CN115974625A - Bio-organic fertilizer and preparation method and application thereof - Google Patents
Bio-organic fertilizer and preparation method and application thereof Download PDFInfo
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- CN115974625A CN115974625A CN202211629867.4A CN202211629867A CN115974625A CN 115974625 A CN115974625 A CN 115974625A CN 202211629867 A CN202211629867 A CN 202211629867A CN 115974625 A CN115974625 A CN 115974625A
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- organic fertilizer
- fertilizer
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- matrine
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims description 5
- 239000003337 fertilizer Substances 0.000 claims abstract description 27
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims abstract description 23
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims abstract description 22
- 229930014456 matrine Natural products 0.000 claims abstract description 22
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 20
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 16
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 16
- 241001494479 Pecora Species 0.000 claims abstract description 12
- 210000003608 fece Anatomy 0.000 claims abstract description 12
- 239000010871 livestock manure Substances 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 235000012255 calcium oxide Nutrition 0.000 claims abstract description 10
- 239000000292 calcium oxide Substances 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 230000001737 promoting effect Effects 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 230000012010 growth Effects 0.000 claims description 9
- 240000007124 Brassica oleracea Species 0.000 claims description 8
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims description 8
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims description 8
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 8
- 244000052616 bacterial pathogen Species 0.000 claims description 7
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims description 6
- 239000013543 active substance Substances 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 239000003960 organic solvent Substances 0.000 claims description 5
- 241000813090 Rhizoctonia solani Species 0.000 claims description 4
- 241000246044 Sophora flavescens Species 0.000 claims description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 3
- 241001136249 Agriotes lineatus Species 0.000 claims description 3
- 241000220485 Fabaceae Species 0.000 claims description 3
- 241000192125 Firmicutes Species 0.000 claims description 3
- 241000223218 Fusarium Species 0.000 claims description 3
- 108010026389 Gramicidin Proteins 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 claims description 3
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 3
- 108010040201 Polymyxins Proteins 0.000 claims description 3
- 241000233639 Pythium Species 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 241001148470 aerobic bacillus Species 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 claims description 3
- RKLXDNHNLPUQRB-TVJUEJKUSA-N chembl564271 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]2C(C)SC[C@H](N[C@@H](CC(N)=O)C(=O)NC(=O)[C@@H](NC2=O)CSC1C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NC(=C)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H]1NC(=O)C(=C\C)/NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]2NC(=O)CNC(=O)[C@@H]3CCCN3C(=O)[C@@H](NC(=O)[C@H]3N[C@@H](CC(C)C)C(=O)NC(=O)C(=C)NC(=O)CC[C@H](NC(=O)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC=4C5=CC=CC=C5NC=4)CSC3)C(O)=O)C(C)SC2)C(C)C)C(C)SC1)C1=CC=CC=C1 RKLXDNHNLPUQRB-TVJUEJKUSA-N 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 210000003495 flagella Anatomy 0.000 claims description 3
- 229960004905 gramicidin Drugs 0.000 claims description 3
- ZWCXYZRRTRDGQE-SORVKSEFSA-N gramicidina Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@H](NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC=O)C(C)C)CC(C)C)C(=O)NCCO)=CNC2=C1 ZWCXYZRRTRDGQE-SORVKSEFSA-N 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims description 3
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 229960000988 nystatin Drugs 0.000 claims description 3
- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 108010082567 subtilin Proteins 0.000 claims description 3
- 235000000346 sugar Nutrition 0.000 claims description 3
- 150000008163 sugars Chemical class 0.000 claims description 3
- 241000289763 Dasygaster padockina Species 0.000 claims description 2
- 241000254062 Scarabaeidae Species 0.000 claims description 2
- 241000219784 Sophora Species 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 230000000749 insecticidal effect Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 235000008733 Citrus aurantifolia Nutrition 0.000 abstract description 3
- 235000011941 Tilia x europaea Nutrition 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 3
- 239000004571 lime Substances 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000000361 pesticidal effect Effects 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 241000255969 Pieris brassicae Species 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000002213 purine nucleotide Substances 0.000 description 2
- 125000000561 purinyl group Chemical class N1=C(N=C2N=CNC2=C1)* 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- ZSBXGIUJOOQZMP-BHPKHCPMSA-N sophoridine Chemical compound C1CC[C@@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-BHPKHCPMSA-N 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000143476 Bidens Species 0.000 description 1
- 235000010662 Bidens pilosa Nutrition 0.000 description 1
- 241001491790 Bupalus piniaria Species 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241000130993 Scarabaeus <genus> Species 0.000 description 1
- AAGFPTSOPGCENQ-UHFFFAOYSA-N Sophocarpin I Natural products C1CCC2CN3C(=O)C=CCC3C3C2N1CCC3 AAGFPTSOPGCENQ-UHFFFAOYSA-N 0.000 description 1
- IGXQFUGORDJEST-UHFFFAOYSA-N Sophocarpine Natural products O=C1C=CCC2C3CCCC4CCCC(CN12)C34 IGXQFUGORDJEST-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 238000001354 calcination Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- QMGGMESMCJCABO-JARXUMMXSA-N oxysophocarpine Chemical compound C([C@@H]1CCC[N@+]2(CCC[C@H]3[C@@H]21)[O-])N1[C@@H]3CC=CC1=O QMGGMESMCJCABO-JARXUMMXSA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- AAGFPTSOPGCENQ-JLNYLFASSA-N sophocarpine Chemical compound C1CC[C@H]2CN3C(=O)C=CC[C@@H]3[C@@H]3[C@H]2N1CCC3 AAGFPTSOPGCENQ-JLNYLFASSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a biological organic fertilizer. In 100 parts of fertilizer, 80% of residue after matrine extraction, 19.2% of decomposed sheep manure, 0.5% of bacillus subtilis and 0.3% of quicklime. Mixing, stirring and granulating to obtain the finished product of the bio-organic fertilizer. Matrine is used in the organic fertilizer raw material, so that the organic fertilizer has a pesticidal effect; the bacillus subtilis is used, so that the effects of inhibiting bacteria and promoting the proliferation of beneficial bacteria are achieved; lime is used, so that the insecticidal and bactericidal effects are achieved; the sheep manure is used and has the nutrient components of the fertilizer. Therefore, the compounded organic fertilizer has functionality and nutrition. The organic fertilizer is suitable for being used as a bio-organic fertilizer for crop planting.
Description
The application is a divisional application with the application date of 2018, 19.01.8. 201810053918.0 and the name of 'a biological organic fertilizer'.
Technical Field
The invention provides a fertilizer in the field of agriculture, and particularly provides a biological organic fertilizer, and a preparation method and application thereof.
Background
At present, the fertilizers used as crops comprise chemical fertilizers, farmyard manure and bio-organic fertilizers, relatively speaking, the bio-organic fertilizers are safe, can play a slow release role, have sufficient nutrient components, are harmless to soil and crops, have no pollution and are fully known. However, there are many biological organic fertilizers, and the effects are different according to different raw materials and proportioning efficiencies, so how to prepare the organic fertilizer with drug action needs to be researched.
Disclosure of Invention
The invention provides a biological organic fertilizer for realizing an organic fertilizer with insecticidal and bacteriostatic effects. The organic fertilizer solves the technical problem of preparing the insecticidal and bacteriostatic biological organic fertilizer by mixing the matrine, the bacillus subtilis, the sheep manure and the lime.
The technical scheme adopted by the invention for solving the technical problems is as follows:
in 100 parts of fertilizer, 80% of residue after matrine extraction, 19.2% of decomposed sheep manure, 0.5% of bacillus subtilis and 0.3% of quicklime. Mixing, stirring and granulating to obtain the finished product of the bio-organic fertilizer.
Preferably, the bacillus subtilis single cell is (0.7-0.8) × (2-3) microns, and is uniformly colored; the capsule is not contained, and the flagella is generated in the week and can move; gram-positive bacteria, wherein spores (0.6-0.9) x (1.0-1.5) are micron, oval to columnar and positioned in the center of the bacteria or slightly deviated, and the bacteria do not expand after the spores are formed; the surface of the colony is rough and opaque, and is dirty white or yellowish, and when the colony grows in a liquid culture medium, the skin becomes always formed; aerobic bacteria; decomposing tryptophan into indole by using protein, various sugars and starch; the seed-setting agent is widely distributed in soil and putrefactive organic matters and is easy to reproduce in the hay soaking juice;
active substances of subtilin, polymyxin, nystatin and gramicidin are generated in the growth process of bacillus subtilis; the active substance has obvious inhibiting effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection.
Preferably, the matrine extraction residue is residue obtained by extracting dried root, plant and fruit of Sophora flavescens ait of Leguminosae with organic solvent such as ethanol to obtain matrine; or the residue after matrine extraction is the residue after the dried root and/or the overground part of the subprostrate sophora is extracted by an ethanol organic solvent to prepare the matrine.
The invention also provides a preparation method of the biological organic fertilizer, and the biological organic fertilizer is prepared by mixing, stirring and granulating the residue after matrine extraction, decomposed sheep manure, bacillus subtilis and quick lime.
The invention also provides application of the biological organic fertilizer in cabbage planting.
Preferably, the application includes any one or two or more of the following (1) to (4):
(1) Controlling soil insects;
(2) Controlling plant root diseases;
(3) Increasing yield;
(4) Promoting the growth potential of each stage in the growth process of the cabbage.
Preferably, the pests are scarab beetles, wireworms and cutworms.
Preferably, the plant root disease is rhizoctonia solani induced rhizoctonia solani, fusarium induced root rot, and pythium induced root rot.
The invention also provides a using method of the biological organic fertilizer in the technical scheme, and the biological organic fertilizer is applied as a mouth fertilizer; the biological organic fertilizer is applied by matching with other organic fertilizers; when the biological organic fertilizer is applied as a mouth fertilizer, the dosage per mu of the biological organic fertilizer is 1000 kilograms, and the dosage per mu of other organic fertilizers is 3000 kilograms.
The organic fertilizer has the advantages that matrine is used in the raw materials of the organic fertilizer, so that the organic fertilizer has a pesticidal effect; the bacillus subtilis is used, so that the effects of inhibiting bacteria and promoting the proliferation of beneficial bacteria are achieved; lime is used, so that the insecticidal and bactericidal effects are achieved; the sheep manure is used and has the nutrient components of the fertilizer. Therefore, the compounded organic fertilizer has functionality and nutrition. The organic fertilizer is suitable for being used as a bio-organic fertilizer for crop planting.
Detailed Description
In 100 parts of fertilizer, 80% of residue after matrine extraction, 19.2% of decomposed sheep manure, 0.5% of bacillus subtilis and 0.3% of quicklime. Mixing, stirring and granulating to obtain the finished product of the bio-organic fertilizer.
And (3) fertilizer efficiency analysis:
matrine:
the matrine is prepared by extracting dried root, plant and fruit of Sophora flavescens ait of Leguminosae with organic solvent such as ethanol, and is alkaloid. The matrine is matrine, sophocarpine, oxysophocarpine, sophoridine and other alkaloids, and has the highest content. Other sources are radix Sophorae Tonkinensis and aerial parts of radix Sophorae Tonkinensis. The pure product is white powder in appearance.
The matrine pesticide used in agriculture means practically all substances extracted from sophora flavescens, such as a beggarticks extract or total matrines. The pesticide has wide application in agriculture in recent years, has good control effect, and is a low-toxicity, low-residue and environment-friendly pesticide. Mainly used for preventing and controlling various pests such as pine moth, tea moth, cabbage caterpillar and the like. Has multiple functions of insecticidal activity, bactericidal activity, plant growth regulating function and the like.
Bacillus subtilis (Bacillus subtilis), a species of Bacillus. Single cell (0.7-0.8) × (2-3) micron, coloring uniformly. Non-capsule, perigenesis flagellum, and exercise. Gram-positive bacteria, spores (0.6-0.9) x (1.0-1.5) micron, oval to columnar, located in the center of the bacteria or slightly off-center, and the bacteria do not expand after the spores are formed. The colony surface is rough and opaque, and is dirty white or yellowish, and when the colony grows in a liquid culture medium, the skin becomes always formed. Aerobic bacteria. Tryptophan can be decomposed to form indole by using protein, various sugars and starch. The application in genetic research is wide, and the research on the synthetic route of purine nucleotide and the regulating mechanism of the purine nucleotide is clear. Widely distributed in soil and putrefactive organic matters, and is easy to propagate in the Sucus subtilis extract.
Active substances such as subtilin, polymyxin, nystatin, gramicidin and the like generated in the growth process of bacillus subtilis have obvious inhibiting effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection.
The quicklime is obtained by calcining quicklime and limestone, and the quicklime is alkaline and has oxidizing property, so that the quicklime has the functions of sterilization and disinsection.
The sheep manure and sheep manure excreted by sheep belong to a thermal fertilizer, contain nitrogen, phosphorus, potassium and trace elements, and are suitable for being used as fertilizers for crops and flowers.
The examples illustrate that:
planting cabbage as a mouth fertilizer:
after ditching soil, spreading the biological organic fertilizer of the invention, applying 1000 kg per mu in combination with 3000 kg of other organic fertilizer as oral fertilizer, then sowing, after sowing, growing to maturity, and picking as commodity for sale.
In the planting process, the incidence rate of the cabbage caterpillars is observed to be 10%, and the contrast is 80%, which shows that the cabbage caterpillars have insecticidal effect. By checking the roots, the rotting rate caused by germs is basically not generated, which shows that the function of killing harmful bacteria is generated. The yield is improved by 20 percent compared with the control, which indicates that the yield is increased. The observation of each stage in the cabbage growth process shows that the cabbage growth vigor using the biological organic fertilizer is good, she Qinggen is white, and the commodity rate is improved.
The method is characterized in that:
the invention can prevent and control soil insects, such as scarab, wireworm and cutworm; and can also prevent and treat plant root diseases, such as rhizoctonia solani induced rhizoctonia rot, fusarium induced root rot, and pythium induced root rot.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (9)
1. A bio-organic fertilizer for cabbage planting is characterized in that:
in 100 parts of fertilizer, 80 percent of residue after matrine extraction, 19.2 percent of decomposed sheep manure, 0.5 percent of bacillus subtilis and 0.3 percent of quicklime.
2. The bio-organic fertilizer according to claim 1, wherein the bacillus subtilis single cell is (0.7-0.8) × (2-3) micron, and is uniformly colored; the capsule-free flagellum is generated in the week and can move; gram-positive bacteria, spores (0.6-0.9) × (1.0-1.5) micron, oval to columnar, positioned in the center of the bacteria or slightly inclined, and the bacteria do not expand after the spores are formed; the surface of the colony is rough and opaque, and is dirty white or yellowish, and when the colony grows in a liquid culture medium, the skin becomes always formed; aerobic bacteria; decomposing tryptophan into indole by using protein, various sugars and starch; the seed-setting agent is widely distributed in soil and putrefactive organic matters and is easy to reproduce in the hay soaking juice;
active substances of subtilin, polymyxin, nystatin and gramicidin are generated in the growth process of bacillus subtilis; the active substance has obvious inhibiting effect on pathogenic bacteria or pathogenic bacteria infected endogenously.
3. The bio-organic fertilizer as claimed in claim 1, wherein the residue after matrine extraction is the residue after the extraction of the dried roots, plants and/or fruits of Sophora flavescens ait of Leguminosae family by ethanol organic solvent to obtain matrine;
or the residue after matrine extraction is the residue after the dried root and/or the overground part of the subprostrate sophora is extracted by an ethanol organic solvent to prepare the matrine.
4. The method for preparing the bio-organic fertilizer as claimed in any one of claims 1 to 3, wherein the bio-organic fertilizer is prepared by mixing, stirring and granulating the residue after the matrine extraction, the decomposed sheep manure, the bacillus subtilis and the quick lime.
5. Use of the bio-organic fertilizer according to any one of claims 1 to 3 or the bio-organic fertilizer prepared by the preparation method according to claim 4 in cabbage planting.
6. The use according to claim 5, which comprises any one or two or more of the following (1) to (4):
(1) Controlling soil insects;
(2) Controlling plant root diseases;
(3) Increasing the yield;
(4) Promoting the growth potential of each stage in the growth process of the cabbages.
7. The use according to claim 6, wherein the pests are scarab beetle, wireworm and cutworm.
8. The use according to claim 6, wherein the plant root disease is rhizoctonia solani, fusarium and/or pythium root rot.
9. Use of a bioorganic fertilizer according to any one of claims 1 to 3 or produced by the process according to claim 4, wherein said bioorganic fertilizer is applied as a mouth-fertilizer;
when the biological organic fertilizer is used as a mouth fertilizer, the biological organic fertilizer is matched with other organic fertilizers for application, the dosage per mu of the biological organic fertilizer is 1000 kilograms, and the dosage per mu of the other organic fertilizers is 3000 kilograms.
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CN102807456A (en) * | 2012-08-17 | 2012-12-05 | 济南鲁泉明珠生态肥料有限公司 | Plant source sophocarpidine medicament and organic fertilizer |
CN104447035A (en) * | 2014-12-12 | 2015-03-25 | 杨凌农科大无公害农药研究服务中心 | Biological pesticide-added fertilizer and preparation method thereof |
CN105532735A (en) * | 2015-11-21 | 2016-05-04 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bactericidal and insecticidal composition containing Bacillus amyloliquefaciens and matrine |
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CN101265144B (en) * | 2008-04-18 | 2010-09-22 | 李珍发 | Technique for producing organic fertilizer capable of preventing and curing nematodiasis |
JP6568797B2 (en) * | 2013-09-16 | 2019-08-28 | アサヒカルピスウェルネス株式会社 | Sterilization of livestock litter by microorganisms |
CN104262055B (en) * | 2014-09-28 | 2016-07-06 | 山东省农业科学院科技信息研究所 | A kind of Chinese medicine slag fertilizer and production method thereof |
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CN102807456A (en) * | 2012-08-17 | 2012-12-05 | 济南鲁泉明珠生态肥料有限公司 | Plant source sophocarpidine medicament and organic fertilizer |
CN104447035A (en) * | 2014-12-12 | 2015-03-25 | 杨凌农科大无公害农药研究服务中心 | Biological pesticide-added fertilizer and preparation method thereof |
CN105532735A (en) * | 2015-11-21 | 2016-05-04 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bactericidal and insecticidal composition containing Bacillus amyloliquefaciens and matrine |
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