CN116195533A - Method for inducing female Hippocampus japonicus to naturally flow eggs based on breeding competition principle - Google Patents
Method for inducing female Hippocampus japonicus to naturally flow eggs based on breeding competition principle Download PDFInfo
- Publication number
- CN116195533A CN116195533A CN202310213985.5A CN202310213985A CN116195533A CN 116195533 A CN116195533 A CN 116195533A CN 202310213985 A CN202310213985 A CN 202310213985A CN 116195533 A CN116195533 A CN 116195533A
- Authority
- CN
- China
- Prior art keywords
- hippocampus
- female
- target
- female hippocampus
- culture container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000013601 eggs Nutrition 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000001939 inductive effect Effects 0.000 title claims abstract description 9
- 238000009395 breeding Methods 0.000 title abstract description 14
- 230000001488 breeding effect Effects 0.000 title abstract description 14
- 241001478425 Hippocampus japonicus Species 0.000 title abstract description 8
- 210000001320 hippocampus Anatomy 0.000 claims abstract description 101
- 210000004681 ovum Anatomy 0.000 claims abstract description 27
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 22
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 22
- 210000001015 abdomen Anatomy 0.000 claims abstract description 13
- 210000002149 gonad Anatomy 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000000971 hippocampal effect Effects 0.000 claims description 4
- 230000035935 pregnancy Effects 0.000 claims description 4
- 238000011161 development Methods 0.000 claims description 3
- 238000006213 oxygenation reaction Methods 0.000 claims description 3
- 230000006698 induction Effects 0.000 abstract description 7
- 230000008901 benefit Effects 0.000 abstract description 5
- 230000033228 biological regulation Effects 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 4
- 241001559542 Hippocampus hippocampus Species 0.000 description 15
- 101000831205 Danio rerio Dynein axonemal assembly factor 11 Proteins 0.000 description 14
- 102100024282 Dynein axonemal assembly factor 11 Human genes 0.000 description 14
- 101000831210 Homo sapiens Dynein axonemal assembly factor 11 Proteins 0.000 description 14
- 241001247203 Syngnathidae Species 0.000 description 6
- 230000001850 reproductive effect Effects 0.000 description 5
- 229920000742 Cotton Polymers 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 241001559546 Hippocampus erectus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a method for inducing female Hippocampus japonicus to naturally flow eggs based on a breeding competition principle. The method comprises the following steps: (A) Placing the target female Hippocampus with inflated abdomen and plump gonad, with ovum to be collected in a culture container; (B) Taking the female hippocampus which has spawned and has not been full in gonad or has not been full for a plurality of days from the next spawning as a non-target female hippocampus, and placing the non-target female hippocampus into a culture container; (C) placing the non-pregnant male hippocampus into a culture container; and (D) observing the egg flowing condition of the culture container at regular time. The method has the advantages of short induction time, high induction rate, no damage to the target female Hippocampus, and the collected ovum has the advantages of complete structure, pure components, clear quantity and the like, and has important significance for researching the propagation mechanism, propagation performance, propagation regulation and control, artificial propagation technology and the like of the Hippocampus cinerea.
Description
Technical Field
The invention belongs to the field of sea horses, and particularly relates to a method for inducing female gray sea horses to naturally flow eggs based on a breeding competition principle.
Background
The ovum is an indispensable material for researching animal reproduction mechanism, reproduction performance, reproduction regulation and control, artificial reproduction technology and the like. The quality of an ovum is directly related to the development of an embryo and the survival and growth of larvae. In recent decades, the quality of ova is increasingly emphasized in the artificial breeding and cultivation process of teleosts. The gray sea horse (Hippocampus erectus) is a small-sized teleosts, and large-scale artificial culture is carried out in China for more than ten years, so that the problem of low breeding efficiency of parent sea horse fishes is continuously exposed in recent years. For this reason, many researchers have locked their eyes to the quality of the eggs of female hippocampus, such as whether there is a decline in the quality of the eggs, whether there is a decrease in the conversion rate of the eggs to larvae, the direction of the untransformed eggs, how to improve the quality of the eggs, and the like. These studies were conducted on the premise that eggs of the female hippocampus could be collected smoothly.
The sea horse differs from the conventional fish in that the reproductive mode is fertilization in vivo and development in vivo. Female Hippocampus spawns eggs into the infant sacs of male Hippocampus, fertilization is completed in the infant sacs and embryo development is carried out, the embryo is hatched into independent larvae by the male Hippocampus, and finally the larvae are discharged from the infant sacs. The special reproductive way of the hippocampus determines that it is not as easy to collect ova as in vitro fertilized fish. The current common method for collecting the female hippocampus ovum comprises the following steps: (1) The target female hippocampus is paired with the male hippocampus, and the ova are collected by dissecting the male hippocampal infant-raising sac after the mating is expected to be successful. The eggs collected by the method are doped with semen components on one hand, and on the other hand, a plurality of eggs are mutually extruded in a narrow space of the child-bearing sac to cause morphological changes and even cracks, so that information such as quantity, egg diameter, egg yolk position, fat droplet size and distribution and the like cannot be accurately displayed. Further, more precisely, the fertilized egg is collected not as an egg. In addition, this method has the disadvantage that the male hippocampus must be killed. (2) Dissecting the target female Hippocampus, taking the ovary and collecting ovum. This approach causes death of the targeted female hippocampus, rendering it unusable for subsequent consistent scientific research, such as continuous reproductive performance monitoring, etc. In addition, the timing is not easy to grasp, and mature ova are not easy to collect by the method. (3) Waiting for the target female Hippocampus to spontaneously drain the ovum directly into water, thereby collecting the ovum in water. Earlier work by the inventors has found that female hippocampus with full gonads sometimes has the phenomenon of draining ova directly into the water due to interference of certain factors, which is often referred to as "egg flowing". Although the ovum collected by the method is truly the ovum in the strict sense, the method has large randomness and low control degree, because the timing of the female Hippocampus for directly draining the ovum into water is difficult to control; some female hippocampus, which are full in gonad but not paired with the male hippocampus, do not develop eggs during one or two months, or even longer.
Therefore, the invention provides a method which can induce the natural spawn flowing of the target female Hippocampus, has controllable spawn flowing time and no damage to the target female Hippocampus, and has important significance for researching the propagation mechanism, propagation performance, propagation regulation and control, artificial propagation technology and the like of the Hippocampus.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for inducing female Hippocampus japonicus to naturally flow eggs based on a propagation competition principle, so as to overcome the defects that in the prior art, the female Hippocampus japonicus is required to be dissected for ovum collection, the ovum is impure or the timing of flowing eggs is difficult to control and the like.
The invention provides a method for inducing female Hippocampus japonicus to naturally flow eggs, which comprises the following steps:
(A) Placing the target female Hippocampus with inflated abdomen and plump gonad, with ovum to be collected in a culture container;
(B) Taking the female hippocampus which has spawned and has not been full in gonad or has not been full for a plurality of days from the next spawning as a non-target female hippocampus, and placing the non-target female hippocampus into the culture container in the step (A);
(C) Placing the male hippocampus which is not pregnant in a culture container where the target female hippocampus and the non-target female hippocampus are located;
(D) Observing the egg flowing condition of the culture container at regular time.
Preferably, the target female sea mantissa in step (a) is 1 tail.
Preferably, in step (a) the target female hippocampus is marked to distinguish between non-target female hippocampus, e.g. the target female hippocampus has a thin cotton thread tied to the neck of the target female hippocampus to distinguish between non-target female hippocampus.
Preferably, the cultivation conditions of the cultivation container in the step (a) are as follows: the cultivation container has a volume of 400-500L, contains 300-400L of cultivation water, a water depth of 0.8-0.9 m, a water temperature of 24-26 ℃, and the cultivation water is continuously inflated for oxygenation.
Preferably, the cultivation container in the step (a) is a cultivation bucket.
Preferably, the non-target female hippocampus in step (B) is the same as or similar to the target female hippocampus. The same or similar height is to exacerbate the competitive strength.
Preferably, the non-target female hippocampal mantissa in step (B) is 4-5 mantissa.
The above-mentioned non-target sea horse having laid eggs or gonadal unsaturation is to exclude the possibility of non-target female sea horse eggs flowing, thereby ensuring that eggs flowing in the breeding barrels are only from target female sea horses.
Preferably, the male hippocampus not in gestation in step (C) is: the infant's capsule develops well and is not in the male hippocampus of pregnancy. The male hippocampus is in the non-pregnant period to ensure that the male hippocampus is pregnant, so that the female hippocampus can start breeding competition as soon as the male hippocampus is imported.
Preferably, the male hippocampal mantissa of the non-pregnant period in step (C) is 1 tail.
Preferably, the male hippocampus in step (C) is 1-2 cm higher than the targeted and non-targeted female hippocampus. This is because the female hippocampus favors the male hippocampus 1-2 cm higher than itself.
Preferably, in the step (D), observing the egg flowing condition of the culture container at regular time is: after the target female hippocampus and the non-target female hippocampus are contacted, the egg flowing condition of the culture container is observed every two hours (except 18:00-06:00 at night).
Preferably, in the step (D), if the ovum at the bottom of the container is observed, the target female Hippocampus is fished for observing the belly fullness; if the abdomen is shrunken, ending the egg flowing; if the abdomen is still full, secondary egg flow is possible.
The inventors found in experiments that: the female hippocampus with full multi-tail gonads is cultivated together, and the female hippocampus basically has no egg flowing phenomenon because the breeding competition is not started (or the breeding competition strength is lower); however, once a male Hippocampus is introduced into the female Hippocampus, the phenomenon that multiple female Hippocampus simultaneously flow eggs can occur due to serious female Hippocampus eggs due to initiation of reproductive competition (or aggravated reproductive competition intensity); and the more the female-male ratio deviates from 1:1 (more female and less male), the more severe the eggs flow. Therefore, based on the principle of female hippocampus reproduction competition and the phenomenon that competition can lead to female hippocampus spawning, the invention aims to provide a method capable of inducing target female hippocampus natural spawning.
Advantageous effects
The invention has the advantage of short induction time, and after the target female sea horse contacts with the non-target female sea horse and the male sea horse, eggs can flow in two days generally; the method also has the advantage of high induction rate, and the induction rate is up to 70%; at the same time have the function of target female the sea horse has no damage. The ovum collected by the invention has the advantages of complete structure, pure components, clear quantity and the like. The invention has important significance for researching the breeding mechanism, the breeding performance, the breeding regulation and control, the artificial breeding technology and the like of the Hippocampus japonicus.
Detailed Description
The invention will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. Further, it is understood that various changes and modifications may be made by those skilled in the art after reading the teachings of the present invention, and such equivalents are intended to fall within the scope of the claims appended hereto.
Example 1
The embodiment provides a method for inducing female Hippocampus japonicus to naturally flow eggs, comprising the following steps:
A. treatment of target female hippocampus: 1, the abdomen is inflated, the gonad is full, the target female sea horse of which the ovum is to be collected is placed in a culture barrel, the height of the target female sea horse is 12-13 cm, and a thin cotton thread is tied on the neck of the target female sea horse. The cultivation barrel has a volume of 400-500L, contains 300-400L of cultivation water, a water depth of 0.8-0.9 m, a water temperature of 24-26 ℃, and the cultivation water is continuously inflated for oxygenation.
B. Competitor introduction: and (3) selecting non-target female hippocampus with 4-5 tails which have spawned and a period of days after the next spawning, and placing the non-target female hippocampus in a cultivation barrel where the target female hippocampus is located. The non-target female Hippocampus has a height of 12-13 cm, and a thin cotton thread with different color from the target female Hippocampus is tied on its neck.
C. Reproduction competition starting: 1 child-bearing bag developed well and the non-pregnant male hippocampus was placed in the cultivation barrels where the target female hippocampus and the non-target female hippocampus were located. The height of the male Hippocampus is 13-14 cm.
D. During the cultivation period, the sea horse is fed with two frozen furs or live furs every day. After contacting the female sea horses of the target and the female sea horses of the non-target, the egg flowing condition of the cultivation barrel is observed every two hours (except 18:00-06:00 at night). If the ovum is observed at the bottom of the barrel, collecting the ovum by a siphon method; in addition, the target female Hippocampus is fished for observing the belly fullness; if the abdomen is shrunken, ending the egg flowing; if the abdomen is still full, secondary egg flow is possible.
Example 2
And (3) verifying induced spawn time and induction rate:
with the method, 15 female hippocampus (12.74 + -0.86 cm) with plump gonads (i.e. 15 repeats) are placed in cultivation barrels, and 1 female hippocampus (i.e. target female hippocampus) is placed in each barrel. Then 5 non-target female seahorses and 1 male seahorse (14.22+ -0.75 cm high) were introduced into each cultivation barrel, and the egg flowing condition and time of each barrel of target female seahorse were observed. The results show that 11 target female hippocampus successfully realizes oviposition, and the induction rate is 73.3%. Of these 11 targeted female hippocampus, 1 was allowed to flow in 6 hours after contact with non-targeted female and male hippocampus, 1 was allowed to flow in 22 hours after contact, 4 was allowed to flow in 26 hours after contact, 4 was allowed to flow in 28 hours after contact, and 1 was allowed to flow in 46 hours after contact. In conclusion, after contact of the targeted and non-targeted female hippocampus, the ova flow in substantially two days.
Claims (10)
1. A method of inducing natural flow of eggs from female hippocampus, comprising the steps of:
(A) Placing the target female Hippocampus with inflated abdomen and plump gonad, with ovum to be collected in a culture container;
(B) Taking the female hippocampus which has spawned and has not been full in gonad or has not been full for a plurality of days from the next spawning as a non-target female hippocampus, and placing the non-target female hippocampus into the culture container in the step (A);
(C) Placing the male hippocampus which is not pregnant in a culture container where the target female hippocampus and the non-target female hippocampus are located;
(D) Observing the egg flowing condition of the culture container at regular time.
2. The method of claim 1, wherein the target female sea mantissa in step (a) is 1 mantissa.
3. The method of claim 1, wherein the targeted female hippocampus in step (a) is labeled to distinguish between non-targeted female hippocampus.
4. The method according to claim 1, wherein the cultivation conditions of the cultivation container in the step (a) are: the cultivation container has a volume of 400-500L, contains 300-400L of cultivation water, a water depth of 0.8-0.9 m, a water temperature of 24-26 ℃, and the cultivation water is continuously inflated for oxygenation.
5. The method of claim 1, wherein the non-target female hippocampus in step (B) is the same as the target female hippocampus.
6. The method of claim 1, wherein the non-target female hippocampal mantissa in step (B) is 4-5 mantissa.
7. The method of claim 1, wherein the male hippocampus not in gestation in step (C) is: the child-bearing bag has good development and is not in the male hippocampus of the pregnant period; the mantissa of the male hippocampus without pregnancy is 1 tail.
8. The method of claim 1, wherein the male hippocampus is 1-2 cm higher than the targeted and non-targeted female hippocampus in step (C).
9. The method of claim 1, wherein the observing the egg flowing condition of the culture container at regular time in the step (D) is: after the target female hippocampus and the non-target female hippocampus are contacted, the egg flowing condition of the culture container is observed every two hours except 18:00-06:00 of night.
10. The method of claim 1, wherein in step (D), if an ovum is observed at the bottom of the container, the targeted female hippocampus is fished for observing the belly fullness; if the abdomen is shrunken, ending the egg flowing; if the abdomen is still full, secondary egg flow is possible.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310213985.5A CN116195533B (en) | 2023-03-08 | 2023-03-08 | Method for inducing female Hippocampus japonicus to naturally flow eggs based on breeding competition principle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310213985.5A CN116195533B (en) | 2023-03-08 | 2023-03-08 | Method for inducing female Hippocampus japonicus to naturally flow eggs based on breeding competition principle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116195533A true CN116195533A (en) | 2023-06-02 |
| CN116195533B CN116195533B (en) | 2024-07-12 |
Family
ID=86512682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310213985.5A Active CN116195533B (en) | 2023-03-08 | 2023-03-08 | Method for inducing female Hippocampus japonicus to naturally flow eggs based on breeding competition principle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116195533B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020173464A1 (en) * | 2000-12-01 | 2002-11-21 | King George L. | Modulation of pericyte proliferation |
| WO2003095622A2 (en) * | 2002-05-10 | 2003-11-20 | Incyte Corporation | Proteins associated with cell growth, differentiation, and death |
| US20080242688A1 (en) * | 2007-03-19 | 2008-10-02 | Astrazeneca Ab | Method 741 |
| US20170142941A1 (en) * | 2015-07-09 | 2017-05-25 | South China Sea Institute of Oceanology | A breeding method for obtaining heterosis in lined seahorses |
| US20200229411A1 (en) * | 2016-08-21 | 2020-07-23 | Insectergy, Llc | Insect production systems and methods |
| US20210298276A1 (en) * | 2018-08-10 | 2021-09-30 | Center For Aquaculture Technologies, Inc. | A method of generating sterile and monosex progeny |
| CN114009374A (en) * | 2021-09-03 | 2022-02-08 | 中国水产科学研究院东海水产研究所 | Breeding method for promoting reproduction of male parent fish of Hippocampus japonicus in production stoppage |
-
2023
- 2023-03-08 CN CN202310213985.5A patent/CN116195533B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020173464A1 (en) * | 2000-12-01 | 2002-11-21 | King George L. | Modulation of pericyte proliferation |
| WO2003095622A2 (en) * | 2002-05-10 | 2003-11-20 | Incyte Corporation | Proteins associated with cell growth, differentiation, and death |
| US20080242688A1 (en) * | 2007-03-19 | 2008-10-02 | Astrazeneca Ab | Method 741 |
| US20170142941A1 (en) * | 2015-07-09 | 2017-05-25 | South China Sea Institute of Oceanology | A breeding method for obtaining heterosis in lined seahorses |
| US20200229411A1 (en) * | 2016-08-21 | 2020-07-23 | Insectergy, Llc | Insect production systems and methods |
| US20210298276A1 (en) * | 2018-08-10 | 2021-09-30 | Center For Aquaculture Technologies, Inc. | A method of generating sterile and monosex progeny |
| CN114009374A (en) * | 2021-09-03 | 2022-02-08 | 中国水产科学研究院东海水产研究所 | Breeding method for promoting reproduction of male parent fish of Hippocampus japonicus in production stoppage |
Non-Patent Citations (3)
| Title |
|---|
| 李继龙等: "中国水生生物种质资源价值评估研究", vol. 1, 31 May 2013, 海洋出版社, pages: 247 * |
| 王亮等: "金乌贼繁殖行为与交配策略", 生态学报, vol. 37, no. 6, 31 March 2017 (2017-03-31), pages 1871 - 1880 * |
| 闫珍珍等: "雌雄灰海马和三斑海马营养价值与功能性成分对比分析", 食品科学, 26 August 2019 (2019-08-26), pages 206 - 212 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116195533B (en) | 2024-07-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Doroshov et al. | Artificial propagation of the white sturgeon, Acipenser transmontanus Richardson | |
| Devlin et al. | Transmission and phenotypic effects of an antifreeze/GH gene construct in coho salmon (Oncorhynchus kisutch) | |
| CN102047851B (en) | Construction and stock breeding method for cultured grass carp families | |
| CN100431408C (en) | Method for breeding two hybrid species of fish | |
| CN104663547A (en) | Percocypris scaled artificial propagation method | |
| CN109090004A (en) | A kind of block type Ecology pond breeding and seedling device and method | |
| CN108142328B (en) | Early propagation and rapid seedling raising method for small yellow croakers | |
| CN101647412B (en) | Method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination | |
| CN108184727A (en) | A kind of artificial fecundation method of the former carp of crow | |
| CN107509667A (en) | A kind of channel catfish family half sibs scale construction method | |
| CN110521637A (en) | A kind of egg-shaped pompano family full-sibs construction method | |
| CN1172581C (en) | Artificial reproduction method of large salamander | |
| CN109329122B (en) | Breeding method of improved Japanese white crucian carp and establishment method of strain thereof | |
| CN114651751A (en) | Artificial fry breeding method for yellow lip fish | |
| CN110301381A (en) | A kind of artificial fecundation method of diplopore fish | |
| CN114467808A (en) | Large-scale preparation method of bighead carp grown from distant hybridization natural gynogenesis | |
| CN101584305A (en) | Method for breeding and culturing hybridized snakehead fish | |
| CN101401555B (en) | Industrialized fingerling cultivation method for late cod croaker shaped spotted maigre and special clean fish hardening agent thereof | |
| CN101669451B (en) | Method for improving production efficiency of yellow perch by hybridization with perca schrenki | |
| CN116195533B (en) | Method for inducing female Hippocampus japonicus to naturally flow eggs based on breeding competition principle | |
| CN112262794A (en) | Method for sustainable large-scale production of all-female mandarin fish | |
| CN107155971A (en) | Yi Zhong Gan fishes and black carp quasi aromatic hybrid rice breeding method | |
| CN105746398A (en) | Hatching method for fries of scaphes macrolepis | |
| CN102657123A (en) | Artificial propagation method for Thymallus arcticusgrubei Dybowski | |
| CN100512639C (en) | Plecoglossus altivelis artificial reproduction method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |