CN115948618A - 基于era技术的对虾桃拉综合征病毒快速检测方法及引物与探针组合 - Google Patents
基于era技术的对虾桃拉综合征病毒快速检测方法及引物与探针组合 Download PDFInfo
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- CN115948618A CN115948618A CN202211692135.XA CN202211692135A CN115948618A CN 115948618 A CN115948618 A CN 115948618A CN 202211692135 A CN202211692135 A CN 202211692135A CN 115948618 A CN115948618 A CN 115948618A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
ERA反应体系组分 | 每管加样量/μL |
溶解剂 | 20 |
正向引物(10μM) | 2.5 |
反向引物(10μM) | 2.5 |
探针(10μM) | 0.6 |
模板DNA | 2 |
无菌水 | 20.4 |
体积 | 48 |
Claims (8)
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CN202211692135.XA CN115948618A (zh) | 2022-12-28 | 2022-12-28 | 基于era技术的对虾桃拉综合征病毒快速检测方法及引物与探针组合 |
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CN202211692135.XA CN115948618A (zh) | 2022-12-28 | 2022-12-28 | 基于era技术的对虾桃拉综合征病毒快速检测方法及引物与探针组合 |
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CN115948618A true CN115948618A (zh) | 2023-04-11 |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102912041A (zh) * | 2012-11-06 | 2013-02-06 | 厦门出入境检验检疫局检验检疫技术中心 | 一种用于检测对虾五种病毒的引物、液相芯片及其用途 |
CN107988426A (zh) * | 2017-11-10 | 2018-05-04 | 杭州众测生物科技有限公司 | 对虾桃拉病毒(tsv)的raa恒温荧光检测方法及试剂 |
US20190175518A1 (en) * | 2016-04-26 | 2019-06-13 | Viaqua Therapeutics Ltd. | Compositions and methods for treating viral infections in shrimps |
CN113604607A (zh) * | 2021-07-28 | 2021-11-05 | 江苏奈尔森生物技术有限公司 | 快速检测猫疱疹病毒的era核酸试纸条扩增试剂盒及制备方法和检测方法 |
CN114015809A (zh) * | 2021-12-02 | 2022-02-08 | 中国检验检疫科学研究院 | 快速检测gi群诺如病毒的era方法、组合物和试剂盒 |
-
2022
- 2022-12-28 CN CN202211692135.XA patent/CN115948618A/zh active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102912041A (zh) * | 2012-11-06 | 2013-02-06 | 厦门出入境检验检疫局检验检疫技术中心 | 一种用于检测对虾五种病毒的引物、液相芯片及其用途 |
US20190175518A1 (en) * | 2016-04-26 | 2019-06-13 | Viaqua Therapeutics Ltd. | Compositions and methods for treating viral infections in shrimps |
CN107988426A (zh) * | 2017-11-10 | 2018-05-04 | 杭州众测生物科技有限公司 | 对虾桃拉病毒(tsv)的raa恒温荧光检测方法及试剂 |
CN113604607A (zh) * | 2021-07-28 | 2021-11-05 | 江苏奈尔森生物技术有限公司 | 快速检测猫疱疹病毒的era核酸试纸条扩增试剂盒及制备方法和检测方法 |
CN114015809A (zh) * | 2021-12-02 | 2022-02-08 | 中国检验检疫科学研究院 | 快速检测gi群诺如病毒的era方法、组合物和试剂盒 |
Non-Patent Citations (3)
Title |
---|
ARUN K. DHAR ET AL.: "Quantitative assay for measuring the Taura syndrome virus and yellow head virus load in shrimp by real-time RT-PCR using SYBR Green chemistry", JOURNAL OF VIROLOGICAL METHODS, vol. 104, pages 69 - 82, XP002461526, DOI: 10.1016/S0166-0934(02)00042-3 * |
李伟哲 等: "桃拉综合征病毒的环介导等温扩增联合横向流动试纸条(LAMP-LFD)检测方法的建立", 河北农业大学学报, vol. 43, no. 3, pages 81 * |
王帅 等: "RPA、RAA及ERA技术在食源性致病菌快速检测中的研究进展", 食品科学, pages 1 * |
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Effective date of registration: 20240218 Address after: 572024 7th floor, building 1, UFIDA Industrial Park, Yazhou Bay science and Technology City, Yazhou District, Sanya City, Hainan Province Applicant after: Sanya Institute of Oceanography Ocean University of China Country or region after: China Applicant after: HEIBEI XINHAI AQUATIC BIOTECHNOLOGY Co.,Ltd. Address before: 572024 Building 10, Yonyou Industrial Park, Yazhou Bay Science and Technology City, Yazhou District, Sanya City, Hainan Province Applicant before: Sanya Institute of Oceanography Ocean University of China Country or region before: China |