CN102726332A - Electronic marker-assisted detection method for sample size in establishing process of fish family system - Google Patents

Electronic marker-assisted detection method for sample size in establishing process of fish family system Download PDF

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CN102726332A
CN102726332A CN2012102420836A CN201210242083A CN102726332A CN 102726332 A CN102726332 A CN 102726332A CN 2012102420836 A CN2012102420836 A CN 2012102420836A CN 201210242083 A CN201210242083 A CN 201210242083A CN 102726332 A CN102726332 A CN 102726332A
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family
fish
sample size
formula
pond
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陈家长
宋超
钟立强
边文冀
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Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences
Freshwater Fisheries Research Institute of Jiangsu Province
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Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences
Freshwater Fisheries Research Institute of Jiangsu Province
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

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Abstract

The invention relates to an electronic marker-assisted detection method for a sample size in the establishing process of a fish family system. The detection method is characterized by comprising the following detection steps: a, constructing a channel catfish family system; b, carrying out breeding management on tested fish and PIT (passive integrated transpnder) marking; c, measuring characters, measuring the weight of all individual fish when the marked fish family leaves a pond, so as to acquire the average individual effective data of each fish family; d, acquiring a mean value of growth performance variation coefficients of each fish family; and e, detecting the sample size. The detection method detects the sample size required by each family in the next breeding process by calculating the mean value of the variation coefficients of each fish family built under the assist of the electronic marker (PIT) and setting the body weight growth ratio to be detected. The sample size calculated through the method can satisfy the variation generated in a fish cultivating process, so that real effects of growth performance can be prevented from being covered, and fish breeding cost can be reduced as much as possible.

Description

The auxiliary fish family of a kind of electronic marker is set up the detection method of sample size in the process
Technical field
The present invention relates to the auxiliary fish family of a kind of electronic marker and set up the detection method of sample size in the process; It mainly is average through the coefficient of variation of calculating auxiliary each family of fish set up of electronic marker; Being provided with then needs detected body weight gain ratio, thereby detects the required sample size of each family of breeding process next time.
Background technology
The method that fish breeding family commonly used is selected.Family selective breeding at first will carry out parent population to be chosen and selects, and promptly selects a tail raun and a tail milter with certain genetic background and carries out mating, and the offspring of its breeding promptly is a family.
For the growth performance of more different familys, method at present commonly used be to use electronic marker (Passive Integrated Transponder, PIT).The step of mark is, in fish bulk-growth to certain phase (generally should more than 10 grams), injects its abdominal cavity to electronic chip, and each chip has specific numbering, reads through corresponding CT scanner.Can raise together with the fish of different familys of numbering sweep record through mark together, support to certain phase (generally should more than 1 year) and carry out the comparison of family growth performance again.
The use of PIT can realize the research of the growth contrast under the identical breeding environment of different familys, compares with traditional family selective breeding, has practiced thrift the salty tremendous economic cost that brings again in the pool greatly.The expensive also extra burden that increases fish breeding of electronic chip.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point; Provide the auxiliary fish family of a kind of electronic marker to set up the detection method of sample size in the process; Set up the minimum sample size in the process through the auxiliary fish family of detected electrons mark, reduce the cost of fish breeding as far as possible
According to technical scheme provided by the invention, the auxiliary fish family of a kind of electronic marker is set up the detection method of sample size in the process, comprises following detection step:
The structure of a, channel catfish family
Obtain the basic population of channel catfish family as basic population with 5 local population channel catfish parents; Utilize the pond of independently breeding, breeding workshop, 1 male 1 female directed combo parent population, natural spawning; Each parent population pieces of an egg is 20-26 ℃ of flowing water hatching of independence incubation tank water temperature in the hatching workshop, makes up 55 of channel catfish familys altogether; Family is numbered from 1 to 55 successively;
B, management of test fish culture and PIT mark
(b.1) after young seedling is hatched 3-5 days, move into the cultivation pond in seed rearing workshop, carry out the branch pond of young seedling at ambient temperature and cultivate; With the zooplankton is open-mouthed bait, progressively feeding attractant;
(b.2) to age in days be 30 days, transfer artifical compound feed to and feed, in 55 familys, choose every family seed, move into outdoor intermediate rearing cement pit and carry out standard and divide the pond and cultivate;
(b.3) age in days to be grown is 170 days, and the individual's body quality of each family reaches 10g when above, in 55 familys, randomly draws every family fingerling and carries out individual mark identification with PIT; Family fingerling behind the mark carries out raising down with environment with the pond;
C, property determination
When the family fingerling behind the mark goes out the pond, whole individual fish body weight are measured, obtained the average individual valid data of each family fingerling;
4, the acquisition of each family growth performance coefficient of variation average of fish
The calculating of each family growth body weight coefficient of variation of fish:
CV=SD/M (formula 1)
In the formula: CV refers to the coefficient of variation; SD is a standard deviation; M is a mean value;
Each family growth body weight coefficient of variation average of fish is the result that the weight average value of each all individual fish of family is removed its standard deviation;
5, the detection of sample size
The computing formula of sample size:
t β , ( n - 1 ) = t α , ( n - 1 ) - δ × n (formula 2)
In the formula: on behalf of t, t distribute, and β refers to the class mistake, and n is meant sample size, and α is meant significance, generally gets α=0.05; δ is meant effect-size, the calculating of δ:
δ=λ/CV (formula 3)
In the formula: λ is meant the required detected body weight change ratio that the fish breeding researcher is provided with, and promptly detects ratio; λ is 15% or 20%;
The n that calculates by above-mentioned formula 1,2,3 be exactly in the test of fish family seed selection next time the minimum sample size that can accept.
The present invention refers to the zooplankton Cladocera zooplankton, opening for fish bait, commonly known as "red worms" or "fleas", including the amount straight Moina (Moina? Rectirostris), intended to lower the amount of old Daphnia ( Simocephalus? vetuloides) and so on.
The present invention has the following advantages:
The present invention is a detection method of setting up the sample size in the auxiliary fish family seed selection process of electronic marker a kind of science, feasible; The sample value that calculates through this method can satisfy the variation that produces in the fish breeding process, avoids the true effect of growth performance to be capped; Secondly, this method can be set up the minimum sample size in the process through the auxiliary fish family of detected electrons mark, reduces the cost of fish breeding as far as possible.
Embodiment
The auxiliary fish family of a kind of electronic marker of the present invention is set up the detection method of sample size in the process, comprises following prediction steps:
One, the acquisition of fish growth performance data
1, the structure of channel catfish family
5 different channel catfish geographical populations (referring to: Texas colony, Arkansas colony, Mississippi colony, Arkansas colony and Arkansas colony) that experiment is introduced with China's different time obtain channel catfish G1 from generation to generation as test material as basic population.
In May, 2009, design 25 kinds of mating combination, wherein 5 of selfing combinations in 20 of 5 geographical population intermolecular hybrid combinations, the colony utilize the pond of independently breeding, breeding workshop, 1 hero, 1 female directed combo parent population, natural spawning.Each family pieces of an egg is 20-26 ℃ of flowing water hatching of independence incubation tank water temperature in the hatching workshop, successfully makes up 55 of channel catfish familys altogether.Family is numbered from 1 to 55 successively.
2, management of test fish culture and PIT mark
On June 2nd, 2009, young seedling is hatched, and after 3-5 days, every family is got seed 1000 tails, and (3.12m * 0.92m * 0.6m) carrying out young seedling standard divides the pond cultivation in the cultivation pond in immigration seed rearing workshop.With the zooplankton is open-mouthed bait, progressively feeding attractant.To age in days be 30 days, when successfully transferring artifical compound feed to, the every family of picked at random 500 tail seeds, (3m * 2m * 1m) carries out standard and divides the pond and cultivate to move into outdoor intermediate rearing cement pit.
Age in days to be grown is about 170 days, and the individual's body quality of each family reaches 10g when above, in 55 familys, randomly draws 50 tails/family, adds up to 2750 tails, carries out individual mark with PIT (ID-100 type, Britain Trovan company produces) and discerns.Fish behind the mark carries out with the pond with the raising under the environment.
Manufactured feed of the present invention refers to No. 0 yellow cartfish manufactured feed that Ningbo Tianbang Co., Ltd produces.
Artifical compound feed of the present invention refers to No. 1 yellow cartfish manufactured feed that Ningbo Tianbang Co., Ltd produces.
3, property determination
In November 1 in 2010 during the sunrise pond, whole whose body weights are measured.Obtain the individual valid data of 1612 tails altogether, promptly each family mean is got about 30 tails of effective individuality.
4, the acquisition of each family growth performance coefficient of variation average of fish
Table 1 is the weight data of each family of fish, the data of each family of fish such as following table:
Figure BDA00001878110000041
Figure BDA00001878110000051
The computational methods of each family growth performance (body weight) coefficient of variation of fish are
CV==SD/M (formula 1)
In the formula: CV refers to the coefficient of variation; SD is a standard deviation; M is a mean value.So, each family body weight coefficient of variation average of fish is the result that all individual weight average values of each family are removed its standard deviation.
Through calculating, the average of each family body weight of channel catfish is 245.01 ± 124.97g, so coefficient of variation CV=124.97/245.01=0.51.
5, the detection of sample size
Before providing the computing formula of sample size, should illustrate the notion of effect.
Statistics effect (Statistical Power) is a probability of refusing null hypothesis reliably.Because the probability β of type is the received probability of null hypothesis of a mistake, so
Statistical Power=1-β (formula 2).
According to the definition of statistics effect, make up t and distribute.Its computing formula is
t β , ( n - 1 ) = t α , ( n - 1 ) - δ × n (formula 3)
In the formula: on behalf of t, t distribute, and β refers to the class mistake, and n is meant sample size, and α is meant significance, generally gets α=0.05; δ is meant effect-size, the calculating of δ:
δ is meant effect-size, and the computational methods of δ are:
δ=λ/CV (formula 4)
In the formula: λ is meant the required detected body weight change ratio that the fish breeding researcher is provided with, and promptly detects ratio.General λ is defaulted as 15% or 20%.
It is generally acknowledged that it is an acceptable threshold value that effect reaches 0.8.Satisfying the effect value is 0.8, the n that calculates by formula 2,3 be exactly when the experimental scheme of fish family seed selection next time the minimum sample size that can accept.
When each family of this experiment is roughly 30 samples; The relation (table 2) of the ratio of calculating according to formula 2,3,4 that detects/detect absolute value (detect absolute value=average weight * and detect ratio) and effect; Thus it is clear that, when n=30, when the effect value that reaches 0.8; The minimum ratio that detects should be between 25%-30%, and the absolute value of the body weight that can distinguish this moment is between the 61.2525-73.503g.This time result of experiment shows that the sample size of each family equals at 30 o'clock, does not reach the body weight change ratio (15% or 20%) of the required detected acquiescence of researcher's setting.Therefore, when designing the experiment of breeding next time, sample size should be increased to a suitable value.
Herein, be example with " 25% the ratio that detects obtains 0.737 effect ", specify the calculation procedure of formula.
At first, detect absolute value=average weight * and detect ratio, therefore, the ratio that detects of this moment is 245.01*25%=61.2525g.
Secondly, according to formula 4, δ=λ/CV, then δ=25%/0.51=0.49;
Then δ × n = 0.49 × 30 = 2.6849 ;
t A, (n-1)=t 0.05, (30-1)=2.045 (these results that the bilateral quantile table that distributes according to t is looked into)
T then β, (n-1)=2.045-2.6849=-0.6399
This result that the bilateral quantile table that distributes according to t is once more looked into can know β=0.526/2=0.263; So, can know Statistical Power=1-β=1-0.263=0.737 according to formula 2
The ratio that detects of table 2 channel catfish body weight/the detect relation of absolute value and effect
Figure BDA00001878110000072
Equal to detect ratio/the detect relation (table 3) of absolute value and sample size according to what formula 2,3,4 calculated at 0.8 o'clock in the effect value; It is thus clear that; The body weight change ratio that reaches acquiescence is 20%, and the sample size minimum of each family should be 55, and this moment, the corresponding absolute value that detects was 48.07g; The body weight change ratio that reaches acquiescence is 15%, and the sample size minimum of each family should be 90.Detect more little that ratio is provided with, needed sample size is big more.
Can predict that from the breeding experiment of analyzing this when the family breeding next time of design channel catfish was tested, the minimum sample size of each family should be 55.Certainly, in the experimental design stage that family is set up, when considering the sample size problem, should with PIT in the family process of growth because of mark come off and the loss of other reason is figured in, to guarantee being used for data analysis going out the enough samples of Chi Shiyou.
The ratio that detects of table 3 channel catfish body weight/the detect relation of absolute value and sample size
Bilateral quantile table described in the present invention refers to an appendix table commonly used on the statistics.β (class mistake), statistics term, finger receive the probability of wrong null hypothesis.

Claims (2)

1. an electronic marker assists fish family to set up the detection method of sample size in the process, it is characterized in that: comprise following detection step:
The structure of a, channel catfish family
Obtain the basic population of channel catfish family as basic population with 5 local population channel catfish parents; Utilize the pond of independently breeding, breeding workshop, 1 male 1 female directed combo parent population, natural spawning; Each parent population pieces of an egg is 20-26 ℃ of flowing water hatching of independence incubation tank water temperature in the hatching workshop, makes up 55 of channel catfish familys altogether; Family is numbered from 1 to 55 successively;
B, management of test fish culture and PIT mark
(b.1) after young seedling is hatched 3-5 days, move into the cultivation pond in seed rearing workshop, carry out the branch pond of young seedling at ambient temperature and cultivate; With the zooplankton is open-mouthed bait, progressively feeding attractant;
(b.2) to age in days be 30 days, transfer artifical compound feed to and feed, in 55 familys, choose every family seed, move into outdoor intermediate rearing cement pit and carry out standard and divide the pond and cultivate;
(b.3) age in days to be grown is 170 days, and the individual's body quality of each family reaches 10g when above, in 55 familys, randomly draws every family fingerling and carries out individual mark identification with PIT; Family fingerling behind the mark carries out raising down with environment with the pond;
C, property determination
When the family fingerling behind the mark goes out the pond, whole individual fish body weight are measured, obtained the average individual valid data of each family fingerling;
The acquisition of d, each family growth performance coefficient of variation average of fish
The calculating of each family growth body weight coefficient of variation of fish:
CV=SD/M (formula 1)
In the formula: CV refers to the coefficient of variation; SD is a standard deviation; M is a mean value;
The detection of e, sample size
The computing formula of sample size:
t β , ( n - 1 ) = t α , ( n - 1 ) - δ × n (formula 2)
In the formula: on behalf of t, t distribute, and β refers to the class mistake, and n is meant sample size, and α is meant significance, α=0.05; δ is meant effect-size, the calculating of δ:
δ=λ/CV (formula 3)
In the formula: λ is meant the required detected body weight change ratio that the fish breeding researcher is provided with, and promptly detects ratio; λ is 15% or 20%;
The n that calculates by above-mentioned formula 1,2,3 be exactly in the test of fish family seed selection next time the sample size that can accept.
2. the auxiliary fish family of a kind of electronic marker as claimed in claim 1 is set up the detection method of sample size in the process, it is characterized in that: each family growth body weight coefficient of variation average of said fish is the result that the weight average value of each all individual fish of family is removed its standard deviation.
CN2012102420836A 2012-07-12 2012-07-12 Electronic marker-assisted detection method for sample size in establishing process of fish family system Pending CN102726332A (en)

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Cited By (6)

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CN107509667A (en) * 2017-09-14 2017-12-26 江苏省淡水水产研究所 A kind of channel catfish family half sibs scale construction method
CN108552086A (en) * 2018-03-26 2018-09-21 南通华鎏水产有限公司 A kind of channel catfish prevalent variety cultivation method
CN111248116A (en) * 2020-01-10 2020-06-09 中国水产科学研究院珠江水产研究所 Method for evaluating influence of external acting force on fish community
CN111374081A (en) * 2020-03-16 2020-07-07 郑百成 Sturgeon underwater feeding device capable of measuring food intake and control method
CN117617157A (en) * 2023-11-15 2024-03-01 长江勘测规划设计研究有限责任公司 Method for observing death condition of in-vitro markers on fish larvae

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104335934A (en) * 2013-12-30 2015-02-11 中国科学院海洋研究所 Method for marking abalone individual
CN107509667A (en) * 2017-09-14 2017-12-26 江苏省淡水水产研究所 A kind of channel catfish family half sibs scale construction method
CN108552086A (en) * 2018-03-26 2018-09-21 南通华鎏水产有限公司 A kind of channel catfish prevalent variety cultivation method
CN111248116A (en) * 2020-01-10 2020-06-09 中国水产科学研究院珠江水产研究所 Method for evaluating influence of external acting force on fish community
CN111374081A (en) * 2020-03-16 2020-07-07 郑百成 Sturgeon underwater feeding device capable of measuring food intake and control method
CN111374081B (en) * 2020-03-16 2021-09-03 郑百成 Sturgeon underwater feeding device capable of measuring food intake and control method
CN117617157A (en) * 2023-11-15 2024-03-01 长江勘测规划设计研究有限责任公司 Method for observing death condition of in-vitro markers on fish larvae

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