CN116113699A - 改造的磷酸烯醇丙酮酸羧化酶及其在提高谷氨酸棒杆菌氨基酸产量中的应用 - Google Patents

改造的磷酸烯醇丙酮酸羧化酶及其在提高谷氨酸棒杆菌氨基酸产量中的应用 Download PDF

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CN116113699A
CN116113699A CN202080105049.7A CN202080105049A CN116113699A CN 116113699 A CN116113699 A CN 116113699A CN 202080105049 A CN202080105049 A CN 202080105049A CN 116113699 A CN116113699 A CN 116113699A
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phosphoenolpyruvate carboxylase
amino acid
wild
nucleotide sequence
sequence
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皮莉
朱程军
孙悦
林丽春
邢盼盼
苏海霞
陈磊
唐鹏
王炯
左江
黄治华
李晓波
刘梦洁
蔡成平
胡小蓉
王筱蒙
杨磊
刘莎
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Wuhan Grand Hoyo Co ltd
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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Abstract

本发明提出了改造的磷酸烯醇丙酮酸羧化酶，与野生型磷酸烯醇丙酮酸羧化酶的氨基酸序列相比，所述改造的磷酸烯醇丙酮酸羧化酶的氨基酸序列的第771位和/或第931位和/或第943位氨基酸被取代。本发明通过将野生型磷酸烯醇丙酮酸羧化酶的氨基酸序列进行特定位点突变，所得到的改造的磷酸烯醇丙酮酸羧化酶对氨基酸碳流量的代谢有很大影响，利用该改造的磷酸烯醇丙酮酸羧化酶的编码基因在谷氨酸棒杆菌中表达，可以高效提高氨基酸产量，应用前景好。

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PCT国内申请，说明书已公开。

Claims (25)

  1. PCT国内申请，权利要求书已公开。
CN202080105049.7A 2020-12-24 2020-12-24 改造的磷酸烯醇丙酮酸羧化酶及其在提高谷氨酸棒杆菌氨基酸产量中的应用 Pending CN116113699A (zh)

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PCT/CN2020/139053 WO2022133917A1 (zh) 2020-12-24 2020-12-24 改造的磷酸烯醇丙酮酸羧化酶及其在提高谷氨酸棒杆菌氨基酸产量中的应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9407625A (pt) * 1993-08-24 1997-01-21 Ajinomoto Kk Carboxilase fosfoenolpiruvato mutante fragmento de DNA microorganismo DNA recombinante e processo para produzir aminoácidos
CN1196790C (zh) * 2002-12-10 2005-04-13 中国科学院遗传与发育生物学研究所 磷酸烯醇式丙酮酸羧化酶，编码该酶的基因以及该基因的应用
EP2495317A1 (en) * 2011-03-01 2012-09-05 Technische Universität Hamburg-Harburg Modified phosphoenolpyruvate carboxylase from Corynebacterium glutamicum and uses thereof
CN110195087B (zh) * 2019-05-16 2020-12-01 黑龙江伊品生物科技有限公司 用改变ppc基因的细菌发酵生产L-赖氨酸的方法
CN111206011B (zh) * 2020-03-05 2022-02-15 江南大学 一种重组谷氨酸棒杆菌及其在生产l-谷氨酸中的应用

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