss shampoo comprises the following raw materials: chitosan quaternary ammonium salt, laurylamidopropyl betaine, carbomer U20, isopropyl myristate, allantoin, quinoa saponin, sodium cocoyl methyl taurate, sodium laureth sulfate, beta-sitosterol, wheat germ extract, modified cod bone collagen peptide, walnut protein, linseed oil, essence, plant extract, 1, 3-propylene glycol, vitamin B5 and water.

Preferably, the anti-hair loss shampoo is prepared from the following raw materials in parts by weight: 0.5-2 parts by weight of chitosan quaternary ammonium salt, 10-20 parts by weight of laurylamidopropyl betaine, 0.2-2 parts by weight of carbomer U20, 1-5 parts by weight of isopropyl myristate, 0.1-1.5 parts by weight of allantoin, 0.05-0.5 part by weight of quinoa saponin, 1-10 parts by weight of sodium cocoyl methyl taurate, 3-8 parts by weight of sodium laureth sulfate, 0.1-0.5 part by weight of beta-sitosterol, 1-3 parts by weight of wheat germ extract, 0.5-5 parts by weight of modified cod bone collagen peptide, 1-3 parts by weight of walnut protein, 0.3-5 parts by weight of linseed oil, 0.5-1 part by weight of essence, 5-15 parts by weight of plant extract, 1-3 parts by weight of 1, 3-propylene glycol, 0.1-0.5 part by weight of vitamin B5, 2-5 parts by weight of anti-dandruff compound, 80-100 parts by weight of water.

The anti-dandruff compound is prepared by the following method:

adding 5-8 parts by weight of new conazole into 15-20 parts by weight of glycerol at 70 ℃ and stirring for 10-15min at the stirring speed of 200-300rpm to obtain new conazole dispersion liquid; adding 5-10 parts by weight of hydrolyzed zein, 3-5 parts by weight of burdock root extract and the neoconazole dispersion liquid into 60-80 parts by weight of water, and stirring for 40-60min at the stirring speed of 500-600 rpm; adding 3-5 parts by weight of lemon oil, and continuously stirring for 5-8 min; homogenizing in a high-pressure homogenizer for 3-5min under 30-50 MPa.

The wheat germ extract contains unsaturated fatty acid, vitamin substances and various mineral substances, can provide rich nutrient substances for hair growth, can effectively keep scalp elasticity, and has the effects of resisting bacteria, repairing and preventing hair loss on hair and scalp; the linseed oil is rich in unsaturated fatty acid, has the effects of moisturizing and resisting allergy, can effectively protect pigment in hair follicles, enables the scalp to be black, bright and straight, and has the effects of protecting bioactivity and enhancing the adhesion of the linseed oil on the hairs. The walnut protein is rich in natural amino acids, effectively keeps the moisturizing effect of hair and scalp, simultaneously effectively activates hair follicle cells and effectively promotes hair growth. The beta-sitosterol has strong permeability, can keep the moisture of the skin and promote the growth of the skin, and has good anti-allergic and anti-inflammatory properties; quinoa saponin is a natural surfactant, has emulsifying, dispersing, washing, foam increasing, solubilizing abilities, can enhance scalp immunity, has good effects of resisting skin pathogenic fungi, diminishing inflammation and preventing corrosion, and has effects of washing hair, removing dandruff, relieving itching, keeping moisture and relieving; vitamin B5 can effectively penetrate scalp, improve hair luster, and reduce hair injury. The cod bone collagen peptide provides abundant nutrient components for scalp and hair growth, has good repairing and moisturizing functions on hair and scalp, effectively promotes hair growth, and improves hair toughness.

The plant extract is prepared from potentilla chinensis extract, polygonum divaricatum extract and mulberry bud tea extract according to the mass ratio of (2-3): (5-8): (3-5).

Potentilla chinensis Ser, a scientific name, and perennial herbs, and the Potentilla chinensis extract contains tormentic acid and oleanolic acid, and has the effects of promoting hair growth and inhibiting 5 alpha-reductase.

Polygonum divaricatum L, known as Polygonum divariatum L, is a plant of the genus Polygonum of the family Polygonaceae. The herba Polygoni Avicularis extractive solution contains hyperoside, quercitrin, and epigallocatechin, and can relieve scalp oil production.

The mulberry bud tea is prepared from tender buds of folium Mori (Moraceae, Morus) of Morus genus and folium et cacumen arbor. The folium Mori bud tea contains flavone extract, coumarin, amino acids, alkaloids, and aromatic oil; the mulberry tea is treated by combining the ionic liquid with microwave and enzymolysis, the content of effective substances is obviously improved, the ionic liquid is used as a solvent and a catalyst to be combined with organic groups on the surface of the mulberry tea through electrostatic and hydrogen bond effects to further dissolve cellulose, the microwave treatment is further carried out to cause the rupture of a cell membrane structure, and meanwhile, pectinase and maltase are combined to further carry out enzymolysis on substances in mulberry tea cells, so that the permeation of the solvent to the mulberry tea and the dissolution of the effective ingredients are accelerated. The mulberry bud tea extract contains xanthone compounds, soyabean essence, tea polyphenol, amino acids, alkaloids and aromatic oil, wherein the tea polyphenol not only can clean grease to prevent hair loss caused by hair follicle blockage, but also can sterilize, diminish inflammation and prevent various bacteria from breeding, and has good cleaning, antibacterial and repairing effects on scalp and hair.

The potentilla chinensis extract, the polygonum divaricatum extract and the mulberry bud tea extract have a synergistic effect according to a certain proportion, have the effects of improving scalp environment, preventing hair follicle blockage, regulating metabolism, simultaneously conveying energy and nutrient substances to scalp and hair and remarkably improving alopecia.

The preparation method of the potentilla extract comprises the following steps: cleaning Potentilla chinensis and drying; performing steam explosion on the dried potentilla chinensis under the steam pressure of 0.8-1.1Mpa for 0.01-0.03s and the pressure maintaining time of 15-20s to obtain the potentilla chinensis steam explosion material; pulverizing Potentilla chinensis steam explosion material, and sieving with 20-40 mesh sieve to obtain Potentilla chinensis powder; adding 500 parts by weight of potentilla chinensis powder, 5-10 parts by weight of peptone, 20-30 parts by weight of corn flour and 750 parts by weight of water in 200 parts by weight of-; 1g of bath ratio: (15-25) adding the fermented potentilla chinensis powder into water, uniformly mixing, performing ultrasonic treatment for 1-2h, wherein the ultrasonic power is 800-; adding the precipitate A and cellulase into water, performing enzymolysis for 5-8h at 35-50 ℃, and centrifuging to obtain a supernatant B, wherein the mass ratio of the precipitate A to the cellulase to the water is (10-20): (0.01-0.06): (30-50); mixing the supernatant A and B, concentrating under reduced pressure at 60-70 deg.C under vacuum degree of 0.06-0.1MPa to density of 1.00-1.10g/mL (25 deg.C), and making into Potentilla chinensis extractive solution.

The preparation method of the polygonum divaricatum extract comprises the following steps: cleaning and drying the leaves and roots of the divaricate knotweed; performing steam explosion on the dried polygonum divaricatum under the steam pressure of 0.8-1.1Mpa for 0.01-0.03s and the pressure maintaining time of 15-20s to obtain polygonum divaricatum steam explosion material; pulverizing the branch Polygonum divaricatum steam-exploded material, and sieving with 20-40 mesh sieve to obtain branch Polygonum divaricatum powder; adding 200 portions of minus knotweed powder, 5 to 10 portions of peptone, 20 to 30 portions of corn flour and 750 portions of water by weight into a triangular flask, uniformly stirring to obtain a mixture II, sterilizing the mixture II at 115 ℃ for 20min, adding strains accounting for 3 to 5 weight percent of the mixture II, fermenting for 8 to 12 days at 30 to 37 ℃, sterilizing at 115 ℃ for 15 to 20min after the fermentation is finished, drying and crushing the fermented substances after the sterilization to obtain the fermented minus knotweed powder; 1g of bath ratio: (15-25) adding the fermented polygonum divaricatum powder into water, uniformly mixing, performing ultrasonic treatment for 1-2h, wherein the ultrasonic power is 800-; adding the precipitate C and cellulase into water, performing enzymolysis for 5-8h at 35-50 ℃, and centrifuging to obtain a supernatant E, wherein the mass ratio of the precipitate C to the cellulase to the water is (10-20): (0.01-0.06): (30-50); mixing the supernatant C and E, concentrating under reduced pressure at 60-70 deg.C under vacuum degree of 0.06-0.1MPa to density of 1.00-1.10g/mL (25 deg.C) to obtain herba Polygoni Avicularis extractive solution.

The strain is one or a mixture of lactobacillus plantarum and bifidobacterium adolescentis; preferably, the strains are lactobacillus plantarum and bifidobacterium adolescentis according to the mass ratio of (1-5): (1-3) mixing.

The preparation method of the mulberry bud tea extracting solution comprises the following steps: pulverizing folium Mori, and sieving with 60-80 mesh sieve to obtain folium Mori tea powder; adding mulberry bud tea powder into the ionic liquid solution, uniformly mixing, performing microwave treatment for 10-20min, wherein the microwave power is 600W, filtering, removing filter residues, standing and layering the filtrate, and collecting an ionic liquid layer; extracting the ionic liquid layer for 2-3 times by adopting equal volume of ethyl acetate, combining ethyl acetate phases, sequentially washing by adopting water and saturated sodium chloride aqueous solution, collecting an organic layer, drying the organic layer by using anhydrous sodium sulfate, filtering, concentrating the filtrate under reduced pressure at the temperature of 60-70 ℃ under the vacuum degree of 0.06-0.1MPa, and concentrating to the density of 1.00-1.10g/mL (25 ℃) to obtain a mulberry bud tea extract, wherein the material-liquid ratio of the mulberry bud tea powder to the ionic liquid solution is 1 (8-15); the ionic liquid solution is prepared from ionic liquid, absolute ethyl alcohol and water according to the mass ratio (0.1-0.2): (70-80): (20-30) mixing to obtain the product.

The ionic liquid is one or a mixture of 1-butyl-3-methylimidazole acetate and 1-butyl-3-methylimidazole mesylate; preferably, the ionic liquid is prepared from 1-butyl-3-methylimidazole acetate and 1-butyl-3-methylimidazole methanesulfonate according to the mass ratio of (1-3): (1-3) mixing.

Modifying the cod bone collagen peptide by taking trehalose and hyaluronic acid as raw materials to prepare modified cod bone collagen peptide; the anti-hair loss shampoo adopts transglutaminase as a cross-linking agent to cross-link epsilon-polylysine and cod bone collagen peptide and graft the epsilon-polylysine and the cod bone collagen peptide on trehalose and hyaluronic acid, so that the moisturizing performance of the anti-hair loss shampoo is obviously improved, and the stability of a plant extracting solution is improved. Hyaluronic acid and trehalose have good water solubility, and epsilon-polylysine and cod bone collagen peptide are grafted to form a three-dimensional network structure under the action of transglutaminase, so that the moisturizing performance is improved.

The preparation method of the modified cod bone collagen peptide comprises the following steps:

(1) cleaning the cod bone, drying at 50-60 ℃ for 10-12h, crushing, and sieving with a 100-mesh and 200-mesh sieve to obtain cod bone powder; adding the cod bone powder and the enzyme preparation into water, uniformly stirring, carrying out enzymolysis for 2-5h at 40-50 ℃, then heating to 100 ℃, extinguishing fire for 5-8min, centrifuging, taking supernatant, and freeze-drying to obtain the cod bone collagen peptide, wherein the mass ratio of the cod bone powder, the enzyme preparation and the water is (9-15): (1-2): (100- > 200); the enzyme preparation is prepared from trypsin and alkaline protease according to the mass ratio of 1: (1-2) mixing;

(2) adding monomer, epsilon-polylysine and cod bone collagen peptide into 0.2mol/L NaH₂PO₄-Na₂HPO₄Mixing the buffer solution uniformly to obtain a mixed solution, wherein the monomer, the epsilon-polylysine, the cod bone collagen peptide and the NaH are mixed uniformly₂ PO₄-Na₂ HPO₄The mass ratio of the buffer solution is 1 (1-3) to (50-120); adding a transglutaminase solution, stirring at the temperature of 30-40 ℃ and the rotating speed of 200-400 rpm for 5-8h, heating to 100 ℃ after the reaction is finished, extinguishing the fire for 5-8min, performing suction filtration and freeze drying to obtain the modified cod bone collagen peptide, wherein the mass ratio of the transglutaminase solution to the mixed solution is (1-2): (5-6); the transglutaminase solution is prepared by adding 0.1-0.5g transglutaminase into 10mL of 0.2-0.5mol/LNaH₂PO₄-Na₂HPO₄The buffer solution is mixed evenly.

The monomer is one or a mixture of trehalose and hyaluronic acid; preferably, the monomer is prepared from trehalose and hyaluronic acid according to a mass ratio of (1-3): (1-3) mixing.

The preparation method of the anti-hair loss shampoo comprises the following steps:

s1, adding carbomer U20 and sodium cocoyl methyl taurate into water, uniformly mixing, heating to 80-85 ℃, stirring at the rotating speed of 100-300 revolutions per minute for 20-30 minutes, sequentially adding sodium laureth sulfate, chitosan quaternary ammonium salt, lauramidopropyl betaine and 1, 3-propylene glycol, keeping the temperature at 80-85 ℃, and stirring at the rotating speed of 100-300 revolutions per minute for 30-50 minutes;

s2, cooling to 50-60 ℃, then sequentially adding plant extract, quinoa saponin, wheat germ extract, modified fishbone collagen peptide, walnut protein, vitamin B5 and anti-dandruff compound, uniformly mixing, and stirring at 50-60 ℃ at the rotating speed of 100 revolutions per minute for 30-40 minutes;

s3, cooling to 30-45 ℃, sequentially adding isopropyl myristate, allantoin, beta-sitosterol, linseed oil and essence, uniformly mixing, and stirring at 30-45 ℃ at the rotating speed of 300 revolutions per minute of 100-40 minutes;

s4 cooling to 28-32 deg.C, standing for 12-24 hr, and canning to obtain shampoo for preventing alopecia.

The invention has the beneficial effects that: the anti-hair loss shampoo disclosed by the invention is safe, has no side effect, has good moisturizing, anti-hair loss and itching relieving effects, and has good silky and smooth feeling. The adopted plant extracting solution is composed of potentilla chinensis extracting solution, polygonum divaricatum extracting solution and mulberry bud tea extracting solution according to a specific ratio, and the synergistic effect can improve scalp environment, prevent hair follicle blockage, regulate metabolism, simultaneously convey energy and nutrient substances for scalp and hair and obviously improve alopecia effect.

Detailed Description

The above summary of the present invention is described in further detail below with reference to specific embodiments, but it should not be understood that the scope of the above subject matter of the present invention is limited to the following examples.

Chitosan quaternary ammonium salt, cat number: s26618, Shanghai-derived leaf Biotechnology, Inc.

Lauramidopropyl betaine, CAS: 4292-10-8, purity: 99%, Chongqing Rui ya Biotech Co.

Carbomer U20, lubrian specialty chemicals manufacturing (shanghai) ltd.

Isopropyl myristate, CAS: 110-27-0, purity: 98%, Shanghai Michelin Biochemical technology, Inc.

Quinoa saponin is prepared according to patent 201610529182.0, example 3.

Sodium cocoyl methyl taurate, CAS: 12765-39-8, Shanghai Kao Jing industries, Ltd.

Sodium laureth sulfate, CAS: 15826-16-1, purity: 99%, Wuhan Carnous technologies, Inc.

Beta-sitosterol, CAS: 83-46-5, purity: 99%, Hubei Huishaipu medicine science and technology Limited.

Wheat germ extract, Shanxi Senyuan Biotech limited.

Walnut protein, purity: 99% by sienna eastern peak biotechnology limited.

Linseed oil, CAS: 8001-26-1, purity: 99%, Shanghai Michelin Biochemical technology, Inc.

Vitamin B5, CAS: 137-08-6, shanghai ezh biotechnology limited.

Cellulase, CAS number: 9042-54-8, enzyme activity 20000U/g, Shandong Su Kehan bioengineering GmbH.

The essence is orange essence, powder, food grade, Shenzhen topology biotechnology limited.

The preparation method of the potentilla extract comprises the following steps: cleaning Potentilla chinensis and drying; performing steam explosion on the dried potentilla chinensis, wherein the steam pressure is 0.8Mpa, the steam explosion time is 0.01s, and the pressure maintaining time is 20s, so as to obtain the potentilla chinensis steam explosion material; pulverizing Potentilla chinensis steam explosion material, and sieving with 40 mesh sieve to obtain Potentilla chinensis powder; adding 200 parts by weight of potentilla chinensis powder, 5 parts by weight of peptone, 20 parts by weight of corn flour and 350 parts by weight of water into a triangular flask, uniformly stirring to obtain a mixture I, sterilizing the obtained mixture I at 115 ℃ for 20min, adding a strain accounting for 3 wt% of the weight of the mixture I, fermenting at 37 ℃ for 10 days, sterilizing at 115 ℃ for 20min after fermentation is finished, drying and crushing to obtain fermented potentilla chinensis powder; 1g of bath ratio: adding 15mL of the fermented potentilla powder into water, uniformly mixing, performing ultrasonic treatment for 2h, wherein the ultrasonic power is 800W, the ultrasonic frequency is 20kHz, and centrifuging to obtain a supernatant A and a precipitate A; adding the precipitate A and cellulase into water, performing enzymolysis for 6h at 45 ℃, and centrifuging to obtain a supernatant B, wherein the mass ratio of the precipitate A to the cellulase to the water is 10: 0.05: 45, a first step of; mixing the supernatant A and B, concentrating under reduced pressure at 60 deg.C under 0.06MPa, and concentrating to density of 1.10g/mL (25 deg.C) to obtain herba Potentillae chinensis extractive solution. The strain is lactobacillus plantarum and bifidobacterium adolescentis according to the mass ratio of 2: 1 and mixing.

The preparation method of the polygonum divaricatum extract is the same as that of the potentilla chinensis extract, and the difference is only that the potentilla chinensis is replaced by the polygonum divaricatum.

The preparation method of the mulberry bud tea extract comprises the following steps: crushing the mulberry bud tea, and sieving the crushed mulberry bud tea with a 60-mesh sieve to obtain mulberry leaf tea powder; adding mulberry bud tea powder into an ionic liquid solution, uniformly mixing, performing microwave treatment for 20min, wherein the microwave power is 600W, the material-liquid ratio of the mulberry bud tea powder to the ionic liquid solution is 1g:10mL, filtering, removing filter residues, standing and layering the filtrate, and collecting an ionic liquid layer; extracting an ionic liquid layer for 3 times by adopting equal volume of ethyl acetate, combining ethyl acetate phases, sequentially washing by adopting water and a saturated sodium chloride aqueous solution, collecting an organic layer, drying the organic layer by using anhydrous sodium sulfate, filtering, concentrating filtrate under reduced pressure at the temperature of 60 ℃ under the vacuum degree of 0.06MPa until the concentration is 1.10g/mL (25 ℃) to obtain a mulberry bud tea extracting solution, wherein the ionic liquid solution is prepared by mixing ionic liquid, anhydrous ethanol and water according to the mass ratio of 0.1: 80: 20 are mixed to obtain; the ionic liquid is prepared from 1-butyl-3-methylimidazole acetate and 1-butyl-3-methylimidazole mesylate according to a mass ratio of 1: 1.

Potentilla chinensis is Potentilla chinensis, a company Limited in the pharmaceutical industry of Yihongtang, Bozhou city.

The Bianfen Polygonum is collected from Xingjiang Lanzhen in Nengyun Chifeng city.

Mulberry bud tea, xiajin holy tree source agriculture ltd.

1-butyl-3-methylimidazole acetate, CAS: 284049-75-8, purity: 98%, Shanghai Michelin Biochemical technology, Inc.

1-butyl-3-methylimidazole mesylate, CAS: 342789-81-5, purity: 99%, Shanghai Michelin Biochemical technology, Inc.

Lactobacillus plantarum, accession number: CCTCC AB 2010330, latin literature name: lactobacillus plantarum, China center for type culture Collection.

Bifidobacterium adolescentis, accession number: cic 6175, latin literature name: bifidobacterium adolensis, China center for the Collection of Industrial microorganisms.

Trypsin, enzyme activity: 100000U/mg, food grade, dragon and chemical company, province of Henan province.

The alkaline protease with the enzyme activity of 20 ten thousand U/g is purchased from Ningxia Xixia industry group Limited.

Epsilon-polylysine, CAS: 28211-04-3, relative molecular weight of 3000, Nanjing Doulei Biotechnology, Inc.

Transglutaminase, enzyme activity 10 uw/g, Siranavira Biotech Co., Ltd.

The cod bone is bone of cod (Latin literature named gadus macrocephalus) belonging to family Gadidae.

Trehalose, CAS: 99-20-7; hyaluronic acid, CAS: 9004-61-9; neoconazole, CAS: 67914-69-6; burdock root extract, CAS: 84012-13-5; hydrolyzed zein, CAS: 73049-73-7; lemon oil, CAS: 8008-56-8.

Example 1

The anti-hair loss shampoo is composed of the following raw materials in parts by weight: 0.5 part by weight of chitosan quaternary ammonium salt, 15 parts by weight of laurylamidopropyl betaine, 0.2 part by weight of carbomer U20, 2 parts by weight of isopropyl myristate, 0.2 part by weight of allantoin, 0.1 part by weight of quinoa saponin, 8 parts by weight of sodium cocoyl methyltaurate, 5 parts by weight of sodium laureth sulfate, 0.3 part by weight of beta-sitosterol, 3 parts by weight of wheat germ extract, 2 parts by weight of cod bone collagen peptide, 1 part by weight of walnut protein, 0.5 part by weight of linseed oil, 0.5 part by weight of essence, 8 parts by weight of plant extract, 2 parts by weight of 1, 3-propylene glycol, 0.2 part by weight of vitamin B5 and 100 parts by weight of water.

The plant extract is prepared from potentilla chinensis extract, polygonum divaricatum extract and mulberry bud tea extract according to the mass ratio of 2: 5: 3, and (3).

s1 adding carbomer U20 and sodium cocoyl methyl taurate into water, uniformly mixing, heating to 80 ℃, stirring for 30 minutes at the rotating speed of 300 revolutions per minute, sequentially adding sodium laureth sulfate, chitosan quaternary ammonium salt, lauramidopropyl betaine and 1, 3-propylene glycol, keeping the temperature at 80 ℃, and stirring for 50 minutes at the rotating speed of 300 revolutions per minute;

s2, cooling to 60 ℃, sequentially adding plant extract, quinoa saponin, wheat germ extract, fish bone collagen peptide, walnut protein and vitamin B5, uniformly mixing, and stirring at 60 ℃ at the rotating speed of 300 r/min for 40 min;

s3, cooling to 45 ℃, sequentially adding isopropyl myristate, allantoin, beta-sitosterol, linseed oil and essence, uniformly mixing, and stirring at 45 ℃ at the rotating speed of 300 r/min for 40 minutes;

s4, cooling to 32 ℃, standing for 24 hours, and canning to obtain the anti-hair loss shampoo.

Comparative example 1

Essentially the same as example 1, except that: no plant extract is added.

Example 2

Essentially the same as example 1, except that: the plant extract is herba Polygoni Avicularis extract.

Example 3

Essentially the same as example 1, except that: the plant extract is prepared from potentilla chinensis extract and polygonum divaricatum extract according to the mass ratio of 1: 1.

Example 4

The anti-hair loss shampoo is composed of the following raw materials in parts by weight: 0.5 part by weight of chitosan quaternary ammonium salt, 15 parts by weight of laurylamidopropyl betaine, 0.2 part by weight of carbomer U20, 2 parts by weight of isopropyl myristate, 0.2 part by weight of allantoin, 0.1 part by weight of quinoa saponin, 8 parts by weight of sodium cocoyl methyltaurate, 5 parts by weight of sodium laureth sulfate, 0.3 part by weight of beta-sitosterol, 3 parts by weight of wheat germ extract, 2 parts by weight of modified cod bone collagen peptide, 1 part by weight of walnut protein, 0.5 part by weight of linseed oil, 0.5 part by weight of essence, 8 parts by weight of plant extract, 2 parts by weight of 1, 3-propylene glycol, 0.2 part by weight of vitamin B5 and 100 parts by weight of water.

(1) cleaning cod bone, drying at 60 deg.C for 12 hr, pulverizing, and sieving with 100 mesh sieve to obtain cod bone powder; adding the cod bone powder and the enzyme preparation into water, uniformly stirring, carrying out enzymolysis at 50 ℃ for 5h, heating to 100 ℃, inactivating for 5min, centrifuging, taking supernatant, and freeze-drying to obtain cod bone collagen peptide, wherein the mass ratio of the cod bone powder, the enzyme preparation and the water is 9: 1: 100, respectively; the enzyme preparation is prepared from trypsin and alkaline protease according to the mass ratio of 1: 1, mixing;

(2) adding monomer, epsilon-polylysine and cod bone collagen peptide into 0.2mol/L NaH₂PO₄-Na₂ HPO₄Mixing the buffer solution uniformly to obtain a mixed solution, wherein the monomer, the epsilon-polylysine, the cod bone collagen peptide and the NaH are mixed uniformly₂PO₄-Na₂HPO₄The mass ratio of the buffer solution is 1: 3: 3: 120 of a solvent; and adding a transglutaminase solution, wherein the mass ratio of the transglutaminase solution to the mixed solution is 2: 5; stirring at 40 ℃ and a rotating speed of 400 r/min for 6h, heating to 100 ℃ after the reaction is finished, inactivating for 5min, performing suction filtration, and freeze-drying to obtain modified cod bone collagen peptide; the transglutaminase solution is prepared by adding 0.1g transglutaminase into 10ml of 0.2mol/L NaH₂ PO₄-Na₂ HPO₄The buffer solution is mixed evenly. The monomer is prepared from trehalose and hyaluronic acid according to a mass ratio of 2: 1 and mixing.

s2, cooling to 60 ℃, sequentially adding plant extract, quinoa saponin, wheat germ extract, modified fish bone collagen peptide, walnut protein and vitamin B5, uniformly mixing, and stirring at 60 ℃ at the rotating speed of 300 r/min for 40 min;

Example 5

Essentially the same as example 4, except that: the preparation method of the modified cod bone collagen peptide comprises the following steps:

(2) adding monomer, epsilon-polylysine and cod bone collagen peptide into 0.2mol/L NaH₂PO₄-Na₂ HPO₄Mixing the buffer solution uniformly to obtain a mixed solution, wherein the monomer, the epsilon-polylysine, the cod bone collagen peptide and the NaH are mixed uniformly₂PO₄-Na₂HPO₄The mass ratio of the buffer solution is 1: 3: 3: 120 of a solvent; and adding a transglutaminase solution, wherein the mass ratio of the transglutaminase solution to the mixed solution is 2: 5; stirring at 40 ℃ and a rotating speed of 400 r/min for 6h, heating to 100 ℃ after the reaction is finished, inactivating for 5min, performing suction filtration, and freeze-drying to obtain modified cod bone collagen peptide; the transglutaminase solution is prepared by adding 0.1g transglutaminase into 10ml of 0.2mol/L NaH₂ PO₄-Na₂ HPO₄The buffer solution is mixed evenly. The monomer is trehalose.

Example 6

(2) adding monomer, epsilon-polylysine and cod bone collagen peptide into 0.2mol/L NaH₂PO₄-Na₂ HPO₄Mixing the buffer solution uniformly to obtain a mixed solution, wherein the monomer, the epsilon-polylysine, the cod bone collagen peptide and the NaH are mixed uniformly₂PO₄-Na₂HPO₄The mass ratio of the buffer solution is 1: 3: 3: 120 of a solvent; and adding a transglutaminase solution, wherein the mass ratio of the transglutaminase solution to the mixed solution is 2: 5; stirring at 40 ℃ and a rotating speed of 400 r/min for 6h, heating to 100 ℃ after the reaction is finished, inactivating for 5min, performing suction filtration, and freeze-drying to obtain modified cod bone collagen peptide; the transglutaminase solution is prepared by adding 0.1g transglutaminase into 10ml of 0.2mol/L NaH₂ PO₄-Na₂ HPO₄The buffer solution is mixed evenly. The monomer is hyaluronic acid.

Example 7

The anti-hair loss shampoo is composed of the following raw materials in parts by weight: 0.5 part by weight of chitosan quaternary ammonium salt, 15 parts by weight of laurylamidopropyl betaine, 0.2 part by weight of carbomer U20, 2 parts by weight of isopropyl myristate, 0.2 part by weight of allantoin, 0.1 part by weight of quinoa saponin, 8 parts by weight of sodium cocoyl methyltaurate, 5 parts by weight of sodium laureth sulfate, 0.3 part by weight of beta-sitosterol, 3 parts by weight of wheat germ extract, 2 parts by weight of modified cod bone collagen peptide, 1 part by weight of walnut protein, 0.5 part by weight of linseed oil, 0.5 part by weight of essence, 8 parts by weight of plant extract, 2 parts by weight of 1, 3-propylene glycol, 0.2 part by weight of vitamin B5, 3.5 parts by weight of anti-dandruff compound and 100 parts by weight of water.

The anti-dandruff compound is prepared by the following method:

adding 7 parts by weight of neoconazole into 15 parts by weight of glycerol with the temperature of 70 ℃, and stirring for 10min at the stirring speed of 200rpm to obtain neoconazole dispersion liquid; adding 10 parts by weight of hydrolyzed zein, 5 parts by weight of burdock root extract and the neoconazole dispersion liquid into 65 parts by weight of water, and stirring for 50min at the stirring speed of 500 rpm; adding 3 parts by weight of lemon oil, and continuously stirring for 5 min; homogenizing in a high pressure homogenizer for 5min under 30 MPa.

s2, cooling to 60 ℃, sequentially adding plant extract, quinoa saponin, wheat germ extract, modified fish bone collagen peptide, walnut protein, vitamin B5 and anti-dandruff compound, uniformly mixing, and stirring at 60 ℃ at the rotating speed of 300 r/min for 40 min;

Example 8

The anti-dandruff compound is prepared by the following method:

adding 7 parts by weight of neoconazole into 15 parts by weight of glycerol with the temperature of 70 ℃, and stirring for 10min at the stirring speed of 200rpm to obtain neoconazole dispersion liquid; adding the neoconazole dispersion into 80 parts by weight of water, and stirring for 50min at the stirring speed of 500 rpm; adding 3 parts by weight of lemon oil, and continuously stirring for 5 min; homogenizing in a high pressure homogenizer for 5min under 30 MPa.

Test example 1

And (3) testing the anti-hair loss effect: the test subjects were 210 male alopecia patients, divided into 7 groups of 30. The anti-hair loss shampoos prepared in examples 1-6 of the present invention and comparative example 1 were used in an amount of 30mL each time every 3 days for 7 groups of subjects, and were evaluated for the anti-hair loss effect after 16 weeks without using other hair washing and hair care products except the Chinese herbal anti-hair loss shampoo of the present invention, and without performing hair dyeing, hair waving and other hair cosmetic actions during the test period.

The evaluation standard of the curative effect is as follows:

and (3) curing: the hair is not dropped, or the hair loss is less than 25 in every day, the hair is soft, smooth in combing, black in hair color, free of bifurcation, withered and yellow, fresh and cool in scalp, clear in head and moist in hair;

the effect is shown: the hair loss is obviously reduced, or the hair loss is between 25 and 50 in each day, the hair is soft, smooth in combing, black in hair color, basically free of bifurcation, withered and yellow, refreshing in skin, clear in head and moist in hair;

the method has the following advantages: the hair loss phenomenon still exists, but the hair loss is between 50 and 75 in each day, the hair quality is softer, the hair is easier to comb, the hair color is blackened, basically has no bifurcation, withered and yellow, the skin is more refreshing, the head is clearer, and the hair is more moist.

And (4) invalidation: the alopecia phenomenon is not changed, or the daily alopecia is more than 75, the hair is hard and crisp, difficult to comb, the hair quality is dry, and the hair color is scorched.

TABLE 1 Hair loss prevention Effect test results of Hair loss prevention shampoo

Test example 2

And (3) testing the carding performance: this was done using a hair tester brand DIASTRON, model MTT 175. The tests included dry combing, wet combing tests, which were: cleaning 0.3g hair bundle, moistening with 2g 10% sodium lauryl sulfate water solution for 1min, washing with clear water, combing once with comb to remove excessive water, and measuring wet hair combing power W with hair tester₁The above steps were repeated, 3 times for each sample and the average was taken. Drying the hair sample in air for 24 hr after wet combing test, measuring the dry combing power, repeating the above steps, measuring each sample for 3 times, and taking average value W₂. Separately, 0.3g of hair tresses were washed, moistened with 2g of the anti-hair loss shampoos prepared in examples 1-6 and comparative example 1 for 1min, washed clean with clear water, combed once with a comb to remove excess moisture, and the wet hair combing power W was measured with a hair tester₁', repeat the above steps, each sample is measured 3 times, and take the average value; after the wet combing test was completed, the hair sample was dried in the air for 24 hours, and the dry combing work W was measured₂', repeat the above steps, measure 3 times per sample, and take the average. The carding work reduction value is calculated. Wet hair combing work reduction value (W ═₁-W₁')/W₁X 100%. Dry carding work reduction value (W)₂-W₂′)/W₂×100％。

TABLE 2 Hair loss prevention shampoo combing work test results

As shown in Table 2, the anti-hair loss shampoo containing the modified codfish ossein peptide can moisten hair and improve the problem that the hair is dry and difficult to comb. Modifying the cod bone collagen peptide by taking trehalose and hyaluronic acid as raw materials to prepare modified cod bone collagen peptide; the anti-hair loss shampoo adopts transglutaminase as a cross-linking agent to cross-link epsilon-polylysine and cod bone collagen peptide and graft the epsilon-polylysine and the cod bone collagen peptide on trehalose and hyaluronic acid, so that the moisturizing performance of the anti-hair loss shampoo is obviously improved, and the stability of a plant extracting solution is improved. Hyaluronic acid and trehalose have good water solubility, and epsilon-polylysine and cod bone collagen peptide are grafted to form a three-dimensional network structure under the action of transglutaminase, so that the moisturizing performance is improved.

Test example 3

And (3) testing the chip removal performance: a 10-point scoring method was used. Wherein, 0 grade is without scurf; the l-2 level is small powdery grey rough scale; 3-4 grades are small to medium sized chips; 5-6 grades are large, thin chips loosely associated with the scalp; large adhesive chips on a 7-8 scale; grades 9-10 are thick scales from yellow to closely attached to the scalp. The test subjects were 60 female subjects with dandruff grade 7-8, divided into two groups of 30 subjects, who were shampooed with the hair loss preventing shampoo of examples 7-8, respectively, at a frequency of once every 3 days. During the test period, other washing and hair care products except the anti-hair loss shampoo of the invention are not used, and hair dyeing, hair perming and the like are not carried out. After five weeks, the scalp and the dandruff attached to the hair within 4cm from the root were rated according to a 10-point rating method.

TABLE 3 anti-alopecia shampoo antidandruff Performance test results

	Grade of antidandruff
		Example 7	Level 0
Example 8	Stage 2

As can be seen from Table 3, the shampoo for preventing alopecia of the invention has good anti-dandruff efficacy, wherein the anti-dandruff compound adopted can inhibit the biosynthesis of fungi maltol, kill candida, dermatitis blastomyces and the like, regulate skin grease, and effectively remove dandruff.

Claims

1. The anti-hair loss shampoo is characterized in that: the method comprises the following raw materials: chitosan quaternary ammonium salt, laurylamidopropyl betaine, carbomer U20, isopropyl myristate, allantoin, quinoa saponin, sodium cocoyl methyl taurate, sodium laureth sulfate, beta-sitosterol, wheat germ extract, modified cod bone collagen peptide, walnut protein, linseed oil, essence, plant extract, 1, 3-propylene glycol, vitamin B5, an anti-dandruff compound and water.

2. The anti-hair loss shampoo according to claim 1, wherein: the composition is characterized by comprising the following raw materials in parts by weight: 0.5-2 parts by weight of chitosan quaternary ammonium salt, 10-20 parts by weight of laurylamidopropyl betaine, 0.2-2 parts by weight of carbomer U20, 1-5 parts by weight of isopropyl myristate, 0.1-1.5 parts by weight of allantoin, 0.05-0.5 part by weight of quinoa saponin, 1-10 parts by weight of sodium cocoyl methyl taurate, 3-8 parts by weight of sodium laureth sulfate, 0.1-0.5 part by weight of beta-sitosterol, 1-3 parts by weight of wheat germ extract, 0.5-5 parts by weight of modified cod bone collagen peptide, 1-3 parts by weight of walnut protein, 0.3-5 parts by weight of linseed oil, 0.5-1 part by weight of essence, 5-15 parts by weight of plant extract, 1-3 parts by weight of 1, 3-propylene glycol, 0.1-0.5 part by weight of vitamin B5, 2-5 parts by weight of anti-dandruff compound, 80-100 parts by weight of water.

3. The anti-hair loss shampoo according to claim 1 or 2, characterized in that: the anti-dandruff compound is prepared by the following method:

4. The anti-hair loss shampoo according to claim 1 or 2, characterized in that: the plant extract is prepared from potentilla chinensis extract, polygonum divaricatum extract and mulberry bud tea extract according to the mass ratio of (2-3): (5-8): (3-5).

5. The anti-hair loss shampoo according to claim 1 or 2, characterized in that: the preparation method of the modified cod bone collagen peptide comprises the following steps:

(2) adding monomer, epsilon-polylysine and cod bone collagen peptide into 0.2mol/L NaH₂PO₄-Na₂HPO₄Mixing the buffer solution uniformly to obtain a mixed solution, wherein the monomer, the epsilon-polylysine, the cod bone collagen peptide and the NaH are mixed uniformly₂ PO₄-Na₂ HPO₄The mass ratio of the buffer solution is 1 (1-3) to (50-120); then is added toAnd (2) stirring the transglutaminase solution at the temperature of 30-40 ℃ and the rotating speed of 200-400 rpm for 5-8h, heating to 100 ℃ after the reaction is finished, extinguishing the fire for 5-8min, performing suction filtration and freeze drying to obtain the modified cod bone collagen peptide, wherein the mass ratio of the transglutaminase solution to the mixed solution is (1-2): (5-6).

6. The anti-hair loss shampoo according to claim 5, wherein: the monomer is one or a mixture of trehalose and hyaluronic acid.

7. The method for preparing the shampoo for preventing hair loss according to any one of claims 1 to 6, wherein the shampoo for preventing hair loss comprises: the method comprises the following steps: