CN108967285A - A kind of Parent Female of Macrobrachium rosenbergii selection - Google Patents

Abstract

The present invention relates to a kind of Parent Female of Macrobrachium rosenbergii selections.The present invention is realized " cultivation of high-quality Macrobrachium rosenbergii excellent strain " using the measurement acquisition → data analysis → Animal Model → breeding merit seed shrimp for temporarily support → establishing 100-150 half sibs and family full-sibs → standardized fluorescent pigment mark → same to environment (pond) cultivation comparative experiments → growth and survival rate index after larval culture → desalination son shrimp → viral diagnosis → standardization after different geographic populations collection → foundation basis breeding population → establishment mating system → mating acquisition female shrimp → hatching → standardization of oogenesis.

Description

A kind of Parent Female of Macrobrachium rosenbergii selection

Technical field

The present invention relates to a kind of Parent Female of Macrobrachium rosenbergii selections.

Background technique

Opaque Muscle Disease In Macrobrachium Rosenbergii is also known as white body disease, white smear disease, is the one kind for betiding the Macrobrachium rosenbergii seed stage There is muscle gonorrhoea, hickie or white smear symptom, mortality can occur within a couple of days in disease, illness shrimp body, and the death rate may be up to 60% or more, become the main harm of current Macrobrachium rosenbergii breeding, cultivation.Studies have shown that the disease of Opaque Muscle Disease In Macrobrachium Rosenbergii It originally is M.rosenbergii Nodavirus, route of transmission is based on vertical transmission, but its horizontal transmission also has extremely strong infection ability.

Summary of the invention

The present invention provide in order to solve problem above it is a kind of prevent Opaque Muscle Disease In Macrobrachium Rosenbergii prevalence, strengthen close shrimp battalion It supports, enhances its immunity, also avoid close shrimp and generate anorexia situation, increase quantity of exchanged water and change water frequency to keep the clear of water quality Newly, promote close shrimp sexual gland fast-ripenin and carry out the Parent Female of Macrobrachium rosenbergii selection of mate and oviposit in pond.

The technical scheme is that including the following steps:

1) breeding goal: select that the speed of growth is fast, opposite fish brood amount is big, emergence rate is high, the close shrimp of high survival rate is breeding goal；

2) on the basis of introducing Macrobrachium rosenbergii " South Pacific lake 2 ", 3 or more Different population conducts basic population: are collected Breeding basic population (Guangdong, Shanghai, Jiangsu)；

3) breeding facility: mating the parent shrimps and incubate young facility: 0.5 × 0.5 × 0.5 400, yarn tulle case of 60 mesh of mesh, net cage is set In in the cement pit of 12 ㎡, 20 net cages of every pond cloth, every case water body is about the 0.4(depth of water after water filling) × 0.5 × 0.5=0.1m3；

Culture facility: volume is the nursery bucket of 0.5-1.0m3 or nursery pond 200, and tool is independent to be set into draining and heating, oxygenation It applies；

Storage pond, holding pond: volume is cement pit 200 of 1.0-3.0m3, is had independent into draining and oxygenation facility；

4) breeding mates and incubate children: current year starts by the end of April, female to 5 male total fertilization failures using 1 using breeding basic population Strategy combination mating is put into mating net cage and numbers record, and family scale establishes 150-200 full sibs and family half sibs, hands over It with the close shrimp oogenesis situation of rear daily inspection and records, the female shrimp immigration of ovum will be cherished within every 7 days and incubate young net and number record, through 20 days or so Achievable mating plan；

5) larval culture and temporarily support: oogenesis parent shrimp through 15-20 days cultivation can hatchling, incubate children's generally interior completion at 1-2 days, Each inspection hatching larvae situation, the discovery young hatch i.e. progress young standardization, and each family standardization counts the young 2000 Tail is put in nursery bucket (pond) and cultivates, and main breeding condition has: 30 DEG C of water temperature, salinity 10-12 ‰, PH7.2-8.0, Z₁-Z₅It feeds Artemia nauplii, Z₅Supplement feeds egg custard afterwards；

Abnormal young shrimp and salinity can gradually be desalinated into 1 ‰ through 20-23 days or so young, can be carried out second after young shrimp desalination Standardization, counting survives young shrimp sum, and standardizes 400 tail son shrimps and be put into temporary curing cement pond；

Shrimp seedling carries out Nodavirus detection before temporarily supporting, using TAS-ELISA or RT-PCR method detection at negative shrimp seedling, ability Into storage pond, holding pond；

Cement pit temporarily supports shrimp seedling and feeds Cladocera from Freshwaters and Macrobrachium rosenbergii opening material, No. 1 material, and 25-30 DEG C of natural temperature, through 25- 30 days nurture body are long up to 2.5cm or more, and pond-inputting should be marked as early as possible at this time；

6) third time standardization and fluorochrome mark: each family shrimp seedling is recorded before label and temporarily forms motility rate, body length, weight number According to third time standardizes, and each family retains 200 tail juvenile prawns, and the raising of pond-inputting simultaneously of two ponds, Mei Gechi are divided to after the completion of label The each family in the pool respectively puts 100 tail shrimp seedlings, and breeding density is consistent with production cultivation, and insufficient section is put into unmarked shrimp and is filled out It fills；

Through pond culture in 3 months or so, to harvesting in October at shrimp, each family is distinguished and surveyed according to label at shrimp after harvesting Body length, weight data are measured, calculates survival rate, while each individual is marked using optic stalk plastic hoop；

7) data are analyzed: according to the collected data, establishing animal model using computer software, and assess family growth traits Superiority and inferiority, so that it is determined that the superior families reserved seed for planting.

The present invention is obtained using different geographic populations collection → foundation basis breeding population → establishment mating system → mating 100- temporarily support → is established after larval culture → desalination son shrimp → viral diagnosis → standardization after the female shrimp → hatching → standardization of oogenesis 150 half sibs and family full-sibs → standardized fluorescent pigment mark → same to environment (pond) cultivation comparative experiments → growth and Measurement acquisition → data analysis → Animal Model → breeding merit seed shrimp of survival rate index realizes high-quality sieve Family name's pond crayfish excellent strain is cultivated, and is improved close shrimp survival rate, is greatly improved close shrimp quality,.

Specific embodiment

A kind of Parent Female of Macrobrachium rosenbergii selection, includes the following steps:

1) breeding goal: select that the speed of growth is fast, opposite fish brood amount is big, emergence rate is high, the close shrimp of high survival rate is breeding goal；

2) on the basis of introducing Macrobrachium rosenbergii " South Pacific lake 2 ", 3 or more Different population conducts basic population: are collected Breeding basic population (Guangdong, Shanghai, Jiangsu)；

3) breeding facility: mating the parent shrimps and incubate young facility: 0.5 × 0.5 × 0.5 400, yarn tulle case of 60 mesh of mesh, net cage is set In in the cement pit of 12 ㎡, 20 net cages of every pond cloth, every case water body is about the 0.4(depth of water after water filling) × 0.5 × 0.5=0.1m3；

Culture facility: volume is the nursery bucket of 0.5-1.0m3 or nursery pond 200, and tool is independent to be set into draining and heating, oxygenation It applies；

Storage pond, holding pond: volume is cement pit 200 of 1.0-3.0m3, is had independent into draining and oxygenation facility；

4) breeding mates and incubate children: current year starts by the end of April, female to 5 male total fertilization failures using 1 using breeding basic population Strategy combination mating is put into mating net cage and numbers record, and family scale establishes 150-200 full sibs and family half sibs, hands over It with the close shrimp oogenesis situation of rear daily inspection and records, the female shrimp immigration of ovum will be cherished within every 7 days and incubate young net and number record, through 20 days or so Achievable mating plan；

5) larval culture and temporarily support: oogenesis parent shrimp through 15-20 days cultivation can hatchling, incubate children's generally interior completion at 1-2 days, Each inspection hatching larvae situation, the discovery young hatch i.e. progress young standardization, and each family standardization counts the young 2000 Tail is put in nursery bucket (pond) and cultivates, and main breeding condition has: 30 DEG C of water temperature, salinity 10-12 ‰, PH7.2-8.0, Z₁-Z₅It feeds Artemia nauplii, Z₅Supplement feeds egg custard afterwards；

Abnormal young shrimp and salinity can gradually be desalinated into 1 ‰ through 20-23 days or so young, can be carried out second after young shrimp desalination Standardization, counting survives young shrimp sum, and standardizes 400 tail son shrimps and be put into temporary curing cement pond；

Shrimp seedling carries out Nodavirus detection before temporarily supporting, using TAS-ELISA or RT-PCR method detection at negative shrimp seedling, ability Into storage pond, holding pond；

Cement pit temporarily supports shrimp seedling and feeds Cladocera from Freshwaters and Macrobrachium rosenbergii opening material, No. 1 material, and 25-30 DEG C of natural temperature, through 25- 30 days nurture body are long up to 2.5cm or more, and pond-inputting should be marked as early as possible at this time；

6) third time standardization and fluorochrome mark: each family shrimp seedling is recorded before label and temporarily forms motility rate, body length, weight number According to third time standardizes, and each family retains 200 tail juvenile prawns, and the raising of pond-inputting simultaneously of two ponds, Mei Gechi are divided to after the completion of label The each family in the pool respectively puts 100 tail shrimp seedlings, and breeding density is consistent with production cultivation, and insufficient section is put into unmarked shrimp and is filled out It fills；

Through pond culture in 3 months or so, to harvesting in October at shrimp, each family is distinguished and surveyed according to label at shrimp after harvesting Body length, weight data are measured, calculates survival rate, while each individual is marked using optic stalk plastic hoop；

7) data are analyzed: according to the collected data, establishing animal model using computer software, and assess family growth traits Superiority and inferiority, so that it is determined that the superior families reserved seed for planting.

Claims (1)

1. a kind of Parent Female of Macrobrachium rosenbergii selection, it is characterised in that: include the following steps:

1) breeding goal: select that the speed of growth is fast, opposite fish brood amount is big, emergence rate is high, the close shrimp of high survival rate is breeding goal；

2) on the basis of introducing Macrobrachium rosenbergii " South Pacific lake 2 ", 3 or more Different population conducts basic population: are collected Breeding basic population (Guangdong, Shanghai, Jiangsu)；

3) breeding facility: mating the parent shrimps and incubate young facility: 0.5 × 0.5 × 0.5 400, yarn tulle case of 60 mesh of mesh, net cage is set In in the cement pit of 12 ㎡, 20 net cages of every pond cloth, every case water body is about the 0.4(depth of water after water filling) × 0.5 × 0.5=0.1m3；

Culture facility: volume is the nursery bucket of 0.5-1.0m3 or nursery pond 200, and tool is independent to be set into draining and heating, oxygenation It applies；

Storage pond, holding pond: volume is cement pit 200 of 1.0-3.0m3, is had independent into draining and oxygenation facility；

4) breeding mates and incubate children: current year starts by the end of April, female to 5 male total fertilization failures using 1 using breeding basic population Strategy combination mating is put into mating net cage and numbers record, and family scale establishes 150-200 full sibs and family half sibs, hands over It with the close shrimp oogenesis situation of rear daily inspection and records, the female shrimp immigration of ovum will be cherished within every 7 days and incubate young net and number record, through 20 days or so Achievable mating plan；

5) larval culture and temporarily support: oogenesis parent shrimp through 15-20 days cultivation can hatchling, incubate children's generally interior completion at 1-2 days, Each inspection hatching larvae situation, the discovery young hatch i.e. progress young standardization, and each family standardization counts the young 2000 Tail is put in nursery bucket (pond) and cultivates, and main breeding condition has: 30 DEG C of water temperature, salinity 10-12 ‰, PH7.2-8.0, Z₁-Z₅It feeds Artemia nauplii, Z₅Supplement feeds egg custard afterwards；

Abnormal young shrimp and salinity can gradually be desalinated into 1 ‰ through 20-23 days or so young, can be carried out second after young shrimp desalination Standardization, counting survives young shrimp sum, and standardizes 400 tail son shrimps and be put into temporary curing cement pond；

Shrimp seedling carries out Nodavirus detection before temporarily supporting, using TAS-ELISA or RT-PCR method detection at negative shrimp seedling, ability Into storage pond, holding pond；

Cement pit temporarily supports shrimp seedling and feeds Cladocera from Freshwaters and Macrobrachium rosenbergii opening material, No. 1 material, and 25-30 DEG C of natural temperature, through 25- 30 days nurture body are long up to 2.5cm or more, and pond-inputting should be marked as early as possible at this time；

6) third time standardization and fluorochrome mark: each family shrimp seedling is recorded before label and temporarily forms motility rate, body length, weight number According to third time standardizes, and each family retains 200 tail juvenile prawns, and the raising of pond-inputting simultaneously of two ponds, Mei Gechi are divided to after the completion of label The each family in the pool respectively puts 100 tail shrimp seedlings, and breeding density is consistent with production cultivation, and insufficient section is put into unmarked shrimp and is filled out It fills；

Through pond culture in 3 months or so, to harvesting in October at shrimp, each family is distinguished and surveyed according to label at shrimp after harvesting Body length, weight data are measured, calculates survival rate, while each individual is marked using optic stalk plastic hoop；

7) data are analyzed: according to the collected data, establishing animal model using computer software, and assess family growth traits Superiority and inferiority, so that it is determined that the superior families reserved seed for planting.