CN107509667A - A kind of channel catfish family half sibs scale construction method - Google Patents
A kind of channel catfish family half sibs scale construction method Download PDFInfo
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- CN107509667A CN107509667A CN201710826969.8A CN201710826969A CN107509667A CN 107509667 A CN107509667 A CN 107509667A CN 201710826969 A CN201710826969 A CN 201710826969A CN 107509667 A CN107509667 A CN 107509667A
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- 241000252498 Ictalurus punctatus Species 0.000 title claims abstract description 35
- 238000010276 construction Methods 0.000 title claims abstract description 15
- 238000009395 breeding Methods 0.000 claims abstract description 18
- 230000001488 breeding effect Effects 0.000 claims abstract description 18
- 235000013601 eggs Nutrition 0.000 claims abstract description 18
- 239000007924 injection Substances 0.000 claims abstract description 10
- 238000002347 injection Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 230000008775 paternal effect Effects 0.000 claims abstract description 6
- 230000013011 mating Effects 0.000 claims description 10
- 229930003451 Vitamin B1 Natural products 0.000 claims description 7
- 229960003495 thiamine Drugs 0.000 claims description 7
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 7
- 239000011691 vitamin B1 Substances 0.000 claims description 7
- 235000010374 vitamin B1 Nutrition 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 5
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 5
- 239000011976 maleic acid Substances 0.000 claims description 5
- 239000003488 releasing hormone Substances 0.000 claims description 5
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 5
- 241000251468 Actinopterygii Species 0.000 claims description 4
- 241000238557 Decapoda Species 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000017448 oviposition Effects 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 3
- 230000037396 body weight Effects 0.000 claims description 3
- 230000002710 gonadal effect Effects 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 210000000006 pectoral fin Anatomy 0.000 claims description 3
- 230000003442 weekly effect Effects 0.000 claims description 3
- 239000007928 intraperitoneal injection Substances 0.000 claims description 2
- 210000001136 chorion Anatomy 0.000 claims 1
- 230000005611 electricity Effects 0.000 claims 1
- 230000000762 glandular Effects 0.000 claims 1
- 239000005556 hormone Substances 0.000 claims 1
- 229940088597 hormone Drugs 0.000 claims 1
- 229940098895 maleic acid Drugs 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 235000019688 fish Nutrition 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 244000144972 livestock Species 0.000 description 2
- 238000009394 selective breeding Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 description 1
- 241000276701 Oreochromis mossambicus Species 0.000 description 1
- 241000157468 Reinhardtius hippoglossoides Species 0.000 description 1
- 241000277263 Salmo Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of channel catfish family half sibs scale construction method, step includes：1) parent is cultivated；2) paternal half-sib family is built；3) mate and oviposit；4) pieces of an egg are hatched, to raun injection PIT electronic markers of having laid eggs；5) artificial induced spawning.It is put into oopod after raun injection and is mated with milter；6) after the completion of laying eggs, pieces of an egg is taken out and are subsequently hatched.The female milter to have laid eggs is taken out simultaneously, is injected PIT electronic markers, is put into pond and continues to cultivate, as follow-up annual breeding.The inventive method makes channel catfish family half sibs be built into power to significantly improve, shorten family construction schedule.
Description
Technical field
The invention belongs to channel catfish breeding technical field, and in particular to a kind of channel catfish family half sibs scale
Change method for building up.
Background technology
Channel catfish (Ictalures punctatus) originates in the U.S., introduces within 1984 China's examination and supports, short
It is developed rapidly in thirties years, breaches the link technical barrier such as cultivation, breeding, feed, export processing in succession,
Form and also surge simultaneously from initial a few kiloton from seed breeding, cultivation, processing, the complete industry chain (supply chain) of the marketing, yield
Stabilization turns into one of the principal item in the China's export U.S. more than 200,000 tons.But germplasm origin cannot ensure, for many generations
The horizontal constantly rise of cultured population inbreeding, it is occurred frequently that obvious decreased growth, body-color diversity, disease occurs in cultivation channel catfish
With the uneven phenomenon for waiting germplasm decline of specification, the economic benefit of channel catfish aquaculture is had a strong impact on, kind Geological Problems are
As the restriction further sustainable development bottleneck of industry.Therefore, carry out fine-variety breeding has turned into the production of current China's channel catfish
The critical work of industry.
Genetic parameter estimation is an element task of aquatic livestock selection and use, by the estimation of breeding value, formulates choosing
Index is selected, predicts selection development.Genetic parameter is needed accurately to be estimated by family half sibs, and family half sibs quantity is got over
More, the estimation of genetic parameter is more accurate.Built both at home and abroad by extensive family half sibs and seed selection has been carried out to aquatic livestock
Through achieving great effect, such as Atlantic salmon, rainbow trout, Tilapia mossambica, Chinese prawn, turbot.The channel catfish man in China
It is that election effects development is later, establishes paternal half-sib family in genetic parameter estimation with extensive family selective breeding technology and educate
Kind solution formulation etc. is significant.Because the spermary of channel catfish milter is dendritic structure, it is impossible to as other
Fish milter equally extrudes seminal fluid and carries out artificial insemination, therefore channel catfish family structure mainly uses the side of natural propagation
Method, and based on family full-sibs.And in the family half sibs of actual implementation channel catfish, parent's gonad development be present not
Synchronous, the quality and quantity of raun spawning is unstable, causes half sibs structure inefficient and family half sibs construction schedule length
The problem of.
So far there is not yet the relevant report about channel catfish family half sibs scale construction method.
The content of the invention
A kind of the defects of it is an object of the invention to overcome prior art, there is provided channel catfish family half sibs scale
Construction method, this method is simple to operate, channel catfish family half sibs is built into power and significantly improves, shortens family structure
Build the cycle, this method can meet follow-up extensive channel catfish family selective breeding requirement.
The present invention is achieved through the following technical solutions：
A kind of channel catfish family half sibs scale construction method, its step include：
1) in natural early January in time, selection 4-10 ages, body long more than 50cm, more than body weight 2kg channel catfish
Body, by 2:1 female-male proportion collocation is put in a suitable place to breed is cultivated in parent culture pond.
2) 5-7 months then, when water temperature is higher than 24 DEG C, the channel catfish of harvesting step 1), gonadal maturation is selected
Male and female parent population, it is male with 2 female methods structure paternal half-sib familys using 1.The female milter for having neither part nor lot in mating collocation separates, and continues
Cultivation, gives over to standby.
3) designed according to breeding, first choose the production that 1 tail milter in each mating combination is put into independent tank with 1 tail raun
Mate and oviposit in egg apparatus.After the completion of combo, miniflow water and aeration are kept in tank, it is daily to observe spawning situation, production twice sooner or later
General 3-5 days of ovum time.
4) after the completion of finding spawning, pieces of an egg is taken out and are subsequently hatched.The raun that laid eggs is taken out simultaneously, is being injected intraperitoneally
PIT electronic markers, after recording mark number, it is put into pond and continues to cultivate, can be used as the breeding parent in follow-up year.
5) the another 1 tail raun of mating group is fished for, in its pectoral fin base portion injection luteotropin releasing hormone d-ala analog (LRH-
A2), human chorionic gonadtropin (HCG), vitamin B1 (VB1) and maleic acid DOM (DOM) mixture carry out artificial induced spawning, female
It is put into oopod after fish injection and is mated with milter, continues the daily morning and evening and observe spawning situation, the general 3-5 of egg-laying time twice
My god.
6) after the completion of finding spawning, pieces of an egg is taken out and are subsequently hatched.The female milter to have laid eggs is taken out simultaneously, is noted in abdominal cavity
Penetrate PIT electronic markers, after recording mark number, be put into pond and continue to cultivate, can be used as the breeding parent in follow-up year.
Channel catfish family half sibs scale construction method of the present invention, preferred steps 1) in parent cultivation
Independent cultivation is taken, breeding density is per 650-700m2150 tails are put in a suitable place to breed, further preferably per 666.7m2Put 150 tails in a suitable place to breed；Cultivation
Period in addition to mixed feed is fed, feeds weekly fresh minced fillet twice and small fish shrimp.
Channel catfish family half sibs scale construction method of the present invention, preferred steps 5) in every kilogram of spot
Cha Wei Channel-catfish injection 20ug luteotropin releasing hormone d-ala analog+1600IU human chorionic gonadtropin+1.5mg vitamin B1s+3mg
Maleic acid DOM.
Beneficial effect：
The method of the present invention, it is simple to operate, channel catfish family half sibs is built into power and significantly improve, shorten
Family construction schedule.
Embodiment
Describe the technology contents of the present invention in detail below by embodiment：
Embodiments of the invention are implemented in national channel catfish genetic breeding center (Nanjing).In
Early January then, select 4-10 ages, body long more than 50cm, more than body weight 2kg channel catfish individual, by 2:1 sex ration
Example collocation is put in a suitable place to breed is cultivated in parent culture pond.Parent culture individually cultivates, and breeding density is per 666.7m2Put 150 tails in a suitable place to breed,
In addition to mixed feed is fed, fresh minced fillet twice and small fish shrimp are fed weekly.
In June then, when water temperature is higher than 24 DEG C, the channel catfish parent of harvesting cultivation, select the female of gonadal maturation
Male parent population, paternal half-sib familys being built using 1 male 2 female methods of matching somebody with somebody, the channel catfish parent population quantity of selection is 295 tails,
The wherein tail of milter 118, the tail of raun 177.59 mating combinations are designed, often combination includes 1 tail milter and 2 tail rauns, separately there is 1 hero 1
It is female to be used as candidate, substitute and use in the female milter death of mating group.
Designed according to breeding, first choose the spawning that 1 tail milter in each mating combination is put into independent tank with 1 tail raun
Mate and oviposit in device.After the completion of 59 combos, miniflow water and aeration are kept in tank, it is daily to observe spawning situation twice sooner or later,
General 3-5 days of egg-laying time.
It was found that after the completion of spawning, take out pieces of an egg and subsequently hatched.The raun that laid eggs is taken out simultaneously, in intraperitoneal injection PIT
Electronic marker (is purchased from Qingdao Ke Yi Instrument Ltd.), after recording mark number, is put into pond and continues to cultivate, for follow-up year
Breeding.
Fish for the another 1 tail raun of mating group, its pectoral fin base portion injection luteotropin releasing hormone d-ala analog (LRH-A2),
Human chorionic gonadtropin (HCG), vitamin B1 (VB1) and maleic acid DOM (DOM) mixture carry out artificial induced spawning, per kg bodies
Injection dosage is again：20ug LRH-A2+1600IU HCG+1.5mg VB1+3mg DOM.It is put into after raun injection in oopod
Mated with milter, continue the daily morning and evening and observe spawning situation, general 3-5 days of egg-laying time twice.
It was found that after the completion of spawning, take out pieces of an egg and subsequently hatched.The female milter to have laid eggs is taken out simultaneously, is being injected intraperitoneally
PIT electronic markers, after recording mark number, it is put into pond and continues to cultivate, the breeding for follow-up year.
The present embodiment was by 10 day time, vertical paternal half-sib family 86 of building together, success rate 72.9%, compared to before
Power is built into less than 40%, and significant effect, family construction schedule also foreshortened to 10 days from original more than 3 weeks.
Claims (3)
1. a kind of channel catfish family half sibs scale construction method, it is characterised in that comprise the steps of：
1) 4-10 ages are selected in natural early January in time, body long more than 50cm, more than body weight 2kg channel catfish individual,
By 2:1 female-male proportion collocation is put in a suitable place to breed is cultivated in parent culture pond；
2) 5-7 months then, when water temperature is higher than 24 DEG C, the channel catfish of harvesting step 1), the male and female of gonadal maturation are selected
Parent population, male with 2 female methods structure paternal half-sib familys using 1, the female milter for having neither part nor lot in mating collocation separates, continues to support
Grow, give over to standby；
3) designed according to breeding, first choose the oopod that 1 tail milter in each mating combination is put into independent tank with 1 tail raun
Middle mate and oviposit, after the completion of combo, keep miniflow water and aeration in tank, it is daily to observe spawning situation twice sooner or later, during spawning
Between general 3-5 days；
4) after the completion of finding spawning, pieces of an egg is taken out and are subsequently hatched, while take out the raun that laid eggs, in intraperitoneal injection PIT electricity
Son marks, and after recording mark number, is put into pond and continues to cultivate, and can be used as the breeding parent in follow-up year；
5) the another 1 tail raun of mating group is fished for, luteotropin releasing hormone d-ala analog, chorion rush property are injected in its pectoral fin base portion
Glandular hormone, vitamin B1 and maleic acid DOM mixture carry out artificial induced spawning, are put into oopod after raun injection and are handed over milter
Match somebody with somebody, continue the daily morning and evening and observe spawning situation, general 3-5 days of egg-laying time twice；
6) after the completion of finding spawning, pieces of an egg is taken out and are subsequently hatched, while take out the female milter to have laid eggs, be injected intraperitoneally
PIT electronic markers, after recording mark number, it is put into pond and continues to cultivate, as follow-up annual breeding parent.
2. channel catfish family half sibs scale construction method according to claim 1, it is characterised in that step 1)
Independent cultivation is taken in the cultivation of middle parent, and breeding density is per 650-700m2Put 150 tails in a suitable place to breed；Removed during cultivation and feed cooperation feeding
Material is outer, feeds fresh minced fillet twice and small fish shrimp weekly.
3. channel catfish family half sibs scale construction method according to claim 1, it is characterised in that step 5)
In every kilogram of channel catfish injection 20ug luteotropin releasing hormone d-ala analog+1600IU human chorionic gonadtropins+1.5mg
Vitamin B1+3mg maleic acid DOMs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710826969.8A CN107509667A (en) | 2017-09-14 | 2017-09-14 | A kind of channel catfish family half sibs scale construction method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710826969.8A CN107509667A (en) | 2017-09-14 | 2017-09-14 | A kind of channel catfish family half sibs scale construction method |
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| Publication Number | Publication Date |
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| CN107509667A true CN107509667A (en) | 2017-12-26 |
Family
ID=60723957
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710826969.8A Pending CN107509667A (en) | 2017-09-14 | 2017-09-14 | A kind of channel catfish family half sibs scale construction method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108552086A (en) * | 2018-03-26 | 2018-09-21 | 南通华鎏水产有限公司 | A kind of channel catfish prevalent variety cultivation method |
| CN108739537A (en) * | 2018-06-06 | 2018-11-06 | 江苏省淡水水产研究所 | A kind of whitewash body colour channel catfish family producing method for seed |
| CN112335581A (en) * | 2020-10-27 | 2021-02-09 | 江苏省淡水水产研究所 | Fertilized egg for gene knockout and overexpression research of channel catfish and preparation method thereof |
| CN113854204A (en) * | 2021-11-02 | 2021-12-31 | 江苏省淡水水产研究所 | Breeding production method and system for improving egg laying rate of channel catfish |
| CN114375881A (en) * | 2022-01-24 | 2022-04-22 | 佛山市南海百容水产良种有限公司 | Efficient construction method for half-sib families of micropterus salmoides |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108552086A (en) * | 2018-03-26 | 2018-09-21 | 南通华鎏水产有限公司 | A kind of channel catfish prevalent variety cultivation method |
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| CN112335581A (en) * | 2020-10-27 | 2021-02-09 | 江苏省淡水水产研究所 | Fertilized egg for gene knockout and overexpression research of channel catfish and preparation method thereof |
| CN113854204A (en) * | 2021-11-02 | 2021-12-31 | 江苏省淡水水产研究所 | Breeding production method and system for improving egg laying rate of channel catfish |
| CN114375881A (en) * | 2022-01-24 | 2022-04-22 | 佛山市南海百容水产良种有限公司 | Efficient construction method for half-sib families of micropterus salmoides |
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Application publication date: 20171226 |