CN116139053A

CN116139053A - Acne-removing repairing composition, acne-removing repairing cosmetic, preparation method and application of acne-removing repairing composition

Info

Publication number: CN116139053A
Application number: CN202211099960.9A
Authority: CN
Inventors: 李菊兰; 陈嘉欣; 邓伟健; 刘立侠
Original assignee: Youyan Dermatology Research Guangzhou Co ltd
Current assignee: Youyan Dermatology Research Guangzhou Co ltd
Priority date: 2022-09-07
Filing date: 2022-09-07
Publication date: 2023-05-23

Abstract

The invention relates to an acne-removing repairing composition, a cosmetic and a preparation method and application thereof, and belongs to the technical field of cosmetics. The acne-removing repairing composition comprises the following components in parts by weight: 0.1 to 2.5 parts of white willow bark extract, 0.1 to 1.8 parts of cortex phellodendri extract, 0.05 to 1.8 parts of radix sophorae flavescentis extract, 0.01 to 1.6 parts of olive leaf extract, 0.01 to 1.9 parts of Peruvian tamni root extract, 0.0005 to 0.005 part of antibacterial agent, 0.5 to 2.0 parts of salicylic acid and 0.01 to 2 parts of pomegranate ferment. The acne-removing repairing composition prepared by the invention can improve the skin from inside to outside in multiple dimensions of dredging pores, improving the abnormal stratum corneum and diminishing inflammation, promote the permeation of active substances to the skin, enhance the synergistic effect of the active substances, achieve the effects of mildly and efficiently removing and repairing acnes, effectively eliminate stubborn acnes and reduce the time for taking effect of removing acnes.

Description

Acne-removing repairing composition, acne-removing repairing cosmetic, preparation method and application of acne-removing repairing composition

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an acne-removing repairing composition, a cosmetic, a preparation method and application thereof.

Background

Acne commonly known as acne is a skin disease caused by obstruction of hair follicles and sebaceous glands and inflammation, and the cause of the acne is mainly as follows: (1) The oil is over-secreted, the sebum is over-secreted, sweat, dust, germ, mites and the like which are not removed in time block sebaceous gland openings; (2) local inflammation; (3) Bacterial infection, cross infection, pathogenic microorganism-acnes.

The acne-removing product commonly used in the market at present mainly removes acnes by adding salicylic acid or plant bacteriostat and other components, and mainly has the following problems: (1) Salicylic acid has certain functions of removing cutin and dredging pores, but has strong irritation to skin, and often aggravates acnes and frequently causes safety problems; (2) The acne-removing product added with the plant bacteriostatic agent has mild acne-removing effect, but has slower acne-removing effect.

Therefore, there is an urgent need to develop an anti-acne repairing composition with good effects of safety, mildness, anti-acne and repairing, which is convenient to use, can efficiently remove acnes, and has high user acceptance.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the acne-removing repairing composition with safety, mildness and good acne-removing and repairing effects, which is convenient to use, has good antibacterial, antibacterial and antioxidant properties, and has high user acceptance.

In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:

in a first aspect, the invention provides an acne-removing repairing composition, which comprises the following components in parts by weight: 0.1 to 2.5 parts of white willow bark extract, 0.1 to 1.8 parts of cortex phellodendri extract, 0.05 to 1.8 parts of radix sophorae flavescentis extract, 0.01 to 1.6 parts of olive leaf extract, 0.01 to 1.9 parts of Peruvian tamni root extract, 0.0005 to 0.005 part of antibacterial agent, 0.5 to 2.0 parts of salicylic acid and 0.01 to 2 parts of pomegranate ferment; the antibacterial agent is quaternary ammonium salt antibacterial agent or cymene.

The main active ingredient of the white willow bark extract is salicin, the salicin is an oxidase inhibitor, has the effects of controlling oil, dredging pores and inhibiting bacteria, and can be used as a natural preservative in an acne-removing repairing composition.

The cortex Phellodendri extract is yellow powder extract product of bark of cortex Phellodendri of Rutaceae, contains berberine as main ingredient, and also contains palmatine, phellodendrine, bat Ge Renjian, white bridge alkali and cortex Phellodendri tung etc., and can promote collagen production, and has anti-aging, antiinflammatory and deodorant effects.

The kuh-seng root extract contains various alkaloids, volatile oil and various mineral compounds, has different inhibition effects on various organisms and fungi, has antibacterial and anti-inflammatory effects, can effectively promote the generation of collagen by culturing in fibroblasts and the like, has anti-aging effects, and also has good effects of preserving moisture, retaining water and supplementing water.

The olive leaf extract has broad-spectrum antiviral and antibacterial effects, has a remarkable effect on infectious and malignant microorganisms, can attack pathogens only and is harmless to intestinal bacteria symbiotic to human bodies, is superior to artificial antibiotics, and has an antioxidant effect.

The Peruvian tannage extract can effectively inhibit lipid peroxidation into an antioxidant for scavenging oxygen free radicals, protect skin from being attacked by free radicals, and reduce skin sensitivity; can also reduce irritation and relieve inflammation, and has good effects in improving skin microcirculation and reducing red blood streak.

The quaternary ammonium salt antibacterial agent or the cymene is added as the antibacterial agent, so that the antibacterial effect of the acne-removing repairing composition can be improved, microbial cell membranes can be damaged, degradation among microbial cells can be promoted, decomposition of thalli can be accelerated, melanin formation can be inhibited, tyrosinase activity can be effectively inhibited, and acne marks can be prevented.

Salicylic acid dissolves the adhesive between horny layers to make the old and useless horny layers of horny layers fall off, accelerate the epidermis to update and relieve the skin keratinization abnormality; the salicylic acid can effectively remove cutin and shrink pores, can act on hair follicle wall cells, deepen the interior of the pores and loosen pore plugs, can also inhibit propionibacterium acnes, staphylococcus aureus and the like, and has the effects of mildly peeling cutin, resisting inflammation and removing acnes.

The added pomegranate ferment contains the extract of lactobacillus/pomegranate fruit fermentation product and the Leuconostoc mesenteroides/radix raphani fermentation product filtrate, so that the acne-removing repairing composition disclosed by the invention is milder in removing cutin and acne, and is beneficial to removing outer dead skin cells which can trap bacteria and sebum.

According to the invention, the white willow bark extract, the cortex phellodendri extract, the radix sophorae flavescentis extract, the olive leaf extract and the Peruvian tamni root extract are combined into a plurality of plant antibacterial repairing agents by adopting specific parts by weight, and the specific contents of the antibacterial agent, the salicylic acid and the pomegranate ferment are added, so that the components of the prepared acne-removing repairing composition have synergistic effect, and the performances of mildness, inflammation diminishing, bacteriostasis, safety and high efficiency in acne removal can be simultaneously met. In addition, the raw materials of the acne-removing repairing composition can reduce the irritation to the skin, the acne-removing effect is milder, the symptoms of skin redness, burning, itching and stinging are better improved, and the oxidation resistance of the skin can be improved.

As a preferred embodiment of the acne-removing repairing composition, the acne-removing repairing composition comprises the following components in parts by weight: 0.3 to 2 parts of white willow bark extract, 0.2 to 1 part of cortex phellodendri bark extract, 0.1 to 1.5 parts of radix sophorae flavescentis extract, 0.1 to 1 part of olive leaf extract, 0.12 to 1.5 parts of Peruvian tamni root extract, 0.001 to 0.003 part of antibacterial agent, 0.8 to 1.8 part of salicylic acid and 0.1 to 1 part of pomegranate ferment.

The inventor researches find that the acne-removing repairing composition can effectively reduce the effective time of an acne-removing product within the weight part range of the components, and has better repairing and reddening effects.

As a preferred embodiment of the acne-removing repair composition of the present invention, the acne-removing repair composition further comprises the following components in parts by weight: 0.3 to 3 parts of nicotinamide and 0.02 to 1.9 parts of chamomile extract.

27 triterpenes with anti-inflammatory effect can be separated from flos Matricariae Chamomillae, and can be used for treating dermatitis and inhibiting skin induced allergy; the chamomile extract has effects of enhancing resistance of capillary vessel, inhibiting capillary vessel permeability, relieving inflammation, scavenging free radicals, resisting oxidation, and relieving aging; the chamomile extract also has certain inhibiting effect on melanocytes, and has certain effects of whitening and removing freckle.

The inventor researches that the nicotinamide is added into the acne-removing repairing composition disclosed by the invention to assist in controlling oil, so that the skin feel is fresh and not sticky and greasy after the acne-removing repairing composition is used, the product has a quick absorption effect, and a thin protective film is formed after the acne-removing repairing composition is dried, so that the skin can be kept clean and pollution is prevented.

According to the invention, the white willow bark extract, the cortex phellodendri extract, the radix sophorae flavescentis extract, the olive leaf extract and the Peruvian tanney extract are combined into a plurality of plant antibacterial repairing agents, antibacterial agents, salicylic acid and pomegranate ferments according to a specific proportion, and the efficacy of each component can be enhanced by adding specific content of nicotinamide and golden chamomile extract, so that the acne-removing repairing composition has better effects of moisturizing, repairing, relieving and fading red, acne-removing and oxidation resistance.

In a second aspect, the invention provides the use of the above-described anti-acne repair composition in cosmetics.

In a third aspect, the invention provides a cosmetic, which is an acne-removing repair gel, wherein the acne-removing repair gel comprises the acne-removing repair composition.

As a preferred embodiment of the cosmetic according to the present invention, the cosmetic comprises the following components in parts by weight: 0.1 to 2.5 parts of white willow bark extract, 0.1 to 1.8 parts of cortex phellodendri extract, 0.05 to 1.8 parts of radix sophorae flavescentis extract, 0.02 to 1.9 parts of golden chamomile extract, 0.01 to 1.6 parts of olive leaf extract, 0.01 to 1.9 parts of Peru Latany root extract, 0.0005 to 0.005 parts of antibacterial agent, 0.5 to 2.0 parts of salicylic acid, 0.3 to 3 parts of nicotinamide, 0.01 to 2 parts of pomegranate ferment, 0.5 to 2.0 parts of thickener and 0.7 to 15 parts of humectant.

As a preferred embodiment of the cosmetic according to the present invention, the cosmetic comprises the following components in parts by weight: 0.3 to 2 parts of white willow bark extract, 0.2 to 1 part of cortex phellodendri extract, 0.1 to 1.5 parts of radix sophorae flavescentis extract, 0.05 to 1.6 parts of golden chamomile extract, 0.1 to 1 part of olive leaf extract, 0.12 to 1.5 parts of Peru taman Nib root extract, 0.001 to 0.003 part of antibacterial agent, 0.8 to 1.8 parts of salicylic acid, 0.5 to 2.5 parts of nicotinamide, 0.1 to 1 part of pomegranate ferment, 0.6 to 1.5 parts of thickener and 1.7 to 11.6 parts of humectant.

The inventor of the invention finds that the components of the acne-removing repairing gel are in the range of the weight parts, and are beneficial to improving the skin from inside to outside from a plurality of dimensions of dredging pores, improving the abnormal stratum corneum and diminishing inflammation, promoting the penetration of active substances into the skin and enhancing the efficacy of each active substance of the acne-removing repairing gel, so that the optimal acne-removing and repairing effects are achieved.

As a preferred embodiment of the cosmetic of the present invention, at least one of the following (a) to (b):

(a) The humectant is at least one of butanediol, glycerol polyether-26 and 1, 2-hexanediol;

(b) The thickener is at least one of carbomer, xanthan gum and hydroxyethyl cellulose.

In a fourth aspect, the present invention provides a method for preparing the above cosmetic, comprising the steps of:

s1, uniformly mixing water, a humectant and a thickener, homogenizing, and cooling to obtain a mixed solution A;

s2, adding the white willow bark extract, the cortex phellodendri extract, the radix sophorae flavescentis extract, the golden chamomile extract, the olive leaf extract, the Peruvian tannage extract, the antibacterial agent, the salicylic acid, the nicotinamide and the pomegranate ferment into the mixed solution A in the step S1, and uniformly mixing to obtain the cosmetic.

In a preferred embodiment of the method for producing cosmetics according to the present invention, in the step S1, the temperature of the mixing reaction is 82 to 85 ℃, the homogenizing time is 1 to 3 minutes, and the cooling temperature is 45 ℃.

Compared with the prior art, the invention has the beneficial effects that:

(1) According to the acne-removing repairing composition, specific parts by weight of white willow bark extract, cortex phellodendri extract, radix sophorae flavescentis extract, olive leaf extract and Peruvian tannage extract are combined into a plurality of plant antibacterial repairing agents, and specific contents of antibacterial agents, salicylic acid and pomegranate enzymes are added, so that the components of the prepared acne-removing repairing composition are synergistic, the skin is improved from inside to outside in multiple dimensions of dredging pores, improving the abnormal stratum corneum and diminishing inflammation, the penetration of active substances to the skin is promoted, the synergistic effect of each active substance of the acne-removing repairing composition is enhanced, the optimal and mild acne-removing and repairing effects are achieved, stubborn acnes can be effectively eliminated, and the time for acne removal to take effect is shortened;

(2) The formula of the cosmetic prepared by the acne-removing and repairing composition does not contain preservative, has mild acne-removing effect and no irritation, and can better improve the symptoms of skin redness, burning, itching and stinging;

(3) The cosmetic prepared from the acne-removing repairing composition has good oxidation resistance, can promote metabolism of cells and prevent aging of the cells, can enhance the acne-removing effect of the acne-removing repairing composition, can fade acne marks, and has a certain auxiliary effect on the removal and repair of the redness.

Detailed Description

The technical scheme of the invention is further described below by referring to examples. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. The methods or operations used in the examples, unless specifically indicated, are conventional methods or conventional operations in the art.

The PUNICA GRANATUM ferment is purchased from Active Concepts LLC, the trade name is ACB Modified Pomegranate Enzyme PF, and the ingredient is lactobacillus/PUNICA GRANATUM (PUNICA GRANATUM) fruit fermentation product extract and leuconostoc mesenteroides/radish (RAPHANUS SATIVUS) root fermentation product filtrate; in the present invention, the Peruvian tamarind root extract is purchased from VEVY EUROPE S.p.A., and is sold under the trade name ACS-ANTICTTOSTRISSOR, and the component is Peruvian tamarind (KRAMERIA TRIANDRA) root extract.

Examples 1 to 9 and comparative examples 1 to 9

The components and parts by weight of the acne-removing repair gels of examples 1 to 9 and comparative examples 1 to 9 are shown in tables 1 and 2 below. The total weight parts of the acne-removing repair gel of examples 1-9 and comparative examples 1-9 are 100 parts, and the preparation method of the acne-removing repair gel comprises the following steps:

s1, uniformly mixing water, a humectant and a thickener, heating to 83 ℃, homogenizing for 3 minutes, and cooling to 45 ℃ to obtain a mixed solution A;

s2, adding white willow bark extract, cortex phellodendri extract, radix sophorae flavescentis extract, golden chamomile extract, olive leaf extract, peruvian tannage extract, an antibacterial agent, salicylic acid, nicotinamide and pomegranate ferment into the mixed solution A in the step S1, uniformly mixing, adding a proper amount of triethanolamine, adjusting the pH value of the acne-removing repairing gel to be 6.0-6.5, and filtering to obtain the acne-removing repairing gel.

TABLE 1

TABLE 2

Effect example 1

The appearance and physicochemical properties of the acne-removing repair gels prepared in examples 1 to 9 and comparative examples 1 to 9 were tested, and the test results are shown in the following table 3, and the specific test method is as follows:

(1) Appearance test: visual observation is carried out on the acne-removing repairing gel;

(2) pH value test: mixing and diluting the acne-removing repairing gel with water, wherein the volume ratio of the acne-removing repairing gel to the water is that the acne-removing repairing gel: water=1:10, pH of the diluted solution was measured at normal temperature;

(3) Heat resistance test: preserving the temperature of the acne-removing repairing gel at 45+/-2 ℃ for 3 months, and performing physicochemical test after the acne-removing repairing gel is restored to room temperature;

(4) Cold resistance test: preserving the temperature of the acne-removing repairing gel at-18+/-2 ℃ for 3 months, and performing physicochemical test after the temperature is restored to room temperature;

(5) And (3) cyclic test: the acne-removing repairing gel is respectively kept at the temperature of 45 ℃ and the normal temperature and the temperature of minus 18 ℃ for 24 hours, circulated for three times according to the method, and subjected to physicochemical test after the room temperature is restored.

The physicochemical testing method in the steps (3) to (5) is referred to QB/T2660-2004.

TABLE 3 Table 3

As can be seen from Table 3, the acne-removing repair gels of examples 1 to 9 of the invention are weakly acidic, have a faint yellow semi-transparent to transparent paste, have no paste-like thinning phenomenon under the environments of different temperatures and different heat preservation times, and have good stability; the acne-removing repairing gels of comparative examples 1 to 9 have the phenomenon of thinning of paste under the environments of different temperatures and different times, and have poor stability.

Effect example 2

To verify the safety of the acne-removing repair gel obtained by the invention, the products obtained in examples 1 to 9 and comparative examples 1 to 9 were subjected to patch tests to verify whether there was irritation to the human body or whether the direct use efficacy test could be safely performed.

Group of subjects: 540 volunteers, 16-45 years old, 270 females, 270 males, healthy skin, no history of skin allergy. The products of the test examples 1 to 9 and the comparative examples 1 to 9 are randomly divided into 18 groups, 30 persons in each group, and the ratio of men to women in each group is 1:1, and the products of the test examples 1 to 9 and the comparative examples 1 to 9 are respectively taken as groups.

The testing method comprises the following steps: the anti-acne treatment compositions of examples 1 to 9 and comparative examples 1 to 9 were subjected to patch test according to the human skin patch test method in the cosmetic safety technical Specification, and the results were recorded according to the skin reaction classification standard, and the test results are shown in Table 4.

Skin reaction grading criteria: (1) negative reaction, grade 0; (2) suspicious reactions, only weak erythema, grade 1; (3) Weak positive response (erythema reaction), erythema, infiltration, edema, papule, grade 2; (4) A strong positive response (herpes response) with erythema, infiltration, edema, papules, herpes, which may exceed the test area, grade 3; (5) Very strong positive reactions (fusogenic herpetic reactions), with obvious erythema, severe infiltration, edema, fusogenic herpesses, reactions beyond the test area, grade 4.

TABLE 4 Table 4

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As can be seen from Table 4, the acne-removing repairing gels of examples 1 to 9, comparative example 1, comparative examples 3 to 6 and comparative examples 8 to 9 of the present invention have no skin redness or erythema and other allergic phenomena, which indicates that the product of the present invention is mild, safe and free from irritation; whereas comparative examples 2 and 7 showed weak erythema in some subjects at 24h and 48h after product use due to the high content of some of the efficacy components in the formulation.

Effect example 3

To verify the effect of the acne-removing repair gel obtained by the present invention, the products obtained in examples 1 to 9 and comparative examples 1 to 9 were subjected to a test for the effect of redness-removing repair.

Group of subjects: 180 volunteers, 20-45 vaccinia muscle groups, all had varying degrees of acne, 90 women, 90 men, and no history of skin allergy. The products of the test examples 1 to 9 and the comparative examples 1 to 9 are randomly divided into 18 groups, each group is 10 times, the ratio of men to women in each group is 1:1, and the products of the test examples 1 to 9 and the comparative examples 1 to 9 are respectively corresponding to each group.

The testing method comprises the following steps: after the face of the subject is washed by clear water, the corresponding acne-removing repairing gel is used for smearing the face, the whole face is smeared at the usage amount of 1.0+/-0.05 g/time, the whole face is smeared once in the morning and at night, the whole face is continuously used for 2 weeks, and other skin care products or products with similar effects are not used in the process; subjects with different symptoms after use were evaluated for the effect of redness and repair of the product, four symptoms (symptoms of redness, swelling, burning, itching and stinging, and no score if there is no such symptom) were scored separately, 1 highest score and 1 lowest score were removed from each group, the average score was calculated as the effect of improvement evaluation of the symptom, and the average value of the four symptom improvement effect evaluations was calculated as the integrated effect evaluation, and the evaluation results are shown in table 5.

Scoring criteria: (1) 4-5 minutes: the symptom is well repaired; (2) 3 to 4 minutes (excluding 4 minutes): the symptoms are obviously improved; (3) 2 to 3 minutes (excluding 3 minutes): the symptoms are effectively improved; (4) 1 to 2 minutes (excluding 2 minutes): the symptoms are slightly improved; (5) 0 to 1 part (excluding 1 part): the symptoms were not improved or more severe.

TABLE 5

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As can be seen from Table 5, the anti-acne repairing gel products of examples 1 to 9 of the present invention have good effects of removing red, swelling, burning, itching and stinging symptoms, and according to the comprehensive effect evaluation, the effects of removing red, repairing and repairing of the anti-acne repairing gel products of examples 1 to 9 are as follows: example 1 > example 2 > example 3 > example 4 > example 5 > example 6 > example 9 > example 8 > example 7. The evaluation of the comprehensive effects of the acne-removing and repairing gel products of comparative examples 1 to 9 is lower than that of examples 1 to 9, which shows that the gel products have poor effect of improving the symptoms of redness, burning, itching and stinging.

Effect example 4

To further verify the effect of the acne-removing repair gel obtained by the present invention, the products obtained in examples 1 to 9 and comparative examples 1 to 9 were subjected to an acne-removing effect test.

Group of subjects: 360 volunteers, 18-40 years old, all had oily skin and light/moderate acne skin, divided into 20 groups of 20, 180 females, 180 males, and no history of skin allergy. The products of the test examples 1 to 9 and the comparative examples 1 to 9 are randomly divided into 18 groups, 20 persons in each group, and the ratio of men to women in each group is 1:1, and the products of the test examples 1 to 9 and the comparative examples 1 to 9 are respectively taken as groups.

The testing method comprises the following steps: the subjects use corresponding test samples in the morning and evening every day without using other skin care products or products with similar effects, follow-up on the 0 th day, 7 th day and 14 th day of the test, test and record the acne removal condition of the volunteers after using the samples, and the same group of volunteers is completed by the same measuring personnel; the results of the anti-acne test after 14 days using the test samples are shown in table 6.

The acne removing effect is evaluated by adopting a front-back comparison, and after photographing, the sum of the non-inflammatory and inflammatory skin lesions of the face of the subject is counted, so that the curative effect is judged according to the skin lesion fading rate and the effective rate. Skin damage resolution = (number of skin damage before use-number of skin damage after use)/number of skin damage before use x 100%, and effective rate is the percentage of the sum of the number of people who are healed and significant to the total number of people tested.

Rating scale for skin lesion resolution: (1) healing: the skin damage resolution rate is more than or equal to 90 percent; (2) obvious effect: the skin damage fading rate is more than or equal to 60% and less than 90%; improvement: (3) the skin damage fading rate is more than or equal to 20 percent and less than 60 percent; (4) ineffective: the skin damage fading rate is less than 20 percent.

TABLE 6

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From table 6, it is clear that the effective rate of the acne-removing repairing gel of the embodiments 1 to 9 of the present invention can reach more than 60% when the acne-removing effect test is used on the 7 th day, and the effective rate of the acne-removing effect test is more than 75% when the acne-removing repairing gel of the embodiments 1 to 3 is used on the 14 th day, wherein the effective rate of the acne-removing repairing gel of the embodiments 1 to 3 can reach 70% when the acne-removing effect test is used on the 7 th day, and the acne-removing repairing gel of the present invention has excellent acne-removing effect performance, and effectively reduces the acne-removing effect effective time of the acne-removing repairing gel of the present invention; the effective rate of the acne-removing repairing gel of the comparative examples 1 to 9 is lower in the test of the acne-removing effect on the 7 th day and the 14 th day.

Effect example 5

To further verify the use effect of the acne-removing repair gel obtained by the invention, antibacterial effect tests were performed on the products obtained in examples 1 to 9 and comparative examples 1 to 9.

Test strain: propionibacterium acnes (ATCC 6919).

Test sample: the anti-acne repair gels obtained in examples 1 to 9 and comparative examples 1 to 9 were stored at 4℃for further use.

Culture medium: propionibacterium acnes culture medium, pH is regulated to 6.6-7.0, wherein agar 15g/L is added into the solid culture medium.

The test method comprises the following steps:

(1) Preparing and inoculating bacterial suspension: taking 0.1mL of frozen preserved bacterial suspension, culturing in 5mL of propionibacterium acnes liquid culture medium at 37 ℃ under anaerobic condition for 2 days to obtain experimental bacterial suspension;

(2) And (3) preparation of a liquid medicine: the acne-removing repairing gel is diluted to 0.1% by using normal saline, and then is diluted in a gradient way respectively. The first test gradient dilution concentrations were 100% (sample stock solution), 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78%, respectively. The concentration of the repeated gradient dilution of the second test is 10%, 8%, 6%, 4%, 2% and 1% respectively.

(3) Preparing bacterial liquid: diluting the bacterial suspension with Propionibacterium acnes liquid medium to a final bacterial concentration of 10 ⁶ CFU/mL；

(4) Culturing and judging results: the micro-holes are added with 100 mu L of bacterial liquid and 100 mu L of liquid medicine, and meanwhile, a negative control without bacterial addition and a normal growth control without liquid medicine are arranged, and 3 parallel medicines are used for each medicine, and the average value is obtained. Anaerobic incubation at 37℃was carried out and the results were observed after 48 h. Judging whether turbidity exists or not by naked eyes, and reading data by a direct method. The result is judged that the premise is that the growth control is good, the blank control is sterile and clear in growth, and other holes are inhibited along with the increase of the concentration gradient of the medicine.

The results of the first bacteriostasis gradient experiment are shown in Table 7, and the results of the second bacteriostasis gradient experiment are shown in Table 8.

TABLE 7

Note that: wherein "+" represents the growth of a fungus and "-" represents the absence.

TABLE 8

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As can be seen from tables 7 and 8, the anti-acne repairing gel products of examples 1 to 9 of the present invention have a good antibacterial effect, wherein examples 1 and 2 still have a strong antibacterial effect at a low dilution concentration. As can be seen from comparing example 1 with comparative examples 1 and 4-5, the anti-acne repairing gel product of the present invention can effectively improve the antibacterial performance of a plurality of plant antibacterial repairing agents, salicylic acid and antibacterial agents, which are composed of white willow bark extract, cortex phellodendri bark extract, radix sophorae flavescentis extract, olive leaf extract and peru ratan.

Effect example 6

To further verify the use effect of the acne-removing repair gel obtained by the invention, oxidation resistance tests were carried out on the products obtained in examples 1 to 9 and comparative examples 1 to 9 by using a DPPH radical scavenging rate measurement experiment.

The testing method comprises the following steps: the acne-removing repairing gel prepared in examples 1-9 and comparative examples 1-9 were taken to be 2mL each, and 8mL of the gel was taken to be 1X 10 ^- ⁴ mixing the DPPH-ethanol solution with mol/L, shaking uniformly, placing in a dark place at 25 ℃ for light-shielding reaction for 30min, placing the mixed solution in a centrifuge, setting 7000rpm for centrifugation for 5min,taking supernatant after centrifugation, measuring absorbance A at 517nm wavelength ₁ The method comprises the steps of carrying out a first treatment on the surface of the Taking 2mL of absolute ethyl alcohol to perform the same operation, and measuring absorbance A ₂ The method comprises the steps of carrying out a first treatment on the surface of the Taking 2mL of deionized water to perform the same operation, and measuring absorbance A ₃ The method comprises the steps of carrying out a first treatment on the surface of the Taking 2mL of vitamin C solution with the concentration of 1mg/mL as a positive control, setting 3 groups of parallel experiments for each group to calculate average value, and calculating the free radical scavenging capacity of the anti-acne repairing composition of different groups according to a DPPH scavenging rate formula, wherein the DPPH scavenging rate is = [1- (A) ₁ -A ₂ )/A ₃ ]X 100%. The test results are shown in Table 9.

TABLE 9

Project	DPPH clearance/%	Project	DPPH clearance/%
				Example 1	45.12	Comparative example 1	11.23
Example 2	44.31	Comparative example 2	18.21
				Example 3	43.25	Comparative example 3	15.36
Example 4	37.61	Comparative example 4	15.17
				Example 5	40.32	Comparative example 5	14.62
Example 6	35.9	Comparative example 6	12.13
				Example 7	38.1	Comparative example 7	13.41
Example 8	38.91	Comparative example 8	12.57
				Example 9	39.61	Comparative example 9	17.35

As can be seen from the above table, the DPPH clearance rate of the acne-removing repair gel of examples 1 to 9 is above 35%, wherein the DPPH clearance rate of example 1 is as high as 45.12%, and the oxidation resistance of comparative examples 1 to 9 is reduced due to the lack of the functional components or the large amount of part of the functional components of the acne-removing repair gel in the formulation.

Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. The acne-removing repairing composition is characterized by comprising the following components in parts by weight: 0.1 to 2.5 parts of white willow bark extract, 0.1 to 1.8 parts of cortex phellodendri extract, 0.05 to 1.8 parts of radix sophorae flavescentis extract, 0.01 to 1.6 parts of olive leaf extract, 0.01 to 1.9 parts of Peruvian tamni root extract, 0.0005 to 0.005 part of antibacterial agent, 0.5 to 2.0 parts of salicylic acid and 0.01 to 2 parts of pomegranate ferment; the antibacterial agent is quaternary ammonium salt antibacterial agent or cymene.

2. The acne-removing repair composition according to claim 1, which comprises the following components in parts by weight: 0.3 to 2 parts of white willow bark extract, 0.2 to 1 part of cortex phellodendri bark extract, 0.1 to 1.5 parts of radix sophorae flavescentis extract, 0.1 to 1 part of olive leaf extract, 0.12 to 1.5 parts of Peruvian tamni root extract, 0.001 to 0.003 part of antibacterial agent, 0.8 to 1.8 part of salicylic acid and 0.1 to 1 part of pomegranate ferment.

3. The anti-acne treatment composition of claim 1, further comprising the following components in parts by weight: 0.3 to 3 parts of nicotinamide and 0.02 to 1.9 parts of chamomile extract.

4. Use of the anti-acne repair composition according to any one of claims 1 to 3 in cosmetics.

5. A cosmetic product characterized in that it is an anti-acne repair gel comprising the anti-acne repair composition according to any one of claims 1 to 3.

6. The cosmetic product according to claim 5, wherein the cosmetic product comprises the following components in parts by weight: 0.1 to 2.5 parts of white willow bark extract, 0.1 to 1.8 parts of cortex phellodendri extract, 0.05 to 1.8 parts of radix sophorae flavescentis extract, 0.02 to 1.9 parts of golden chamomile extract, 0.01 to 1.6 parts of olive leaf extract, 0.01 to 1.9 parts of Peru Latany root extract, 0.0005 to 0.005 parts of antibacterial agent, 0.5 to 2.0 parts of salicylic acid, 0.3 to 3 parts of nicotinamide, 0.01 to 2 parts of pomegranate ferment, 0.5 to 2.0 parts of thickener and 0.7 to 15 parts of humectant.

7. The cosmetic product according to claim 6, characterized in that it comprises the following components in parts by weight: 0.3 to 2 parts of white willow bark extract, 0.2 to 1 part of cortex phellodendri extract, 0.1 to 1.5 parts of radix sophorae flavescentis extract, 0.05 to 1.6 parts of golden chamomile extract, 0.1 to 1 part of olive leaf extract, 0.12 to 1.5 parts of Peru taman Nib root extract, 0.001 to 0.003 part of antibacterial agent, 0.8 to 1.8 parts of salicylic acid, 0.5 to 2.5 parts of nicotinamide, 0.1 to 1 part of pomegranate ferment, 0.6 to 1.5 parts of thickener and 1.7 to 11.6 parts of humectant.

8. The cosmetic according to any one of claims 6 to 7, wherein at least one of the following (a) to (b):

9. The method for preparing a cosmetic according to any one of claims 6 to 8, comprising the steps of:

10. The method for preparing cosmetics according to claim 9, wherein in said step S1, said mixing temperature is 82 to 85 ℃, said homogenizing time is 1 to 3 minutes, and said cooling temperature is 45 ℃.